CN110558938A - Parathyroid gland recognition device and system - Google Patents
Parathyroid gland recognition device and system Download PDFInfo
- Publication number
- CN110558938A CN110558938A CN201910771976.1A CN201910771976A CN110558938A CN 110558938 A CN110558938 A CN 110558938A CN 201910771976 A CN201910771976 A CN 201910771976A CN 110558938 A CN110558938 A CN 110558938A
- Authority
- CN
- China
- Prior art keywords
- light source
- parathyroid
- light
- autofluorescence
- parathyroid gland
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000002990 parathyroid gland Anatomy 0.000 title claims abstract description 154
- 238000012545 processing Methods 0.000 claims abstract description 31
- 238000001914 filtration Methods 0.000 claims abstract description 25
- 238000000034 method Methods 0.000 claims abstract description 21
- 238000001514 detection method Methods 0.000 claims abstract description 18
- 230000005284 excitation Effects 0.000 claims description 73
- 239000013307 optical fiber Substances 0.000 claims description 42
- 230000000849 parathyroid Effects 0.000 claims description 39
- 239000000523 sample Substances 0.000 claims description 38
- 238000003384 imaging method Methods 0.000 claims description 34
- 230000003287 optical effect Effects 0.000 claims description 23
- 238000006243 chemical reaction Methods 0.000 claims description 19
- 206010052428 Wound Diseases 0.000 claims description 16
- 208000027418 Wounds and injury Diseases 0.000 claims description 16
- 238000010183 spectrum analysis Methods 0.000 claims description 14
- 239000000835 fiber Substances 0.000 claims description 12
- 239000010453 quartz Substances 0.000 claims description 12
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 12
- 230000005540 biological transmission Effects 0.000 claims description 11
- 239000013308 plastic optical fiber Substances 0.000 claims description 11
- 238000001228 spectrum Methods 0.000 claims description 9
- 238000004891 communication Methods 0.000 claims description 8
- 238000012360 testing method Methods 0.000 claims description 5
- 208000002847 Surgical Wound Diseases 0.000 claims description 3
- 229910052736 halogen Inorganic materials 0.000 claims description 2
- 150000002367 halogens Chemical class 0.000 claims description 2
- 239000004065 semiconductor Substances 0.000 claims description 2
- 229910052724 xenon Inorganic materials 0.000 claims description 2
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 claims description 2
- 230000001276 controlling effect Effects 0.000 claims 2
- 230000008878 coupling Effects 0.000 claims 1
- 238000010168 coupling process Methods 0.000 claims 1
- 238000005859 coupling reaction Methods 0.000 claims 1
- 230000001939 inductive effect Effects 0.000 claims 1
- 230000001105 regulatory effect Effects 0.000 claims 1
- 208000000038 Hypoparathyroidism Diseases 0.000 abstract description 7
- 238000005516 engineering process Methods 0.000 abstract description 5
- 206010053260 Secondary hyperthyroidism Diseases 0.000 abstract description 4
- 210000001519 tissue Anatomy 0.000 description 27
- 238000010586 diagram Methods 0.000 description 18
- 229960000907 methylthioninium chloride Drugs 0.000 description 5
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 description 4
- 238000002189 fluorescence spectrum Methods 0.000 description 4
- MOFVSTNWEDAEEK-UHFFFAOYSA-M indocyanine green Chemical compound [Na+].[O-]S(=O)(=O)CCCCN1C2=CC=C3C=CC=CC3=C2C(C)(C)C1=CC=CC=CC=CC1=[N+](CCCCS([O-])(=O)=O)C2=CC=C(C=CC=C3)C3=C2C1(C)C MOFVSTNWEDAEEK-UHFFFAOYSA-M 0.000 description 4
- 229960004657 indocyanine green Drugs 0.000 description 4
- 230000035515 penetration Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 229940079593 drug Drugs 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 230000002452 interceptive effect Effects 0.000 description 3
- 230000001678 irradiating effect Effects 0.000 description 3
- 230000003595 spectral effect Effects 0.000 description 3
- 230000017531 blood circulation Effects 0.000 description 2
- 230000036770 blood supply Effects 0.000 description 2
- 238000004141 dimensional analysis Methods 0.000 description 2
- 230000031700 light absorption Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 210000003205 muscle Anatomy 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 230000002093 peripheral effect Effects 0.000 description 2
- 108091008695 photoreceptors Proteins 0.000 description 2
- 238000001356 surgical procedure Methods 0.000 description 2
- 210000001685 thyroid gland Anatomy 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 238000002073 fluorescence micrograph Methods 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000017525 heat dissipation Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 229910021392 nanocarbon Inorganic materials 0.000 description 1
- 230000005693 optoelectronics Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 210000003437 trachea Anatomy 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0071—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by measuring fluorescence emission
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0075—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by spectroscopy, i.e. measuring spectra, e.g. Raman spectroscopy, infrared absorption spectroscopy
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/42—Detecting, measuring or recording for evaluating the gastrointestinal, the endocrine or the exocrine systems
- A61B5/4222—Evaluating particular parts, e.g. particular organs
- A61B5/4227—Evaluating particular parts, e.g. particular organs endocrine glands, i.e. thyroid, adrenals, hypothalamic, pituitary
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/48—Other medical applications
- A61B5/4887—Locating particular structures in or on the body
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- Surgery (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Heart & Thoracic Surgery (AREA)
- Medical Informatics (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Animal Behavior & Ethology (AREA)
- Pathology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Urology & Nephrology (AREA)
- Endocrinology (AREA)
- Gastroenterology & Hepatology (AREA)
- Physiology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
A parathyroid gland recognition device and system, in the field of biological tissue detection, identification technology, include the autofluorescence inspiring device, is used for producing the exciting light of 650-810nm, illuminate the human tissue, make the parathyroid gland produce the autofluorescence; and an autofluorescence collecting and processing device for collecting and processing the autofluorescence of parathyroid gland into identifiable information; the parathyroid gland identification device is characterized by further comprising a light filtering device, wherein the light filtering device penetrates through the light source with the wavelength of 810nm, so that interference light of the light source cannot penetrate through the light filtering device, and parathyroid gland identification is prevented from being interfered. The invention is applied to each link of the operation by matching various identification devices, has large identification range, high speed and accuracy, and reduces the incidence rate of permanent hypoparathyroidism to the maximum extent. The invention corresponds to a patient with secondary hyperthyroidism, the parathyroid gland needs to be resected in the process, and the invention can identify and resect the secondary hyperthyroidism which is difficult to find by a doctor.
Description
The present invention claims domestic priority, referred to as priority A, B, C, respectively, of the following patent applications:
Priority A: the invention has the application number of '201921039946.3', the application date of '7/4/2019', and the invention and creation name of 'a laser probe for fluorescence detection'.
Priority B: the invention relates to a handheld human body autofluorescence detection device, which has the application number of 201910269417.0, the application date of which is 4 months and 4 days in 2019.
Priority C: the invention has the application number of '201910582533.8', the application date of '2019, 6 and 29 days', and the invention and creation name of the invention is 'a surface imaging fluorescence spectrum analysis system'.
Technical Field
The invention relates to a device and a system for identifying parathyroid gland, belonging to the technical field of biological tissue detection and identification.
Background
hypoparathyroidism is an important complication of thyroidectomy. After the thyroidectomy, the incidence rate of temporary hypoparathyroidism is reported to be 20% -60%, and the incidence rate of permanent hypoparathyroidism is reported to be 1% -7%, so that the effective preservation of parathyroid gland in the operation is very important, and accurate identification of parathyroid gland in the operation is a precondition for effectively preserving parathyroid gland.
