CN110447414A - A method of improving Momordica grosvenori mogroside V content - Google Patents
A method of improving Momordica grosvenori mogroside V content Download PDFInfo
- Publication number
- CN110447414A CN110447414A CN201910776404.2A CN201910776404A CN110447414A CN 110447414 A CN110447414 A CN 110447414A CN 201910776404 A CN201910776404 A CN 201910776404A CN 110447414 A CN110447414 A CN 110447414A
- Authority
- CN
- China
- Prior art keywords
- inducer
- momordica grosvenori
- content
- methyl jasmonate
- mogroside
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Botany (AREA)
- Medicines Containing Plant Substances (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
A method of Momordica grosvenori mogroside V content being improved, the method handles Lo Han Guo fruit using inducer before picking, and the inducer is trehalose, methyl jasmonate or sucrose, methyl jasmonate.The present invention directly acts on the immature fruit of Siraitia grosvenorii by Momordica grosvenori mogroside V inducer, it can promote the generation of momordica grosvenori alcohol and its hypo-glycosylated sweet tea glucoside, and conversion and accumulation to beet aglucone V, the functioning efficiency of inducer is promoted, treated, and sweet Momordica grosvenori salidroside content is more than the 50~100% of control；The present invention is handled without the plant of section between the tissue-cultured seedling stringent to environmental requirement and other growth and development simultaneously, while reducing the frequency of fruit processing, and processing method is simple and effective, is suitble to industrialized production.
Description
Technical field
The present invention relates to field of biotechnology, in particular to a kind of method for improving Momordica grosvenori mogroside V content.
Background technique
Siraitia grosvenorii is the traditional medical and edible dual purpose plant in China, containing a variety of active ingredients, the nonsugar triterpene of strong sweet taste
Constituents mogroside is its main effective component, and wherein the content of momordica glycoside V is the abundantest.And because of sweet Momordica grosvenori
Glycosides V sugariness is high, and heat is low, lighter color, and water-soluble and stability is good, edible safety, and 1996, it was food that China, which ratifies the product,
Additive can be used as sweetener and partly or entirely replace sucrose and be widely used in various types of beverages food, especially as obesity
And the alternative sugar of diabetic.It grinds to make internal disorder or usurp and shows that mogroside is used as functional efficient natural sweetener to have and widely answers
Use prospect.
Mogroside in Siraitia grosvenorii belongs to cucurbitane triterpene glycosides compound, mainly includes Momordia grosvenori aglycone IV, arhat
Fruit glycosides V and Simon glycosides I etc., wherein accounting for about fresh fruit with Mogroside V content highest and weighing 0.4~0.5%.50% He in the market
90% content sweet tea glycosides V extract price is up to 4000 yuan/kg and 20000 yuan/kg respectively, therefore in food, health care product, daily strong
Health articles field can not still be competed with sucrose.
There are two the current method for improving momordica glycoside V content is main: first, the method for passing through extraction purification promotes sweet tea
The content of glycosides V, such as Chinese patent CN106967142B " a kind of while extracting momordica glycoside V, VI and 11-O base glycosides V method "
Deng；Second, the content of sweet tea glycosides V in Siraitia grosvenorii is promoted by using emerging beet aglucone V inducer, such as:
Chinese patent CN106718920B discloses a kind of method for improving momordica glycoside V content, comprising the following steps:
Step 1: methyl jasmonate is dissolved in the ethanol water that volume fraction is 20%, it is configured to the jasmine that concentration is 50mmol/L
The ethanol mother liquor of jasmine acid methyl esters；Methyl jasmonate is dissolved in ethyl alcohol, the jasmonic first that concentration is 50~400 μm of ol/L is configured to
Ester solution；Step 2: Luohanguo With Plantlets of Tissue Culture is seeded in solid medium, wherein when making solid medium by jasmine
The ethanol mother liquor of sour methyl esters is added in solid medium, makes 50~400 μm of ol/L of ultimate density of methyl jasmonate；Step
Three, by tissue culture transplantation of seedlings to field, 50~60d after Pollination of Luohanguo, methyl jasmonate of daily early, middle and late each sprinkling
Solution continuously sprays 10d.
