CN110467687A - A kind of bletilla striata polyoses glue extraction process - Google Patents
A kind of bletilla striata polyoses glue extraction process Download PDFInfo
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
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Abstract
The invention belongs to Chinese medicine extractive technique fields, and in particular to a kind of bletilla striata polyoses glue extraction process, specifically includes the following steps: (1) adds water that bletilla striata slurries are made after taking the bletilla striata and being crushed, be sieved;(2) bletilla striata slurries carry out first time heating and refluxing extraction after composite bacteria fermentation, obtain extracting solution;(3) extracting solution is filtered, takes filter residue;Then second of heating and refluxing extraction is carried out after the water of 4-5 times of weight is added in filter residue, and is filtered while hot, the first filtrate and the second filter residue are obtained;(4) water of 2.5-3.5 times of weight is added in the second filter residue, and after Ultrasonic Heating refluxing extraction, is filtered while hot, the second filtrate obtained;(5) merge the first filtrate and the second filtrate, and ethyl alcohol is added after being concentrated, stand to carry and collect precipitating;(6) it is drying to obtain after precipitating dehydrated alcohol flushing time.The present invention has the function of improving polysaccharide extract rate relative to traditional bletilla striata extracting method.
Description
Technical field
The invention belongs to Chinese medicine extractive technique field more particularly to a kind of bletilla striata polyoses glue extraction processes.
Background technique
The bletilla striata is orchid family bletilla striata platymiscium, the also known as small bletilla striata, lotus and grass, snow such as end, over the past thousands of years always as tradition
Chinese medicine uses, and major function is sore hemostasis, tonifying lung, detumescence and promoting granulation etc..Contain a large amount of water soluble polysaccharide in bletilla striata bulb,
Its chemical component is mainly glucomannan, and minimum its of molecular weight is the main function ingredient of Bletilla glucomannan.Bletilla striata polyoses glue
A kind of excellent natural thickener, be the higher medical material of safety, performance brilliance pharmaceutic adjuvant and have suitable development
The biomedical material of prospect.In addition, bletilla striata polyoses glue applies also in daily chemical products, chemical thickening agent is substituted, and have
Reduce the functions such as irritation, protection skin, anti-aging.
By promoting the extraction efficiency of the bletilla striata, can make to increase bletilla striata extraction production in the case where not increasing bletilla striata consumption
Object, to meet increasing bletilla striata extract demand.But currently, the public technology extracted about bletilla striata polyoses glue compares
It is few, such as: patent application CN201910217453.2 discloses a kind of bletilla polysaccharide extract and preparation method thereof, disclosed in
The preparation method step of bletilla polysaccharide extract includes: (1) common bletilla processed;(2) bletilla striata Aqueous extracts are made;(3) ethanol precipitation,
It is dry after washing, obtain bletilla striata Thick many candies;(4) bletilla striata Thick many candies are through adding distilled water dissolution, centrifugation, micro-filtration, ultrafiltration, concentration, alcohol precipitation
It dries afterwards to get bletilla polysaccharide extract.Although extract purity that polysaccharide gum through the extraction obtains is high, but recovery rate ratio
Lower, highest recovery rate only has 35.88%;Separately there is patent application CN201710204450.6 to disclose a kind of mentioning for bletilla polysaccharide
Take method, comprising the following steps: (1) prepared by hyacinth bletilla gel;(2) it decolourizes;(3) deproteinized;(4) it purifies, is extracted through this method
Though polysaccharide gum can reduce the destruction caused by polysaccharide, in whole flow process, using decompose cell wall cellulase and
Pectase is respectively the general name of the class of enzymes of decomposition of cellulose and pectin, includes respectively several classes according to function, mechanism of action
Type, and respectively reasonably combined use between different type can produce different effects, the application cellulase and pectin
Although enzyme has the effect of decomposing cell wall, thus for the extracting method for not adding cell wall clastic enzyme, to polysaccharide
The effect of improving of glue recovery rate, but recovery rate is also only 35.12%, illustrates that discomposing effect is still to be improved.
Summary of the invention
It is an object of the invention to: in view of the above problems, provide a kind of bletilla striata for improving bletilla polysaccharide recovery rate
Polysaccharide extracting process.
