CN110577441A - Amino acid zinc type plant nutrient compounded by streptomyces hygroscopicus metabolite adenine-9-D-anhydro glucoside fermentation liquid - Google Patents
Amino acid zinc type plant nutrient compounded by streptomyces hygroscopicus metabolite adenine-9-D-anhydro glucoside fermentation liquid Download PDFInfo
- Publication number
- CN110577441A CN110577441A CN201910933607.8A CN201910933607A CN110577441A CN 110577441 A CN110577441 A CN 110577441A CN 201910933607 A CN201910933607 A CN 201910933607A CN 110577441 A CN110577441 A CN 110577441A
- Authority
- CN
- China
- Prior art keywords
- amino acid
- streptomyces hygroscopicus
- vitamin
- parts
- zinc
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B15/00—Organic phosphatic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Abstract
A plant nutrient is prepared from the fermented liquid of the metabolite adenine-9-D-aminogou glucoside of Streptomyces hygroscopicus through proportionally mixing it with the fermented liquid of the metabolite of Streptomyces hygroscopicus LZ-17, compound zinc amino acid, compound amino acid nutrient reinforcer, peptone and water. The problem of traditional agricultural product fresh-keeping after gathering poor, adopt chemical agent fresh-keeping to have the potential safety hazard is solved. The plant nutrient obtained by the method can promote the root system development of crops, meet the nutrient requirements of various stages in the growth process, achieve the effects of preventing and treating diseases, increasing yield, and being safe and environment-friendly through the interaction of the metabolic products of the streptomyces hygroscopicus LZ-17 strain, the zinc amino acid and the amino acid nutrition enhancer, has obvious disease-resistant and yield-increasing effects on the crops, reduces the residual risk of agricultural products, prolongs the quality guarantee period of the agricultural products, reduces the loss of the agricultural products, increases the agricultural income, and obtains good economic and social benefits.
Description
Technical Field
The invention relates to a plant regulation and control technology, in particular to a plant nutrient prepared by compounding Streptomyces hygroscopicus metabolite adenine-9-D-anhydrobioside fermentation liquor with amino acid zinc.
Background
The nutrient substances and elements containing chemical synthetic hormones are applied for years in China to cause serious pollution to agricultural products, planting soil, surface water and the like, and particularly, the agricultural products are safe to food, the physical and chemical properties of the soil are deteriorated, and plant diseases are serious. In order to realize zero growth of disease-resistant fertilizer and development of green agriculture and ecological restoration agriculture in 2020, novel regulatory plant nutrient is developed towards non-toxicity, functionalization, ecological compatibility and restoration in the future.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides the amino acid zinc type plant nutrient compounded by the streptomyces hygroscopicus metabolite adenine-9-D-angou glucoside fermentation liquid, which meets the requirements of various stages on nutrient substances, elements and disease resistance in the growth process of crops.
The technical problem to be solved by the invention is realized by the following technical scheme, the amino acid zinc type plant nutrient is compounded by using streptomyces hygroscopicus metabolite adenine-9-D-angou glucoside fermentation liquor, and is characterized by being mainly prepared from the following raw materials in parts by weight:
1-90 parts of streptomyces hygroscopicus metabolite adenine-9-D-anhydrobiose fermentation liquor,
4-70 parts of compound amino acid zinc,
1-20 parts of peptone,
10-90 parts of water, namely,
mixing the above materials, sterilizing at 60 ~ 70 deg.C, and cooling to obtain mixed solution.
The technical problem to be solved by the invention can be further realized by the following technical scheme, and the raw materials also comprise 1-20 parts by weight of compound amino acid and 1-20 parts by weight of nutrition enhancer.
The technical problem to be solved by the invention can be further realized by the following technical scheme, and the raw materials also comprise 1-10 parts of secondary elements and 1-10 parts of trace elements.
The technical problem to be solved by the invention can be further realized by the following technical scheme, wherein the amino acid zinc comprises: one or the combination of more than two of zinc glycinate, zinc lysine, zinc tryptophan, zinc proline, zinc methionine, zinc glutamate, zinc alanine and zinc leucine, wherein the total weight part of the zinc amino acid is not less than 2%.
The technical problem to be solved by the present invention can be further solved by the following technical solutions, wherein the compound amino acid comprises: glycine, alanine, valine, leucine, isoleucine, phenylalanine, tryptophan, tyrosine, aspartic acid, histidine, asparagine, glutamic acid, lysine, glutamine, methionine, arginine, serine, threonine, cysteine, proline or a combination of two or more thereof.
The technical problem to be solved by the invention can be further realized by the following technical scheme, wherein the nutrition enhancer comprises: vitamin B1, vitamin B2, vitamin B3, vitamin B4, vitamin B5, vitamin B6, vitamin B7, vitamin B11, vitamin B12, vitamin C, vitamin D, vitamin E, vitamin F, vitamin K, vitamin P, vitamin T, vitamin U, vitamin A, inositol, choline chloride, ketoglutaric acid, melatonin, sugar alcohol calcium, polyamino acids, small animal peptides, oligosaccharide, chitosan, nucleotide, magnesium sulfate, ferrous sulfate, ferric amino acid, copper sulfate, copper amino acid, manganese sulfate, manganese amino acid, zinc sulfate, and potassium silicate.
The technical problem to be solved by the invention can be further realized by the following technical scheme, wherein the trace elements comprise: iodine, boron, cobalt, molybdenum, titanium, lanthanum compounds or organic compounds.
The technical problem to be solved by the invention can be further realized by the following technical scheme, the streptomyces hygroscopicus metabolite adenine-9-D angou glucoside fermentation broth is fermentation broth obtained by taking streptomyces hygroscopicus LZ-17 strain as a starting strain to obtain metabolite adenine-9-D angou glucoside, and completely metabolizing the streptomyces hygroscopicus LZ-17 strain to obtain the metabolite adenine-9-D angou glucoside.