Chinese patent CN108277197A discloses mogroside Ⅴ content in a kind of raising Siraitia grosvenorii suspension cell
Method, the method by adding the ferment of 10~200mg/L at the 5th~11 day of the Siraitia grosvenorii suspension cell culture period respectively
The methyl jasmonate of female inducer and 50~260mg/L, to improve the synthesis rate and yield of mogroside Ⅴ.
But the use of above-mentioned inducer, can not be direct there are certain defect, methyl jasmonate treatment seedling and plant
Lo Han Guo fruit is acted on, the functioning efficiency of inducer is not high；Meanwhile the inducer can improve simultaneously containing for sweet tea glucoside II or VI
Amount, effect target be not single-minded.
Summary of the invention
It is an object of the invention to overcoming the deficiency of the above-mentioned prior art, provides and a kind of improve Momordica grosvenori mogroside V content
Method significantly improves the synthesis and accumulation of beet aglucone V in Lo Han Guo fruit maturation.
The purpose of the present invention is what is realized by following proposal:
The present invention provides a kind of method for improving Momordica grosvenori mogroside V content, and the method uses inducer before picking
Siraitia grosvenorii children okra fruit reality is handled, the inducer is trehalose, methyl jasmonate or sucrose, methyl jasmonate.
Preferably, the concentration of the inducer is 100~700mM of trehalose, 5~20mM of methyl jasmonate；
Preferably, the concentration of the inducer is 100~700mM of sucrose, 5~20mM of methyl jasmonate；
Preferably, the preparation method of the inducer is as follows: 3.5~4.8 grams of MS powder being dissolved in 1000 milliliters of water, are prepared
MS culture solution prepares the beet aglucone V inducer containing trehalose, methyl jasmonate or sucrose, methyl jasmonate with MS culture solution；
Preferably, the mode that the inducer is handled is for smearing or by spraying；
Preferably, the time of the inducer processing is to start to process within the 5th~15 day after pollinating；
Preferably, the time of the inducer processing is the 5th day to the 30th day after pollination, and the total degree of the processing is 2
~6 times；
Preferably, the time of the picking is the 70th~90 day after pollination.
The present invention overcomes following technical problems:
The present invention is directly made by applying trehalose, methyl jasmonate or sucrose, methyl jasmonate when planting Siraitia grosvenorii
For Lo Han Guo fruit, to induce momordica glycoside V route of synthesis key enzyme (such as cucurbit dienol synzyme and cytochromes
P450 enzyme etc.) gene high expression, improve the synthesis of cucurbit dienol, promote the glycosylation of cucurbit dienol and hypo-glycosylated
Conversion of the sweet tea glucoside (such as sweet tea glucoside II) to beet aglucone V, to quickly improve the content of Momordica grosvenori mogroside V in a short time.The present invention will
Inducer directly acts on Lo Han Guo fruit, and product will not be made to generate the substance being harmful to the human body, and the concentration of effect is in
Safely controllable range.
Beneficial effects of the present invention:
1, the present invention is handled without the tissue-cultured seedling stringent to environmental requirement, while reducing the frequency of fruit processing, place
Reason method is simple and effective, is suitble to industrialized production.
2, inducer of the invention does not need to act on seedling and plant, directly acts on Lo Han Guo fruit, promotes induction
The functioning efficiency of agent, treated, and sweet Momordica grosvenori salidroside content is more than the 50~100% of control.
3, inducer targeting target of the present invention is single-minded, can promote other hypo-glycosylated sweet tea glucosides (such as sweet tea glucoside II, sweet tea glucoside IV)
Into high-glycosylation Siraitia grosvenorii, the accumulation conversion of beet aglucone V, only promotes the content of beet aglucone V, will not promote sweet tea glucoside II or VI simultaneously
Content.
Detailed description of the invention
Fig. 1 beet aglucone V standard items HPLC figure.
The analysis HPLC of Fig. 2 control sample sweet tea salidroside content schemes.
Fig. 3 trehalose handles the analysis HPLC figure of sample sweet tea salidroside content.
Fig. 4 trehalose/methyl jasmonate treatment sample sweet tea salidroside content analysis HPLC figure.
