CN110464872A - A kind of medical aquogel and its preparation method and application - Google Patents
A kind of medical aquogel and its preparation method and application Download PDFInfo
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- CN110464872A CN110464872A CN201910916479.6A CN201910916479A CN110464872A CN 110464872 A CN110464872 A CN 110464872A CN 201910916479 A CN201910916479 A CN 201910916479A CN 110464872 A CN110464872 A CN 110464872A
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- medical aquogel
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- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 150000004676 glycans Chemical class 0.000 claims abstract description 71
- 239000005017 polysaccharide Substances 0.000 claims abstract description 71
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 71
- 235000007119 Ananas comosus Nutrition 0.000 claims abstract description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 20
- 235000018290 Musa x paradisiaca Nutrition 0.000 claims abstract description 19
- 235000006226 Areca catechu Nutrition 0.000 claims abstract description 18
- 244000080767 Areca catechu Species 0.000 claims abstract description 18
- 108010039918 Polylysine Proteins 0.000 claims abstract description 17
- 229920002873 Polyethylenimine Polymers 0.000 claims abstract description 16
- 229920002818 (Hydroxyethyl)methacrylate Polymers 0.000 claims abstract description 15
- WOBHKFSMXKNTIM-UHFFFAOYSA-N Hydroxyethyl methacrylate Chemical compound CC(=C)C(=O)OCCO WOBHKFSMXKNTIM-UHFFFAOYSA-N 0.000 claims abstract description 10
- 230000037314 wound repair Effects 0.000 claims abstract description 10
- 239000002994 raw material Substances 0.000 claims abstract description 9
- 239000000463 material Substances 0.000 claims abstract description 7
- 244000099147 Ananas comosus Species 0.000 claims abstract 6
- 240000008790 Musa x paradisiaca Species 0.000 claims abstract 6
- 239000000017 hydrogel Substances 0.000 claims description 13
- 239000008213 purified water Substances 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 11
- 244000025352 Artocarpus heterophyllus Species 0.000 claims description 7
- 235000008725 Artocarpus heterophyllus Nutrition 0.000 claims description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 239000002002 slurry Substances 0.000 claims description 4
- 108010059892 Cellulase Proteins 0.000 claims description 3
- 229940106157 cellulase Drugs 0.000 claims description 3
- 230000009514 concussion Effects 0.000 claims description 3
- 230000006837 decompression Effects 0.000 claims description 3
- 230000007071 enzymatic hydrolysis Effects 0.000 claims description 3
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims description 3
- 239000000284 extract Substances 0.000 claims description 3
- 239000000706 filtrate Substances 0.000 claims description 3
- 230000001376 precipitating effect Effects 0.000 claims description 3
- OMIGHNLMNHATMP-UHFFFAOYSA-N 2-hydroxyethyl prop-2-enoate Chemical compound OCCOC(=O)C=C OMIGHNLMNHATMP-UHFFFAOYSA-N 0.000 claims description 2
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims description 2
- 241000196324 Embryophyta Species 0.000 claims 1
- 244000300264 Spinacia oleracea Species 0.000 claims 1
- 235000009337 Spinacia oleracea Nutrition 0.000 claims 1
- 235000012907 honey Nutrition 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- 238000005360 mashing Methods 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
- 206010052428 Wound Diseases 0.000 abstract description 17
- 208000027418 Wounds and injury Diseases 0.000 abstract description 17
- 230000008961 swelling Effects 0.000 abstract description 10
- 230000029663 wound healing Effects 0.000 abstract description 8
- 241000588724 Escherichia coli Species 0.000 abstract description 5
- 241000589517 Pseudomonas aeruginosa Species 0.000 abstract description 5
- 241000191967 Staphylococcus aureus Species 0.000 abstract description 4
- 238000010521 absorption reaction Methods 0.000 abstract description 3
- 239000012530 fluid Substances 0.000 abstract description 3
- 230000001408 fungistatic effect Effects 0.000 abstract description 3
- 241000234671 Ananas Species 0.000 description 17
- 241000234295 Musa Species 0.000 description 13
- 230000000052 comparative effect Effects 0.000 description 11
- 206010042674 Swelling Diseases 0.000 description 9
- 239000000243 solution Substances 0.000 description 9
- 230000001954 sterilising effect Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 150000001412 amines Chemical class 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 230000035876 healing Effects 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 206010011985 Decubitus ulcer Diseases 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 241000191940 Staphylococcus Species 0.000 description 1
- 241000256856 Vespidae Species 0.000 description 1