The technical problem to be solved by the invention can be further realized by the following technical scheme that the streptomyces hygroscopicus LZ-17 strain is activated and then inoculated into a glucose aspartyl acid culture medium, wherein the culture medium comprises 8 ~ 12g of glucose, 0.5 ~ 1g of aspartyl acid, 0.5 ~ 1g of dipotassium hydrogen phosphate, 150 ~ 250mg/L of threonine and 1000mL of distilled water in a 2000L fermentation tank, and the threonine is fed with materials for fermentation for 72 hours.
the technical problem to be solved by the invention can be further realized by the following technical scheme that the fermentation liquor of the metabolic product adenine-9-D-angou glucoside of the streptomyces hygroscopicus needs to be inactivated and cracked before being homogenized and mixed, specifically, the fermentation liquor of the metabolic product adenine-9-D-angou glucoside of the streptomyces hygroscopicus is heated to 65 ~ 100 ℃ for inactivation and cracking, and is filtered by a nano filter core, insoluble substances are removed, and then the fermentation liquor is cooled aseptically.
Compared with the prior art, the invention has the following beneficial effects:
(1) The metabolic product adenine-9-D-angou glucoside of the streptomyces hygroscopicus in the regulatory plant nutrient has the functions of regulating the growth performance of plants, promoting the generation of plant roots, increasing the leaf area, promoting photosynthesis and obviously increasing the yield. Experiments show that the plant growth regulator has the effects of promoting healing of injured tissues, promoting development of weak seedling root systems, recovering aged root systems, promoting the oxidation resistance and damage resistance of cells, preventing and treating premature senility of crops and improving the quality of the crops; particularly, the weight of a single fruit of the fruit is increased, the sugar degree is increased, and the hardness of the fruit is increased.
(2) The regulating plant nutrient can meet the requirements of various stages in the crop growth process on nutrient substances, elements and disease resistance, is safe to crops and environment, and has the effects of promoting the crop growth and inhibiting pathogenic microorganisms. The composition has good prevention and treatment effects on various plant diseases such as downy mildew, rot, late blight, early blight, alternaria leaf spot, rice blast, gray mold, fusarium wilt, false smut, banded sclerotial blight, bacterial streak, root rot, scab, black spot, sclerotinia rot, ginseng root rot, gummosis, canker, gibberellic disease, root rot, anthracnose, small spot, large spot and tobacco brown spot. The invention is nontoxic and safe to environment, human and livestock, has no residue, and belongs to an ecological recovery type regulation plant nutrient.
(3) The invention can change the soil hardening condition caused by salinization after long-term use, improve the plant growth environment, effectively inhibit the growth and reproduction of harmful microorganisms at the rhizosphere of crops, reduce the harm of soil toxins to the root systems of the crops, prevent soil-borne diseases aggravated by continuous cropping of the crops, obviously increase bacteria and actinomycetes, reduce the number of fungi and restore the self-repairing capability of the soil. The invention improves the absorption and running capability of trace elements in plants, effectively promotes the healthy growth of crops, and has remarkable promoting effects on rooting, growth, reproduction, pollination, fruiting, expansion and the like of the plants. The regulating plant nutrient has obvious disease-resistant and yield-increasing effects on crops.
The preparation process of the regulatory plant nutrient is advanced and scientific, no three wastes are generated, and the discharge meets the environmental protection requirement.
the zinc amino acid plant nutrient of adenine-9-D-angou glycoside is a novel ecological compound plant nutrient with plant physiological regulation, disease resistance and bacteriostasis efficacies, which is formed by mixing a certain nutrient substance and a soil microecology promoting substance according to a formula and adding adenine-9-D-angou glycoside, amino acid and a nutrition enhancer into a specific compound system through a certain process technology. Through field use effect experiments and toxicological tests, the zinc amino acid plant nutrient of adenine-9-D-angou glucoside has no safety risk to people and livestock. And can restore the ecology of soil, promote the growth of earthworms, inhibit plant pathogenic bacteria, and improve the total amount of bacillus mucilaginosus potassium bacteria, azotobacter chroococcum, azotobacter azedarach, pseudomonas fluorescens phosphorus bacteria and bacillus megaterium phosphorus bacteria in the soil. The yield of leaf vegetable crops is improved by 17%, the yield of strawberries is improved by 27%, the yield of watermelons is improved by 29%, the yield of pomegranates is improved by 30%, and the yield of grapes is improved by 26%.
Detailed Description
Embodiments of the present invention will be described in detail below with reference to examples, but it will be understood by those skilled in the art that the following examples are only illustrative of the present invention and should not be construed as limiting the scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.
The compound amino acid zinc type plant nutrient prepared by using streptomyces hygroscopicus metabolite adenine-9-D-anhydro-glucoside fermentation liquor is characterized by mainly being prepared from the following raw materials in parts by weight:
1-90 parts of streptomyces hygroscopicus metabolite adenine-9-D-anhydrobiose fermentation liquor,
4-70 parts of compound amino acid zinc,
1-20 parts of peptone,
10-90 parts of water, namely,
Mixing the above materials, sterilizing at 60 ~ 70 deg.C, and cooling to obtain mixed solution.
The raw materials also comprise 1-20 parts by weight of compound amino acid and 1-20 parts by weight of nutrition enhancer.
The raw materials also comprise 1-10 parts of secondary elements and 1-10 parts of trace elements.
the amino acid zinc comprises: one or the combination of more than two of zinc glycinate, zinc lysine, zinc tryptophan, zinc proline, zinc methionine, zinc glutamate, zinc alanine and zinc leucine, wherein the total weight part of the zinc amino acid is not less than 2%.