The analysis HPLC of Fig. 5 saccharose treatment sample sweet tea salidroside content schemes.
Fig. 6 sucrose/methyl jasmonate treatment sample sweet tea salidroside content analysis HPLC figure.
Fig. 7 sucrose/influence of the methyl jasmonate treatment to Momordica grosvenori mogroside V content.
Wherein, SUC500 is that sucrose is used alone, and SUC500/MJ10 is that sucrose is used in combination with methyl jasmonate.The sea Fig. 8
Algae sugar/influence of the methyl jasmonate treatment to Momordica grosvenori mogroside V content.
Wherein, Tre500 is that trehalose is used alone, and Tre500/MJ10 is that trehalose is used in combination with methyl jasmonate.
Specific embodiment
Combined with specific embodiments below the present invention is made further to explain.
Embodiment one
The beet aglucone V inducer of 500mM containing trehalose, methyl jasmonate 10mM are prepared using conventional MS culture solution.Picking
Preceding to handle Lo Han Guo fruit using inducer, for processing mode using smearing or by spraying, number of processing is 4 times；Fruit maturation is adopted
It receives, momordica glycoside V content detection is carried out using HPLC method.
Embodiment two
The beet aglucone V inducer of 500mM containing sucrose, methyl jasmonate 10mM are prepared using conventional MS culture solution.Before picking
Lo Han Guo fruit is handled using inducer, for processing mode using smearing or by spraying, number of processing is 4 times；The harvesting of fruit maturation,
Momordica glycoside V content detection is carried out using HPLC method.
Embodiment three
One, the preparation of Momordica grosvenori mogroside V inducer:
3.5 grams of MS powder (Sigma#M0404) are dissolved in 1000 milliliters of water, prepare MS culture solution.It is prepared with MS culture solution
The beet aglucone V inducer of 100mM containing trehalose, methyl jasmonate 5mM.
Two, Momordica grosvenori mogroside V induces:
The 5th day after pollination fruit is handled with Momordica grosvenori mogroside V inducer.Processing mode is using smearing, the 5th after pollination
It is to handling 2 times between 30 days.
Three, it harvests
Fruit maturation harvesting in 70th day after pollination, carries out momordica glycoside V content detection using HPLC method.
Example IV
One, the preparation of Momordica grosvenori mogroside V inducer:
4.8 grams of MS powder (Sigma#M0404) are dissolved in 1000 milliliters of water, prepare MS culture solution.It is prepared with MS culture solution
The beet aglucone V inducer of 700mM containing trehalose, methyl jasmonate 20mM.
Two, Momordica grosvenori mogroside V induces:
The 15th day after pollination fruit is handled with Momordica grosvenori mogroside V inducer.Processing mode is being awarded using smearing or by spraying
It is handled 6 times between the 5th day to 30 days after powder.
Three, it harvests
Fruit maturation harvesting in 90th day after pollination, carries out momordica glycoside V content detection using HPLC method.
Embodiment five
One, the preparation of Momordica grosvenori mogroside V inducer:
3.5 grams of MS powder (Sigma#M0404) are dissolved in 1000 milliliters of water, prepare MS culture solution.It is prepared with MS culture solution
The beet aglucone V inducer of 100mM containing sucrose, methyl jasmonate 5mM.
Two, Momordica grosvenori mogroside V induces:
The 5th day after pollination fruit is handled with Momordica grosvenori mogroside V inducer.Processing mode is using smearing, the 5th after pollination
It is to handling 2 times between 30 days.
Three, it harvests
Fruit maturation harvesting in 70th day after pollination, carries out momordica glycoside V content detection using HPLC method.
Embodiment six
One, the preparation of Momordica grosvenori mogroside V inducer:
4.8 grams of MS powder (Sigma#M0404) are dissolved in 1000 milliliters of water, prepare MS culture solution.It is prepared with MS culture solution
The beet aglucone V inducer of 700mM containing sucrose, methyl jasmonate 20mM.
Two, Momordica grosvenori mogroside V induces:
The 15th day after pollination fruit is handled with Momordica grosvenori mogroside V inducer.Processing mode is being awarded using smearing or by spraying
It is handled 6 times between the 5th day to 30 days after powder.
