CN110448552B - 二氢青蒿素衍生物在制备抗疟药物中的应用 - Google Patents
二氢青蒿素衍生物在制备抗疟药物中的应用 Download PDFInfo
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- CN110448552B CN110448552B CN201910785609.7A CN201910785609A CN110448552B CN 110448552 B CN110448552 B CN 110448552B CN 201910785609 A CN201910785609 A CN 201910785609A CN 110448552 B CN110448552 B CN 110448552B
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Classifications
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Abstract
本发明公开了式I所示的二氢青蒿素衍生物在制备抗疟药物中的应用,拓宽了二氢青蒿素衍生物的制药用途。
Description
技术领域
本发明属于化合物的医药用途技术领域,涉及一类二氢青蒿素衍生物在制备抗疟药物中的应用。
背景技术
疟疾是经按蚊叮咬或输入带疟原虫者的血液而感染疟原虫所引起的虫媒传染病。青蒿素及其衍生物具有高效、快速和低毒的抗疟活性,在全世界范围内得到广泛应用。近年来研究表明,青蒿素及其衍生物还具有抗炎、抗细菌脓毒症、抗组织纤维化和抗肿瘤等多种生物活性。目前对青蒿素的研究主要集中在提高活性、拓宽药效、增加稳定性和溶解性等方面。
发明人所在课题组在过去的研究中,将一些活性小分子或药物引入到二氢青蒿素结构中,设计了多种结构类型的二氢青蒿素衍生物,通过条件的探索,简易、高收率地实现了这些目标化合物的合成,并发现其中部分化合物虽然本身不具备抗菌作用,但其可以作为β-内酰胺类抗生素的抗菌增效剂。
发明内容
本发明的目的在于考察二氢青蒿素衍生物在抗疟方面的活性,以拓宽二氢青蒿素衍生物的制药用途。
经研究,本发明提供如下技术方案:
式I所示的二氢青蒿素衍生物或其消旋体、立体异构体、互变异构体、氮氧化物、药学上可接受的盐在制备抗疟药物中的应用:
式I中,n为1或2;
R1和R2独立地为H、C1-C3烷基或C1-C3羟烷基;
R3为H、C1-C3烷基、C1-C3羟烷基、取代或未取代苯基、叔丁氧羰基、苄氧羰基或脂肪酰基;所述苯基上的取代基为一个或多个,独立选自卤素、羟基、氨基或C1-C3烷基;
R4和R5独立地为H或C1-C3烷基;
R6和R7独立地为H、氨基、羟基或C1-C3烷基;
R8为H、C1-C3烷基、C1-C3烷硫基、取代或未取代苯基;所述苯基上的取代基为一个或多个,独立选自卤素、羟基、氨基或C1-C3烷基。
进一步,式I中,
R1和R2独立地为H、C1-C2烷基或C1-C2羟烷基;
R3为H、C1-C2烷基、C1-C2羟烷基、取代或未取代苯基、叔丁氧羰基、苄氧羰基或烷酰基;
所述苯基上的取代基为一个或多个,独立选自卤素、羟基、氨基或C1-C2烷基;
R4和R5独立地为H或C1-C2烷基;
R6和R7独立地为H、氨基、羟基或C1-C2烷基;
R8为H、C1-C2烷基、C1-C2烷硫基、取代或未取代苯基;所述苯基上的取代基为一个或多个,独立选自卤素、羟基、氨基或C1-C2烷基。
进一步,式I中,
R1和R2独立地为H、C1-C2烷基或C1-C2羟烷基;
R3为H、C1-C2烷基、C1-C2羟烷基、苯基、叔丁氧羰基、苄氧羰基或乙酰基;
R4和R5独立地为H或C1-C2烷基;
R6和R7独立地为H、氨基、羟基或C1-C2烷基;
R8为H、C1-C2烷基、C1-C2烷硫基或苯基。
进一步,式I中,
R1和R2独立地为H、甲基、乙基或羟乙基;
R3为H、甲基、羟乙基、苯基或叔丁氧羰基;
R4和R5独立地为H或甲基;
R6和R7独立地为H或氨基;
R8为H、甲基、甲硫基或苯基。
进一步,式I所示的二氢青蒿素衍生物为以下化合物中的任一种:
进一步,式I所示的二氢青蒿素衍生物为以下化合物中的任一种:1c,1e,1h,2a-1,2a-2,2a-5,2b-1,2b-2,2b-4,2b-5,3a-1,3a-3,3a-6,3b-1,4a-3,4b-1,5b-1,5b-2,5b-5,5b-6。
进一步,所述抗疟药物为抑制恶性疟原虫和/或抑制伯氏疟原虫红外期的药物。
除另有说明外,本发明中的术语“消旋体”是指由等量对映体构成的光学不活性的有机物。“立体异构体”是指原子组成及键接相同而原子在三维空间排列上不同的分子。“互变异构体”是指因分子中某一原子在两个位置迅速移动而产生的官能团异构体。“氮氧化物”是指三级氮连接氧原子形成+N-O-结构单元的有机物。“药学上可接受的盐”可以是酸性盐,也可以是碱性盐,例如无机酸盐、有机酸盐、无机碱盐或有机碱盐。
本发明的有益效果在于:本发明公开了式I所示的二氢青蒿素衍生物在制备抗疟药物中的应用,拓宽了二氢青蒿素衍生物的制药用途。
具体实施方式
为了使本发明的目的、技术方案和有益效果更加清楚,下面将对本发明的优选实施例进行详细的描述。
优选实施例中使用的主要试剂及规格:二甲胺、二乙胺、乙醇胺、氮甲基乙醇胺、二乙醇胺、吡咯烷、哌啶、吗啉、乙腈、二氯甲烷(重庆化学试剂总厂,AR);二氢青蒿素(重庆华立武陵山制药有限公司,AR);溴乙醇(上海达瑞精细化工有限公司,AR);无水哌嗪、N-甲基哌嗪、N-羟乙基哌嗪、N-Boc-哌嗪、N-苯基哌嗪、3-溴-1-丙醇、咪唑、2-甲基咪唑、4-甲基咪唑、吡唑、3,5-二甲基吡唑、苯并咪唑、1,2,3-三氮唑、1,2,4-三氮唑、苯并三氮唑、3-氨基-1,2,4-三氮唑、3-氨基-5-巯基-1,2,4-三氮唑、1-H-四氮唑、5-甲基四氮唑、5-苯基四氮唑、5-甲巯基四氮唑(上海达瑞精细化工有限公司,>98%);1-羟基-苯并三氮唑(上海共价化学科技公司,工业级);46.5%BF3.Et2O(上海晶纯试剂有限公司,AR);其余试剂均为市售化学纯或分析纯产品,未经纯化直接使用。
优选实施例中使用的主要仪器及型号:精密显微熔点测定仪(X-6,北京福凯仪器有限公司);数字式自动旋光仪(WZZ-2S,上海精密科学仪器有限公司);超导核磁共振波谱仪(AV-300,Bruker,瑞士);高分辨质谱仪(HR ESI MS)(Varian7.0T,Varian,USA)。
实施例1、DHA胺类衍生物的合成
1、DHA脂肪胺类衍生物1的合成
DHA脂肪胺类衍生物1a-1h按照文献(Chong Wu,et al.Design,Synthesis andEvaluation of the Antibacterial Enhancement Activities of AminoDihydroartemisinin Derivatives.Molecules,2013,18,6866-6882)中所述化合物4a-4u的制备方法进行制备。
2、DHA哌嗪类衍生物2的合成
1)中间体M1的合成
中间体M1按照中国专利104418864B(双氢青蒿素与喹诺酮类化合物的偶联物及其制备方法和应用)中所述中间体IM1和IM2的制备方法进行制备。
2)DHA哌嗪类衍生物2a-1~2a-5及2b-1~2b-5的合成
在100mL圆底烧瓶中依次加入M1、CH3CN、K2CO3及哌嗪或取代哌嗪,控温搅拌反应,TLC监测反应进程。反应结束后,加入CH2Cl2 15mL和饱和NaCl水溶液20mL,静置分层,水层用CH2Cl2(10mL×2)萃取,合并有机相,饱和食盐水20mL洗涤,无水Na2SO4干燥,减压旋蒸除去CH2Cl2得粗品或纯品,必要时柱层析,干燥,即得目标化合物2。具体合成条件及结果见表1。
表1目标化合物2的合成条件及结果
目标化合物2表征数据如下:
2a-1:1H NMR(300MHz,CDCl3)δ:0.90(3H,d,J=7.5Hz,H-13),0.95(3H,d,J=6.6Hz,H-14),1.43(3H,s,H-15),1.22-2.06(10H,m,H-2,H-3,H-7~H-10),2.37-2.60(12H,m,H-1,H-11,H-17~H-19),3.52-3.59(1H,m,H-16),3.91-3.98(1H,m,H-16),4.80(1H,s,H-12),5.45(1H,s,H-5).
