CN110506114A - Pcr引物对及其应用 - Google Patents

Pcr引物对及其应用 Download PDF

Info

Publication number
CN110506114A
CN110506114A CN201780089157.8A CN201780089157A CN110506114A CN 110506114 A CN110506114 A CN 110506114A CN 201780089157 A CN201780089157 A CN 201780089157A CN 110506114 A CN110506114 A CN 110506114A
Authority
CN
China
Prior art keywords
primer
pcr
amplification
sequence
random sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201780089157.8A
Other languages
English (en)
Other versions
CN110506114B (zh
Inventor
杨林
高雅
黄国栋
王逸从
王雨倩
张艳艳
陈芳
钟娜
蒋慧
徐讯
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
MGI Tech Co Ltd
Original Assignee
MGI Tech Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by MGI Tech Co Ltd filed Critical MGI Tech Co Ltd
Publication of CN110506114A publication Critical patent/CN110506114A/zh
Application granted granted Critical
Publication of CN110506114B publication Critical patent/CN110506114B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

提供了PCR引物对。其中，该PCR引物对包括：第一引物和第二引物，其中，所述第一引物包含第一特异性序列和第一随机序列，所述第一特异性序列位于所述第一引物的3’端，所述第一随机序列位于所述第一引物的5’端，所述第二引物包含第二特异性序列和第二随机序列，所述第二特异性序列位于所述第二引物的3’端，所述第二随机序列位于所述第二引物的5’端，并且，所述第一特异性序列和所述第二特异性序列分别为针对靶序列的上游引物和下游引物，所述第一随机序列和所述第二随机序列反向互补。

Description

PCT国内申请，说明书已公开。

Claims (14)

  1. PCT国内申请，权利要求书已公开。
CN201780089157.8A 2017-06-20 2017-06-20 Pcr引物对及其应用 Active CN110506114B (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2017/089195 WO2018232594A1 (zh) 2017-06-20 2017-06-20 Pcr引物对及其应用

Publications (2)

Publication Number Publication Date
CN110506114A true CN110506114A (zh) 2019-11-26
CN110506114B CN110506114B (zh) 2023-12-15

Family

ID=64735450

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201780089157.8A Active CN110506114B (zh) 2017-06-20 2017-06-20 Pcr引物对及其应用

Country Status (4)

Country Link
US (1) US11760995B2 (zh)
EP (1) EP3643788A4 (zh)
CN (1) CN110506114B (zh)
WO (1) WO2018232594A1 (zh)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113088561A (zh) * 2019-12-23 2021-07-09 深圳华大智造科技有限公司 构建侧翼已知序列的测序文库的引物组和方法

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11760995B2 (en) * 2017-06-20 2023-09-19 Mgi Tech Co., Ltd. PCR primer pair and application thereof
CN110029147B (zh) * 2019-01-25 2023-03-24 上海何因生物科技有限公司 一种单管实现连续区域扩增同时降低非特异性扩增的多重pcr方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060292611A1 (en) * 2005-06-06 2006-12-28 Jan Berka Paired end sequencing
US20090130720A1 (en) * 2001-01-19 2009-05-21 General Electric Company Methods and kits for reducing non-specific nucleic acid amplification
CN103820561A (zh) * 2014-03-10 2014-05-28 广西壮族自治区农业科学院园艺研究所 一种柑橘黄龙病亚洲种巢氏pcr扩增检测体系及应用
CN106282173A (zh) * 2015-05-27 2017-01-04 中国农业科学院生物技术研究所 克隆转基因生物中外源基因整合位点处侧翼序列的方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6218529B1 (en) * 1995-07-31 2001-04-17 Urocor, Inc. Biomarkers and targets for diagnosis, prognosis and management of prostate, breast and bladder cancer
AU5599101A (en) * 2000-05-09 2001-11-20 Diatech Pty Ltd Methods for detecting nucleic acid molecules having particular nucleotide sequences
CA2533119A1 (en) * 2003-07-24 2005-02-03 Qiagen Gmbh Method for the reverse transcription and/or amplification of nucleic acids
JP5455243B2 (ja) * 2008-06-18 2014-03-26 独立行政法人理化学研究所 未知の配列を含む増幅した二本鎖核酸の混合物を製造する方法
US20110195457A1 (en) * 2010-02-09 2011-08-11 General Electric Company Isothermal amplification of nucleic acid using primers comprising a randomized sequence and specific primers and uses thereof
CN102952895B (zh) * 2011-08-23 2014-12-17 中国科学院上海生命科学研究院 一种利用测序技术检测未知病毒的方法
EP2912197B1 (en) * 2012-10-24 2019-08-07 Takara Bio USA, Inc. Template switch-based methods for producing a product nucleic acid
AU2015210705B2 (en) * 2014-01-31 2020-11-05 Integrated Dna Technologies, Inc. Improved methods for processing DNA substrates
EP3643787A4 (en) * 2017-06-20 2021-01-06 MGI Tech Co., Ltd. PCR PRIMER PAIR AND USE OF IT
US11760995B2 (en) * 2017-06-20 2023-09-19 Mgi Tech Co., Ltd. PCR primer pair and application thereof
WO2018232598A1 (zh) * 2017-06-20 2018-12-27 深圳华大智造科技有限公司 Pcr引物对及其应用

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090130720A1 (en) * 2001-01-19 2009-05-21 General Electric Company Methods and kits for reducing non-specific nucleic acid amplification
US20060292611A1 (en) * 2005-06-06 2006-12-28 Jan Berka Paired end sequencing
CN103820561A (zh) * 2014-03-10 2014-05-28 广西壮族自治区农业科学院园艺研究所 一种柑橘黄龙病亚洲种巢氏pcr扩增检测体系及应用
CN106282173A (zh) * 2015-05-27 2017-01-04 中国农业科学院生物技术研究所 克隆转基因生物中外源基因整合位点处侧翼序列的方法

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CHRISTIAN KORFHAGE: "Clonal rolling circle amplification for on-chip DNA cluster generation", 《BIOL METHODS PROTOC》 *
CHRISTIAN KORFHAGE: "Clonal rolling circle amplification for on-chip DNA cluster generation", 《BIOL METHODS PROTOC》, vol. 2, no. 1, 1 January 2017 (2017-01-01), pages 2, XP055675828, DOI: 10.1093/biomethods/bpx007 *
HUANTING LIU等: "An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol", BMC BIOTECHNOL, vol. 8, no. 91, pages 1 - 10 *
JURATE BITINAITE: "USER™ friendly DNA engineering and cloning method by uracil excision", NUCLEIC ACIDS RES, vol. 35, no. 6, pages 1992 - 2002, XP055170276, DOI: 10.1093/nar/gkm041 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113088561A (zh) * 2019-12-23 2021-07-09 深圳华大智造科技有限公司 构建侧翼已知序列的测序文库的引物组和方法
CN113088561B (zh) * 2019-12-23 2024-05-14 深圳华大智造科技股份有限公司 构建侧翼已知序列的测序文库的引物组和方法

Also Published As

Publication number Publication date
WO2018232594A1 (zh) 2018-12-27
EP3643788A1 (en) 2020-04-29
CN110506114B (zh) 2023-12-15
US11760995B2 (en) 2023-09-19
US20200131510A1 (en) 2020-04-30
EP3643788A4 (en) 2021-01-06

Similar Documents

Publication Publication Date Title
US10683498B2 (en) Methods for generating circular DNA from circular RNA
DK3192877T3 (en) VESICULAR ADAPTERS AND APPLICATIONS THEREOF IN NUCLEIC ACID LIBRARY CONSTRUCTION AND SEQUENCE
Jalali et al. The polymerase chain reaction: PCR, qPCR, and RT-PCR
US20240052408A1 (en) Single end duplex dna sequencing
EP2272976A1 (en) Method for differentiation of polynucleotide strands
Dames et al. Comparison of the Illumina Genome Analyzer and Roche 454 GS FLX for resequencing of hypertrophic cardiomyopathy-associated genes
JP2019518476A (ja) 等温核酸自己増幅法
JP2023519782A (ja) 標的化された配列決定の方法
CN110506114A (zh) Pcr引物对及其应用
CN110734967A (zh) 一种接头组合物及其应用
JP2020536525A (ja) プローブ及びこれをハイスループットシーケンシングに適用するターゲット領域の濃縮方法
US20170362623A1 (en) Amplification of nucleic acids
JP4860199B2 (ja) 高度好熱菌由来の改変型一本鎖ｄｎａ結合タンパク質、及び該タンパク質を用いた核酸の等温増幅方法
CN113574181A (zh) 用于rna直接建库的核酸序列、基于rna样本直接构建测序文库的方法及应用
Etheridge et al. Preparation of small RNA NGS libraries from biofluids
CN116043337A (zh) Dna甲基化标志物筛查试剂盒及方法
EP3559268A1 (en) Methods and reagents for molecular barcoding
US11739319B2 (en) PCR primer pair and application thereof
US20090305288A1 (en) Methods for amplifying nucleic acids and for analyzing nucleic acids therewith
CN110612355A (zh) 用于定量pcr扩增的组合物及其应用
Nye et al. Chimeric DNA byproducts in strand displacement amplification using the T7 replisome
US20230122979A1 (en) Methods of sample normalization
Pogulis et al. Recombination and mutagenesis by overlap extension PCR
EP4259826A1 (en) Methods for sequencing polynucleotide fragments from both ends
CA3183405A1 (en) Isolation and immobilization of nucleic acids and uses thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
CB02 Change of applicant information

Address after: 518083 the comprehensive building of Beishan industrial zone and 11 2 buildings in Yantian District, Shenzhen, Guangdong.

Applicant after: Shenzhen Huada Zhizao Technology Co.,Ltd.

Address before: 518083 the comprehensive building of Beishan industrial zone and 11 2 buildings in Yantian District, Shenzhen, Guangdong.

Applicant before: MGI TECH Co.,Ltd.

CB02 Change of applicant information
GR01 Patent grant
GR01 Patent grant