Three, it harvests
Fruit maturation harvesting in 90th day after pollination, carries out momordica glycoside V content detection using HPLC method.
Embodiment seven
One, the preparation of Momordica grosvenori mogroside V inducer:
4.4 grams of MS powder (Sigma#M0404) are dissolved in 1000 milliliters of water, prepare MS culture solution.It is prepared with MS culture solution
The beet aglucone V inducer of 500mM containing trehalose, methyl jasmonate 10mM.
Two, Momordica grosvenori mogroside V induces:
The 10th day after pollination fruit is handled with Momordica grosvenori mogroside V inducer.Processing mode is using spraying, the after pollination
It is handled 4 times between 5 days to 30 days.
Three, it harvests
Fruit maturation harvesting in 80th day after pollination, carries out momordica glycoside V content detection using HPLC method.
Embodiment eight
One, the preparation of Momordica grosvenori mogroside V inducer:
4.4 grams of MS powder (Sigma#M0404) are dissolved in 1000 milliliters of water, prepare MS culture solution.It is prepared with MS culture solution
The beet aglucone V inducer of 500mM containing sucrose, methyl jasmonate 10mM.
Two, Momordica grosvenori mogroside V induces:
The 10th day after pollination fruit is handled with Momordica grosvenori mogroside V inducer.Processing mode is using spraying, the after pollination
It is handled 4 times between 5 days to 30 days.
Three, it harvests
Fruit maturation harvesting in 80th day after pollination, carries out momordica glycoside V content detection using HPLC method.
Comparative example 1~4 is used to evaluate the synergy of trehalose or sucrose and methyl jasmonate
Comparative example 1
Compared with Example 7, it is added without trehalose, other conditions are identical, specific as follows:
One, the preparation of Momordica grosvenori mogroside V inducer:
4.4 grams of MS powder (Sigma#M0404) are dissolved in 1000 milliliters of water, prepare MS culture solution.It is prepared with MS culture solution
The beet aglucone V inducer of the 10mM containing methyl jasmonate.
Two, Momordica grosvenori mogroside V induces:
The 10th day after pollination fruit is handled with Momordica grosvenori mogroside V inducer.Processing mode is using spraying, the after pollination
It is handled 4 times between 5 days to 30 days.
Three, it harvests
Fruit maturation harvesting in 80th day after pollination, carries out momordica glycoside V content detection using HPLC method.
Comparative example 2
Compared with Example 8, it is added without sucrose, other conditions are identical, specific as follows:
One, the preparation of Momordica grosvenori mogroside V inducer:
4.4 grams of MS powder (Sigma#M0404) are dissolved in 1000 milliliters of water, prepare MS culture solution.It is prepared with MS culture solution
The beet aglucone V inducer of the 10mM containing methyl jasmonate.
Two, Momordica grosvenori mogroside V induces:
The 10th day after pollination fruit is handled with Momordica grosvenori mogroside V inducer.Processing mode is using spraying, the after pollination
It is handled 4 times between 5 days to 30 days.
Three, it harvests
Fruit maturation harvesting in 80th day after pollination, carries out momordica glycoside V content detection using HPLC method.
Comparative example 3
Compared with Example 7, trehalose being replaced using maltose, other conditions are identical, specific as follows:
One, the preparation of Momordica grosvenori mogroside V inducer:
4.4 grams of MS powder (Sigma#M0404) are dissolved in 1000 milliliters of water, prepare MS culture solution.It is prepared with MS culture solution
The beet aglucone V inducer of 500mM containing maltose, methyl jasmonate 10mM.
Two, Momordica grosvenori mogroside V induces:
The 10th day after pollination fruit is handled with Momordica grosvenori mogroside V inducer.Processing mode is using spraying, the after pollination
It is handled 4 times between 5 days to 30 days.
Three, it harvests
Fruit maturation harvesting in 80th day after pollination, carries out momordica glycoside V content detection using HPLC method.
Comparative example 4
Compared with Example 8, lactose replaces sucrose, and other conditions are identical, specific as follows:
One, the preparation of Momordica grosvenori mogroside V inducer:
4.4 grams of MS powder (Sigma#M0404) are dissolved in 1000 milliliters of water, prepare MS culture solution.It is prepared with MS culture solution
The beet aglucone V inducer of 500mM containing lactose, methyl jasmonate 10mM.
Two, Momordica grosvenori mogroside V induces:
The 10th day after pollination fruit is handled with Momordica grosvenori mogroside V inducer.Processing mode is using spraying, the after pollination
It is handled 4 times between 5 days to 30 days.
Three, it harvests
Fruit maturation harvesting in 80th day after pollination, carries out momordica glycoside V content detection using HPLC method.
Comparative example 5~8 is for evaluating Momordica grosvenori mogroside V inducer action time, frequency
Comparative example 5
Compared with Example 7, inducer action time shifts to an earlier date, and other conditions are identical, specific as follows:
One, the preparation of Momordica grosvenori mogroside V inducer:
4.4 grams of MS powder (Sigma#M0404) are dissolved in 1000 milliliters of water, prepare MS culture solution.It is prepared with MS culture solution
The beet aglucone V inducer of 500mM containing trehalose, methyl jasmonate 10mM.
Two, Momordica grosvenori mogroside V induces:
It is handled with Momordica grosvenori mogroside V inducer within the 3rd day after pollination, processing mode uses by spraying, the 5th day to 30 after pollination
It is handled 4 times between it.
Three, it harvests
Fruit maturation harvesting in 80th day after pollination, carries out momordica glycoside V content detection using HPLC method.
Comparative example 6
Compared with Example 7, inducer action time shifts to an earlier date, and other conditions are identical, specific as follows:
One, the preparation of Momordica grosvenori mogroside V inducer:
4.4 grams of MS powder (Sigma#M0404) are dissolved in 1000 milliliters of water, prepare MS culture solution.It is prepared with MS culture solution
The beet aglucone V inducer of 500mM containing trehalose, methyl jasmonate 10mM.
Two, Momordica grosvenori mogroside V induces:
It is handled with Momordica grosvenori mogroside V inducer within the 1st day after pollination, processing mode uses by spraying, the 5th day to 30 after pollination
It is handled 4 times between it.
Three, it harvests
Fruit maturation harvesting in 80th day after pollination, carries out momordica glycoside V content detection using HPLC method.
Comparative example 7
Compared with Example 7, the processing frequency between the 10th day to 30 days is improved, other conditions are identical, specific as follows:
One, the preparation of Momordica grosvenori mogroside V inducer:
4.4 grams of MS powder (Sigma#M0404) are dissolved in 1000 milliliters of water, prepare MS culture solution.It is prepared with MS culture solution
The beet aglucone V inducer of 500mM containing trehalose, methyl jasmonate 10mM.
Two, Momordica grosvenori mogroside V induces:
The 10th day after pollination fruit is handled with Momordica grosvenori mogroside V inducer.Processing mode is using spraying, the after pollination
It is handled 10 times between 5 days to 30 days.
Three, it harvests
Fruit maturation harvesting in 80th day after pollination, carries out momordica glycoside V content detection using HPLC method.
Comparative example 8
Compared with Example 7, the processing frequency between the 10th day to 30 days is improved, other conditions are identical, specific as follows:
One, the preparation of Momordica grosvenori mogroside V inducer:
4.4 grams of MS powder (Sigma#M0404) are dissolved in 1000 milliliters of water, prepare MS culture solution.It is prepared with MS culture solution
The beet aglucone V inducer of 500mM containing trehalose, methyl jasmonate 10mM.
Two, Momordica grosvenori mogroside V induces:
The 10th day after pollination fruit is handled with Momordica grosvenori mogroside V inducer.Processing mode is using spraying, the after pollination
It is handled 15 times between 5 days to 30 days.
Three, it harvests
Fruit maturation harvesting in 80th day after pollination, carries out momordica glycoside V content detection using HPLC method.
As a result with analysis
By the Siraitia grosvenorii after the harvesting in Examples 1 to 8 and comparative example 1~8, Momordica grosvenori mogroside V is carried out using HPLC method
Content detection the results are shown in Table 1, and beet aglucone V content refers to that beet aglucone V content accounts for the percentage of fruit content dry weight in table 1.
The testing result of 1~8 Momordica grosvenori mogroside V content of 1 Examples 1 to 8 of table and comparative example
Compared with comparative example 1, trehalose (concentration 500mM) is added in embodiment 7, and sweet tea salidroside content compares respectively after handling fruit
Control (comparative example 1) improves nearly 40%.Compared with comparative example 2, sucrose (concentration 500mM) is added in embodiment 8, handles fruit
Sweet tea salidroside content improves 46% than control (comparative example 2) respectively after son.Comparative example 3 using maltose replaces trehalose, and (concentration is
500mM), compared with Example 7, sweet tea salidroside content significant difference after processing fruit.Comparative example 4 replaces sucrose using lactose, with reality
It applies example 8 to compare, sweet tea salidroside content differs by about one time after handling fruit.Result, it is believed that trehalose of the present invention or sucrose and jasmonic first
The synergy of ester can promote other low sugar to induce the high expression of momordica glycoside V metabolism route of synthesis key gene
The conversion of base sweet tea glucoside (such as sweet tea glucoside II, sweet tea glucoside IV) from accumulation glucoside V of sweet tea glycosides V to high-glycosylation Siraitia grosvenorii, thus in a short time
Quickly improve the content of mogroside glucoside V.
Comparative example 5~6 shifts to an earlier date inducer action time, but the effect of its induction disappears, comparative example 7~8, subsequent
30 days in processing frequency raising, do not further increase sweet Momordica grosvenori salidroside content, therefore, the present invention in trehalose, jasmine
Sour methyl esters or sucrose, methyl jasmonate action time, frequency equally also be can not ignore.
Although above having used general explanation, specific embodiment and test, the present invention is made to retouch in detail
It states, but on the basis of the present invention, it can be made some modifications or improvements, this is apparent to those skilled in the art
's.Therefore, these modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to claimed
Range.
Claims (8)
1. a kind of method for improving Momordica grosvenori mogroside V content, which is characterized in that the method is before picking using at inducer
Siraitia grosvenorii children okra fruit reality is managed, the inducer is trehalose, methyl jasmonate or sucrose, methyl jasmonate.
2. a kind of method for improving Momordica grosvenori mogroside V content as described in claim 1, which is characterized in that the inducer
Concentration is 100~700mM of trehalose, 5~20mM of methyl jasmonate.
3. a kind of method for improving Momordica grosvenori mogroside V content as described in claim 1, which is characterized in that the inducer
Concentration is 100~700mM of sucrose, 5~20mM of methyl jasmonate.
4. a kind of method for improving Momordica grosvenori mogroside V content as claimed in claim 2 or claim 3, which is characterized in that the inducer
Preparation method it is as follows: 3.5~4.8 grams of MS powder are dissolved in 1000 milliliters of water, prepare MS culture solution, are contained with the preparation of MS culture solution
The beet aglucone V inducer of trehalose, methyl jasmonate or sucrose, methyl jasmonate.
5. a kind of method for improving Momordica grosvenori mogroside V content as described in claim 1, which is characterized in that at the inducer
The mode of reason is for smearing or by spraying.
6. a kind of method for improving Momordica grosvenori mogroside V content as described in claim 1, which is characterized in that at the inducer
The time of reason is to start to process within the 5th~15 day after pollinating.
7. a kind of method for improving Momordica grosvenori mogroside V content as described in claim 1, which is characterized in that at the inducer
The time of reason is the 5th day to the 30th day after pollination, and the total degree of the processing is 2~6 times.