- 239000004964 aerogel Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 230000002951 depilatory effect Effects 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 210000003195 fascia Anatomy 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 150000002466 imines Chemical class 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 239000007928 intraperitoneal injection Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- -1 polyethylene Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 230000008733 trauma Effects 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0014—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials obtained by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0019—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0023—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/0066—Medicaments; Biocides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/008—Hydrogels or hydrocolloids
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J3/00—Processes of treating or compounding macromolecular substances
- C08J3/02—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques
- C08J3/03—Making solutions, dispersions, lattices or gels by other methods than by solution, emulsion or suspension polymerisation techniques in aqueous media
- C08J3/075—Macromolecular gels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/23—Carbohydrates
- A61L2300/232—Monosaccharides, disaccharides, polysaccharides, lipopolysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2379/00—Characterised by the use of macromolecular compounds obtained by reactions forming in the main chain of the macromolecule a linkage containing nitrogen with or without oxygen, or carbon only, not provided for in groups C08J2361/00 - C08J2377/00
- C08J2379/02—Polyamines
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2405/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2401/00 or C08J2403/00
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2477/00—Characterised by the use of polyamides obtained by reactions forming a carboxylic amide link in the main chain; Derivatives of such polymers
- C08J2477/04—Polyamides derived from alpha-amino carboxylic acids
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- Chemical & Material Sciences (AREA)
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Materials Engineering (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Public Health (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Dispersion Chemistry (AREA)
- Polymers & Plastics (AREA)
- Medicinal Chemistry (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Sustainable Development (AREA)
- Materials For Medical Uses (AREA)
- Medicinal Preparation (AREA)
- Compositions Of Macromolecular Compounds (AREA)
Abstract
The present invention provides a kind of medical aquogel and its preparation method and application, and the medical aquogel includes the raw material of following parts by weight: 7~10 parts of hydroxyethyl methacrylate, 6~9 parts of polyethyleneimine, 5~8 parts of epsilon-polylysine, 6~8 parts of banana polysaccharide, 2~4 parts of betel nut polysaccharide, 1~3 part of pineapple polysaccharide and 20~30 parts of water.The present invention uses hydroxyethyl methacrylate, polyethyleneimine, epsilon-polylysine combination banana polysaccharide, betel nut polysaccharide, pineapple polysaccharide, scientific matching, swelling behavior obtained is functional, conducive to the absorption of wound fluid, and there is stronger fungistatic effect to Escherichia coli, staphylococcus aureus and pseudomonas aeruginosa, it is fast to wound healing time, it is strong to wound repair ability, it is more preferable to be applied to prepare wound repair function material.
Description
Technical field
The present invention relates to technical field of hydrogel, in particular to a kind of medical aquogel and its preparation method and application.
Background technique
Wound healing vulnerable to the various factors such as drying, infection, dipping, necrosis, pressure, wound and oedema influence and make
Wound healing becomes difficult.Plain weave yarn is one of most common product of hospital, have the characteristics that it is cheap, ready-to-use, so
And the epidermis newly formed may be torn and cause wound when removing gauze by removing.Ideal wound dressing should absorb extra infiltration
Object and toxin out, keep good moisture between wound and dressing, and protection wound prevents external infection source, prevents wound from sending out
Heat, and there is good permeability to gas, and be easy to remove, wound cannot be damaged.Aerogel dressing application at present
In wound reparation, it is able to solve the above problem.But summer is hot, patient has higher requirements especially to wound repair time, and
Many patients have higher requirements to aesthetic height, most of patient to repairing effect and healing time, existing medical water
Gel is difficult to meet patient demand.
Summary of the invention
Mirror is with this, and the present invention proposes a kind of medical aquogel and its preparation method and application, and the hydrogel is cured wound
The conjunction time is fast, strong to wound repair ability, more preferable to be applied to prepare wound repair function material.
The technical scheme of the present invention is realized as follows:
A kind of medical aquogel, the raw material including following parts by weight: 7~10 parts of hydroxyethyl methacrylate, polyethyleneimine
6~9 parts of amine, 5~8 parts of epsilon-polylysine, 6~8 parts of banana polysaccharide, 2~4 parts of betel nut polysaccharide, 1~3 part of pineapple polysaccharide and water
20~30 parts.
Preferably, the mass ratio of the banana polysaccharide, betel nut polysaccharide and pineapple polysaccharide is 4:2:1.
Preferably, the medical aquogel includes the raw material of following parts by weight: 10 parts of hydroxyethyl methacrylate, polyethylene
8 parts of imines, 7 parts of epsilon-polylysine, 8 parts of banana polysaccharide, 4 parts of betel nut polysaccharide, 2 parts of pineapple polysaccharide and 25 parts of water.
Preferably, the hydrogel preparation method the following steps are included:
(1) banana polysaccharide of above-mentioned parts by weight, betel nut polysaccharide, pineapple polysaccharide are added to water, stir, is made compound more
Sugar juice；
(2) hydroxyethyl methacrylate of above-mentioned parts by weight is added in complex polysaccharide solution, is stirred in 20~25 DEG C
30~40min adds the polyethyleneimine and epsilon-polylysine of above-mentioned parts by weight, in 50~60 DEG C of 2~4h of stirring, wash,
Medical aquogel is made in sterilizing.
Preferably, in step (1), 30~45min is stirred in 2000~3000rpm.
Preferably, in step (2), first time speed of agitator is 3500~4000rpm；Second speed of agitator be 800~
1200rpm。
Preferably, the preparation method of the pineapple polysaccharide the following steps are included: taking jackfruit pulp, beat by addition purified water
Slurry is carried out concussion and extracts 6~8h by slurry, is added 0.3~0.5wt% pectase and 0.5~0.8wt% cellulase, 48~
Filtrate decompression is concentrated for 52 DEG C of enzymatic hydrolysis, centrifugal filtration, and the ethanol solution that volumetric concentration is 90% or more is added in concentrate, from
Heart filtering, takes precipitating, washs, and pineapple polysaccharide is made.
Preferably, the weight ratio of the jackfruit pulp and purified water is 1:3~5.
Preferably, the enzymolysis time is 50~70min.
The described in any item medical aquogels of the claim present invention are applied to prepare wound repair function material.
Compared with prior art, the beneficial effects of the present invention are: the present invention uses hydroxyethyl methacrylate, polyethyleneimine
Amine, epsilon-polylysine combination banana polysaccharide, betel nut polysaccharide, pineapple polysaccharide, scientific matching, swelling behavior performance obtained are good
It is good, conducive to the absorption of wound fluid, and there is stronger suppression to Escherichia coli, staphylococcus aureus and pseudomonas aeruginosa
Bacterium effect, it is fast to wound healing time, it is strong to wound repair ability, it is more preferable to be applied to prepare wound repair function material.In addition,
Using preparation method of the invention, especially with homemade pineapple polysaccharide, the physical property of hydrogel is further increased, and into
The effect of one step improvement wound repairing.
Specific embodiment
In order to be best understood from the technology of the present invention content, specific embodiment is provided below, the present invention is described further.
Experimental method used in the embodiment of the present invention is conventional method unless otherwise specified.
Material used in the embodiment of the present invention, reagent etc., are commercially available unless otherwise specified.
Embodiment 1
A kind of medical aquogel, the raw material including following parts by weight: 7 parts of hydroxyethyl methacrylate, polyethyleneimine 6.2
Part, 5.1 parts of epsilon-polylysine, 6 parts of banana polysaccharide, 2 parts of betel nut polysaccharide, 1 part of pineapple polysaccharide and 20 parts of purified water；Above-mentioned water-setting
The preparation method of glue the following steps are included:
(1) banana polysaccharide of above-mentioned parts by weight, betel nut polysaccharide, pineapple polysaccharide are added to purified water, 2000rpm stirring
Complex polysaccharide solution is made in 45min；
(2) hydroxyethyl methacrylate of above-mentioned parts by weight is added in complex polysaccharide solution, in 3500rpm, 20 DEG C
40min is stirred, the polyethyleneimine and epsilon-polylysine of above-mentioned parts by weight are added, in 800rpm, 50 DEG C of stirring 4h, wash,
Medical aquogel is made in sterilizing.
Embodiment 2
A kind of medical aquogel, the raw material including following parts by weight: 9 parts of hydroxyethyl methacrylate, polyethyleneimine 8.8
Part, 8 parts of epsilon-polylysine, 8 parts of banana polysaccharide, 4 parts of betel nut polysaccharide, 3 parts of pineapple polysaccharide and 30 parts of purified water；Above-mentioned hydrogel
Preparation method the following steps are included:
(1) banana polysaccharide of above-mentioned parts by weight, betel nut polysaccharide, pineapple polysaccharide are added to purified water, 3000rpm stirring
Complex polysaccharide solution is made in 30min；
(2) hydroxyethyl methacrylate of above-mentioned parts by weight is added in complex polysaccharide solution, in 4000rpm, 25 DEG C
30min is stirred, the polyethyleneimine and epsilon-polylysine of above-mentioned parts by weight are added, in 1200rpm, 60 DEG C of stirring 2h, wash,
Medical aquogel is made in sterilizing.
