CN110468038B - Process for collecting microbial agent by adsorption plate-and-frame filtration method - Google Patents
Process for collecting microbial agent by adsorption plate-and-frame filtration method Download PDFInfo
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- CN110468038B CN110468038B CN201910787622.6A CN201910787622A CN110468038B CN 110468038 B CN110468038 B CN 110468038B CN 201910787622 A CN201910787622 A CN 201910787622A CN 110468038 B CN110468038 B CN 110468038B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/26—Conditioning fluids entering or exiting the reaction vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention discloses a process for collecting microbial agents by an adsorption plate-frame filtration method, wherein a collection part is provided with a rotating piece, the rotating piece is positioned above a plate frame, a motor drives the rotating piece to rotate, and the flow velocity in the collection part is greater than the flow velocity outside the plate frame device to form internal and external pressure difference; a plurality of uniformly arranged plate frames are arranged in the collecting part, and grooves are formed in the first fixing plate and the second fixing plate, so that the collecting plates in the adsorption layer can be conveniently extracted; the adsorption layer is arranged between the first fixing plate and the second fixing plate, strains enter the collection part from the top of the collection part through air flow on the premise of pressure difference, and fall into the collection plate after receiving the strains; according to the bacteria collection process designed by the invention, the collection part is provided with the rotating part and the motor, so that the flow velocity of wind is increased, surrounding microbial strains are driven to enter the collection part, and meanwhile, the two sides of the collection part are provided with the plurality of plate frames for adsorbing and collecting the microbial strains, so that the collection efficiency is increased, and the cost is saved.
Description
Technical Field
The invention relates to the technical field of microbial strain collection, in particular to a process for collecting a microbial strain by an adsorption plate-and-frame filtration method.
Background
The microbial strain is used for distributing feed fermentation strains and biological fertilizer fermentation strains, and the product not only can make up amino acid which is easy to lack in conventional feed, but also can quickly convert the nutrient components of other coarse feed raw materials, thereby achieving the effect of enhancing digestion, absorption and utilization. The research on microbial strains, particularly beneficial bacteria, is in an up-date stage, so that the collection of the microbial strains is also a problem to be solved at present, but the common collection modes of the microbial strains are all natural sedimentation modes, a large number of collection plates are required to be placed in the mode, the number of the collected strains is small, a large amount of time is wasted, the sampling efficiency is low, the labor cost is high, and the method is not suitable for mass collection, so that the production of the process for collecting the microbial strains by using an adsorption plate-and-frame filtration method is an urgent problem to be solved.
Disclosure of Invention
Aiming at the problems in the prior art, the invention aims to provide a process for collecting a microbial agent by an adsorption plate-and-frame filtration method.
The technical scheme adopted by the invention for solving the technical problems is as follows: a process for collecting microbial inoculum by adsorption plate-and-frame filtration comprises
A collection part for collecting strains;
a collecting part for collecting the strains in the collecting part;
an adsorption layer for adsorbing strains entering the collection part;
the collecting part is provided with a rotating piece, the rotating piece is positioned above the plate frame and is suspended at the top of the plate frame device through a connecting piece, the rotating piece is connected with a motor, the motor drives the rotating piece to rotate, the flow velocity in the collecting part is greater than the flow velocity outside the plate frame device, and an internal and external pressure difference is formed;
a plurality of uniformly arranged plate frames are arranged in the collecting part, each plate frame is provided with a first fixing plate and a second fixing plate, and the first fixing plate and the second fixing plate are both provided with grooves, so that the collecting plates in the adsorption layer can be conveniently extracted;
the adsorption layer is arranged between the first fixing plate and the second fixing plate, strains enter the collection part from the top of the collection part through air flow on the premise of pressure difference, and fall into the collection plate after receiving the strains;
the outer wall of collection portion and sheet frame intersection are equipped with the through-hole, and the outer wall through-hole department of collection portion is equipped with the slide rail and follows the gliding baffle of slide rail, and when the thickness of the bacterial in the collecting plate was not higher than first fixed plate, open the baffle that is located the collection portion outer wall along the slide rail this moment, take out the collecting plate to pour the bacterial from the collecting plate.
Specifically, after the strains are extracted from the collecting plates, the collecting plates are disinfected and cleaned and then placed into the adsorption layer, or the collecting plates are replaced with new collecting plates and placed into the adsorption layer, and the strains are collected and collected in a circulating mode.
Specifically, the first fixing plate is of a hollow frame structure, so that the strains fall into the collecting plate.
Specifically, gather portion and collection portion and be and hold the chamber, gather the portion bottom and go deep into collection portion in, and be located the rotating member top.
Specifically, the top of collection portion is open type structure, and is equipped with the mounting to make the bacterial under the pressure difference effect, enter into in the collection portion.
The invention has the following beneficial effects:
according to the bacteria collecting process designed by the invention, the collecting part is provided with the rotating piece and the motor, so that the flow velocity of wind is increased, surrounding microbial strains are driven to enter the collecting part, meanwhile, the two sides of the collecting part are provided with the plurality of plate frames for adsorbing and collecting the microbial strains, meanwhile, the baffle plate is arranged on the outer wall of the plate frame device, so that the collecting plate for collecting the microbial strains can be conveniently extracted, the collecting efficiency is increased, and the cost is saved.
Drawings
FIG. 1 is a flow chart of a process for collecting microbial agents by adsorption plate and frame filtration.
Fig. 2 is a schematic structural diagram of an embodiment of a process for collecting a microbial agent by adsorption plate and frame filtration.
FIG. 3 is a front view of a baffle of a process for collecting a microbial agent by adsorption plate and frame filtration.
FIG. 4 is a top view of a plate and frame of a process for the collection of a microbial agent by adsorption plate and frame filtration.
In the figure: 1-plate frame; 2-a rotating member; 3-a connector; 4-a baffle plate; 5-a fixing piece; 6, a motor; 7-a slide rail; 8-a collecting part; 9-a collecting section; 101-a first fixation plate; 102-a second fixation plate; 103-a collecting plate; 104-grooves.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely further described in detail below with reference to the accompanying drawings in the embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1-4, a process for collecting microbial agents by an adsorption plate-frame filtration method is realized by using a plate-frame device for adsorbing and collecting microbial strains, the plate-frame device is of an upper-lower structure and is composed of a collection part 8 and a collection part 9, the collection part 8 is of a hollow structure, the bottom of the collection part 8 extends into the collection part 9, the collection part 9 is of a sealed structure, the collection part 8 is provided with a rotating part 2, the rotating part 2 is preferably a fan, the rotating part 2 is connected with the top of the collection part 8, particularly is connected with a fixing part 5 at the top of the collection part 8, the fixing part 5 is three rods connected with each other to form a plurality of triangular pie charts, or is two vertical rods, the top of the collection part 8 is divided into 4 parts, or is a rod as the diameter of the collection part 8, and the like, the main purpose of the fixing part 5 is connected with the rotating part 2 through a connecting part 3, and the rotating part 2 is connected with a motor 6, drive swivel member 2 through motor 6 and rotate, form the low pressure air current for air on every side removes to the sheet frame device, and swivel member 2 is located the bottom of collection portion 8, and is equipped with certain distance with the bottom of collection portion 8, the circulation of the air current of being convenient for.
Collecting part 9 is located the lower part of sheet frame device, collecting part 9 is equipped with a plurality of sheet frames 1 of evenly arranging, sheet frame 1 is equipped with first fixed plate 101, second fixed plate 102 and the adsorbed layer that is located between first fixed plate 101 and the second fixed plate 102, be equipped with collecting plate 103 in the adsorbed layer, and the one end of first fixed plate 101 and the one end of second fixed plate 102 all are equipped with recess 104, first fixed plate 101 and second fixed plate 102 are equipped with the recess, be convenient for grasp collecting plate 103, the bottom of first fixed plate 101 and the intersection of second fixed plate 102 are equipped with a plurality of archs, it holds the chamber to form between first fixed plate 101 and second fixed plate 102, first fixed plate 101 is hollow frame structure simultaneously, be convenient for the microbial species to fall on collecting plate 103.
The lengths of the plate frames 1 can be equal, and the lengths of the plate frames 1 from the bottoms of the collection parts 8 to the bottoms of the collection parts 9 are gradually increased, so that the method is more favorable for collecting microbial strains.
The outer wall of collection portion 9 and 1 intersection of sheet frame are equipped with the through-hole, and the outer wall through-hole department of collection portion 9 is equipped with slide rail 7 and follows the gliding baffle 4 of slide rail 7 for sealed sheet frame 1.
A process for collecting microbial agents by an adsorption plate-and-frame filtration method comprises the following steps:
1) placing a plurality of plate frame devices in a region where strains are to be collected, plugging a power supply, and turning on a motor 6 to enable the motor 6 to drive a rotating piece 2 to rotate, wherein the flow velocity in a collecting part 8 is greater than that outside the plate frame devices, so that internal and external pressure difference is formed;
2) under the action of pressure difference, strains enter the collecting part 9 from the top of the collecting part 8 through air flow, and a collecting plate 103 clamped between a first fixing plate 101 and a second fixing plate 102 in the collecting part 9 receives the strains through a frame-type first fixing plate 101;
3) after a period of time, when the thickness of the strains in the collecting plate 103 is observed to be not higher than that of the first fixing plate 101 through the observation port, the baffle plate 4 positioned on the outer wall of the collecting part 9 is opened along the slide rail at the moment, the collecting plate 103 is held at the groove 104 of the first fixing plate 101 and the second fixing plate 102, the collecting plate 103 is taken out, and the strains are poured out of the collecting plate 103;
4) and (4) disinfecting and cleaning the collection plate 103, putting the collection plate into the adsorption layer, or replacing a new collection plate 103, putting the collection plate into the adsorption layer, and circularly collecting and collecting the strains.
During the use, arrange the sheet frame device in the environment that needs gathered the microbial srain, open motor 6, motor 6 drives rotating member 2 and rotates, form directional air current, the microbial srain person in the air current gets into collection portion 9 of sheet frame device, a period of time later, close the motor, open baffle 4 that is located on the outer wall of collection portion 9 through slide rail 7, extract collecting plate 103, place first fixed plate 101 and second fixed plate 102 with new collecting plate 103 again between for continue to collect the microbial srain.
The present invention is not limited to the above embodiments, and any structural changes made under the teaching of the present invention shall fall within the scope of the present invention, which is similar or similar to the technical solutions of the present invention.
The techniques, shapes, and configurations not described in detail in the present invention are all known techniques.
Claims (1)
1. A process for collecting microbial agents by an adsorption plate-and-frame filtration method is characterized by comprising the following steps: comprises that
A collecting part for collecting strains;
a collecting part for collecting the strains in the collecting part;
an adsorption layer for adsorbing the strains entering the collection part;
the collecting part is provided with a rotating part, the rotating part is positioned above the plate frame and is suspended at the top of the plate frame device through a connecting piece, the rotating part is connected with a motor, the motor drives the rotating part to rotate, the flow velocity in the collecting part is greater than that outside the plate frame device, and an internal and external pressure difference is formed;
the collecting part and the collecting part are both accommodating cavities, the bottom of the collecting part extends into the collecting part and is positioned above the rotating part, and the top of the collecting part is of an open structure and is provided with a fixing part so that strains enter the collecting part under the action of pressure difference;
a plurality of uniformly arranged plate frames are arranged in the collecting part, each plate frame is provided with a first fixing plate and a second fixing plate, and the first fixing plate and the second fixing plate are both provided with grooves, so that the collecting plates in the adsorption layer can be conveniently extracted;
the adsorption layer is arranged between the first fixing plate and the second fixing plate, strains enter the collection part from the top of the collection part through air flow on the premise of pressure difference, and after the adsorption layer receives the strains, the first fixing plate is of a hollow frame structure so that the strains fall into the collection plate;
when the thickness of the strains in the collecting plate is not higher than that of the first fixing plate, the baffle plate positioned on the outer wall of the collecting part is opened along the slide rail, the collecting plate is taken out, and the strains are poured out from the collecting plate;
after the strains are extracted from the collecting plates, the collecting plates are disinfected and cleaned and then placed into the adsorption layer, or the collecting plates are replaced by new collecting plates and placed into the adsorption layer, and the strains are collected and collected in a circulating mode.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910787622.6A CN110468038B (en) | 2019-08-26 | 2019-08-26 | Process for collecting microbial agent by adsorption plate-and-frame filtration method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910787622.6A CN110468038B (en) | 2019-08-26 | 2019-08-26 | Process for collecting microbial agent by adsorption plate-and-frame filtration method |
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| Publication Number | Publication Date |
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| CN110468038A CN110468038A (en) | 2019-11-19 |
| CN110468038B true CN110468038B (en) | 2022-09-06 |
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| CN201910787622.6A Active CN110468038B (en) | 2019-08-26 | 2019-08-26 | Process for collecting microbial agent by adsorption plate-and-frame filtration method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CH595445A5 (en) * | 1976-05-20 | 1978-02-15 | Detec Sa | Appts. for collecting microbes suspended in the air |
| CN106836378B (en) * | 2017-03-16 | 2019-03-12 | 中国石油大学(华东) | A kind of vapor liquefaction acquisition device |
| CN208300533U (en) * | 2018-06-19 | 2019-01-01 | 西南林业大学 | A kind of fungi acquisition drying one packet |
| CN210656897U (en) * | 2019-08-26 | 2020-06-02 | 山东锦鸿源生态农业有限责任公司 | Plate frame device for adsorbing and collecting microbial strains |
| CN113462542A (en) * | 2021-08-19 | 2021-10-01 | 辽宁何氏医学院 | Full-automatic air microorganism sampler |
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