8. a kind of method for improving Momordica grosvenori mogroside V content as described in claim 1, which is characterized in that the picking when
Between for pollination after the 70th~90 day.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910776404.2A CN110447414B (en) | 2019-08-22 | 2019-08-22 | Method for increasing content of mogroside V in momordica grosvenori |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910776404.2A CN110447414B (en) | 2019-08-22 | 2019-08-22 | Method for increasing content of mogroside V in momordica grosvenori |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110447414A true CN110447414A (en) | 2019-11-15 |
| CN110447414B CN110447414B (en) | 2022-04-05 |
Family
ID=68488361
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910776404.2A Active CN110447414B (en) | 2019-08-22 | 2019-08-22 | Method for increasing content of mogroside V in momordica grosvenori |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110447414B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111656996A (en) * | 2020-07-02 | 2020-09-15 | 凯里学院 | Method for preventing and controlling golden autumn pear ring rot by using methyl jasmonate |
| CN111656995A (en) * | 2020-07-02 | 2020-09-15 | 凯里学院 | Method for improving storage quality of autumn pears by using methyl jasmonate |
| CN114292307A (en) * | 2022-01-10 | 2022-04-08 | 江西海富生物工程有限公司 | Method for preparing bitter-taste-free mogroside from naturally immature momordica grosvenori |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100083407A1 (en) * | 2004-09-30 | 2010-04-01 | Ceres, Inc. | Nucleotide sequences and polypeptides encoded thereby useful for modifying plant characteristics |
| CN106797974A (en) * | 2016-12-29 | 2017-06-06 | 广西壮族自治区药用植物园 | The method for improving Simon glycosides I contents in Momordica grosvenori |
| CN107258541A (en) * | 2017-07-28 | 2017-10-20 | 韦荣昌 | Promote the method for Momordica grosvenori UGT7 gene expressions |
| CN107278658A (en) * | 2017-07-28 | 2017-10-24 | 聂天军 | Promote the method for Momordica grosvenori UGT1 gene expressions |
| CN107318654A (en) * | 2017-07-28 | 2017-11-07 | 韦荣昌 | Promote the method for Momordica grosvenori CS gene expressions |
| CN107318651A (en) * | 2017-07-28 | 2017-11-07 | 李华政 | Promote the method for Momordica grosvenori UGT2 gene expressions |
| CN107318652A (en) * | 2017-07-28 | 2017-11-07 | 韦荣昌 | Promote the method for Momordica grosvenori HMGR gene expressions |
| CN107360877A (en) * | 2017-07-28 | 2017-11-21 | 黄小华 | Promote the method for Momordica grosvenori CYP26 gene expressions |
| CN108277197A (en) * | 2017-01-24 | 2018-07-13 | 桂林莱茵生物科技股份有限公司 | A method of improving mogroside Ⅴ content in Siraitia grosvenorii suspension cell |
| CN110024787A (en) * | 2019-04-24 | 2019-07-19 | 南京林业大学 | The methods and applications of plant leaf blade flavonoids and the accumulation of terpene lactones compound are induced using methyl jasmonate |
-
2019
- 2019-08-22 CN CN201910776404.2A patent/CN110447414B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100083407A1 (en) * | 2004-09-30 | 2010-04-01 | Ceres, Inc. | Nucleotide sequences and polypeptides encoded thereby useful for modifying plant characteristics |
| CN106797974A (en) * | 2016-12-29 | 2017-06-06 | 广西壮族自治区药用植物园 | The method for improving Simon glycosides I contents in Momordica grosvenori |
| CN108277197A (en) * | 2017-01-24 | 2018-07-13 | 桂林莱茵生物科技股份有限公司 | A method of improving mogroside Ⅴ content in Siraitia grosvenorii suspension cell |
| CN107258541A (en) * | 2017-07-28 | 2017-10-20 | 韦荣昌 | Promote the method for Momordica grosvenori UGT7 gene expressions |
| CN107278658A (en) * | 2017-07-28 | 2017-10-24 | 聂天军 | Promote the method for Momordica grosvenori UGT1 gene expressions |
| CN107318654A (en) * | 2017-07-28 | 2017-11-07 | 韦荣昌 | Promote the method for Momordica grosvenori CS gene expressions |
| CN107318651A (en) * | 2017-07-28 | 2017-11-07 | 李华政 | Promote the method for Momordica grosvenori UGT2 gene expressions |
| CN107318652A (en) * | 2017-07-28 | 2017-11-07 | 韦荣昌 | Promote the method for Momordica grosvenori HMGR gene expressions |
| CN107360877A (en) * | 2017-07-28 | 2017-11-21 | 黄小华 | Promote the method for Momordica grosvenori CYP26 gene expressions |
| CN110024787A (en) * | 2019-04-24 | 2019-07-19 | 南京林业大学 | The methods and applications of plant leaf blade flavonoids and the accumulation of terpene lactones compound are induced using methyl jasmonate |
Non-Patent Citations (4)
| Title |
|---|
| KAILUN ZHANG等: "Methyl jasmonate-induced accumulation of metabolites and transcriptional responses involved in triterpene biosynthesis in Siraitia grosvenorii fruit at different growing stages", 《ACTA SOCIETATIS BOTANICORUM POLONIAE》 * |
| 李锋等: "《罗汉果栽培与开发利用》", 30 April 2004, 中国林业出版社 * |
| 韦荣昌等: "茉莉酸甲酯对罗汉果SQS、CS和CAS基因表达的影响", 《茉莉酸甲酯对罗汉果SQS、CS和CAS基因表达的影响》 * |
| 韩长日等: "《实用化学品配方手册4》", 31 August 1999, 四川科学技术出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111656996A (en) * | 2020-07-02 | 2020-09-15 | 凯里学院 | Method for preventing and controlling golden autumn pear ring rot by using methyl jasmonate |
| CN111656995A (en) * | 2020-07-02 | 2020-09-15 | 凯里学院 | Method for improving storage quality of autumn pears by using methyl jasmonate |
| CN114292307A (en) * | 2022-01-10 | 2022-04-08 | 江西海富生物工程有限公司 | Method for preparing bitter-taste-free mogroside from naturally immature momordica grosvenori |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110447414B (en) | 2022-04-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110447414A (en) | A method of improving Momordica grosvenori mogroside V content | |
| CN101624560B (en) | Preparation method of kelp fruit wine | |
| CN101294124B (en) | Technique for preparing tartary buckwheat sweet fermented glutinous rice | |
| EP1899455A1 (en) | The method for production of corosolic acid in suspension culture of plant cells | |
| CN101880615A (en) | Preparation method of Lycium ruthenicum fruit wine | |
| CN106719874A (en) | The method for improving momordica grosvenori alcohol content in Momordica grosvenori | |
| CN105211880A (en) | A kind of preparation method of fig ferment | |
| CN104560608B (en) | Red-color dragon fruit vinegar processing process | |
| CN102618430A (en) | Method for preparing pomegranate fruit vinegar | |
| CN108095087A (en) | Solid state fermentation matrimony vine and preparation method thereof | |
| CN110604299B (en) | Rosa roxburghii mushroom oral liquid and preparation method thereof | |
| CN108384682A (en) | Blank corn fruit ear fermented wine rich in anthocyanidin | |
| CN105238642B (en) | A kind of brewing method of low alcohol jerusalem artichoke health liquor | |
| CN103829194B (en) | A kind of preparation method being rich in oligo-glucomannan fermentation lotus root bamboo shoot | |
| KR101307202B1 (en) | Cultured root of mountain ginseng crmg 5 with improved content of ppt type saponin and method of producing the same | |
| CN113142351B (en) | Health vine tea and its production process | |
| CN115400061A (en) | Ginseng callus extract and preparation method and application thereof | |
| CN110283685A (en) | A kind of preparation method of pomegranate wine | |
| KR20160075114A (en) | Preparation Metod of Vinegar Containing Coffee Extract | |
| CN113647327B (en) | Method for converting ginsenoside in adventitious roots of ginseng | |
| CN111925879B (en) | Brewing method of tea beer retaining characteristic substances of Liuan melon slices | |
| KR20230129136A (en) | Composition for antibacterial, antioxidant, or anti-inflammatory comprising fermented extracts of Canavalia gladiata leaf as an active ingredient | |
| KR20160103358A (en) | Wine or vinegar of Orostachys japonicus made from fermented sugar extract of Orostachys japonicus | |
| CN106544224A (en) | A kind of preparation method of feature ice pomegranate wine | |
| CN112956415A (en) | Liquid culture medium for culturing ginseng adventitious roots and culture method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |