CN108523849A - Based on autofluorescence technology and the classification of the thyroid gland neck tissue of optical coherence tomography and identifying system and method - Google Patents
Based on autofluorescence technology and the classification of the thyroid gland neck tissue of optical coherence tomography and identifying system and method Download PDFInfo
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- CN108523849A CN108523849A CN201810407918.6A CN201810407918A CN108523849A CN 108523849 A CN108523849 A CN 108523849A CN 201810407918 A CN201810407918 A CN 201810407918A CN 108523849 A CN108523849 A CN 108523849A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0033—Features or image-related aspects of imaging apparatus classified in A61B5/00, e.g. for MRI, optical tomography or impedance tomography apparatus; arrangements of imaging apparatus in a room
- A61B5/0035—Features or image-related aspects of imaging apparatus classified in A61B5/00, e.g. for MRI, optical tomography or impedance tomography apparatus; arrangements of imaging apparatus in a room adapted for acquisition of images from more than one imaging mode, e.g. combining MRI and optical tomography
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0033—Features or image-related aspects of imaging apparatus classified in A61B5/00, e.g. for MRI, optical tomography or impedance tomography apparatus; arrangements of imaging apparatus in a room
- A61B5/004—Features or image-related aspects of imaging apparatus classified in A61B5/00, e.g. for MRI, optical tomography or impedance tomography apparatus; arrangements of imaging apparatus in a room adapted for image acquisition of a particular organ or body part
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0071—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by measuring fluorescence emission
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0073—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by tomography, i.e. reconstruction of 3D images from 2D projections
Abstract
It is a kind of based on autofluorescence technology and the classification of the thyroid gland neck tissue of optical coherence tomography and identifying system and method.The system is organically merged auto-fluorescence imaging system and optical coherence tomography system, know method for distinguishing by a wide range of coarse positioning and small range essence, using the auto-fluorescence imaging system resolution capability highly sensitive to morphological element and optical coherence tomography system to the high-resolution imaging of institutional framework, the classification and identification of quick, unmarked, lossless, highly sensitive, real-time thyroid gland neck tissue are realized.The present invention can effectively improve doctor in thyroid clinical operation, and, to the classification of the neck tissues such as parathyroid gland, thyroid gland, fat and lymph node and recognition efficiency and accuracy rate, auxiliary doctor protects parathyroid gland, thoroughly cleans lymph node.
Description
Technical field
The present invention relates to a kind of Double-mode imaging system and methods, are based particularly on autofluorescence technology and Optical coherence tomography
The system and method for analysing available Thyreoidine the neck tissue classification and identification of art is that one kind of thyroid gland neck tissue imaging is new
System and method belongs to imaging in biological tissues technical field.
Background technology
In recent years, the incidence of thyroid cancer is in increase trend year by year.Partly or entirely excision is most of evils to thyroid gland
Property and some Benign thyroid diseases optimal selection.Neoplastic lesion recurs in order to prevent, usually to clean the lymph node of surrounding.
In thyroid operation, in order to prevent it is postoperative occur hypocalcemia need protect parathyroid gland.And since parathyroid gland is small
(3-8 millimeters long, 2-5 millimeters of width, 0.5-2 millimeters of thickness), number and position not exclusively determine, and with the lymph node and fat of surrounding
Fat tissue is difficult to distinguish, therefore be easily damaged or mistakenly cut off from the appearance.
In view of two demands of protection parathyroid gland and cleaning lymph node, there is an urgent need for Accurate classifications and knowledge in thyroid operation
Other thyroid gland neck tissue, the i.e. method of parathyroid gland, lymph node and fat.It is clinically existing to classify and identify by first shape
The frozen section method of gland, lymph node and fat and " method of drifting along " there is a problem of losing tissue sample amount and accuracy is not high.It is existing
Some assists in identifying technology such as ultrasonic technique, the fluoroscopic examination based on methylenum careuleum and 5-ALA (ALA), utilizes gamma
The problems such as technologies such as technetium methoxy isonitrile (99mTc-MIBI) imagery of probe are not high or toxic there are resolution ratio.
Based on the method for autofluorescence technology, parathyroid gland and thyroid gland can be excited to generate using the light of 785nm wavelength
Peak value 822nm near-infrared autofluorescence, so as to sort out parathyroid gland and thyroid gland from thyroid gland neck tissue,
And since the autofluorescence of parathyroid gland is higher than thyroid, so as to position parathyroid gland.But near-infrared is spontaneous glimmering
Light technology can not judge whether positioned parathyroid gland is normal parathyroid gland, and can not also classify lymph node and fat.Light
Biological tissue can be realized the structure imaging of high-resolution real non-destructive by learning coherence tomography, in ophthalmology, skin and Cardiological
It is widely used on equal clinical medicines, the classification and identification of different tissues may be implemented.To Thyreoidine neck tissue
Imaging, optical coherence tomography can not quickly position parathyroid gland the problem is that areas imaging is small.
Invention content
Purpose of the present invention is to overcome deficiencies of the prior art, so to thyroid gland neck tissue carry out classification and
Identification, provide it is a kind of based on autofluorescence technology and the thyroid gland neck tissue of optical coherence tomography classification and identifying system and
Method, auxiliary doctor carry out tissue typing and identification in thyroid operation.
The present invention is high the highly sensitive morphological element's resolution capability of near-infrared autofluorescence technology and optical coherence tomography
The structure imaging of resolution combines, and carries out a wide range of coarse positioning and the identification of small range essence, effectively doctor can be assisted to divide during surgery
Class and identification thyroid gland neck tissue.
Technical scheme of the present invention：
It is a kind of based on autofluorescence technology and the thyroid gland neck tissue of optical coherence tomography classification and identifying system, be
A kind of Double-mode imaging system, including be serially connected optical coherence tomography (Optical Coherence Tomography,
OCT) imaging system and autofluorescence (Auto-fluorescence, AF) imaging system, wherein optical coherent chromatographic imaging system
System can be TD-OCT systems, SD-OCT systems either SS-OCT systems.
Include for TD-OCT and AF Double-mode imagings system and SS-OCT and AF Double-mode imaging systems, system hardware：
Wide spectrum light source (1), the first coupler (2), first annular device (3), the first Polarization Controller (4), first collimator (5), first
Speculum (6), the second circulator (7), the second coupler (8), balanced detector (9), the second Polarization Controller (10), the second standard
Straight device (11), the first dichroscope (12), the second speculum (13), scanning galvanometer (14), the first focus lamp (15), sample stage
(16), autofluorescence excitation light source (17), third collimator (18), the second dichroscope (19), photodetector (20)；
Wherein, wide spectrum light source (1), the first coupler (2), first annular device (3), the first Polarization Controller (4), the first standard
Straight device (5), the first speculum (6), the second circulator (7), the second coupler (8), balanced detector (9), the second Polarization Control
Device (10), the second collimator (11), the first dichroscope (12), the second speculum (13), scanning galvanometer (14), the first focus lamp
(15), sample stage (16) constitutes Optical coherence tomography；The light exported from wide spectrum light source (1) is sequentially through the first coupler
(2) first annular device (3) and the second circulator (7) are respectively enterd after being divided, the light being emitted from first annular device (3) passes through first
It is irradiated on the first speculum (6) after Polarization Controller (4), first collimator (5), from the light edge that the first speculum (6) reflects
The first annular device of backtracking (3) enters the ends a of the second coupler (8), and the light being emitted from the second circulator (7) is inclined by second
Shake controller (10), the second collimator (11), the first dichroscope (12), the second speculum (13), scanning galvanometer (14), first
Focus lamp (15) sample on irradiating sample platform (16) afterwards, from the back scattered light of sample along the second circulator of backtracking (7) into
The ends b for entering the second coupler (8), the light into the ends a of the second coupler (8) and the ends b interfere, and the light after interference is from second
The ends c of coupler (8) and d, which are brought out, to be injected into balanced detector (9), and interference signal is acquired by computer control capture card；
For TD-OCT systems, the first speculum (6), which moves longitudinally, realizes sample depth scanning；For SS-OCT systems
System, wide spectrum light source (1) realize sample depth scanning using frequency sweep wide spectrum light source；
It is spontaneous glimmering for TD-OCT technologies and AF Double-mode imagings system and SS-OCT technologies and AF Double-mode imaging systems
Phot-luminescence source (17), third collimator (18), the second dichroscope (19), photodetector (20), the first dichroscope
(12), the second speculum (13), scanning galvanometer (14), the first focus lamp (15), sample stage (16) constitute auto-fluorescence imaging system
System；The light exported from autofluorescence excitation light source (17), sequentially through third collimator (18), the second dichroscope (19), the
It is radiated on sample stage (16) after one dichroscope (12), the second speculum (13), scanning galvanometer (14), the first focus lamp (15)
Sample on excite autofluorescence, the autofluorescence being reflected back by the first focus lamp (15), scanning galvanometer (14), second reflection
It is reflected on the first dichroscope (12) after mirror (13), then photodetector is reflected on the second dichroscope (19)
(20), auto flourescence signals are acquired by computer control capture card.
