CN110487766A - Fluorescence microscope observes living cells device in real time - Google Patents
Fluorescence microscope observes living cells device in real time Download PDFInfo
- Publication number
- CN110487766A CN110487766A CN201910826119.7A CN201910826119A CN110487766A CN 110487766 A CN110487766 A CN 110487766A CN 201910826119 A CN201910826119 A CN 201910826119A CN 110487766 A CN110487766 A CN 110487766A
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- China
- Prior art keywords
- real time
- living cells
- support arm
- fluorescence microscope
- cell
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
Abstract
The present invention relates to a kind of fluorescence microscopes to observe living cells device in real time.The fluorescence microscope observes living cells device, including pedestal in real time, the cell incubator being mounted on the base, and the top transparent of cell incubator is so that light passes through；The observation assembly for the real-time observation cell being mounted on the base；Observation assembly includes camera lens, support arm, the regulating mechanism for driving support arm to move back and forth, and optical system is equipped in support arm, and optical system and camera lens cooperate and is connected with control system.The top transparent of cell incubator, the observation assembly for not needing will to be used for observe cell in real time are arranged inside cell incubator, cell and observation device are separated, it can the state of observation living cells in real time, and will not pair or cell generation adverse effect.
Description
Technical field
The invention belongs to microscope technology fields, and in particular to a kind of fluorescence microscope observes living cells device in real time.
Background technique
Currently in cell biology, life science and relevant medical research field, cytologic experiment is to frequently refer to
Link.Living cells is life entity fundamental structural unit and functional unit, therefore observes the real-time fluorescence of single living cell to life
Life science is significant, and going deep into quantitative analysis experimental data helps to disclose the information in life science.
In the prior art, the patent of invention that application publication number is CN1802122A discloses a kind of while carrying out real-time multimode
Formula imaging and its spectroscopy use, this measures the optical device of image simultaneously, comprising: light source, for transmitting inquiry broadband spoke
It penetrates；First optical modulator, for separating multiple inquiry spectrum segments that inquiry broadband radiation is corresponding multiple imaging modalities；Target pair
As returning to radiation to generate for image with inquiry spectrum segment reciprocation；Second optical modulator, separation come from target object
Return radiation for corresponding multiple imaging modalities multiple return spectrum segments；At least one detector, which receives, returns to spectrum segment, and
Spectrum segment will be returned to handle as image, it can be achieved that real-time monitoring cell, biological tissue etc..Application publication number is CN109669262A
Patent of invention disclose a kind of electric intelligent fluorescence microscope, the electric intelligent fluorescence microscope, mainly include pedestal, loading
Platform and fluorescing system, pedestal include bottom, limit sliding panel, limit switch, splice plate, small machine, bottom plate, foundation, three mesh head
Seat, Z axis attachment base, Z axis assembly SLDPRT and big motor short axle, the objective table includes short pedestal, object lens adapter, electronic
Nosepiece, electric object lens converter lower contact, camera lens and motorized subject table, the motorized subject table is from a regional observation
With analyze another region, the conversion of object lens multiple from high to low described in the electric object lens converter, electronic Z axis focuses
And remember that Z axis rotates step number, electronic fluorescence automatic switchover fluorescence is photochromic, realizes automatic point of sample data by above step
Function is analysed, and can be realized the function of remotely monitoring under the conditions of biopsy sample.The existing device for observation living cells in real time
Fluorescence microscope, photographic device etc. are set usually in cell incubator, and device is located in incubator, it is easy to incubator
Interior cell generates adverse effect, such as pollutes to cell.
Summary of the invention
Living cells device is observed in real time the object of the present invention is to provide a kind of fluorescence microscope to solve existing real-time sight
Examine the technical issues of living cells device may generate adverse effect to cell.
In order to achieve the goal above, the technical scheme adopted by the invention is as follows: fluorescence microscope observes living cells device in real time,
Including pedestal,
The cell incubator being mounted on the base, the top transparent of cell incubator is so that light passes through；
The observation assembly for the real-time observation cell being mounted on the base；
Observation assembly includes camera lens, support arm, the regulating mechanism for driving support arm to move back and forth, and is equipped with optics in support arm
System, optical system and camera lens cooperate and are connected with control system.
It further, include light source, imaging len, camera unit in the optical system, camera unit and control system
Connection, control system include host and display screen to graphics process, storage and to show.
Further, the cell incubator includes that cabinet and the upper cover for closed box, upper cover are hinged on cabinet
On, upper cover is made of transparent material.
Further, the side opposite with hinged side of the upper cover is equipped with the latch components for locking upper cover, lock
Component includes sealing valve to be sealed to cabinet inner cell.
Further, it is tightly connected between the upper cover and cabinet by the sealing strip of setting.
Further, the regulating mechanism includes the rotating member being set on the base, drives the liter that moves up and down of support arm
The sliding part that drop part, drive support arm move left and right, support arm are mounted on the upper end of lifters, and cunning is fixed in the lower end of lifters
On moving part, sliding part is fixedly connected so as to drive support arm to move back and forth when rotating member rotates with rotating member.
Further, the pedestal is disc, and rotating member is the lantern ring being set on pedestal, and pedestal is equipped with for limiting
Determine the annular spacing groove of lantern ring rotation path, lantern ring is equipped with the sliding block for cooperating with annular spacing groove.
Further, the lifters are the first electric pushrod.
Further, the sliding part is the second electric pushrod being laterally arranged.
Beneficial effects of the present invention:
Fluorescence microscope of the invention observes living cells device in real time, and the top transparent of cell incubator does not need to use
It is arranged inside cell incubator in the observation assembly of observation cell in real time, cell and observation device is separated, it can in real time
Observe living cells state, and will not pair or cell generate adverse effect.
Detailed description of the invention
Fig. 1 is that fluorescence microscope observes living cells apparatus structure schematic diagram in real time in embodiment 1；
Fig. 2 is box structure diagram in embodiment 1.
In figure: 1, pedestal；11, annular spacing groove；2, cell incubator；21, cabinet；22, upper cover；221, sealing strip；3,
Latch components；4, camera lens；5, support arm；6, regulating mechanism；61, lantern ring；611, sliding block；62, the first electric pushrod；63, second
Electric pushrod.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art's every other embodiment obtained belong to what the present invention protected
Range.
In the present invention fluorescence microscope observe in real time living cells device embodiment it is as shown in Figs. 1-2.
Embodiment 1
The fluorescence microscope of the present embodiment observes living cells device, including pedestal 1 in real time, and the shape of pedestal is disc.
Cell incubator 2 is installed, the top transparent of cell incubator is so that light passes through on pedestal 1.Cell incubator 2 is cylinder
Shape, cell incubator include cabinet 21 and the upper cover 22 for closed box, and upper cover 22 is circular upper cover, and upper cover 22 is hinged on
On cabinet, upper cover is made of transparent material.The side opposite with hinged side of upper cover 22 is equipped with the lock group for locking upper cover
Part 3, latch components include sealing valve to be sealed to cabinet inner cell.Pass through the sealing strip of setting between upper cover and cabinet
221 are tightly connected.
The observation assembly of observation cell in real time is also equipped on pedestal 1.Observation assembly includes camera lens 4, support arm 5, drives
The regulating mechanism 6 that support arm moves back and forth.Regulating mechanism 6 include be arranged on pedestal 1 rotating member, drive support arm on move down
Dynamic lifters, the sliding part for driving support arm to move left and right, support arm are mounted on the upper end of lifters, the lower end of lifting key with
Rotating member is fixedly connected so as to drive support arm to move back and forth when rotating member rotates.
Pedestal 1 is disc, and rotating member is the lantern ring 61 being set on pedestal, and pedestal 1 is equipped with to be turned for limiting lantern ring
The annular spacing groove 11 in dynamic path, lantern ring 61 are equipped with the sliding block 611 for cooperating with annular spacing groove, sliding block and annular stop
Slot damping cooperation is so that its support arm can compare to be stably fixed to corresponding position.Lifters are the first electric pushrod
62, the setting of the first electric pushrod up and down direction can drive support arm to move up and down, so that camera lens can move up and down, convenient for adjusting
Camera lens is saved to suitable position, to find optimal observation point.Sliding part is the second electric pushrod 63 being laterally arranged, second
Electric pushrod can drive support arm to move back and forth in the horizontal direction, be convenient for camera adjusting to suitable observation position, the
Two electric pushrods and lantern ring are integrally formed.First electric rod and the second electric rod are equipped with for controlling its control stretched back and forth
Switch.Control switch can control support arm or more, left and right to move back and forth.
Support arm 5 is arcuate structure, and optical system is equipped in support arm, and optical system and camera lens cooperate and is connected with control
System.It include light source, imaging len, camera unit in optical system, camera unit is connect with control system, and control system includes
Host and display screen are to graphics process, storage and to show.Light source is metal-halide light source.Camera unit is miniature takes the photograph
The image information of recording is transferred to the host of control system by camera, microcam.Host has storage and control function,
And the image for showing cell can be cooperated with display screen.
Embodiment 2
Between constant temperature described in the present embodiment, constant humidity plasticizing sample automation control solidification, general configuration and embodiment 1
Unanimously, but be not both with embodiment 1: cell incubator is tetragonal structure.
Embodiment 3
Between constant temperature described in the present embodiment, constant humidity plasticizing sample automation control solidification, general configuration and embodiment 1
Unanimously, but be not both with embodiment 1: a portion of upper cover is made by transparent material, defines this part as observation
Window, observation window is on upper lid.
Embodiment 4
Between constant temperature described in the present embodiment, constant humidity plasticizing sample automation control solidification, general configuration and embodiment 1
Unanimously, but be not both with embodiment 1: sliding part include sliding rail and with sliding rail cooperation pulley, it is luxurious to be hindered between pulley
Buddhist nun cooperates so that pulley can be stably fixed on sliding rail under not by external force.
Embodiment 5
Between constant temperature described in the present embodiment, constant humidity plasticizing sample automation control solidification, general configuration and embodiment 1
Unanimously, but be not both with embodiment 1: support arm is L-type structure.
Claims (9)
1. fluorescence microscope observes living cells device in real time, which is characterized in that including
Pedestal,
The cell incubator being mounted on the base, the top transparent of cell incubator is so that light passes through；
The observation assembly for the real-time observation cell being mounted on the base；
Observation assembly includes camera lens, support arm, the regulating mechanism for driving support arm to move back and forth, and is equipped with optical system in support arm
System, optical system and camera lens cooperate and are connected with control system.
2. fluorescence microscope according to claim 1 observes living cells device in real time, which is characterized in that the optical system
Interior includes light source, imaging len, camera unit, and camera unit is connect with control system, and control system includes host and display
Screen is to graphics process, storage and to show.
3. fluorescence microscope according to claim 1 observes living cells device in real time, which is characterized in that the cell culture
Case includes that cabinet and the upper cover for closed box, upper cover are hinged on cabinet, and upper cover is made of transparent material.
4. fluorescence microscope according to claim 3 observes living cells device in real time, which is characterized in that the upper cover with
The hinged opposite side in side is equipped with the latch components for locking upper cover, latch components include sealing valve with to cabinet inner cell into
Row sealing.
5. fluorescence microscope according to claim 3 observes living cells device in real time, which is characterized in that the upper cover and case
It is tightly connected between body by the sealing strip of setting.
6. fluorescence microscope according to claim 1 observes living cells device in real time, which is characterized in that the regulating mechanism
Including be set on the base rotating member, drive support arm to move up and down lifters, drive the sliding that moves left and right of support arm
Part, support arm are mounted on the upper end of lifters, and the lower end of lifters is fixed on sliding part, and sliding part is fixedly connected with rotating member
So as to drive support arm to move back and forth when rotating member rotates.
7. fluorescence microscope according to claim 6 observes living cells device in real time, which is characterized in that the pedestal is circle
Dish type, rotating member are the lantern ring being set on pedestal, and pedestal is equipped with the annular spacing groove for limiting lantern ring rotation path, set
Ring is equipped with the sliding block for cooperating with annular spacing groove.
8. fluorescence microscope according to claim 6 observes living cells device in real time, which is characterized in that the lifters are
First electric pushrod.
9. fluorescence microscope according to claim 6 observes living cells device in real time, which is characterized in that the sliding part is
The second electric pushrod being laterally arranged.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201910826119.7A CN110487766A (en) | 2019-09-03 | 2019-09-03 | Fluorescence microscope observes living cells device in real time |
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| CN201910826119.7A CN110487766A (en) | 2019-09-03 | 2019-09-03 | Fluorescence microscope observes living cells device in real time |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101012447A (en) * | 2007-02-02 | 2007-08-08 | 杭州浙大优创科技有限公司 | Microscope detection device of direct microscopy method milk somatic cell/bacteria automatic calculating instrument and microscope focusing method |
| CN101048492A (en) * | 2004-11-09 | 2007-10-03 | 株式会社日立医药 | Cell cultivating device, image processing device and cell detecting system |
| CN105331536A (en) * | 2015-11-25 | 2016-02-17 | 浙江大学 | Cell cultivation device applied to microscopic observation |
| CN206872853U (en) * | 2017-06-17 | 2018-01-12 | 王江涵 | Biological cell culture apparatus |
| CN208255525U (en) * | 2018-06-30 | 2018-12-18 | 汤华晓 | A kind of pathology microscope stand |
-
2019
- 2019-09-03 CN CN201910826119.7A patent/CN110487766A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101048492A (en) * | 2004-11-09 | 2007-10-03 | 株式会社日立医药 | Cell cultivating device, image processing device and cell detecting system |
| CN101012447A (en) * | 2007-02-02 | 2007-08-08 | 杭州浙大优创科技有限公司 | Microscope detection device of direct microscopy method milk somatic cell/bacteria automatic calculating instrument and microscope focusing method |
| CN105331536A (en) * | 2015-11-25 | 2016-02-17 | 浙江大学 | Cell cultivation device applied to microscopic observation |
| CN206872853U (en) * | 2017-06-17 | 2018-01-12 | 王江涵 | Biological cell culture apparatus |
| CN208255525U (en) * | 2018-06-30 | 2018-12-18 | 汤华晓 | A kind of pathology microscope stand |
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Application publication date: 20191122 |
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