CN110537997A - Special device for tumor transplantation of zebra fish juvenile fish and working method - Google Patents
Special device for tumor transplantation of zebra fish juvenile fish and working method Download PDFInfo
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- CN110537997A CN110537997A CN201810533286.8A CN201810533286A CN110537997A CN 110537997 A CN110537997 A CN 110537997A CN 201810533286 A CN201810533286 A CN 201810533286A CN 110537997 A CN110537997 A CN 110537997A
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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Abstract
The invention relates to a special device for tumor transplantation of zebra fish juvenile fish and a working method thereof. Four cuboid stand columns are respectively arranged at four corners of the lower bottom surface of the transplant body, and a plurality of grooves are arranged on the inner bottom surface. The inner bottom surface of each groove is provided with a plurality of drain holes and a scale, and the left inner side wall and the right inner side wall are provided with a plurality of groups of scale marks. The device is beneficial to fixing the position of the juvenile fish during tumor transplantation and is convenient to operate; but also can accurately quantify the dose of the transplanted tumor cells. In addition, the device can also effectively avoid the damage of juvenile fish caused by mistaken suction of juvenile fish in the process of sucking redundant liquid before tumor transplantation; but also is beneficial to saving the transplanting time and avoiding the death of the juvenile fish caused by water shortage due to overlong operation time. The culture device is ingenious in design concept, convenient to use, obvious in effect and good in effect, the experimental accuracy is guaranteed, and the working efficiency is improved.
Description
Technical Field
The invention relates to a special device for tumor transplantation of zebra fish juvenile fish and a working method, and belongs to the technical field of experimental animal research devices.
Background
Model biological tumor transplantation is an important approach in tumor biological research. Tumor transplantation is particularly important for analyzing and researching cell malignancy and migration capacity, and is the key for identifying potential tumor stem cells. In addition, model biological tumor transplantation plays an important role in drug research, and can be used for screening drugs with tumor inhibition activity or optimizing tumor treatment schemes.
Zebrafish (zebrafish, Danio rerio) is one of the three common model organisms in the field of life sciences. It has the characteristics of high egg laying amount, low feeding cost, short breeding period, in-vitro fertilization, in-vitro development, transparent embryo and the like; but also has similar physiological and biochemical characteristics of mammals. Compared with traditional model organisms such as mice and the like, zebrafish has unique advantages in tumor transplantation: 1) good tumor formation: zebrafish are not adaptive within about 4 to 6 weeks of fertilization. Therefore, the tumor formation of human tumor cells after xenotransplantation is good, and the existing data show that about 80 percent of human tumor cells can survive after being transplanted into the zebra fish body; 2) the indexes are visual and various: the whole body of the zebra fish juvenile fish is transparent and easy to observe; besides the evaluation of the size, survival rate and survival time of tumor tissues, the method can also observe the abnormal proliferation of blood vessels induced by tumor cells, the relationship between tumor metastasis and blood vessel proliferation and the like; 3) the experimental period is short: the pharmacodynamic evaluation can be completed within 3 days after administration. A large number of verification experiment results show that the prediction of the anti-cancer pharmacodynamics evaluation experiment result obtained by using zebra fish experimental administration for 3 days is very good; 4) the sample dosage is small: the early sample preparation is very difficult, and the zebra fish model can complete the experiment only by about 10mg of samples, so that the time and the cost required by sample preparation are saved; 5) the biological properties are conserved: a large number of documents show that the biological properties of human tumor cells are basically stable after the human tumor cells are transplanted into zebra fish bodies.
When the zebra fish is transplanted with the tumor, the young fish is usually placed in a culture dish, and then the dosage of the transplanted tumor is controlled by adjusting the injection air pressure and the injection time in combination with the microinjection technology. However, since the length and thickness of the injection needle greatly affect the injection volume, if the injection needle is replaced under the condition that the injection pressure and the injection time are the same, the injection volume is likely to be changed, and the dose of the transplanted tumor is also changed correspondingly. When the injection pressure and time are fixed, the volume of the injection in the juvenile fish body is observed by means of the indicator only through naked eyes, so that the accurate quantification of the injection volume cannot be realized, and the actual dose of the transplanted tumor cannot be accurately calculated.
Aiming at the defects of the existing culture dish for tumor transplantation, a special device for the tumor transplantation of the zebra fish juvenile fish needs to be designed urgently to solve the problem that the exogenous tumor transplantation dosage is inaccurate in the existing transplantation process.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a special device for tumor transplantation of zebra fish juvenile fish.
The invention also provides a working method of the device.
The technical scheme of the invention is as follows:
A special device for tumor transplantation of zebra fish juvenile fish comprises a box body and a transplant body;
the box body and the transplant body are rectangular concave bodies with baffles arranged on the periphery, the transplant body is positioned in the concave body of the box body, at least two rectangular grooves are arranged on the inner bottom surface of the transplant body, at least two circular drain holes and 1 scale are arranged at the bottom of each rectangular groove, and at least two groups of scale marks are arranged on the inner longitudinal wall of each rectangular groove;
Four cuboid stand columns are arranged at four corners of the outer bottom surface of the transplant body.
The transplant is inserted into the box body and then combined into a whole. The design has the advantages that the rectangular box body and the transplant body are easy to process and manufacture, and meanwhile, the internal space of the rectangular transplant body is large, so that a plurality of grooves are convenient to arrange; the rectangular groove in the transplant body is similar to the zebra fish juvenile fish in body type, so that the fish body can be conveniently fixed in the tumor transplantation process; when the tumor is transplanted, the transfer pipette is used for placing the juvenile fish into the groove of the transplant body, and redundant liquid in the transfer pipette can enter the box body through the drain hole at the bottom of the groove, so that the transplanting operation is prevented from being interfered, and meanwhile, the bottom of the box body is kept dry and can directly enter the next observation procedure. In addition, when the liquid in the box body is too much, in order to avoid liquid to spill into the transplant again, can take out the transplant from the box body to pour out the liquid in the box body, the transplant that takes out accessible four cuboid stands are fixed in on the operation panel, and can keep its bottom surface unable contact operation panel down, thereby avoid polluting.
Preferably, the baffle plates on two opposite sides of the implant body are obliquely arranged. The advantage of this design lies in, sets up both sides baffle into the slope, makes things convenient for the transplant body to get in the box body and puts.
Preferably, horizontal handles are arranged on two opposite sides of the transplant body and connected with the upper edge of the baffle of the transplant body. The advantage of this design lies in, the relative horizontal handle that sets up in transplant body left and right sides, is convenient for take out and put the transplant body from the box body.
Further preferably, two circular arc-shaped grooves are formed in the upper surfaces of the horizontal handles on the two opposite sides of the transplant body. The design has the advantages that the horizontal handles on the left side and the right side of the transplant body are respectively provided with two arc-shaped grooves, so that the needle holder can be fixed temporarily in the tumor transplantation process.
Preferably, the scale is arranged in the middle of the rectangular groove. The advantage of this design lies in, when a plurality of zebra fish juvenile fishes carry out tumor transplantation, arrange zebra fish juvenile fishes in proper order in both sides about the scale, can prevent effectively that tumor transplantation in-process juvenile fish position from taking place to remove, convenient transplantation. And the diameter of a spherical liquid drop formed after the culture solution containing the tumor cells enters the juvenile fish can be measured by using a ruler, and the accurate dose of the tumor cells transplanted each time can be calculated.
Preferably, the number of the scale marks is 4-10 groups, and the distance between every two groups of the scale marks is 2-7 mm.
Further preferably, the number of the graduation lines is 6 groups, and the distance between each group of graduation lines is 4 mm.
Further preferably, each group of scale marks comprises 4 scales, and the corresponding scales are 1mm, 2mm, 3mm and 4mm in sequence. The design has the advantages that when the zebra fish juvenile fish is transplanted with the tumor, the diameter of the spherical liquid drop formed after the culture solution containing the tumor cells enters the juvenile fish can be measured by using the scale marks in an auxiliary mode, and then the dosage of the tumor cells transplanted each time can be accurately calculated.
Preferably, the cuboid stand is articulated with the external bottom surface of transplantation, and four cuboid stands link to each other through three connecting rod, and one of them cuboid stand still articulates and is equipped with the elbow peg. The cuboid stand column can be rotationally parallel to the outer bottom surface of the transplant body, so that the space between the transplant body and the box body can be further shortened, and the condition that the whole device is too high to influence the follow-up microscopic observation is avoided. When the device needs to be erected, the elbow hanging rods are pulled, the four stand columns are enabled to be in an upright state under the action of the connecting rods, then the elbow hanging rods are hung on the edge of a transplant body, and when the device needs to be folded, the elbow hanging rods are taken down to enable the cuboid stand columns to rotate to be horizontal and can be folded.
Preferably, the bottom of the rectangular groove is provided with a filter screen, and a scale is arranged above the filter screen. And a filter screen is used for replacing a circular drain hole to filter off excessive water.
Preferably, the number of the rectangular grooves is 3-6.
Further preferably, the number of the rectangular grooves is 5.
Preferably, the number of the circular drain holes is 20-60.
Further preferably, the number of the circular drain holes is 40.
Preferably, the transparent box body is 70-110mm long; the width is 50-70 mm; the thickness is 1.5-2.5 mm.
Further preferably, the box body is 90mm long; the width is 60 mm; the thickness is 2 mm.
Preferably, the graft is 50-100mm long; the width is 45-60 mm; the thickness is 2-6 mm.
Further preferably, the graft is 80mm long; the width is 55 mm; the thickness is 5 mm.
Preferably, the baffle of the box body is 10-30mm high and 1.5-2.5mm thick.
Further preferably, the baffle of the box body is 25mm high and 2mm thick.
Preferably, the barrier of the graft is 10-30mm high and 0.5-2.5mm thick. .
more preferably, the barrier of the graft is 20mm high and 1mm thick.
Preferably, the horizontal handle is 45-60mm long and 5-20mm wide; the thickness is 5-13 mm.
Further preferably, the horizontal handle is 55mm long; the width is 15 mm; the thickness is 9 mm.
Preferably, the arc-shaped groove is 45-60mm long, 4-8mm deep and 6-15 mm wide-mouth length.
More preferably, the arc-shaped groove is 55mm long, 7mm deep and 11mm wide.
Preferably, the cuboid stand column is 1-4mm long, 1-4mm wide and 2-5mm high.
Further preferably, the cuboid stand column is 3mm long, 2mm wide and 4mm high.
preferably, the rectangular groove is 40-50mm long; the width is 5-15 mm; the depth is 1.5-4.7 mm; the distance between adjacent rectangular grooves is 3-7 mm.
Further preferably, the rectangular groove is 45mm long; the width is 10 mm; depth 4.5 mm; the distance between adjacent rectangular grooves is 5 mm.
Preferably, the scale is 40-50mm long; 1-3mm wide; the thickness is 0.1-0.7 mm.
further preferably, the ruler is 45mm long; the width is 2 mm; the thickness is 0.3 mm.
Preferably, the diameter of the circular drainage hole is 0.5-2 mm.
Further preferably, the diameter of the circular drainage hole is 1 mm.
Preferably, the minimum unit of the scale markings is 0.05-3 mm.
Further preferably, the minimum unit of the scale markings is 0.1 mm.
A working method of the special device for tumor transplantation of the zebra fish juvenile fish comprises the following steps:
Inserting the transplant into the box body, transferring the zebra fish juvenile fish into the groove of the transplant by using a pipette, moving the transplant out of the box body, pouring out the liquid in the box body, inserting the transplant into the box body again, performing tumor transplantation by using a needle holder, and measuring the diameter of a spherical liquid drop formed after a culture solution containing tumor cells enters the juvenile fish.
The invention has the beneficial effects that:
The invention provides a special device for tumor transplantation of zebra fish juvenile fish, which is provided with a transplant body, wherein a plurality of rectangular grooves are arranged in the transplant body, and the inner bottom surface and the inner side wall of each rectangular groove are respectively provided with a scale and a scale mark. When the tumor is transplanted, the juvenile fishes are placed on two sides of the scale in the rectangular groove, so that the positions of the juvenile fishes can be adjusted conveniently and arranged neatly, the juvenile fishes are prevented from moving in the transplanting process, and the tumor transplantation is facilitated; the diameter of the spherical liquid drop formed after the culture solution containing the tumor cells enters the juvenile fish can be measured by the ruler and the scale lines, and the accurate dose of the tumor cells transplanted each time can be calculated according to the sphere volume V ═ pi d 3/6.
According to the technical scheme, horizontal handles are arranged on the left side and the right side of the transplant body, and arc-shaped grooves are formed in the horizontal handles. In the experimental process, if the tumor transplantation is temporarily stopped, the needle holder can be temporarily fixed in the arc-shaped groove, so that the needle holder is prevented from rolling, and the injection needle is prevented from being broken due to the rolling of the needle holder.
Four rectangular upright posts are respectively arranged at four corners of the lower bottom surface of the transplant body, a plurality of rectangular grooves are arranged on the inner bottom surface, and circular drain holes are formed in the rectangular grooves. When the tumor is transplanted, the transplant bodies are inserted into the box body and then combined into a whole; the transfer pipette is used for placing the juvenile fish into the groove of the transplant body, and the excess water in the groove can be quickly drained into the box body through the drain hole at the bottom of the groove, so that the juvenile fish can be prevented from being sucked by mistake when the water is sucked, and the injury to the juvenile fish is avoided; the experimental time can be saved, the death of the juvenile fish due to long-time water shortage can be avoided, and the microscope can be prevented from being polluted by residual liquid drops due to the drying of the lower surface of the box body, so that the experimental operation is convenient. In addition, when the moisture in the box body is excessive, the transplant body can be taken out of the box body, and the moisture in the box body is poured out, so that the situation that the moisture overflows into the transplant body again to influence the experimental operation is avoided. The transplant body that takes out can be fixed in on the operation panel through four cuboid stands, and its lower bottom surface can't contact the operation panel to avoid polluting. Convenient to use, the effect is obvious, and is respond well, has guaranteed the experiment accuracy, has improved work efficiency.
Drawings
Fig. 1 is a schematic structural diagram of a cartridge body according to the present invention.
FIG. 2 is a schematic view showing the structure of the graft according to the present invention.
Fig. 3 is a top view of the cartridge of the present invention.
Fig. 4 is a top view of the implant of the present invention.
FIG. 5 is a schematic view of the assembly of the cassette and the graft according to the present invention.
FIG. 6 is a schematic view of the structure of the graft and the rectangular parallelepiped pillar in the present invention.
Wherein: 1. the box body 101, the box body baffle plate 102, the box body baffle plate 2, the transplant body 201, the transplant body baffle plate 202, the transplant body baffle plate 3, the cuboid upright post 4, the rectangular groove 401, the drain hole 402, the scale 403, the scale mark 5, the horizontal handle 501 and the circular arc groove.
Detailed Description
the invention is further illustrated, but not limited, by the following examples in conjunction with the accompanying drawings.
Example 1
A special device for tumor transplantation of zebra fish juvenile fish is shown in figures 1-5 and comprises a transparent box body 1 and a transplant body 2, wherein the box body 1 and the transplant body 2 are combined into a whole. The box body 1 is made of transparent glass or plastic sheets; the box body 1 is rectangular in shape. The four side surfaces of the rectangular box body 1 are provided with baffle plates 101 and 102, and the baffle plates 101 and 102 are made of glass or plastic. The whole box body 1 is enclosed into a rectangular concave body by the surrounding baffle plates. The transplant 2 is made of transparent glass or plastic sheets; the shape of the transplant body 2 is rectangular, baffles 201 and 202 are arranged on four side surfaces of the rectangular transplant body 2, and the baffles 201 and 202 are made of glass or plastic. The whole transplant body 2 is surrounded by the baffle plates around to form a rectangular concave body.
Five rectangular grooves 4 are arranged on the inner bottom surface of the transplant body, 40 circular drain holes 401 and 1 scale 402 are arranged at the bottom of each rectangular groove, the scale is arranged in the middle of each rectangular groove, and 6 groups of scale marks 403 are arranged on the inner longitudinal wall of each rectangular groove.
Four cuboid columns 3 are arranged at four corners of the outer bottom surface of the transplant body. Wherein, the baffles 202 on the left and right sides of the transplant body 2 are obliquely arranged, and the horizontal handles 5 are arranged on the opposite positions of the left and right sides of the transplant body 2. The arrangement of the inclined baffle 202 and the horizontal handle 5 is convenient for the transplant body 2 to be taken and placed from the box body 1. Two circular arc grooves 501 are respectively arranged on the upper surfaces of the left and right horizontal handles 5. The arc-shaped groove 501 is convenient for temporary fixation of the needle holder in tumor transplantation operation.
When tumor transplantation is carried out, the transplant body 2 is inserted into the box body 1, the zebra fish juvenile fish is transferred into the groove 4 of the transplant body 2 by using a pipette, and redundant moisture in the groove 4 can be quickly drained into the box body 1 through the drain hole 401 at the bottom of the groove 4, so that the juvenile fish can be prevented from being sucked by mistake when the moisture is sucked, and the injury to the juvenile fish is avoided; the operation time of tumor transplantation is saved, the death of the zebra fish juvenile fish due to water shortage caused by overlong operation time is avoided, and the pollution of residual liquid drops to a microscope can be avoided due to the drying of the lower surface of the box body 1, so that the experiment operation is convenient. When the moisture in the box body 1 is excessive, the transplant body 2 can be taken out of the box body 1, and the moisture in the box body 1 is poured out, so that the situation that the moisture overflows into the transplant body again to influence the experimental operation is avoided. The transplant body 2 that takes out can be fixed in on the operation panel through four cuboid stands 3, and its lower bottom surface can't contact the operation panel to avoid polluting.
When tumor transplantation is carried out, young zebra fish are placed on two sides of the scale 402 in the groove 4, so that the position of the young zebra fish can be prevented from moving in the transplantation process, and the transplantation operation is convenient; the diameter of the spherical liquid drop formed after the culture solution containing the tumor cells enters the juvenile fish can be measured through the ruler 402 and the scale mark 403 in the groove 4, and then the accurate dose of the tumor cells transplanted each time can be calculated.
Example 2
A special device for tumor transplantation of zebra fish juvenile fish, which has the structure as described in embodiment 1, except that the device comprises a transparent box body and a transplant body, and the box body and the transplant body are combined into a whole. The box body is 70mm long, 50mm wide and 1.5mm thick, and the baffles all around are 10mm high and 1.5mm thick. The length of the transplant is 50mm, the width is 45mm, the thickness is 2mm, the height of the baffle plates at the periphery is 10mm, and the thickness is 0.5 mm. The horizontal handles on the two sides are 45mm long, 5mm wide and 5mm thick. The horizontal handle is provided with a circular arc groove with the length of 45mm, the depth of 4mm and the wide-mouth length of 6 mm.
Four corners of the lower bottom surface of the transplant body are respectively provided with 4 cuboid stand columns with the length of 2mm, the width of 1mm and the height of 2 mm; the inner bottom surface is provided with 4 rectangular grooves with the interval of 7mm, the length of 40mm, the width of 5mm and the depth of 1.5 mm. The inner bottom surface of each rectangular groove is provided with 1 scale and 30 circular drain holes with the diameter of 2 mm; and 4 groups of scale marks with the interval of 7mm are arranged on the left inner side wall and the right inner side wall, and each group of scale marks is totally 4 and respectively corresponds to 1mm, 2mm, 3mm and 4 mm. The ruler in the rectangular groove is 40mm long, 1mm wide, 0.2mm thick and 0.05mm minimum unit.
Example 3
The special device for tumor transplantation of the zebra fish juvenile fish is structurally as described in embodiment 1, except that the box body is 110mm long, 70mm wide and 2.5mm thick, and the peripheral baffles are 30mm high and 2.5mm thick. The length of the transplant is 100mm, the width is 60mm, the thickness is 6mm, the height of the baffle plates at the periphery is 30mm, and the thickness is 2.5 mm. The horizontal handles on two sides are 60mm long, 20mm wide and 13mm thick. The horizontal handle is provided with an arc-shaped groove with the length of 60mm, the depth of 8mm and the wide-mouth length of 15 mm.
The position of the four corners of the lower bottom surface of the transplant body is respectively provided with 4 cuboid stand columns with the length of 4mm, the width of 3mm and the height of 5mm, and the inner bottom surface is provided with 6 rectangular grooves with the interval of 3mm, the length of 50mm, the width of 15mm and the depth of 4.7 mm. The inner bottom surface of each rectangular groove is provided with 1 scale and 60 circular drain holes with the diameter of 0.5 mm; and 8 groups of scale marks with the interval of 2mm are arranged on the left inner side wall and the right inner side wall, and each group of scale marks is totally 4 and respectively corresponds to 1mm, 2mm, 3mm and 4 mm. The ruler in the rectangular groove is 50mm long, 3mm wide, 0.7mm thick and 3mm minimum unit.
Example 4
The special device for tumor transplantation of the zebra fish juvenile fish is structurally as described in embodiment 1, except that the box body is 90mm long, 60mm wide and 2mm thick, and the peripheral baffles are 25mm high and 2mm thick. The length of the transplant is 80mm, the width is 55mm, the thickness is 5mm, the height of the baffle plates at the periphery is 20mm, and the thickness is 1 mm. The horizontal handles on the two sides are 55mm long, 15mm wide and 9mm thick. The horizontal handle is provided with an arc-shaped groove with the length of 55mm, the depth of 7mm and the wide-mouth length of 11 mm.
The position of the four corners of the lower bottom surface of the transplant body is respectively provided with 4 cuboid stand columns with the length of 3mm, the width of 2mm and the height of 4mm, and the inner bottom surface is provided with 5 rectangular grooves with the interval of 5mm, the length of 45mm, the width of 10mm and the depth of 4.5 mm. The inner bottom surface of each rectangular groove is provided with 1 scale and 40 circular drain holes with the diameter of 1 mm; set up 6 groups of scale marks of interval 4mm on the inside wall about then, every group scale mark totally 4, correspond 1mm, 2mm, 3mm and 4mm respectively. The length of the scale in the rectangular groove is 45mm, the width is 2mm, the thickness is 0.3mm, and the minimum unit is 0.1 mm.
Example 5
The utility model provides a isolated plant for zebra fish juvenile fish tumor is transplanted, its structure is as embodiment 1 the difference is, the cuboid stand is articulated with the external bottom surface of transplantation, and four cuboid stands link to each other through three connecting rod, and one of them cuboid stand still articulates and is equipped with the elbow peg, as shown in figure 6. The cuboid stand column can be rotationally parallel to the outer bottom surface of the transplant body, so that the space between the transplant body and the box body can be further shortened, and the condition that the whole device is too high to influence the follow-up microscopic observation is avoided. When the device needs to be erected, the elbow hanging rods are pulled, the four stand columns are enabled to be in an upright state under the action of the connecting rods, then the elbow hanging rods are hung on the edge of a transplant body, and when the device needs to be folded, the elbow hanging rods are taken down to enable the cuboid stand columns to rotate to be horizontal and can be folded.
Example 6
The utility model provides a isolated plant for zebra fish juvenile fish tumour is transplanted, its structure as embodiment 1 the difference is, the bottom of rectangle recess is equipped with the filter screen, and the filter screen level top is equipped with the scale. And a filter screen is used for replacing a circular drain hole to filter off excessive water.
Claims (10)
1. A special device for tumor transplantation of zebra fish juvenile fish is characterized by comprising a box body and a transplant body;
The box body and the transplant body are rectangular concave bodies with baffles arranged on the periphery, the transplant body is positioned in the concave body of the box body, at least two rectangular grooves are arranged on the inner bottom surface of the transplant body, at least two circular drain holes and 1 scale are arranged at the bottom of each rectangular groove, and at least two groups of scale marks are arranged on the inner longitudinal wall of each rectangular groove;
Four cuboid stand columns are arranged at four corners of the outer bottom surface of the transplant body.
2. the special device for tumor transplantation of zebra fish juvenile fish according to claim 1, wherein the baffles on two opposite sides of the transplant body are inclined.
3. The special device for tumor transplantation of zebra fish juvenile fish according to claim 1, wherein horizontal handles are arranged on two opposite sides of the transplant body, and the horizontal handles are connected with the upper edge of the baffle plate of the transplant body.
4. The special device for tumor transplantation of the zebra fish juvenile fish of claim 3, wherein two circular arc-shaped grooves are formed on the upper surfaces of the horizontal handles at two opposite sides of the transplant body.
5. The special device for tumor transplantation of zebra fish juvenile fish according to claim 1, wherein the scale is arranged in the middle of the rectangular groove.
6. the special device for tumor transplantation of the zebra fish juvenile fish of claim 1, wherein the number of the graduation lines is 4-10 groups, and the distance between each group of graduation lines is 2-7 mm; each group of scale marks comprises 4 scales, and the corresponding scales are 1mm, 2mm, 3mm and 4mm in sequence.
7. The special device for tumor transplantation of zebra fish juvenile fish of claim 6, wherein the number of the graduation lines is 6 groups, and the distance between each group of graduation lines is 4 mm.
8. The special device for tumor transplantation of the zebra fish juvenile fish according to claim 1, wherein the rectangular column is hinged to the outer bottom surface of the transplantation body, four rectangular columns are connected through three connecting rods, and one of the rectangular columns is further hinged to an elbow hanging rod.
9. The special device for tumor transplantation of the zebra fish juvenile fish according to claim 4, wherein the number of the rectangular grooves is 3-6, the number of the circular drainage holes is 20-60, and the length of the box body is 70-110 mm; the width is 50-70 mm; the thickness is 1.5-2.5mm, and the length of the transplant is 50-100 mm; the width is 45-60 mm; the thickness is 2-6mm, the baffle of the box body is 10-30mm high and 1.5-2.5mm thick, the baffle of the transplant is 10-30mm high and 0.5-2.5mm thick, the horizontal handle is 45-60mm long and 5-20mm wide; the thickness is 5-13mm, the length of the arc-shaped groove is 45-60mm, the depth is 4-8mm, the length of the wide opening is 6-15 mm, the length of the cuboid upright column is 1-4mm, the width is 1-4mm, the height is 2-5mm, and the length of the rectangular groove is 40-50 mm; the width is 5-15 mm; the depth is 1.5-4.7 mm; the distance between adjacent rectangular grooves is 3-7mm, and the length of the scale is 40-50 mm; 1-3mm wide; the thickness is 0.1-0.7mm, the diameter of the circular drainage hole is 0.5-2mm, and the minimum unit of the scale mark of the scale is 0.05-3 mm;
The number of the rectangular grooves is 5, the number of the circular drain holes is 40, and the length of the box body is 90 mm; the width is 60 mm; the thickness is 2mm, and the length of the transplant is 80 mm; the width is 55 mm; the thickness is 5mm, the height of the baffle plate of the box body is 25mm, the thickness is 2mm, the height of the baffle plate of the transplant body is 20mm, the thickness is 1mm, and the length of the horizontal handle is 55 mm; the width is 15 mm; the thickness is 9mm, the length of the arc-shaped groove is 55mm, the depth is 7mm, the length of the wide opening is 11mm, the length of the cuboid upright column is 3mm, the width is 2mm, the height is 4mm, and the length of the rectangular groove is 45 mm; the width is 10 mm; depth 4.5 mm; the distance between adjacent rectangular grooves is 5mm, and the length of the scale is 45 mm; the width is 2 mm; the thickness is 0.3mm, the diameter of the circular drainage hole is 1mm, and the minimum unit of the scale mark of the scale is 0.1 mm.
10. A working method using the special device for tumor transplantation of zebra fish juvenile fish of claim 1, which comprises the following steps: inserting the transplant into the box body, transferring the zebra fish juvenile fish into the groove of the transplant by using a pipette, moving the transplant out of the box body, pouring out the liquid in the box body, inserting the transplant into the box body again, performing tumor transplantation by using a needle holder, and measuring the diameter of a spherical liquid drop formed after a culture solution containing tumor cells enters the juvenile fish.
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