In general, parathyroid glands have the following characteristics: 1) and (2) micro: the size is about several millimeters, and the size is extremely difficult to be found by naked eyes; 2) difficult discernment: is difficult to distinguish from normal tissues such as fat granules and the like; 3) the number is not fixed: the number of parathyroid glands is generally 3-5, and about 48% -62% of Chinese people have 4 parathyroid glands according to literature reports; 4) the position is not fixed: parathyroid gland location varies from person to person; 5) can be planted within a specific time.
in the prior art, there are several methods for identifying parathyroid gland:
1, intraoperative macroscopic identification: identifying through naked eyes directly or by means of cavity mirror;
2, dyeing identification: parathyroid glands are labeled with Methylene Blue (MB), nanocarbon, indocyanine green (ICG), and then identified with the aid of an endoscope, an optical instrument, and the like.
and 3, optical identification.
The "advances in rapid identification technology in parathyroid surgery" (wang army rank high-minds et al "chinese tumor clinics" 2019, volume 46, phase 9) documented: the research of Paras et al shows that parathyroid gland has the property of auto-fluorescence in the near infrared region, and when the parathyroid gland is illuminated by light with the wavelength of 785nm, the parathyroid gland can generate the near infrared auto-fluorescence with the wavelength of 820 nm. Ladurner et al report that parathyroid tissue will show near-infrared autofluorescence when parathyroid and surrounding tissues are exposed to 690-770 nm near-infrared light, and by this method, 35 parathyroid glands of 25 patients are distinguished, and 27 parathyroid glands among them are finally accurately identified. In this method, since the penetration depth of near infrared tissue is only a few millimeters (generally within 2 mm), autofluorescence is difficult to observe in parathyroid gland buried deep in tissue during operation.
To the best of the applicant's knowledge, the identification penetration depth of the current parathyroid gland autofluorescence optical identification method is about 2mm, and the identification in the operation needs to be applied by means of the high stripping technology of doctors. Therefore, one of the technical problems to be solved by the present invention is to improve the device identification penetration depth.
Chinese patent application publication No. CN107361744A provides a parathyroid gland identification device and method, wherein the power of the light source laser diode is in milliwatt level (between 80-100mW, 0062, 0080 paragraph).
to the best of the applicant's knowledge, the prior art light sources are all of the milliwatt class, which have the disadvantage that the probe is of the micron order (about 50-500 microns), and emits a recognition spot having a small area (the spot area is comparable to the parathyroid gland size), similar to a point source. Based on: firstly, the identification of parathyroid gland by autofluorescence requires comparing the intensity of several autofluorescence of parathyroid gland, thyroid gland and other tissues (such as muscle and fat), and because the identification light spot in the prior art is too small (even smaller than the area of the identified parathyroid gland), the identification by font fluorescence intensity comparison is often impossible; secondly, the ratio of the area of the identification light spot in the prior art is too small compared with the whole surgical field, so that the parathyroid gland can not be identified by the device in the prior art. Therefore, the second technical problem to be solved by the invention is as follows: on the premise of not burning exposed tissues of a human body, the surface light source is adopted for realizing large-range and large-area identification, the identification accuracy is improved, and the identification time can be shortened.
in the prior art, parathyroid gland is likely to be cut by mistake. The third problem to be solved by the invention is: the spectral imager is provided for rapidly scanning and identifying the cut human tissue, and identifying, positioning and retrieving the planting in time if the parathyroid gland is mistakenly cut.
The fourth problem to be solved by the invention is: the parathyroid gland is judged to be temporarily or permanently hypoparathyroidism by recognizing the blood supply condition of the parathyroid gland, and if the blood supply of the parathyroid gland left in the human body is disconnected, the parathyroid gland is replanted before the operation is closed.
Disclosure of Invention
one of the objects of the present invention is: provides a parathyroid gland identification system, which aims to quickly and accurately identify parathyroid gland by matching a plurality of identification devices and reduce the incidence rate of permanent hypoparathyroidism to the maximum extent.
the second purpose of the invention is that: provides a parathyroid gland recognition device which is applied to each link of the operation and has large recognition range, high speed and accuracy.
the third purpose of the invention is: in the time of planting parathyroid gland, identifying and positioning parathyroid gland which is possibly cut by mistake, planting in time, and reducing the incidence rate of permanent hypoparathyroidism to be within 1 percent.
The invention specifically adopts the technical scheme that:
A parathyroid gland identification device comprises
the autofluorescence excitation device is used for generating 650-810nm exciting light to irradiate human tissues to enable the parathyroid gland to generate autofluorescence; and
the auto-fluorescence acquisition and processing device acquires the auto-fluorescence of the parathyroid gland and processes the auto-fluorescence into identifiable information;
the parathyroid gland identification device is characterized by further comprising a light filtering device, wherein the light filtering device penetrates through the light source with the wavelength of 810nm, so that interference light of the light source cannot penetrate through the light filtering device, and parathyroid gland identification is prevented from being interfered.
According to the prior art and the applicant's knowledge, the fluorescence properties of parathyroid glands: 1) the excitation light wavelength is 650-810nm, and the parathyroid gland can be excited to generate fluorescence; 2) the intensity of the excitation light must reach a threshold value to excite the parathyroid gland to generate fluorescence; 3) the autofluorescence is very weak, and is inevitably affected by the stray light of the excitation light source and cannot be identified.
Applicants have found that even if the laser is not pure light, the intensity of the residual light with wavelength longer than 810nm is in the same order of magnitude as compared with the auto-fluorescence of parathyroid gland, and if the residual light enters the visual field, the parathyroid gland identification is seriously interfered.
the absence of excitation light filtering means in the identification devices of the prior art is an important factor that makes the solutions of the prior art difficult to form into a commercial device that can be used for surgery.
More preferred is: unit of the excitation lightthe optical power of the area is 20-150000mw/cm2。
The optical power of the exciting light per unit area is 20-100mw/cm2The area of a light spot formed by the exciting light is 50-200cm2. This condition is particularly applicable to handheld identification devices.
the optical power of the exciting light per unit area is 100-15000mw/cm2The area of a light spot formed by the exciting light is 0.1-1cm2. This condition is particularly applicable to laser probe-type identification devices.
generally, the higher the optical power of the excitation light, the larger the peak value of autofluorescence generated by the excitation, and the easier the parathyroid gland is to be identified. According to the level of knowledge of those skilled in the art, the intensity of the excitation light should be at a maximum below the maximum threshold that can cause discomfort to the patient or burn exposed tissue, and below the minimum threshold that can excite the parathyroid glands to produce autofluorescence.
The applicant shows through more than 500 clinical trials that the invention selects a small range of optical power per unit area (20-15000 mw/cm)2) After the range is selected, the parathyroid gland can be displayed/identified clearly and sharply, and the probability of clinical misjudgment is reduced to the maximum extent.
wherein, the light source is one or the combination of any several of laser, LED, xenon lamp, halogen lamp, for example: a plurality of lasers can be integrated into a whole to form a light source together;
wherein the light source is one or more lasers, and the power of the single laser is W (watt).
more preferably, the laser is a structured light laser (VCSEL), the single power of the laser can be in watt level, so that the device is more convenient to miniaturize, and another outstanding advantage of the structured light laser is uniform light spots.
A first type of identification device:
The first type of identification means of the present invention is: the parathyroid gland recognition device is used for opening an operation, a wound surface is directly irradiated, light spots generated by a light source basically cover the whole wound surface, and the first recognition device can recognize most parathyroid glands.
The specific scheme is as follows:
A parathyroid gland identification device comprises
the autofluorescence excitation device generates excitation light spots to irradiate human tissues to enable the parathyroid gland to generate autofluorescence;
The auto-fluorescence acquisition and processing device acquires the auto-fluorescence of the parathyroid gland and processes the auto-fluorescence into identifiable information; and
The light filtering device has the transmission wavelength of 810nm, so that interference light of a light source cannot transmit, and parathyroid gland identification is prevented from being interfered;
The device is characterized in that the autofluorescence acquisition and processing device is an infrared camera, the wound surface is imaged, and the bright spot is parathyroid gland; the area of the excitation light spot basically covers the surgical wound surface.
the autofluorescence exciting apparatus comprises a built-in independent light source which is formed by arranging a plurality of lasers.
