CN101230106A - Preparation method of banana polysaccharide - Google Patents
Preparation method of banana polysaccharide Download PDFInfo
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- CN101230106A CN101230106A CNA200810009334XA CN200810009334A CN101230106A CN 101230106 A CN101230106 A CN 101230106A CN A200810009334X A CNA200810009334X A CN A200810009334XA CN 200810009334 A CN200810009334 A CN 200810009334A CN 101230106 A CN101230106 A CN 101230106A
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- precipitation
- polysaccharide
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- 235000018290 Musa x paradisiaca Nutrition 0.000 title claims abstract description 57
- 150000004676 glycans Chemical class 0.000 title claims abstract description 48
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 48
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 48
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 240000005561 Musa balbisiana Species 0.000 title 1
- 241000234295 Musa Species 0.000 claims abstract description 57
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 36
- 230000007062 hydrolysis Effects 0.000 claims abstract description 20
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 20
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims abstract description 18
- 238000000034 method Methods 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 12
- 239000012043 crude product Substances 0.000 claims abstract description 8
- 239000012530 fluid Substances 0.000 claims abstract description 8
- 239000002253 acid Substances 0.000 claims abstract description 7
- 238000004587 chromatography analysis Methods 0.000 claims abstract description 7
- 239000000706 filtrate Substances 0.000 claims abstract description 7
- 239000012528 membrane Substances 0.000 claims abstract description 7
- BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 claims abstract description 6
- 238000001556 precipitation Methods 0.000 claims description 26
- 239000000243 solution Substances 0.000 claims description 16
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 12
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 9
- 239000000872 buffer Substances 0.000 claims description 7
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 claims description 7
- 239000002953 phosphate buffered saline Substances 0.000 claims description 7
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 6
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 6
- 239000000284 extract Substances 0.000 claims description 6
- 239000002002 slurry Substances 0.000 claims description 6
- 238000001291 vacuum drying Methods 0.000 claims description 6
- 238000012546 transfer Methods 0.000 claims description 5
- 108010019160 Pancreatin Proteins 0.000 claims description 4
- 108090000526 Papain Proteins 0.000 claims description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 4
- 239000004365 Protease Substances 0.000 claims description 4
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 4
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 4
- 239000003513 alkali Substances 0.000 claims description 4
- 238000013375 chromatographic separation Methods 0.000 claims description 4
- 230000014759 maintenance of location Effects 0.000 claims description 4
- 229940055695 pancreatin Drugs 0.000 claims description 4
- 229940055729 papain Drugs 0.000 claims description 4
- 235000019834 papain Nutrition 0.000 claims description 4
- 235000011121 sodium hydroxide Nutrition 0.000 claims description 4
- 206010033546 Pallor Diseases 0.000 claims description 3
- 238000010009 beating Methods 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- 239000004744 fabric Substances 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 238000007654 immersion Methods 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 102000035101 Aspartic proteases Human genes 0.000 claims description 2
- 108091005502 Aspartic proteases Proteins 0.000 claims description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 2
- 235000011114 ammonium hydroxide Nutrition 0.000 claims description 2
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 claims description 2
- 239000000920 calcium hydroxide Substances 0.000 claims description 2
- 229910001861 calcium hydroxide Inorganic materials 0.000 claims description 2
- 235000011116 calcium hydroxide Nutrition 0.000 claims description 2
- 235000013372 meat Nutrition 0.000 claims description 2
- 235000011118 potassium hydroxide Nutrition 0.000 claims description 2
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 2
- 235000017557 sodium bicarbonate Nutrition 0.000 claims description 2
- 238000000967 suction filtration Methods 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims 3
- 238000007710 freezing Methods 0.000 claims 2
- 230000008014 freezing Effects 0.000 claims 2
- 238000005406 washing Methods 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 abstract description 6
- 102000004190 Enzymes Human genes 0.000 abstract description 6
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 238000000605 extraction Methods 0.000 abstract description 5
- 239000007788 liquid Substances 0.000 abstract description 4
- 235000019441 ethanol Nutrition 0.000 abstract 2
- 230000002779 inactivation Effects 0.000 abstract 1
- 229940088598 enzyme Drugs 0.000 description 5
- 238000000746 purification Methods 0.000 description 3
- 208000030507 AIDS Diseases 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- OQUKIQWCVTZJAF-UHFFFAOYSA-N phenol;sulfuric acid Chemical compound OS(O)(=O)=O.OC1=CC=CC=C1 OQUKIQWCVTZJAF-UHFFFAOYSA-N 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- CMSMOCZEIVJLDB-UHFFFAOYSA-N Cyclophosphamide Chemical compound ClCCN(CCCl)P1(=O)NCCCO1 CMSMOCZEIVJLDB-UHFFFAOYSA-N 0.000 description 1
- 206010061598 Immunodeficiency Diseases 0.000 description 1
- 208000029462 Immunodeficiency disease Diseases 0.000 description 1
- 102000015696 Interleukins Human genes 0.000 description 1
- 108010063738 Interleukins Proteins 0.000 description 1
- 241001529936 Murinae Species 0.000 description 1
- 102000057297 Pepsin A Human genes 0.000 description 1
- 108090000284 Pepsin A Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000012445 acidic reagent Substances 0.000 description 1
- 235000021015 bananas Nutrition 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000009509 drug development Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- AZHSSKPUVBVXLK-UHFFFAOYSA-N ethane-1,1-diol Chemical compound CC(O)O AZHSSKPUVBVXLK-UHFFFAOYSA-N 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000007813 immunodeficiency Effects 0.000 description 1
- 230000007365 immunoregulation Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007721 medicinal effect Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- 235000011837 pasties Nutrition 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- 238000010926 purge Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000000452 restraining effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000002893 slag Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
- 239000008215 water for injection Substances 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a preparation method of banana polysaccharide, and the preparation method comprises the following processes in sequence: the banana is washed to be clean, cut to pieces and soaked and processed with acid solution, and then blanched with hot water for a few minutes, cooled, and beaten for stand-by, and then water is added to be extracted. The hydrolysis is performed with double enzyme, and then the enzyme inactivation is performed; the liquid is filtered, and the clear liquid is alcoholized and settled statically over night. The liquid is centrifuged and filtered, the deposit is taken and then washed for two times respectively with 95 percent of ethyl alcohol and acetone, and then dried with vacuum, and the banana polysaccharide crude product is obtained. The banana polysaccharide crude product is taken to be dissolved, centrifuged and filtered with water, and then adsorbed, separated and purified through a DEAE chromatography column on the filtrate, the object is collected and then ultrafilted with ultra filtration membrane, and trapped fluid is collected, alcoholized and settled statically over night. The fluid is centrifuged and filtered, the deposit is washed for two times respectively with 95 percent of ethyl alcohol and acetone, and then dried with vacuum, and the high-purity banana polysaccharide is obtained. The extraction method is simple and safe, no pollution is produced, the yield is high, the production cost is low, the purity of the obtained banana polysaccharide is high, and the preparation method is suitable for the large-scale industrialization production.
Description
Technical field
The present invention relates to the production technique of food, medication chemistry, specifically the preparation method of banana polysaccharide.
Background technology
In the life science field, glycobiology is the research forward position and the focus in this field always.Polysaccharide has many-sided complex biological activity and function, and the sugar chain of polysaccharide can be controlled the division and the differentiation of cell, and the growth of regulating cell is with old and feeble.
Sugar degree is nearly 20% in the ripe banana, and banana polysaccharide descends to the murine interleukin that is caused by endoxan certain restraining effect, and certain immunocompetence is promptly arranged, and this Application and Development for banana polysaccharide provides scientific basis.
Yet banana great majority now just eats as a kind of fruit, and its pharmaceutical use often is left in the basket.Banana polysaccharide has the immunocompetence effect.The harm of AIDS (AIDS) makes people to the serious consequence of immunodeficiency more deep understanding arranged, and therefore seeking the medicine with good immunoregulation effect has become one of developing direction of contemporary new drug development.Therefore, research and development utilizes banana polysaccharide to have vast market prospect.
Contain a lot of organized enzymes in the banana, brown stain takes place easily, it is very big unfavorable that the extraction of banana polysaccharide is had.Because the banana polysaccharide in the banana is very easily oxidized,, make the banana polysaccharide blackout that obtains at last so in the extraction purge process of banana polysaccharide, banana polysaccharide is easy to blackout.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of banana polysaccharide.
The present invention adopts following technical scheme:
(1) with banana wash clean, segment, be 3.0～4.5 acid solution immersion treatment with pH, use 90 ℃～100 ℃ hot water blanching numbers minute again, cooling, making beating are standby;
(2) get standby slurries, adding water is heated to more than 90 ℃ and extracts twice, each 3～4 times of water that add banana meat weight extract, collect extracting solution, cool to room temperature, with sour adjust pH to 3.0～4.0, add aspartic proteases such as papain or stomach en-, after stirring evenly, keep the pH value, 45 ℃～50 ℃ condition hydrolysis 3.0～4.0;
(3), add that pancreatin or trypsinase etc. are neutral, Sumizyme MP, after stirring evenly with extracting solution adjust pH to 9.0～9.5 of alkali after with hydrolysis, keep the pH value 9.0～9.5,45 ℃～50 ℃ condition hydrolysis, enzymolysis finish the post-heating intensification and carry out enzyme-deactivating, are cooled to room temperature;
(4) enzymolysis solution transfers pH to 6.0～6.5 centrifugal with acid, filters, and collects filtrate; Filtrate is carried out alcohol precipitation, standing over night;
(5) solution behind the alcohol precipitation in the step (4) is filtered, get precipitation, precipitation is respectively washed 2 times with 95% ethanol and acetone, and vacuum-drying promptly gets the banana polysaccharide crude product;
(6) get the banana polysaccharide crude product, add the water stirring and dissolve, centrifugal, filter, get filtered solution;
(7) filtered solution is carried out chromatographic separation with the DEAE chromatography column;
(8) target compound after the collection chromatographic separation carries out ultrafiltration with ultra-filtration membrane, collects trapped fluid;
(9) trapped fluid carries out alcohol precipitation, standing over night once more;
(10) solution behind the alcohol precipitation in the step (9) is carried out centrifugal, filter, get precipitation, precipitation is respectively washed 2 times with 95% ethanol and acetone, carries out vacuum-drying, promptly gets the banana polysaccharide elaboration.
The time of enzyme-deactivating and temperature are determined according to the kind of enzyme, generally are heated to 100 ℃, are incubated 5～15 minutes.
The alkali that described adjust pH is used can be sodium hydroxide, calcium hydroxide, potassium hydroxide, sodium bicarbonate or ammoniacal liquor.
The acid that described adjust pH is used can be hydrochloric acid, phosphoric acid, citric acid or sulfuric acid.
With the process that enzyme is hydrolyzed, can grasp the hydrolysis process by the control hydrolysis time, hydrolysis time is short, and the insufficient yield that causes of hydrolysis is low, can extend manufacture cycle again but the hydrolysis time process is long, increases cost and increases contaminated chance.Hydrolysis time can be 450～500 minutes in the general step (2).Equally, hydrolysis time is 200～250 minutes in the described step (3).
The phosphate buffered saline buffer of using during described chromatography is 10～30mmol/L pH5.8～6.8 phosphate buffered saline buffers.
It is the ultra-filtration membrane of 5～10KD that molecular retention is adopted in described ultrafiltration.
The present invention is by carrying out double-enzyme hydrolysis to banana, method through the ethanol alcohol precipitation, other impurity is removed in the first step separation and purification, use DEAE chromatography column absorption and purification then, with molecular retention is that the ultra-filtration membrane of 5～10KD carries out ultrafiltration, collect trapped fluid, pass through the alcohol alcohol precipitation again, other impurity is removed in the second step separation and purification.The advantage of this method is as follows:
1, banana directly extracts banana polysaccharide through production technique such as two enzyme enzymolysis, has advantages such as simple, safety, environmental protection, low cost.
2, the prepared banana polysaccharide purity height of method for preparing banana polysaccharide of the present invention, the yield height, of good quality, but advantages such as mass production.
Advantages such as 3, the used raw materials cost of the preparation method of banana polysaccharide of the present invention is low, and the source is wide, and pericarp, pulp all can extract polysaccharide, and medicinal effect is obvious.
4, preparation is simple, and raw material is easy to get, the reaction conditions gentleness, and the yield height, good stability, also very low to preparation equipment and place requirement, be suitable for large-scale industrial production.
Embodiment
Below by specific embodiment the present invention is described in further detail.
Embodiment:
Get 200 ripe bananas, with the banana wash clean, weigh 6.0kg, segment, be 3.0～4.5 hydrochloric acid soln immersion treatment with pH, use 100 ℃ of hot water blanching 7min again, cooling, making beating are standby.Add 20.01 water and be heated to 100 ℃ of extractions 1.5 hours, reduce to room temperature, filter with filter cloth.The banana slag adds 20.01 water again and is heated to 100 ℃ of extractions 1.5 hours, reduces to room temperature, filters with filter cloth.Collect extracted twice liquid, centrifugal, filter, treat that filtered solution is cooled to 45 ℃～50 ℃, transfer pH to 3.0～4.0 with 30% (w%) citric acid, add the ratio of 10 million international units (than vigor) papain, add 50 million international units (than vigor) papain in every kg slurries, after stirring evenly, keep the condition hydrolysis 8 hours of pH3.0～4.0,45 ℃～50 ℃.With 18% (w%) NaOH the pepsin hydrolysis slurries are transferred pH to 9.0～9.5, the ratio that adds 4 million international units (than vigor) pancreatin in every kg slurries, add 20 million international units (than vigor) pancreatin, after stirring evenly, maintain pH9.0～9.5,45 ℃～50 ℃ hydrolysis 3.5 hours, enzymolysis finishes post-heating and is warming up to 100 ℃, is incubated 15 minutes and carries out enzyme-deactivating, is cooled to below 30 ℃.Transfer pH6.0～6.5 with 30% (w%) citric acid, centrifugal, suction filtration gets supernatant liquor 36.0kg, and alcohol to the determining alcohol that supernatant liquor adds 95% (v/v) is 70%, and standing over night is carried out alcohol precipitation.Centrifugal, filter, get precipitation, precipitation uses 95% (v/v) ethanol and acetone to wash respectively 2 times, drains, and puts drying in the vacuum drying oven, promptly gets banana polysaccharide crude product 268.34g.The banana polysaccharide crude product adds 6.0L water stirring and dissolving, and is centrifugal, filters, and collects filtrate and gets 5.7kg; Transfer pH to 6.8～7.0 with 18%NaOH, standby.With DEAE furnishing pasty state, adopt slurry type method dress post with water for injection, dress column pressure 0.5Mpa.Clean with 400mmol/L pH6.8 phosphate buffered saline buffer earlier behind the dress post, use 20mmol/L pH6.8 phosphate buffered saline buffer balance again, above-mentioned filtrate is gone up the DEAE chromatography column adsorb, with 20mmol/L pH6.8 phosphate buffered saline buffer wash-out.Collect target compound and get 8.9kg, using molecular retention is that the filter membrane of 100K carries out ultrafiltration, collects trapped fluid and gets 7.0kg, centrifugal, filters, and adding 95% alcohol to determining alcohol is 70%, and standing over night is carried out alcohol precipitation.Centrifugal, filter, get precipitation, precipitation is washed 2 times with 95% ethanol and acetone respectively, drains, and puts drying in the vacuum drying oven, promptly gets banana polysaccharide elaboration 20.21g.Assay is carried out in sampling, and detections such as molecular weight determination are standby.