For SD-OCT and AF Double-mode imaging systems, system hardware includes：Wide spectrum light source (1), the first coupler (2),
First Polarization Controller (4), first collimator (5), the first speculum (6), the 4th collimator (21), grating (22), second gather
Burnt mirror (23), line-scan digital camera (24), the second Polarization Controller (10), the second collimator (11), the first dichroscope (12), second
Speculum (13), scanning galvanometer (14), the first focus lamp (15), sample stage (16), autofluorescence excitation light source (17), third are accurate
Straight device (18), the second dichroscope (19), photodetector (20)；
For SD-OCT technologies and AF Double-mode imaging systems, wherein wide spectrum light source (1), the first coupler (2), first
Polarization Controller (4), first collimator (5), the first speculum (6), the 4th collimator (21), grating (22), the second focus lamp
(23), line-scan digital camera (24), the second Polarization Controller (10), the second collimator (11), the first dichroscope (12), the second reflection
Mirror (13), scanning galvanometer (14), the first focus lamp (15), sample stage (16) constitute spectral domain optical coherent tomographic system；From wide range
The light of light source (1) output enters the ends a of the first coupler (2), is respectively enterd from the ends b of the first coupler (2) and the ends c after light splitting
First Polarization Controller (4) and the second Polarization Controller (10), the light being emitted from the first Polarization Controller (4) is by the first collimation
It is irradiated to after device (5) on the first speculum (6), the light reflected from the first speculum (6) enters the second coupler along backtracking
(2) the ends b；The light being emitted from the second Polarization Controller (10) is by the second collimator (11), the first dichroscope (12), second
Speculum (13), scanning galvanometer (14), the first focus lamp (15) sample on irradiating sample platform (16) afterwards, it is back scattered from sample
Light enters the ends c of the first coupler (2) along backtracking；Light into the ends b of the first coupler (2) and the ends c interferes, and does
Light after relating to brought out from the d of the first coupler (2) inject into the 4th collimator (21), grating (22), the second focus lamp (23) and
Line-scan digital camera (24) acquires interference signal by computer control capture card；
For SD-OCT systems and AF Double-mode imaging systems, autofluorescence excitation light source (17), third collimator (18),
Second dichroscope (19), photodetector (20), the first dichroscope (12), the second speculum (13), scanning galvanometer (14),
First focus lamp (15), sample stage (16) constitute auto-fluorescence imaging system；The light exported from autofluorescence excitation light source (17),
Sequentially through third collimator (18), the second dichroscope (19), the first dichroscope (12), the second speculum (13), scanning
Autofluorescence is excited on the sample being radiated on sample stage (16) after galvanometer (14), the first focus lamp (15), what is be reflected back is spontaneous
Fluorescence reflects after the first focus lamp (15), scanning galvanometer (14), the second speculum (13) on the first dichroscope (12),
Photodetector (20) is reflected on the second dichroscope (19) again, by computer control capture card acquisition autofluorescence letter
Number.
The optical coherence tomography system and auto-fluorescence imaging system utilizes the first dichroscope (12), second
Speculum (13), scanning galvanometer (14), the first focus lamp (15) and sample stage (16) are organically merged.
Wave band is in 1300nm or 1550nm used by the light source of the Optical coherence tomography.
Autofluorescence excitation light source used by the auto-fluorescence imaging system may be used 780nm LED,
785nm lasers or the other light sources for covering 780nm.
First dichroscope (12) is the dichroscope and the second dichroscope (19) of long wave transmission shortwave reflection
It is the dichroscope that shortwave transmits long wave reflection.
It is provided by the invention to classify and know based on autofluorescence technology and the thyroid gland neck tissue of optical coherence tomography
The recognition methods of other system knows method for distinguishing using coarse scanning and essence and carries out parathyroid gland positioning and identification, i.e., first with certainly
Technical controlling scanning galvanometer (14) fluoresce in parathyroid gland hot-zone large area scanning, it is determined whether there are auto flourescence signals, when
Determine that there are areas in 8mm2~32mm2And auto flourescence signals are better than the region of the auto flourescence signals of sample around
When, then small range scanning is carried out in the region using Optical coherence tomography control scanning galvanometer (14), acquire the region
Optical coherence tomography image, further confirms that whether the tissue in the region is parathyroid gland and identifies whether as normal first shape
Other gland.For other tissues that removed doctor in operation can not confirm, it is scanned into using Optical coherence tomography
Picture classifies and identifies that the tissue is thyroid gland, parathyroid gland, fat or lymph node.
The auto-fluorescence imaging system uses photodetector, acquires auto flourescence signals, controls scanning galvanometer
(14) scanning obtains two-dimentional autofluorescence image.
The advantages of the present invention：
The present invention is based on classification and identification that autofluorescence technology and optical coherence tomography realize thyroid gland neck tissue,
Autofluorescence technology and optical coherence tomography are organically merged, identified by a wide range of coarse positioning and small range essence
Method, using the autofluorescence technology resolution capability highly sensitive to morphological element and optical coherence tomography to institutional framework
High-resolution imaging realizes the classification and identification of quick, unmarked, lossless, highly sensitive, real-time thyroid gland neck tissue.Can have
Effect improves classification and knowledge of the doctor to neck tissues such as parathyroid gland, thyroid gland, fat and lymph nodes in thyroid clinical operation
Other efficiency and accuracy rate, auxiliary doctor protect parathyroid gland, thoroughly clean lymph node.
Description of the drawings
Fig. 1 is TD-OCT and AF Double-mode imagings system and SS-OCT and AF Double-mode imaging system composition schematic diagrams；
Fig. 2 is SDOCT-AF Double-mode imaging system composition schematic diagrams；
Fig. 3 is scanning range schematic diagram；
In figure, 1. wide spectrum light sources, 2. first couplers, 3. first annular devices, 4. first Polarization Controllers, 5. first collimations
Device, 6. first speculums, 7. second circulators, 8. second couplers, 9. balanced detectors, 10. second Polarization Controllers, 11.
Second collimator, 12. first dichroscopes, 13. second speculums, 14. scanning galvanometers, 15. first focus lamps, 16. sample stages,
17. autofluorescence excitation light source, 18. third collimators, 19. second dichroscopes, 20. photodetectors；
21 the 4th collimators, 22 gratings, 23 second focus lamps, 24 line-scan digital cameras；
25. thyroid gland, 26. parathyroid glands, 27. autofluorescence system scanning areas, 28. Optical coherence tomographies scan
Region.
Specific implementation mode
The specific implementation mode further illustrated the present invention below in conjunction with the accompanying drawings.