The compound amino acid comprises: glycine, alanine, valine, leucine, isoleucine, phenylalanine, tryptophan, tyrosine, aspartic acid, histidine, asparagine, glutamic acid, lysine, glutamine, methionine, arginine, serine, threonine, cysteine, proline or a combination of two or more thereof.
the nutrition enhancer comprises: vitamin B1, vitamin B2, vitamin B3, vitamin B4, vitamin B5, vitamin B6, vitamin B7, vitamin B11, vitamin B12, vitamin C, vitamin D, vitamin E, vitamin F, vitamin K, vitamin P, vitamin T, vitamin U, vitamin A, inositol, choline chloride, ketoglutaric acid, melatonin, sugar alcohol calcium, polyamino acids, small animal peptides, oligosaccharide, chitosan, nucleotide, magnesium sulfate, ferrous sulfate, ferric amino acid, copper sulfate, copper amino acid, manganese sulfate, manganese amino acid, zinc sulfate, and potassium silicate.
The trace elements include: iodine, boron, cobalt, molybdenum, titanium, lanthanum compounds or organic compounds.
The fermentation liquor of the metabolite adenine-9-D-angou glucoside of the streptomyces hygroscopicus is fermentation liquor obtained by taking a strain of streptomyces hygroscopicus LZ-17 as a starting strain to obtain the metabolite adenine-9-D-angou glucoside, and fermenting the strain of streptomyces hygroscopicus LZ-17 after completely metabolizing the strain.
The Streptomyces hygroscopicus LZ-17 strain belongs to the water-absorbing group of Streptomyces, is identified as Streptomyces hygroscopicus, and is the strain of Streptomyces hygroscopicus LZ-17 with laboratory number. The method of the invention uses the water-absorbing streptomyces LZ-17 as an initial strain to obtain the metabolite adenine-9-D-angou glucoside, the substance is found to have plant cell physiological activity and the effect similar to plant cytokinin, and no toxic toxicological reaction is caused by 2.5g/kg of mouse intraperitoneal injection, thus meeting the judgment of nontoxic substances.
The streptomyces hygroscopicus LZ-17 strain is activated and inoculated into a glucose aspargine culture medium, the culture medium comprises 8 ~ 12g of glucose, 0.5 ~ 1g of aspargine, 0.5 ~ 1g of dipotassium phosphate, 150 ~ 250mg/L of threonine and 1000mL of distilled water in a 2000L fermentation tank, and the threonine is fed with a feed for fermentation for 72 hours.
The fermentation liquor of the metabolite adenine-9-D-angou glucoside of the streptomyces hygroscopicus needs to be inactivated and cracked before being homogenized and mixed, and specifically, the fermentation liquor of the metabolite adenine-9-D-angou glucoside of the streptomyces hygroscopicus is heated to 65 ~ 100 ℃ for inactivation and cracking, filtered by a nano filter element, and subjected to aseptic cooling after insoluble substances are removed.
The metabolite has the activities of promoting plant growth and resisting premature senility, forms convertible purine substances with cell activity in the fermentation production process, has the functions of promoting healing and regeneration of crop tissues after being subjected to trauma, enhances the photosynthesis of crops, and has obvious yield increase. Meanwhile, the metabolite can promote the development of weak seedling root systems and the recovery of aged root systems, improve the disease resistance of crops and optimize the quality of the crops. The metabolite promotes plant growth, and is nontoxic and safe to human and livestock.
The zinc amino acid, the nutrition-enriched substance and the trace element are substances which are efficiently utilized and absorbed by plants and participate in physiological functions, and all effective physiological metabolic elements, protein synthesis, photosynthetic electron transfer, free radical damage resistance of cells and enzyme activity promotion are provided for crops, and the important technical guarantee for comprehensively improving the activity of plant functional enzyme and promoting the meristem of cells to generate organs is provided.
The preparation method comprises the following steps:
Weighing the raw materials according to the weight parts, and mixing to obtain liquid nutrient substances and elements.
As a further improvement, the fermentation liquor of the metabolite adenine-9-D-angou glucoside of streptomyces hygroscopicus also comprises the steps of inactivation, cracking and filtration to remove insoluble substances before mixing.
Dissolving amino acid zinc, peptone, amino acid, nutrition enhancing substances and trace elements in the formula into water or fermentation liquor of the formula amount, mixing with the inactivated, cracked and filtered fermentation liquor of the metabolic product adenine-9-D-anhydro glycoside of streptomyces hygroscopicus, stirring uniformly, sterilizing at 60 ~ 70 ℃, and cooling;
And detecting and packaging to obtain the product.
in a preferred embodiment, the plant regulating nutrient containing the streptomyces hygroscopicus metabolite adenine-9-D-anhydro-glycoside amino acid zinc type is mainly prepared from the following raw materials in parts by weight: 54 parts of fermentation liquor of metabolite of streptomyces hygroscopicus LZ-17 strain, 4 parts of compound amino acid zinc, 10 parts of peptone, 10 parts of medium element, 1 part of microelement, 1 part of compound vitamin and 10 parts of sugar. 10 parts of amino acid, and sterilizing and cooling the mixture at 65 ℃ after the mixture is homogenized and mixed to obtain a solution.
In a preferred embodiment, the amino acid zinc plant nutrient containing the streptomyces hygroscopicus metabolite adenine-9-D-anhydro-glycoside is mainly prepared from the following raw materials in parts by weight: 50 parts of fermentation liquor of metabolite of streptomyces hygroscopicus LZ-17 strain, 4 parts of compound amino acid zinc, 10 parts of compound amino acid, 5 parts of nutrition enhancer, 5 parts of urea phosphate, 5 parts of potassium sulfate, 2 parts of choline chloride, 1 part of ketoglutaric acid, 1 part of compound vitamin, 10 parts of peptone and 7 parts of water, and sterilizing and cooling at 65 ℃ after homogenizing and mixing to obtain a solution.
As a preferred embodiment, the amino acid zinc plant nutrient containing the Streptomyces hygroscopicus metabolite adenine-9-D-anhydro-glycoside is mainly prepared from the following raw materials in parts by weight: 50 parts of fermentation liquor of metabolite of streptomyces hygroscopicus LZ-17 strain, 4 parts of compound amino acid zinc, 10 parts of compound amino acid, 10 parts of nutrition enhancer, 5 parts of urea phosphate, 5 parts of potassium sulfate, 5 parts of choline chloride, 1 part of ketoglutaric acid, 1 part of compound vitamin and 9 parts of peptone. After homogenizing and mixing, sterilizing at 65 ℃ and cooling to obtain the solution.