2a-2:1H NMR(300MHz,CDCl3)δ:0.90(3H,d,J=7.5Hz,H-13),0.96(3H,d,J=6.6Hz,H-14),1.43(3H,s,H-15),1.46(9H,s,H-22),1.22-2.06(10H,m,H-2,H-3,H-7~H-10),2.30(3H,s,H-20),2.37-2.60(12H,m,H-1,H-11,H-17~H-19),3.52-3.59(1H,m,H-16),3.91-3.98(1H,m,H-16),4.80(1H,s,H-12),5.45(1H,s,H-5).
2a-3:黄色油状物;(c 2.5mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.89(3H,d,J=7.3Hz,H-13),0.95(3H,d,J=6.0Hz,H-14),1.42(3H,s,H-15),1.15-2.06(10H,m,H-2,H-3,H-7~H-10),2.28-2.59(14H,m,H-1,H-11,H-17~H-20),3.36-4.43(1H,m,H-16),3.62(2H,t,J=5.3Hz,H-21),3.84-3.91(1H,m,H-16),4.77(1H,d,J=3.3Hz,H-12),5.30(1H,s,H-22),5.38(1H,s,H-5);13C NMR(75MHz,CDCl3)δ:103.98(C-4),101.90(C-12),87.84(C-5),81.07(C-6),68.54(C-16),59.36(C-22),57.72(C-20),55.52(C-17),53.26(C-19),52.45(C-1),46.76(C-18),44.31(C-7),37.39(C-11),36.32(C-10),34.57(C-3),30.76(C-9),26.12(C-8),24.63(C-15),24.30(C-2),20.29(C-14),13.01(C-13).HRMS:C23H40N2O6[M+H]+计算值441.2959,测定值441.2954.
2a-4:m.p.:107.8-109.1℃;(c 1.0mg/mL,CH2Cl2).1H NMR(300MHz,CDCl3)δ:0.90(3H,d,J=7.2Hz,H-13),0.96(3H,d,J=6.0Hz,H-14),1.43(3H,s,H-15),1.46(9H,s,H-22),1.22-2.06(10H,m,H-2,H-3,H-7~H-10),2.32-2.65(8H,m,H-1,H-11,H-17 and H-18),3.40-3.44(4H,m,H-19),3.52-3.58(1H,m,H-16),3.92-4.00(1H,m,H-16),4.80(1H,s,H-12),5.46(1H,s,H-5);13C NMR(75MHz,CDCl3)δ:154.6(C-21),104.0(C-4),101.9(C-12),87.8(C-5),81.0(C-6),79.7(C-22),66.0(C-16),57.9(C-17),53.5(C-19),52.0(C-1),49.2(C-18),44.4(C-7),37.4(C-11),36.4(C-10),34.6(C-3),30.8(C-9),28.4(C-23),26.4(C-17),26.1(C-8),24.6(C-15),24.4(C-2),20.4(C-14),13.0(C-13).HR MS:C27H40N2O5[M+H]+计算值497.3221,测定值497.3222.
2a-5:m.p.:90.0-91.7℃;(c 1.0mg/mL,CH2Cl2).1H NMR(300MHz,CDCl3)δ:0.90(3H,d,J=7.2Hz,H-13),0.95(3H,d,J=6.0Hz,H-14),1.44(3H,s,H-15),1.23-2.06(10H,m,H-2,H-3,H-7~H-10),2.32-2.42(1H,m,H-1),2.59-2.70(7H,m,H-11,H-17 and H-18),3.21(4H,t,J=7.5Hz,H-19),3.58-3.65(1H,m,H-16),3.98-4.05(1H,m,H-16),4.83(1H,d,J=2.7Hz,H-12),5.48(1H,s,H-5),6.89(1H,t,J=7.2Hz,H-23),6.94(2H,d,J=7.8Hz,H-21),7.25-7.30(2H,m,H-22);13C NMR(75MHz,CDCl3)δ:151.3(C-20),129.1(C-22),120.0(C-23),116.0(C-21),104.0(C-4),102.0(C-12),87.9(C-5),81.1(C-6),66.0(C-16),57.9(C-17),53.5(C-19),52.6(C-1),49.2(C-18),44.4(C-7),37.5(C-11),36.4(C-10),34.7(C-3),30.9(C-9),26.2(C-8),24.7(C-15),24.4(C-2),20.3(C-14),13.1(C-13).HR MS:C27H40N2O5(M+H)+计算值473.3010,测定值473.3014.
2b-1:黄色油状物;(c 2.5mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.89(3H,d,J=7.2Hz,H-13),0.96(3H,d,J=6.0Hz,H-14),1.44(3H,s,H-15),1.26-2.06(12H,m,H-2,H-3,H-7~H-10 and H-17),2.37-2.62(12H,m,H-1,H-11,H-18~H-20),3.37-3.44(1H,m,H-16),3.84-3.92(1H,m,H-16),4.77(1H,s,H-12),5.39(1H,s,H-5).
2b-2:黄色油状物;(c 1.2mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.89(3H,d,J=7.2Hz,H-13),0.96(3H,d,J=6.0Hz,H-14),1.44(3H,s,H-15),1.26-2.06(12H,m,H-2,H-3,H-7~H-10 and H-17),2.30(3H,s,H-20),2.37-2.62(12H,m,H-1,H-11,H-18~H-20),3.37-3.44(1H,m,H-16),3.84-3.92(1H,m,H-16),4.77(1H,s,H-12),5.39(1H,s,H-5);13C NMR(75MHz,CDCl3)δ:104.1(C-4),101.9(C-12),87.8(C-5),81.0(C-6),66.5(C-16),55.5(C-18),55.0(C-19),53.1(C-20),52.5(C-21),46.0(C-1),44.4(C-7),37.1(C-11),36.4(C-10),34.6(C-3),30.9(C-9),27.0(C-17),26.2(C-8),24.6(C-15),24.4(C-2),20.3(C-14),13.0(C-13).HR MS:C23H40N2O5[M+H]+计算值425.3010,测定值425.3010.
2b-3:黄色油状物;(c 2.5mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.89(3H,d,J=7.3Hz,H-13),0.95(3H,d,J=6.0Hz,H-14),1.42(3H,s,H-15),1.15-2.06(12H,m,H-2,H-3,H-7~H-10 and H-17),2.28-2.59(14H,m,H-1,H-11,H-17~H-20),3.36-3.43(1H,m,H-16),3.62(2H,t,J=5.3Hz,H-21),3.84-3.91(1H,m,H-16),4.77(1H,d,J=3.3Hz,H-12),5.30(1H,s,H-23),5.38(1H,s,H-5);13C NMR(75MHz,CDCl3)δ:104.00(C-4),101.90(C-12),87.81(C-5),81.04(C-6),66.43(C-16),59.31(C-22),57.68(C-20),55.42(C-17),53.06(C-19),52.76(C-18),52.49(C-1),44.35(C-7),37.42(C-11),36.35(C-10),34.56(C-3),30.84(C-9),26.97(C-17),26.12(C-8),24.61(C-15),24.40(C-2),20.30(C-14),12.97(C-13).HR MS:C24H42N2O6[M+H]+计算值455.3116,测定值455.3115.
2b-4:黄色油状物;(c 1.5mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.90(3H,d,J=7.5Hz,H-13),0.96(3H,d,J=6.0Hz,H-14),1.43(3H,s,H-15),1.47(9H,s,H-23),1.26-2.06(12H,m,H-2,H-3,H-7~H-10 and H-17),2.32-2.43(1H,m,H-1),2.61-2.64(1H,m,H-11),3.40-3.46(11H,m,H-16,H-18~H-20),3.92-4.00(1H,m,H-16),4.19(2H,t,J=6.3Hz,H-14),4.76(1H,s,H-12),5.37(1H,s,H-5);13C NMR(75MHz,CDCl3)δ:154.6(C-21),104.0(C-4),101.9(C-12),87.8(C-5),81.0(C-6),79.7(C-22),66.5(C-16),55.3(C-18),52.6(C-20),52.5(C-19),52.0(C-1),44.4(C-7),37.4(C-11),36.4(C-10),34.6(C-3),30.8(C-9),28.4(C-23),26.4(C-17),26.1(C-8),24.6(C-15),24.4(C-2),20.4(C-14),13.0(C-13).HR MS:C27H46N2O7[M+H]+计算值511.3378,测定值511.3370.