The infrared camera comprises a lens, and the plurality of lasers and the lens are basically arranged on the same plane.
The plurality of lasers may be arranged in a ring, concentric circle or square arrangement, and it is contemplated that arrangements that enable the individual light sources to emit uniform light intensity are within the scope of the present invention.
The plurality of lasers are arranged together, the heating value is large, and a heat dissipation device can be arranged according to the prior art.
The second scheme of the autofluorescence excitation device is that the autofluorescence excitation device comprises an external light source, the external light source is directly or indirectly connected with a single quartz optical fiber, and the other end of the quartz optical fiber is connected with a lens to convert point-like light into planar light. In this scheme, the external light source may be a laser or a plurality of laser coupled.
or the autofluorescence excitation device comprises an external light source, the external light source comprises a plurality of lasers, each laser is directly or indirectly connected with a plurality of quartz optical fibers, and the other ends of the plurality of optical fibers form a bundle or are connected with a lens.
Or the autofluorescence excitation device comprises an external light source, the external light source is directly or indirectly connected with the plastic optical fiber, and the diameter of the plastic optical fiber is larger than 2 mm.
the scheme is as follows: the first type of identification means is direct irradiation of the wound surface.
Another light source setting mode of the first type of identification device is irradiation from the back of a wound surface, and the specific scheme is as follows:
the autofluorescence excitation device comprises an annular light source for irradiating the back of the wound surface, and the scheme can form a surface light source in the visual field. The other scheme is as follows:
the autofluorescence excitation device comprises a movable light source for irradiating the back of the wound surface.
The second type of recognition means:
The second type of recognition device of the present invention is: when the first device fails to detect four parathyroid glands, the parathyroid glands are further identified by means of close-range irradiation or probes. The excitation light penetration distance of the second type of identification device is several times that of the first type of device. The second type of identification device adopts the specific technical scheme that:
A parathyroid gland identification device comprises
The autofluorescence excitation device generates excitation light spots to irradiate human tissues to enable the parathyroid gland to generate autofluorescence;
The auto-fluorescence acquisition and processing device acquires the auto-fluorescence of the parathyroid gland and processes the auto-fluorescence into identifiable information; and
the light filtering device has the transmission wavelength of 810nm, so that interference light of a light source cannot transmit, and parathyroid gland identification is prevented from being interfered;
The autofluorescence excitation device is characterized by comprising a light source;
The device also comprises a laser probe, wherein the laser probe is provided with optical fibers, one optical fiber is connected with a light source and is called as a luminous optical fiber, and the optical power of the exciting light per unit area is 100-15000mw/cm2The light spot formed by the exciting lightThe area is 0.1-1cm2。
in the operation, after the wound surface is cut, the maximum embedding depth of the parathyroid gland is 6mm, and the scheme of the invention is verified to be characterized in that the detection depth can reach 6mm, which is enough to meet the operation requirement, and meanwhile, the detection depth is several times of that of the prior art.
In the second device, the autofluorescence collecting and processing device comprises an external camera, irradiates the wound surface, collects the parathyroid autofluorescence, and processes into recognizable image information.
More preferably, the autofluorescence collecting and processing device further comprises a spectrum analysis device, the laser probe is provided with two optical fibers, one of the optical fibers is connected with the light source and is called as a luminescent optical fiber, and the other optical fiber is connected with the spectrum analysis device and is called as a lighting optical fiber.
Wherein the spectrum analysis device is one of a spectrometer, a photomultiplier tube or an APD photodetector; the spectral analysis device returns autofluorescence through the collection lighting optical fiber, and determines the position of the parathyroid gland by analyzing the wavelength and intensity value of the special spectrum of the parathyroid gland.
The autofluorescence collecting and processing device also comprises an alarm device, and when the spectral analysis device determines the position of the parathyroid gland, the alarm device sends out alarm information to prompt a doctor.
In another aspect, the laser probe includes a handheld portion, and a camera connected to an image display device, for example: image display devices such as computers and mobile phones. The scheme is to miniaturize an external camera and arrange the external camera on a laser probe.
According to a more preferable scheme, the autofluorescence acquisition and processing device comprises a laser generating mechanism, a transmission mechanism and a laser probe, wherein the laser generating mechanism comprises a plurality of lasers, a power supply module for supplying power to the lasers, a control module for controlling the power supply module and a first wireless module; the transmission mechanism comprises an optical fiber, one end of the optical fiber is connected with the laser, and the other end of the optical fiber is connected with the laser probe; the laser probe comprises a collimator, a control panel and a second communication module, the collimator is connected with the transmission mechanism, the second communication module is wirelessly connected with the first communication module, and the control panel is wirelessly connected with the control module through the second communication module;
wherein, an optical filter is also arranged between the laser and the optical fiber;
wherein the wavelength emitted by the laser is 785nm, and the power is 1.5W;
wherein the number of the lasers is 4;
wherein the optical fiber is a pmma plastic optical fiber.
Another scheme of the second type of identification device is a handheld detection device, which adopts the specific scheme that:
A parathyroid gland identification device comprises
The autofluorescence excitation device generates excitation light spots to irradiate human tissues to enable the parathyroid gland to generate autofluorescence;
The auto-fluorescence acquisition and processing device acquires the auto-fluorescence of the parathyroid gland and processes the auto-fluorescence into identifiable information; and
the light filtering device has the transmission wavelength of 810nm, so that interference light of a light source cannot transmit, and parathyroid gland identification is prevented from being interfered;
Characterized in that the luminous power per unit area of the exciting light is 100-15000mw/cm2the area of a light spot formed by the exciting light is 0.1-1cm2。
the autofluorescence excitation device, the autofluorescence acquisition processing device and the filtering device are integrated in the shell, wherein the autofluorescence acquisition processing device comprises a camera, the camera comprises a lens, and the lens is integrated with the autofluorescence excitation device.
Wherein the autofluorescence excitation device comprises a built-in light source, the built-in light source comprises a plurality of lasers, and the plurality of lasers are integrated with a lens.
The hand-held detection device can be set as an external light source, and the scheme is as follows: the autofluorescence excitation device comprises an external light source, the external light source is directly or indirectly connected with a single quartz optical fiber, and the other end of the quartz optical fiber is connected with a lens to convert point-like light into planar light. In this scheme, the external light source may be a laser or a plurality of laser coupled.
And the second scheme is that the autofluorescence excitation device comprises an external light source, the external light source comprises a plurality of lasers, each laser is directly or indirectly connected with a plurality of quartz optical fibers, and the other ends of the plurality of optical fibers form a bundle or are connected with a lens.
and according to the third scheme, the autofluorescence excitation device comprises an external light source, wherein the external light source is directly or indirectly connected with a plastic optical fiber, and the diameter of the plastic optical fiber is larger than 2 mm.
More preferred is:
A parathyroid gland recognition device comprises a shell, wherein the front end of the shell is a detection end, the rear end of the shell is a gripping end, the detection end is provided with an infrared fluorescence excitation mechanism used for exciting autofluorescence of a human body, a light source collection mechanism and a circuit board used for collecting the autofluorescence of the human body and generating images are arranged in the shell, the light source collection mechanism comprises a camera arranged in the shell and a lens arranged at the detection end and connected with the camera, the circuit board is connected with the infrared fluorescence excitation mechanism and the light source collection mechanism through circuits, the circuit board comprises a control module, a communication module and a power supply module, and the modules are connected through circuits, wherein the control module is used for controlling the infrared fluorescence excitation mechanism, the light source collection mechanism and the modules;
The communication module is connected with the computer through a wireless signal and used for transmitting the image generated by the light source collecting mechanism to the computer and controlling the light source collecting mechanism through the computer;
the power supply module is used for supplying power to the infrared fluorescence excitation mechanism, the light source collection mechanism and each module; preferably, the infrared fluorescence excitation mechanism comprises a lamp panel arranged at the front end of the shell, a lens through hole is formed in the middle of the lamp panel, a focusing lens is arranged at the front end of the lamp panel in parallel, and a plurality of infrared emission tubes are arranged on the surface of the lamp panel between the focusing lens and the lamp panel around the lens through hole;
The lens is arranged in the lens through hole;
preferably, the camera comprises a photosensitive element, a light filtering mechanism is arranged in front of the photosensitive element, the light filtering mechanism comprises a gear turntable, a motor gear and a motor, a rotating shaft is arranged at the center of the gear turntable, the gear turntable and the motor gear are meshed with each other, the axis of the motor gear is connected with an output shaft of the motor, the motor drives the motor gear to rotate, so that the meshed gear turntable is driven to rotate along the rotating shaft, a plurality of light filter through holes are formed in the gear turntable around the rotating shaft, when the turntable rotates to a corresponding position, the light filter through holes are aligned with the photosensitive element, and light filters are arranged in the light;
Preferably, the lamp panel is made of aluminum;
Preferably, the infrared emission tubes are arranged in an annular array, and the number of the infrared emission tubes is 20-100;
preferably, the control module is a single chip microcomputer;
preferably, the number of the optical filter through holes is two, and an optical filter A with a light-transmitting waveband of 810-1000nm and an optical filter B with a light-transmitting waveband of 700-1000nm are respectively arranged in the two optical filter through holes;
Preferably, the cut-off depths of the filter A and the filter B are both OD4-OD 6;
preferably, the shell is provided with an adjusting switch, the adjusting switch is connected with the circuit board through a circuit, the gripping end is provided with a human body mechanical handle, a battery bin is arranged inside the handle, and the battery bin is connected with the power module through a circuit.
The third type of recognition means:
The third type of recognition device of the invention has the application scenarios that: after thyroidectomy, the parathyroid glands were examined for damage. Firstly, a fluorescent drug (methylene blue or ICG) is injected intravenously, and then fluorescence emitted by the methylene blue or ICG is detected by a laser probe or a handheld recognition device to judge whether the drug enters the parathyroid gland, namely whether the blood circulation of the parathyroid gland is damaged in the operation.
The structure of this type of identification device is substantially the same as that of a hand-held identification device, and a third type of identification device is used by first injecting a fluorescent drug intravenously, for example: MB-methylene blue (690 nm central wavelength), ICG-indole (830 nm central wavelength).
The clinical significance of the third category of identification devices is: for parathyroid gland with good blood circulation, the parathyroid gland can survive, and doctors can close the wound with confidence; for parathyroid glands that are not blood-moving well, transplantation can be considered, where the species is in a well-moving position, to allow survival.
fourth type recognition means:
although the above scheme is adopted, the parathyroid gland is inevitably cut by mistake in the operation, the fourth type of identification device is adopted to scan and explore the cut tissue, and the parathyroid gland can be found back and planted in the planting period.
at present, the operation is more and more from open to endoscopic operation, at this time, the parathyroid gland is easy to be cut by mistake, and the parathyroid gland can be found back and planted by utilizing the technology.
the fourth type of identification device is called as a surface imaging fluorescence spectrum analyzer or a matrix fluorescence spectrum analyzer in trial use, is different from the traditional point type fluorescence spectrum analyzer, changes the traditional one-dimensional analysis into two-dimensional analysis, and obviously improves the identification speed of the specimen so as to adapt to the transient time window (usually within 15 minutes) for planting the parathyroid gland. The fourth type of identification device adopts the specific technical scheme that:
a parathyroid gland identification device comprises
The autofluorescence excitation device generates excitation light spots to irradiate human tissues to enable the parathyroid gland to generate autofluorescence;
the auto-fluorescence acquisition and processing device acquires the auto-fluorescence of the parathyroid gland and processes the auto-fluorescence into identifiable information; and
the filter device can prevent the interference light of the light source from transmitting, and avoid the interference of parathyroid gland identification;
The autofluorescence excitation device is characterized by comprising a light source, wherein the light source is an LED light source, a semiconductor point laser, a laser or a vcsel uniform field intensity laser;
The filtering device is a narrow-band filter with the wavelength of 785nm and is used for filtering light source residual light or interference light;
The optical power of the exciting light per unit area is 20-150000mw/cm2。
The autofluorescence acquisition and processing device comprises a photoelectric conversion device, wherein the photoelectric conversion device is a spectrometer, a photomultiplier or an APD (avalanche photo diode) photodetector.
Wherein the photoelectric conversion devices are provided in one; when the scheme is used, a photoelectric conversion device surface scanning mode is adopted, and parathyroid glands which are possibly cut by mistake are identified for a long time;
Alternatively, the photoelectric conversion devices are arranged in a row; the scanning speed of the scheme is high;
Alternatively, the photoelectric conversion devices are arranged in rows and columns to form a panel, which has shorter scanning time and higher cost.
Another embodiment of the photoelectric conversion device is:
The autofluorescence collecting and processing device comprises a plurality of groups of same cameras, wherein optical filters with different wavelengths are arranged in front of each camera lens, and the wavelengths of the optical filters are 900 +/-10 nm, 830 +/-10 nm and 670 +/-10 nm.
in a fourth type of identification device, the device further comprises a tray, wherein the supporting tube is transparent on both sides, a specimen is clamped in the supporting tube, and the thickness of the specimen is within 4 mm. The arrangement is such that the thickness of the tissue covered by the parathyroid gland does not exceed 1mm, and the detection means of the present invention is fully capable of detecting. In this scheme, both the photoelectric conversion device and the tray can be moved to complete scanning.
the fourth type of identification device further comprises a dark box. All the components of the fourth type of identification means are arranged in a dark box.
one preferred way is:
a parathyroid identification device comprising a dark box, the dark box including therein:
The light source mechanism is used for providing a light source for exciting fluorescence of a sample and comprises a plurality of light sources with different wavelengths;
an imaging mechanism for collecting an image of the autofluorescence excited by the sample, the infrared camera being capable of switching a wavelength band of the received light;
The sample tray is used for placing a sample;
The light source mechanism is matched with the imaging mechanism, the device further comprises a computer, a display screen and a power supply, the computer is arranged outside the sample bin and is respectively connected with the light source mechanism and the imaging mechanism, the display screen is connected with the computer, and the power supply is respectively connected with the light source mechanism, the imaging mechanism, the computer and the display screen;
The LED light source mechanism comprises a light source turntable, light source through holes and a plurality of LEDs, wherein the LEDs are uniformly distributed on a concentric circle of the light source turntable, the light source through holes are aligned with one LED, the LEDs aligned with the light source through holes can be switched by rotating the light source turntable, and the diameter of each light source through hole is matched with the diameter of the LED; the light source turntable is controlled by the computer;
Wherein the number of the LEDs is 9;
Wherein the emission wavelengths of the LEDs are respectively 660nm, 680nm, 700nm, 720nm, 740nm, 760nm, 780nm, 800nm and 808 nm;
The imaging mechanism comprises an imaging turntable, imaging through holes and a plurality of fluorescent cameras, the fluorescent cameras are uniformly distributed on a concentric circle of the imaging turntable, the imaging through holes are aligned with one fluorescent camera, the fluorescent cameras aligned with the imaging through holes can be switched by rotating the imaging turntable, and the diameter of each imaging through hole is matched with the size of a lens of each fluorescent camera;
The imaging turntable is controlled by the computer;
wherein the number of the fluorescence cameras is 8;
wherein the light receiving wave bands of the fluorescence camera are 685-715nm, 705-735nm, 725-755nm, 745-775nm, 765-795nm, 785-815nm, 805-835nm and 825-855nm respectively;
The light source mechanism and the imaging mechanism are arranged at the top of the detection camera bellows, the sample tray is arranged at the bottom of the detection camera bellows, and the position of the sample tray corresponds to the position of the imaging mechanism;
The sample tray is made of a light absorption material, and the light absorption material can absorb light with a wave band of 660-808 nm;
And the fluorescence image collected by the imaging mechanism is transmitted to a computer, and is transmitted to a display screen for display after being processed by the computer.