By further optimizing the formula, the disease-resistant and yield-increasing effects of the regulatory plant nutrient can be further improved.
For a further understanding of the present invention, the components, methods and effects of the present invention are described in further detail below with reference to specific examples.
Example 1
Preparing a streptomyces hygroscopicus metabolite adenine-9-D-anhydro-amino-acid zinc type plant nutrient, weighing the following raw materials in parts by weight:
54 parts of fermentation liquor of metabolite of streptomyces hygroscopicus LZ-17 strain, 4 parts of compound amino acid zinc, 10 parts of peptone, 10 parts of secondary element, 1 part of microelement, 1 part of compound vitamin, 10 parts of sugar and 10 parts of amino acid, homogenizing, mixing, sterilizing at 65 ℃, and cooling to obtain solution.
The preparation method comprises the following steps:
(1) Heating, extinguishing, cracking and filtering the fermentation broth of the metabolic product adenine-9-D-anhydro-glycoside of streptomyces hygroscopicus obtained by fermentation, removing insoluble substances, and cooling aseptically for later use, wherein 4 parts of compound amino acid zinc, 10 parts of peptone, 10 parts of medium element, 1 part of trace element, 1 part of compound vitamin, 10 parts of sugar and 10 parts of amino acid are dissolved to obtain a solution A;
(2) And (3) adding 54 parts of fermentation liquor into the solution A obtained in the step (1), and mixing, homogenizing and stirring uniformly to obtain a product.
Example 2
Preparing a streptomyces hygroscopicus metabolite adenine-9-D-anhydro-amino-acid zinc type plant nutrient, weighing the following raw materials in parts by weight:
50 parts of fermentation liquor of metabolite of streptomyces hygroscopicus LZ-17 strain, 4 parts of compound amino acid zinc, 10 parts of compound amino acid, 5 parts of nutrition enhancer, 5 parts of urea phosphate, 5 parts of potassium sulfate, 2 parts of choline chloride, 1 part of ketoglutaric acid, 1 part of compound vitamin, 10 parts of peptone and 7 parts of water, and sterilizing and cooling at 65 ℃ after homogenizing and mixing to obtain a solution.
The preparation method comprises the following steps:
(1) Heating, extinguishing, cracking and filtering the fermentation broth of the metabolic product adenine-9-D-anhydro-glycoside of streptomyces hygroscopicus obtained by fermentation, removing insoluble substances, and cooling aseptically for later use, wherein 4 parts of compound amino acid zinc, 10 parts of compound amino acid, 5 parts of nutrition enhancer, 5 parts of urea phosphate, 5 parts of potassium sulfate, 2 parts of choline chloride, 1 part of ketoglutaric acid, 1 part of compound vitamin, 10 parts of peptone and 7 parts of water are mixed and dissolved to obtain a solution A;
(2) And (3) adding 50 parts of fermentation liquor into the solution A obtained in the step (1), and mixing, homogenizing and stirring uniformly to obtain a product.
Example 3
Preparing a streptomyces hygroscopicus metabolite adenine-9-D-anhydro-amino-acid zinc type plant nutrient, weighing the following raw materials in parts by weight:
50 parts of streptomyces hygroscopicus LZ-17 strain metabolite fermentation liquor, 10 parts of compound amino acid zinc, 1 part of ketoglutaric acid, 1 part of compound vitamin, 5 parts of sugar, 1 part of trace element and 42 parts of water.
The preparation method comprises the following steps:
(1) Heating, extinguishing fire, cracking and filtering the fermentation broth of the metabolic product adenine-9-D-anhydro glucoside of the streptomyces hygroscopicus obtained by fermentation, removing insoluble substances, and cooling aseptically for later use, wherein 10 parts of compound amino acid zinc, 1 part of ketoglutaric acid, 1 part of compound vitamin, 5 parts of sugar, 1 part of trace element and 42 parts of water are dissolved to obtain a solution A;
(2) And adding 50 parts of fermentation liquor into the solution A obtained in the step, and uniformly mixing and stirring to obtain the product.
The embodiment and the comparative example of the invention are tested in 4 farms in Jiangsu, the test crops are cucumbers, grapes, strawberries and apple trees, the test fields are equal in parts, the soil components are the same, the number of the planted plants is the same, the facilities are the same in supply, the corresponding main components are selected for comparison, and the application mode is flushing application.
Effect example 1
Treatment 1: in example 1, the metabolite adenine-9-D-anhydro-amino acid zinc-type plant nutrient of streptomyces hygroscopicus is diluted into 600-fold solution and applied in the vigorous growth stage, the fruit inoculation stage and the melon bearing stage of cucumber respectively.
And 2, diluting the streptomyces hygroscopicus metabolite adenine-9-D-thioglycoside fermentation broth into 600-fold liquid, and performing flushing application in the vigorous growth stage, the fruit breeding stage and the melon bearing stage of the cucumber respectively.
And (3) treatment: the nutrient is prepared from 4 parts of compound amino acid zinc, 10 parts of peptone, 10 parts of secondary elements, 1 part of trace elements, 1 part of compound vitamins, 10 parts of sugar and 10 parts of amino acid, and is diluted into 600-fold liquid to be applied in the vigorous growth period, the fruit pregnancy period and the melon bearing period of the cucumbers respectively.
Treatment 4, diluting the metabolic product adenine-9-D-anhydro-amino-acid zinc type plant nutrient of the streptomyces hygroscopicus into 600-time solution, and respectively applying the solution in the vigorous growth stage, the fruit inoculation stage and the melon bearing stage of the cucumbers.
And (4) treatment 5: for comparison experiments, the cucumber extract is diluted into 600 times of solution and is applied in the vigorous growth stage, the fruit inoculation stage and the melon bearing stage of the cucumber respectively. The test results are shown in table 1.
TABLE 1 Effect of example 1 on cucumber yield and disease resistance
As can be seen from Table 1, the cucumber treated in example 1 has an increased yield and a reduced incidence of cucumber downy mildew and anthracnose compared to cucumbers treated with amino acids, nutrient supplement substances and trace elements and Streptomyces hygroscopicus metabolites alone. Compared with the treatments 1 and 4, the treatments 2 and 3 obviously improve the disease prevention effect and the yield on the premise of using the metabolic products of the streptomyces hygroscopicus and the zinc amino acid, the nutrition enhancer and the trace element nutrient substances independently in the same unit. Therefore, the effect of improving disease resistance and increasing yield of the regulatory plant nutrient is not generated by simple addition action, but generated by mutual synergistic action of the two.
compared with the treatment 1, the treatment 4 can prove that the experimental data are basically consistent, the repeatability is good, and the experimental parallelism reflecting experimental results are reliable.
Compared with treatments 1 and 4, the treatment 5 has the advantages that the yield of the cucumber treated by the comparison treatment 5 (blank experiment) is obviously reduced, the morbidity of the downy mildew and the anthracnose of the cucumber is increased, and the product has extremely high antibacterial effect and controls the occurrence of diseases in the experimental process.
effect example 2
Treatment 1: example 2, the metabolite adenine-9-D-anhydro-amino acid zinc-type plant nutrient of streptomyces hygroscopicus is diluted to 800 times of solution and is applied in dormancy stage, germination stage, leaf-expanding stage and fruit stage respectively.
And 2, diluting the fermentation liquor of the metabolic product adenine-9-D-anhydro-glycoside of the streptomyces hygroscopicus into 800-fold liquor, and respectively flushing and applying the liquor at the dormant stage, the sprouting stage, the leaf expanding stage and the fruit stage of the grapes.
And (3) treatment: the nutrient is prepared from 4 parts of compound amino acid zinc, 10 parts of peptone, 10 parts of secondary elements, 1 part of trace elements, 1 part of compound vitamins, 10 parts of sugar and 10 parts of amino acids, is diluted into 800-time liquid, and is applied by flushing in the dormant stage, the germination stage, the leaf expanding stage and the fruit stage of the grapes respectively.
And (4) treatment: in the embodiment, the metabolic product adenine-9-D-anhydro-amino acid zinc type plant nutrient of streptomyces hygroscopicus is diluted into 800 times of solution and is applied by flushing in the dormant stage, the germination stage, the leaf expanding stage and the fruit stage of grapes respectively.
And (4) treatment 5: in contrast experiments, the grape wine is diluted into 800 times of liquid and is applied in the dormancy stage, the germination stage, the leaf expanding stage and the fruit stage of the grape respectively. The test results are shown in table 2.
TABLE 2 Effect of example 2 on grape production and disease resistance
As can be seen from Table 2, the grapes treated in example 2 showed an increased yield and a reduced incidence of downy and brown spot of grapes compared to grapes treated with amino acids, nutrient supplement substances and trace elements and metabolites of Streptomyces hygroscopicus alone. Compared with the treatments 1 and 4, the treatments 2 and 3 obviously improve the disease prevention effect and the yield on the premise of using the metabolic products of the streptomyces hygroscopicus and the zinc amino acid, the nutrition enhancer and the trace element nutrient substances independently in the same unit. Therefore, the effect of improving disease resistance and increasing yield of the regulatory plant nutrient is not generated by simple addition action, but generated by mutual synergistic action of the two.
Compared with the treatment 1, the treatment 4 can prove that the experimental data are basically consistent, the repeatability is good, and the experimental parallelism reflecting experimental results are reliable.
Compared with treatments 1 and 4, the treatment 5 has the advantages that the yield of the grapes treated by the comparison treatment 5 (blank experiment) is obviously reduced, the morbidity of downy mildew and brown spot of the grapes is increased, and the product has extremely high antibacterial effect and controls the occurrence of diseases in the experiment process.
Effect example 3
Treatment 1: example 3, the metabolite adenine-9-D-anhydro-amino acid zinc-type plant nutrient of streptomyces hygroscopicus is diluted into 800 times of solution, and is applied by flushing in the resting stage, the germination stage, the leaf expanding stage and the fruit stage of the soybeans respectively.
And 3, diluting the fermentation liquor of the metabolic product adenine-9-D-angou glucoside of the streptomyces hygroscopicus into 800 times of liquor, and respectively applying the liquor in the germination stage, the leaf expanding stage and the pod setting stage of the soybeans.
And (3) treatment: the nutrient agent is prepared from 4 parts of compound zinc amino acid, 10 parts of peptone, 10 parts of secondary elements, 1 part of trace elements, 1 part of compound vitamins, 10 parts of sugar and 10 parts of amino acid, is diluted into 800 times of liquid, and is applied by flushing in the germination stage, the leaf expanding stage and the pod setting stage of the soybeans respectively.
And (4) treatment: in the embodiment, the metabolic product adenine-9-D-anhydro-amino acid zinc type plant nutrient of streptomyces hygroscopicus is diluted into 800 times of solution and is applied by flushing in the germination stage, the leaf expanding stage and the pod setting stage of soybeans respectively.
And (4) treatment 5: in contrast experiments, the extract is diluted with water to 800 times and applied in the germination stage, the leaf expanding stage and the pod setting stage of the soybeans respectively. The test results are shown in table 3.
TABLE 3 Effect of example 3 on Soybean yield and disease resistance
As can be seen from Table 3, the soybeans treated in example 3 showed increased yield and reduced incidence of downy and root mildew of soybeans compared to soybeans treated with amino acids, dietary supplement substances and trace elements alone and metabolites of Streptomyces hygroscopicus. Compared with the treatments 1 and 4, the treatments 2 and 3 obviously improve the disease prevention effect and the yield on the premise of using the metabolic products of the streptomyces hygroscopicus and the zinc amino acid, the nutrition enhancer and the trace element nutrient substances independently in the same unit. Therefore, the effect of improving disease resistance and increasing yield of the regulatory plant nutrient is not generated by simple addition action, but generated by mutual synergistic action of the two.
Compared with the treatment 1, the treatment 4 can prove that the experimental data are basically consistent, the repeatability is good, and the experimental parallelism reflecting experimental results are reliable.
Compared with the treatments 1 and 4, the treatment 5 has the advantages that the yield of the soybeans is obviously reduced through the comparison treatment 5 (blank experiment), the incidence rates of the downy mildew and the root mildew of the soybeans are increased, and the product has extremely high antibacterial effect and controls the occurrence of diseases in the experimental process.
Effect example 4
treatment 1: example 4, the metabolite adenine-9-D-anhydro-amino acid zinc-type plant nutrient of streptomyces hygroscopicus is diluted into 600-fold solution and applied in the pre-flowering stage and the post-flowering stage of apple trees by flushing.
And 2, diluting the Streptomyces hygroscopicus metabolite adenine-9-D-thioglycoside fermentation broth into 600-fold liquid, and performing flushing application on the apple trees in the pre-flowering stage and the post-flowering stage respectively.
And (3) treatment: the nutrient agent is prepared from 4 parts of compound amino acid zinc, 10 parts of peptone, 10 parts of secondary elements, 1 part of trace elements, 1 part of compound vitamins, 10 parts of sugar and 10 parts of amino acid, is diluted into 800-time liquid, and is applied by flushing in the pre-flowering and post-flowering stages of the apple respectively.
And 4, diluting the metabolic product adenine-9-D-anhydro-amino-acid zinc type plant nutrient of the streptomyces hygroscopicus into 600-time solution, and performing flushing application on the plant nutrient in the pre-flowering stage and the post-flowering stage of the apple trees respectively.
And (4) treatment 5: in contrast experiments, the solution is diluted by water to 600 times and is applied in the fertilizing period before the flowering and in the post-flowering period of the apple trees respectively. The test results are shown in table 4.
TABLE 4 Effect of example 4 on apple yield and disease resistance
As can be seen from Table 4, the apples treated in example 4 have increased yield and reduced incidence of apple rot and alternaria leaf spot compared to apples treated with amino acids, nutrient supplement substances and trace elements and metabolites of Streptomyces hygroscopicus alone. Compared with the treatments 1 and 4, the treatments 2 and 3 obviously improve the disease prevention effect and the yield on the premise of using the metabolic products of the streptomyces hygroscopicus and the zinc amino acid, the nutrition enhancer and the trace element nutrient substances independently in the same unit. Therefore, the effect of improving disease resistance and increasing yield of the regulatory plant nutrient is not generated by simple addition action, but generated by mutual synergistic action of the two.
Compared with the treatment 1, the treatment 4 can prove that the experimental data are basically consistent, the repeatability is good, and the experimental parallelism reflecting experimental results are reliable.
Compared with the treatments 1 and 4, the treatment 5 has the advantages that the yield of the apples is obviously reduced through the comparison treatment 5 (blank experiment), the incidence rates of apple canker and alternaria leaf spot are increased, and the product has extremely high antibacterial effect and controls the occurrence of diseases in the experimental process.
effect example 5
Treatment 1: example 4, the metabolite adenine-9-D-anhydro-amino acid zinc-type plant nutrient of streptomyces hygroscopicus is diluted into 600-fold solution, and is applied in the early fertile period and the late flowering period of strawberry respectively.
and 2, diluting the fermentation liquor of the metabolic product adenine-9-D-anhydrobiose of the streptomyces hygroscopicus into 600-fold liquor, and performing flushing application on the strawberry in the early fattening period and the late flowering period respectively.
And (3) treatment: the nutrient is prepared from 4 parts of compound amino acid zinc, 10 parts of peptone, 10 parts of secondary elements, 1 part of trace elements, 1 part of compound vitamins, 10 parts of sugar and 10 parts of amino acid, is diluted into 800-time liquid, and is applied in early stage and late stage of strawberry flowering respectively.
And 4, diluting the metabolic product adenine-9-D-anhydro-amino-acid zinc type plant nutrient of the streptomyces hygroscopicus into 600-time solution, and performing flushing application on the early stage and the late stage of the strawberry blossom respectively.
And (4) treatment 5: for a control experiment, the solution is diluted by 600 times with water and is applied in the early stage and the later stage of the strawberry flowering respectively.
TABLE 5 Effect of example 5 on strawberry yield and disease resistance
As can be seen from Table 5, the strawberries treated in example 5 showed an increased yield and reduced incidence of powdery mildew and gray mold in comparison to strawberries treated with amino acids, nutrient supplement substances and trace elements and metabolites of Streptomyces hygroscopicus alone. Compared with the treatments 1 and 4, the treatments 2 and 3 obviously improve the disease prevention effect and the yield on the premise of using the metabolic products of the streptomyces hygroscopicus and the zinc amino acid, the nutrition enhancer and the trace element nutrient substances independently in the same unit. Therefore, the effect of improving disease resistance and increasing yield of the regulatory plant nutrient is not generated by simple addition action, but generated by mutual synergistic action of the two.
Compared with the treatment 1, the treatment 4 can prove that the experimental data are basically consistent, the repeatability is good, and the experimental parallelism reflecting experimental results are reliable.
Compared with the treatments 1 and 4, the treatment 5 has the advantages that the yield of the strawberries is obviously reduced through the comparison treatment 5 (blank experiment), the morbidity of powdery mildew and gray mold of the strawberries is increased, and the product has extremely high antibacterial effect and controls the occurrence of diseases in the experiment process.
In conclusion, the regulating plant nutrient has obvious disease-resistant and yield-increasing effects on crops, and the main components of the regulating plant nutrient, namely the zinc amino acid, the nutrition enhancer, the trace elements and the metabolic products of the streptomyces hygroscopicus, have the effects of being matched and used in a certain proportion to generate the synergistic effect. By further optimizing the ratio of nutrient substances to trace elements, a better synergistic effect can be generated.
While particular embodiments of the present invention have been illustrated and described, it would be obvious that various other changes and modifications can be made without departing from the spirit and scope of the invention. It is therefore intended to cover in the appended claims all such changes and modifications that are within the scope of this invention.
Claims (10)
1. A compound amino acid zinc type plant nutrient prepared by using streptomyces hygroscopicus metabolite adenine-9-D-anhydro-glucoside fermentation liquor is characterized by mainly being prepared from the following raw materials in parts by weight:
1-90 parts of streptomyces hygroscopicus metabolite adenine-9-D-anhydrobiose fermentation liquor,
4-70 parts of compound amino acid zinc,
1-20 parts of peptone,
10-90 parts of water, namely,
Mixing the above materials, sterilizing at 60 ~ 70 deg.C, and cooling to obtain mixed solution.
2. the amino acid zinc type plant nutrient compounded by the streptomyces hygroscopicus metabolite adenine-9-D-angou glycoside fermentation liquid according to claim 1, which is characterized in that: the raw materials also comprise 1-20 parts by weight of compound amino acid and 1-20 parts by weight of nutrition enhancer.
3. The amino acid zinc type plant nutrient compounded by the streptomyces hygroscopicus metabolite adenine-9-D-anhydro-glycoside fermentation liquid according to claim 1 or 2, which is characterized in that: the raw materials also comprise 1-10 parts of secondary elements and 1-10 parts of trace elements.
4. The amino acid zinc type plant nutrient compounded by the streptomyces hygroscopicus metabolite adenine-9-D-angou glycoside fermentation liquid according to claim 1, which is characterized in that: the amino acid zinc is one or the combination of more than two of zinc glycinate, zinc lysinate, zinc tryptophan, zinc proline, zinc methionine, zinc glutamate, zinc alanine and zinc leucine, and the total weight part of the amino acid zinc is not less than 2%.
5. The amino acid zinc type plant nutrient compounded by the streptomyces hygroscopicus metabolite adenine-9-D-angou glycoside fermentation liquid according to claim 2, which is characterized in that: the compound amino acid is one or the combination of more than two of glycine, alanine, valine, leucine, isoleucine, phenylalanine, tryptophan, tyrosine, aspartic acid, histidine, asparagine, glutamic acid, lysine, glutamine, methionine, arginine, serine, threonine, cysteine and proline.
6. The amino acid zinc type plant nutrient compounded by the streptomyces hygroscopicus metabolite adenine-9-D-angou glycoside fermentation liquid according to claim 2, which is characterized in that: the nutrition enhancer is one or the combination of more than two of vitamin B1, vitamin B2, vitamin B3, vitamin B4, vitamin B5, vitamin B6, vitamin B7, vitamin B11, vitamin B12, vitamin C, vitamin D, vitamin E, vitamin F, vitamin K, vitamin P, vitamin T, vitamin U, vitamin A, inositol, choline chloride, ketoglutaric acid, melatonin, sugar alcohol calcium, polyamino acids, animal small peptides, oligosaccharide, chitosan, nucleotide, magnesium sulfate, ferrous sulfate, ferric amino acid, copper sulfate, copper amino acid, manganese sulfate, manganese amino acid, zinc sulfate and potassium silicate.
7. The amino acid zinc type plant nutrient compounded by the streptomyces hygroscopicus metabolite adenine-9-D-angou glycoside fermentation liquid according to claim 3, which is characterized in that: the trace elements comprise one or more of iodine, boron, cobalt, molybdenum, titanium, lanthanum compounds or organic matters.
8. the amino acid zinc type plant nutrient compounded by the streptomyces hygroscopicus metabolite adenine-9-D-angou glycoside fermentation liquid according to claim 1, which is characterized in that: the fermentation liquor of the metabolite adenine-9-D-angou glucoside of the streptomyces hygroscopicus is fermentation liquor obtained by taking a strain of streptomyces hygroscopicus LZ-17 as a starting strain to obtain the metabolite adenine-9-D-angou glucoside, and fermenting the strain of streptomyces hygroscopicus LZ-17 after completely metabolizing the strain.
9. The compound amino acid zinc type plant nutrient with the streptomyces hygroscopicus metabolite adenine-9-D anhydro glycoside fermentation broth as claimed in claim 1, is characterized in that the streptomyces hygroscopicus LZ-17 strain is activated and then inoculated into a glucose asparagine culture medium, wherein the culture medium comprises 8 g of glucose 8 ~ 12, 0.5 g of asparagine 0.5 ~ 1g, 0.5 g of dipotassium phosphate 0.5 ~ 1g, 150 ~ 250mg/L of threonine and 1000mL of distilled water in a 2000L fermentation tank, and the threonine is fed with feed for fermentation for 72 hours.
10. The compound amino acid zinc type plant nutrient with the streptomyces hygroscopicus metabolite adenine-9-D-angou glycoside fermentation liquid as claimed in claim 1 is characterized in that the streptomyces hygroscopicus metabolite adenine-9-D-angou glycoside fermentation liquid needs to be inactivated and cracked before being homogenized and mixed, specifically, the streptomyces hygroscopicus metabolite adenine-9-D-angou glycoside fermentation liquid is heated to 65 ~ 100 ℃ for inactivation and cracking, and is subjected to nano filter element filtration to remove insoluble substances and then is subjected to aseptic cooling.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910933607.8A CN110577441A (en) | 2019-09-29 | 2019-09-29 | Amino acid zinc type plant nutrient compounded by streptomyces hygroscopicus metabolite adenine-9-D-anhydro glucoside fermentation liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910933607.8A CN110577441A (en) | 2019-09-29 | 2019-09-29 | Amino acid zinc type plant nutrient compounded by streptomyces hygroscopicus metabolite adenine-9-D-anhydro glucoside fermentation liquid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110577441A true CN110577441A (en) | 2019-12-17 |
Family
ID=68813864
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910933607.8A Pending CN110577441A (en) | 2019-09-29 | 2019-09-29 | Amino acid zinc type plant nutrient compounded by streptomyces hygroscopicus metabolite adenine-9-D-anhydro glucoside fermentation liquid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110577441A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113277954A (en) * | 2021-06-17 | 2021-08-20 | 英德市匠心新材料股份有限公司 | Amino acid metal chelate micro-nano powder and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104099281A (en) * | 2014-07-20 | 2014-10-15 | 尹梦珍 | Environment-friendly compound microbial agent |
| CN106518393A (en) * | 2016-11-10 | 2017-03-22 | 辽宁微科生物工程股份有限公司 | Major-element liquid fertilizer containing streptomyces ahygroscopicus metabolites and preparation method of major-element liquid fertilizer |
| CN108046917A (en) * | 2017-12-22 | 2018-05-18 | 北京都丰年生态农业科技有限公司 | High efficiency nutritious dose and its preparation and application method in a kind of plantation for fruits and vegetables |
| CN108558498A (en) * | 2018-04-04 | 2018-09-21 | 合肥市风达农业有限责任公司 | A kind of stalk organic environmental-protection fertilizer and preparation method thereof |
| CN109776236A (en) * | 2018-12-26 | 2019-05-21 | 龙蟒大地农业有限公司 | A kind of preparation method improveing acidic soil conditioner |
-
2019
- 2019-09-29 CN CN201910933607.8A patent/CN110577441A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104099281A (en) * | 2014-07-20 | 2014-10-15 | 尹梦珍 | Environment-friendly compound microbial agent |
| CN106518393A (en) * | 2016-11-10 | 2017-03-22 | 辽宁微科生物工程股份有限公司 | Major-element liquid fertilizer containing streptomyces ahygroscopicus metabolites and preparation method of major-element liquid fertilizer |
| CN108046917A (en) * | 2017-12-22 | 2018-05-18 | 北京都丰年生态农业科技有限公司 | High efficiency nutritious dose and its preparation and application method in a kind of plantation for fruits and vegetables |
| CN108558498A (en) * | 2018-04-04 | 2018-09-21 | 合肥市风达农业有限责任公司 | A kind of stalk organic environmental-protection fertilizer and preparation method thereof |
| CN109776236A (en) * | 2018-12-26 | 2019-05-21 | 龙蟒大地农业有限公司 | A kind of preparation method improveing acidic soil conditioner |
Non-Patent Citations (2)
| Title |
|---|
| 嵇汝运，张天禄主编；中国药学会《药学大辞典》编辑委员会编: "《药学大辞典》", 30 September 2006 * |
| 王运兵主编: "《无公害农药实用手册 第2版》", 30 September 2010 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113277954A (en) * | 2021-06-17 | 2021-08-20 | 英德市匠心新材料股份有限公司 | Amino acid metal chelate micro-nano powder and preparation method thereof |
| CN113277954B (en) * | 2021-06-17 | 2023-04-28 | 英德市匠心新材料股份有限公司 | Amino acid metal chelate micro-nano powder and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105039210A (en) | Complex lively bacterial preparation as well as water soluble fertilizer and application thereof | |
| CN107759262A (en) | A kind of fertilizing method of high yield high nutrition quality red bayberry | |
| CN108623344A (en) | A kind of organic fertilizer and its preparation method and application | |
| CN100535104C (en) | Method for preparing feedstuff yeast from maize peel hydrolysis solution | |
| CN115094107A (en) | Method for preparing soybean polypeptide composition and polypeptide amino acid fertilizer and product | |
| CN113620730B (en) | Organic fertilizer formula for roses | |
| CN106518527A (en) | Biological fertilizer prepared from tea leaves and preparation method of biological fertilizer | |
| CN109836298A (en) | A kind of biological humic acid ferment liquid fertilizer and the preparation method and application thereof | |
| CN110577441A (en) | Amino acid zinc type plant nutrient compounded by streptomyces hygroscopicus metabolite adenine-9-D-anhydro glucoside fermentation liquid | |
| CN108821858A (en) | The disease-resistant dedicated bacterial manure of dragon fruit, preparation method and applications | |
| CN107500816A (en) | A kind of method for preparing organic fertilizer using mango discarded object fermentation | |
| CN109438079B (en) | Soluble biological organic fertilizer and production method thereof | |
| CN107974423B (en) | Soil biological activator and preparation method thereof | |
| CN110981638A (en) | Biological organic fertilizer with saline-alkali soil improvement effect and preparation process thereof | |
| CN110606781A (en) | Full-water-soluble organic-inorganic compound fertilizer and preparation method thereof | |
| CN108101707B (en) | Total nutrient microbial amino acid water-soluble fertilizer and preparation method thereof | |
| CN108440148A (en) | A kind of preparation method of high-efficiency foliage fertilizer | |
| CN109095994A (en) | A kind of biogas slurry humic acid type liquid organic fertilizer and its preparation process | |
| JPH02199091A (en) | Production of liquid fertilizer | |
| CN114751784A (en) | Composite biological selenium-rich foliar fertilizer and preparation method thereof | |
| CN109400410B (en) | Method for producing biological organic matter by using threonine fermentation waste liquid | |
| CN111943438A (en) | Biochemical treatment method for animal harmless treatment wastewater, nutrient solution prepared by biochemical treatment method and application of nutrient solution | |
| CN113387742A (en) | Nano organic selenium fertilizer and preparation method and application thereof | |
| CN112979383A (en) | Bio-organic fertilizer for promoting crop growth and preparation method and application thereof | |
| CN106631446B (en) | Organic master batch and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20191217 |