Embodiment 3
A kind of medical aquogel, the raw material including following parts by weight: 10 parts of hydroxyethyl methacrylate, polyethyleneimine 8
Part, 7 parts of epsilon-polylysine, 8 parts of banana polysaccharide, 4 parts of betel nut polysaccharide, 2 parts of pineapple polysaccharide and 25 parts of purified water；Above-mentioned hydrogel
Preparation method the following steps are included:
(1) banana polysaccharide of above-mentioned parts by weight, betel nut polysaccharide, pineapple polysaccharide are added to purified water, 2800rpm stirring
Complex polysaccharide solution is made in 40min；
(2) hydroxyethyl methacrylate of above-mentioned parts by weight is added in complex polysaccharide solution, in 3800rpm, 25 DEG C
35min is stirred, the polyethyleneimine and epsilon-polylysine of above-mentioned parts by weight are added, in 1100rpm, 55 DEG C of stirring 3h, wash,
Medical aquogel is made in sterilizing.
Embodiment 4
The present embodiment and the difference of embodiment 3 be, the pineapple polysaccharide is self-control, preparation method be include following
Step: taking jackfruit pulp, and the purified water that 4 times of weight are added is beaten, and slurry is carried out concussion and extracts 7h, is added
Filtrate decompression is concentrated, will be concentrated by 0.4wt% pectase and 0.65wt% cellulase, 50 DEG C of enzymatic hydrolysis 60min, centrifugal filtration
The ethanol solution that volumetric concentration is 90% or more is added in object, and centrifugal filtration takes precipitating, washs, and pineapple polysaccharide is made.
Comparative example 1
The difference of this comparative example and embodiment 3 is, the medical aquogel, the raw material including following parts by weight: methyl
10 parts of hydroxy-ethyl acrylate, 5 parts of polyethyleneimine, 5 parts of epsilon-polylysine, 5 parts of banana polysaccharide, 5 parts of betel nut polysaccharide, jackfruit are more
10 parts and 30 parts of purified water of sugar.
One, hydrogel made from above-described embodiment and comparative example is taken respectively, detects its swelling ratio, water retention, as a result such as
Under:
(1) swelling ratio measures: it is dry to perseverance to weigh hydrogel 25mg made from Examples 1 to 4 and comparative example 1 respectively
Weight, obtains sample dry weight Wd, it is immersed in PBS buffer solution (pH7.4), is taken out at the time point of setting, blot table with filter paper
It weighs after the moisture in face, obtains the weight in wet base Wt of sample, the quality after 12 hours complete swellings is Ws, and swelling ratio (SR) is counted as the following formula
It calculates: SR (%)=(Wt-Wd)×100/Wd
(2) water retention measures: the hydrogel sample of 1 complete swelling of Examples 1 to 4 and comparative example being put into filter respectively
In the centrifuge tube of net, 500rpm centrifugation after five minutes take out weighing (Wc), be calculated as follows water retention (WR): WR (%)=
Wc×100/Ws
As a result as follows:
| 3h swelling ratio (%) | Water retention (%) | |
| Embodiment 1 | 3528 | 50.52 |
| Embodiment 2 | 3483 | 54.65 |
| Embodiment 3 | 3674 | 55.31 |
| Embodiment 4 | 3954 | 58.46 |
| Comparative example 1 | 3186 | 50.48 |
The above results show to compare comparative example 1, swelling behavior rate and water retention made from the embodiment of the present invention 1~4
It is higher.
Two, bacteriostatic experiment is tested
Escherichia coli, staphylococcus aureus and pseudomonas aeruginosa are cultivated respectively to logarithmic phase, and each 100 μ L that are inoculated with contain bacterium
Number turbidity is 0.5mcf suspension to corresponding agar plate, and coating uniformly, is then respectively adding embodiment and comparative example is made
Hydrogel, every kind of bacterium repeats 3 pieces of plates, and 37 DEG C of cultures for 24 hours, observe result.The diameter for measuring inhibition zone circle, is repeated 3 times
It is averaged.As a result as follows:
The above results show compared to comparative example 1, and hydrogel made from the embodiment of the present invention 1~4 is to Escherichia coli, golden yellow
Color staphylococcus and pseudomonas aeruginosa have more preferably fungistatic effect.