The invention can solve the four technical problems pointed out in the prior art, and has the following beneficial effects:
The method has good identification effect, so that for the patient with secondary hyperthyroidism, the parathyroid gland needs to be resected in the process, and the method can identify and resect the secondary hyperthyroidism which is difficult to find by a doctor.
Drawings
FIG. 1 is a spectral analysis of parathyroid and peripheral tissues;
FIG. 2 is a spectral analysis (histogram) of parathyroid and peripheral tissues;
FIG. 3 is a spectral diagram of a laser light source;
FIG. 4 is a block diagram of a first type of identification device;
FIG. 5 is a schematic diagram of a backlight source;
FIG. 6 is a schematic diagram of a movable backlight source;
FIG. 7 is a comparison of the present invention with a prior art light source;
FIG. 8 is a schematic structural view of a laser probe;
FIG. 9 is a schematic diagram of a large diameter plastic fiber laser probe;
FIG. 10 is a schematic view of a hand held light source configuration (multiple optical fibers);
FIG. 11 is a schematic view of a hand held light source (one fiber);
FIG. 12 is a schematic diagram of the structure of the spectrometer (single-sided photoreceptor);
FIG. 13 is a schematic diagram of the spectrometer configuration (double-sided photoreceptor);
FIG. 14 is a schematic structural view of a tray;
FIG. 15 is a schematic view of the structure of the electro-optical converting apparatus (camera scheme);
FIG. 16-1, FIG. 16-2, FIG. 16-3 are schematic diagrams of the optoelectronic conversion device (spectrometer, PDA and photomultiplier solution);
FIG. 17 is a schematic view of the structure of the electro-optical changing device (galvanometer mechanism);
FIG. 18 is a photograph of a product of the present invention during a clinical trial, intraoperative real-time exploration;
FIG. 19 is a photograph of a parathyroid gland of the present invention showing the presence of a miscut in excised tissue during clinical trials.
Detailed Description
after the sample is finished, the Shandong university is entrusted to carry out spectrum detection on the parathyroid related organ, and the name of the detection equipment is as follows: the solar simulator F-4600 is a commercial test unit of the applicant, Jinan micro intelligent science and technology, the test unit tests spectral data of parathyroid organs of 7 batches to obtain parathyroid research data, and the applicant manufactures figures 1 and 2 (schematic diagrams) according to the parathyroid research data.
as shown in fig. 1 and 2, 1 is a parathyroid gland autofluorescence characteristic spectrum, 2 is a thyroid gland autofluorescence characteristic spectrum, 3 is a muscle autofluorescence characteristic spectrum, 4 is a fat autofluorescence characteristic spectrum, and 5 is a trachea autofluorescence characteristic spectrum.
As can be seen from FIGS. 1 and 2, under the conditions of the present invention, the peak intensity of the auto-fluorescence of parathyroid gland is highest under 822nm, and other tissues are lower, i.e. only after the auto-fluorescence of various human tissues is detected, a bright spot of parathyroid gland can be formed on the image or the position of parathyroid gland can be confirmed by spectral analysis and comparison.
in addition, through the research of the inventor, the light power range per unit area of the exciting light is 20-15000mw/cm2The parathyroid gland can be displayed/identified clearly and sharply, and the probability of clinical misjudgment is reduced to the maximum extent.
FIG. 3 shows a spectrum 6 of a laser light source having a wavelength of 785nm, and it can be seen from FIG. 3 that even in the case of the laser light source, there is still residual light or interfering light having a wavelength of more than 785nm, and if this interfering light is not excluded, the parathyroid gland cannot be identified.
FIG. 4 is a block diagram of the apparatus of the present invention, wherein the autofluorescence excitation device generates excitation light, the excitation light is filtered by the filter device to remove interfering light, then a light spot is formed to irradiate human tissue, so that the parathyroid gland generates autofluorescence, and the autofluorescence acquisition and processing device acquires the autofluorescence of the parathyroid gland and processes the autofluorescence into identifiable information.
Fig. 5 is a schematic structural view of a backlight light source for the first type of identification device, a plurality of light sources 7 are integrated on a ring-shaped panel 8, and when the device is applied, the device is placed on the back of an operation opening to form a surface light source on an operation field.
fig. 6 is a schematic structural view of a movable backlight light source, wherein the light source 7 is arranged on a movable body 9, scans the back of the wound surface and forms a surface light source in the surgical field.
Fig. 7 is a comparison diagram of the present invention and a background art light source, specifically, a comparison between the technical scheme of the chinese patent application publication No. CN107361744A and the present invention, where 11 is a surgical wound, 10 is an identification spot of the background technical scheme, which is similar to a point light source, and 12 is an identification spot of the present invention, which is a surface light source.
FIG. 8 is a schematic structural diagram of a laser probe, in which an external light source 17 is connected to a light-emitting fiber 19-1, the other end of the light-emitting fiber 19-1 is connected to a lens 20 for illuminating a wound surface, and the laser probe further includes a light-collecting fiber 19-2, one end of the light-collecting fiber 19-2 is connected to the lens 20, and the other end is connected to a spectrum analysis device for assisting in identifying parathyroid gland.
The scheme also comprises a handheld part, the handheld part comprises a shell 14, a light inlet optical fiber 19-1 and a lighting optical fiber 19-2 are fixed on the shell, the shell is generally detachably connected and convenient for replacing the optical fibers, a camera 16 is further arranged on the shell 14, an image formed by the camera 16 is transmitted to a monitor 13, parathyroid gland is identified according to bright light spots, and a control panel 15 is further arranged on the shell 14.
fig. 9 is a schematic structural diagram of a large-diameter plastic optical fiber laser probe, which is basically the same as the embodiment of fig. 8, except that the light inlet optical fiber 19-1 is a large-diameter plastic optical fiber, and in this case, the optical fiber can form a surface light source, and the lens 20 is omitted.
fig. 10 is a schematic structural diagram of a handheld light source, which includes an external light source 21, a plurality of lasers integrated on the light source 21, each laser connected with an optical fiber, and a lens 22 connected to the optical fiber, wherein the plurality of lenses 22 and a camera lens 24 are integrated on a panel 23.
Fig. 11 is a schematic structural diagram of a handheld light source, and a plurality of lasers are integrated on an external light source 21, the lasers are coupled together and connected with a lens 22 through an optical fiber, and the lens 22 and a camera lens 24 are integrated on a panel 23.
fig. 12 is a schematic structural diagram of a spectrometer, which includes a dark box 25, a photoelectric conversion device 27 (or a group of rows or a panel type of transverse rows and longitudinal columns) is disposed on the dark box 25, a tray 26 is disposed in the dark box 25, and excised human tissues to be identified are placed in the tray 26.
The scheme of fig. 13 is basically the same as that of fig. 12, except that two photoelectric conversion devices 27 are arranged on the upper and lower surfaces of the dark box 25, and the up-and-down recognition is performed simultaneously, so that the recognition speed is higher.
fig. 14 is a schematic structural view of the tray 28, which includes upper and lower transparent panels that sandwich excised tissue to be identified.
Fig. 15 shows a photoelectric conversion device using a camera scheme, a plurality of cameras 29 are arranged together with a light source 30, optical filters with different wavelengths are arranged in front of the lens of each camera, each camera 29 can only image the autofluorescence with specific wavelength, and the parathyroid gland positions can be identified by superposing a plurality of images together.
Fig. 16-1, 16-2, 16-3 are schematic structural diagrams of photoelectric conversion devices, wherein the photoelectric conversion devices can be one of a spectrometer, a PDA and a photomultiplier, fig. 16-1 is a scheme of being arranged in a row, fig. 16-2 is a scheme of being transversely arranged in a row and being longitudinally arranged in a row, and fig. 16-3 is a scheme of one photoelectric conversion device.
fig. 17 is a schematic structural diagram of the photoelectric conversion device of the galvanometer mechanism, which includes an external light source 32 for irradiating excised tissues, and a galvanometer 33 arranged below the photoelectric conversion device 31.
Fig. 18 is a real-time examination chart in clinical operation, and two bright spots are identified at an operation opening by using the sample handheld identification device, namely the parathyroid gland.
FIG. 19 shows the finding of a miscut parathyroid gland in excised tissue.
Claims (50)
1. A parathyroid gland identification device comprises
The autofluorescence excitation device is used for generating 650-810nm exciting light to irradiate human tissues to enable the parathyroid gland to generate autofluorescence; and
The auto-fluorescence acquisition and processing device acquires the auto-fluorescence of the parathyroid gland and processes the auto-fluorescence into identifiable information;
the parathyroid gland identification device is characterized by further comprising a light filtering device, wherein the light filtering device penetrates through the light source with the wavelength of 810nm, so that interference light of the light source cannot penetrate through the light filtering device, and parathyroid gland identification is prevented from being interfered.
2. The parathyroid recognition device of claim 1, wherein the optical power per unit area of the excitation light is from 20 to 150000mw/cm2。
3. the parathyroid recognition device of claim 1, wherein the excitation light has an optical power per unit area of 20-100mw/cm2the area of a light spot formed by the exciting light is 50-200cm2。
4. The parathyroid recognition device of claim 1, wherein the optical power per unit area of the excitation light is from 100-15000mw/cm2The area of a light spot formed by the exciting light is 0.1-1cm2。
5. the parathyroid gland identification device of claim 1, wherein the autofluorescence excitation device includes a light source, and the light source is one or a combination of any of a laser, an LED, a xenon lamp and a halogen lamp.
6. The parathyroid recognition device of claim 5, wherein the light source is one or more lasers, the power of the single laser being of the W class.
7. The parathyroid recognizing device of claim 5 or 6, wherein the laser is a structured light laser (VCSEL).
8. A parathyroid gland identification device comprises
The autofluorescence excitation device generates excitation light spots to irradiate human tissues to enable the parathyroid gland to generate autofluorescence;
the auto-fluorescence acquisition and processing device acquires the auto-fluorescence of the parathyroid gland and processes the auto-fluorescence into identifiable information; and
The light filtering device has the transmission wavelength of 810nm, so that interference light of a light source cannot transmit, and parathyroid gland identification is prevented from being interfered;
The device is characterized in that the autofluorescence acquisition and processing device is an infrared camera, the wound surface is imaged, and the bright spot is parathyroid gland; the area of the excitation light spot basically covers the surgical wound surface.
9. The parathyroid recognition device of claim 8, wherein the autofluorescence excitation means includes a built-in self-contained light source comprising a plurality of lasers arranged in an array.
10. the parathyroid recognition device of claim 9, wherein the infrared camera includes a lens, the plurality of lasers being substantially co-planar with the lens.
11. the parathyroid recognition device of claim 10, wherein the plurality of lasers are in a circular, concentric circular or square arrangement.
12. a parathyroid recognizing device according to any one of claims 9 to 11, characterised in that a heat sink is provided to provide heat sinking of the plurality of lasers.
13. the parathyroid gland identification device of claim 8, wherein the autofluorescence excitation device includes an external light source, the external light source is directly or indirectly connected to a single quartz fiber, and the other end of the quartz fiber is connected to a lens to convert point-like light into planar light.
14. the parathyroid recognition device of claim 13, wherein the external light source is a laser or a plurality of laser couplings.
15. The parathyroid gland identification device of claim 8, wherein the autofluorescence excitation device includes an external light source, the external light source includes a plurality of lasers, each laser is directly or indirectly connected to a quartz fiber, and the other ends of the plurality of fibers are bundled or connected to a lens.
16. The parathyroid recognition device of claim 8, wherein the autofluorescence excitation device includes an external light source, the external light source being directly or indirectly connected to a plastic optical fiber, the plastic optical fiber having a diameter greater than 2 mm.
17. the parathyroid recognition device of claim 8, wherein the autofluorescence excitation device includes an annular light source for illuminating the back of the wound.
18. The parathyroid recognition device of claim 8, wherein the autofluorescence excitation device includes a movable light source for illuminating the back of the wound.
19. A parathyroid gland identification device comprises
the autofluorescence excitation device generates excitation light spots to irradiate human tissues to enable the parathyroid gland to generate autofluorescence;
The auto-fluorescence acquisition and processing device acquires the auto-fluorescence of the parathyroid gland and processes the auto-fluorescence into identifiable information; and
the light filtering device has the transmission wavelength of 810nm, so that interference light of a light source cannot transmit, and parathyroid gland identification is prevented from being interfered;
The autofluorescence excitation device is characterized by comprising a light source;
the device also comprises a laser probe, wherein the laser probe is provided with optical fibers, one optical fiber is connected with a light source and is called as a luminous optical fiber, and the optical power of the exciting light per unit area is 100-15000mw/cm2The area of a light spot formed by the exciting light is 0.1-1cm2。
20. The parathyroid gland identification device of claim 19, wherein the autofluorescence acquisition and processing means includes an external camera for illuminating the wound surface, acquiring parathyroid gland autofluorescence, and processing into identifiable image information.
21. the parathyroid gland identification device of claim 19, wherein the autofluorescence collecting and processing device further comprises a spectral analysis device, the laser probe has two optical fibers, one of the optical fibers is connected with the light source and is called a light emitting optical fiber, and the other optical fiber is connected with the spectral analysis device and is called a light collecting optical fiber.
22. The parathyroid recognition device of claim 21, wherein the spectral analysis device is one of a spectrometer, photomultiplier tube or APD photodetector; the spectral analysis device returns autofluorescence through the collection lighting optical fiber, and determines the position of the parathyroid gland by analyzing the wavelength and intensity value of the special spectrum of the parathyroid gland.
23. The parathyroid gland identification device of claim 19, wherein the autofluorescence collecting and processing device further comprises an alarm device, and when the spectral analysis device determines the parathyroid gland position, an alarm message is sent to prompt a doctor.
24. The parathyroid recognition device of claim 19, wherein the laser probe includes a hand-held portion, and further including a miniature camera on the hand-held portion, the miniature camera being connected to an image display device.
25. a parathyroid gland identification device comprises
the autofluorescence excitation device generates excitation light spots to irradiate human tissues to enable the parathyroid gland to generate autofluorescence;
The auto-fluorescence acquisition and processing device acquires the auto-fluorescence of the parathyroid gland and processes the auto-fluorescence into identifiable information; and
the light filtering device has the transmission wavelength of 810nm, so that interference light of a light source cannot transmit, and parathyroid gland identification is prevented from being interfered;
Characterized in that the luminous power per unit area of the exciting light is 100-15000mw/cm2The area of a light spot formed by the exciting light is 0.1-1cm2。
26. The parathyroid identification device of claim 25, wherein the autofluorescence excitation means, autofluorescence acquisition and processing means and filtering means are integrated within a housing.
27. the parathyroid recognition device of claim 26, wherein the autofluorescence acquisition processing device includes a camera including a lens, the lens being integrated with the autofluorescence excitation device.
28. The parathyroid recognition device of claim 27, wherein the autofluorescence excitation device includes a built-in light source including a plurality of lasers integrated with a lens.
29. The parathyroid gland identification device of claim 25, wherein the autofluorescence inducing device includes an external light source, the external light source is directly or indirectly connected to a single quartz fiber, and the other end of the quartz fiber is connected to a lens to convert point-like light into planar light. In this scheme, the external light source may be a laser or a plurality of laser coupled.
30. the parathyroid gland identification device of claim 25, wherein the autofluorescence excitation device includes an external light source, the external light source includes a plurality of lasers, each laser is directly or indirectly connected to a plurality of quartz optical fibers, and the other ends of the plurality of optical fibers are bundled or connected to a lens.
31. The parathyroid recognition device of claim 25, wherein the autofluorescence excitation device includes an external light source, the external light source being directly or indirectly connected to a plastic optical fiber, the plastic optical fiber having a diameter greater than 2 mm.
32. a parathyroid gland recognition device is characterized by comprising a shell, wherein the front end of the shell is a detection end, the rear end of the shell is a gripping end, the detection end is provided with an infrared fluorescence excitation mechanism for exciting the autofluorescence of a human body, a light source collection mechanism and a circuit board for collecting the autofluorescence of the human body and generating images are arranged in the shell, the light source collection mechanism comprises a camera arranged in the shell and a lens arranged at the detection end and connected with the camera, the circuit board is connected with the infrared fluorescence excitation mechanism and the light source collection mechanism through circuits, the circuit board comprises a control module, a communication module and a power supply module, and the control module is used for controlling the infrared fluorescence excitation mechanism, the light source collection mechanism and the modules; the communication module is connected with the computer through a wireless signal and used for transmitting the image generated by the light source collecting mechanism to the computer and controlling the light source collecting mechanism through the computer.
33. The parathyroid recognition device of claim 32, wherein the power module is adapted to power the infrared fluorescence excitation mechanism, the light source collection mechanism and each module.
34. The parathyroid gland identification device of claim 32, wherein the infrared fluorescence excitation mechanism comprises a lamp panel disposed at the front end of the housing, a lens through hole is disposed at the middle of the lamp panel, a focusing lens is disposed in parallel at the front end of the lamp panel, and a plurality of infrared emission tubes are disposed around the lens through hole on the surface of the lamp panel between the focusing lens and the lamp panel.
35. The parathyroid recognition device of claim 32, wherein the lens is disposed within the lens aperture.
36. the parathyroid gland identification device of claim 32, wherein the camera includes a light sensing element, a light filtering mechanism is disposed in front of the light sensing element, the light filtering mechanism includes a gear wheel, a motor gear and a motor, the gear wheel has a central axis of rotation, the gear wheel is engaged with the motor gear, the motor gear is connected to an output shaft of the motor, the motor drives the motor gear to rotate, so as to drive the engaged gear wheel to rotate along the axis of rotation, the gear wheel has a plurality of filter through holes around the axis of rotation, when the wheel rotates to a corresponding position, the filter through holes are aligned with the light sensing element, and the filter through holes are disposed with filters.
37. The parathyroid recognition device of claim 32, wherein the infrared emitting tubes are in an annular array of 20-100 tubes.
38. the parathyroid recognition device of claim 32, wherein there are two of the filter through holes, and wherein there are filter a with a transmission wavelength band of 810-1000nm and filter B with a transmission wavelength band of 700-1000 nm.
39. The parathyroid gland identification device of claim 32, wherein the housing has a regulating switch connected to the circuit board via a circuit, the grasping end has a biomechanical grip, and the grip has a battery compartment therein connected to the power module via a circuit.
40. a parathyroid gland identification device comprises
The autofluorescence excitation device generates excitation light spots to irradiate human tissues to enable the parathyroid gland to generate autofluorescence;
The auto-fluorescence acquisition and processing device acquires the auto-fluorescence of the parathyroid gland and processes the auto-fluorescence into identifiable information; and
The filter device can prevent the interference light of the light source from transmitting, and avoid the interference of parathyroid gland identification;
the autofluorescence excitation device is characterized by comprising a light source, wherein the light source is an LED light source, a semiconductor point laser, a laser or a vcsel uniform field intensity laser;
The filtering device is a narrow-band filter with the wavelength of 785 nm;
the optical power of the exciting light per unit area is 20-150000mw/cm2。
41. The parathyroid gland identification device of claim 40, wherein the autofluorescence collection and processing device includes a photoelectric conversion device, the photoelectric conversion device is a spectrometer, a photomultiplier tube or an APD photodetector.
42. The parathyroid gland identification device of claim 40, wherein the autofluorescence collection processing device includes multiple sets of identical cameras, each camera having a different wavelength filter in front of the lens, the filters having wavelengths of 900 ± 10nm, 830 ± 10nm and 670 ± 10 nm.
43. The parathyroid recognition device of claim 40, further including a tray, the stem being transparent on both sides and having a specimen sandwiched therebetween, the specimen having a thickness within 4 mm.
44. The parathyroid identification device of claim 40, further including a dark box.
45. a parathyroid identification device comprising a dark box, the dark box including therein:
the light source mechanism is used for providing a light source for exciting fluorescence of a sample and comprises a plurality of light sources with different wavelengths;
An imaging mechanism for collecting an image of the autofluorescence excited by the sample, the imaging mechanism being capable of switching a wavelength band of the received light;
The sample tray is used for placing a sample;
The light source mechanism is matched with the imaging mechanism, and the device further comprises a computer, a display screen and a power supply.
46. The parathyroid gland identification device of claim 45, wherein the computer is disposed outside the sample compartment, the computer is connected to the light source mechanism and the imaging mechanism, respectively, the display screen is connected to the computer, and the power source is connected to the light source mechanism, the imaging mechanism, the computer and the display screen, respectively;
the LED light source mechanism comprises a light source turntable, light source through holes and a plurality of LEDs, wherein the LEDs are uniformly distributed on a concentric circle of the light source turntable, the light source through holes are aligned with one LED, and the diameter of the LED light source through holes aligned with the light source through holes can be switched to be matched with the diameter of the LEDs by rotating the light source turntable;
the light source turntable is controlled by the computer; wherein the number of the LEDs is 9;
Wherein the emission wavelengths of the LEDs are respectively 660nm, 680nm, 700nm, 720nm, 740nm, 760nm, 780nm, 800nm and 808 nm;
the imaging mechanism comprises an imaging turntable, imaging through holes and a plurality of fluorescent cameras, the fluorescent cameras are uniformly distributed on a concentric circle of the imaging turntable, the imaging through holes are aligned with one fluorescent camera, the fluorescent cameras aligned with the imaging through holes can be switched by rotating the imaging turntable, and the diameter of each imaging through hole is matched with the size of a lens of each fluorescent camera; the imaging turret is controlled by the computer.
47. The parathyroid recognition device of claim 46, wherein the number of fluorescence cameras is 8; the light receiving wave bands of the fluorescence camera are 685-715nm, 705-735nm, 725-755nm, 745-775nm, 765-795nm, 785-815nm, 805-835nm and 825-855nm respectively.
48. the parathyroid recognition device of claim 47, wherein the light source means and imaging means are located at the top of the testing camera, the sample tray is located at the bottom of the testing camera, and the position of the sample tray corresponds to the position of the imaging means.
49. A parathyroid gland identification system comprising a parathyroid gland identification means according to claim 8, claim 19 or claim 40.