Embodiment 1：
Include for TD-OCT and AF Double-mode imagings system and SS-OCT and AF Double-mode imaging systems, system hardware：
The wide spectrum light source (1) of 1300nm or 1550nm, the first coupler (2), first annular device (3), the first Polarization Controller (4),
Collimator (5), the first speculum (6), the second circulator (7), the second coupler (8), balanced detector (9), the second polarization
Controller (10), the second collimator (11), the first dichroscope (12), the second speculum (13), scanning galvanometer (14), first gather
Burnt mirror (15), sample stage (16), using 780nm LED or 785nm lasers or cover 780nm other light sources as spontaneous
Fluorescence excitation light source (17), third collimator (18), the second dichroscope (19), photodetector (20)；
Wherein, wide spectrum light source (1), the first coupler (2), first annular device (3), the first Polarization Controller (4), the first standard
Straight device (5), the first speculum (6), the second circulator (7), the second coupler (8), balanced detector (9), the second Polarization Control
Device (10), the second collimator (11), the first dichroscope (12), the second speculum (13), scanning galvanometer (14), the first focus lamp
(15), sample stage (16) constitutes Optical coherence tomography；The light exported from wide spectrum light source (1) is sequentially through the first coupler
(2) first annular device (3) and the second circulator (7) are respectively enterd after being divided, the light being emitted from first annular device (3) passes through first
It is irradiated on the first speculum (6) after Polarization Controller (4), first collimator (5), from the light edge that the first speculum (6) reflects
The first annular device of backtracking (3) enters the ends a of the second coupler (8), and the light being emitted from the second circulator (7) is inclined by second
Shake controller (10), the second collimator (11), the first dichroscope (12), the second speculum (13), scanning galvanometer (14), first
Focus lamp (15) sample on irradiating sample platform (16) afterwards, from the back scattered light of sample along the second circulator of backtracking (7) into
The ends b for entering the second coupler (8), the light into the ends a of the second coupler (8) and the ends b interfere, and the light after interference is from second
The ends c of coupler (8) and d, which are brought out, to be injected into balanced detector (9), and interference signal is acquired by computer control capture card；
For TD-OCT systems, the first speculum (6), which moves longitudinally, realizes sample depth scanning；For SS-OCT systems
System, wide spectrum light source (1) realize sample depth scanning using frequency sweep wide spectrum light source；
Autofluorescence excitation light source (17), third collimator (18), the second dichroscope (19), photodetector (20),
First dichroscope (12), the second speculum (13), scanning galvanometer (14), the first focus lamp (15), sample stage (16) are constituted certainly
Fluoresce imaging system；From autofluorescence excitation light source (17) export light, sequentially through third collimator (18), the two or two to
Look mirror (19), the first dichroscope (12), the second speculum (13), scanning galvanometer (14), the first focus lamp are radiated at after (15)
Autofluorescence is excited on sample on sample stage (16), the autofluorescence being reflected back is by the first focus lamp (15), scanning galvanometer
(14), it reflects on the first dichroscope (12) after the second speculum (13), then is reflected on the second dichroscope (19)
Photodetector (20) acquires auto flourescence signals by computer control capture card.
Above-mentioned first dichroscope (12) is the dichroscope of long wave transmission shortwave reflection and the second dichroscope (19) is
Shortwave transmits the dichroscope of long wave reflection.
When specific scanning, first with AF technical controllings scanning galvanometer (14) in parathyroid gland hot-zone, that is, autofluorescence system
A wide range of scanning (as shown in Fig. 3) in scanning area (27), it is determined whether have near-infrared of the peak value near 822nm spontaneous glimmering
Optical signal.When being detected using photodetector (20), there are areas in 8mm2~32mm2And the auto flourescence signals are strong
When the region of the auto flourescence signals of sample around, it is small in region progress to reuse OCT systems control scanning galvanometer (14)
(as shown in Fig. 3) is scanned in range, that is, Optical coherence tomography scanning area (28), acquires the Optical coherence tomography in the region
Image is analysed, further confirms that whether the tissue in the region is parathyroid gland (26) and identifies whether as normal parathyroid gland.It is right
Other tissues that removed doctor can not confirm in operation are scanned imaging using OCT systems, classify and identify the group
Knit is thyroid gland (25), parathyroid gland (26), fat or lymph node.
Embodiment 2：
For SD-OCT and AF Double-mode imaging systems, system hardware includes：The wide spectrum light source of 1300nm or 1550nm
(1), the first coupler (2), the first Polarization Controller (4), first collimator (5), the first speculum (6), the 4th collimator
(21), grating (22), the second focus lamp (23), line-scan digital camera (24), the second Polarization Controller (10), the second collimator (11),
First dichroscope (12), scanning galvanometer (14), the first focus lamp (15), sample stage (16), uses the second speculum (13)
LED the or 785nm lasers of 780nm or the other light sources for covering 780nm are collimated as autofluorescence excitation light source (17), third
Device (18), the second dichroscope (19), photodetector (20)；
Wherein, wide spectrum light source (1), the first coupler (2), the first Polarization Controller (4), first collimator (5), first anti-
Penetrate mirror (6), the 4th collimator (21), grating (22), the second focus lamp (23), line-scan digital camera (24), the second Polarization Controller
(10), the second collimator (11), the first dichroscope (12), the second speculum (13), scanning galvanometer (14), the first focus lamp
(15), sample stage (16) constitutes SD-OCT systems；The light exported from wide spectrum light source (1) enters the ends a of the first coupler (2), point
The first Polarization Controller (4) and the second Polarization Controller (10) are respectively enterd from the ends b of the first coupler (2) and the ends c after light, from
The light of first Polarization Controller (4) outgoing is irradiated to after first collimator (5) on the first speculum (6), from the first reflection
The light of mirror (6) reflection enters the ends b of the second coupler (2) along backtracking；The light warp being emitted from the second Polarization Controller (10)
Cross the second collimator (11), the first dichroscope (12), the second speculum (13), scanning galvanometer (14), the first focus lamp (15)
Sample on irradiating sample platform (16) afterwards enters the ends c of the first coupler (2) from the back scattered light of sample along backtracking；Into
The light at the ends b and the ends c that enter the first coupler (2) interferes, the light after interference brought out from the d of the first coupler (2) inject into
4th collimator (21), grating (22), the second focus lamp (23) and line-scan digital camera (24), it is dry by computer control capture card acquisition
Relate to signal；
Autofluorescence excitation light source (17), third collimator (18), the second dichroscope (19), photodetector (20),
First dichroscope (12), the second speculum (13), scanning galvanometer (14), the first focus lamp (15), sample stage (16) are constituted certainly
Fluoresce imaging system；From autofluorescence excitation light source (17) export light, sequentially through third collimator (18), the two or two to
Look mirror (19), the first dichroscope (12), the second speculum (13), scanning galvanometer (14), the first focus lamp are radiated at after (15)
Autofluorescence is excited on sample on sample stage (16), the autofluorescence being reflected back is by the first focus lamp (15), scanning galvanometer
(14), it reflects on the first dichroscope (12) after the second speculum (13), then is reflected on the second dichroscope (19)
Photodetector (20) acquires auto flourescence signals by computer control capture card.
Above-mentioned first dichroscope (12) is the dichroscope of long wave transmission shortwave reflection and the second dichroscope (19) is
Shortwave transmits the dichroscope of long wave reflection.
When specific scanning, first with autofluorescence technical controlling scanning galvanometer (14) in parathyroid gland hot-zone, that is, spontaneous glimmering
A wide range of scanning (as shown in Fig. 3) in photosystem scanning area (27), it is determined whether have near-infrared of the peak value near 822nm
Auto flourescence signals.When being detected using photodetector (20), there are areas in 8mm2~32mm2And the autofluorescence
When signal is better than the region of the auto flourescence signals of sample around, then using Optical coherence tomography control scanning galvanometer (14)
It carries out scanning (as shown in Fig. 3) in small range, that is, Optical coherence tomography scanning area (28) in the region, acquires the area
The optical coherence tomography image in domain, further confirms that whether the tissue in the region is parathyroid gland (26) and identifies whether as just
Normal parathyroid gland.For other tissues that removed doctor in operation can not confirm, using Optical coherence tomography into
Row scanning imagery classifies and identifies that the tissue is thyroid gland (25), parathyroid gland (26), fat or lymph node.
