CN110531069B - Strip-dividing multi-item combined immunofluorescence chromatography detection system - Google Patents
Strip-dividing multi-item combined immunofluorescence chromatography detection system Download PDFInfo
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- CN110531069B CN110531069B CN201910735105.4A CN201910735105A CN110531069B CN 110531069 B CN110531069 B CN 110531069B CN 201910735105 A CN201910735105 A CN 201910735105A CN 110531069 B CN110531069 B CN 110531069B
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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Abstract
The invention discloses a strip multi-item combined immunofluorescence chromatography detection system, which comprises: the device comprises a detection channel, a detection device and an automatic card abandoning and withdrawing device; the detection channels comprise more than one multi-item joint detection channel and more than two single-item detection channels, the multi-item joint detection channels comprise multiple detection cards and multiple card slots, the single detection channels comprise single detection cards and single card slots, the multiple detection cards and the single detection cards are respectively and movably arranged in the multiple card slots and the single card slot, and at least two detection strips are arranged in the multiple detection cards; the detection device comprises an excitation light source and a fluorescence detector. The detection method has the advantages of convenient and quick operation, sanitation, safety and high sensitivity during detection, can detect detection signals with different wavelengths simultaneously, and realizes the simultaneous detection of a plurality of items on one detection line.
Description
Technical Field
The invention relates to a detection system, belongs to the field of biomedical and in-vitro diagnosis medical equipment, and particularly relates to a strip multi-item combined immunofluorescence chromatography detection system.
Background
At present, the detector generally adopts single detection channel or a plurality of single detection channel cooperation single item to detect the card and use, need to divide time or tear the card open many times when carrying out multinomial joint detection, carries out the application of sample to each detection card again, if do not annotate sample and detection card during the application of sample, probably confuse the detection card of different samples, and the operation is comparatively loaded down with trivial details. Furthermore, the detection card after detection needs to be taken out manually, the operation of workers is increased, or an automatic card pushing device is arranged, but the pushing opening pushed out automatically is over the bottom, so that the detection card is stacked on the desktop and easily pollutes the desktop, the detection card possibly falls into the instrument after too many detection cards are stacked, meanwhile, the action of automatic ejection is overlarge, no storage device exists, the detection card flies randomly, and the noise is overlarge.
Disclosure of Invention
The invention aims to provide a strip-dividing multi-item combined immunofluorescence chromatography detection system, which overcomes the defects of a detector in the background technology.
Therefore, the invention adopts the following technical proposal,
a strip-wise multinomial combined immunofluorescence chromatography detection system comprising: the device comprises a detection channel, a detection device and an automatic card abandoning and withdrawing device; the detection channels comprise more than one multi-item joint detection channel and more than two single-item detection channels, the multi-item joint detection channels comprise a plurality of detection cards and a plurality of card slots, the single-item detection channels comprise single detection cards and single card slots, the plurality of detection cards are movably arranged in the plurality of card slots, the plurality of detection cards and the single detection card are respectively movably arranged in the plurality of card slots and the single card slot, and at least two detection strips are arranged in the plurality of detection cards; the detection device comprises an excitation light source and a fluorescence detector.
Preferably, the discard card automatic withdrawing device comprises a discard card base plate, a push plate assembly, a discard card transmission device and a push plate positioning sensor, wherein the discard card base plate is provided with a chute for the push plate assembly to slide, and the discard card transmission device comprises a discard card motor, a first driving wheel, a first idle wheel and a first belt; abandon card automatic withdrawing device and set up on the drive plate, the drive plate is installed on the bottom plate, push pedal positioning sensor is used for responding to push pedal assembly's position, after detection achievement, push pedal assembly resets to the positioning sensor position again.
Preferably, the excitation light source is a narrowband excitation light source.
Preferably, a channel bottom plate is arranged on the bottom plate and fixed on the bottom plate through a first support column, the channel bottom plate is provided with a detection channel and a detection channel conveying mechanism, the detection channel conveying mechanism drives the detection channel to move longitudinally, and the detection channel conveying mechanism is composed of a channel positioning sensor, a second slide rail, a second driving wheel, a second idle wheel, a second belt and a channel motor.
Preferably, be provided with the detection bottom plate on the passageway bottom plate, it fixes to detect the bottom plate through the second pillar on the passageway bottom plate, be provided with detection device drive mechanism and detection device on the detection bottom plate, detection device drive mechanism drives detection device lateral shifting, detection device drive mechanism comprises detection positioning sensor, third action wheel, third idler, third belt and detection motor.
Preferably, a computer host is further arranged on the bottom plate, and the computer host is connected with the fluorescence detector and is used for displaying and operating through an external touch display screen.
Preferably, a card detection sensor is arranged on the channel bottom plate, and after the card detection sensor senses multiple detection cards in the multiple card slots or a single detection card in the single card slot, the detection device moves to the multiple card slots or the single card slot under the action of the detection positioning sensor. Preferably, the channel bottom plate is provided with a leak hole matched with the multiple detection cards and the single detection card, and the multiple detection cards and the single detection card fall on the card abandoning bottom plate through the leak hole.
Preferably, the detection system has a print output function, a test result output function, and an operation error and mechanical failure prompt function.
Preferably, an ID card reader is arranged on the channel bottom plate, an ID card is arranged in the ID card reader, and the ID card is used for importing detection item information.
Compared with the background technology, the technical scheme has the following advantages:
1) when the multi-project joint detection is carried out, the multi-project joint detection card is more convenient and simpler to operate than a single detection card. Before sample adding, only one detection card needs to be removed, the detection card does not need to be removed for multiple times according to multiple joint detection items, and the operation is convenient. Only one detection card is needed for one sample, the sample is continuously added on the same detection card during sample adding, the sample adding operation on different detection cards is not needed, the operation is simple and convenient, and different samples can not be confused with the detection cards. During detection, the card is inserted only once, and repeated insertion or repeated insertion is not needed, so that the operation is convenient and quick.
2) The supporting automatic withdrawing means of card of abandoning need not the manual work after the detection achievement and gets the card, and the detection card after the automatic collection detects emptys once every day can, need not to get the card after the detection achievement at every turn, also need not to collect the detection card that the desktop scatters after the detection achievement, and too big noise has improved the stability and the repeatability of detector, and the possibility of pollution-free desktop, and is simple and convenient, sanitary safety.
3) By adopting the narrow-band excitation light source, excitation light does not need to pass through a light filter, so that light attenuation is reduced, the intensity of the excitation light is enhanced, the sensitivity of the detector is greatly improved, detection signals with different wavelengths can be detected simultaneously, and a plurality of items can be detected simultaneously on one detection line.
Drawings
FIG. 1 is a flow chart of the detection of a strip-dividing multi-item combined immunofluorescence chromatography detection system.
FIG. 2 is a three-dimensional structure diagram of a multi-sectional combined immunofluorescence chromatography detection system.
Fig. 3 is a structural diagram of an automatic card discarding withdrawing device.
Fig. 4 is a view of the construction of the tunnel floor.
Fig. 5 is a view showing the structure of the inspection substrate.
Detailed Description
In order that the objects, features and advantages of the invention will be readily apparent, a more particular description of embodiments of the invention will be rendered by reference to the appended drawings and examples, wherein specific details are set forth in order to provide a thorough understanding of the invention, but the invention may be practiced in other ways than as described. Accordingly, the invention is not limited by the specific implementations disclosed below.
Examples
As shown in fig. 1-5, a strip-by-strip multi-item combined immunofluorescence chromatography detection system comprises: a detection channel, a detection device 200 and a card abandoning automatic exit device 300; the detection channel 100 comprises more than one multi-item joint detection channel and more than two single-item detection channels, the multi-item joint detection channel comprises a plurality of detection cards and a plurality of card slots 102, the single-item detection channel comprises a single detection card and a single card slot 109, the plurality of detection cards and the single detection card are respectively and movably arranged in the plurality of card slots 102 and the single card slot 109, and at least two detection strips are arranged in the plurality of detection cards; the detection device 200 includes an excitation light source and a fluorescence detector. When a sample needs to be subjected to multi-project joint detection, only one multi-project detection card needs to be detached for sample adding, the sample is continuously added on the same detection card during sample adding, the sample adding operation on different detection cards is not needed, the operation is simple and convenient, and different samples and detection cards cannot be mixed.
The card discarding automatic withdrawing device 300 comprises a card discarding bottom plate 301, a push plate assembly, a card discarding transmission device, a push plate positioning sensor 302 and a waste material box, wherein a sliding groove 303 for the push plate assembly to slide is formed in the card discarding bottom plate 301, the push plate assembly comprises a push plate 304 and a push plate support 305, the push plate 304 is fixed on the push plate support 305, and the card discarding transmission device comprises a card discarding motor 306, a first driving wheel 307, a first idle wheel 308 and a first belt 309; the card abandoning automatic withdrawing device 300 is arranged on a drive plate 310, the drive plate 310 is arranged on a bottom plate 311, a push plate positioning sensor 302 is used for sensing the position of a push plate component, a waste material box is used for collecting the detection cards which are withdrawn by the card abandoning automatic withdrawing device, the waste material box is used for collecting the multiple detection cards and the single detection card which are withdrawn by the card abandoning automatic withdrawing device 300, and the push plate component resets to the position of the push plate positioning sensor 302 again after the collection of the multiple detection cards and the single detection card is completed. The multiple detection cards and the single detection card fall onto the card abandoning bottom plate 301 after detection work is finished, the push plate assembly is driven by the card abandoning transmission device to slide along the sliding groove 303 to push the multiple detection cards and the single detection card to the outside of the detection system, and the multiple detection cards and the single detection card fall into the waste material box. The card is not required to be checked manually, the possibility of polluting the desktop is avoided, and the method is simple, convenient, sanitary and safe.
The excitation light source is a narrow-band excitation light source, the excitation light source emits excitation light, the detected substance on the multiple detection cards or the single detection card emits fluorescence under the action of the excitation light, the fluorescence detector converts a fluorescence signal into an electric signal and calculates the electric signal, and the calculation result is sent to the detection software. Meanwhile, a narrow-band excitation light source is adopted, excitation light does not need to pass through a light filter, light attenuation is reduced, the intensity of the excitation light is enhanced, the sensitivity of the detector is greatly improved, detection signals with different wavelengths can be detected simultaneously, and multiple items can be detected on one detection line simultaneously.
Wherein, be provided with passageway bottom plate 101 on the bottom plate 311, passageway bottom plate 101 is fixed through first pillar 600 on the bottom plate 311, be equipped with on the passageway bottom plate 101 detection passageway and detection passageway transport mechanism, detection passageway transport mechanism drives detection passageway longitudinal motion, detection passageway transport mechanism comprises passageway positioning sensor 103, second slide rail 104, second action wheel 105, second idler 106, second belt 107 and passageway motor 108.
The detection device comprises a channel bottom plate 101, wherein a detection bottom plate 201 is arranged on the channel bottom plate 101, the detection bottom plate 201 is fixed on the channel bottom plate 101 through a second support column 700, a detection device transmission mechanism and a detection device 200 are arranged on the detection bottom plate 201, the detection device transmission mechanism drives the detection device 200 to move transversely, and the detection device transmission mechanism is composed of a detection positioning sensor 202, a third driving wheel 203, a third idle wheel 204, a third belt 205 and a detection motor 206.
The detection device 200 is moved laterally from the first position to the second position, and the detection channel is moved longitudinally from the third position to the fourth position, which, under the cooperation of the detection alignment sensor 202 and the channel alignment sensor 103, positions the detection device 200 directly above the plurality of detection cards and the single detection card. After the next boot, before the detection operation starts, the detection system is reset, the detection device 200 is reset to the first position, and the detection channel is reset to the third position.
The bottom plate 311 is further provided with a host computer, the host computer is connected with the fluorescence detector and is used for displaying and operating through an external touch display screen, and the operation including detection, historical data, project management and setting are all achieved through the external touch display screen. Meanwhile, the design of the detection software is very concise and humanized, and the detection software is suitable for being used in medical service points and central laboratories.
A card detection sensor 400 is arranged on the channel bottom plate 101, and after the card detection sensor 400 senses multiple detection cards in the multiple card slots 102 or single detection cards in the single card slot 109, the detection device 200 moves to the positions of the multiple card slots 102 or the single card slot 109 under the action of the detection positioning sensor 202 to detect the multiple detection cards or the single detection card; when a plurality of the multi-item test cards and the single test card are put in, the card detection sensor 400 detects the multi-item test cards and the single test card according to the order of putting the multi-item test cards and the single test card.
The channel bottom plate 101 is provided with a leak hole matched with the multiple detection cards and the single detection card, and the multiple detection cards and the single detection card fall on the card discarding bottom plate 301 through the leak hole.
Wherein the waste box can accommodate 200 single test cards or 30 test cards.
The detection system has a printing output function, a test result output function and an operation error and mechanical failure prompt function.
An ID card reader 500 is arranged on the channel bottom plate 101, and an ID card is arranged in the ID card reader 500 and used for importing detection item information.
Wherein, the bottom plate is also provided with a fan 800 for heat dissipation.
When the strip-type multi-item combined immunofluorescence chromatography detection system is used for detection, whole blood, serum or quality control products can be used for detection, and the detection is carried out according to the actual needs of each item. And inserting the multiple detection cards or the single detection card into the corresponding detection channel, starting the detection work of the detection system, displaying the detection result on the external touch display screen, simultaneously selecting corresponding information, and printing through the printing output function.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (8)
1. A strip-type multi-item combined immunofluorescence chromatography detection system is characterized by comprising: the device comprises a detection channel, a detection device and an automatic card abandoning exit device; the detection channels comprise more than one multi-item joint detection channel and more than two single-item detection channels, the multi-item joint detection channels comprise multiple detection cards and multiple card slots, the single detection channels comprise single detection cards and single card slots, the multiple detection cards and the single detection cards are respectively and movably arranged in the multiple card slots and the single card slot, and at least two detection strips are arranged in the multiple detection cards; the detection device comprises an excitation light source and a fluorescence detector; the detection device comprises a base plate, a detection channel conveying mechanism, a channel positioning sensor, a second slide rail, a second driving wheel, a second idle wheel, a second belt and a channel motor, wherein the base plate is provided with a channel base plate which is fixed on the base plate through a first support column; the detection device comprises a channel bottom plate, and is characterized in that a detection bottom plate is arranged on the channel bottom plate and fixed on the channel bottom plate through a second support column, a detection device transmission mechanism and a detection device are arranged on the detection bottom plate, the detection device transmission mechanism drives the detection device to transversely move, and the detection device transmission mechanism is composed of a detection positioning sensor, a third driving wheel, a third idle wheel, a third belt and a detection motor.
2. The strip-dividing multi-item combined immunofluorescence chromatography detection system according to claim 1, wherein the discard card automatic withdrawing device comprises a discard card base plate, a push plate assembly, a discard card transmission device and a push plate positioning sensor, a slide groove for the push plate assembly to slide is arranged on the discard card base plate, and the discard card transmission device comprises a discard card motor, a first driving wheel, a first idle wheel and a first belt; abandon card automatic withdrawing device and set up on the drive plate, the drive plate is installed on the bottom plate, push pedal positioning sensor is used for responding to push pedal assembly's position, after detection achievement, push pedal assembly resets to the positioning sensor position again.
3. The strip multi-item combined immunofluorescence chromatography detection system according to claim 1, wherein the excitation light source is a narrowband excitation light source.
4. The strip-dividing multi-item combined immunofluorescence chromatography detection system according to claim 2, wherein a host computer is further arranged on the bottom plate, and the host computer is connected with the fluorescence detector and used for displaying and operating through an external touch display screen.
5. The strip-dividing multi-item combined immunofluorescent chromatography detection system according to claim 1, wherein a card detection sensor is arranged on the channel bottom plate, and after the card detection sensor senses a plurality of detection cards in the plurality of card slots or a single detection card in the single card slot, the detection device moves to the positions of the plurality of card slots or the single card slot under the action of the detection positioning sensor.
6. The strip-dividing multi-item combined immunofluorescence chromatography detection system according to claim 2, wherein a leak hole matched with the multi-item detection cards and the single item detection cards is formed in the channel bottom plate, and the multi-item detection cards and the single item detection cards fall on the card discarding bottom plate through the leak hole.
7. The strip multi-item combined immunofluorescent chromatography detection system according to claim 1, wherein the detection system has a print-out function, a test result output function, and an operation error and mechanical failure prompt function.
8. The strip-dividing multi-item combined immunofluorescence chromatography detection system according to claim 1, wherein an ID card reader is arranged on the channel bottom plate, an ID card is arranged in the ID card reader, and the ID card is used for importing detection item information.
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