CN110538244B - Traditional Chinese medicine composition for promoting skin wound healing - Google Patents
Traditional Chinese medicine composition for promoting skin wound healing Download PDFInfo
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- CN110538244B CN110538244B CN201910811373.XA CN201910811373A CN110538244B CN 110538244 B CN110538244 B CN 110538244B CN 201910811373 A CN201910811373 A CN 201910811373A CN 110538244 B CN110538244 B CN 110538244B
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Abstract
The invention discloses a traditional Chinese medicine composition for promoting skin wound healing, which is prepared from 3-7 parts of valerian volatile oil extract, 2-4 parts of borneol and 10-20 parts of egg yolk oil extract. The invention also provides a preparation method of the valerian volatile oil extract and the egg yolk oil extract. The traditional Chinese medicine composition has the effect of promoting the healing of skin wounds such as lacerations, scratches, scalds or anal fissures, skin ulcers or pustules and the like.
Description
Technical Field
The invention relates to a traditional Chinese medicine composition, in particular to a traditional Chinese medicine composition for promoting skin wound healing, and belongs to the field of traditional Chinese medicines.
Background
The skin is the largest organ of the human body, is one of the tissues with the fastest self-renewal speed of the body, and is the first defense line for preventing the body from dehydration, injury and infection. After the skin is damaged, a series of complex and coordinated processes appear in the body, and various cell components including inflammatory cells and tissue repair cells participate in the processes to promote the healing of the skin wound.
For a normal human body, light skin injury does not need special treatment, and wounds can recover within a certain time, but for some wounds which are difficult to heal, such as diabetes, nutritional deficiency, steroid diseases and even anal fissure wounds, or for post personnel which are easy to hurt and need quick wound healing, such as soldiers, policemen, first-line workers, farmers and the like, a callus which can be conveniently and effectively used is still needed.
However, in the prior art, there are not many researches on drugs for repairing damaged skin, and at present, the main drugs include Yunnan white drug powder (mainly used for traumatic injuries), Mayilong hemorrhoid ointment (used for anal fissure) and the like, but the effect is not satisfactory.
volvalerenal K (1-hydroxy-1,11,11-decahydrocyclopropane azulene-10-one) is a sesquiterpene with a molecular formula of C14H22O2(m/z245.151 1[M+Na]+，calcd for C14H22O2Na，245.151 7)。
The research on volvalerena K in the field is relatively few, and the inventor has extracted and isolated volvalerena K from valeriana latifolia for the first time (national institute of military medical science, doctor's academic paper "research on active ingredients of Valeriana latifolia against H5N1 virus", revised compound 33 is named as volvalena K), and the research finds that volvalena K can improve the learning and memory capacity of Alzheimer disease model mice by regulating related enzymes of cholinergic nervous system (China New medicine, 2016, volume 25, phase 13, APP improvement on learning and memory of birotogenetic dementia mice by volvalerena K in Valeriana latifolia (APP) through research and study.
The inventors continued research on volvalerena K.
Disclosure of Invention
The invention aims to provide an external traditional Chinese medicine composition which can be used for treating various skin damages.
The traditional Chinese medicine composition is prepared from the following raw material medicines in parts by weight: 3-7 parts of valerian volatile oil extract, 2-4 parts of borneol and 10-20 parts of egg oil extract. The preferred mixture ratio is 5 parts of valerian volatile oil extract, 3 parts of borneol and 15 parts of egg yolk oil extract. Wherein the content of volvalenal K in the valerian volatile oil extract is not less than 10%.
The preparation method of the traditional Chinese medicine composition comprises the following steps:
1) preparation of a Valeriana officinalis volatile oil extract
Taking dried rhizome of valeriana latifolia, fully soaking the dried rhizome in 10 times of 60% ethanol solution for 12h, heating and refluxing for extraction for 3 times, each time for 1.5h, combining the extracting solutions, concentrating under reduced pressure to obtain an extract, extracting with trichloromethane, and concentrating under reduced pressure to recover the solvent. The chloroform extract is subjected to gradient elution by silica gel column chromatography (petroleum ether: acetone 100:0, 100:1, 75:1, 50:1, 30:1, 25:1, 20:1, 15:1, 10:1, 7:1, 5:1, 3:1, 2:1, 1:1 and methanol), elution fractions are subjected to reduced pressure concentration, and similar fractions are combined by detection of a thin-layer plate to obtain 15 components with the numbers of A to O. And (3) performing silica gel column chromatography on the fraction I by adopting chloroform-methanol gradient elution (200: 1-1: 1), combining eluates in 10-35 bottles (the flow rate is 1mL/min, 5 mL/bottle, and collecting according to a thin layer chromatography detection result), and volatilizing the solvent to obtain a yellow oily compound, namely the valerian volatile oil.
Taking three parts of the yellow oily compound, each 3.00g of the yellow oily compound, respectively carrying out gradient elution by silica gel column chromatography chloroform-methanol (30:1) and the like, automatically collecting by an automatic collector, combining 25 th-28 th tube eluent (the flow rate is 1mL/min, 5 mL/tube, and collecting according to a thin-layer chromatography detection result), concentrating by gel column chromatography (the chloroform-methanol is 1:1), combining 16 th-21 th bottle eluent (the flow rate is 1mL/min, 5 mL/bottle, and collecting according to a thin-layer chromatography detection result), volatilizing the solvent to obtain white crystals, namely the volvalenal K, wherein the purity of the volvalenal K is respectively 30mg, 32mg and 35mg, and the purity of the volvalenal K is more than 98% through mass spectrometry and nuclear magnetic resonance spectroscopy, so that the content of the volvalenal K in the volatile oil is determined to be about 10% -12%.
2) Preparation method of egg yolk oil extract
Boiling egg, mixing yolk with sesame oil at a weight ratio of 30:1, heating with slow fire in a pot, stirring, removing water, frying with strong fire until yolk turns from yellow to black, making sound and oil overflow, and collecting brown yolk oil. Filtering with 240 mesh fine gauze, filtering with Sephadex LH-20 gel column chromatography, gradient eluting with chloroform-methanol (1:1), collecting 10-30 tubes of eluate (flow rate of 1mL/min and 5 mL/tube, collecting according to thin layer chromatography detection result), mixing, and volatilizing solvent to obtain egg yolk oil extract.
3) Mixing the above rhizoma et radix Valerianae volatile oil extract, egg yolk oil extract and Borneolum Syntheticum at a certain proportion. The composition can be used directly or made into topical preparation such as ointment, cream, powder, etc.
The traditional Chinese medicine composition combines the traditional Chinese medicine theory and clinical pharmacology, combines the research results of pharmacy and pharmacology, has the effects of relieving swelling and pain, stopping bleeding, promoting granulation and promoting healing, and is a good medicine for treating skin cracks and trauma. Can be used for treating skin surface laceration, bleeding symptoms, including laceration due to puncture, scratch, scald, anal fissure, etc., especially intractable skin laceration due to various reasons, and the laceration position includes, but is not limited to, the above skin.
Description of the drawings:
FIG. 1: structure of volvalerenal K molecule
FIG. 2: preparation flow chart of valerian volatile oil extract containing volvalenal K
FIG. 3: process for preparing egg yolk oil extract
Detailed Description
The first embodiment is as follows: clinical effect verification
The technical scheme of the invention is started in 2011, and proved to be practical and effective in healing the skin cracks by trial of 10 ten thousand patients, the treatment effects of anal fissure, scratch and scald are respectively considered, and the results are as follows:
1) can be used for treating anorectal diseases such as anal fissure
Selecting 1000 patients diagnosed as anal fissure by a hospital during 10 months from 2011 to 2015 as study objects, and randomly dividing the study objects into a control group and a treatment group, wherein the control group comprises 243 men and 257 women, the age is 1-80 years, and the average age is 40.26 years; 256 men and 244 women with age of 1-85 years and average age of 41.36 years in the treatment group, and the average age and general data of the patients in the two groups are compared without significant difference (P > 0.05).
Inclusion criteria were: patients with various anal fissures; the wound is not healed for more than 1 week; the patients gave informed consent.
Experimental drugs: mayilong musk hemorrhoid ointment is purchased from Mayilong pharmaceutical group GmbH.
The liquid medicine is prepared by mixing 5 parts of valerian volatile oil extract, 3 parts of borneol and 15 parts of egg yolk oil extract.
The experimental method comprises the following steps: the control group adopts the Mayinglong musk hemorrhoid ointment for comparison, patients in the treatment group clean the anus with clear water after each defecation, wipe the anus dry, dip the liquid medicine with a cotton swab and evenly spread the liquid medicine inside and outside the anus, wipe the anus dry after half an hour, 5 days are 1 treatment course, and clinical efficacy is observed after 10 days. Patients who were not treated by the control group after 10 days were treated by the recipe, and clinical effects were observed after 5 days.
Wherein, the wound is cured after being completely healed, the wound becomes effective after being reduced, and the wound is ineffective without obvious change.
TABLE 1 therapeutic effect comparison table
TABLE 2 therapeutic effect of the present recipe instead of the control therapy
Through the clinical observation of 1000 patients in 5 years, the clinical test shows that the medicine has obviously better curative effect on anal fissure than Mayilong, and has the advantages of rapid healing and short time, and 66.54 percent of patients can be cured in 3 days in all cured patients. Moreover, the patient age span selected by the recipe is large, and the recipe is suitable for patients of all ages.
2) Therapeutic effect on surface skin wound such as scratch
100 patients scratched by a sharp instrument during 6 months from 2013 to 2014 were selected as study subjects, and were randomly divided into a control group and a treatment group, wherein 50 patients have no significant difference (P & gt 0.05) when the average age and the general data of the two patients are compared.
Inclusion criteria were: the length of the wound is within the range of 0.5-3 cm, and the width is within the range of 0.2 mm; slight or no bleeding from the wound; the patients gave informed consent.
Experimental drugs: yunnan white drug powder, commercially available, produced by Yunnan white drug powder group member company;
the liquid medicine is prepared by mixing 5 parts of valerian volatile oil extract, 3 parts of borneol and 15 parts of egg yolk oil extract.
The experimental method comprises the following steps: the control group is treated by Yunnan white drug powder, the patients in the treatment group are dipped in the liquid medicine by cotton swabs and evenly smeared, and the healing effect is observed after 5 days.
Wherein the wound is completely healed to be cured, the wound becomes effective when the wound becomes small, and the wound is ineffective without obvious change.
TABLE 3 comparison table of healing effect of wound
Clinical experiment results show that the traditional Chinese medicine can obviously promote the healing of skin wounds, and the effective rate is 100%.
3) Has therapeutic effect on scald
50 patients with mild burns and scalds in 10 months from 2014 to 2016, 32 men and 18 women are selected; age 10-72 years, mean (40.7 ± 20.2) years; 22 superficial first degree burns and 28 second degree burns have wound area of 7% at most and 1% at least.
Inclusion criteria were: superficial burn and scald; the area of burn and scald is less than 10%; within 12h of injury; the scald parts are mostly positioned on the upper limbs, the upper abdomen and the middle lower part of the lower leg 1/3; shock symptoms are not evident.
The treatment method comprises the following steps: the liquid medicine is dipped by a cotton swab and evenly smeared on the wound surface of the burn and scald, and the treatment effect is observed after 7 days.
And (3) curing: the new epithelium on the wound surface is covered completely, and the wound surface is dry and clean without exudation, pain and infection. Improvement: the wound surface is covered with new epithelium, and is dry and tidy, red and swollen, slightly painful, and free of exudation and infection. And (4) invalidation: the wound surface is red and swollen, and has more effusion and infection.
TABLE 4 therapeutic effects on burn and scald
Clinical results for 7 days showed that 50 cases were effective. Pain was relieved within 5 min. Wherein 2 cases are cured in 3 days, 6 cases are cured in 4 days, 8 cases are cured in 5 days, 10 cases are cured in 6 days, 4 cases are cured in 7 days, and the rest are all obviously improved.
Example two: parallel comparative screening experiment
According to literature and pharmacological analysis, the valerian volatile oil extract, the borneol and the egg yolk oil extract are screened from more than ten possible raw material medicaments, and the matching modes of the three raw materials are determined by parallel comparison screening experiments according to the treatment effects of different combination modes on anorectal diseases such as anal fissure and the like.
1) The medicine is basically set as follows:
valerian volatile oil extract (A), borneol (B), egg yolk oil extract (C), valerian volatile oil extract (A) + borneol (B), valerian volatile oil extract (A) + egg yolk oil extract (C), borneol (B) + egg yolk oil extract (C), valerian volatile oil extract (A) + borneol (B) + egg yolk oil extract (C), and valerian volatile oil extract (A) + borneol (B) + egg yolk oil extract (C) in 7 groups.
700 patients diagnosed as anal fissure through a hospital during the period of 2011-2015 10 months are selected as study objects and randomly divided into a control group and a treatment group, wherein 100 patients have no significant difference (P > 0.05) when the average age and the general data of the patients are compared.
Inclusion criteria were: patients with various anal fissures; the wound is not healed for more than 1 week; the patients gave informed consent.
The experimental method comprises the following steps: after each group of patients defecate, the anus is cleaned by clear water, and after being wiped dry, the group of medicines are dipped in cotton swabs and evenly smeared inside and outside the anus, 5 days are 1 course of treatment, and the clinical curative effect is observed after 10 days.
Wherein, the wound is cured after being completely healed, the wound becomes effective after being reduced, and the wound is ineffective without obvious change.
TABLE 1 therapeutic effect comparison table
Through parallel clinical experiment analysis, the single effect of the three medicines is not ideal, A is superior to C, C is superior to B, the effective rate of A is 50.00 percent, and B is only 41.03 percent. After the three medicines are combined in pairs, the medicine effect is improved, wherein the effective rate of the combination of A and C is obviously improved to 64.00 percent, but the difference of the effective rate and the compatibility effect (98.00 percent) of the three medicines is larger, so the effect of the combination of the three medicines for promoting the healing of the skin cracks is optimal, and the clinical treatment effect of any one or two medicines is obviously better. In the treatment, the individual allergic phenomena of the extracts of borneol and egg yolk oil are found, and no allergic people appear in the compatible use process of the three medicines.
Claims (4)
1. A traditional Chinese medicine composition for promoting skin wound healing is characterized by being prepared from the following raw material medicines in parts by weight: 3-7 parts of valerian volatile oil extract, 2-4 parts of borneol and 10-20 parts of egg oil extract, wherein the content of volvalenal K in the valerian volatile oil extract is not less than 10%.
2. The traditional Chinese medicine composition according to claim 1, which is characterized in that the mixture ratio of the raw materials is as follows: 5 parts of valerian volatile oil extract, 3 parts of borneol and 15 parts of egg yolk oil extract.
3. Use of a Chinese medicinal composition according to claim 1 or 2 in the manufacture of a medicament for promoting healing of a skin wound.
4. Use according to claim 3, characterized in that said skin wound comprises a laceration due to a puncture, a scratch, a scald or an anal fissure, a skin ulcer or an abscess.
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| CN1319430A (en) * | 2001-03-05 | 2001-10-31 | 付长玉 | Pikang powder medicine for curing dermatosis |
| CN101019956A (en) * | 2007-03-19 | 2007-08-22 | 殷彬 | Bedsore treating medicine |
| CN102755271B (en) * | 2012-07-19 | 2014-04-09 | 贵州宏宇药业有限公司 | Compound essential oil with pressure relieving function and preparation method thereof |
| CN104510929A (en) * | 2013-10-02 | 2015-04-15 | 张勤明 | Traditional Chinese medicine composition for treating haemorrhoids |
| CN103877247B (en) * | 2014-04-03 | 2015-08-12 | 张良洁 | Medicine being used for the treatment of decubital ulcer and preparation method thereof |
| CN104127813A (en) * | 2014-07-24 | 2014-11-05 | 朱秀芳 | Traditional Chinese medicinal composition for curing qi-blood deficiency type bedsore and preparation method thereof |
| CN107648287B (en) * | 2017-10-16 | 2020-08-11 | 浙江中医药大学 | Young chicken paste ointment and application thereof in preparation of medicine for treating cracked nipple of lying-in woman |
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