CN110463532A - A kind of method for culturing seedlings of cabbage - Google Patents
A kind of method for culturing seedlings of cabbage Download PDFInfo
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- CN110463532A CN110463532A CN201910803072.2A CN201910803072A CN110463532A CN 110463532 A CN110463532 A CN 110463532A CN 201910803072 A CN201910803072 A CN 201910803072A CN 110463532 A CN110463532 A CN 110463532A
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- cabbage
- seed
- culturing seedlings
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- black tea
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/15—Leaf crops, e.g. lettuce or spinach
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/10—Fertilisers containing plant vitamins or hormones
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
- C05F5/006—Waste from chemical processing of material, e.g. diestillation, roasting, cooking
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- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Soil Sciences (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention relates to cabbage stalk seedling culturing technical fields, more particularly to a kind of method for culturing seedlings of cabbage, include the following steps: that S1 sows pre-treatment: seed being placed in the naphthalene acid solution that mass concentration is 20-30% after being ultrasonically treated 10-20s, cabbage seed is placed in the heteroauxin solution that mass concentration is 10-15% after being ultrasonically treated 10-20s, cabbage seed is placed in the spermidine solution that mass concentration is 5-8% and is ultrasonically treated 10-20s；S2 adaptability culture: seed, Jacket thickness 2-4mm are wrapped up with the yellow soil of the fermentation cream of slag containing black tea, then stands 1-2 days after being wrapped up with preservative film；The method of the present invention has germination percentage high, and adaptable, growth potential is strong, and disease resistance is strong and the characteristics such as salt tolerant.
Description
Technical field
The present invention relates to cabbage stalk seedling culturing technical fields, and in particular to a kind of method for culturing seedlings of cabbage.
Background technique
Cabbage is the plant of Cruciferae, Btassica, is the mutation also known as cabbage, cabbage, pimple of wild cabbage
White, cabbage, cabbage, cabbage, lotus, small iron head etc. have cold-resistant, disease-resistant, adaptable, easy storage, resistance to fortune, yield
The features such as height, blade are dark green to green, and blade face is smooth, and mesophyll is plump, in good taste, full of nutrition, by the favor of consumer；Knot
Cabbage spends a kind of sedge vegetal pole horn of plenty, and Vitamin C content is up to 1040.00mg/kg (FW), and various content of mineral substances are higher,
Flower a kind of sedge is tender and crisp palatable, removes the peel when edible, prepared food or can both eat raw, and eat fresh and tender tasty and refreshing, excellent taste raw.
Cabbage property likes mild climate, low-temperature-resistant and compared with high temperature resistant, and heading stage suitable temperature is 18-20 DEG C, but
Adaptive temperature range is 7-25 DEG C, and relative moisture of the soil is 70%~80%, and relative air humidity is 80%~90%.And it is sweet
Indigo plant from nutrient growth be transitioned into reproductive growth must by the period of vernalization could bolting, bloom, set seeds, but cabbage seedling
Trophosome (number of sheets of seedling, diameter) to grow to a certain size, living through 12 DEG C of low temperature below of longer term could pass through
The normal size (seed such as sprouted and lesser seedling) of vernalization is not achieved undergoing the low temperature of enough time not in vernalization
Can be by the period of vernalization, and then influence cabbage and blossom and bear fruit.
Summary of the invention
The present invention is in order to solve the above technical problems, provide a kind of method for culturing seedlings of cabbage.
It is realized particular by following technical scheme:
A kind of method for culturing seedlings of cabbage, includes the following steps:
S1 sows pre-treatment: seed being placed in the naphthalene acid solution that mass concentration is 20-30% and is ultrasonically treated 10-20s
Afterwards, cabbage seed is placed in the heteroauxin solution that mass concentration is 10-15% after being ultrasonically treated 10-20s, by balling
Cabbage seed is placed in the spermidine solution that mass concentration is 5-8% and is ultrasonically treated 10-20s；
S2 adaptability culture: with slag containing black tea fermentation cream yellow soil wrap up seed, Jacket thickness 2-4mm, then with guarantor
1-2 days are stood after fresh film package.
Contain calcium 200-300mg, sodium 105-125mg, manganese 30-45mg, molybdenum 5-9mg in every liter of naphthalene acid solution.
In every liter of heteroauxin solution containing sodium 120-130mg, potassium 75-95mg, zinc 25-45mg, magnesium 15-65mg,
Molybdenum 3-7mg.
Contain potassium 120-150mg, nickel 40-80mg, iron 85-105mg, molybdenum 10-18mg, sodium in every liter of spermidine solution
88-108mg。
The frequency being ultrasonically treated in the naphthalene acid solution is 22-28kHz.
The frequency being ultrasonically treated in the heteroauxin solution is 30-38kHz.
The frequency being ultrasonically treated in the spermidine solution is 40-45kHz.
Black tea slag fermentation cream mass percent is 12-18% in the yellow soil of the fermentation of slag containing the black tea cream.
The fermentation of slag containing the black tea cream is after suspension is made in following raw material high-speed stirred, according to (0.5-
1.1)×107The amount of cfu/mL accesses brewer's yeast, and after closed culture 6-7 days, the stearic acid of suspension quality 1-5% is added
The silicone oil mixing of sodium, the xylonic acid sulfonate of 1-3%, 0.3-0.7%: 10-15 parts of CORTEX ZINGIBERIS RHIZOMAE, green onion must 5-9 parts, persimmon leaves 30-
40 parts, 25-28 parts of black tea slag, 80-100 parts of water.
The black tea slag is the waste material waste residue generated in black tea manufacturing process.
The dwell temperature is 26-32 DEG C.
The utility model has the advantages that
The method of the present invention has germination percentage high, and adaptable, growth potential is strong, and disease resistance is strong and the characteristics such as salt tolerant.
It is stood after the present invention wraps up seed with the yellow soil of the fermentation cream of slag containing black tea, then after being wrapped up with preservative film,
And dwell temperature is controlled, so that yellow soil temperature increases, and it is not easy to distribute moisture, is acted in conjunction with microbial activity, promote to have
Effect ingredient enters in cell, improves cell viability, and has achieved the effect that disinfection, inhibited pathogenic microorganism.
The present invention successively uses methyl α-naphthyl acetate, heteroauxin, spermidine solution to impregnate and be ultrasonically treated, and not only has activated cell
Activity, improves balling rate, also shortens the processing time, convenient for Nutrition and Metabolism operating in plant, while utilizing ultrasonication
The variation of intensity improves resistance so that stem thickness is strong.The migration of bind metal ion improves heteroauxin, naphthalene second
The photosensitive properties such as acid, spermidine shorten the processing time, improve treatment effect, enable the quick vernalization of cabbage.
Specific embodiment
Specific embodiments of the present invention will be described in further detail below, but the invention is not limited to these realities
Mode is applied, it is claimed to still fall within the claims in the present invention for any improvement or replacement on the present embodiment essence spirit
Range.
Embodiment 1
A kind of method for culturing seedlings of cabbage, includes the following steps:
S1 sows pre-treatment: seed is placed in the naphthalene acid solution that mass concentration is 20% under the conditions of 22kHz at ultrasound
After managing 10s, cabbage seed is placed in the heteroauxin solution that mass concentration is 10% under the conditions of 30kHz and is ultrasonically treated
After 10s, cabbage seed is placed in the spermidine solution that mass concentration is 5% under the conditions of 40kHz and is ultrasonically treated 10s；
S2 adaptability culture: wrapping up seed with the yellow soil of the fermentation cream of slag containing black tea, Jacket thickness 2mm, then with fresh-keeping
After film package, 1 day is stood under the conditions of 26 DEG C；
Also contain calcium 200mg, sodium 105mg, manganese 30mg, molybdenum 5mg in every liter of naphthalene acid solution；
Also contain sodium 120mg, potassium 75mg, zinc 25mg, magnesium 15mg, molybdenum 3mg in every liter of heteroauxin solution；
Also contain potassium 120mg, nickel 40mg, iron 85mg, molybdenum 10mg, sodium 88mg in every liter of spermidine solution；
Black tea slag fermentation cream mass percent is 12% in the yellow soil of the fermentation of slag containing the black tea cream；
The slag containing black tea fermentation cream is after suspension is made in following raw material high-speed stirred, according to 0.5 ×
107The amount of cfu/mL accesses brewer's yeast, after closed culture 6 days, be added suspension quality 1% odium stearate, 1% it is wooden
Sour sulfonate, 0.3% silicone oil mixing: 10 parts of CORTEX ZINGIBERIS RHIZOMAE, green onion must 5 parts, 30 parts of persimmon leaves, 25 parts of black tea slag, 80 parts of water；
The black tea slag is the waste material waste residue generated in black tea manufacturing process.
Embodiment 2
A kind of method for culturing seedlings of cabbage, includes the following steps:
S1 sows pre-treatment: seed is placed in the naphthalene acid solution that mass concentration is 30% under the conditions of 28kHz at ultrasound
After managing 20s, cabbage seed is placed in the heteroauxin solution that mass concentration is 15% under the conditions of 38kHz and is ultrasonically treated
After 20s, cabbage seed is placed in the spermidine solution that mass concentration is 8% under the conditions of 45kHz and is ultrasonically treated 20s；
S2 adaptability culture: wrapping up seed with the yellow soil of the fermentation cream of slag containing black tea, Jacket thickness 4mm, then with fresh-keeping
After film package, 2 days are stood under the conditions of 32 DEG C；
Also contain calcium 300mg, sodium 125mg, manganese 30-45mg, molybdenum 9mg in every liter of naphthalene acid solution；
Also contain sodium 130mg, potassium 95mg, zinc 45mg, magnesium 65mg, molybdenum 7mg in every liter of heteroauxin solution；
Also contain potassium 150mg, nickel 80mg, iron 105mg, molybdenum 18mg, sodium 108mg in every liter of spermidine solution；
Black tea slag fermentation cream mass percent is 12-18% in the yellow soil of the fermentation of slag containing the black tea cream；
The slag containing black tea fermentation cream is after suspension is made in following raw material high-speed stirred, according to 1.1 ×
107The amount of cfu/mL accesses brewer's yeast, after closed culture 7 days, be added suspension quality 5% odium stearate, 3% it is wooden
Sour sulfonate, 0.7% silicone oil mixing: 15 parts of CORTEX ZINGIBERIS RHIZOMAE, green onion must 9 parts, 40 parts of persimmon leaves, 28 parts of black tea slag, 100 parts of water；
The black tea slag is the waste material waste residue generated in black tea manufacturing process.
Embodiment 3
A kind of method for culturing seedlings of cabbage, includes the following steps:
S1 sows pre-treatment: seed is placed in the naphthalene acid solution that mass concentration is 25% under the conditions of 25kHz at ultrasound
After managing 15s, cabbage seed is placed in the heteroauxin solution that mass concentration is 12% under the conditions of 35kHz and is ultrasonically treated
After 15s, cabbage seed is placed in the spermidine solution that mass concentration is 6% under the conditions of 42kHz and is ultrasonically treated 15s；
S2 adaptability culture: it is 3mm with the yellow soil package seed Jacket thickness of the fermentation cream of slag containing black tea, then uses preservative film
After package, 1 day is stood under the conditions of 28 DEG C；
Also contain calcium 250mg, sodium 115mg, manganese 38mg, molybdenum 7mg in every liter of naphthalene acid solution；
Also contain sodium 125mg, potassium 85mg, zinc 35mg, magnesium 40mg, molybdenum 5mg in every liter of heteroauxin solution；
Also contain potassium 135mg, nickel 60mg, iron 95mg, molybdenum 15mg, sodium 98mg in every liter of spermidine solution；
Black tea slag fermentation cream mass percent is 12-18% in the yellow soil of the fermentation of slag containing the black tea cream；
The slag containing black tea fermentation cream is after suspension is made in following raw material high-speed stirred, according to 0.8 ×
107The amount of cfu/mL accesses brewer's yeast, after closed culture 7 days, be added suspension quality 3% odium stearate, 2% it is wooden
Sour sulfonate, 0.5% silicone oil mixing: 12 parts of CORTEX ZINGIBERIS RHIZOMAE, green onion must 7 parts, 35 parts of persimmon leaves, 26 parts of black tea slag, 90 parts of water；
The black tea slag is the waste material waste residue generated in black tea manufacturing process.
Embodiment 4
A kind of method for culturing seedlings of cabbage, includes the following steps:
S1 sows pre-treatment: seed is placed in the naphthalene acid solution that mass concentration is 25% under the conditions of 25kHz at ultrasound
After managing 15s, cabbage seed is placed in the heteroauxin solution that mass concentration is 12% under the conditions of 35kHz and is ultrasonically treated
After 15s, cabbage seed is placed in the spermidine solution that mass concentration is 6% under the conditions of 42kHz and is ultrasonically treated 15s；
S2 adaptability culture: wrapping up seed with the yellow soil of the fermentation cream of slag containing black tea, Jacket thickness 3mm, then with fresh-keeping
After film package, 1 day is stood under the conditions of 28 DEG C；
Also contain calcium 300mg, sodium 125mg, manganese 30-45mg, molybdenum 9mg in every liter of naphthalene acid solution；
Also contain sodium 120mg, potassium 75mg, zinc 25mg, magnesium 15mg, molybdenum 3mg in every liter of heteroauxin solution；
In every liter of spermidine solution also containing potassium 120-150mg, nickel 40-80mg, iron 85-105mg, molybdenum 10-18mg,
Sodium 88-108mg；
Black tea slag fermentation cream mass percent is 12-18% in the yellow soil of the fermentation of slag containing the black tea cream；
The fermentation of slag containing the black tea cream is after suspension is made in following raw material high-speed stirred, according to (0.5-
1.1)×107The amount of cfu/mL accesses brewer's yeast, and after closed culture 6-7 days, the stearic acid of suspension quality 1-5% is added
The silicone oil mixing of sodium, the xylonic acid sulfonate of 1-3%, 0.3-0.7%: 10-15 parts of CORTEX ZINGIBERIS RHIZOMAE, green onion must 5-9 parts, persimmon leaves 30-
40 parts, 25-28 parts of black tea slag, 80-100 parts of water；
The black tea slag is the waste material waste residue generated in black tea manufacturing process.
Comparative example 1
Difference with embodiment 3 is: calcium 300mg, sodium 125mg, manganese 30- are not contained in every liter of naphthalene acid solution
45mg, molybdenum 9mg.
Comparative example 2
Difference with embodiment 3 is: sodium 120mg, potassium 75mg, zinc are not contained in every liter of heteroauxin solution
25mg, magnesium 15mg, molybdenum 3mg.
Comparative example 3
Difference with embodiment 3 is: in every liter of spermidine solution without containing potassium 120-150mg, nickel 40-80mg,
Iron 85-105mg, molybdenum 10-18mg, sodium 88-108mg.
Comparative example 4
Difference with embodiment 3 is: not utilizing ultrasonic treatment.
Comparative example 5
Difference with embodiment 3 is: molybdenum is not contained in every liter of naphthalene acid solution.
Comparative example 6
Difference with embodiment 3 is: molybdenum is not contained in every liter of heteroauxin solution.
Comparative example 7
Difference with embodiment 3 is: molybdenum is not contained in every liter of spermidine solution.
Test example 1
It tests and is carried out in June, 2017, handle cabbage seed, every area's plantation according to the method for comparative example and embodiment
Area 3m2；Implantation methods are as follows: every area applies potassium sulfate compound fertilizer 500g, covers mulch after herbicide spraying, then moves
It plants.Top dressing 5 times altogether after field planting, the 1st time every cell applies urea 30g；2nd time every cell applies urea 30g, phosphate fertilizer 30g；3rd time every
Cell applies urea 30g, phosphate fertilizer 30g；The 4th often applies urea 35g, phosphate fertilizer 35g；Apply phosphate fertilizer 45g in the every area of the 5th；As a result such as following table
It is shown；
1 each group cabbage plant forms of table compare
Also, the seed of the present inventor's Statistics Implementation example about 5 months, stem thickness reaches 0.8cm, but the seed of comparative example is about
0.8cm can be reached within 7 months.
Test example 2
Examination handles cabbage seed according to the method for comparative example and embodiment, then carries out salt stress processing, observation kind
The salt resistant character of son, test method are as follows: after taking comparative example and embodiment method to handle seed, being placed on and be covered with 6 layers of filter paper, diameter
In the glass tissue culture bottle of 7cm, to put 40 cabbage seeds in each tissue culture bottle, it is repeated 3 times；3mL is added in each tissue culture bottle
The NaCl solution (concentration is respectively 0%, 0.2%, 0.4%, 0.8%, 1.2%) of various concentration, covers tightly bottle cap, is placed in 28 DEG C of temperature
4d is grown under normal illumination (10000lx) in room；It uncaps daily and records the number of 1 chitting piece, count percentage of seedgermination；Knot
Fruit is as shown in table 2；
2 each group cabbage germination percentage of table compares
Claims (10)
1. a kind of method for culturing seedlings of cabbage, which comprises the steps of:
S1 sows pre-treatment: seed is placed in the naphthalene acid solution that mass concentration is 20-30% after being ultrasonically treated 10-20s, it will
Cabbage seed is placed in the heteroauxin solution that mass concentration is 10-15% after ultrasonic treatment 10-20s, by cabbage
Seed is placed in the spermidine solution that mass concentration is 5-8% and is ultrasonically treated 10-20s；
S2 adaptability culture: seed, Jacket thickness 2-4mm are wrapped up with the yellow soil of the fermentation cream of slag containing black tea, then uses preservative film
1-2 days are stood after package.
2. the method for culturing seedlings of cabbage as described in claim 1, which is characterized in that contain calcium in every liter of naphthalene acid solution
200-300mg, sodium 105-125mg, manganese 30-45mg, molybdenum 5-9mg.
3. the method for culturing seedlings of cabbage as described in claim 1, which is characterized in that contain in every liter of heteroauxin solution
Sodium 120-130mg, potassium 75-95mg, zinc 25-45mg, magnesium 15-65mg, molybdenum 3-7mg.
4. the method for culturing seedlings of cabbage as described in claim 1, which is characterized in that contain potassium in every liter of spermidine solution
120-150mg, nickel 40-80mg, iron 85-105mg, molybdenum 10-18mg, sodium 88-108mg.
5. the method for culturing seedlings of cabbage as described in claim 1, which is characterized in that be ultrasonically treated in the naphthalene acid solution
Frequency is 22-28kHz.
6. the method for culturing seedlings of cabbage as described in claim 1, which is characterized in that be ultrasonically treated in the heteroauxin solution
Frequency be 30-38kHz.
7. the method for culturing seedlings of cabbage as described in claim 1, which is characterized in that be ultrasonically treated in the spermidine solution
Frequency is 40-45kHz.
8. the method for culturing seedlings of cabbage as described in claim 1, which is characterized in that the yellow soil of the fermentation of slag containing the black tea cream
Middle black tea slag fermentation cream mass percent is 12-18%.
9. the method for culturing seedlings of cabbage as described in claim 1, which is characterized in that the fermentation of slag containing the black tea cream is will be as follows
After suspension is made in raw material high-speed stirred, according to (0.5-1.1) × 107The amount of cfu/mL accesses brewer's yeast, closed
Culture 6-7 days after, addition the odium stearate of suspension quality 1-5%, the xylonic acid sulfonate of 1-3%, 0.3-0.7% silicone oil
Mixing: 10-15 parts of CORTEX ZINGIBERIS RHIZOMAE, green onion must 5-9 parts, 30-40 parts of persimmon leaves, 25-28 parts of black tea slag, 80-100 parts of water.
10. the method for culturing seedlings of cabbage as described in claim 1, which is characterized in that the dwell temperature is 26-32 DEG C.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112243632A (en) * | 2020-10-15 | 2021-01-22 | 上海市农业科学院 | Processing method and application of aged cauliflower seeds |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106034721A (en) * | 2016-07-12 | 2016-10-26 | 四川农业大学 | Planting method of brassica oleracea sprouting vegetables |
| CN109479637A (en) * | 2018-12-17 | 2019-03-19 | 彭江龙 | A kind of solanaceous vegetables seed seedling-raising method |
-
2019
- 2019-08-28 CN CN201910803072.2A patent/CN110463532A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106034721A (en) * | 2016-07-12 | 2016-10-26 | 四川农业大学 | Planting method of brassica oleracea sprouting vegetables |
| CN109479637A (en) * | 2018-12-17 | 2019-03-19 | 彭江龙 | A kind of solanaceous vegetables seed seedling-raising method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112243632A (en) * | 2020-10-15 | 2021-01-22 | 上海市农业科学院 | Processing method and application of aged cauliflower seeds |
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