In order to achieve the above-mentioned object of the invention, The technical solution adopted by the invention is as follows:
A kind of bletilla striata polyoses glue extraction process, comprising the following steps:
(1) take the bletilla striata and crushed, be sieved after obtain common bletilla, 3-5 times of weight is then added into common bletilla
Water stirs to get bletilla striata slurries;
(2) composite bacteria agent of 2-4% is added obtained in step (1) in bletilla striata slurries, and stirs evenly and is placed on 33-
Closed standing for fermentation 16-18h, obtains fermentation liquid under conditions of 37 DEG C, wherein the composite bacteria agent by following parts by weight viable bacteria
Kind composition: 5-9 parts of Neurospora sitophila, 3-6 parts of penicillium variable, 2-5 parts of class alkali fiber sporangium, 3-7 parts of Ralstonia solanacearum and
2-4 parts of bacillus;Then after the protease of 0.7-1.2% volume and the ethyl alcohol of 1-1.5 times of volume are added into fermentation liquid, into
Row first time heating and refluxing extraction, obtains extracting solution;
(3) extracting solution obtained to step (2) is filtered, and is discarded filtrate and is taken its filter residue;Then into obtained filter residue
After the water of 4-5 times of weight is added, second of heating and refluxing extraction is carried out, is filtered while hot, obtains the first filtrate and the second filter
Slag;
(4) water of 2.5-3.5 times of weight is added into the second filter residue that step (3) obtains, is placed in 90-100 DEG C of item
After carrying out Ultrasonic Heating refluxing extraction 1-1.2h under part, it is filtered while hot, obtains the second filter residue and the second filtrate, discard second
Filter residue;
(5) merge the first filtrate that step (3) obtain and the second filtrate that step (4) obtains, obtain third filtrate;Then
Third filtrate is concentrated into the 1/3-1/2 of original volume, obtains concentrate;Then 1-2 times of volume is added into obtained concentrate
90-98% ethanol solution, stir evenly and stand 12-16h under conditions of being placed on 5-10 DEG C, regather precipitating;
(6) it after the precipitating for obtaining step (5) is rinsed 4-6 times with dehydrated alcohol, is dried to water content lower than 10%,
Obtain bletilla polysaccharide.
Further, the mesh number of sieving described in step (1) is 40-100 mesh.
Further, fermentation described in step (2) is carried out under aerobic conditions.
Further, protease described in step (2) is neutral proteinase.
Further, the concentration of ethyl alcohol described in step (2) is 70-95%.
Further, first time heating and refluxing extraction described in step (2) is heated back under conditions of 55-65 DEG C
Stream extracts 1-1.5h.
Further, second of heating and refluxing extraction described in step (3) is heated back under conditions of 60-65 DEG C
Stream extracts 1.5-2h.
Further, Ultrasonic Heating refluxing extraction described in step (4), the ultrasonic power of the ultrasound are 200-
260W。
Further, in step (6), before being deposited in of obtaining is dried, after being first completely dissolved precipitating with deionized water,
The 20% Sevage reagent that mixes with chloroform and n-butanol according to the volume ratio of 5:1 is added, after vibrating centrifugation to it is organic,
Inorganic liquid level intersection is suspended without white, then removes organic layer, collects water layer, the water layer of collection is concentrated into the 1/6- of original volume
It is dried again after 1/5.
Further, the method for the drying is freeze-drying.
In conclusion by adopting the above-described technical solution, the beneficial effects of the present invention are:
The present invention has the function of improving polysaccharide extract rate relative to traditional bletilla striata extracting method, specifically, bletilla striata elder generation powder
It is fermented again with composite bacteria agent after broken, promotes the decomposition of bletilla striata cell wall main component cellulose, pectin and lignin, from
And make bletilla striata dissolution of cellular content, wherein Neurospora sitophila, penicillium variable and class alkali fiber sporangium can divide in fermentation
Cellulase is secreted, plays the role of decomposition to the cellulose components of cell wall, in addition, the Neurospora sitophila is in fermentation process
Lignoenzyme can be secreted, has the function of decomposing to the lignin of one of bletilla striata cell wall main component, and the present invention passes through three
The stringent proportion of class viable bacteria kind amount, so that fermentation process generates three kinds of cellulases and reaches to the decomposition of cellulose and lignin
To preferable synergistic function;And polygalacturonase is secreted during Ralstonia solanacearum, which can pass through effect
In pectin polymer α-Isosorbide-5-Nitrae glycosidic bond and lead to the decomposition of pectin, bacillus can then secrete pectin lyase in fermentation process
Enzyme, the enzyme carry out trans-elimination by the hydrogen on the position C4-C5 of catalysis pectin or the galacturonic acid residues of pectic acid, make
Glycosidic bond is broken and causes the decomposition of pectin, and the Ralstonia solanacearum added in leavening in the present invention and bacillus be not by
Have the function that decompose pectin with approach, and realize have complementary advantages by rational proportion, to improve the decomposition to pectin.
Above it is found that the present invention by it is zymogenic strictly select with proportion to bletilla striata cell wall main component cellulose, lignin with
And pectin plays preferable discomposing effect, to improve the molten rate of intracellular polysaccharide, and cooperates and uses heating and refluxing extraction, ethyl alcohol
Precipitating etc. polysaccharide is damaged it is smaller, therefore have the function that improve bletilla polysaccharide recovery rate.Test result shows through using
Extraction process of the present invention extracts bletilla striata polyoses glue, and the recovery rate of polysaccharide gum is up to 42.88%, and polyoses content is up to 38.61%.
Specific embodiment
Specific embodiments of the present invention will be described in further detail below, but the invention is not limited to these realities
Mode is applied, it is claimed to still fall within the claims in the present invention for any improvement or replacement on the present embodiment essence spirit
Range.
Embodiment 1
A kind of bletilla striata polyoses glue extraction process, comprising the following steps:
(1) it takes the bletilla striata and is crushed, obtains common bletilla after 40 meshes excessively, 3 times of weight are then added into common bletilla
Water, stir to get bletilla striata slurries;
(2) 2% composite bacteria agent is added obtained in step (1) in bletilla striata slurries, and stirs evenly and is placed on 33 DEG C
Aerobic conditions under closed standing for fermentation 16h, obtain fermentation liquid, wherein the composite bacteria agent by following parts by weight viable bacteria kind
Composition: 5 parts of Neurospora sitophila, 3 parts of penicillium variable, 2 parts of class alkali fiber sporangium, 3 parts of Ralstonia solanacearum and bacillus 2
Part;Then after the neutral proteinase of 0.7% volume and 70% ethyl alcohol of 1 times of volume are added into fermentation liquid, it is placed in 55 DEG C of item
First time heating and refluxing extraction is carried out under part takes 1h.
(3) extracting solution obtained to step (2) is filtered, and is discarded filtrate and is taken its filter residue;Then into obtained filter residue
After the water of 4 times of weight is added, second of heating and refluxing extraction 1.5h is carried out under conditions of being placed in 60 DEG C, is filtered, obtains while hot
To the first filtrate and the second filter residue;
(4) water of 2.5 times of weight is added into the second filter residue that step (3) obtains, is carried out under conditions of being placed in 90 DEG C
It after Ultrasonic Heating refluxing extraction 1, is filtered while hot, obtains the second filter residue and the second filtrate, discard the second filter residue, wherein institute
The ultrasonic power for stating ultrasound is 200W;
(5) merge the first filtrate that step (3) obtain and the second filtrate that step (4) obtains, obtain third filtrate;Then
Third filtrate is concentrated into the 1/3 of original volume, obtains concentrate;Then the 90% of 1 times of volume is added into obtained concentrate
Ethanol solution stirs evenly and stands 12h under conditions of being placed on 5 DEG C, regathers precipitating;
(6) after the precipitating for obtaining step (5) is rinsed 4 times with dehydrated alcohol, ethyl alcohol is completely dissolved with deionized water and is rinsed
Precipitating afterwards is subsequently added into the 20% Sevage reagent mixed with chloroform and n-butanol according to the volume ratio of 5:1, through vibrating
After be centrifuged to organic and inorganic liquid level intersection and be suspended without white, then remove organic layer, to slough the residual protein in precipitating,
Water layer is collected, the water layer of collection is concentrated into behind the 1/6 of original volume and is freeze-dried again to water content lower than 10% to get arriving
Bletilla polysaccharide.
Embodiment 2
A kind of bletilla striata polyoses glue extraction process, comprising the following steps:
(1) it takes the bletilla striata and is crushed, obtains common bletilla after 60 meshes excessively, 4 times of weight are then added into common bletilla
Water, stir to get bletilla striata slurries;
(2) 3% composite bacteria agent is added obtained in step (1) in bletilla striata slurries, and stirs evenly and is placed on 35 DEG C
Aerobic conditions under closed standing for fermentation 17h, obtain fermentation liquid, wherein the composite bacteria agent by following parts by weight viable bacteria kind
Composition: 7 parts of Neurospora sitophila, 5 parts of penicillium variable, 4 parts of class alkali fiber sporangium, 5 parts of Ralstonia solanacearum and bacillus 3
Part;Then after the neutral proteinase of 1% volume and 80% ethyl alcohol of 1.2 times of volumes are added into fermentation liquid, it is placed in 60 DEG C of item
First time heating and refluxing extraction is carried out under part takes 1.2h.
(3) extracting solution obtained to step (2) is filtered, and is discarded filtrate and is taken its filter residue;Then into obtained filter residue
After the water of 4.5 times of weight is added, second of heating and refluxing extraction 1.8h is carried out under conditions of being placed in 63 DEG C, is filtered while hot,
Obtain the first filtrate and the second filter residue;
(4) water of 3 times of weight is added into the second filter residue that step (3) obtains, is surpassed under conditions of being placed in 95 DEG C
It after acoustic heating refluxing extraction 1.1h, is filtered while hot, obtains the second filter residue and the second filtrate, discard the second filter residue, wherein institute
The ultrasonic power for stating ultrasound is 240W;
(5) merge the first filtrate that step (3) obtain and the second filtrate that step (4) obtains, obtain third filtrate;Then
Third filtrate is concentrated into the 1/3 of original volume, obtains concentrate;Then 1.5 times of volumes are added into obtained concentrate
94% ethanol solution stirs evenly and stands 14h under conditions of being placed on 8 DEG C, regathers precipitating;
(6) after the precipitating for obtaining step (5) is rinsed 5 times with dehydrated alcohol, ethyl alcohol is completely dissolved with deionized water and is rinsed
Precipitating afterwards is subsequently added into the 20% Sevage reagent mixed with chloroform and n-butanol according to the volume ratio of 5:1, through vibrating
After be centrifuged to organic and inorganic liquid level intersection and be suspended without white, then remove organic layer, to slough the residual protein in precipitating,
Water layer is collected, the water layer of collection is concentrated into behind the 1/6 of original volume and is freeze-dried again to water content lower than 10% to get arriving
Bletilla polysaccharide.
Embodiment 3
A kind of bletilla striata polyoses glue extraction process, comprising the following steps:
(1) take the bletilla striata and crushed, sieve with 100 mesh sieve after obtain common bletilla, then into common bletilla be added 5 times of weight
Water, stir to get bletilla striata slurries;
(2) 4% composite bacteria agent is added obtained in step (1) in bletilla striata slurries, and stirs evenly and is placed on 37 DEG C
Aerobic conditions under closed standing for fermentation 18h, obtain fermentation liquid, wherein the composite bacteria agent by following parts by weight viable bacteria kind
Composition: 9 parts of Neurospora sitophila, 6 parts of penicillium variable, 5 parts of class alkali fiber sporangium, 7 parts of Ralstonia solanacearum and bacillus 4
Part;Then after the neutral proteinase of 1.2% volume and 95% ethyl alcohol of 1.5 times of volumes are added into fermentation liquid, 65 DEG C are placed in
Under the conditions of carry out first time heating and refluxing extraction take 1.5h.
(3) extracting solution obtained to step (2) is filtered, and is discarded filtrate and is taken its filter residue;Then into obtained filter residue
After the water of 5 times of weight is added, second of heating and refluxing extraction 2h is carried out under conditions of being placed in 65 DEG C, is filtered, obtains while hot
First filtrate and the second filter residue;
(4) water of 3.5 times of weight is added into the second filter residue that step (3) obtains, is carried out under conditions of being placed in 100 DEG C
It after Ultrasonic Heating refluxing extraction 1.2h, is filtered while hot, obtains the second filter residue and the second filtrate, discard the second filter residue, wherein
The ultrasonic power of the ultrasound is 260W;
(5) merge the first filtrate that step (3) obtain and the second filtrate that step (4) obtains, obtain third filtrate;Then
Third filtrate is concentrated into the 1/2 of original volume, obtains concentrate;Then the 98% of 2 times of volumes is added into obtained concentrate
Ethanol solution stirs evenly and stands 16h under conditions of being placed on 10 DEG C, regathers precipitating;
(6) after the precipitating for obtaining step (5) is rinsed 6 times with dehydrated alcohol, ethyl alcohol is completely dissolved with deionized water and is rinsed
Precipitating afterwards is subsequently added into the 20% Sevage reagent mixed with chloroform and n-butanol according to the volume ratio of 5:1, through vibrating
After be centrifuged to organic and inorganic liquid level intersection and be suspended without white, then remove organic layer, to slough the residual protein in precipitating,
Water layer is collected, the water layer of collection is concentrated into behind the 1/5 of original volume and is freeze-dried again to water content lower than 10% to get arriving
Bletilla polysaccharide.
Comparative example 1
A kind of bletilla striata polyoses glue extraction process, without containing Neurospora sitophila, change in composite bacteria agent described in step (2)
Mould and class alkali fiber sporangium, remaining is same as Example 2.
Comparative example 2
A kind of bletilla striata polyoses glue extraction process, without containing green withered thunder Er Shi and bud in composite bacteria agent described in step (2)
Spore bacillus, remaining is same as Example 2.
Comparative example 3
A kind of bletilla striata polyoses glue extraction process, composite bacteria agent described in step (2) by following parts by weight viable bacteria kind group
At: 3 parts of Neurospora sitophila, 2 parts of penicillium variable, 1 part of class alkali fiber sporangium, 2 parts of Ralstonia solanacearum and bacillus 1.5
Part, remaining is same as Example 2.
Comparative example 4
A kind of bletilla striata polyoses glue extraction process, composite bacteria agent described in step (2) by following parts by weight viable bacteria kind group
At: 10 parts of Neurospora sitophila, 8 parts of penicillium variable, 6 parts of class alkali fiber sporangium, 9 parts of Ralstonia solanacearum and 5 parts of bacillus,
Remaining is same as Example 2.
Compliance test result
Application value in order to further illustrate the present invention, to what is extracted using the above various embodiments and comparative example
Bletilla striata polyoses glue is tested, wherein embodiment 1-3 is extraction process of the present invention, 1-4 comparative examples of comparative example.
It is extracted respectively by the extraction process of the above various embodiments and comparative example after taking 7 parts of equivalent bletilla striatas, it is respective
It extracts product to mark, and detects the polysaccharide gum recovery rate of each group respectively and polyoses content and be recorded in following table, as a result
It is as follows:
Extraction process | Recovery rate |
Embodiment 1 | 42.23% |
Embodiment 2 | 42.88% |
Embodiment 3 | 41.81% |
Comparative example 1 | 30.53% |
Comparative example 2 | 29.21% |
Comparative example 3 | 32.46% |
Comparative example 4 | 32.13% |
Above data is it is found that the polysaccharide of the bletilla striata polyoses glue recovery rate and gained bletilla striata polyoses glue extracted using the present invention is contained
Amount is superior to other several groups of contrast groups, respectively compares the data of embodiment 2 and comparative example 1 and comparative example 2, can see compound
Without Neurospora sitophila, penicillium variable and class alkali fiber sporangium or without green withered thunder Er Shi and bacillus in microbial inoculum, all can
The recovery rate and polyoses content for reducing bletilla striata polyoses glue illustrate that being added with for composite bacteria agent of the present invention promotes bletilla striata cell disintegration,
To have the function that improve polysaccharide gum recovery rate;And the parts by weight relationship of each viable bacteria inter-species of composite bacteria agent is equal in comparative example 3-4
Not in the parts by weight relation extents of each viable bacteria kind part of the present invention, although still can be by promoting the decomposition of cell wall to improve polysaccharide
Still effect is obviously not so good as the present invention to glue recovery rate, illustrates that the proportion in the present invention in composite bacteria agent between viable bacteria kind has reinforcement
Cell wall breakdown effect, to have the function that the recovery rate for improving bletilla striata polyoses glue;In addition, data also illustrate in table, this hair
The method of bright extraction destroys polysaccharide smaller.
Application value of the invention is proved above.
It is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, Er Qie
In the case where without departing from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, nothing
By from the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended
Claim rather than above description limit, it is intended that by the institute in the meaning and scope for the equivalent loins for falling in claim
It changes and includes within protection scope of the present invention.
Claims (10)
1. a kind of bletilla striata polyoses glue extraction process, which comprises the following steps:
(1) take the bletilla striata and crushed, be sieved after obtain common bletilla, then into common bletilla be added 3-5 times of weight water, stir
It mixes to obtain bletilla striata slurries;
(2) composite bacteria agent of 2-4% is added obtained in step (1) in bletilla striata slurries, and stirs evenly and is placed on 33-37 DEG C
Under conditions of closed standing for fermentation 16-18h, obtain fermentation liquid, wherein the composite bacteria agent by following parts by weight viable bacteria kind group
At: 5-9 parts of Neurospora sitophila, 3-6 parts of penicillium variable, 2-5 parts of class alkali fiber sporangium, 3-7 parts of Ralstonia solanacearum and gemma
2-4 parts of bacillus;Then after the protease of 0.7-1.2% volume and the ethyl alcohol of 1-1.5 times of volume are added into fermentation liquid, the is carried out
Primary heating refluxing extraction, obtains extracting solution;
(3) extracting solution obtained to step (2) is filtered, and is discarded filtrate and is taken its filter residue;Then it is added into obtained filter residue
After the water of 4-5 times of weight, second of heating and refluxing extraction is carried out, is filtered while hot, obtains the first filtrate and the second filter residue;
(4) water of 2.5-3.5 times of weight is added into the second filter residue that step (3) obtains, is placed under conditions of 90-100 DEG C
After carrying out Ultrasonic Heating refluxing extraction 1-1.2h, it is filtered while hot, obtains the second filter residue and the second filtrate, discard the second filter
Slag;
(5) merge the first filtrate that step (3) obtain and the second filtrate that step (4) obtains, obtain third filtrate;Then by
Three filtrates are concentrated into the 1/3-1/2 of original volume, obtain concentrate;Then the 90- of 1-2 times of volume is added into obtained concentrate
98% ethanol solution, stirs evenly and stands 12-16h under conditions of being placed on 5-10 DEG C, regathers precipitating;
(6) precipitating for obtaining step (5) with dehydrated alcohol rinse 4-6 time after, be dried to water content be lower than 10% to get
To bletilla polysaccharide.
2. bletilla polysaccharide extraction process according to claim 1, it is characterised in that: the mesh number of sieving described in step (1)
For 40-100 mesh.
3. bletilla polysaccharide extraction process according to claim 1, it is characterised in that: fermentation is that having described in step (2)
It is carried out under the conditions of oxygen.
4. bletilla polysaccharide extraction process according to claim 1, it is characterised in that: during protease described in step (2) is
Property protease.
5. bletilla polysaccharide extraction process according to claim 1, it is characterised in that: the concentration of ethyl alcohol described in step (2)
For 70-95%.
6. bletilla polysaccharide extraction process according to claim 1, it is characterised in that: step heats for the first time described in (2)
Refluxing extraction is progress heating and refluxing extraction 1-1.5h under conditions of 55-65 DEG C.
7. bletilla polysaccharide extraction process according to claim 1, it is characterised in that: heated for second described in step (3)
Refluxing extraction is progress heating and refluxing extraction 1.5-2h under conditions of 60-65 DEG C.
8. bletilla polysaccharide extraction process according to claim 1, it is characterised in that: Ultrasonic Heating described in step (4) returns
Stream extracts, and the ultrasonic power of the ultrasound is 200-260W.
9. bletilla polysaccharide extraction process according to claim 1, it is characterised in that: in step (6), obtain be deposited in into
Before row is dry, after being first completely dissolved precipitating with deionized water, it is added 20% and is mixed with chloroform and n-butanol according to the volume ratio of 5:1
Made of Sevage reagent, centrifugation is suspended to organic and inorganic liquid level intersection without white after vibrating, then removes organic layer, receives
Collect water layer, the water layer of collection is concentrated into after the 1/6-1/5 of original volume and is dried again.
10. bletilla polysaccharide extraction process according to claim 9, it is characterised in that: the method for the drying is that freezing is dry
It is dry.
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