CN110470663A - For quickly detecting the reagent and kit of people's stomach Helicobacter pylori - Google Patents
For quickly detecting the reagent and kit of people's stomach Helicobacter pylori Download PDFInfo
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- CN110470663A CN110470663A CN201910771542.1A CN201910771542A CN110470663A CN 110470663 A CN110470663 A CN 110470663A CN 201910771542 A CN201910771542 A CN 201910771542A CN 110470663 A CN110470663 A CN 110470663A
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- detection reagent
- metallic nickel
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- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 46
- 241000590002 Helicobacter pylori Species 0.000 title claims abstract description 20
- 229940037467 helicobacter pylori Drugs 0.000 title claims abstract description 19
- 210000002784 stomach Anatomy 0.000 title claims abstract description 15
- 238000001514 detection method Methods 0.000 claims abstract description 53
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 claims abstract description 38
- 238000012360 testing method Methods 0.000 claims abstract description 27
- 239000004202 carbamide Substances 0.000 claims abstract description 20
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims abstract description 19
- 239000002904 solvent Substances 0.000 claims abstract description 11
- 238000000034 method Methods 0.000 claims description 24
- 210000003296 saliva Anatomy 0.000 claims description 20
- 239000008187 granular material Substances 0.000 claims description 9
- 230000035484 reaction time Effects 0.000 claims description 7
- 238000007865 diluting Methods 0.000 claims description 2
- 108010046334 Urease Proteins 0.000 description 11
- 238000006243 chemical reaction Methods 0.000 description 11
- 239000000243 solution Substances 0.000 description 9
- 238000006555 catalytic reaction Methods 0.000 description 6
- 229910052759 nickel Inorganic materials 0.000 description 6
- KJFMBFZCATUALV-UHFFFAOYSA-N phenolphthalein Chemical group C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2C(=O)O1 KJFMBFZCATUALV-UHFFFAOYSA-N 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 5
- 235000019441 ethanol Nutrition 0.000 description 5
- 238000007689 inspection Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 208000015181 infectious disease Diseases 0.000 description 4
- 239000002184 metal Substances 0.000 description 4
- 229910052751 metal Inorganic materials 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- DKGAVHZHDRPRBM-UHFFFAOYSA-N Tert-Butanol Chemical compound CC(C)(C)O DKGAVHZHDRPRBM-UHFFFAOYSA-N 0.000 description 3
- 239000012752 auxiliary agent Substances 0.000 description 3
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000002086 nanomaterial Substances 0.000 description 3
- 239000002105 nanoparticle Substances 0.000 description 3
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 2
- 150000001298 alcohols Chemical group 0.000 description 2
- 239000003638 chemical reducing agent Substances 0.000 description 2
- 239000000084 colloidal system Substances 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 239000010931 gold Substances 0.000 description 2
- 229910052737 gold Inorganic materials 0.000 description 2
- ZXEKIIBDNHEJCQ-UHFFFAOYSA-N isobutanol Chemical compound CC(C)CO ZXEKIIBDNHEJCQ-UHFFFAOYSA-N 0.000 description 2
- 239000007791 liquid phase Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 229960003531 phenolsulfonphthalein Drugs 0.000 description 2
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 2
- NWZSZGALRFJKBT-KNIFDHDWSA-N (2s)-2,6-diaminohexanoic acid;(2s)-2-hydroxybutanedioic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O.NCCCC[C@H](N)C(O)=O NWZSZGALRFJKBT-KNIFDHDWSA-N 0.000 description 1
- 206010060976 Bacillus infection Diseases 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 208000007882 Gastritis Diseases 0.000 description 1
- 229910021586 Nickel(II) chloride Inorganic materials 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 208000005718 Stomach Neoplasms Diseases 0.000 description 1
- 208000007107 Stomach Ulcer Diseases 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 239000003146 anticoagulant agent Substances 0.000 description 1
- 229940127219 anticoagulant drug Drugs 0.000 description 1
- 239000002518 antifoaming agent Substances 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- -1 can enumerate Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 208000023652 chronic gastritis Diseases 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000002270 dispersing agent Substances 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 206010017758 gastric cancer Diseases 0.000 description 1
- 210000001156 gastric mucosa Anatomy 0.000 description 1
- 201000005917 gastric ulcer Diseases 0.000 description 1
- 238000000713 high-energy ball milling Methods 0.000 description 1
- IKDUDTNKRLTJSI-UHFFFAOYSA-N hydrazine monohydrate Substances O.NN IKDUDTNKRLTJSI-UHFFFAOYSA-N 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 238000002356 laser light scattering Methods 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- QMMRZOWCJAIUJA-UHFFFAOYSA-L nickel dichloride Chemical compound Cl[Ni]Cl QMMRZOWCJAIUJA-UHFFFAOYSA-L 0.000 description 1
- LGQLOGILCSXPEA-UHFFFAOYSA-L nickel sulfate Chemical compound [Ni+2].[O-]S([O-])(=O)=O LGQLOGILCSXPEA-UHFFFAOYSA-L 0.000 description 1
- 229910000363 nickel(II) sulfate Inorganic materials 0.000 description 1
- BFDHFSHZJLFAMC-UHFFFAOYSA-L nickel(ii) hydroxide Chemical compound [OH-].[OH-].[Ni+2] BFDHFSHZJLFAMC-UHFFFAOYSA-L 0.000 description 1
- 229910000069 nitrogen hydride Inorganic materials 0.000 description 1
- 239000003002 pH adjusting agent Substances 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 210000001187 pylorus Anatomy 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000036632 reaction speed Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 201000011549 stomach cancer Diseases 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 238000012956 testing procedure Methods 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 230000000472 traumatic effect Effects 0.000 description 1
- 230000002485 urinary effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N2021/775—Indicator and selective membrane
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plasma & Fusion (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Provide a kind of detection reagent, including urea, metallic nickel and necessary solvent.In addition, additionally providing the kit for detecting people's stomach Helicobacter pylori, including sampler, reactor and test paper；The reactor accommodates aforementioned detection reagent.The reagent and kit can realize the quick detection of people's stomach Helicobacter pylori, and accuracy and specificity are high, and detection time is short.
Description
Technical field
The invention belongs to technical field of biological；It is related to a kind of for quickly detecting the reagent of people's stomach Helicobacter pylori
And kit.
Background technique
Stomach Helicobacter pylori (abbreviation HP) is the helical form gramnegative bacterium in a kind of human body gastric mucosa, it is to lead
Cause the main pathogenic of chronic gastritis, gastric ulcer, even gastric cancer.Epidemiological study shows China and most of development China
Helicobacter pylori positive rate in family rises with age, and the 20-40 years old infection rate in China is 45.4%-
63.6%, 70 years old or more infection rate is up to 78.9%, and children's positive rate about after 10 years old is about 10%~20%, because
Fast and effectively HP detection mode has extremely important and real clinical meaning for this development.
HP infection clinical testing procedure be broadly divided into it is invasive with two kinds of Noninvasive.Invasive detection method has centainly
It is traumatic, coagulation function is poor, take anticoagulant patient or infant compliance it is poor；And Noninvasive detection method wound
Property it is smaller, compensate for it is invasive detection compliance difference deficiency；Have the characteristics that cheap, easy to be quick simultaneously, receives
The extensive concern of developer.Common Noninvasive detection method includes13C and14C- urea breath test (UBT),15N- urinary ammonia
Discharge test (UDT), the colloidal gold method based on antibody-antigen immune reaction principle, the fast urease method of inspection and molecule are raw
Object method.
Wherein, not only reaction speed is very fast for the fast urease method of inspection, but also expense is lower.The principle of this method
Be: the urease that helicobacter pylori generates obtains product CO after decomposing2And NH3, the latter will lead to pH value raising, to increase stomach
The alkalinity of mucous membrane tissue, therefore can be judged whether the infection by helicobacter pylori by the color change of pH value.
Shandong University Guo supports the army et al. discloses a kind of qualitative detection pylorus spiral shell in Chinese patent application CN1916608A
The reaction box of bacillus infection, including box body and it is located at that box is intracorporal using reagent, and there are plastic reaction plate and the China of scrobicula in surface
Special door test paper；The test paper is to be impregnated with the test paper dried again completely through 1% urea liquid and phenolic red indicator, is to steam using reagent
Distilled water and 1% urea enzyme solutions.This method raw material sources are abundant, cheap；It is fast to detect speed, and qualitative accurate.So
And the test paper fails after long-time room temperature preservation since urea decomposes, while the reaction box is under cryogenic (15 DEG C)
Under accuracy substantially reduce.
Li Xiong et al. discloses a kind of saliva in Chinese patent application CN104263812A and quickly detects helicobacter pylori
Method and its kit, method includes the following steps: collect saliva 0.5-1ml, after drawing out 0.2 ± 0.02ml saliva,
Remaining salivary pH is detected with pH test paper and is recorded；It reacts 5-20 minutes, uses after the saliva of suction is sufficiently mixed with detection liquid
The pH value and record of pH test paper detection reaction solution；By the pH value of reaction solution compared with salivary pH, if the pH value of reaction solution is big
When salivary pH, reaction result is considered as the positive, that is, contains urease, and there are helicobacter pyloris；If reaction solution
PH value be lower than salivary pH when then reaction result be considered as feminine gender, that is, be free of urease, be not present helicobacter pylori.This method
Sensitivity, specificity, positive predictive value and negative predictive value with14C-UBT is close, the two testing result no statistical difference meaning
Justice.However, the pH value measurement error of the detection method is larger, while activity is lower, and detection time is longer.
In short, the above-mentioned fast urease method of inspection there is accuracys to varying degrees not high, detection time compared with
The long and not high technological deficiency of specificity.Therefore in view of the above-mentioned drawbacks in the prior art, a kind of reliable there is an urgent need to find
For quickly detect people's stomach Helicobacter pylori reagent and kit.
Summary of the invention
An object of the present invention is to provide a kind of new reagent for quickly detection people's stomach Helicobacter pylori, the examination
The quick detection of people's stomach Helicobacter pylori can be achieved in agent, and accuracy and specificity are high, and detection time is short.
The second object of the present invention is to provide a kind of reagent that people's stomach Helicobacter pylori is quickly detected using mentioned reagent
Box.
Inventors have found that reagent and kit of the invention utilize the catalysis technique of novel metal nickel nano particle, pass through
Nano material high-ratio surface, high catalysis characteristics test the detection to urease in saliva rapidly and sensitively, to judge depositing for HP
In.The kit is easy to operate, it is easy, reliable, repeat, be widely used, is at low cost, is safe to examiner, free from environmental pollution,
The shortcomings that overcoming background technique.
Based on above-mentioned mentality of designing, present invention employs following technical solutions: a kind of detection reagent, including urea, metal
Nickel and necessary solvent.
Detection reagent above-mentioned according to the present invention, wherein the metallic nickel is metallic nickel nano granule.
Detection reagent above-mentioned according to the present invention, wherein the average grain diameter of the metallic nickel nano granule is 10-100nm.
In the present invention, average grain diameter indicates the D50 value of laser light scattering method measurement.
Advantageously, the average grain diameter of the metallic nickel nano granule is 10-80nm；It is preferred that 10-60nm, more preferable 10-
50nm, and most preferably 10-40nm.
In a specific embodiment, the average grain diameter of the metallic nickel nano granule is 28nm.
Detection reagent above-mentioned according to the present invention, wherein the preparation method of the metallic nickel nano granule is this field skill
Known to art personnel, include, but are not limited to: plasma method, liquid phase reduction, spark discharge etch and high-energy ball milling
Method.
In a specific embodiment, the preparation method of the metallic nickel nano granule is liquid phase reduction, by closing
Suitable nickel source is restored under reducing agent effect and is obtained.
As nickel source, such as nickel chloride, nickel sulfate and nickel hydroxide can be enumerated, etc..As reducing agent, such as can enumerate
Hydrazine hydrate.
Detection reagent above-mentioned according to the present invention, wherein the dosage of the urea is 0.01-1g/mL.
Preferably, the dosage of the urea is 0.02-0.8g/mL；It is highly preferred that the dosage of the urea is 0.05-
0.5g/mL；And most preferably, the dosage of the urea is 0.1-0.3g/mL.
In a specific embodiment, the dosage of the urea is 0.2g/mL.
Detection reagent above-mentioned according to the present invention, wherein the dosage of the metallic nickel is 0.01-1mg/mL.
Preferably, the dosage of the metallic nickel is 0.02-0.8mg/mL；It is highly preferred that the dosage of the metallic nickel is
0.04-0.5mg/mL；And most preferably, the dosage of the metallic nickel is 0.05-0.2mg/mL.
In a specific embodiment, the dosage of the metallic nickel is 0.1mg/mL.
Detection reagent above-mentioned according to the present invention, wherein solvent is selected from alcohols solvent.
As alcohols solvent, such as methanol, ethyl alcohol, propyl alcohol, isopropanol, butanol, isobutanol, the tert-butyl alcohol, second two can be enumerated
Alcohol, propylene glycol, etc..
Detection reagent above-mentioned according to the present invention, wherein further comprise auxiliary agent.
It as auxiliary agent, such as can enumerate, dispersing agent, wetting agent, thickener, defoaming agent, surfactant, pH adjusting agent,
Etc..
In a specific embodiment, the auxiliary agent is polyvinylpyrrolidone.
On the other hand, the present invention also provides a kind of methods for preparing aforementioned detection reagent according to the present invention, comprising: by institute
It states metallic nickel and urea is separately or concurrently dissolved or dispersed in solvent, obtain detection reagent above-mentioned according to the present invention.
In a specific embodiment, the metallic nickel is first dissolved or dispersed in solvent, adds urea, obtained
To detection reagent above-mentioned according to the present invention.
In another particular embodiment of the invention, urea is first dissolved or dispersed in solvent, adds the metallic nickel,
Obtain detection reagent above-mentioned according to the present invention.
In another specific embodiment, the metallic nickel and urea are dissolved or dispersed in solvent simultaneously, obtained
To detection reagent above-mentioned according to the present invention.
Another aspect, the present invention also provides a kind of for detecting the kit of people's stomach Helicobacter pylori, including sampling
Device, reactor and test paper；It is characterized in that, the reactor accommodates detection reagent above-mentioned according to the present invention.
Kit above-mentioned according to the present invention, wherein the sampler is used to that saliva sample to be added, the saliva sample
Dosage is 0.05-10.0mL.
Preferably, the dosage of the saliva sample is 0.1-8.0mL；It is highly preferred that the dosage of the saliva sample is
0.2-5.0mL；And most preferably, it is preferable that the dosage of the saliva sample is 0.5-2.0mL.
Kit above-mentioned according to the present invention, wherein sampler is added after diluting certain multiple in the saliva sample.
Preferably, extension rate is 20-2000 times；It is highly preferred that extension rate is 50-1500 times；And most preferably
Ground, extension rate are 100-1000 times.
In a specific embodiment, extension rate is 200 times.
Kit above-mentioned according to the present invention, wherein the reaction time of the reactor is 0.1-5min.
Preferably, the reaction time of the reactor is 0.2-4min；It is highly preferred that the reaction time of the reactor is
0.5-3min；And most preferably, the reaction time of the reactor is 1-2min.
In a specific embodiment, the reaction time of the reactor is 1.5min.
Kit above-mentioned according to the present invention, wherein the test paper is phenolphthalein or phenol red test paper.
As phenolphthalein or phenol red test paper, preparation method is well known to those skilled in the art.
In a specific embodiment, dry test paper is punched, is prepared into the disk of round 0.6-1cm, impregnated
In configured phenolphthalein or phenol red solution, then it is dried to obtain.
Compared with prior art, reagent of the invention and kit utilize the catalysis technique of novel metal nickel nano particle,
By nano material high-ratio surface, high catalysis characteristics test the detection to urease in saliva rapidly and sensitively, to judge HP
Presence.The reagent and kit can realize the quick detection of people's stomach Helicobacter pylori, and accuracy and specificity are high, inspection
It is short to survey the time.
Detailed description of the invention
Fig. 1 is the primary structure schematic diagram of kit of the present invention；Wherein, 1- result observation area；2- test paper；3- sampler；
4- detection reagent；5- reactor；6- samples graduation mark；7- top cover switch；8- top cover.
Specific embodiment
Illustrate reagent and kit of the invention below with reference to specific embodiment.
It should be noted that a specific embodiment of the invention is only used for illustrating the spirit and principles in the present invention, rather than limit
The scope of the present invention processed.In addition, it should also be understood that, after reading the contents of the present invention, those skilled in the art can be to the present invention
Technical solution make it is various change, replace, delete, correct or adjust, these equivalent technical solutions equally fall within power of the present invention
Sharp claim limited range.
Embodiment 1 prepares detection reagent
The high stability of monodispersity and the metallic nickel nano granule of high activity are prepared first, and average grain diameter D50 is 28nm；
Then 0.05g is taken to prepare metallic nickel ethanol solution/dispersion liquid of 0.1mg/mL；Urea is then added, obtaining concentration is 0.2g/mL
The mixed solution of the nano nickel of urea and 0.1mg/mL.
2 reagent preparation box of embodiment
As shown in Figure 1, the kit primary structure of embodiment 2 includes: 1- result observation area；2- test paper；3- sampler；4-
Detection reagent；5- reactor；6- samples graduation mark；7- top cover switch；8- top cover.
Wherein, detection reagent of the detection reagent from embodiment 1.
The about diluted saliva sample of certain volume is added using sampler, until scale in sample cell, covers tightly lid, reaction
After a certain period of time, it overturns, test paper color change is observed by observation area, if there is color change (becoming light red or aubergine),
It is then positive reaction.
Effect test
It is detected respectively using different samples, testing result is as shown in table 1 below.
Table 1
| Sample | Saliva measures (ml) | Reaction time (min) | Accuracy |
| 1 | 0.1ml | 0.5 | + |
| 2 | 0.1ml | 1 | ++ |
| 3 | 0.1ml | 2 | ++ |
| 4 | 0.2ml | 0.5 | + |
| 5 | 0.2ml | 1 | ++ |
| 6 | 0.2ml | 2 | ++ |
| 7 | 0.5ml | 0.5 | ++ |
| 8 | 0.5ml | 1 | ++ |
| 9 | 0.5ml | 2 | +++ |
| 10 | 1.0ml | 0.5 | +++ |
| 11 | 1.0ml | 1 | +++ |
| 12 | 1.0ml | 2 | +++ |
Wherein, +++ indicate different samples using detection method repeated detection result accuracy 85-95% it
Between；++ it indicates between 75-85%；+ indicate between 65-75%.
Comparative test
Detection method of the invention and urease saliva method and colloid gold test paper method are compared, in different extension rates
Under accuracy it is as shown in table 2 below.
Table 2
| Extension rate | The present invention | Urease saliva method | Colloid gold test paper method |
| 1 | + | - | - |
| 1*101 | ++ | + | - |
| 1*102 | +++ | + | - |
| 1*103 | +++ | ++ | + |
Wherein ,-indicate to be lower than 65%.
Reagent of the invention and kit utilize the catalysis technique of novel metal nickel nano particle it can be seen from table 1-2,
By nano material high-ratio surface, high catalysis characteristics test the detection to urease in saliva rapidly and sensitively, to judge HP
Presence.The reagent and kit can realize the quick detection of people's stomach Helicobacter pylori, and accuracy and specificity are high, inspection
It is short to survey the time.
These are only the preferred embodiment of the present invention, is not intended to restrict the invention, for those skilled in the art
For member, the invention may be variously modified and varied.All within the spirits and principles of the present invention, it is made it is any modification,
Equivalent replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of detection reagent, including urea, metallic nickel and necessary solvent.
2. detection reagent according to claim 1, wherein the metallic nickel is metallic nickel nano granule.
3. detection reagent according to claim 2, wherein the average grain diameter of the metallic nickel nano granule is 10-
100nm。
4. detection reagent according to claim 1, wherein the dosage of the urea is 0.01-1g/mL.
5. detection reagent according to claim 1, wherein the dosage of the metallic nickel is 0.01-1mg/mL.
6. a kind of method for preparing any one of -5 detection reagents according to claim 1, comprising: by the metallic nickel and urea
It is separately or concurrently dissolved or dispersed in solvent, obtains detection reagent according to claim 1-5.
7. a kind of for detecting the kit of people's stomach Helicobacter pylori, including sampler, reactor and test paper；Its feature exists
In the reactor accommodates detection reagent according to claim 1.
8. kit according to claim 7, wherein the sampler is for being added saliva sample, the saliva sample
Dosage be 0.05-10.0mL.
9. kit according to claim 9, wherein sampler is added after diluting certain multiple in the saliva sample；
Preferably, extension rate is 20-2000 times.
10. kit according to claim 9, wherein the reaction time of the reactor is 0.1-5min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910771542.1A CN110470663B (en) | 2019-08-21 | 2019-08-21 | Reagent and kit for rapidly detecting human gastric helicobacter pylori |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910771542.1A CN110470663B (en) | 2019-08-21 | 2019-08-21 | Reagent and kit for rapidly detecting human gastric helicobacter pylori |
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| Publication Number | Publication Date |
|---|---|
| CN110470663A true CN110470663A (en) | 2019-11-19 |
| CN110470663B CN110470663B (en) | 2020-10-30 |
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| CN201910771542.1A Active CN110470663B (en) | 2019-08-21 | 2019-08-21 | Reagent and kit for rapidly detecting human gastric helicobacter pylori |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN114621995A (en) * | 2022-05-16 | 2022-06-14 | 广州贝思奇诊断试剂有限公司 | Test paper for rapidly detecting gastric helicobacter pylori by using collected tartar as well as preparation method and application of test paper |
| CN114621995B (en) * | 2022-05-16 | 2022-08-19 | 广州贝思奇诊断试剂有限公司 | Test paper for rapidly detecting helicobacter pylori in stomach by using collected tartar as well as preparation method and application of test paper |
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