CN110548078A

CN110548078A - Composition containing Rutaceae plant extract

Info

Publication number: CN110548078A
Application number: CN201811022911.9A
Authority: CN
Inventors: 张仁维
Original assignee: Individual
Current assignee: Individual
Priority date: 2018-05-31
Filing date: 2018-09-03
Publication date: 2019-12-10
Also published as: TW202003007A; TWI731244B

Abstract

The invention provides a composition containing Rutaceae plant extract, which comprises 0.1-2 wt% of Rutaceae plant extract, 10-30 wt% of C1-C4 polyalcohol system and 60-70 wt% of pure water. The composition containing the Rutaceae plant extract can resist bacteria and viruses in a short time, has lubricating and moisturizing effects, and is sanitary, safe and good in using effect. The invention has the beneficial effects of lubrication, moisture retention and the like, and has the effects of short-time antibiosis, virus resistance, disease infection prevention and the like.

Description

Composition containing Rutaceae plant extract

Technical Field

The invention relates to the field of Rutaceae plant extracts, and relates to a composition containing Rutaceae plant extracts.

Background

Natural acidic lubricating fluids normally exist in the vagina and are produced in increments when making love. For several reasons, including decreased estrogen during menopause, or following ovariectomy, and after radiation therapy, the lubricant may decrease, causing vaginal tissue to become dry and irritated. This can lead to pain and/or bleeding when making love. Oral contraceptives and certain drugs such as antihistamines, antidepressants, hypotensives and heart drugs also cause vaginal dryness. Additionally, psychological conditions, including stress, exertion, and anxiety, may hinder the production of natural lubricating fluids. After delivery, especially when the mother is nursing in person, the combination of hormones and psychological factors may induce a temporary dryness of the vagina.

Common personal lubricants or care and cleansing products, such as silicones and commercially available K-Y gels and petrolatum, are based primarily on synthetic materials such as silicones, petrochemical materials. Thus, such commercially available acidic vaginal lubricants are not recommended for use by women who want to become pregnant, or by groups who focus on natural organic, preventive care.

Disclosure of Invention

An object of the present invention is to provide a composition containing an extract of a rutaceae plant, which has not only a health-care effect of lubrication, moisture retention, etc., but also an antibacterial, antiviral, disease infection prevention, etc. effect for a short time.

In order to achieve the above objects, the present invention provides a composition containing rutaceae plant extract, comprising 0.1-2 wt% of rutaceae plant extract, 10-30 wt% of C1-C4 polyol system, and 60-70 wt% of pure water. In one aspect, the rutaceae plant extract-containing composition of the present invention comprises 0.1-2 wt% of rutaceae plant extract, 2-10 wt% of C5-C8 polyol system, and 60-70 wt% of pure water. In one aspect, the rutaceae plant extract is an extract selected from any one of the group consisting of oranges, mandarins, sweet oranges, lime, bitter oranges, pomelos, grapefruits, lemons, limes, lemons, bergamots, and kumquats. The polyol system is a composition selected from any one of the group consisting of glycerol, propylene glycol, methyl propylene glycol, butylene glycol, pentylene glycol, hexylene glycol, octylene glycol, and ethylhexylglycerin.

In order to achieve the above objects, the present invention provides another composition containing extracts of rutaceae plants, comprising 0.1 to 2 wt% of extracts of rutaceae plants; 10-30 wt% of a C1-C4 polyol system; 1-4 wt% of a natural preservative; 0.5-2 wt% of natural thickening agent and 60-70 wt% of pure water. In one aspect, the present invention relates to another composition comprising an extract of a rutaceae plant, comprising 0.1 to 2 wt% of the extract of the rutaceae plant; 2-10 wt% of a C5-C8 polyol system; 1-4 wt% of a natural preservative; 1-3 wt% of natural thickening agent and 60-70 wt% of pure water.

In one aspect, the rutaceae plant extract is an extract selected from any one of the group consisting of oranges, mandarins, sweet oranges, lime, bitter oranges, pomelos, grapefruits, lemons, limes, lemons, bergamots, and kumquats.