2b-5:1H NMR(300MHz,CDCl3)δ:0.92(3H,d,J=7.5Hz,H-13),0.96(3H,d,J=5.7Hz,H-14),1.44(3H,s,H-15),1.23-2.06(12H,m,H-2,H-3,H-7~H-10 and H-17),2.32-2.42(1H,m,H-1),2.49-2.65(7H,m,H-11,H-18 and H-19),3.22-3.25(4H,m,H-20),3.40-3.48(1H,m,H-16),3.88-3.96(1H,m,H-16),4.80(1H,d,J=2.4Hz,H-12),5.41(1H,s,H-5),6.87(1H,t,J=7.2Hz,H-23),6.94(2H,d,J=7.8Hz,H-21),7.25-7.30(2H,m,H-22);13C NMR(75MHz,CDCl3)δ:151.2(C-21),129.1(C-23),119.7(C-24),116.0(C-22),104.0(C-4),102.0(C-12),87.9(C-5),81.1(C-6),66.5(C-16),55.6(C-18),53.2(C-20),52.6(C-1),49.1(C-19),44.4(C-7),37.5(C-11),36.4(C-10),34.6(C-3),30.9(C-9),28.4(C-17),26.2(C-8),24.7(C-15),24.5(C-2),20.4(C-14),13.0(C-13).
HR MS:C28H42N2O5[M+H]+计算值487.3167,测定值487.3162.
实施例2、DHA唑类衍生物的合成
在100mL圆底烧瓶中依次加入唑类化合物YH、N,N-二甲基甲酰胺(DMF)和碱(NaH或K2CO3),搅拌15min后,加入M1,控温搅拌反应,TLC监测反应进程。反应完成后,加入乙酸乙酯(EtOAc)15mL和饱和NaCl水溶液20mL,静置分层,水层用EtOAc(10mL×2)萃取,合并有机相,饱和食盐水20mL洗涤,无水Na2SO4干燥,减压旋蒸除去EtOAc得粗品或纯品,必要时柱层析,干燥,即得目标化合物3,4,5。具体合成条件及结果见表2,3,4。
表2目标化合物3的合成条件及结果
表3目标化合物4的合成条件及结果
表4目标化合物5的合成条件及结果
目标化合物3-5的表征数据如下:
3a-1:m.p.:93.7-95.1℃;(c 1.0mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.80(3H,d,J=7.4Hz,H-14),0.93(3H,d,J=5.5Hz,H-13),1.42(3H,s,H-15),1.17-2.08(10H,m,H-2,H-3,H-7~H-10),2.34(1H,td,J=14.0,3.9Hz,H-1),2.51-2.65(1H,m,H-11),3.68-3.81(1H,m,H-16),4.19-4.41(3H,m,H-16 and H-17),4.75(1H,d,J=3.4Hz,H-12),5.13(1H,s,H-5),6.23(s,1H,H-19),7.42(1H,s,H-20),7.51(1H,s,H-20);13CNMR(75MHz,CDCl3)δ:139.29(C-20),129.67(C-19),105.16(C-18),103.98(C-4),101.87(C-12),87.70(C-5),80.89(C-6),66.66(C-16),52.37(C-1),51.99(C-17),44.10(C-7),37.11(C-11),36.29(C-10),34.46(C-3),30.62(C-9),26.08(C-8),24.57(C-15),24.14(C-2),20.29(C-14),12.81(C-13).HR MS:C20H30N2O5(M+Na)+计算值401.2047,测定值401.2050.
3a-2:黄色油状物;(c 1.1mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.85(3H,d,J=7.2Hz,H-14),0.94(3H,d,J=6.1Hz,H-13),1.44(3H,s,H-15),1.22-2.07(10H,m,H-2,H-3,H-7~H-10),2.32-2.38(1H,m,H-1),2.61-2.63(1H,m,H-11),3.62-3.68(1H,m,H-16),4.15-4.21(3H,m,H-16 and H-17),4.76(1H,s,H-12),5.11(1H,s,H-5),7.00(1H,s,H-19),7.09(1H,s,H-18),7.62(1H,s,H-20);13C NMR(75MHz,CDCl3)δ:128.7(C-20),123.1(C-19),118.9(C-18),104.1(C-4),102.0(C-12),87.8(C-5),80.8(C-6),67.0(C-16),52.3(C-1),47.2(C-17),44.0(C-7),37.2(C-11),36.3(C-10),34.4(C-3),30.6(C-9),26.1(C-8),24.6(C-15),24.3(C-2),20.3(C-14),13.0(C-13).HR MS:C20H30N2O5(M+H)+计算值379.2228,测定值379.2221.
3a-3:黄色油状物;(c 1.1mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.91(3H,d,J=6.9Hz,H-14),0.95(3H,d,J=6.3Hz,H-13),1.45(3H,s,H-15),1.19-2.06(10H,m,H-2,H-3,H-7~H-10),2.12(3H,s,H-21),2.32-2.43(1H,m,H-1),2.59-2.66(1H,m,H-11),3.40-3.52(4H,m,H-16 and H-17),4.69(1H,d,J=2.7Hz,H-12),5.39(1H,s,H-5);13CNMR(75MHz,CDCl3)δ:127.8(C-18),127.2(C-20),118.8(C-19),104.2(C-4),102.0(C-12),87.8(C-5),80.9(C-6),66.9(C-16),52.4(C-1),47.8(C-17),44.0(C-7),37.2(C-11),36.3(C-10),34.4(C-3),30.6(C-9),26.1(C-8),24.6(C-15),24.2(C-2),20.3(C-14),13.0(C-13),12.7(C-21).HR MS:C21H32N2O5(M+H)+计算值393.2384,测定值393.2382.
3a-4:黄色油状物;(c 1.1mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.85(3H,d,J=7.2Hz,H-14),0.94(3H,d,J=4.8Hz,H-13),1.43(3H,s,H-15),1.22-2.07(10H,m,H-2,H-3,H-7~H-10),2.29-2.34(1H,m,H-1),2.41(3H,s,H-21),2.61-2.63(1H,m,H-11),3.58-3.63(1H,m,H-16),3.99-4.16(3H,m,H-16 and H-17),4.76(1H,d,J=3.3Hz,H-12),5.08(1H,s,H-5).6.88(1H,s,H-19),6.92(1H,s,H-18);13C NMR(75MHz,CDCl3)δ:126.7(C-20 and C-19),118.9(C-18),104.2(C-4),102.0(C-12),87.8(C-5),80.9(C-6),66.8(C-16),52.4(C-1),45.8(C-17),44.0(C-7),37.2(C-11),36.3(C-10),34.4(C-3),30.6(C-9),26.1(C-8),24.6(C-15),24.2(C-2),20.3(C-14),13.0(C-13),12.9(C-21).HR MS:C21H32N2O5(M+H)+计算值393.2384,测定值393.2386.
3a-5:黄色油状物;(c 1.0mg/mL,CH2Cl2).1H NMR(300MHz,CDCl3)δ:0.81(3H,d,J=7.2Hz,H-14),0.87(3H,d,J=6.3Hz,H-13),1.42(3H,s,H-15),1.26-2.08(10H,m,H-2,H-3,H-7~H-10),2.29-2.37(1H,m,H-1),2.21(3H,s,H-21),2.28(3H,s,H-22),2.51-2.59(1H,m,H-11),3.64-3.69(1H,m,H-16),4.12-4.28(3H,m,H-16 and H-17),4.75(1H,d,J=2.7Hz,H-12),5.07(1H,s,H-5).5.86(1H,s,H-19);13C NMR(75MHz,CDCl3)δ:147.4(C-20),139.4(C-19),104.6(C-4),103.9(C-19),101.7(C-12),87.6(C-5),80.9(C-6),66.4(C-16),52.3(C-1),47.9(C-17),44.2(C-7),37.0(C-11),36.3(C-10),34.6(C-3),30.7(C-9),26.1(C-8),24.5(C-15),24.0(C-2),20.2(C-14),13.0(C-13),12.8(C-21),11.1(C-22);HR MS:C22H34N2O5(M+Na)+计算值429.2360,测定值429.2356.
3a-6:黄色油状物;(c 1.0mg/mL,CH2Cl2).1H NMR(300MHz,CDCl3)δ:0.76(3H,d,J=7.5Hz,H-14),0.83(3H,d,J=6.0Hz,H-13),1.39(3H,s,H-15),1.15-1.99(10H,m,H-2,H-3,H-7~H-10),2.24-2.35(H,m,H-1),2.52-2.59(H,m,H-11),3.70-3.76(1H,m,H-16),4.28-4.43(3H,m,H-16 and H-17),4.73(1H,d,J=2.7Hz,H-12),4.91(1H,s,H-5),7.30(2H,t,J=2.4Hz,H-21 and H-22),7.42(1H,d,J=7.5Hz,H-23),7.80(1H,d,J=7.5Hz,H-20),7.95(1H,s,H-18).
3b-1:m.p.:109.3-110.2℃;(c 1.0mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.94(3H,d,J=6.9Hz,H-14),0.96(3H,d,J=5.6Hz,H-13),1.44(3H,s,H-15),1.17-2.22(12H,m,H-2,H-3,H-7~H-10 and H-17),2.37(1H,m,H-1),2.59-2.72(1H,m,H-11),3.28-3.36(1H,m,H-16),3.82-3.89(1H,m,H-16),4.23(2H,t,J=7.0Hz,H-18),4.78(1H,d,J=3.3Hz,H-12),5.40(1H,s,H-5),6.25(1H,s,H-20),7.37(1H,s,H-19),7.52(1H,s,H-21);13C NMR(75MHz,CDCl3)δ:139.31(C-21),129.09(C-20),105.25(C-19),104.06(C-4),102.01(C-12),87.84(C-5),81.00(C-6),64.96(C-16),52.47(C-1),49.00,44.29(C-7),37.38(C-11),36.33(C-10),34.54(C-3),30.82(C-17),30.49(C-9),26.13(C-8),24.62(C-15),24.46(C-2),20.33(C-14),13.09(C-13).HR MS:C21H32N2O5(M+Na)+计算值415.2203,测定值415.2202.
3b-2:黄色浆状物;(c 1.0mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.94-0.98(6H,m,H-13 and H-14),1.43(3H,s,H-15),1.25-2.06(12H,m,H-2,H-3,H-7~H-10 and H-17),2.33-2.42(1H,m,H-1),2.60-2.71(1H,m,H-11),3.33-3.40(1H,m,H-16),3.83-3.90(1H,m,H-16),4.04(2H,t,J=7.2Hz,H-18),4.78(1H,s,H-12),5.37(1H,s,H-5),6.91(1H,s,H-20),7.07(1H,s,H-19),7.47(1H,s,H-21);13C NMR(75MHz,CDCl3)δ:137.1(21),129.5(C-20),118.8(C-19),104.1(C-4),102.0(C-12),87.9(C-5),80.9(C-6),64.4(C-16),52.5(C-1),44.2(C-7),43.8(C-18),37.5(C-11),36.3(C-10),34.5(C-3),31.2(C-17),30.8(C-9),26.1(C-8),24.6(C-15),24.5(C-2),20.3(C-14),13.1(C-13).HR MS:C21H32N2O5[M+H]+计算值393.2384,测定值393.2386.
3b-3:黄色油状物;(c 1.0mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.91(3H,d,J=6.9Hz,H-14),0.95(3H,d,J=6.3Hz,H-13),1.45(3H,s,H-15),1.19-2.06(12H,m,H-2,H-3,H-7~H-10 and H-17),2.13(3H,s,H-22),2.32-2.43(1H,m,H-1),2.59-2.66(1H,m,H-11),3.40-3.52(4H,m,H-16 and H-18),4.69(1H,d,J=2.7Hz,H-12),5.39(1H,s,H-5);13C NMR(75MHz,CDCl3)δ:127.9(C-19),127.2(C-21),118.9(C-20),104.2(C-4),102.0(C-12),87.8(C-5),80.9(C-6),66.9(C-16),52.4(C-1),47.8(C-18),44.0(C-7),37.2(C-11),36.3(C-10),34.4(C-3),30.6(C-9),29.7(C-17),26.1(C-8),24.6(C-15),24.2(C-2),20.3(C-14),13.0(C-13),12.7(C-21).HR MS:C22H34N2O5[M+H]+计算值407.2540,测定值407.2542.
3b-4:黄色油状物;(c 1.7mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.85(3H,d,J=7.2Hz,H-14),0.94(3H,d,J=4.8Hz,H-13),1.43(3H,s,H-15),1.22-2.07(12H,m,H-2,H-3,H-7~H-10 and H-17),2.29-2.34(1H,m,H-1),2.41(3H,s,H-21),2.61-2.63(1H,m,H-11),3.58-3.63(1H,m,H-16),3.99-4.16(3H,m,H-16 and H-17),4.76(1H,d,J=3.3Hz,H-12),5.08(1H,s,H-5).6.88(1H,s,H-20),6.92(1H,s,H-19);13C NMR(75MHz,CDCl3)δ:126.7(C-20 and C-19),118.9(C-18),104.2(C-4),102.0(C-12),87.8(C-5),80.9(C-6),66.8(C-16),52.4(C-1),45.8(C-17),44.0(C-7),37.2(C-11),36.3(C-10),34.4(C-3),30.6(C-9),29.6(C-17),26.1(C-8),24.6(C-15),24.2(C-2),20.3(C-14),13.0(C-13),12.9(C-21).HR MS:C22H34N2O5[M+H]+计算值407.2540,测定值407.2537.
3b-5:黄色油状物;(c 2.5mg/mL,CHCl3).1H NMR(400MHz,CDCl3)δ:0.93(3H,d,J=7.4Hz,H-13),0.96(3H,d,J=7.4Hz,H-14),1.44(3H,s,H-15),1.37-2.12(12H,m,H-2,H-3,H-7~H-10 and H-17),2.21(3H,s,H-21),2.29(3H,s,H-22),2.39(1H,m,H-1),2.61-2.68(1H,m,H-11),3.36-3.41(1H,m,H-16),3.85-3.90(1H,m,H-16),4.03(2H,t,J=7.1Hz,H-18),4.80(1H,d,J=2.4Hz,H-12),5.45(1H,s,H-5),5.77(1H,s,H-19);13C NMR(75MHz,CDCl3)δ:147.18(C-21 and C-23),138.39(C-20),104.77(C-4),103.98(C-20),101.79(C-12),87.81(C-5),80.97(C-6),65.07(C-16),52.44(C-1),45.56(C-17),44.30(C-7),37.30(C-11),36.31(C-10),34.54(C-3),30.79(C-9),30.53(C-18),26.07(C-8),24.59(C-15),24.43(C-2),20.27(C-14),13.39(C-13),13.02(C-13),10.85(C-22 and C-23).HR MS:C23H36N2O5[M+Na]+计算值443.2516,测定值443.2518.
3b-6:黄色浆状物;(c 1.5mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.87(3H,d,J=6.9Hz,H-13),0.98(3H,d,J=7.5Hz,H-14),1.44(3H,s,H-15),1.26-2.16(12H,m,H-2,H-3,H-7~H-10 and H-17),2.33-2.43(1H,m,H-1),2.68-2.71(1H,m,H-11),3.39-3.47(1H,m,H-16),3.88-3.95(1H,m,H-16),4.30(2H,t,J=7.2Hz,H-18),4.82(1H,d,J=2.4Hz,H-12),5.40(1H,s,H-5),7.30-7.33(2H,m,H-22 and H-23),7.41(1H,d,J=4.8Hz,H-21),7.82-7.85(1H,m,H-24),7.98(1H,s,H-19);13C NMR(75MHz,CDCl3)δ:129.1(C-21 and C-24),116.1(C-22 and C-23),104.0(C-4),102.0(C-12),87.8(C-5),81.0(C-6),66.4(C-16),52.5(C-1),49.0(C-18),44.0(C-7),37.5(C-11),36.4(C-10),34.6(C-3),30.7(C-9),29.7(C-17),26.2(C-8),24.7(C-15),24.5(C-2),20.4(C-14),13.0(C-13).HRMS:C25H34N2O5[M+H]+计算值443.2541,测定值443.2538.
