CN1916608A - Reaction box for quick qualitative detecting infection of helicobacter pylori, and application - Google Patents
Reaction box for quick qualitative detecting infection of helicobacter pylori, and application Download PDFInfo
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- CN1916608A CN1916608A CN 200610068763 CN200610068763A CN1916608A CN 1916608 A CN1916608 A CN 1916608A CN 200610068763 CN200610068763 CN 200610068763 CN 200610068763 A CN200610068763 A CN 200610068763A CN 1916608 A CN1916608 A CN 1916608A
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- helicobacter pylori
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Abstract
A kit for quickly determining infection of polyloric spirillum consists of kit body, application reagent prepared from distilled water and 1% of urase solution, plastic reaction plate with round scrobicula on its surface and Huatemen test paper prepared by soaking said paper completely in 1% of urea solution and in phenol red indicator then drying it in the air.
Description
Technical field
The present invention relates to a kind of reaction box and application of diagnosing peptic ulcer, relate in particular to a kind of reaction box and application of helicobacter pylori infections in the diagnosis peptic ulcer of qualitative detecting infection of helicobacter pylori.
Background technology
Helicobacter pylori (Helicobacter pylori, HP) infect be chronic Type B gastritis main diseases because of, in close relations with non-ulcer type indigestion, gastric duodenal ulcer and cancer of the stomach.Epidemiological study shows, the HP infection rate is 39.9～49.6% in the asymptomatic or healthy population of compatriots.
Traditional helicobacter pylori detection method mainly comprises: the one, directly from gastric mucosa, detect HP, comprise microbe growth, tissue smear and section statining, the 2nd, urease test or
14C expiration laboratory diagnosis HP infects, and the 3rd, immunological method such as ELISA etc. have methods such as serology detection and PCR in recent years again.The goldstandard that current diagnosis HP infects is pathology of gastric mucosa tissue examination and microbe growth, but takes time and effort, and spends higherly, is unfavorable for clinical practice.Be not suitable for mass detection, make troubles for the detection of HP.The RUT diagnosis has very high susceptibility and specificity, is respectively 85% and 90%, and positive prediction rate and negative prediction rate are respectively 94% and 76% (Mumtaz, Khalid; Abid, Shahab; Yakoob, Javed; Abbas, Zaigham; Hamid, Saeed; Islam, M.; Shah, Hasnain A.; Jafri, Wasim.An office-based serological test for detection of current Helicobacter pyloriinfection:is it useful? European Journal of Gastroenterology ﹠amp; Hepatology.18 (1): 85-88, January 2006.), be widely used in clinical.
The dried test paper method of state-of-the art Pronto dry is the most responsive at present the most dried paper detection method, but spends higher.Therefore measuring the HP infection how fast, accurately, economically is one of clinical problem that faces.
Summary of the invention
At the deficiencies in the prior art, the object of the present invention is to provide a kind of reaction box of quick qualitative detecting infection of helicobacter pylori.This reaction box is not only drawn materials easy to make, and the more important thing is and can carry out qualitative detection to helicobacter pylori infections apace, and accurate, economical.
Another object of the present invention provides the application of reaction box in carrying out qualitative detecting infection of helicobacter pylori of this qualitative detecting infection of helicobacter pylori.
State in realization in the goal of the invention, the reaction box of quick qualitative detecting infection of helicobacter pylori of the present invention comprises box body, is located at the application reagent in the box body, and there is the plastic reaction plate of scrobicula on the surface, Ward door test paper; It is characterized in that: described Ward door test paper is to soak into fully through 1% urea liquid and phenol red indicator, and the test paper that dries again, described application reagent are distilled water and 1% urease solution, and described plastic reaction plate is the plastic reaction plate that there is circular scrobicula on the surface.
Wherein: above-mentioned Ward door test paper is made by following method:
A) urea is added in the deionized water, mixing is made into concentration and is 1% urea liquid, adds phenol red indicator to solution again and is yellow;
B) putting into Ward door test paper makes it to soak fully;
C) treat that test paper soaks into fully after, take out and to dry and be cut into shape and the size that experiment needs.
The application of the reaction box of quick qualitative detecting infection of helicobacter pylori of the present invention, its application process is:
1) use before 1-5 minute, in the reaction plate scrobicula that is placed with the dried test paper of Ward door, add 1-2 and drip distilled water, make urea on the test paper and phenol red soluble in water;
2) get 1% urease solution 1-2 and be added dropwise in the scrobicula of setting, color pulverize redness is as the positive control hole;
The gastric mucosa sample that takes out when 3) gastroscope being detected is put into and is detected scrobicula;
4) solution becomes pink, is shown the positive;
5) if the gastric mucosa sample is shown as the positive, getting final product qualitative is helicobacter pylori infections.
In the application of the reaction box of above-mentioned quick qualitative detecting infection of helicobacter pylori, contain urea in the Ward door test paper of the present invention, run into the helicobacter pylori that can produce urease, promptly generate ammonia and carbon dioxide, thereby change the pH value of reagent, contain in the Ward door test paper simultaneously and can change and the phenol red indicator of variable color with the pH value, therefore, utilize the change color of the reaction box of quick qualitative detecting infection of helicobacter pylori of the present invention, can realize the qualitative detection that HP is infected very easily according to solution.
The reaction box of quick qualitative detecting infection of helicobacter pylori of the present invention compared with prior art has the following advantages and effect:
1, raw material sources are abundant, and are cheap;
2, reaction box is stable, and the used test paper of reaction box is room temperature preservation for a long time, long shelf-life;
3, making and detection step are simple, only need 1 people can finish the whole operation process;
4, qualitative accurate, experiment showed, that accuracy rate is 98.4%;
5, detection speed is fast, and one detection period is 1-10 minute only, has reduced waste of manpower resource.
Embodiment
Below by embodiment, further illustrate outstanding feature of the present invention and marked improvement, but only be the present invention is described and never limit the present invention.
Embodiment 1
Get urea (hydrogen eroxide urea) 50g, adding deionized water 5000ml mixing, to be made into concentration be 1% urea liquid, adds an amount of phenol red indicator to solution and be yellow.Put into soaking into fully that Ward door test paper treats, treat that test paper soaks into fully after, taking-up is dried, it is standby that test paper is cut into disk.
The first using 1-5 minute, in the reaction plate scrobicula that is placed with the dried test paper of Ward door, add 1-2 and drip distilled water, make urea on the test paper and phenol red soluble in water; Get 1% urease solution 1-2 and be added dropwise in the scrobicula of setting, do the positive control hole; Get the gastric mucosa sample that takes out when gastroscope detects and put into the detection scrobicula; Solution becomes pink, is shown the positive; If the gastric mucosa sample is shown as the positive, getting final product qualitative is helicobacter pylori infections.
The positive control hole: visible solution becomes pink, is shown as the positive.
Detect the hole: put into the gastric mucosa sample that certified HP infects positive patients, the pink that also is positive.
Repeated experiments: the result shows that accuracy rate is 98.4%.
In the above-mentioned experiment, Ward door test paper (Whatman paper) and urea (Urea) are provided by Crown Scientific company, and phenol red PH indicator, urease are available from SIGMA company.
Claims (3)
1, a kind of reaction box of quick qualitative detecting infection of helicobacter pylori comprises box body, is located at the application reagent in the box body, and there is the plastic reaction plate of scrobicula on the surface, Ward door test paper; It is characterized in that: described Ward door test paper is to soak into fully through 1% urea liquid and phenol red indicator, and the test paper that dries again, described application reagent are distilled water and 1% urease solution, and described plastic reaction plate is the plastic reaction plate that there is circular scrobicula on the surface.
2, the reaction box of quick qualitative detecting infection of helicobacter pylori according to claim 1 is characterized in that: described Ward door test paper is made by following method:
A) urea is added deionized water, mixing is made into concentration and is 1% urea liquid, adds phenol red indicator to solution again and is yellow;
B) putting into Ward door test paper makes it to soak fully;
C) treat that test paper soaks into fully after, take out and to dry and be cut into shape and the size that experiment needs.
3, the application of the reaction box of claim 1 or 2 described quick qualitative detecting infection of helicobacter pylori, its application process is:
1) use before 1-5 minute, in the reaction plate scrobicula that is placed with the dried test paper of Ward door, add 1-2 and drip distilled water, make urea on the test paper and phenol red soluble in water;
2) get 1% urease solution 1-2 and be added dropwise in the scrobicula of setting, do the positive control hole;
3) get the gastric mucosa sample that takes out when gastroscope detects and put into the detection scrobicula;
4) solution becomes pink, is shown the positive;
5) if the gastric mucosa sample is shown as the positive, getting final product qualitative is helicobacter pylori infections.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610068763 CN1916608A (en) | 2006-09-05 | 2006-09-05 | Reaction box for quick qualitative detecting infection of helicobacter pylori, and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610068763 CN1916608A (en) | 2006-09-05 | 2006-09-05 | Reaction box for quick qualitative detecting infection of helicobacter pylori, and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1916608A true CN1916608A (en) | 2007-02-21 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 200610068763 Pending CN1916608A (en) | 2006-09-05 | 2006-09-05 | Reaction box for quick qualitative detecting infection of helicobacter pylori, and application |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101726581A (en) * | 2008-10-28 | 2010-06-09 | 张丹 | Helicobacter pylori silver nitrate assay kit |
| CN102539424A (en) * | 2011-12-23 | 2012-07-04 | 李雄 | Rapid detection method of urease in tongue coating by using pH value-ethanol method and kit |
| CN103045468A (en) * | 2011-10-14 | 2013-04-17 | 深圳太太基因工程有限公司 | Kit used for detecting helicobacter pylori infection |
| CN103757088A (en) * | 2014-01-20 | 2014-04-30 | 四川万可泰生物技术有限责任公司 | Quantitative determination and drug allergy determination kits for helicobacter pylori viable bacteria and determination method |
| CN104458725A (en) * | 2014-12-08 | 2015-03-25 | 本溪泰斯特捷生物科技有限公司 | Helicobacter pylori urease detection kit and preparation method thereof |
| CN106468663A (en) * | 2016-11-11 | 2017-03-01 | 天益健康科学研究院（镇江）有限公司 | A kind of helicobacter pylori quick detection coupon |
| CN106834412A (en) * | 2015-12-03 | 2017-06-13 | 李和楼 | A kind of method of inspection of helicobacter pylori |
| CN110470663A (en) * | 2019-08-21 | 2019-11-19 | 王冠男 | For quickly detecting the reagent and kit of people's stomach Helicobacter pylori |
| CN112284862A (en) * | 2020-10-23 | 2021-01-29 | 浙江甬誉生物科技有限公司 | Helicobacter pylori staining solution and staining method thereof |
| CN114621995A (en) * | 2022-05-16 | 2022-06-14 | 广州贝思奇诊断试剂有限公司 | Test paper for rapidly detecting gastric helicobacter pylori by using collected tartar as well as preparation method and application of test paper |
-
2006
- 2006-09-05 CN CN 200610068763 patent/CN1916608A/en active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101726581A (en) * | 2008-10-28 | 2010-06-09 | 张丹 | Helicobacter pylori silver nitrate assay kit |
| CN103045468A (en) * | 2011-10-14 | 2013-04-17 | 深圳太太基因工程有限公司 | Kit used for detecting helicobacter pylori infection |
| CN102539424A (en) * | 2011-12-23 | 2012-07-04 | 李雄 | Rapid detection method of urease in tongue coating by using pH value-ethanol method and kit |
| CN103757088A (en) * | 2014-01-20 | 2014-04-30 | 四川万可泰生物技术有限责任公司 | Quantitative determination and drug allergy determination kits for helicobacter pylori viable bacteria and determination method |
| CN103757088B (en) * | 2014-01-20 | 2016-01-20 | 四川万可泰生物技术有限责任公司 | The quantitative assay of helicobacter pylori viable bacteria, drug sensitive detection test kit and measuring method |
| CN104458725A (en) * | 2014-12-08 | 2015-03-25 | 本溪泰斯特捷生物科技有限公司 | Helicobacter pylori urease detection kit and preparation method thereof |
| CN104458725B (en) * | 2014-12-08 | 2017-05-10 | 本溪泰斯特捷生物科技有限公司 | Helicobacter pylori urease detection kit and preparation method thereof |
| CN106834412A (en) * | 2015-12-03 | 2017-06-13 | 李和楼 | A kind of method of inspection of helicobacter pylori |
| CN106468663A (en) * | 2016-11-11 | 2017-03-01 | 天益健康科学研究院（镇江）有限公司 | A kind of helicobacter pylori quick detection coupon |
| CN110470663A (en) * | 2019-08-21 | 2019-11-19 | 王冠男 | For quickly detecting the reagent and kit of people's stomach Helicobacter pylori |
| CN112284862A (en) * | 2020-10-23 | 2021-01-29 | 浙江甬誉生物科技有限公司 | Helicobacter pylori staining solution and staining method thereof |
| CN114621995A (en) * | 2022-05-16 | 2022-06-14 | 广州贝思奇诊断试剂有限公司 | Test paper for rapidly detecting gastric helicobacter pylori by using collected tartar as well as preparation method and application of test paper |
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