CN110506570A - A kind of ectotrophic mycorrhiza liquid thallus classification inoculation apparatus and method promoting Growth of Masson Pine - Google Patents

A kind of ectotrophic mycorrhiza liquid thallus classification inoculation apparatus and method promoting Growth of Masson Pine Download PDF

Info

Publication number
CN110506570A
CN110506570A CN201910753501.XA CN201910753501A CN110506570A CN 110506570 A CN110506570 A CN 110506570A CN 201910753501 A CN201910753501 A CN 201910753501A CN 110506570 A CN110506570 A CN 110506570A
Authority
CN
China
Prior art keywords
feed
tube
connector
masson pine
liquid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201910753501.XA
Other languages
Chinese (zh)
Other versions
CN110506570B (en
Inventor
张珍明
周鑫伟
周运超
张家春
贺红早
靳芙蓉
黄先飞
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guizhou University
Original Assignee
Guizhou University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guizhou University filed Critical Guizhou University
Priority to CN201910753501.XA priority Critical patent/CN110506570B/en
Publication of CN110506570A publication Critical patent/CN110506570A/en
Application granted granted Critical
Publication of CN110506570B publication Critical patent/CN110506570B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/50Inoculation of spawn

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a kind of ectotrophic mycorrhiza liquid thallus classification inoculation apparatus for promoting Growth of Masson Pine, including tube for feed, the bottom of tube for feed is fixedly connected with installation casing, transformer and rechargeable battery are installed inside installation casing, connecting partition on the inner wall of installation casing, partition is for separating transformer and rechargeable battery;Unidirectional air inlet valve is also equipped at the top of tube for feed.The present invention also proposes a kind of application method of ectotrophic mycorrhiza liquid thallus classification inoculation apparatus for promoting Growth of Masson Pine, includes the following steps;The first step, culture matrix manufacturing;Second step, inoculation;Third step, Spawn incubation;4th step, the root fungus liquid that reconciles;5th step, push button switch, so that booster pump is towards tube for feed plenum;6th step, the root that root fungus liquid is sprayed to tree.The root fungus liquid measure that the present invention carries is more, and root fungus liquid can be sprayed at bark notch on the edge of a knife to.And the method for the invention patent can realize the scale of masson pine breeding, industrialization production.

Description

A kind of ectotrophic mycorrhiza liquid thallus classification inoculation apparatus and method promoting Growth of Masson Pine
Technical field
The present invention relates to root fungus inoculation technique field more particularly to a kind of exotrophic mycorrhiza bacteria liquids for promoting Growth of Masson Pine Thallus classification inoculation apparatus and method.
Background technique
The root system of many macro fungis and higher plant forms symbiosis, we are known as exotrophic mycorrhiza.The formation of mycorhiza It is the very universal ecological phenomenon of nature.Ectotrophic mycorrhiza is that the mycorhiza of the root formation of fungi and higher plant is fit, host Make bidirectional movement by the network of Hartig net with the metabolite of VA Mycorrhizal Fungi.China have the Main tree of exotrophic mycorrhiza have oak, pine, Willow, linden, maple, English walnut and Betulaceae etc.;Many of mycorrhizal fungi is precious edible mushroom;During masson pine plantation, Root also long root fungus, for economic benefits, people are inoculated with root fungus in tree root, not only help the growth of trees in this way, moreover it is possible to bring Economic benefit, for this purpose, it is proposed that a kind of ectotrophic mycorrhiza liquid thallus classification inoculation apparatus and method for promoting Growth of Masson Pine.
Summary of the invention
The purpose of the present invention is to solve disadvantages existing in the prior art, and a kind of promotion Growth of Masson Pine proposed Ectotrophic mycorrhiza liquid thallus classification inoculation apparatus and method.
To achieve the goals above, present invention employs following technical solutions:
Design a kind of ectotrophic mycorrhiza liquid thallus classification inoculation apparatus for promoting Growth of Masson Pine, including tube for feed, which is characterized in that The bottom of tube for feed is fixedly connected with installation casing, and transformer and rechargeable battery are equipped with inside installation casing, installation casing Connecting partition on inner wall, partition is for separating transformer and rechargeable battery;One-way air inlet is also equipped at the top of tube for feed The top of valve, Unidirectional air inlet valve is connect with pressurization pump inlet, and booster pump is connected to tube for feed by motor fixing frame Top;
It further include a button switch, the output terminal of the transformer is connect by conducting wire with booster pump connecting terminal, button Power supply line is respectively connected on Switch output terminal and input terminal, a power supply line is connected with the terminals of rechargeable battery, another Root power supply line is connect with the input terminal of the transformer, and when push button switch, electric current passes sequentially through button switch, transformation Device and booster pump;
It further include a catheter, the both ends of catheter are respectively connected with the second connector, and second connector is connected to charge The side bottom of cylinder, another second connector are connected in the end sides of support tube body, are equipped on the support tube body Control valve is equipped with spray head far from one end of the second connector in support tube body.
Preferably, the side of tube for feed is the arcwall face structure coincideing with human body back, the tube for feed in arcwall face two sides Harness is equipped on side wall, harness includes shoulder belt, and one end and the tube for feed side top of shoulder belt are connected and fixed, and the other end passes through day The other end of button connection adjustment band, the adjustment band is fixed on the bottom of the tube for feed side;And at the top of tube for feed One end is equipped with inlet, passes through seal cap sealing on inlet.
Preferably, the power supply line is on the outside of tube for feed, and button switch is mounted on bar shaped handle, and on bar shaped handle It is also equipped with air gauge, barometric import is connected to by connecting conduit and the first connector, the other end of the first connector The top of tube for feed.
Preferably, spiral elastic pipes are in series on catheter.
Preferably, support tube body is coaxially mounted in support rod body close to one end of the second connector.
Preferably, the spray head includes third connector, and third connector is threaded on support tube body, and in third The end of connector is coaxially provided with the 4th connector, is threaded with syringe needle on the 4th connector;
The end of the third connector is located at installation inside pipe casing, and installs and be threadedly coupled between casing and third connector, Multiple quadrangular pilot holes are offered far from one end of support tube body in installation casing, quadrangular pilot hole is blind hole, quadrangular Pilot hole is equipped with compression spring, compression spring around the central axis annular array for installing casing, each quadrangular pilot hole One end through quadrangular pilot hole bottom to installation outside of sleeve and connection fixed thereto, at the end of each quadrangular pilot hole It is inserted with matching quadrangular guide rod at mouthful, quadrangular guide rod is fixedly connected with compression spring end, and quadrangular is led To the blade of the other end of bar connection bar shaped sheet, blade is arranged radially with installation set pipe;The edge of a knife of the blade is provided with Its end, and the length of blade is greater than the length of syringe needle, syringe needle is trapped among wherein by blade.
Preferably, the opening of installation casing is equipped with sealing plate, is connected by screw between sealing plate and installation casing, and And the charging port of rechargeable battery is exposed on the outside through sealing plate.
The present invention also proposes a kind of application method of ectotrophic mycorrhiza liquid thallus classification inoculation apparatus for promoting Growth of Masson Pine, Characterized by comprising the following steps:
The first step, culture matrix manufacturing: in parts by weight, 50-100 parts of red bolete Producing Area Soil, peeled potatoes 60- are taken 120 parts are cut into bulk, and red bolete Producing Area Soil and blocky potato are added in 1000 parts of water, 20~30min is boiled, uses yarn Cloth filters off soil and potato residue, white granulated sugar 8-10 parts of the addition into filtrate, and 0.4-0.06 parts of dipotassium hydrogen phosphate, calcium chloride 3-5 Part, 0.1-0.3 parts of magnesium sulfate, 0.3-0.5 parts of yeast powder, add water to be settled to 1000 parts, control pH value is 5.5-6.5;
Second step, inoculation: sterilising medium and inoculating tool are put into superclean bench, using ultraviolet lamp sterilizing 30-40min Afterwards, red bolete fructification is integrally wiped one time with the alcohol that cotton dips in 75% in superclean bench, then lights alcohol Vaccinating lancet is set alcolhol burner flame envelope calcination 1s by lamp, with the vaccinating lancet after calcination by red ox at alcolhol burner flame envelope front upper place 5-6cm Liver mushroom entity is divided into 3-4 and distinguishes, cap is taken to be put into the red of asepsis growth with the red bolete fructification tissue 1-3g in stem joint In bolete fluid nutrient medium;
Third step, Spawn incubation: inoculated culture medium is placed in 25-28 DEG C, in the Spawn incubation shaking table of 120-140r/min 6~8d is cultivated, the inoculation cultivation of next step can be carried out to cover with white hypha in culture medium.
4th step, the root fungus liquid that reconciles, are poured on tube for feed (1) for root fungus liquid and save, harness is dressed on people user's shoulder, Sealing cover (3) are tightened simultaneously, prevent gas leakage;
5th step, push button switch (11), so that booster pump (27) is towards tube for feed (1) plenum, so that tube for feed (1) Interior pressure becomes larger;
6th step, the hand-held supports body of rod (15) or support tube body (19) open control valve by spray head pressing on tree root surface (18), so that the root fungus liquid of tube for feed (1) is sprayed at the root of tree.
A kind of ectotrophic mycorrhiza liquid thallus classification inoculation apparatus and method promoting Growth of Masson Pine proposed by the present invention is beneficial Effect is: when in use, unlatching booster pump is tube for feed internal pressurization, and when spray head offsets with usage tree root, blade is against The surface of tree root, multiple blades are contacted with tree root surface simultaneously, and root fungus liquid is sprayed at blade from syringe needle and cut out by Open valve The edge of a knife in.The root fungus liquid measure that the present invention carries is more, and sprays conveniently, and be convenient for carrying, and root fungus liquid can be sprayed to bark notch It is sprinkled upon on the edge of a knife, has with tree root and preferably contact.The present invention has the characteristics that very prominent, present invention life compared with prior art The Pinus massoniana Seedlings reproduction technique of production is simple;Taking root only needs 6-8 days, shifts to an earlier date 2-3 days than conventional method;Growth is rapid, cuttage Height of seedling increases 15-30cm than conventional method up to 50-800cm after 1 year;Use is by red bolete Producing Area Soil in the present invention For cutting medium, can effectively increase Soil Microorganism improves soil physico-chemical property (porosity, aggregate, mineral nutrient etc.), Significantly improve masson pine rhizospheric microflora of soil.Meanwhile method of the invention is easy to operate, easy to spread, mentions than conventional method High soil porosity 3~8% improves soil agreegate 5~8%, supplements beneficial microbe to enhance the disease-resistant disease of masson pine, improves Soil physico-chemical property, balanced soil microecosystem improve the survival rate of masson pine, after afforestation survival rate up to 90% or more, than Conventional method improves 5-10%.The method of the invention patent can realize the scale of masson pine breeding, industrialization production.
Detailed description of the invention
Attached drawing is used to provide further understanding of the present invention, and constitutes part of specification, with reality of the invention It applies example to be used to explain the present invention together, not be construed as limiting the invention.In the accompanying drawings:
Fig. 1 is a kind of structural representation of ectotrophic mycorrhiza liquid thallus classification inoculation apparatus for promoting Growth of Masson Pine proposed by the present invention Figure one.
Fig. 2 is a kind of structure of ectotrophic mycorrhiza liquid thallus classification inoculation apparatus for promoting Growth of Masson Pine proposed by the present invention Schematic diagram two.
Fig. 3 is a kind of spray head of ectotrophic mycorrhiza liquid thallus classification inoculation apparatus for promoting Growth of Masson Pine proposed by the present invention Structural schematic diagram.
Fig. 4 is a kind of spray head of ectotrophic mycorrhiza liquid thallus classification inoculation apparatus for promoting Growth of Masson Pine proposed by the present invention The schematic diagram of the section structure.
Label is in figure: tube for feed 1, inlet 2, sealing cover 3, first connector 4, motor fixing frame 5, shoulder belt 6, Detain 7, adjustment band 8, connecting conduit 9, power supply line 10, button switch 11, air gauge 12, bar shaped handle 13, installation casing 14, branch day Strut body 15, spiral elastic pipes 16, the second connector 17, control valve 18, support tube body 19, third connector 20, installation set Pipe 21, transformer 22, partition 23, rechargeable battery 24, Unidirectional air inlet valve 25, sealing plate 26, booster pump 27, catheter 28, four Prism pilot hole 29, quadrangular guide rod 30, blade 31, syringe needle 32, the 4th connector 33, compression spring 34.
Specific embodiment
To make the objectives, technical solutions, and advantages of the present invention clearer, below with reference to embodiment and attached drawing, to this Invention is described in further detail, and exemplary embodiment of the invention and its explanation for explaining only the invention, are not made For limitation of the invention.
Now in conjunction with Figure of description, the design feature that the present invention will be described in detail.
Referring to Fig. 1-4, a kind of ectotrophic mycorrhiza liquid thallus classification inoculation apparatus promoting Growth of Masson Pine, including tube for feed 1, The bottom of tube for feed 1 is fixedly connected with installation casing 14, and transformer 22 and rechargeable battery 24 are equipped with inside installation casing 14, peace Connecting partition 23 on the inner wall of vanning body 14, partition 23 is for separating transformer 22 and rechargeable battery 24;The top of tube for feed 1 Portion is also equipped with Unidirectional air inlet valve 25, and the top of Unidirectional air inlet valve 25 is connect with 27 import of booster pump, and booster pump 27 is logical The top that motor fixing frame 5 is connected to tube for feed 1 is crossed, when booster pump 27 works, the pressure in tube for feed 1 becomes larger;
The invention also includes a button switch 11, the output terminal of the transformer 22 passes through conducting wire and 27 terminals of booster pump Son connects, and is respectively connected with power supply line 10, a power supply line 10 and rechargeable battery on 11 output terminal of button switch and input terminal 24 terminals are connected, and another power supply line 10 is connect with the input terminal of the transformer 22, when push button switch 11, Electric current passes sequentially through button switch 11, transformer 22 and booster pump 27;Press on-off of the button switch 11 for control circuit;
The invention also includes a catheter 28, the both ends of catheter 28 are respectively connected with the second connector 17, one second connection First 17 are connected to the side bottom of tube for feed 1, another second connector 17 is connected in the end sides of support tube body 19, institute It states and control valve 18 is installed on support tube body 19, spray head is installed far from one end of the second connector 17 in support tube body 19.
Further, as shown in Figure 1, the side of tube for feed 1 is the arcwall face structure coincideing with human body back, in arcwall face Harness is equipped on 1 side wall of tube for feed of two sides, harness includes shoulder belt 6, and one end of shoulder belt 6 is connect with 1 side top of tube for feed Fixed, the other end is fixed on the bottom of 1 side of tube for feed by 7 connection adjustment band 8 of day button, the other end of the adjustment band 8 Portion;And it is equipped with inlet 2 in top one end of tube for feed 1, is sealed on inlet 2 by sealing cover 3.
Shoulder belt can adjust the length of shoulder belt using 7 connection of day button at any time;
Further, as shown in Fig. 2, the power supply line 10 is in 1 outside of tube for feed, and button switch 11 is mounted on bar shaped handle 13, and air gauge 12 is also equipped on bar shaped handle 13, the import of air gauge 12 passes through connecting conduit 9 and the first connector 4, The other end of first connector 4 is connected to the top of tube for feed 1.
User can hold bar shaped handle 13, prevent holding in the palm of the hand, and thumb, which facilitates, presses button switch 11, simultaneously The air pressure in tube for feed 1 can be observed constantly, and when reaching preset pressure, big thumb is unclamped from button switch 11.
Further, spiral elastic pipes 16 are in series on catheter 28, spiral elastic pipes 16 can extend, and can be automatic The function of contraction can increase length when using, and be that can retract when not using, and reduce length.
Further, support tube body 19 is coaxially mounted in support rod body 15 close to one end of the second connector 17, set The support rod body 15 set is convenient for users to two-handed hand-held.
Further, the spray head includes third connector 20, and third connector 20 is threaded on support tube body 19, And it is coaxially provided with the 4th connector 33 in the end of third connector 20, syringe needle 32, needle are threaded on the 4th connector 33 First 32 for puncturing the epidermis of tree root;
The end of the third connector 20 is located inside installation casing 21, and installs spiral shell between casing 21 and third connector 20 Line connection offers multiple quadrangular pilot holes 29, quadrangular pilot hole far from one end of support tube body 19 in installation casing 21 29 be blind hole, and quadrangular pilot hole 29 is around the central axis annular array of installation casing 21, and each quadrangular pilot hole 29 is Equipped with compression spring 34, as at arrow B meaning, one end of compression spring 34 is through 29 bottom of quadrangular pilot hole to peace in Fig. 4 21 outside of tubing and connection fixed thereto, are inserted with matching quadrangular at the port of each quadrangular pilot hole 29 Guide rod 30, quadrangular guide rod 30 are fixedly connected with 34 end of compression spring, the other end connection strap of quadrangular guide rod 30 The blade 31 of shape sheet, blade 31 are arranged radially with installation casing 21;The edge of a knife of the blade 31 is provided with its end, and The length of blade 31 is greater than the length of syringe needle 32, and syringe needle 32 is trapped among wherein by blade 31.
Specifically, when in use, when spray head offsets with usage tree root, blade 31 is against the surface of tree root, multiple blades 31 contact with tree root surface simultaneously, so that the pattern suppressed is the hole that centre is 32 Zha Chu of syringe needle, and the knife that blade 31 is cut out Opening's edge 32 Zha Chu of syringe needle hole it is arranged in a ring so that being sprayed at the edge of a knife that blade 31 cuts out from the root fungus liquid that syringe needle 32 sprays In.
Further, the opening of installation casing 14 is equipped with sealing plate 26, passes through between sealing plate 26 and installation casing 14 Screw connection, and the charging port of rechargeable battery 24 is exposed on the outside through sealing plate 26.
The present invention also proposes a kind of application method of ectotrophic mycorrhiza liquid thallus classification inoculation apparatus for promoting Growth of Masson Pine, It is characterized by comprising the following steps;
The first step, culture matrix manufacturing: in parts by weight, 50-100 parts of red bolete Producing Area Soil, peeled potatoes 60- are taken 120 parts are cut into bulk, and red bolete Producing Area Soil and blocky potato are added in 1000 parts of water, 20~30min is boiled, uses yarn Cloth filters off soil and potato residue, white granulated sugar 8-10 parts of the addition into filtrate, and 0.4-0.06 parts of dipotassium hydrogen phosphate, calcium chloride 3-5 Part, 0.1-0.3 parts of magnesium sulfate, 0.3-0.5 parts of yeast powder, add water to be settled to 1000 parts, control pH value is 5.5-6.5;
Second step, inoculation: sterilising medium and inoculating tool are put into superclean bench, using ultraviolet lamp sterilizing 30-40min Afterwards, red bolete fructification is integrally wiped one time with the alcohol that cotton dips in 75% in superclean bench, then lights alcohol Vaccinating lancet is set alcolhol burner flame envelope calcination 1s by lamp, with the vaccinating lancet after calcination by red ox at alcolhol burner flame envelope front upper place 5-6cm Liver mushroom entity is divided into 3-4 and distinguishes, cap is taken to be put into the red of asepsis growth with the red bolete fructification tissue 1-3g in stem joint In bolete fluid nutrient medium;
Third step, Spawn incubation: inoculated culture medium is placed in 25-28 DEG C, in the Spawn incubation shaking table of 120-140r/min 6~8d is cultivated, the inoculation cultivation of next step can be carried out to cover with white hypha in culture medium.
4th step, the root fungus liquid that reconciles, are poured on tube for feed (1) for root fungus liquid and save, harness is dressed on people user's shoulder, Sealing cover (3) are tightened simultaneously, prevent gas leakage;
5th step, push button switch (11), so that booster pump (27) is towards tube for feed (1) plenum, so that tube for feed (1) Interior pressure becomes larger;
6th step, the hand-held supports body of rod (15) or support tube body (19) open control valve by spray head pressing on tree root surface (18), so that the root fungus liquid of tube for feed (1) is sprayed at the root of tree.
Tree root table can be carried out incision treatment, root fungus liquid is preferably connect with tree root by scheme proposed by the present invention Touching.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto, Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.

Claims (8)

1. a kind of ectotrophic mycorrhiza liquid thallus classification inoculation apparatus for promoting Growth of Masson Pine, including tube for feed (1), feature exists In the bottom of tube for feed (1) is fixedly connected with installation casing (14), is equipped with transformer (22) inside installation casing (14) and is filled Battery (24), connecting partition (23) on the inner wall of installation casing (14), partition (23) are used for transformer (22) and charging is electric Pond (24) separates;Be also equipped at the top of tube for feed (1) Unidirectional air inlet valve (25), the top of Unidirectional air inlet valve (25) with Booster pump (27) import connection, and booster pump (27) is connected to the top of tube for feed (1) by motor fixing frame (5);
It further include a button switch (11), the output terminal of the transformer (22) passes through conducting wire and booster pump (27) terminals Son connection, is respectively connected with power supply line (10) on button switch (11) output terminal and input terminal, a power supply line (10) with fill The terminals of battery (24) are connected, and another power supply line (10) connect with the input terminal of the transformer (22), works as pressing When button switch (11), electric current passes sequentially through button switch (11), transformer (22) and booster pump (27);
It further include a catheter (28), the both ends of catheter (28) are respectively connected with the second connector (17), one second connection Head (17) is connected to the side bottom of tube for feed (1), another second connector (17) is connected to the end of support tube body (19) On side, control valve (18) are installed on the support tube body (19), in support tube body (19) far from the second connector (17) One end spray head is installed.
2. a kind of ectotrophic mycorrhiza liquid thallus classification inoculation apparatus for promoting Growth of Masson Pine according to claim 1, special Sign is that the side of tube for feed (1) is the arcwall face structure coincideing with human body back, in tube for feed (1) side of arcwall face two sides Harness is equipped on wall, harness includes shoulder belt (6), and one end and tube for feed (1) side top of shoulder belt (6) are connected and fixed, another End is fixed on the bottom of the tube for feed (1) side by day button (7) connection adjustment band (8), the other end of the adjustment band (8) Portion;And inlet (2) are equipped in top one end of tube for feed (1), are sealed on inlet (2) by sealing cover (3).
3. a kind of ectotrophic mycorrhiza liquid thallus classification inoculation apparatus for promoting Growth of Masson Pine according to claim 1, special Sign is that the power supply line (10) is on the outside of tube for feed (1), and button switch (11) is mounted on bar shaped handle (13), and in item It is also equipped on shape handle (13) air gauge (12), the import of air gauge (12) passes through connecting conduit (9) and the first connector (4), the other end of the first connector (4) is connected to the top of tube for feed (1).
4. a kind of ectotrophic mycorrhiza liquid thallus classification inoculation apparatus for promoting Growth of Masson Pine according to claim 1, special Sign is, spiral elastic pipes (16) are in series on catheter (28).
5. a kind of ectotrophic mycorrhiza liquid thallus classification inoculation apparatus for promoting Growth of Masson Pine according to claim 1, special Sign is that support tube body (19) is coaxially mounted on support rod body (15) close to the one end of the second connector (17).
6. a kind of ectotrophic mycorrhiza liquid thallus classification inoculation apparatus for promoting Growth of Masson Pine according to claim 1, special Sign is that the spray head includes third connector (20), and third connector (20) is threaded in support tube body (19), and The end of third connector (20) is coaxially provided with the 4th connector (33), is threaded with syringe needle on the 4th connector (33) (32);
It is internal that the end of the third connector (20) is located at installation casing (21), and installs casing (21) and third connector (20) it is threadedly coupled between, offers multiple quadrangular pilot holes far from one end of support tube body (19) in installation casing (21) (29), quadrangular pilot hole (29) is blind hole, central axis ring row of the quadrangular pilot hole (29) around installation casing (21) Column, each quadrangular pilot hole (29) are equipped with compression spring (34), and quadrangular pilot hole is run through in one end of compression spring (34) (29) bottom is inserted at the port of each quadrangular pilot hole (29) to casing (21) outside and connection fixed thereto is installed Matching quadrangular guide rod (30), quadrangular guide rod (30) are fixedly connected with compression spring (34) end, quadrangular The blade (31) of the other end connection bar shaped sheet of guide rod (30), blade (31) are arranged radially with installation casing (21);Institute The edge of a knife for stating blade (31) is provided with its end, and the length of blade (31) is greater than the length of syringe needle 32, and blade (31) is by needle First 32 are trapped among wherein.
7. a kind of ectotrophic mycorrhiza liquid thallus classification inoculation apparatus for promoting Growth of Masson Pine according to claim 1, special Sign is that the opening of installation casing (14) is equipped with sealing plate (26), passes through spiral shell between sealing plate (26) and installation casing (14) Nail connection, and the charging port of rechargeable battery (24) is exposed on the outside through sealing plate (26).
8. a kind of ectotrophic mycorrhiza liquid thallus classification inoculation apparatus of promotion Growth of Masson Pine described in -6 makes according to claim 1 With method, which comprises the following steps:
The first step, culture matrix manufacturing: in parts by weight, 50-100 parts of red bolete Producing Area Soil, peeled potatoes 60- are taken 120 parts are cut into bulk, and red bolete Producing Area Soil and blocky potato are added in 1000 parts of water, 20~30min is boiled, uses yarn Cloth filters off soil and potato residue, white granulated sugar 8-10 parts of the addition into filtrate, and 0.4-0.06 parts of dipotassium hydrogen phosphate, calcium chloride 3-5 Part, 0.1-0.3 parts of magnesium sulfate, 0.3-0.5 parts of yeast powder, add water to be settled to 1000 parts, control pH value is 5.5-6.5;
Second step, inoculation: sterilising medium and inoculating tool are put into superclean bench, using ultraviolet lamp sterilizing 30-40min Afterwards, red bolete fructification is integrally wiped one time with the alcohol that cotton dips in 75% in superclean bench, then lights alcohol Vaccinating lancet is set alcolhol burner flame envelope calcination 1s by lamp, with the vaccinating lancet after calcination by red ox at alcolhol burner flame envelope front upper place 5-6cm Liver mushroom entity is divided into 3-4 and distinguishes, cap is taken to be put into the red of asepsis growth with the red bolete fructification tissue 1-3g in stem joint In bolete fluid nutrient medium;
Third step, Spawn incubation: inoculated culture medium is placed in 25-28 DEG C, in the Spawn incubation shaking table of 120-140r/min 6~8d is cultivated, the inoculation cultivation of next step can be carried out to cover with white hypha in culture medium;
4th step, the root fungus liquid that reconciles, are poured on tube for feed (1) for root fungus liquid and save, harness is dressed on people user's shoulder, simultaneously Sealing cover (3) are tightened, gas leakage is prevented;
5th step, push button switch (11), so that booster pump (27) is towards tube for feed (1) plenum, so that tube for feed (1) Interior pressure becomes larger;
6th step, the hand-held supports body of rod (15) or support tube body (19) open control valve by spray head pressing on tree root surface (18), so that the root fungus liquid of tube for feed (1) is sprayed at the root of tree.
CN201910753501.XA 2019-08-15 2019-08-15 Exophiala mycorrhizal fungi liquid thallus inoculation device and method for promoting growth of masson pine Active CN110506570B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201910753501.XA CN110506570B (en) 2019-08-15 2019-08-15 Exophiala mycorrhizal fungi liquid thallus inoculation device and method for promoting growth of masson pine

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201910753501.XA CN110506570B (en) 2019-08-15 2019-08-15 Exophiala mycorrhizal fungi liquid thallus inoculation device and method for promoting growth of masson pine

Publications (2)

Publication Number Publication Date
CN110506570A true CN110506570A (en) 2019-11-29
CN110506570B CN110506570B (en) 2021-09-14

Family

ID=68626059

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201910753501.XA Active CN110506570B (en) 2019-08-15 2019-08-15 Exophiala mycorrhizal fungi liquid thallus inoculation device and method for promoting growth of masson pine

Country Status (1)

Country Link
CN (1) CN110506570B (en)

Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103814755A (en) * 2014-03-09 2014-05-28 贵州省生物研究所 Manufacturing method of purple puffball liquid strain
CN204888460U (en) * 2015-06-16 2015-12-23 江西正米科技有限公司 Agricultural spraying apparatus
CN204907269U (en) * 2015-09-16 2015-12-30 黑龙江省森林保护研究所 Pinus sylvestris var. mongolica ectomycorrhiza fungus vaccinates rifle fast
CN205143305U (en) * 2015-12-02 2016-04-13 黑龙江志远环保科技有限公司 Biological fungus sprinkler
CN205233278U (en) * 2015-12-22 2016-05-18 芜湖禾沐农业科技有限公司 Medicine device is spouted to convenient handy agricultural
CN106134769A (en) * 2016-07-20 2016-11-23 贵州师范大学 A kind of manufacture method of Boletus edulis Bull ex Franch original seed
CN106258531A (en) * 2015-05-20 2017-01-04 裕元环境科学有限公司 Hermetic type pressure type trunk infusion appliance
CN206565138U (en) * 2017-01-19 2017-10-20 普洱林润经贸有限公司 A kind of quantitative chemical spraying device planted for coffee
CN208228169U (en) * 2018-05-03 2018-12-14 莫凯迪 A kind of electric disinsection device
CN208549334U (en) * 2018-05-02 2019-03-01 中国热带农业科学院环境与植物保护研究所 A kind of flower bud bud syringe for preventing and treating banana thrips
CN109418246A (en) * 2017-08-24 2019-03-05 海门市卓维纺织品有限公司 A kind of manual liquid medicine machine of agricultural
CN208925042U (en) * 2018-08-24 2019-06-04 赣南师范大学 A kind of citrus plantation root automatic ration device for administration of drugs

Patent Citations (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103814755A (en) * 2014-03-09 2014-05-28 贵州省生物研究所 Manufacturing method of purple puffball liquid strain
CN106258531A (en) * 2015-05-20 2017-01-04 裕元环境科学有限公司 Hermetic type pressure type trunk infusion appliance
CN204888460U (en) * 2015-06-16 2015-12-23 江西正米科技有限公司 Agricultural spraying apparatus
CN204907269U (en) * 2015-09-16 2015-12-30 黑龙江省森林保护研究所 Pinus sylvestris var. mongolica ectomycorrhiza fungus vaccinates rifle fast
CN205143305U (en) * 2015-12-02 2016-04-13 黑龙江志远环保科技有限公司 Biological fungus sprinkler
CN205233278U (en) * 2015-12-22 2016-05-18 芜湖禾沐农业科技有限公司 Medicine device is spouted to convenient handy agricultural
CN106134769A (en) * 2016-07-20 2016-11-23 贵州师范大学 A kind of manufacture method of Boletus edulis Bull ex Franch original seed
CN206565138U (en) * 2017-01-19 2017-10-20 普洱林润经贸有限公司 A kind of quantitative chemical spraying device planted for coffee
CN109418246A (en) * 2017-08-24 2019-03-05 海门市卓维纺织品有限公司 A kind of manual liquid medicine machine of agricultural
CN208549334U (en) * 2018-05-02 2019-03-01 中国热带农业科学院环境与植物保护研究所 A kind of flower bud bud syringe for preventing and treating banana thrips
CN208228169U (en) * 2018-05-03 2018-12-14 莫凯迪 A kind of electric disinsection device
CN208925042U (en) * 2018-08-24 2019-06-04 赣南师范大学 A kind of citrus plantation root automatic ration device for administration of drugs

Also Published As

Publication number Publication date
CN110506570B (en) 2021-09-14

Similar Documents

Publication Publication Date Title
CN103598010A (en) Original ecological imitative wild cultivation method for inonotus sanghuang
CN107135828B (en) A kind of peanut sclerotium rolfsii greenhouse Seedling Inoculation method
CN103787768A (en) Edible mushroom parent strain culture medium and preparation method thereof
CN108684440A (en) A method of cultivating coarse wool fibre pore fungi using rice
CN103493685A (en) Method for cultivating phellinus igniarius by using wild living tree stumps
CN209390742U (en) A kind of tree living of dendrobium candidum possesses a person culture apparatus
CN201709204U (en) Concave soilless culture bed for plants
CN110506570A (en) A kind of ectotrophic mycorrhiza liquid thallus classification inoculation apparatus and method promoting Growth of Masson Pine
CN205794035U (en) A kind of seedlings nursing plate seeder
CN106105774A (en) A kind of breeding method of high-yield flammulina velutipes
CN208783446U (en) A kind of comprehensive soilless cultivation frame collecting water planting and substrate culture
CN109845516A (en) A kind of device and method for breeding blueberry specificity symbiosis mycorrhiza fungi
CN104446822A (en) Special culture medium for oudemansiella canarri (jungh.) hohnel and oudemansiella canarri (jungh.) hohnel cultivation method
CN105393791A (en) Preparation method for living grafted Ganodermalucidum bonsais
CN207574168U (en) Intelligent multi-function Pleurotus eryngii bacteria stick culture apparatus
CN207574159U (en) Multi-functional Pleurotus eryngii culture bacterium germination Spawn incubation system
CN207411028U (en) New anti-knotting Pleurotus eryngii culture bacteria stick bag
CN206728649U (en) A kind of family mushroom culture
CN1771780A (en) Hunaigu mushroom sclerotium cultivating method and cultivating medium
CN105830839B (en) A method of tung oil tree fruit yield is improved by hormone induction
CN105519348A (en) A culture medium stick-shaped cultivation method for lentinula edodes
CN219330193U (en) Clean workbench for Chinese herbal medicine seedling breeding
CN220307790U (en) Tea garden biogas slurry efficient irrigation system
CN109168963A (en) A kind of method for cultivating mushroom of shading culture and both ends fruiting
CN213245703U (en) Rosemary is cultivated and uses sprinkler

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant