CN110470784A - A kind of thin-layer chromatography self-presenting devices and Automatic-expanding method - Google Patents
A kind of thin-layer chromatography self-presenting devices and Automatic-expanding method Download PDFInfo
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- CN110470784A CN110470784A CN201910728806.5A CN201910728806A CN110470784A CN 110470784 A CN110470784 A CN 110470784A CN 201910728806 A CN201910728806 A CN 201910728806A CN 110470784 A CN110470784 A CN 110470784A
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- 238000004809 thin layer chromatography Methods 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title claims abstract description 17
- 239000002904 solvent Substances 0.000 claims abstract description 80
- 238000002474 experimental method Methods 0.000 claims abstract description 20
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 10
- 230000003028 elevating effect Effects 0.000 claims abstract description 7
- 230000006698 induction Effects 0.000 claims description 10
- 239000000523 sample Substances 0.000 claims description 7
- 238000000079 presaturation Methods 0.000 claims description 4
- 238000009434 installation Methods 0.000 claims description 3
- 230000005499 meniscus Effects 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 3
- 230000007423 decrease Effects 0.000 claims 1
- 230000005611 electricity Effects 0.000 claims 1
- 238000012360 testing method Methods 0.000 abstract description 4
- 238000010586 diagram Methods 0.000 description 5
- 238000004587 chromatography analysis Methods 0.000 description 4
- 238000005520 cutting process Methods 0.000 description 4
- 238000011161 development Methods 0.000 description 3
- 238000001514 detection method Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 229910000838 Al alloy Inorganic materials 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 229930013930 alkaloid Natural products 0.000 description 1
- 150000003797 alkaloid derivatives Chemical class 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
- G01N30/94—Development
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
The present invention relates to a kind of thin-layer chromatography self-presenting devices for automatically beginning to and terminating experiment and Automatic-expanding methods.It includes expansion cylinder, it further include having the Automatic-expanding agent set being covered on expansion cylinder, the Automatic-expanding agent set includes cylinder cap, control module, elevating mechanism, clamping device, contact solvent sensor, developing solvent leading-edge sensor and lamellae, and control module is electrically connected with motor so that motor drives lifting of lifting table by motor drive part.Using the above structure and after experimental method, thin-layer chromatography Automatic-expanding experimental provision good leak tightness of the present invention, experiment is automatically begun to and terminated, the influence of external environment and human factor during thin-layer chromatography is reduced, improves the repeatability and reproducibility of device for thin-layer chromatography test, without manually checking the process of device for thin-layer chromatography test, reduce cost of labor, improve the working efficiency of experimenter, the thin-layer developing cylinder of regular size can be compatible with, it is small in size, facilitate popularization.
Description
Technical field
The present invention relates to biological detection technical field of experiment equipment, refer in particular to a kind of thin layer color for automatically beginning to and terminating experiment
Compose self-presenting devices and Automatic-expanding method.
Background technique
Thin-layer chromatography or thin-layer chromatography (thin-layer chromatography), are to be coated on support plate
Support as stationary phase, using suitable solvent as mobile phase, to mixing sample separated, a kind of layer of identification and quantification
Analyse isolation technics.This is a kind of quick separating such as fatty acid, steroids, amino acid, nucleotide, alkaloid and other a variety of objects
The particularly effective chromatography method of matter.
Currently, Development of Thin-Layer Chromatography experiment mostly uses manual hand manipulation, carried out in open thin-layer developing cylinder
Expansion experiment, thin-layer chromatography need people in the process vulnerable to external environment and interference from human factor, also, during the experiment
Work checks device for thin-layer chromatography test process, and conventional efficient is low, poor reproducibility；In view of this, how to design a kind of automatic thin layer color
Spectral expansion equipment come carry out Development of Thin-Layer Chromatography experiment become have technical problem to be solved.
Summary of the invention
The one kind proposed the purpose of the present invention is to the shortcoming of above situation automatically begins to and terminates experiment
Thin-layer chromatography self-presenting devices and Automatic-expanding method.
In order to achieve the above objectives, the technical solution of the present invention is as follows: a kind of thin-layer chromatography self-presenting devices, include expansion
Cylinder further includes having the Automatic-expanding agent set being covered on expansion cylinder, which includes cylinder cap, control
Module, elevating mechanism, clamping device, contact solvent sensor, developing solvent leading-edge sensor and lamellae, cylinder cap lid are set
On the upper opening of expansion cylinder, control module is set on cylinder cap, and elevating mechanism is connected by motor, motor drive part and lifting platform
It connects, motor is mounted on cylinder cap, and lifting column vertically upward is fixed on lifting platform, and lifting column makes after running through cylinder cap through-hole
It obtains lifting platform to be in expansion cylinder cavity, clamping device is connected on lifting platform, and clamping device, which is folded at the top of lamellae, to be made
Lamellae is erect downwards perpendicular to lifting platform, and contact solvent sensor vertical is installed in below lifting platform, and contact solvent passes
The induction end of sensor and the bottom end of lamellae are concordant, and developing solvent leading-edge sensor is vertically fixed on lifting platform by connecting pipe
Lower section, the induction end of developing solvent leading-edge sensor on lamellae solvent expansion frontal line it is corresponding；Control module connects
Touching solvent sensor, developing solvent leading-edge sensor are electrically connected control motor with motor and drive lifting platform by motor drive part
It is elevated to setting experimental site.
The contact solvent sensor is probe, and the induction end of contact solvent sensor is the bottom end of probe.
The connecting pipe is the interval double pipe structure being connected between lifting platform and developing solvent leading-edge sensor.
The top of the lifting column is further fixed on up and down balance system plate, and several locating rods are fixed at the top of valve deck, and lifting is flat
Weighing apparatus plate is actively socketed in several locating rods so that lifting of lifting table is stablized.
The motor is stepper motor.
The clamping device is spring clip or buckle.
The contact solvent sensor and developing solvent leading-edge sensor be infrared sensor or laser sensor or
Person's ultrasonic sensor or visible light sensor.
The control module includes power supply and control chip and motor positive/reverse rotation circuit.
The contact solvent sensor and developing solvent leading-edge sensor are infrared sensor, laser sensor, ultrasound
Wave sensor, visible light sensor.
The sandwiched position at the top of the clamping device and lamellae is additionally provided with pressure sensor, pressure sensor induction
The pressure change data of lamellae arrival cylinder bottom.
Successively include following steps using a kind of Automatic-expanding method of thin-layer chromatography self-presenting devices:
Step 1 starts state, and lamellae is vertically fixed on lifting by clamping device by the preparation and installation of lamellae
The lower section of platform；
Step 2, presaturation state are added solvent in the bottom of expansion cylinder, seal cylinder cap, be arranged by control module
Time started is pre-equilibrated.
Step 3 starts to spread out state, and after arrival time, control module drives lifting platform to link under lamellae by motor
Drop, when lamellae contacts solvent, triggering contact solvent sensor sends a signal to control module stopping motor rotation, from
And carry out expansion experiment；
Step 4, unfolded state, solvent are unfolded along the upward vertical direction of lamellae；
Step 5, solvent front state, when solvent is deployed into developing solvent line forward position, triggering developing solvent forward position is passed
Sensor sends a signal to control module, and control module control motor drives lamellae to be lifted off the experiment of solvent meniscus ends.
In the step 3, when triggering contacts solvent sensor failure, substitute trigger is contacted exhibition by pressure sensor
Open triggering work when agent sensor carries out lamellae contact solvent.
Using the above structure with after experimental method it is found that thin-layer chromatography Automatic-expanding experimental provision good leak tightness of the present invention,
Experiment is automatically begun to and terminated, reduces the influence of external environment and human factor during thin-layer chromatography, improves thin layer
The repeatability and reproducibility of chromatography experiment reduce cost of labor, improve without manually checking the process of device for thin-layer chromatography test
The working efficiency of experimenter, apparatus of the present invention can be compatible with the thin-layer developing cylinder of regular size, small in size, can be used as conventional reality
Equipment use is tested, popularization is facilitated.
Detailed description of the invention
Fig. 1 is the dimensional decomposition structure diagram of the embodiment of the present invention；
Structural schematic diagram when Fig. 2 is the beginning state and presaturation state after cutting of the embodiment of the present invention；
Structural schematic diagram when Fig. 3 is the unfolded state after cutting of the embodiment of the present invention；
Fig. 4 is the structural schematic diagram that solvent front state is deployed into after cutting of the embodiment of the present invention；
Fig. 5 is the structural schematic diagram of end state after cutting of the embodiment of the present invention.
Specific embodiment
1-5 is described in further details a specific embodiment of the invention with reference to the accompanying drawing.
A kind of thin-layer chromatography self-presenting devices of the invention, include expansion cylinder 10, further include being covered on expansion cylinder
Automatic-expanding agent set 20 on 10, the Automatic-expanding agent set include cylinder cap 21, control module 22, elevating mechanism
23, clamping device 24, contact solvent sensor 25, developing solvent leading-edge sensor 26 and lamellae 27, the lid of cylinder cap 21 are set
On the upper opening of expansion cylinder 10, control module 22 be set to cylinder cap 21 on, elevating mechanism 23 by motor, motor drive part and
Lifting platform 231 is formed by connecting, and motor is mounted on cylinder cap 21, and lifting column 232 vertically upward is fixed on lifting platform 231, rises
Drop column 232 is in lifting platform 231 in 10 cavity of expansion cylinder after 21 through-hole of cylinder cap, and clamping device 24 is connected to lifting platform
On 231, the top that clamping device 24 is folded in lamellae 27 erects lamellae 27 downwards perpendicular to lifting platform 231, contacts exhibition
It opens agent sensor 25 and is vertically installed in 231 lower section of lifting platform, contact the induction end of solvent sensor 25 and the bottom of lamellae 27
Hold level with both hands together, developing solvent leading-edge sensor 26 is vertically fixed on the lower section of lifting platform 231, developing solvent by connecting pipe 261
The induction end of leading-edge sensor 26 is corresponding with the solvent expansion frontal line 271 on lamellae 27；Control module 22, contact expansion
Agent sensor 25, developing solvent leading-edge sensor 26 are electrically connected control motor with motor and drive lifting platform by motor drive part
231 are elevated to setting experimental site.
The contact solvent sensor 25 is probe, and the induction end of contact solvent sensor 25 is the bottom end of probe.
The connecting pipe 261 is that the interval that is connected between lifting platform 231 and developing solvent leading-edge sensor 26 is two-tube
Structure.
The top of the lifting column 232 is further fixed on up and down balance system plate 233, and several locating rods are fixed at the top of valve deck 21
211, up and down balance system plate 233 is actively socketed in several locating rods 211 so that the lifting of lifting platform 23 is stablized.
The motor is stepper motor, can adjust height, adjustable distance 0-10cm according to development distance.
The clamping device 24 is spring clip or buckle.
Temperature Humidity Sensor is equipped in 10 cavity of expansion cylinder, which is electrically connected with control module 22；
It is aobvious by control module 22 using the timer record time using temperature and humidity in Temperature Humidity Sensor detection thin-layer developing experiment
Show on a display screen and triggering operates in next step, display screen is LCD display.
The control module 22 includes power supply and control chip and motor positive/reverse rotation circuit；Control chip type can adopt
With ARM chip or STM30 chip or AVR chip or fpga chip or pic chip；Power supply can be used built-in 18650
Size battery or external 12V DC power supply.
The contact solvent sensor 25 and developing solvent leading-edge sensor 26 are infrared sensor or laser sensing
Device or ultrasonic sensor or visible light sensor.
Plastics, kirsite, aluminium alloy, silica gel, stainless steel production can be used in the expansion cylinder 10 and cylinder cap 21.
The clamping device 24 and the sandwiched position at the top of lamellae 27 are additionally provided with pressure sensor, the pressure sensor
Incude the pressure change data that lamellae 27 reaches cylinder bottom.
Successively include following steps using a kind of Automatic-expanding method of thin-layer chromatography self-presenting devices:
Step 1 starts state, and the preparation and installation of lamellae 27 are vertically fixed by clamping device 24 by lamellae 27
In the lower section of lifting platform 231；
Step 2, presaturation state are added solvent 30 in the bottom of expansion cylinder 10, cylinder cap 21 are sealed, by controlling mould
The setting time started of block 22 is pre-equilibrated；
Step 3 starts to spread out state, and after arrival time, control module 22 is linked thin by motor drive lifting platform 231
Laminate 27 declines, and when lamellae 27 contacts solvent 30, triggering contact solvent sensor 25 sends a signal to control module 22
Stop motor rotation, to carry out expansion experiment；
Step 4, unfolded state, solvent 30 are unfolded along the upward vertical direction of lamellae 27；
Step 5, solvent front state trigger developing solvent when solvent 30 is deployed into developing solvent frontal line 271
Leading-edge sensor 26 issues buzzing and alarms and be simultaneously emitted by signal to control module 22, and control module 22 controls motor and drives thin layer
Plate 27 is lifted off the experiment of 30 meniscus ends of solvent.
In the step 3, when triggering contacts 25 failure of solvent sensor, pressure sensor contacts substitute trigger
Solvent sensor 25 carries out triggering work when lamellae 27 contacts solvent 30.
It composes finally, being analysed with thin layer chromatography scanning, is radiated on the chromatoplate after the chromatography of lamellae 27 with the light of certain wavelength,
To having the chromatography spot that absorbs or can generate fluorescence to be scanned, the intensity absorbed is measured with bounce technique or transmission beam method, with inspection
Survey chromatogram.
Certainly, the present invention can also have other numerous embodiments, ripe without departing substantially from spirit of that invention and essence
Various corresponding changes and modifications, but these corresponding changes and modifications ought can be made according to invention by knowing those skilled in the art
It all should belong to the protection scope of invention the attached claims.
Claims (10)
1. a kind of thin-layer chromatography self-presenting devices, include expansion cylinder, it is characterised in that: further include being covered on expansion cylinder
Automatic-expanding agent set, which includes cylinder cap, control module, elevating mechanism, clamping device, connects
Touching solvent sensor, developing solvent leading-edge sensor and lamellae, cylinder cap lid are located on the upper opening of expansion cylinder, are controlled
Module is set on cylinder cap, and elevating mechanism is formed by connecting by motor, motor drive part and lifting platform, and motor is mounted on cylinder cap,
Lifting column vertically upward is fixed on lifting platform, lifting column makes lifting platform be in expansion cylinder cavity after running through cylinder cap through-hole
Interior, clamping device is connected on lifting platform, and clamping device, which is folded at the top of lamellae, keeps lamellae downward perpendicular to lifting platform
It erects, contact solvent sensor vertical is installed in below lifting platform, contacts the induction end and lamellae of solvent sensor
Bottom end is concordant, and developing solvent leading-edge sensor is vertically fixed on the lower section of lifting platform by connecting pipe, and developing solvent forward position passes
The induction end of sensor is corresponding with the solvent expansion frontal line on lamellae；Control module, contact solvent sensor, expansion are molten
Agent leading-edge sensor is electrically connected control motor with motor and drives lifting of lifting table to setting experimental site by motor drive part.
2. a kind of thin-layer chromatography self-presenting devices according to claim 1, it is characterised in that: the contact solvent passes
Sensor is probe, and the induction end of contact solvent sensor is the bottom end of probe.
3. a kind of thin-layer chromatography self-presenting devices according to claim 2, it is characterised in that: the connecting pipe is to connect
Connect the interval double pipe structure between lifting platform and developing solvent leading-edge sensor.
4. a kind of thin-layer chromatography self-presenting devices according to claim 3, it is characterised in that: the top of the lifting column
It is further fixed on up and down balance system plate, is fixed with several locating rods at the top of valve deck, up and down balance system plate is actively socketed on several positioning
So that lifting of lifting table is stablized on bar.
5. a kind of thin-layer chromatography self-presenting devices according to claim 4, it is characterised in that: the motor is stepping electricity
Machine；The clamping device is spring clip or buckle.
6. a kind of thin-layer chromatography self-presenting devices according to claim 5, it is characterised in that: in the expansion cylinder cavity
Equipped with Temperature Humidity Sensor, which is electrically connected with control module；The control module includes power supply and control
Chip and motor positive/reverse rotation circuit.
7. a kind of thin-layer chromatography self-presenting devices according to claim 6, it is characterised in that: the contact solvent passes
Sensor and developing solvent leading-edge sensor are infrared sensor or laser sensor or ultrasonic sensor or visible light
Sensor.
8. a kind of thin-layer chromatography self-presenting devices according to claim 1, it is characterised in that: the clamping device with it is thin
The sandwiched position at the top of laminate is additionally provided with pressure sensor, which incudes the pressure change that lamellae reaches cylinder bottom
Data.
9. use a kind of Automatic-expanding method of thin-layer chromatography self-presenting devices according to any one of claims 8, successively include with
Lower step:
Step 1 starts state, and lamellae is vertically fixed on lifting platform by clamping device by the preparation and installation of lamellae
Lower section；
Step 2, presaturation state are added solvent in the bottom of expansion cylinder, seal cylinder cap, started by control module setting
Time is pre-equilibrated；
Step 3 starts to spread out state, and after arrival time, control module drives lifting platform linkage lamellae to decline by motor,
When lamellae contacts solvent, triggering contact solvent sensor sends a signal to control module stopping motor rotation, thus into
Row expansion experiment；
Step 4, unfolded state, solvent are unfolded along the upward vertical direction of lamellae；
Step 5, solvent front state trigger developing solvent leading-edge sensor when solvent is deployed into developing solvent frontal line
Control module is sent a signal to, control module control motor drives lamellae to be lifted off the experiment of solvent meniscus ends.
10. a kind of Automatic-expanding method of thin-layer chromatography self-presenting devices according to claim 9, it is characterised in that:
In the step 3, when triggering contacts solvent sensor failure, pressure sensor senses substitute trigger contact solvent
Device carries out triggering work when lamellae contact solvent.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2019105098013 | 2019-06-13 | ||
| CN201910509801 | 2019-06-13 |
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| CN110470784A true CN110470784A (en) | 2019-11-19 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111366674A (en) * | 2020-04-13 | 2020-07-03 | 杭州旭昱科技有限公司 | Multi-oil-cylinder pressurizing device for OPLC (optical phase separation liquid chromatography) pressurized thin-layer chromatograph |
| WO2022101375A1 (en) * | 2020-11-16 | 2022-05-19 | Merck Patent Gmbh | Cover for a thin-layer chromatography development chamber |
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| CN109580867A (en) * | 2018-12-07 | 2019-04-05 | 青海省药品检验检测院 | A kind of Development of Thin-Layer Chromatography instrument |
| CN210803383U (en) * | 2019-06-13 | 2020-06-19 | 广州科曼生物科技有限公司 | Automatic thin-layer chromatography unfolding device |
-
2019
- 2019-08-08 CN CN201910728806.5A patent/CN110470784A/en active Pending
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|---|---|---|---|---|
| GB1428545A (en) * | 1972-10-19 | 1976-03-17 | Takeshita Y | Apparatus for thin-layer or paper chromatography |
| CN2082416U (en) * | 1990-10-23 | 1991-08-07 | 徐敏 | Automatic controller of constant temperature thin-layer development |
| CN201796019U (en) * | 2010-05-31 | 2011-04-13 | 徐金保 | Controlled thin layer chromatograph |
| CN104704357A (en) * | 2012-07-30 | 2015-06-10 | 加利福尼亚大学董事会 | Biomolecular detection test strip design |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111366674A (en) * | 2020-04-13 | 2020-07-03 | 杭州旭昱科技有限公司 | Multi-oil-cylinder pressurizing device for OPLC (optical phase separation liquid chromatography) pressurized thin-layer chromatograph |
| WO2022101375A1 (en) * | 2020-11-16 | 2022-05-19 | Merck Patent Gmbh | Cover for a thin-layer chromatography development chamber |
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