CN110440969A - A kind of Patinopecten yessoensis vigor fast appraisement method - Google Patents
A kind of Patinopecten yessoensis vigor fast appraisement method Download PDFInfo
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- CN110440969A CN110440969A CN201910729669.7A CN201910729669A CN110440969A CN 110440969 A CN110440969 A CN 110440969A CN 201910729669 A CN201910729669 A CN 201910729669A CN 110440969 A CN110440969 A CN 110440969A
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- Prior art keywords
- scallop
- closing force
- vigor
- patinopecten yessoensis
- dynamometer
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01L—MEASURING FORCE, STRESS, TORQUE, WORK, MECHANICAL POWER, MECHANICAL EFFICIENCY, OR FLUID PRESSURE
- G01L5/00—Apparatus for, or methods of, measuring force, work, mechanical power, or torque, specially adapted for specific purposes
- G01L5/0028—Force sensors associated with force applying means
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- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06F—ELECTRIC DIGITAL DATA PROCESSING
- G06F17/00—Digital computing or data processing equipment or methods, specially adapted for specific functions
- G06F17/10—Complex mathematical operations
- G06F17/18—Complex mathematical operations for evaluating statistical data, e.g. average values, frequency distributions, probability functions, regression analysis
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The present invention discloses a kind of Patinopecten yessoensis vigor fast appraisement method, it is characterised in that: the method successively carries out according to the following steps: firstly, the left housing of product Patinopecten yessoensis living is fixed on rectangular stone with rapid-curing cutback cement, right shell keeps free movement state；Then, the lower end of dynamometer is hooked at the right shell edge that scallop can be move freely；With needle stimulus scallop outer embrane, stimulus intervals are 30s/ times, costimulation 12 times, the real-time record of data are carried out by the external computer being connected with dynamometer, detects the instantaneous maximum value of the frequency and closing force that are closed in 6 minutes.Using horizontal axis as detection time, the longitudinal axis is that closing force draws closing force waveform diagram, is analyzed using origin software scallop closing force waveform diagram, calculates the average value of moment maximum close power peak value, finally with the vigor of value size evaluation scallop.
Description
Technical field
The present invention relates to the living body field of circulation of aquatic products, especially a kind of Patinopecten yessoensis vigor fast appraisement method.
Background technique
Patinopecten yessoensis is one of very important breed variety in China, has higher economic value and nutritive value.At me
State's product living are most popular product forms.
Product quality evaluation living is both quotient between the premise means and supply chain links for studying scallop quality comparison mechanism
The judgment basis of industry running.In actual production, specification, cissing rate and coefficient of condition are the indexs that industry tentatively uses at present；In
In field of scientific study, then the fresh-keeping theory and technology of fish is continued to use mostly.Usually used index have ATP association compound,
Muscle pH value, glycogen content, free amino acid etc..Wherein ATP and phosphoric acid proper energy preferably react product quality living, but due to
It extracts and needs to be not easy to catch, transport and consume in shellfish using large-scale instruments such as efficient liquid phases in complicated and continuous mode
Scene be measured.And continuous mode needs to consume several hours, the real-time monitoring being unable to satisfy in the entire process of circulation
Demand, therefore a kind of method or apparatus for being able to solve the above problem is needed now.
Summary of the invention
The present invention is proposed a kind of existing in fishing, transport and consumption to solve above-mentioned deficiency present in the prior art
Field at any time can convenient and quick measurement Patinopecten yessoensis Bush Vitality's method.
The technical solution of the invention is as follows: a kind of Patinopecten yessoensis vigor fast appraisement method, it is characterised in that: described
Method successively carries out according to the following steps:
Firstly, the left housing of product Patinopecten yessoensis living is fixed on rectangular stone with rapid-curing cutback cement, right shell keeps free movement state,
Placing 30 minutes under room temperature environment makes cement solidification, to fix scallop,
Then, the lower end of dynamometer is hooked at the right shell edge that scallop can be move freely, with needle stimulus scallop outer embrane, between stimulation
It is divided into 30s/ times, costimulation 12 times, the real-time record of data is carried out by the external computer being connected with dynamometer, is detected at 6 points
The instantaneous maximum value of the frequency and closing force that are closed in clock, using horizontal axis as detection time, the longitudinal axis is that closing force draws closure Reeb
Shape figure,
Closing force waveform diagram is analyzed, scallop is closed using origin software and generates frequency and the progress of moment peak-peak
Statistics, calculates the average value of its moment closing force, to evaluate the vigor of scallop, calculation formula are as follows: and closing force average value (N)=
Each peak value summation/closure frequency.
The present invention compared with the existing technology, has the advantages that
Patinopecten yessoensis vigor fast appraisement method of the present invention utilizes detection scallop ATP and phosphoric acid proper energy for traditional
Mode evaluate the problems of the mode of scallop vigor, design a kind of simple evaluation method, this method is by closing
Resultant force size reacts the contraction power of scallop muscle, obtains the variation waveform diagram of closing force.Then waveform diagram will be obtained to carry out
Parsing, calculates Fast Evaluation index of the average value as scallop vigor of closing force；This method and classical biochemical assessment method
(traditional method using large scale equipment detection scallop ATP and phosphoric acid proper energy) result is positively correlated, and method is simple and easy, most heavy
What is wanted is that can carry out Real-Time Evaluation, it can be said that it has a variety of advantages, particularly suitable in the shellfish product field of circulation living
Middle popularization and application, market prospects are very wide.
Detailed description of the invention
Fig. 1 is the schematic diagram of the used equipment of the method for the invention.
Fig. 2 is the variation waveform diagram of scallop closing force.
Fig. 3 is scallop ATP content and closing force variation scatter plot.
Specific embodiment
Below in conjunction with Detailed description of the invention a specific embodiment of the invention, as shown in Figure 1, Figure 2, Figure 3 shows: a kind of Patinopecten yessoensis
Vigor fast appraisement method successively carries out according to the following steps:
Firstly, the left housing of product Patinopecten yessoensis living is fixed on rectangular stone with rapid-curing cutback cement, right shell keeps free movement state,
Placing 30 minutes under room temperature environment makes cement solidification, to fix scallop,
Then, when the antenna in scallop outer embrane is sufficiently spread out, keeps naturally open state, the lower end of dynamometer is hooked in scallop
The right shell edge that can be move freely,
With needle stimulus scallop outer embrane, stimulus intervals are 30s/ times, costimulation 12 times, pass through the external computer being connected with dynamometer
The real-time record of data is carried out, the instantaneous maximum value of the frequency and closing force that are closed in 6 minutes is detected, is detection with horizontal axis
Time, the longitudinal axis are that closing force draws closing force waveform diagram,
Closing force waveform diagram is analyzed, scallop is closed using origin software and generates frequency and the progress of moment peak-peak
Statistics, calculates the average value of its moment closing force, to evaluate the vigor of scallop, calculation formula are as follows: and closing force average value (N)=
Each peak value summation/closure frequency.
Specific embodiment:
1. selected commercial specification scallop: the high about 10.5cm of shell, shell are about 10cm, shell thickness about 2.0cm, weight about 120g, and health is complete
Good living body 60.
2. the fixation of Patinopecten yessoensis: the left housing of Patinopecten yessoensis being fixed on rectangular stone with rapid-curing cutback cement, keeps right shell
It can move freely.
3. Patinopecten yessoensis vigor standardizes: fixed scallop being put into the seawater slot of flowing and is temporarily supported 24 hours, scallop is made
It is thus capable of sufficiently recovering vigor state.
4. applying dry dew stress: it is restored to the scallop of optimum state, is taken out from seawater, is divided into 6 groups, every group 10,
It chooses 5 in each group to be handled with this method, another 5 then (utilize conventional method with the ATP content that conventional method measures its muscle
Measuring muscle ATP content is reference group).This 6 groups of scallops place 0h, 6h, 8h, 12h, 36h, 48h in 4 DEG C of refrigerators respectively.
5. scallop closing force measures: according to shown in Fig. 1, respectively measurement apply stimulation (30s/ times) afterwards Patinopecten yessoensis 6
Closure frequency and moment maximum close power in min.The frequency of closure is between 10-20 times, and closing force is between 1-25 N.
Each scallop carries out closing force acquisition according to aforesaid operations.Calculating every group of scallop, closing force is instantaneously most in 6 minutes
The average value (AVERAGE (N1:N5)) being worth greatly, the index as each group scallop vigor.
6. ATP is associated with compound assay in scallop adductor muscle: being intercepted on the scallop for passing through said determination from each
1.0 g shellfish meats and with 1:10 be added 5% perchloric acid (PCA), 2 mol/L KOH tune pH are added after sufficiently smashing to pieces in ice bath extremely
2.0~3.5, add ultrapure water to be settled to 20 mL, 5 min are centrifuged under the conditions of 3000 g, takes supernatant with 0.45 μm of filter membrane mistake
Filter.It takes 4 mL filtrates and 1 mL, 0.1 mol/L phosphate buffer (pH 7.5) is added.Whole process is under condition of ice bath
It carries out.
It is analyzed using high performance liquid chromatography.Chromatographic column: Dalian is according to Lyntech Corporation (US) 10177 South 77th East Avenue Tulsa, Oklahoma 74133 U.S. (5 μ of SinoChrom ODS-BP
M, 4.6 mm × 250 mm)；Detector: diode array detector (DAD)；Detection wavelength: 254 nm；Temperature: 35 DEG C；Stream
Speed: 0.7 mL/min；Sample volume: 0.02 mL.Mobile phase A: 0.05 mol/L K2HPO4-KH2PO4 buffer (pH 6.5),
Mobile phase B: mobile phase A: methanol solution=(8: 2).
Data analysis: using temperature of exposure and dry as abscissa, closing force and ATP content are analyzed respectively as ordinate.Knot
Fruit shows: with the extension of temperature of exposure and dry, the closing force of scallop gradually decays to 6 N from 14 N.ATP content is from 5.6 μm of ol/g
Drop to 3.6 μm of ol/g.Downward trend is presented in closing force size and ATP content.To the correlation analysis of two indices (see
Table 1), the related coefficient of closing force size and ATP content is 0.94, illustrates variation of the closing force with ATP content in extremely significant phase
Closing property (P < 0.01).
Claims (1)
1. a kind of Patinopecten yessoensis vigor fast appraisement method, it is characterised in that: the method successively carries out according to the following steps:
Firstly, the left housing of product Patinopecten yessoensis living is fixed on rectangular stone with rapid-curing cutback cement, right shell keeps free movement state,
Placing 30 minutes under room temperature environment makes cement solidification, to fix scallop,
Then, the lower end of dynamometer is hooked at the right shell edge that scallop can be move freely,
With needle stimulus scallop outer embrane, stimulus intervals are 30s/ times, costimulation 12 times, pass through the external computer being connected with dynamometer
The real-time record of data is carried out, the instantaneous maximum value of the frequency and closing force that are closed in 6 minutes is detected, is detection with horizontal axis
Time, the longitudinal axis are that closing force draws closing force waveform diagram,
Closing force waveform diagram is analyzed, scallop is closed using origin software and generates frequency and the progress of moment peak-peak
Statistics, calculates the average value of its moment closing force, to evaluate the vigor of scallop, calculation formula are as follows: and closing force average value (N)=
Each peak value summation/closure frequency.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113933471A (en) * | 2021-10-16 | 2022-01-14 | 中国海洋大学 | Method for evaluating quality and character of scallop based on tension index |
| CN113951884A (en) * | 2021-10-16 | 2022-01-21 | 中国海洋大学 | Method for measuring tension of adductor muscle of bivalve shellfish |
| CN114158502A (en) * | 2021-12-09 | 2022-03-11 | 中国海洋大学 | Method for rapidly evaluating resistance of bivalve based on tension index |
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| CN113933471A (en) * | 2021-10-16 | 2022-01-14 | 中国海洋大学 | Method for evaluating quality and character of scallop based on tension index |
| CN113951884A (en) * | 2021-10-16 | 2022-01-21 | 中国海洋大学 | Method for measuring tension of adductor muscle of bivalve shellfish |
| CN113933471B (en) * | 2021-10-16 | 2022-06-21 | 中国海洋大学 | Method for evaluating quality character of scallop based on tension index |
| CN114158502A (en) * | 2021-12-09 | 2022-03-11 | 中国海洋大学 | Method for rapidly evaluating resistance of bivalve based on tension index |
| CN114158502B (en) * | 2021-12-09 | 2022-08-23 | 中国海洋大学 | Method for rapidly evaluating resistance of bivalve based on tension index |
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