50. A parathyroid gland identification system comprising a parathyroid gland identification means according to claim 8, claim 25 or claim 40.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/CN2019/119012 WO2020199605A1 (en) | 2019-04-04 | 2019-11-16 | Parathyroid gland recognition device and system |
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2019102694170 | 2019-04-04 | ||
| CN201910269417.0A CN109864715A (en) | 2019-04-04 | 2019-04-04 | A kind of hand-held human body spontaneous fluorescence detection device |
| CN201910582533 | 2019-06-29 | ||
| CN2019105825338 | 2019-06-29 | ||
| CN201921039946 | 2019-07-04 | ||
| CN2019210399463 | 2019-07-04 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110558938A true CN110558938A (en) | 2019-12-13 |
Family
ID=68774219
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910771976.1A Pending CN110558938A (en) | 2019-04-04 | 2019-08-21 | Parathyroid gland recognition device and system |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN110558938A (en) |
| WO (1) | WO2020199605A1 (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112155562A (en) * | 2020-10-20 | 2021-01-01 | 湖南马尼克医疗科技有限公司 | Parathyroid gland composite detection device |
| CN113067240A (en) * | 2021-03-24 | 2021-07-02 | 山东大学 | High repetition frequency self-mode-locking aureoemer laser and application thereof in parathyroid gland identification |
| CN113662515A (en) * | 2021-09-22 | 2021-11-19 | 赛德生物科技(山东)有限责任公司 | Parathyroid gland check out test set |
| CN116138744A (en) * | 2023-04-21 | 2023-05-23 | 北京航空航天大学 | Autofluorescence detection probe and system for detecting parathyroid tissue in vivo |
| WO2023222268A1 (en) * | 2022-05-19 | 2023-11-23 | Fluoptics | Medical imaging device and method suitable for observing a plurality of spectral bands |
| EP4110167A4 (en) * | 2020-02-25 | 2024-01-24 | Vanderbilt University | Methods of using optical fiber-based fluorescence spectroscopy for surgical guidance and/or tissue diagnostics and applications of same |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203634145U (en) * | 2013-12-31 | 2014-06-11 | 中国人民武装警察部队总医院 | Fluorescence endoscopy imaging system |
| CN107361744A (en) * | 2017-07-31 | 2017-11-21 | 清华大学 | The identification device and method of a kind of parathyroid gland |
| WO2018126114A2 (en) * | 2016-12-29 | 2018-07-05 | Vanderbilt University | Methods and apparatus for intraoperative assessment of parathyroid gland vascularity using laser speckle contrast imaging and applications of same |
| CN108523849A (en) * | 2018-05-02 | 2018-09-14 | 南开大学 | Based on autofluorescence technology and the classification of the thyroid gland neck tissue of optical coherence tomography and identifying system and method |
| CN213580626U (en) * | 2019-04-04 | 2021-06-29 | 济南显微智能科技有限公司 | Parathyroid gland recognition device |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010014847A1 (en) * | 2008-07-30 | 2010-02-04 | Vanderbilt University | Intra-operative fluorescence spectroscopy and applications of same |
| CN104783760B (en) * | 2015-04-16 | 2017-11-07 | 刘巍巍 | Parathyroid gland identification glasses and its application method in a kind of medical art |
| CN109781701A (en) * | 2019-01-18 | 2019-05-21 | 拉曼兄弟(深圳)科技发展有限公司 | Real-time detection method in a kind of parathyroidectomy based on Raman spectroscopy |
| CN109864715A (en) * | 2019-04-04 | 2019-06-11 | 济南显微智能科技有限公司 | A kind of hand-held human body spontaneous fluorescence detection device |
-
2019
- 2019-08-21 CN CN201910771976.1A patent/CN110558938A/en active Pending
- 2019-11-16 WO PCT/CN2019/119012 patent/WO2020199605A1/en active Application Filing
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203634145U (en) * | 2013-12-31 | 2014-06-11 | 中国人民武装警察部队总医院 | Fluorescence endoscopy imaging system |
| WO2018126114A2 (en) * | 2016-12-29 | 2018-07-05 | Vanderbilt University | Methods and apparatus for intraoperative assessment of parathyroid gland vascularity using laser speckle contrast imaging and applications of same |
| CN107361744A (en) * | 2017-07-31 | 2017-11-21 | 清华大学 | The identification device and method of a kind of parathyroid gland |
| CN108523849A (en) * | 2018-05-02 | 2018-09-14 | 南开大学 | Based on autofluorescence technology and the classification of the thyroid gland neck tissue of optical coherence tomography and identifying system and method |
| CN213580626U (en) * | 2019-04-04 | 2021-06-29 | 济南显微智能科技有限公司 | Parathyroid gland recognition device |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4110167A4 (en) * | 2020-02-25 | 2024-01-24 | Vanderbilt University | Methods of using optical fiber-based fluorescence spectroscopy for surgical guidance and/or tissue diagnostics and applications of same |
| CN112155562A (en) * | 2020-10-20 | 2021-01-01 | 湖南马尼克医疗科技有限公司 | Parathyroid gland composite detection device |
| CN113067240A (en) * | 2021-03-24 | 2021-07-02 | 山东大学 | High repetition frequency self-mode-locking aureoemer laser and application thereof in parathyroid gland identification |
| CN113662515A (en) * | 2021-09-22 | 2021-11-19 | 赛德生物科技(山东)有限责任公司 | Parathyroid gland check out test set |
| WO2023222268A1 (en) * | 2022-05-19 | 2023-11-23 | Fluoptics | Medical imaging device and method suitable for observing a plurality of spectral bands |
| FR3135610A1 (en) * | 2022-05-19 | 2023-11-24 | Fluoptics | Medical imaging device and method adapted to the observation of several spectral bands |
| CN116138744A (en) * | 2023-04-21 | 2023-05-23 | 北京航空航天大学 | Autofluorescence detection probe and system for detecting parathyroid tissue in vivo |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2020199605A1 (en) | 2020-10-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110558938A (en) | Parathyroid gland recognition device and system | |
| CN213580626U (en) | Parathyroid gland recognition device | |
| US8082024B2 (en) | Micro-scale compact device for in vivo medical diagnosis combining optical imaging and point fluorescence spectroscopy | |
| CN105473051B (en) | Medical imaging devices and application method | |
| EP1762183B1 (en) | Lymph node detector | |
| US20100190129A1 (en) | Combination dental hand tool | |
| US20220248944A1 (en) | Modular endoscopic system for visualization of disease | |
| EP3289972A1 (en) | Process and system for intra-operative use of fluorescence and applications of same | |
| CN109310296A (en) | Endoscopic imaging device and method | |
| CN107072505A (en) | Devices, systems, and methods for the drafting of tissue oxygenation | |
| TW201424696A (en) | Diagnostic device, therapeutic device, and uses thereof | |
| EP1720448A1 (en) | Side-firing probe for performing optical spectroscopy during core needle biopsy | |
| CN105962891B (en) | A kind of contact excitation light source of fluorescence imaging detecting system | |
| CN102793533A (en) | Short wave near infrared quantum dot imaging system | |
| WO2002061405A2 (en) | Method and hand-held device for fluorescence detection | |
| US20080306470A1 (en) | Optical screening device | |
| US10746652B1 (en) | Hemoglobin sensor and detecting method thereof | |
| CN1256918C (en) | Laser spectrum cancer diagnostic device with diode laser | |
| CN213249797U (en) | Integrated portable in-situ visible biological target identification and positioning system | |
| CN215272682U (en) | Parathyroid gland position detecting instrument | |
| CN110710953A (en) | Skin tumor early screening device based on fluorescence imaging and use method | |
| CN221383526U (en) | Malignant tumor edge rapid identification system based on autofluorescence lifetime | |
| CN213248999U (en) | Boundary positioning system for in-situ visualization of biological target | |
| CN210221821U (en) | Surface imaging fluorescence spectrum analysis system | |
| RU187072U1 (en) | SAPPHIRE NEUROSURGICAL PROBE FOR TUMOR AND SPINAL TUMOR REMOVAL UNDER CONTROL OF THE COMBINED SPECTROSCOPIC DIAGNOSTICS |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |