CN101012447A - Microscope detection device of direct microscopy method milk somatic cell/bacteria automatic calculating instrument and microscope focusing method - Google Patents
Microscope detection device of direct microscopy method milk somatic cell/bacteria automatic calculating instrument and microscope focusing method Download PDFInfo
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- CN101012447A CN101012447A CN 200710067169 CN200710067169A CN101012447A CN 101012447 A CN101012447 A CN 101012447A CN 200710067169 CN200710067169 CN 200710067169 CN 200710067169 A CN200710067169 A CN 200710067169A CN 101012447 A CN101012447 A CN 101012447A
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Abstract
The invention discloses a self-counting microscopic detecting device, which comprises the following parts: microscopic objective lens and digital cam on the draw tube, upper-lower adjusting driving mechanism in the draw tube, driving and transmitting mechanism on the lower end of objective lens and moving out mechanism of glass-carrying piece from transmitting mechanism. The focusing method of microscope comprises the following steps: setting error parameter of brightness gradient value; shooting milk sample image on the glass-carrying piece; adjusting objective lens of microscope; shooting milk sample image on the glass-carrying piece again; comparing brim brightness gradient value of two images; affirming focus; focusing.
Description
Technical field
The invention belongs to dairy products somatic cell/bacteria counting instrument, be specifically related to a kind of with microscopic examination device and focusing of microscope method in the direct microscopy method automatic counter for counting milk somatic cell/bacteria that is ultimate principle.
Background technology
Dairy products somatocyte automatic analyser is the instrument that somatocyte in the dairy products is counted automatically and count results is analyzed automatically.After the milk cattle infected mazoitis, the most obvious and direct symptom is that Ruzhong somatic number (being called for short SCC) increases.Mazoitis not only can cause the minimizing of milk production of cow, and can cause the decline of milk product quality.Therefore,, can detect the quality of cow's milk on the one hand, guarantee the nutritive value of milk product for the detection of Ruzhong SCC; Can monitor the mazoitis infection state of culturing the factory milk cow on the other hand, thereby can take the necessary precaution measure, improve milk production, reduce financial loss.
The dairy products somatocyte analyser of Xiao Shouing mainly contains the Fossomatic that Denmark Foss company produces in the market
TMThree kinds of the Ku Erte cytoanalyzes that the CRP-200 somatic cell counting instrument that 5000 somatic cell counting instruments, Korea S Incyto company produce and U.S. Beckman Coulter Inc. produce.Fossomatic
TM5000 somatic cell counting instruments are ultimate principle with fluorescence streaming counting method, though its detection speed is fast, the repeatable accuracy and the counting precision that detect are relatively poor, and need often to proofread and correct; The CRP-200 somatic cell counting instrument is a ultimate principle with the fluorescent microscope detection method, and repeatable accuracy and counting precision that it detects are higher, but sampling and dyeing action need manual finishing, and the level of automation of instrument is low, and working efficiency is also lower; The Ku Erte cytoanalyze is ultimate principle with Ku Ertefa, and its counting precision is higher, and detection speed is very fast, but it is not the analytical instrument that is specifically designed to the somatocyte counting, thereby repeatable accuracy is lower, and the early-stage preparations process of sample is complicated and chronic.
Directly the microscopy method is as the general international standard detection method of milk somatic cell check, and it is generally used for proofreading and correct the accuracy of somatocyte analyser and other method.But directly the microscopy method only limits to manual operations at present, and also not having both at home and abroad to sell with this method is the somatocyte automatic analyser of ultimate principle, more is not applicable to the microscopic examination device of direct microscopic method milk somatic cell/bacteria automatic counter.Manual operations not only can cause the low of working efficiency, and can produce the operate miss of artificial property inevitably.
Summary of the invention
Technical problem to be solved by this invention provides a kind of microscopic examination device that can be applied to direct microscopic method milk somatic cell/bacteria automatic counter.For this reason, the present invention is by the following technical solutions: it is provided with micro objective and sees through the digital camera head that object lens are taken milk sample on the slide glass, described micro objective and digital camera head are installed on lens barrel, described microscopic examination device is provided with lens barrel up-down adjustment driving mechanism, described microscopic examination device is being provided with transporting mechanism and the driving mechanism thereof of slide glass being delivered to detection zone below the object lens and sending detection zone below the object lens, described microscopic examination device also is provided with slide glass from mechanism that aforementioned transporting mechanism shifts out.Owing to adopt technical scheme of the present invention, the present invention can utilize mechanism delivery slide glass to micro objective, utilize mechanism to carry out focusing of microscope and after hours with the slide glass removal, can be applicable to the job requirement of direct microscopic method milk somatic cell/bacteria automatic counter, under the control of control device, can finish various actions automatically, eliminate the operate miss of artificial property, the detected result that makes automatic counter for counting milk somatic cell/bacteria accurately and reliably.
Another technical problem to be solved of the present invention provides a kind of focusing of microscope method of direct microscopic method milk somatic cell/bacteria automatic counter microscopic examination device.For this reason, the present invention is by the following technical solutions: it is set with the error parameter of brightness step value, and may further comprise the steps:
1), see through micro objective and take milk sampled images on the slide glass,
2), the fine setting micro objective, change the distance between object lens and the slide glass, take the milk sampled images on the slide glass once more,
3), the image border brightness step value of image of twice shooting before and after the contrast, smaller or equal to the brightness step value error parameter of setting, assert that the position of brightness step value maximum is a focal plane position as the brightness step value difference, finish focusing;
As greater than, repeating step " 2) ", and the image border brightness step value of image of twice shooting before and after the contrast once more, up to the image border brightness step value difference of the image of twice shooting in front and back smaller or equal to the brightness step value error parameter of setting, the position of assert brightness step value maximum is a focal plane position, finishes focusing.
Because adopt technique scheme, focusing of microscope method provided by the present invention is simple, focusing accurately, the detected result of guaranteeing automatic counter for counting milk somatic cell/bacteria is accurately and reliably.
Description of drawings
Fig. 1 is the front schematic view of a kind of microscopic examination device embodiment provided by the present invention.
Fig. 2 is the schematic top plan view of Fig. 1.
Fig. 3 is the A-A sectional view of Fig. 1.
Fig. 4 is the schematic flow sheet of focusing work of the present invention.
Embodiment
With reference to accompanying drawing.It is provided with micro objective 1 and sees through the digital camera head 2 that object lens are taken milk sample on the slide glass, described micro objective and digital camera head are installed on lens barrel 3, described microscopic examination device is provided with lens barrel up-down adjustment driving mechanism, described microscopic examination device is being provided with transporting mechanism and the driving mechanism thereof of slide glass 100 being delivered to detection zone below the object lens and sending detection zone below the object lens, described microscopic examination device also is provided with slide glass from mechanism that aforementioned transporting mechanism shifts out.
The control device of the driving mechanism of lens barrel up-down adjustment driving mechanism and aforementioned transporting mechanism comprises computer, micro-chip, photo-sensor or contact switch.
Described lens barrel up-down adjustment driving mechanism comprises stepper-motor 4, the twisted spur gear 5 that is driven by stepper-motor, the tooth bar 6 that cooperates with twisted spur gear, and described lens barrel is connected with tooth bar, makes that the upper and lower displacement of lens barrel is accurate, is convenient to the control of displacement stroke and focusing.Drawing reference numeral 9 excessively moves downward to prevent lens barrel for the photo-sensor of control drive-motor 4.As shown in Figure 2, be connected with swallow-tail form guide groove member 20 on the pillar 18 of microscopic examination device, the lens barrel back side is connected with the dovetail slideway 21 that cooperates with it, and tooth bar 6 is arranged on the dovetail slideway 21.
Described microscopic examination device is being provided with transporting mechanism and the driving mechanism thereof of slide glass being delivered to detection zone below the object lens and sending detection zone below the object lens, transporting mechanism is provided with transfer table 7, transfer table is provided with guiding dovetail-indent 19, described microscopic examination device is provided with base 8, described base is provided with the guide rail 9 that cooperates with the dovetail-indent that leads, and is steadily reliable by making dovetail-indent 19 and cooperating of guide rail 9 the transfer table motion.The driving mechanism of described transporting mechanism comprises drive-motor 10 and is driven the screw rod 11 of driven by motor that transfer table is connected with and screw rod nut engaged 12.Drawing reference numeral 15 is the photo-sensor of control drive-motor 10, and drawing reference numeral 16 is for being connected the catch that cooperates with photo-sensor 15 on the transfer table.Drawing reference numeral 17 is the light source of microscopic examination device.
The invention provides a kind of structure very simply with slide glass from transmitting the mechanism that mechanism shifts out, it comprises and cooperates actuating lever 13 that is moved the platform effect and rotates and the slide glass driving lever 14 that links with actuating lever with transfer table.
During work, after transfer table 7 delivery slide glasss enter microscopic examination Device Testing position, transmitter 15 feeds back signal to micro-chip, micro-chip signals to computer, computer control unit control digital camera head is taken the milk sampled images on the slide glass, control device is controlled lens barrel up-down adjustment driving mechanism again and is driven object lens 1 and digital camera head 2 fine motions and focus and take milk sampled images on the slide glass once more then, whether the edge definition of the image of twice shooting was consistent before and after computer was judged, as inconsistent, computer sends instruction control lens barrel up-down adjustment driving mechanism and drives object lens 1 and digital camera head 2 continuation fine motion focusing, edge definition unanimity until the image of twice shooting in front and back, finish focusing, then control device control drive-motor 10 work, transfer table 7 drives the slide glass fine motion, milk sample on 2 pairs of slide glasss of digital camera head carries out the shooting (being generally 60-90 visual field) of different visual fields, at last, by computer the last photo of taking in each visual field is analyzed counting statistics, to carry out somatocyte or bacterium statistics.
After finishing shooting, 10 work of control device control drive-motor, transfer table moves on, and behind the contact start bar 13, actuating lever 13 is struck and rotates, and drives slide glass driving lever 14 and rotates, and slide glass is transferred to transfer table, skids off instrument by the film trap that goes out that tilts; 10 counter-rotatings of control device control motor drive transfer table and are back to initial bit, accept next piece slide glass.
The focusing of microscope method of direct microscopic method milk somatic cell/bacteria automatic counter microscopic examination device provided by the present invention realizes automatic focusing by the contour edge that detects image.The image outline edge is clear more, and then its brightness step value is just big more, and the contrast value between edge's scenery and the background is just big more in other words.Otherwise, the picture of out of focus, contour edge is smudgy, and brightness step value or contrast gradient descend, and out of focus is far away more, and contrast gradient is low more, utilizes this principle, takes out the big image of brightness step value for clear image, finishes so focus.
With reference to accompanying drawing 4.In computer, set the error parameter of brightness step value earlier; After slide glass entered microscopic examination Device Testing position, transmitter 15 fed back signal to singlechip controller, and micro-chip signals to computer, and computer control digital camera head 2 sees through the milk sampled images on the micro objective shooting slide glass; Control device is controlled motor 4 actions again, and lens barrel up-down adjustment driving mechanism drives object lens 1 and digital camera head 2 fine motions, and the distance between change object lens 1 and the slide glass is focused, and takes the milk sampled images on the slide glass once more.
The edge definition of the image of twice shooting before and after the computer contrast, the image border brightness step value of twice photographic images before and after also promptly contrasting, as the brightness step value error parameter of brightness step value difference smaller or equal to setting, the position of assert brightness step value maximum is a focal plane position, finishes focusing;
As greater than, computer sends instruction, singlechip controller is controlled motor 4 actions again, lens barrel up-down adjustment driving mechanism drive object lens 1 and digital camera head 2 fine motions are focused, and take the milk sampled images on the slide glass once more, repeat, smaller or equal to the brightness step value error parameter of setting, assert that the position of brightness step value maximum is a focal plane position up to the image border of twice photographic images in front and back brightness step value difference, finish focusing.
Claims (5)
1, the microscopic examination device of direct microscopic method milk somatic cell/bacteria automatic counter, it is characterized in that it is provided with micro objective and sees through the digital camera head that object lens are taken milk sample on the slide glass, described micro objective and digital camera head are installed on lens barrel, described microscopic examination device is provided with lens barrel up-down adjustment driving mechanism, described microscopic examination device is being provided with transporting mechanism and the driving mechanism thereof of slide glass being delivered to detection zone below the object lens and sending detection zone below the object lens, described microscopic examination device also is provided with slide glass from mechanism that aforementioned transporting mechanism shifts out.
2, the microscopic examination device of direct microscopic method milk somatic cell/bacteria automatic counter as claimed in claim 1, it is characterized in that the twisted spur gear that described lens barrel up-down adjustment driving mechanism comprises stepper-motor, driven by stepper-motor, the tooth bar that cooperates with twisted spur gear, described lens barrel is connected with tooth bar.
3, the microscopic examination device of direct microscopic method milk somatic cell/bacteria automatic counter as claimed in claim 1, it is characterized in that described transporting mechanism is provided with transfer table, described transfer table is provided with the guiding dovetail-indent, described microscopic examination device is provided with base, described base is provided with the guide rail that cooperates with the dovetail-indent that leads, the driving mechanism of described transporting mechanism comprises drive-motor and is driven the screw rod of driven by motor that transfer table is connected with and the screw rod nut engaged.
4, the microscopic examination device of direct microscopic method milk somatic cell/bacteria automatic counter as claimed in claim 1 is characterized in that described slide glass being comprised and cooperates actuating lever that is moved the platform effect and rotates and the slide glass driving lever that links with actuating lever with transfer table from transmitting mechanism that mechanism shifts out.
5, the focusing of microscope method of direct microscopic method milk somatic cell/bacteria automatic counter microscopic examination device is characterized in that it is set with the error parameter of brightness step value, and may further comprise the steps:
1), see through micro objective and take milk sampled images on the slide glass,
2), the fine setting micro objective, change the distance between object lens and the slide glass, take the milk sampled images on the slide glass once more,
3), the image border brightness step value of image of twice shooting before and after the contrast, smaller or equal to the brightness step value error parameter of setting, assert that the position of brightness step value maximum is a focal plane position as the brightness step value difference, finish focusing;
As greater than, repeating step " 2) ", and the image border brightness step value of image of twice shooting before and after the contrast once more, up to the image border brightness step value difference of the image of twice shooting in front and back smaller or equal to the brightness step value error parameter of setting, the position of assert brightness step value maximum is a focal plane position, finishes focusing.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2007100671699A CN100500861C (en) | 2007-02-02 | 2007-02-02 | Microscope detection device of direct microscopy method for milk somatic cell/bacteria of automatic calculating instrument and microscope focusing method |
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| CNB2007100671699A CN100500861C (en) | 2007-02-02 | 2007-02-02 | Microscope detection device of direct microscopy method for milk somatic cell/bacteria of automatic calculating instrument and microscope focusing method |
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| CN101012447A true CN101012447A (en) | 2007-08-08 |
| CN100500861C CN100500861C (en) | 2009-06-17 |
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102539803A (en) * | 2011-12-30 | 2012-07-04 | 杭州浙大优创科技有限公司 | Detection platform for milk somatic cells |
| CN102660457A (en) * | 2012-04-17 | 2012-09-12 | 南昌航空大学 | Device and method for analyzing and counting blood cells by lensless holographic diffraction imaging |
| CN103031248A (en) * | 2012-12-26 | 2013-04-10 | 綦迎成 | Direct bacterium viewer |
| CN104656223A (en) * | 2015-02-10 | 2015-05-27 | 青岛歌尔声学科技有限公司 | Lens adjusting device |
| CN107111120A (en) * | 2014-12-30 | 2017-08-29 | 格兰富控股联合股份公司 | Method for determining particle |
| CN107607698A (en) * | 2017-10-18 | 2018-01-19 | 深圳乐普智能医疗器械有限公司 | A kind of image collecting device and there is its body fluid cell analyzer |
| CN108760607A (en) * | 2018-07-22 | 2018-11-06 | 湖南乾康科技有限公司 | A kind of microscopic counting pond bracket plate positioning device |
| CN109632609A (en) * | 2018-11-28 | 2019-04-16 | 合肥中元电气技术有限公司 | A kind of fireballing somatic cell counting instrument of counting precision high detection |
| CN110082902A (en) * | 2019-04-09 | 2019-08-02 | 湖南莱博赛医用机器人有限公司 | A kind of glass slide with lens wiping paper and cell analysis equipment |
| CN110487766A (en) * | 2019-09-03 | 2019-11-22 | 河南中医药大学 | Fluorescence microscope observes living cells device in real time |
| CN111044518A (en) * | 2019-12-30 | 2020-04-21 | 长沙协大生物科技有限公司 | Method for efficiently and automatically focusing microscope for vaginal secretion detection |
| CN111413338A (en) * | 2016-08-29 | 2020-07-14 | 爱威科技股份有限公司 | Sample detection system |
| CN112381854A (en) * | 2020-11-13 | 2021-02-19 | 西安闻泰电子科技有限公司 | Image-based motion detection method and device, electronic equipment and storage medium |
| WO2021135393A1 (en) * | 2019-12-31 | 2021-07-08 | 深圳迈瑞生物医疗电子股份有限公司 | Image analysis apparatus and imaging method thereof |
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| CN103592752B (en) * | 2013-11-18 | 2016-05-11 | 广州博隆兴中信息科技有限公司 | A kind of focus control towards live body micro imaging system and focus adjustment method |
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2007
- 2007-02-02 CN CNB2007100671699A patent/CN100500861C/en not_active Expired - Fee Related
Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102539803A (en) * | 2011-12-30 | 2012-07-04 | 杭州浙大优创科技有限公司 | Detection platform for milk somatic cells |
| CN102660457A (en) * | 2012-04-17 | 2012-09-12 | 南昌航空大学 | Device and method for analyzing and counting blood cells by lensless holographic diffraction imaging |
| CN103031248A (en) * | 2012-12-26 | 2013-04-10 | 綦迎成 | Direct bacterium viewer |
| CN107111120A (en) * | 2014-12-30 | 2017-08-29 | 格兰富控股联合股份公司 | Method for determining particle |
| CN104656223A (en) * | 2015-02-10 | 2015-05-27 | 青岛歌尔声学科技有限公司 | Lens adjusting device |
| CN104656223B (en) * | 2015-02-10 | 2017-06-27 | 青岛歌尔声学科技有限公司 | A kind of lens controlling device |
| CN111413338A (en) * | 2016-08-29 | 2020-07-14 | 爱威科技股份有限公司 | Sample detection system |
| CN107607698A (en) * | 2017-10-18 | 2018-01-19 | 深圳乐普智能医疗器械有限公司 | A kind of image collecting device and there is its body fluid cell analyzer |
| CN108760607A (en) * | 2018-07-22 | 2018-11-06 | 湖南乾康科技有限公司 | A kind of microscopic counting pond bracket plate positioning device |
| CN109632609A (en) * | 2018-11-28 | 2019-04-16 | 合肥中元电气技术有限公司 | A kind of fireballing somatic cell counting instrument of counting precision high detection |
| CN110082902A (en) * | 2019-04-09 | 2019-08-02 | 湖南莱博赛医用机器人有限公司 | A kind of glass slide with lens wiping paper and cell analysis equipment |
| CN110082902B (en) * | 2019-04-09 | 2024-04-09 | 湖南莱博赛医用机器人有限公司 | Glass slide with mirror wiping paper and cell analysis equipment |
| CN110487766A (en) * | 2019-09-03 | 2019-11-22 | 河南中医药大学 | Fluorescence microscope observes living cells device in real time |
| CN111044518A (en) * | 2019-12-30 | 2020-04-21 | 长沙协大生物科技有限公司 | Method for efficiently and automatically focusing microscope for vaginal secretion detection |
| WO2021135393A1 (en) * | 2019-12-31 | 2021-07-08 | 深圳迈瑞生物医疗电子股份有限公司 | Image analysis apparatus and imaging method thereof |
| CN112381854A (en) * | 2020-11-13 | 2021-02-19 | 西安闻泰电子科技有限公司 | Image-based motion detection method and device, electronic equipment and storage medium |
| CN112381854B (en) * | 2020-11-13 | 2024-04-19 | 西安闻泰电子科技有限公司 | Image-based motion detection method and device, electronic equipment and storage medium |
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Address after: No. 12, Zhenkang Garden Road, Cambridge, Zhejiang, Hangzhou Patentee after: Hangzhou Ultrasun Technologies Co., Ltd. Address before: 5 floor, building 48, Huang Gu Shan Road, Hangzhou, Zhejiang Patentee before: Hangzhou Ultrasun Technologies Co., Ltd. |
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