Claims (8)
1. being existed based on autofluorescence technology and the classification of the thyroid gland neck tissue of optical coherence tomography and identifying system, feature
In the system is a kind of Double-mode imaging system, including the optical coherence tomography (Optical being serially connected
Coherence Tomography, OCT) imaging system and autofluorescence (Auto-fluorescence, AF) imaging system,
In, optical coherence tomography system is Time Domain Optical coherent tomographic (Time Domain OCT, TD-OCT) system, spectral domain light
Learn coherent tomographic (Spectral-domain OCT, SD-OCT) system either swept light source optical coherence tomography (Swept
Source OCT, SS-OCT) system；
Include for TD-OCT and AF Double-mode imagings system and SS-OCT and AF Double-mode imaging systems, system hardware：Wide range
Light source (1), the first coupler (2), first annular device (3), the first Polarization Controller (4), first collimator (5), the first reflection
Mirror (6), the second circulator (7), the second coupler (8), balanced detector (9), the second Polarization Controller (10), the second collimator
(11), the first dichroscope (12), the second speculum (13), scanning galvanometer (14), the first focus lamp (15), sample stage (16),
Autofluorescence excitation light source (17), third collimator (18), the second dichroscope (19), photodetector (20)；
Wherein, wide spectrum light source (1), the first coupler (2), first annular device (3), the first Polarization Controller (4), first collimator
(5), the first speculum (6), the second circulator (7), the second coupler (8), balanced detector (9), the second Polarization Controller
(10), the second collimator (11), the first dichroscope (12), the second speculum (13), scanning galvanometer (14), the first focus lamp
(15), sample stage (16) constitutes Optical coherence tomography；The light exported from wide spectrum light source (1) is sequentially through the first coupler
(2) first annular device (3) and the second circulator (7) are respectively enterd after being divided, the light being emitted from first annular device (3) passes through first
It is irradiated on the first speculum (6) after Polarization Controller (4), first collimator (5), from the light edge that the first speculum (6) reflects
The first annular device of backtracking (3) enters the ends a of the second coupler (8), and the light being emitted from the second circulator (7) is inclined by second
Shake controller (10), the second collimator (11), the first dichroscope (12), the second speculum (13), scanning galvanometer (14), first
Focus lamp (15) sample on irradiating sample platform (16) afterwards, from the back scattered light of sample along the second circulator of backtracking (7) into
The ends b for entering the second coupler (8), the light into the ends a of the second coupler (8) and the ends b interfere, and the light after interference is from second
The ends c of coupler (8) and d, which are brought out, to be injected into balanced detector (9), and interference signal is acquired by computer control capture card；
For TD-OCT systems, the first speculum (6), which moves longitudinally, realizes sample depth scanning；It is wide for SS-OCT systems
It composes light source (1) and sample depth scanning is realized using frequency sweep wide spectrum light source；
Autofluorescence excitation light source (17), third collimator (18), the second dichroscope (19), photodetector (20), first
Dichroscope (12), the second speculum (13), scanning galvanometer (14), the first focus lamp (15), sample stage (16) constitute spontaneous glimmering
Photoimaging systems；The light exported from autofluorescence excitation light source (17), sequentially through third collimator (18), the second dichroscope
(19), the first dichroscope (12), the second speculum (13), scanning galvanometer (14), the first focus lamp are radiated at sample after (15)
Excite autofluorescence on sample on platform (16), the autofluorescence being reflected back by the first focus lamp (15), scanning galvanometer (14),
It is reflected on the first dichroscope (12) after second speculum (13), then is reflected into photoelectricity spy on the second dichroscope (19)
Device (20) is surveyed, auto flourescence signals are acquired by computer control capture card.