Three, wound healing assay is tested
Experimental rat 60,220~240g of weight, male is randomly divided into 6 groups, every group 10, rat conventinal breeding 3 days
Afterwards, in 2% yellow Jackets of intraperitoneal injection, injection volume 30mL/kg uses the hair for cutting off dorsal area after anaesthetizing successfully, and
Further hair is removed completely with depilatory cream, the round full thickness skin for then surgically cutting off one piece of diameter 1.5cm is formed
The open surface of a wound, as deep as fascia.After trauma model is established, the 1st~5 group of water-setting for using Examples 1 to 4 and comparative example 1 respectively
Glue, right cloth gauze wrapping are fixed, and the 6th group is blank group (directly wrapping up the surface of a wound with gauze), the raising of rat single cage, every dressing in 2 days
Once, until the surface of a wound heals completely, and number of days is recorded, statistics each group is averaged healing days.As a result as follows:
The above results show compared to comparative example 1, using hydrogel made from the embodiment of the present invention 1~4, when wound healing
Between faster, i.e., more promote wound healing.
To sum up, the present invention uses hydroxyethyl methacrylate, polyethyleneimine, epsilon-polylysine combination banana polysaccharide, Bin
Bulky polysaccharide, pineapple polysaccharide, scientific matching, swelling behavior obtained is functional, conducive to the absorption of wound fluid, and it is right
Escherichia coli, staphylococcus aureus and pseudomonas aeruginosa have stronger fungistatic effect, fast to wound healing time, to wound
Face repair ability is strong, more preferable to be applied to prepare wound repair function material.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Within mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of medical aquogel, which is characterized in that the raw material including following parts by weight: 7~10 parts of hydroxyethyl methacrylate,
6~9 parts of polyethyleneimine, 5~8 parts of epsilon-polylysine, 6~8 parts of banana polysaccharide, 2~4 parts of betel nut polysaccharide, pineapple polysaccharide 1~
3 parts and 20~30 parts of water.
2. a kind of medical aquogel according to claim 1, which is characterized in that the banana polysaccharide, betel nut polysaccharide and spinach
The mass ratio of trailing plants honey polysaccharide is 4:2:1.
3. a kind of medical aquogel according to claim 1, which is characterized in that the raw material including following parts by weight: methyl
10 parts of hydroxy-ethyl acrylate, 8 parts of polyethyleneimine, 7 parts of epsilon-polylysine, 8 parts of banana polysaccharide, 4 parts of betel nut polysaccharide, jackfruit are more
2 parts and 25 parts of water of sugar.
4. a kind of medical aquogel according to claim 1, which is characterized in that the preparation method of the hydrogel include with
Lower step:
(1) banana polysaccharide of above-mentioned parts by weight, betel nut polysaccharide, pineapple polysaccharide are added to water, stirred, it is molten that complex polysaccharide is made
Liquid；
(2) hydroxyethyl methacrylate of above-mentioned parts by weight is added in complex polysaccharide solution, in 20~25 DEG C stir 30~
40min adds the polyethyleneimine and epsilon-polylysine of above-mentioned parts by weight, and in 50~60 DEG C of 2~4h of stirring, washing sterilizes,
Medical aquogel is made.
5. a kind of medical aquogel according to claim 4, which is characterized in that in step (1), in 2000~3000rpm
Stir 30~45min.
6. a kind of medical aquogel according to claim 4, which is characterized in that in step (2), first time speed of agitator is
3500~4000rpm；Second of speed of agitator is 800~1200rpm.
7. a kind of medical aquogel according to claim 4, which is characterized in that the preparation method packet of the pineapple polysaccharide
It includes following steps: taking jackfruit pulp, purified water mashing is added, slurry is subjected to concussion and extracts 6~8h, it is added 0.3~
Filtrate decompression is concentrated for 0.5wt% pectase and 0.5~0.8wt% cellulase, 48~52 DEG C of enzymatic hydrolysis, centrifugal filtration, will be dense
The ethanol solution that volumetric concentration is 90% or more is added in contracting object, and centrifugal filtration takes precipitating, washs, and pineapple polysaccharide is made.
8. a kind of medical aquogel according to claim 4, which is characterized in that the weight of the jackfruit pulp and purified water
Amount is than being 1:3~5.
9. a kind of medical aquogel according to claim 4, which is characterized in that the enzymolysis time is 50~70min.
10. medical aquogel according to any one of claims 1 to 9 is applied to prepare wound repair function material.
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