CN110564809A - rotary streaking inoculation method - Google Patents
rotary streaking inoculation method Download PDFInfo
- Publication number
- CN110564809A CN110564809A CN201910880691.1A CN201910880691A CN110564809A CN 110564809 A CN110564809 A CN 110564809A CN 201910880691 A CN201910880691 A CN 201910880691A CN 110564809 A CN110564809 A CN 110564809A
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- Prior art keywords
- culture dish
- inoculator
- streaking
- inoculation
- rotary
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- 238000011081 inoculation Methods 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title claims abstract description 18
- 239000001963 growth medium Substances 0.000 claims abstract description 10
- 239000007788 liquid Substances 0.000 claims abstract description 9
- 239000000523 sample Substances 0.000 description 6
- 238000000576 coating method Methods 0.000 description 3
- 238000001514 detection method Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 235000009161 Espostoa lanata Nutrition 0.000 description 1
- 240000001624 Espostoa lanata Species 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to a rotary streak inoculation method, which comprises the following steps that a, a culture dish containing a culture medium rotates; b. horizontally moving the inoculator dipped with the sample liquid along the radius direction of the culture dish, and carrying out inoculation and streaking on a culture medium on the culture dish; c. the inoculator is rotated about an axis. According to the rotary streak inoculation method, an inoculator dipped with sample liquid horizontally moves along the radius direction of a culture dish, and inoculation streaking is carried out on a culture medium on the culture dish; the inoculator is rotated about an axis. The spiral streak inoculation is realized, and the inoculation is more uniform.
Description
Technical Field
the invention relates to a culture dish coating device, in particular to a device for dynamically streaking a rotary inoculator on a rotary culture dish.
Background
In hospital clinical and medical research trials, it is often necessary to spread biological samples or prepared bacterial suspensions from patients onto petri dishes. The current general coating method: the coating process is completed by using a traditional inoculating loop and adopting a manual method.
however, due to the existing manual streaking inoculation mode, the randomness is high in the streaking process, and the liquid sample is difficult to be uniformly coated on the culture dish according to the assumption of a tester, so that the detection result is influenced.
Disclosure of Invention
In order to solve the problems, the invention provides a rotary streak inoculation method which is reasonable in structure and convenient to use.
The technical scheme adopted by the invention is as follows: a method of rotational streaking inoculation, comprising: comprises the following steps of (a) carrying out,
a. Rotating the culture dish containing the culture medium;
b. The inoculator dipped with the sample liquid horizontally moves along the radius direction of the culture dish, or from the circle center to the circle center;
c. the inoculator was rotated about the axis and self-transferred 360 deg. from start to end of the scoring process.
the three movements start and end simultaneously.
preferably, the culture dish rotates according to a set speed. Meanwhile, the inoculator is automatically transferred along the shaft and moves along the radius direction of the culture dish at the same time.
Preferably, the tail end of the inoculator is dipped in the sample liquid, then contacts with the culture dish, moves along the radius direction of the culture dish while automatically transmitting around the shaft, and carries out dynamic inoculation and streaking.
The beneficial effects obtained by the invention are as follows: according to the rotary streak inoculation method, an inoculator dipped with sample liquid horizontally moves along the radius direction of a culture dish, and inoculation and streak are carried out on the rotary culture dish; simultaneously the inoculator was rotated about the axis, i.e. the whole of the sample carried on the inoculator was streaked 360 ° a week onto the culture medium in a petri dish. The spiral streak inoculation is realized, and the inoculation is more uniform and controllable.
drawings
FIG. 1 is a prior art inoculation streaking method;
FIG. 2 is a streaking inoculation mode of the present invention;
FIG. 3 is a schematic diagram of detection by drug sensitive test paper;
Reference numerals: 1. a culture dish; 2. an inoculator; 3. drug sensitive test paper.
Detailed Description
The invention will be further described with reference to the following drawings and specific embodiments.
As shown in FIGS. 1-3, a spin-streaking method of the present invention is characterized by: comprises the following steps of (a) carrying out,
a. The culture dish 1 containing the culture medium rotates according to a set speed;
b. Horizontally moving the inoculator 2 dipped with the sample liquid along the radius direction of the culture dish, and carrying out inoculation and streaking on the culture medium on the culture dish 1;
c. The inoculator 2 rotates around an axis, and the inoculator 2 automatically transfers around the axis while moving along the radius of the culture dish, so that all samples on the inoculator can be conveniently coated on the culture dish in 360 degrees.
By the streaking inoculation method, the inoculator 2 can perform spiral streaking on the culture medium, and streaking inoculation is ensured to be more uniform.
In this example, the sample solution was dipped into the end of the inoculator 2 by a cotton ball or sponge, and then streaked.
the foregoing shows and describes the general principles and principal structural features of the present invention. The present invention is not limited to the above examples, and various changes and modifications may be made without departing from the spirit and scope of the invention, which fall within the scope of the claimed invention. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (5)
1. A method of rotational streaking inoculation, comprising: comprises the following steps of (a) carrying out,
a. rotating the culture dish containing the culture medium;
b. Horizontally moving the inoculator dipped with the sample liquid along the radius direction of the culture dish, and carrying out inoculation and streaking on a culture medium on the culture dish;
c. The inoculator is rotated about an axis.
2. The rotational streaking inoculation method of claim 1 wherein: the culture dish is rotated according to a set speed.
3. A method of rotational streaking as in claim 1 or claim 2 wherein: the inoculator pivoting is performed simultaneously with the rotation of the culture dish.
4. The rotational streaking inoculation method of claim 1 wherein: the inoculator moves horizontally along the radius direction of the culture dish.
5. the rotational streaking inoculation method of claim 1 wherein: the end of the inoculator is dipped with the sample liquid, and then inoculation streaking is carried out on a culture dish.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910880691.1A CN110564809A (en) | 2019-09-18 | 2019-09-18 | rotary streaking inoculation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910880691.1A CN110564809A (en) | 2019-09-18 | 2019-09-18 | rotary streaking inoculation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110564809A true CN110564809A (en) | 2019-12-13 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910880691.1A Pending CN110564809A (en) | 2019-09-18 | 2019-09-18 | rotary streaking inoculation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110564809A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117683612A (en) * | 2023-12-15 | 2024-03-12 | 佳木斯大学 | Microorganism separation culture apparatus |
Citations (9)
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|---|---|---|---|---|
| FR2876385A1 (en) * | 2004-10-12 | 2006-04-14 | Interscience Sarl | Inoculator for depositing biological solutions in a culture medium comprises distribution of the solution by using an inoculating arm with a tubular element/stylet that delivers the solution above the medium recipient |
| CN101892149A (en) * | 2009-06-02 | 2010-11-24 | 英特莱波公司 | System for supplying an automatic inoculating device and process using such an automatic device |
| CN103173392A (en) * | 2013-03-19 | 2013-06-26 | 云南省烟草农业科学研究院 | Method for magnetically and rotatably applying bacteria on culture medium panel |
| CN104152343A (en) * | 2014-07-16 | 2014-11-19 | 北京赛智创业科技有限公司 | Spin coating device |
| CN204125457U (en) * | 2014-10-30 | 2015-01-28 | 邹叶青 | A kind of quick bacteria spreader |
| US20150353983A1 (en) * | 2013-01-21 | 2015-12-10 | bioMérieux | Means, method and computer program product for determining the concentration level of microorganisms during a fluid analysis |
| CN205188267U (en) * | 2015-12-04 | 2016-04-27 | 济南百博生物技术股份有限公司 | Placing of culture dish, fixed and rotary device |
| CN105695556A (en) * | 2016-02-18 | 2016-06-22 | 深圳市依波特科技有限公司 | Screw inoculation method |
| CN105886383A (en) * | 2016-06-14 | 2016-08-24 | 重庆庞通医疗器械有限公司 | Microorganism streak inoculation instrument |
-
2019
- 2019-09-18 CN CN201910880691.1A patent/CN110564809A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2876385A1 (en) * | 2004-10-12 | 2006-04-14 | Interscience Sarl | Inoculator for depositing biological solutions in a culture medium comprises distribution of the solution by using an inoculating arm with a tubular element/stylet that delivers the solution above the medium recipient |
| CN101892149A (en) * | 2009-06-02 | 2010-11-24 | 英特莱波公司 | System for supplying an automatic inoculating device and process using such an automatic device |
| US20150353983A1 (en) * | 2013-01-21 | 2015-12-10 | bioMérieux | Means, method and computer program product for determining the concentration level of microorganisms during a fluid analysis |
| CN103173392A (en) * | 2013-03-19 | 2013-06-26 | 云南省烟草农业科学研究院 | Method for magnetically and rotatably applying bacteria on culture medium panel |
| CN104152343A (en) * | 2014-07-16 | 2014-11-19 | 北京赛智创业科技有限公司 | Spin coating device |
| CN204125457U (en) * | 2014-10-30 | 2015-01-28 | 邹叶青 | A kind of quick bacteria spreader |
| CN205188267U (en) * | 2015-12-04 | 2016-04-27 | 济南百博生物技术股份有限公司 | Placing of culture dish, fixed and rotary device |
| CN105695556A (en) * | 2016-02-18 | 2016-06-22 | 深圳市依波特科技有限公司 | Screw inoculation method |
| CN105886383A (en) * | 2016-06-14 | 2016-08-24 | 重庆庞通医疗器械有限公司 | Microorganism streak inoculation instrument |
Non-Patent Citations (2)
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| 中华人民共和国国家质量监督检验检疫总局: "食品和化妆品中的菌落技术检测方法 螺旋平板法", 《SN/T 2098-2008》 * |
| 黄维雅: "食品中菌落总数的国标法测定注意事项及快速检测技术", 《台湾农业探索》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117683612A (en) * | 2023-12-15 | 2024-03-12 | 佳木斯大学 | Microorganism separation culture apparatus |
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| PB01 | Publication | ||
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| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20191213 |
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| RJ01 | Rejection of invention patent application after publication |