2. according to claim 1 classified based on autofluorescence technology and the thyroid gland neck tissue of optical coherence tomography
And identifying system, which is characterized in that for SD-OCT and AF Double-mode imaging systems, system hardware includes：Wide spectrum light source (1),
First coupler (2), the first Polarization Controller (4), first collimator (5), the first speculum (6), the 4th collimator (21), light
Grid (22), the second focus lamp (23), line-scan digital camera (24), the second Polarization Controller (10), the second collimator (11), the one or two to
Look mirror (12), the second speculum (13), scanning galvanometer (14), the first focus lamp (15), sample stage (16), autofluorescence exciting light
Source (17), third collimator (18), the second dichroscope (19), photodetector (20)；
Wherein, wide spectrum light source (1), the first coupler (2), the first Polarization Controller (4), first collimator (5), the first speculum
(6), the 4th collimator (21), grating (22), the second focus lamp (23), line-scan digital camera (24), the second Polarization Controller (10),
Two collimators (11), the first dichroscope (12), the second speculum (13), scanning galvanometer (14), the first focus lamp (15), sample
Platform (16) constitutes SD-OCT systems；The light exported from wide spectrum light source (1) enters the ends a of the first coupler (2), from first after light splitting
The ends b and the ends c of coupler (2) respectively enter the first Polarization Controller (4) and the second Polarization Controller (10), from the first polarization control
The light of device (4) outgoing processed is irradiated to after first collimator (5) on the first speculum (6), is reflected from the first speculum (6)
Light enters the ends b of the second coupler (2) along backtracking；The light being emitted from the second Polarization Controller (10) passes through the second collimator
(11), the first dichroscope (12), the second speculum (13), scanning galvanometer (14), the first focus lamp (15) irradiating sample platform afterwards
(16) sample on enters the ends c of the first coupler (2) from the back scattered light of sample along backtracking；Into the first coupler
(2) light at the ends b and the ends c interferes, and the light after interference brings out from the d of the first coupler (2) and injects into the 4th collimator
(21), grating (22), the second focus lamp (23) and line-scan digital camera (24) acquire interference signal by computer control capture card；
Autofluorescence excitation light source (17), third collimator (18), the second dichroscope (19), photodetector (20), first
Dichroscope (12), the second speculum (13), scanning galvanometer (14), the first focus lamp (15), sample stage (16) constitute spontaneous glimmering
Photoimaging systems；The light exported from autofluorescence excitation light source (17), sequentially through third collimator (18), the second dichroscope
(19), the first dichroscope (12), the second speculum (13), scanning galvanometer (14), the first focus lamp are radiated at sample after (15)
Excite autofluorescence on sample on platform (16), the autofluorescence being reflected back by the first focus lamp (15), scanning galvanometer (14),
It is reflected on the first dichroscope (12) after second speculum (13), then is reflected into photoelectricity spy on the second dichroscope (19)
Device (20) is surveyed, auto flourescence signals are acquired by computer control capture card.
3. the thyroid gland neck tissue according to claim 1 or 2 based on autofluorescence technology and optical coherence tomography
Classification and identifying system, which is characterized in that the Double-mode imaging system, i.e. OCT systems and AF imaging systems utilize first
Dichroscope (12), the second speculum (13), scanning galvanometer (14), the first focus lamp (15) and sample stage (16) organically merge
Together.
4. the thyroid gland neck tissue according to claim 1 or 2 based on autofluorescence technology and optical coherence tomography
Classification and identifying system, which is characterized in that wave band used by the wide spectrum light source of the OCT systems in 1300nm or
1550nm。
5. the thyroid gland neck tissue according to claim 1 or 2 based on autofluorescence technology and optical coherence tomography
Classification and identifying system, which is characterized in that autofluorescence excitation light source used by the AF imaging systems is using 780nm's
LED, 785nm laser or the other light sources for covering 780nm.
6. the thyroid gland neck tissue according to claim 1 or 2 based on autofluorescence technology and optical coherence tomography
Classification and identifying system, which is characterized in that first dichroscope (12) is the dichroscope of long wave transmission shortwave reflection
It is the dichroscope that shortwave transmits long wave reflection with the second dichroscope (19).
7. carrying out the classification of thyroid gland neck tissue based on system as claimed in claim 1 or 2 and knowing method for distinguishing, feature exists
In knowing method for distinguishing using coarse scanning and essence and carry out parathyroid gland positioning and identification, i.e., shake first with the control scanning of AF systems
Mirror (14) is in parathyroid gland hot-zone large area scanning, it is determined whether has auto flourescence signals, when there are areas in 8mm for determination2
~32mm2And when AF signals are better than the region of AF signals of sample around, then using OCT systems control scanning galvanometer (14)
Small range scanning is carried out in the region, the OCT image in the region is acquired, further confirms that whether the tissue in the region is by first shape
Gland simultaneously identifies whether as normal parathyroid gland；For other tissues that removed doctor in operation can not confirm, use
OCT systems are scanned imaging, classify and identify that the tissue is thyroid gland, parathyroid gland, fat or lymph node.
8. thyroid gland neck tissue classification according to claim 7 and knowledge method for distinguishing, which is characterized in that AF systems use
Photodetector acquires AF strength signals, and control scanning galvanometer (14), which scans, obtains two dimension AF intensity images.
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