The C1-C4 polyol system is a composition selected from any one of the group consisting of glycerol, propylene glycol, methyl propylene glycol, and butylene glycol. The C5-C8 polyol system is a composition selected from any one of the group consisting of pentanediol, hexanediol, octanediol, and octanediol. The natural thickener is a composition selected from any one of the group consisting of xanthan gum, guar gum, sodium alginate and gum arabic. Depending on the purpose of use, non-natural thickeners may also be used.

The preservatives are, for example, sodium anisate and sodium levulinate; octanoyl hydroxamic acid and lactic acid; or glyceryl caprylate and glyceryl undecylenate. Suitable preservatives of the present invention may be acid preservatives such as hexadienoic acid, anhydrous acetic acid, benzoic acid, p-hydroxybenzoic acid or salicylic acid. Alternatively, the preservative of the present invention may be a paraben based preservative, such as methyl paraben, ethyl paraben, propyl paraben, butyl paraben, isopropyl paraben, isobutyl paraben or second butyl paraben. In addition, the preservative of the present invention may be methylisothiazolinone, methylchloroisothiazolinone ketone, iodopropynyl butylcarbamate, phenoxyethanol, chlorphenesin, triclosan, quaternary ammonium salts or cationic surfactants.

According to the composition containing the Rutaceae plant extract, hyaluronic acid powder, aloe extract, essential oil or essence and other anti-aging or whitening functional raw materials can be additionally added according to requirements. The composition containing the Rutaceae plant extract can resist bacteria and viruses in a short time, has lubricating and moisturizing effects, and is sanitary and safe; the use effect is good. In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below. It is to be noted that the components in the attached drawings are merely schematic and are not shown in actual scale.

Drawings

FIG. 1 shows an aqueous solution of a pure Rutaceae plant extract (sample 1); polyol system, preservative and natural thickener (sample 2); and aqueous extracts of rutaceae plants, polyol systems, preservatives and natural thickeners (sample 3), respectively, for comparison against the HIV-1 virus test.

FIG. 2 is a graph showing the utilization of an 80-fold dilution of an aqueous solution of a pure Rutaceae plant extract (sample 1); polyol system, preservative and natural thickener (sample 2); and aqueous extracts of rutaceae plants, polyol systems, preservatives and natural thickeners (sample 3), respectively, to inhibit HIV-1 infection of jlrg CD4+ cells.

Detailed Description

One embodiment of the present invention relates to a composition containing Rutaceae plant extract, comprising 0.1-2 wt% of Rutaceae plant extract, 10-30 wt% of C1-C4 polyalcohol system and 60-70 wt% of pure water. In another embodiment, a composition comprising extracts of rutaceae plants comprises 0.1-2 wt% of rutaceae plant extracts, 2-10 wt% of a C5-C8 polyol system, and 60-70 wt% of purified water. In one embodiment, the rutaceae plant extract is an extract selected from any one of the group consisting of oranges, mandarins, sweet oranges, lime, bitter oranges, pomelos, grapefruits, lemons, limes, lemons, bergamots, and kumquats. The Rutaceae plant extract can open cell membrane of bacteria, allow other substances to enter, and enhance sterilization. In addition, the Rutaceae plant extract also has antiviral effect. The Rutaceae plant extract also has antibacterial and antiviral effects.

The C1-C4 polyol system is a composition selected from any one of the group consisting of glycerol, propylene glycol, methyl propylene glycol, and butylene glycol. The C5-C8 polyol system is a composition selected from any one of the group consisting of pentanediol, hexanediol, octanediol, and octanediol. The above polyols, such as propylene glycol, have a lubricating effect. The glycerin can absorb moisture in the air to moisturize the skin, can lock the moisture in the skin and prevent the moisture from losing, and has the moisturizing effect. In addition, these polyols all have the effect of "self-preservation", which can increase the antibacterial power and enhance the disinfection effect.

In another embodiment, the invention relates to a composition comprising extracts of rutaceae plants, comprising 0.1-2 wt% of rutaceae plant extracts; 10-30 wt% of a C1-C4 polyol system; 1-4 wt% of a preservative; 0.5-2 wt% of natural thickener and 60-70 wt% of pure water. In another embodiment, the rutaceae plant extract-containing composition of the present invention comprises 0.1 to 2 wt% of a rutaceae plant extract; 2-10 wt% of a C5-C8 polyol system; 1-4 wt% of a preservative; 1-3 wt% of natural thickener and 60-70 wt% of pure water. In one embodiment, the rutaceae plant extract is an extract selected from any one of the group consisting of oranges, mandarins, sweet oranges, lime, bitter oranges, pomelos, grapefruits, lemons, limes, lemons, bergamots, and kumquats. The Rutaceae plant extract can open cell membrane of bacteria, allow other substances to enter, and enhance sterilization. In addition, the Rutaceae plant extract also has antiviral effect.

The natural thickener is a composition selected from any one of the group consisting of xanthan gum, guar gum, sodium alginate and gum arabic. Depending on the purpose of use, non-natural thickeners may also be used.

The preservatives are, for example, sodium anisate and sodium levulinate; octanoyl hydroxamic acid and lactic acid; or glyceryl caprylate and glyceryl undecylenate. Suitable preservatives of the present invention may also be acid preservatives, for example hexadienoic acid, anhydrous acetic acid, benzoic acid, p-hydroxybenzoic acid or salicylic acid. Alternatively, the preservative of the present invention may also be a paraben preservative, such as methyl paraben, ethyl paraben, propyl paraben, butyl paraben, isopropyl paraben, isobutyl paraben or second butyl paraben. Further, suitable preservatives of the present invention may also be methylisothiazolinone, methylchloroisothiazolinone ketone, iodopropynyl butylcarbamate, phenoxyethanol, chlorphenesin, triclosan, quaternary ammonium salts or cationic surfactants.

According to the composition containing the Rutaceae plant extract, other auxiliary raw materials such as hyaluronic acid powder, aloe extract or essential oil and the like can be additionally added according to requirements, so that the antibacterial and antiviral effects are not influenced. The composition containing the Rutaceae plant extract can resist bacteria and viruses in a short time, has lubricating and moisturizing effects, and is sanitary and safe; the use effect is good.

Example 1: preparing aqueous solution of Rutaceae plant extract

In this example, three aqueous solutions of rutaceae plant extracts at different concentrations and pH values were used for the antibacterial activity test, with 0.5 v% and pH 2.5, 1 v% and pH 4.4 and 1 v% and pH 5.2, and the results of the test data are shown in table 1.

TABLE 1

As a result of the test data shown in Table 1, it was found that an aqueous solution of a Rutaceae plant extract having a concentration of 0.5 v% and a pH of 2.5 has a superior antibacterial activity, and that the antibacterial activity cannot be effectively improved if the concentration is increased to 1 v% while the pH must be increased in order to reduce the irritation of the vagina or the use of normal human skin by the composition containing the Rutaceae plant extract according to the present invention. The pH value of a common commercially available human lubricant product is 5-7, and according to detection data, the antibacterial power at pH 5.2 is the worst of three conditions, and the antibacterial power can not reach 99.9% of threshold within 20 minutes. According to the normal smearing and using time of the human body lubricant, the antibacterial power of 99.9 percent must be achieved by setting 20 minutes generally. The rutaceae plant extract diluted with water can have a general antibacterial activity at 0.5 v% and a pH of 2.5, but if the pH is within a general use range (pH 4-6) of a commercially available product, the antibacterial activity is significantly reduced, and even if the concentration is increased to 1 v%, the antibacterial activity is only 30-40%. Therefore, when the aqueous solution of the extract of the rutaceae plant is used and the pH is in the range of the general commercial product (pH 4-6), the antibacterial power is obviously insufficient.

Example 2: adding natural thickener into aqueous solution of Rutaceae plant extract

This example mixes a 1.55 wt% aqueous solution of a rutaceae plant extract with xanthan gum (0.4 wt%), guar gum (0.6 wt%), hyaluronic acid powder (0.05 wt%), aloe vera extract (0.1 wt%), vegetable glycerin (9.5%) and purified water to form a gel. The above-mentioned materials are basically natural materials which are easy to grow bacteria, except glycerin. The results of the test data are shown in table 2.

TABLE 2

The detection data results shown in table 2 show that the antibacterial power is obviously reduced or no antibacterial power, and the aqueous solution of the rutaceae plant extract cannot be used as the antibacterial and antiseptic of gel products, and even more specifically, the short-time sterilization and virus killing effect can be achieved. Therefore, when the aqueous solution of the extract of the rutaceae plant and the natural thickening agent which is easy to grow bacteria and not easy to store for a long time, such as xanthan gum, guar gum and the like, are mixed to prepare the gel, the result of the detection data shows that the antibacterial power is obviously insufficient, and even the bacteria resistance and the corrosion resistance are doubtful.

Example 3: adding aqueous solution of extract of Rutaceae plant into water and polyalcohol containing modified silicone oil as base

In this example, a 1 wt% aqueous solution of a rutaceae plant extract was added to a mixture of 80 wt% modified silicone oil of Dow Corning 2501, 9 wt% water and 10 wt% glycerin, the pH of the formulation was 4.5, and the results of the measurement data are shown in table 3.

TABLE 3

The detection data in table 3 show that 99.9% of antibacterial activity can be achieved in 20 minutes, which may be caused by the synergistic effect of the polyols on the effectiveness of the rutaceae plant extract or the synergistic effect of the polyols on the effectiveness of the rutaceae plant extract; and the rutaceae plant extract can not reach 99.9 percent of antibacterial effect under the condition that water is used as a substrate, but can be used under the modified silicone oil base, and the inherent disinfection effect of the rutaceae plant extract can be exerted only under the condition that bacteria and mildew do not grow or under the environment without nutrients available for microorganisms.

From the above examples, it is known that rutaceae plant extracts can achieve general antibacterial effects on water as a base, and can completely exert disinfection effects on modified silicone oil as a base, so that natural gels with disinfection effects are prepared, and not only is the antiseptic effect good, but also the proportion of the antiseptic and the polyhydric alcohol is adjusted to be synergistic, so that short-time antibacterial disinfection can be achieved.

Example 4: adding aqueous solution of extract of Rutaceae plant into water-based natural polyalcohol, natural thickener and natural antiseptic

In this example, the ingredients and the ratio of the aqueous solution of rutaceae plant extract to the water-based polyol, natural thickener and preservative are shown in table 4, and the results of the antibacterial activity test data are shown in table 5.

TABLE 4

TABLE 5

As shown in the data in Table 5, the formulation of example 4 has at least 99.9% of inhibition efficiency against four bacteria, namely Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans, within 20 minutes.

The anti-HIV-1 virus test of the formulation of example 4 is described below. FIG. 1 shows a simple 1.35 wt% aqueous solution of a Rutaceae plant extract (sample 1); polyol system, preservative and natural thickener (sample 2); and comparison of rutaceae plant extract aqueous solutions, polyol systems, preservatives and natural thickeners (sample 3) against HIV-1 virus test. As can be seen from fig. 1, sample 3 is more effective against bacteria, corrosion and viruses than either sample 1 or sample 2.

FIG. 2 is a graph of a single 1.35 wt% aqueous solution of a Rutaceae plant extract (sample 1) using an 80-fold dilution; polyol system, preservative and natural thickener (sample 2); and comparison of the ability of aqueous rutaceae plant extract solutions, polyol systems, preservatives and natural thickeners (sample 3) to inhibit HIV-1 infection of JLTRG CD4+ cells. As seen in FIG. 2, sample 3 was more potent in antibacterial, preservative and antiviral effects than either sample 1 or sample 2, and had achieved the effect of eighty percent of Azidothymidine (AZT), a positive control drug.

Table 6 shows the 50% inhibitory concentration against HIV-1 infection for sample 3 as a dilution of sample 3.

TABLE 6

Cell line	IC₅₀(dilution factor of sample 3)
		Magi-5	233
Jurkat(JLTRG)	312

The foregoing examples show that the antimicrobial efficacy of the aqueous extract of rutaceae plants, the polyol system, the preservative and the thickener is better than that of the pure aqueous extract of rutaceae plants or the composition of natural thickener added to the aqueous extract of rutaceae plants, and the antimicrobial efficacy can reach 99.9%. In addition, taking HIV-1 test as an example, the antiviral effect of the composition of rutaceae plant extract aqueous solution, polyol system, preservative and natural thickener (sample 3) was better than that of the composition of rutaceae plant extract aqueous solution alone (sample 1) or the composition of polyol system, preservative and natural thickener (sample 2), and was about 80% of the antiviral ability of the positive control drug AZT, and the experimental conditions were 80 times diluted samples. Therefore, it is estimated that sample 3 has a very significant antibacterial and antiviral efficacy of 99.9% without dilution.

Example 5: adding aqueous solution of extract of Rutaceae plant into water-based solution containing polyalcohol, natural thickener and antiseptic

Example 5 the ingredients and proportions of the aqueous solution of rutaceae plant extract added to a water-based composition containing a polyol, a natural thickener, and a preservative are shown in table 7. Example 5 differs from example 4 in that it uses different polyols and can achieve the same level of antimicrobial effect in different proportions.

TABLE 7

Numbering	Composition (I)	wt％
			1	Aqueous solution of extract of rutaceae plant	1.5
2	Aloe extract	0.1
			3	Hyaluronic acid powder	0.1
4	Sodium anisate and sodium levulinate	3.0
			5	Lactic acid	0.2
6	Sanxian glue	2
			7	Pentanediol	3.5
8	Pure water	The rest(s)

The composition containing the Rutaceae plant extract is suitable for various formulations, and is not suitable for being made into gel or cream products, unlike the situation that many disinfectants can only be made into aqueous solutions, or most cationic bactericides cannot be matched with most natural thickeners (most of which are anions), for example, products such as water spray, white factors, gold shields and the like which are sold in the market are in aqueous solution forms.

The above-described embodiments are merely exemplary for convenience of description, and various modifications may be made by those skilled in the art without departing from the scope of the invention as claimed in the claims.

Claims

1. A composition containing Rutaceae plant extract is characterized by comprising 0.1-2 wt% of Rutaceae plant extract, 10-30 wt% of C1-C4 polyalcohol system and 60-70 wt% of pure water.

2. A composition containing Rutaceae plant extract is characterized by comprising 0.1-2 wt% of Rutaceae plant extract, 5-15 wt% of C5-C8 polyalcohol system and 60-70 wt% of pure water.

3. A composition containing Rutaceae plant extract is characterized by comprising 0.1-2 wt% of Rutaceae plant extract; 10-30 wt% of a C1-C4 polyol system; 1-4 wt% preservative; 0.5-2 wt% of natural thickening agent and 60-70 wt% of pure water.

4. A composition containing Rutaceae plant extract is characterized by comprising 0.1-2 wt% of Rutaceae plant extract; 2-10 wt% of a C5-C8 polyol system; 1-4 wt% preservative; 1-3 wt% of natural thickening agent and 60-70 wt% of pure water.

5. The composition of any one of claims 1 to 4, wherein the extract of a Rutaceae plant is an extract selected from any one of the group consisting of oranges, mandarins, sweet oranges, lime, bitter oranges, pomelos, grapefruits, lemons, lime, lemons, bergamots, and kumquats.

6. The rutaceae plant extract-containing composition of claim 1 or 3, wherein the C1-C4 polyol system is a composition selected from any one of the group consisting of glycerol, propylene glycol, methyl propylene glycol, and butylene glycol.

7. The rutaceae plant extract-containing composition of claim 2 or 4, wherein the C5-C8 polyol system is a composition selected from any one of the group consisting of pentanediol, hexanediol, octanediol, and octanediol.

8. The composition of claim 3 or 4, wherein the natural thickener is a composition selected from the group consisting of xanthan gum, guar gum, sodium alginate and gum arabic.

9. The composition of claim 3 or 4, wherein the preservative is selected from the group consisting of sodium anisate and sodium levulinate; octanoyl hydroxamic acid and lactic acid; or glyceryl caprylate and glyceryl undecylenate.

10. The rutaceae plant extract-containing composition of any one of claims 1-4, further comprising hyaluronic acid powder and aloe vera extract.