4a-1:m.p.:100.8-102.4℃;(c 1.0mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.74(3H,d,J=7.2Hz,H-14),0.93(3H,d,J=5.4Hz,H-13),1.42(3H,s,H-15),1.12-2.11(10H,m,H-2,H-3,H-7~H-10),2.34(1H,m,H-1),2.47-2.63(1H,m,H-11),3.89-3.96(1H,m,H-16),4.33-4.40(1H,m,H-16),4.64(2H,m,H-17),4.77(1H,s,H-12),5.21(1H,s,H-5),7.60(2H,s,H-18);13C NMR(75MHz,CDCl3)δ:133.96(C-18),103.97(C-4),102.10(C-12),87.79(C-5),80.92(C-6),66.12(C-16),54.73(C-17),52.37(C-1),44.12(C-7),37.13(C-11),36.29(C-10),34.47(C-3),30.58(C-9),26.08(C-8),24.58(C-15),23.93(C-2),20.31(C-14),12.67(C-13).HR MS:C19H29N3O5[M+Na]+计算值402.1999,测定值402.1997.
4a-2:m.p.:128.4-129.7℃;(c 1.0mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.81(3H,d,J=7.2Hz,H-14),0.95(3H,d,J=5.4Hz,H-13),1.42(3H,s,H-15),1.16-2.08(10H,m,H-2,H-3,H-7~H-10),2.35(1H,m,H-1),2.56-2.65(1H,m,H-11),3.77-3.84(1H,m,H-16),4.26-4.33(1H,m,H-16),4.52-4.60(1H,m,H-17),4.63-4.72(1H,m,H-17),4.78(1H,d,J=3.2Hz,H-12),5.16(1H,s,H-5),7.62(1H,s,H-18),7.72(1H,s,H-19);13C NMR(75MHz,CDCl3)δ:133.62(C-19),123.94(C-18),104.11(C-4),102.06(C-12),87.77(C-5),80.78(C-6),66.46(C-16),52.33(C-1),50.12(C-17),43.97(C-7),37.18(C-11),36.23(C-10),34.35(C-3),30.54(C-9),26.04(C-8),24.51(C-15),24.19(C-2),20.30(C-14),12.80(C-13).HR MS:C19H29N3O5[M+Na]+计算值402.1999,测定值402.1993.
4a-3:m.p.:93.5-95.2℃;(c 1.1mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.78(3H,d,J=7.2Hz,H-14),0.94(3H,d,J=4.5Hz,H-13),1.42(3H,s,H-15),1.19-2.08(10H,m,H-2,H-3,H-7~H-10),2.31-2.39(1H,m,H-1),2.58-2.62(1H,m,H-11),3.74-3.81(1H,m,H-16),4.09-4.17(1H,m,H-16),4.36-4.38(2H,m,H-17),4.78(1H,s,H-12),5.17(1H,s,H-5),7.96(1H,s,H-19),8.12(1H,s,H-18).
4a-4:m.p.:100.5~104.3℃;(c 3.0mg/mL,CH2Cl2).1H NMR(300MHz,CDCl3)δ:0.89(3H,d,J=7.4Hz,H-14),0.96(3H,d,J=5.6Hz,H-13),1.44(3H,s,H-15),1.27-2.10(10H,m,H-2,H-3,H-7~H-10),2.37(1H,td,J=14.2,3.7Hz,H-1),2.63-2.74(1H,m,H-11),3.61-3.70(1H,m,H-16),4.08-4.23(3H,m,H-16 and H-17),4.81(1H,d,J=3.5Hz,H-12),5.27(1H,s,H-5),7.80(1H,s,H-18);13C NMR(75MHz,CDCl3)δ:154.04,140.14,104.17,102.24,87.74,80.72,66.47,52.25,43.91,43.51,37.23,36.18,34.25,30.49,25.95,24.47,24.40,20.21,12.83.HR MS:C19H30N4O5[M+Na]+计算值417.2108,测定值417.2105.
4a-5:m.p.:97.3-98.5℃;(c 1.0mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.91(3H,d,J=7.4Hz,H-14),0.94(3H,d,J=6.3Hz,H-13),1.41(3H,s,H-15),1.21-2.09(10H,m,H-2,H-3,H-7~H-10),2.37(1H,td,J=14.1,3.6Hz,H-1),2.54-2.69(1H,m,H-11),3.23(2H,t,J=5.8Hz,H-17),3.82-3.66(1H,m,H-16),3.98-4.06(1H,m,H-16),4.83(1H,d,J=3.2Hz,H-12),5.06(2H,s,H-20),5.57(1H,s,H-5);13C NMR(75MHz,CDCl3)δ:163.32,148.19,104.35,101.96,88.16,81.26,67.28,52.40,44.26,37.28,36.30,34.52,32.05,30.87,25.94,24.52,24.33,20.33,12.93.HR MS:C19H30N4O5S[M-H]-计算值425.1864,测定值425.1866.
4a-6:m.p.:139.3-140.5℃;(c 0.8mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.63(3H,d,J=7.5Hz,H-13),0.85(3H,d,J=6.0Hz,H-14),1.39(3H,s,H-15),0.97-1.99(10H,m,H-2,H-3,H-7~H-10),2.23-2.34(1H,m,H-1),2.47-2.50(1H,m,H-11),3.88-3.93(1H,m,H-16),4.45-4.52(1H,m,H-16),4.73(1H,d,J=3.0Hz,H-12),4.80-4.93(3H,m,H-5 and H-17),7.37(1H,t,J=7.5Hz,H-21),7.49(1H,t,J=7.2Hz,H-20),7.57(1H,d,J=8.1Hz,H-19),7.86(2H,d,J=8.4Hz,H-22);13C NMR(75MHz,CDCl3)δ:145.8(C-23),133.5(C-18),127.1(C-21),123.8(C-20),119.8(C-22),109.6(C-19),104.0(C-4),102.0(C-12),87.6(C-5),80.7(C-6),66.1(C-16),52.2(C-1),48.0(C-17),43.9(C-7),36.9(C-11),36.2(C-10),34.3(C-3),30.5(C-9),26.0(C-8),24.4(C-15),24.0(C-2),20.2(C-14),12.6(C-13).HR MS:C23H31N3O5[M+Na]+计算值452.2156,测定值452.2151.
4a-7:m.p.:65.6-67.3℃;(c 1.0mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.73(3H,d,J=6.9Hz,H-13),0.87(3H,d,J=7.5Hz,H-14),1.42(3H,s,H-15),1.01-1.99(10H,m,H-2,H-3,H-7~H-10),2.25-2.33(1H,m,H-1),2.49-2.55(1H,m,H-11),4.00-4.04(1H,m,H-16),4.62-4.68(1H,m,H-16),4.81(1H,s,H-12),4.89-5.03(3H,m,H-5and H-17),7.38(1H,d,J=9.0Hz,H-20),7.86(2H,d,J=9.3Hz,H-19);13C NMR(75MHz,CDCl3)δ:143.5(C-18),126.2(C-20),117.9(C-19),103.9(C-4),101.7(C-12),87.7(C-5),80.8(C-6),65.5(C-16),56.5(C-17),52.2(C-1),44.0(C-7),36.7(C-11),36.3(C-10),34.3(C-3),30.6(C-9),26.1(C-8),24.5(C-15),23.9(C-2),20.1(C-14),12.7(C-13).HRMS:C23H31N3O5[M+Na]+计算值452.2156,测定值452.2159.
4a-8:m.p.:131.2-132.7℃;(c 1.0mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.91(3H,d,J=7.2Hz,H-14),0.95(3H,d,J=6.0Hz,H-13),1.44(3H,s,H-15),1.26-2.06(10H,m,H-2,H-3,H-7~H-10),2.32-2.43(H,m,H-1),2.65-2.72(H,m,H-11),3.83~3.90(1H,m,H-16),4.22-4.29(1H,m,H-16),4.75(2H,t,J=3.6Hz,H-17),4.87(1H,s,H-12),5.45(1H,s,H-5),7.40(1H,t,J=7.5Hz,H-21),7.52(1H,t,J=7.5Hz,H-20),7.62(1H,d,J=8.4Hz,H-22),8.03(1H,d,J=8.4Hz,H-19);13C NMR(75MHz,CDCl3)δ:143.5(C-23),127.9(C-18),127.4(C-19),124.6(C-21),120.3(C-20),108.6(C-22),104.1(C-4),102.3(C-12),88.0(C-5),81.0(C-6),79.7(C-17),65.5(C-16),52.5(C-1),44.3(C-7),37.3(C-11),36.3(C-10),34.5(C-3),30.7(C-9),26.1(C-8),24.6(C-15),24.3(C-2),20.3(C-14),12.9(C-13).HR MS:C23H31N3O6[M+Na]+计算值468.2105,测定值468.2098.
4b-1:m.p.:100.8-102.4℃;(c 1.0mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.94(3H,d,J=7.2Hz,H-14).,0.96(3H,d,J=6.2Hz,H-13),1.43(3H,s,H-15),1.17-2.10(10H,m,H-2,H-3,H-7~H-10),2.16-2.28(2H,m,H-17),2.37(1H,m,H-1),2.56-2.72(1H,m,H-11),3.26-3.43(1H,m,H-16),3.81-3.88(1H,m,H-16),4.54(2H,m,H-18),4.79(1H,d,J=3.2Hz,H-12),5.44(1H,s,H-5),7.59(2H,s,H-18);13C NMR(75MHz,CDCl3)δ:133.98(C-19),104.00(C-4),102.09(C-12),87.85(C-5),81.05(C-6),64.78(C-16),52.51(C-17),51.86(C-1),44.34(C-7),37.29(C-11),36.36(C-10),34.59(C-3),30.84(C-9),29.88(C-18),26.15(C-8),24.64(C-15),24.43(C-2),20.35(C-14),13.01(C-13).HR MS:C20H31N3O5[M+Na]+计算值416.2156,测定值416.2155.
4b-2:m.p.:100.8-102.4℃;(c 3.5mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.94(3H,d,J=7.2Hz,H-14),0.96(3H,d,J=6.1Hz,H-13),1.43(3H,s,H-15),1.21-2.25(12H,m,H-2,H-3,H-7~H-10 and H-17),2.28-2.43(1H,m,H-1),2.65(1H,m,H-11),3.31-3.43(1H,m,H-16),3.83-3.94(1H,m,H-16),4.46-4.52(2H,m,H-17),4.78(1H,d,J=3.2Hz,H-12),5.40(1H,s,H-5),7.56(1H,s,H-18),7.72(1H,s,H-19);13C NMR(75MHz,CDCl3)δ:133.77(C-20),123.48(C-19),104.10(C-4),102.04(C-12),87.84(C-5),80.94(C-6),64.52(C-16),52.41(C-1),47.19(C-17),44.18(C-7),37.34(C-11),36.27(C-10),34.46(C-3),30.75(C-9),30.41(C-18),26.08(C-8),24.56(C-15),24.45(C-2),20.31(C-14),13.04(C-13).HR MS:C20H31N3O5[M+Na]+计算值416.2156,测定值416.2157.
4b-3:黄色油状物;(c 1.7mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.92-0.97(6H,m,H-14 and H-13),1.43(3H,s,H-15),1.19-2.08(10H,m,H-2,H-3,H-7~H-10),2.16-2.19(2H,m,H-17),2.32-2.36(1H,m,H-1),2.64-2.67(1H,m,H-11),3.32-3.40(1H,m,H-16),3.84-3.91(1H,m,H-16),4.28(2H,t,J=6.9Hz,H-18),4.77(1H,s,H-12),5.39(1H,s,H-5),7.96(1H,s,H-20),8.09(1H,s,H-19);13C NMR(75MHz,CDCl3)δ:151.9(C-20),143.0(19),104.1(C-4),102.0(C-12),87.8(C-5),80.9(C-6),64.5(C-16),52.4(C-1),46.7(C-18),44.2(C-7),37.4(C-11),36.3(C-10),34.5(C-3),30.7(C-9),29.9(C-18),26.1(C-8),24.6(C-15),24.5(C-2),20.3(C-14),13.0(C-13).HR MS:C20H31N3O5[M+Na]+计算值416.2156,测定值416.2155.
4b-4:黄色浆状物;(c 1.5mg/mL,CH2Cl2).1H NMR(300MHz,CDCl3)δ:0.93(3H,d,J=7.4Hz,H-14),0.96(3H,d,J=5.1Hz,H-13),1.43(3H,s,H-15),1.25-2.15(12H,m,H-2,H-3,H-7~H-10 and H-17),2.37(1H,td,J=14.0,3.8Hz,H-1),2.62-2.71(1H,m,H-11),3.55-3.62(1H,m,H-16),3.84-3.92(1H,m,H-16),3.97-4.08(2H,m,H-18),4.81(1H,d,J=3.3Hz,H-12),5.44(1H,s,H-5),7.65(1H,s,H-19);13C NMR(75MHz,CDCl3)δ:153.99,142.09,104.21,101.97,87.84,80.88,64.67,52.43,46.28,44.22,37.41,36.26,34.45,30.77,29.32,26.04,24.59,24.46,20.28,13.06.HR MS:C20H32N4O5[M+Na]+计算值431.2265,测定值431.2266.
4b-5:黄色浆状物;(c 2.9mg/mL,CH2Cl2).1H NMR(300MHz,CDCl3)δ:0.91(3H,d,J=7.4Hz,H-14),0.94(3H,d,J=6.3Hz,H-13),1.41(3H,s,H-15),1.21-2.09(12H,m,H-2,H-3,H-7~H-10 and H-17),2.37(1H,td,J=14.1,3.6Hz,H-1),2.54-2.69(1H,m,H-11),3.23(2H,t,J=5.8Hz,H-18),3.66-3.82(1H,m,H-16),3.98-4.06(1H,m,H-16),4.80(1H,d,J=3.2Hz,H-12),5.44(2H,s,H-21),5.57(1H,s,H-5);13C NMR(75MHz,CDCl3)δ:163.55,148.20,104.21,101.97,87.93,80.93,64.67,52.43,44.22,37.40,36.31,34.50,30.87,29.65,26.11,24.59,24.46,20.31,13.06.HR MS:C20H32N4O5S[M+Na]+计算值463.1986,测定值463.1986.
4b-6:黄色浆状物;(c 1.3mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.94(3H,d,J=7.5Hz,H-13),0.97(3H,d,J=6.9Hz,H-14),1.42(3H,s,H-15),1.21-2.06(10H,m,H-2,H-3,H-7~H-10),2.31-2.44(3H,m,H-1 and H-17),2.60-2.70(1H,m,H-11),3.37-3.45(1H,m,H-16),3.86-3.93(1H,m,H-16),4.79-4.87(3H,m,H-12 and H-18),5.50(1H,s,H-5),7.37-7.41(2H,m,H-21),7.84-7.88(2H,m,H-20);13C NMR(75MHz,CDCl3)δ:144.3(C-19),126.3(C-21),117.9(C-20),104.0(C-4),102.1(C-12),87.9(C-5),81.1(C-6),64.8(C-16),53.7(C-18),52.6(C-1),44.4(C-7),37.3(C-11),36.4(C-10),34.6(C-3),30.7(C-9),26.2(C-8),24.7(C-15),24.5(C-2),22.7(C-17),20.4(C-14),13.0(C-13).HRMS:C24H33N3O5[M+Na]+计算值466.2312,测定值466.2309.
4b-7:黄色浆状物;(c 1.0mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.95-0.98(6H,m,H-13 and H-14),1.43(3H,s,H-15),1.26-2.09(10H,m,H-2,H-3,H-7~H-10),2.28-2.42(3H,m,H-1 and H-17),2.65-2.70(1H,m,H-11),3.41-3.48(1H,m,H-16),3.88-3.95(1H,m,H-16),4.72-4.78(2H,m,H-18),4.81(1H,d,J=3.3Hz,H-12),5.44(3H,s,H-5),7.38(1H,t,J=7.5Hz,H-21),7.46-7.55(2H,m,H-22 and H-23),8.08(1H,d,J=7.8Hz,H-20);13C NMR(75MHz,CDCl3)δ:145.9(C-19),133.0(C-24),127.3(C-21),123.9(C-22),120.0(C-20),109.2(C-23),104.2(C-4),102.1(C-12),87.9(C-5),81.0(C-6),64.8(C-16),52.5(C-1),45.3(C-18),44.3(C-7),37.4(C-11),36.3(C-10),34.6(C-3),30.6(C-9),29.9(C-17),26.1(C-8),24.6(C-15),24.5(C-2),20.3(C-14),13.1(C-13).HR MS:C24H33N3O5[M+Na]+计算值466.2312,测定值466.2310.
4b-8:m.p.:106.3-108.1℃;(c 1.0mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.88(3H,d,J=9.3Hz,H-14),0.93(3H,d,J=7.2Hz,H-13),1.45(3H,s,H-15),1.22-2.03(10H,m,H-2,H-3,H-7~H-10),2.13-2.17(2H,m,H-17),2.32-2.42(H,m,H-1),2.64-2.71(H,m,H-11),3.62-3.67(1H,m,H-16),4.10~4.18(1H,m,H-16),4.66(2H,t,J=6.0Hz,H-18),4.85(1H,s,H-12),5.43(1H,s,H-5),7.40(1H,t,J=7.2Hz,H-21),7.52(1H,t,J=7.2Hz,H-22),7.59(1H,d,J=7.8Hz,H-23),8.02(1H,d,J=8.7Hz,H-20);13C NMR(75MHz,CDCl3)δ:143.5(C-24),127.9(C-19),127.3(C-20),124.5(C-22),120.3(C-21),108.5(C-23),104.1(C-4),102.1(C-12),87.9(C-5),81.0(C-6),77.4(C-18),64.1(C-16),52.5(C-1),44.3(C-7),37.3(C-11),36.4(C-10),34.5(C-3),30.8(C-9),28.6(C-17),26.1(C-8),24.6(C-15),24.5(C-2),20.3(C-14),13.0(C-13).HR MS:C24H33N3O6[M+Na]+计算值482.2262,测定值482.2260.
5a-1:m.p.:135.2-136.5℃;(c 1.0mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.72(3H,d,J=7.8Hz,H-13),0.95(3H,d,J=5.1Hz,H-14),1.43(3H,s,H-15),1.25-2.06(10H,m,H-2,H-3,H-7~H-10),2.31-2.40(1H,m,H-1),2.63-2.65(1H,m,H-11),3.80-3.86(1H,m,H-16),4.28-4.35(1H,m,H-16),4.63(2H,t,J=4.8Hz,H-18),4.79(1H,s,H-12),5.19(1H,s,H-5),8.64(1H,s,H-18);13C NMR(75MHz,CDCl3)δ:143.0(C-18),104.2(C-4),102.3(C-12),87.9(C-5),80.7(C-6),65.7(C-16),52.3(C-1),48.4(C-17),43.9(C-7),37.3(C-11),36.3(C-10),34.3(C-3),30.5(C-9),26.0(C-8),24.5(C-15),24.3(C-2),20.3(C-14),12.8(C-13).HR MS:C18H28N4O5[M+Na]+计算值403.1952,测定值403.1951.
5a-2:m.p.:123.2-124.6℃;(c 1.0mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.72(3H,d,J=7.5Hz,H-13),0.95(3H,d,J=4.8Hz,H-14),1.43(3H,s,H-15),1.24-2.05(10H,m,H-2,H-3,H-7~H-10),2.31-2.39(1H,m,H-1),2.54-2.61(1H,m,H-11),3.96-4.03(1H,m,H-16),4.38-4.45(1H,m,H-16),4.78(1H,s,H-12),4.87(2H,t,J=4.8Hz,H-17),5.22(1H,s,H-5),8.52(1H,s,H-18);13C NMR(75MHz,CDCl3)δ:152.8(C-18),104.1(C-4),102.2(C-12),87.9(C-5),80.9(C-6),65.5(C-16),53.1(C-17),52.4(C-1),44.1(C-7),37.3(C-11),36.3(C-10),34.4(C-3),30.6(C-9),26.1(C-8),24.6(C-15),24.0(C-2),20.3(C-14),12.6(C-13).HR MS:C18H28N4O5[M+Na]+计算值403.1952,测定值403.1949.
5a-3:m.p.:117.4-119.1℃;(c 1.1mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.93(3H,d,J=7.5Hz,H-13),0.95(3H,d,J=5.7Hz,H-13),1.43(3H,s,H-15),1.26-2.07(10H,m,H-2,H-3,H-7~H-10),2.32-2.42(1H,m,H-1),2.56(1H,s,H-20),2.62-2.73(1H,m,H-11),3.41-3.49(1H,m,H-16),3.87-3.94(1H,m,H-16),4.33-4.38(2H,m,H-18),4.80(1H,s,H-12),5.40(1H,s,H-5);13C NMR(75MHz,CDCl3)δ:162.75,103.96,102.03,87.79,80.89,64.22,52.46,49.83,44.24,37.22,36.29,34.53,30.73,26.03,24.58,24.39,20.27,12.90,10.76.HR MS:C19H30N4O5[M+Na]+计算值417.2108,测定值417.2106.
5a-4:m.p.:114.2-115.7℃;(c 1.0mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.75(3H,d,J=7.2Hz,H-14),0.94(3H,d,J=4.5Hz,H-13),1.42(3H,s,H-15),1.21-2.04(10H,m,H-2,H-3,H-7~H-10),2.29-2.38(1H,m,H-1),2.57(3H,s,H-19),2.58-2.61(1H,m,H-11),3.80-3.87(1H,m,H-16),4.34-4.37(1H,m,H-16),4.46-4.48(2H,m,H-17),4.76(1H,s,H-12),5.11(1H,s,H-5);13C NMR(75MHz,CDCl3)δ:152.1(C-18),104.2(C-4),102.3(C-12),87.7(C-5),80.7(C-6),65.8(C-16),52.3(C-1),47.0(C-17),43.9(C-7),37.2(C-11),36.2(C-10),34.3(C-3),30.5(C-9),26.0(C-8),24.5(C-15),24.2(C-2),20.3(C-14),13.0(C-13),8.9(C-19).HR MS:C19H30N4O5[M+Na]+计算值417.2108,测定值417.2106.
5a-5:m.p.:121.7-123.3℃;(c 1.0mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.90(3H,d,J=7.5Hz,H-14),0.96(3H,d,J=6.3Hz,H-13),1.43(3H,s,H-15),1.21-2.04(10H,m,H-2,H-3,H-7~H-10),2.32-2.43(H,m,H-1),2.61-2.66(H,m,H-11),3.63(2H,t,J=5.7Hz,H-17),3.75-3.83(1H,m,H-16),3.93(3H,s,H-19),4.12-4.19(H,m,H-16),4.83(1H,d,J=2.7Hz,H-12),5.43(1H,s,H-5);13C NMR(75MHz,CDCl3)δ:154.0(C-18),104.1(C-4),102.2(C-12),87.9(C-5),80.9(C-6),66.5(C-16),52.4(C-1),44.2(C-7),37.4(C-11),36.3(C-10),34.5(C-3),33.6(C-17),33.4(C-19),30.7(C-9),26.1(C-8),24.6(C-15),24.3(C-2),20.3(C-14),12.9(C-13).HR MS:C19H30N4O5S[M+Na]+计算值449.1829,测定值449.1821.
5a-6:m.p.:129.8-130.8℃;(c 1.0mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.87(3H,d,J=6.9Hz,H-13),0.98(3H,d,J=7.5Hz,H-14),1.44(3H,s,H-15),1.26-2.12(10H,m,H-2,H-3,H-7~H-10),2.33-2.43(1H,m,H-1),2.68-2.71(1H,m,H-11),3.94-4.00(1H,m,H-16),4.52-4.59(1H,m,H-16),4.78-4.82(1H,m,H-17),4.83(1H,d,J=2.7Hz,H-12),4.91-4.99(1H,m,H-17),5.15(1H,s,H-5),7.47-7.50(3H,m,H-21 and H-22),8.17(1H,d,J=5.4Hz,H-20);13C NMR(75MHz,CDCl3)δ:165.1(C-18),130.3(C-22),128.8(C-21),127.3(C-19),126.7(C-20),104.0(C-4),102.0(C-12),87.8(C-5),80.8(C-6),64.9(C-16),52.1(C-1),52.3(C-17),44.0(C-7),37.1(C-11),36.3(C-10),34.2(C-3),30.6(C-9),26.1(C-8),24.4(C-15),24.0(C-2),20.0(C-14),12.87(C-13).HR MS:C24H32N4O5[M+Na]+计算值479.2265,测定值479.2268.
5b-1:黄色油状物;(c 1.0mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.94(3H,d,J=7.8Hz,H-13),0.97(3H,d,J=6.6Hz,H-14),1.44(3H,s,H-15),1.26-2.06(10H,m,H-2,H-3,H-7~H-10),2.22-2.28(2H,m,H-17),2.33-2.42(1H,m,H-1),2.63-2.70(1H,m,H-11),3.39-3.46(1H,m,H-16),3.87-3.94(1H,m,H-16),4.54(2H,t,J=6.9Hz,H-18),4.79(H,s,H-12),5.39(1H,s,H-5),8.62(1H,s,H-19);13C NMR(75MHz,CDCl3)δ:142.6(C-19),104.2(C-4),102.2(C-12),87.9(C-5),80.9(C-6),64.3(C-16),52.4(C-1),45.5(C-18),44.2(C-7),37.4(C-11),36.3(C-10),34.5(C-3),30.7(C-9),30.0(C-17),26.1(C-8),24.6(C-15),24.5(C-2),20.3(C-14),13.0(C-13).HR MS:C19H30N4O5[M+Na]+计算值417.2108,测定值417.2111.
5b-2:黄色油状物;(c 1.0mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.93-0.98(6H,m,H-13 and H-14),1.43(3H,s,H-15),1.26-2.06(10H,m,H-2,H-3,H-7~H-10),2.28-2.32(2H,m,H-17),2.37-2.43(1H,m,H-1),2.61-2.68(1H,m,H-11),3.35-3.42(1H,m,H-16),3.86-3.92(1H,m,H-16),4.74-4.79(3H,m,H-12 and H-18),5.41(1H,s,H-5),8.51(1H,s,H-19);13C NMR(75MHz,CDCl3)δ:152.8(C-19),104.1(C-4),102.2(C-12),87.9(C-5),81.0(C-6),64.4(C-16),52.5(C-1),50.2(C-18),44.3(C-7),37.4(C-11),36.4(C-10),34.6(C-3),30.8(C-9),29.5(C-17),26.1(C-8),24.6(C-15),24.5(C-2),20.3(C-14),13.0(C-13).HR MS:C19H30N4O5[M+Na]+计算值417.2108,测定值417.2114.
5b-3:黄色油状物;(c 1.0mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.93(3H,d,J=7.5Hz,H-13),0.95(3H,d,J=5.7Hz,H-13),1.43(3H,s,H-15),1.26-2.07(10H,m,H-2,H-3,H-7~H-10),2.16-2.22(2H,m,H-17),2.32-2.42(1H,m,H-1),2.56(1H,s,H-20),2.62-2.73(1H,m,H-11),3.41-3.49(1H,m,H-16),3.87-3.94(1H,m,H-16),4.33-4.38(2H,m,H-18),4.80(1H,s,H-12),5.40(1H,s,H-5);13C NMR(75MHz,CDCl3)δ:162.75(C-19),103.96(C-4),102.03(C-12),87.79(C-5),80.89(C-6),64.22(C-16),52.46(C-1),49.83(C-18),44.24(C-7),37.22(C-11),36.29(C-10),34.53(C-3),30.73(C-9),29.38(C-17),26.03(C-8),24.58(C-15),24.39(C-2),20.27(C-14),12.90(C-13),10.76(C-20).HRMS:C20H32N4O5[M+Na]+计算值431.2265,测定值431.2272.
5b-4:黄色油状物;(c 1.0mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.93(3H,d,J=7.5Hz,H-13),0.97(3H,d,J=6.1Hz,H-13),1.44(3H,s,H-15),1.19-2.10(10H,m,H-2,H-3,H-7~H-10),2.14-2.27(2H,m,H-17),2.32-2.47(1H,m,H-1),2.57(3H,s,H-20),2.64-2.73(1H,m,H-11),3.39-3.59(1H,m,H-16),3.88-3.95(1H,m,H-16),4.33-4.43(2H,m,H-18),4.81(1H,d,J=3.3Hz,H-12),5.41(1H,s,H-5);13C NMR(75MHz,CDCl3)δ:104.16(C-4),102.01(C-12),87.87(C-5),80.86(C-6),64.31(C-16),52.42(C-1),44.27(C-18),44.17(C-7),37.41(C-11),36.30(C-10),34.48(C-3),30.71(C-9),29.72(C-17),26.04(C-8),24.59(C-15),24.48(C-2),20.26(C-14),12.99(C-13),8.70(C-20).HR MS:C20H32N4O5[M+Na]+计算值431.2265,测定值431.2273.
5b-5:黄色浆状物;(c 1.0mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.91(3H,d,J=7.5Hz,H-13),0.95(3H,d,J=6.3Hz,H-13),1.43(3H,s,H-15),1.25-2.06(12H,m,H-2,H-3,H-7~H-10 and H-17),2.32-2.41(1H,m,H-1),2.62-2.67(1H,m,H-11),3.42-3.55(3H,m,H-16 and H-18),3.92(1H,s,H-20),3.97-4.04(1H,m,H-16),4.80(1H,d,J=3.0Hz,H-12),5.39(1H,s,H-5);13C NMR(75MHz,CDCl3)δ:154.2(C-19),104.1(C-4),102.1(C-12),87.9(C-5),81.0(C-6),66.0(C-16),52.4(C-1),44.2(C-7),37.4(C-11),36.3(C-10),34.5(C-3),33.3(C-18),30.8(C-9),30.3(C-17),29.1(C-20),26.1(C-8),24.6(C-15),24.5(C-2),20.3(C-14),13.0(C-13).HR MS:C20H32N4O5S[M+Na]+计算值463.1986,测定值463.1985.
5b-6:白色浆状物;(c 1.0mg/mL,CHCl3).1H NMR(300MHz,CDCl3)δ:0.87(3H,d,J=7.2Hz,H-13).0.96(3H,d,J=6.7Hz,H-14),1.43(3H,s,H-15),1.12-2.11(10H,m,H-2,H-3,H-7~H-10),2.31-2.37(3H,m,H-1,H-17),2.62-2.72(1H,m,H-11),3.36-3.54(1H,m,H-16),3.97-4.01(1H,m,H-16),4.73-4.81(2H,m,H-18),4.82(1H,d,J=2.7Hz,H-12),5.46(1H,s,H-5),7.42-7.58(3H,m,H-22 and H-23),8.27-8.07(2H,d,J=5.4Hz,H-21);13C NMR(75MHz,CDCl3)δ:165.11(C-19),130.21(C-23),128.80(C-22),127.40(C-20),126.81(C-21),104.07(C-4),102.21(C-12),87.87(C-5),80.98(C-6),64.51(C-16),52.52(C-1),50.28(C-17),44.31(C-7),37.37(C-11),36.37(C-10),34.55(C-3),30.82(C-9),29.67(C-18),26.12(C-8),24.61(C-15),24.53(C-2),20.31(C-14),12.99(C-13).HR MS:C25H34N4O5[M+Na]+计算值493.2427,测定值493.2421.
实施例3、DHA胺类衍生物和DHA唑类衍生物的抗疟活性测试
DHA胺类衍生物和DHA唑类衍生物的抗疟活性委托美国礼来公司Open InnovationDrug Discovery(OIDD)program进行测试,包括目标化合物对恶性疟原虫(Plasmodiumfalciparum)DD2株的抑制率、对人肝癌细胞HepG2中伯氏疟原虫(P.berghei)红外期(EEF)的抑制率以及对人肝癌细胞HepG2的毒性,首先进行单浓度初筛(Primary SP),然后对初步筛选出的潜力分子进行多浓度测试(Primary CRC)。结果见表5,6。
表5 DHA胺类衍生物的抗疟活性测试结果
从表5看出,测试的18个DHA胺类衍生物对恶性疟原虫有很强的抑制活性(抑制率85%-121%),其中化合物1e在2μM测试浓度下的抑制率达到106%,化合物1c、2a-2、2b-2在12.5μM测试浓度下的抑制率超过100%;DHA胺类衍生物对伯氏疟原虫红外期的抑制活性相对低一些(13%-82%),其中化合物1h、2a-1、2a-5、2b-1、2b-4、2b-5的抑制率在65%-82%;DHA胺类衍生物的细胞毒性很低(抑制率几乎都在±20%)。整体来看,DHA胺类衍生物具有高活性、低毒性的特点。
表6 DHA唑类衍生物的抗疟活性测试结果
从表6可以看出,测试的40个DHA唑类衍生物中有30个化合物在测试浓度下对恶性疟原虫的抑制率超过90%;DHA唑类衍生物对伯氏疟原虫红外期的抑制活性相对低一些,化合物3a-1,3a-3,3a-6,3b-1,4a-3,4b-1,5b-1,5b-2,5b-5,5b-6的抑制率在60%-88%之间;化合物3a-3,3a-6,3b-1,5b-1,5b-6对恶性疟原虫和伯氏疟原虫红外期的抑制活性都很强;特别地,几乎所有目标化合物都表现出较低的细胞毒性,抑制率在-5%~20%。整体来看,DHA唑类衍生物对疟原虫具有非常强的抑制作用,可作为药物进一步开发。
最后说明的是,以上优选实施例仅用以说明本发明的技术方案而非限制,尽管通过上述优选实施例已经对本发明进行了详细的描述,但本领域技术人员应当理解,可以在形式上和细节上对其作出各种各样的改变,而不偏离本发明权利要求书所限定的范围。
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