Assay:
1. polysaccharide is differentiated: phenolsulfuric acid reagent react (positive)
2. polysaccharide content: 99.26% (measuring) with the phenolsulfuric acid method
3. polysaccharide molecule flow measurement: 43109 dalton (with reference to two appendix V of pharmacopeia in 2005 H)
Claims (11)
1. the preparation method of a banana polysaccharide, this method is made up of following process successively:
(1) with banana wash clean, segment, be 3.0～4.5 acid solution immersion treatment with pH, use 90 ℃～100 ℃ hot water blanching numbers minute again, cooling, making beating are standby;
(2) get standby slurries, adding water is heated to more than 90 ℃ and extracts twice, each 3～4 times of water that add banana meat weight extract, collect extracting solution, cool to room temperature, with sour adjust pH to 3.0～4.0, add papain or aspartic protease, after stirring evenly, keep the pH value, 45 ℃～50 ℃ condition hydrolysis 3.0～4.0;
(3) with extracting solution adjust pH to 9.0～9.5 of alkali after, add pancreatin or neutrality or Sumizyme MP, after stirring evenly, keep pH value 9.0～9.5 with hydrolysis, 45 ℃～50 ℃ condition hydrolysis, enzymolysis end post-heating carries out enzyme-deactivating, is cooled to room temperature;
(4) transfer pH to 6.0～6.5 with acid, filter, collect filtrate; Filtrate is carried out alcohol precipitation, standing over night;
(5) solution behind the alcohol precipitation in the step (4) is filtered, get precipitation, precipitation is respectively washed with 95% ethanol and acetone, and vacuum-drying promptly gets the banana polysaccharide crude product;
(6) get the banana polysaccharide crude product, add the water stirring and dissolve, centrifugal, filter, get filtered solution;
(7) filtered solution is carried out chromatographic separation with the DEAE chromatography column;
(8) target compound after the collection chromatographic separation carries out ultrafiltration with ultra-filtration membrane, collects trapped fluid;
(9) trapped fluid carries out alcohol precipitation, standing over night once more;
(10) solution behind the alcohol precipitation in the step (9) is carried out centrifugal, filter, get precipitation, each washs precipitation with 95% ethanol and acetone, carries out vacuum-drying, promptly gets the banana polysaccharide elaboration.
2. the preparation method of banana polysaccharide according to claim 1, it is characterized in that: described banana is whole banana, comprises pulp and pericarp.
3. the preparation method of banana polysaccharide according to claim 1, it is characterized in that: the alkali that described adjust pH is used is sodium hydroxide, calcium hydroxide, potassium hydroxide, sodium bicarbonate or ammoniacal liquor.
4. the preparation method of banana polysaccharide according to claim 1, it is characterized in that: the acid that described adjust pH is used is hydrochloric acid, phosphoric acid, citric acid or sulfuric acid.
5. the preparation method of banana polysaccharide according to claim 1 is characterized in that: hydrolysis time is 450～500 minutes in the described step (2).
6. the preparation method of banana polysaccharide according to claim 1 is characterized in that: hydrolysis time is 200～250 minutes in the described step (3).
7. the preparation method of banana polysaccharide according to claim 1 is characterized in that: filtration procedure comprises in the described step (4): carry out coarse filtration with filter cloth earlier, carry out centrifugally again with high speed freezing centrifuge, carry out suction filtration again.
8. the preparation method of banana polysaccharide according to claim 1, it is characterized in that: the filtration procedure in the described step (5) is: centrifugal with high speed freezing centrifuge, pour out supernatant liquor, and collecting precipitation is to container, use 95% ethanol and washing with acetone respectively, drain with suction filter.
9. the preparation method of banana polysaccharide according to claim 1 is characterized in that: the alcohol concn that alcohol precipitation is used in described step (4), (9) is 40%～95%.
10. the preparation method of banana polysaccharide according to claim 1 is characterized in that: the chromatography phosphate buffered saline buffer is 10～30mmol/L in the described step (7), and pH5.8～6.8 phosphate buffered saline buffers carry out wash-out.
11. the preparation method of banana polysaccharide according to claim 1 is characterized in that: it is the ultra-filtration membrane of 5～100KD that molecular retention is adopted in described ultrafiltration.
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| CN200810009334XA CN101230106B (en) | 2008-02-21 | 2008-02-21 | Preparation method of banana polysaccharide |
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| CN200810009334XA CN101230106B (en) | 2008-02-21 | 2008-02-21 | Preparation method of banana polysaccharide |
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| CN101230106A true CN101230106A (en) | 2008-07-30 |
| CN101230106B CN101230106B (en) | 2010-06-09 |
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| CN200810009334XA Expired - Fee Related CN101230106B (en) | 2008-02-21 | 2008-02-21 | Preparation method of banana polysaccharide |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103360506A (en) * | 2013-07-18 | 2013-10-23 | 华南理工大学 | Extraction method of Musa parasdisiac oligosaccharide |
| CN103947968A (en) * | 2014-04-30 | 2014-07-30 | 桂林军供生化技术开发有限公司 | Banana extract, preparation method and application of banana extract |
| JP2015117230A (en) * | 2013-11-14 | 2015-06-25 | 大谷 勝 | Method for producing banana-derived composition, and biologically active substance containing banana-derived composition |
| CN105924541A (en) * | 2016-05-04 | 2016-09-07 | 中国科学院华南植物园 | Method for preparing alpha-1,6-glucan |
| CN108142825A (en) * | 2017-12-22 | 2018-06-12 | 广西兴业百谷米业有限公司 | A kind of dietary fiber rice of high-content and preparation method thereof |
| CN110464872A (en) * | 2019-09-26 | 2019-11-19 | 海南医学院 | A kind of medical aquogel and its preparation method and application |
| CN114230682A (en) * | 2022-02-08 | 2022-03-25 | 中国热带农业科学院分析测试中心 | Functional banana polysaccharide BPF2 and preparation and application thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1337410A (en) * | 2001-09-03 | 2002-02-27 | 王维新 | Production process of extracting fruit polysaccharide from fruits |
| CN100548150C (en) * | 2007-03-09 | 2009-10-14 | 伍曾利 | The preparation method of coconut extractive |
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2008
- 2008-02-21 CN CN200810009334XA patent/CN101230106B/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103360506A (en) * | 2013-07-18 | 2013-10-23 | 华南理工大学 | Extraction method of Musa parasdisiac oligosaccharide |
| CN103360506B (en) * | 2013-07-18 | 2015-10-28 | 华南理工大学 | A kind of extracting method of banana oligosaccharide |
| JP2015117230A (en) * | 2013-11-14 | 2015-06-25 | 大谷 勝 | Method for producing banana-derived composition, and biologically active substance containing banana-derived composition |
| CN103947968A (en) * | 2014-04-30 | 2014-07-30 | 桂林军供生化技术开发有限公司 | Banana extract, preparation method and application of banana extract |
| CN105924541A (en) * | 2016-05-04 | 2016-09-07 | 中国科学院华南植物园 | Method for preparing alpha-1,6-glucan |
| CN105924541B (en) * | 2016-05-04 | 2018-04-17 | 中国科学院华南植物园 | The method that one kind prepares 1,6 glucans of α |
| CN108142825A (en) * | 2017-12-22 | 2018-06-12 | 广西兴业百谷米业有限公司 | A kind of dietary fiber rice of high-content and preparation method thereof |
| CN110464872A (en) * | 2019-09-26 | 2019-11-19 | 海南医学院 | A kind of medical aquogel and its preparation method and application |
| CN114230682A (en) * | 2022-02-08 | 2022-03-25 | 中国热带农业科学院分析测试中心 | Functional banana polysaccharide BPF2 and preparation and application thereof |
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