CN101810655A - Preparation technology and antioxidation activity of Panax sessiliflorus leaf extract - Google Patents
Preparation technology and antioxidation activity of Panax sessiliflorus leaf extract Download PDFInfo
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- CN101810655A CN101810655A CN 201010163028 CN201010163028A CN101810655A CN 101810655 A CN101810655 A CN 101810655A CN 201010163028 CN201010163028 CN 201010163028 CN 201010163028 A CN201010163028 A CN 201010163028A CN 101810655 A CN101810655 A CN 101810655A
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- panax sessiliflorus
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Abstract
The invention relates to preparation technology and antioxidation activity of Panax sessiliflorus leaf extract, belonging to the technical field of research and development of medical products. The technology is that Panax sessiliflorus leaves are added in 70 percent ethanol with weight 6-8 times of the weight of the Panax sessiliflorus leaves and are extracted for 8-10h at room temperature, gauze is used for filtration, filter residue is repetitively undergone the steps for one time by using 70 percent ethanol with weight respectively six times and four times of the weight of the Panax sessiliflorus leaves, filter liquid obtained at three times are merged, the ethanol is recovered, the Panax sessiliflorus leaf extract is obtained, the Panax sessiliflorus leaf extract is added with a proper amount of distilled water, the obtained mixture is sealed and treated by ultrasonic waves for 30min, temperature is 30 DEG C, power is 80W and an antioxidation test is conducted after the Panax sessiliflorus leaf extract is fully dissolved. The product of the invention has antioxidation activity.
Description
Technical field
Medical product research and development technical field under the present invention.The present invention relates to the preparation technology and the antioxidant activity of Panax sessiliflorus leaf extract.Product of the present invention has antioxidant activity.
Background technology
The present invention relates to the preparation technology and the antioxidant activity of Panax sessiliflorus leaf extract.Acanthopanax sessiliflorus(Rupr.et Maxim.) Seem. Acanthopanaxsessiliflorus (Rupr.et Maxim.) Seem. is the Araliaceae acanthopanax plant, is distributed in ground such as northeast, North China, Shaanxi, in mountain region, two sides, streams, hilly upland, border and bushes growth is arranged also.Warm in nature, acrid in the mouth, nontoxic, pharmacological research shows that Panax sessiliflorus leaf has effects such as strengthening by means of tonics, expelling wind and removing dampness.People use Acanthopanax sessiliflorus(Rupr.et Maxim.) Seem. preparation for treating leukopenia, vertigo and menopausal syndrome now.Along with further development and utilization to Acanthopanax sessiliflorus(Rupr.et Maxim.) Seem., developed the Acanthopanax sessiliflorus(Rupr.et Maxim.) Seem. Folium Ligustri pubescentis on the market, have the effect of fat-reducing, blood sugar lowering, tranquillizing and allaying excitement.Yet the antioxidation to Panax sessiliflorus leaf rarely has report.
Human body can constantly produce various free radicals in the oxidative metabolism process of vital movement, too much radical pair biomacromolecule causes damage in the body, cause the 26S Proteasome Structure and Function of cell and tissue to go down, and the aging course of body is exerted an influence, finally cause the generation of disease.And the antioxidant of synthetic has certain toxicity and carcinogenesis.So estimate and screen and have the new trend that the active natural plants of strong anti-oxidation has become medical science, biology and food scientific research.
Adopting typical antioxidation in vitro system, is contrast with the ascorbic acid, estimates the antioxidant activity of Panax sessiliflorus leaf extract, provides theoretical foundation for better developing the edible resource of Acanthopanax sessiliflorus(Rupr.et Maxim.) Seem..
Summary of the invention
1. the present invention relates to the preparation technology and the antioxidant activity of Panax sessiliflorus leaf extract, it is characterized in that the technology for preparing Panax sessiliflorus leaf extract comprises following process:
Step (one): Panax sessiliflorus leaf is added 8-10 doubly measure lixiviate 8-10h under the 70% ethanol room temperature, use filtered through gauze then, filtering residue repeats above step respectively once with the ethanol of 6 times of amounts and 4 times of amounts 70% respectively again, obtains filtrate;
Step (two): the filtrate in the step () is merged, reclaim ethanol, get Panax sessiliflorus leaf extract;
Step (three): the Panax sessiliflorus leaf extract in the step (two) is added an amount of distilled water, seal ultrasonic 30min, temperature is 30 ℃, and power is 80W, treat its dissolving fully after, carry out the antioxidation test, Panax sessiliflorus leaf extract has antioxidation.
2. the raw material according to preparation Panax sessiliflorus leaf extract of the present invention is the leaf of Acanthopanax sessiliflorus(Rupr.et Maxim.) Seem. Acanthopanaxsessiliflorus (Rupr.et Maxim.) Seem..
According to the present invention, " % " among the present invention is percentage by weight.
The specific embodiment
Preparation technology and the antioxidant activity that relates to Panax sessiliflorus leaf extract of the present invention comprises following examples, and the following examples can further specify the present invention, but do not limit the present invention in any way.
Embodiment 1:
Step (one): Panax sessiliflorus leaf is added lixiviate 8h under 10 times of amount 70% ethanol room temperatures, use filtered through gauze then, filtering residue repeats above step respectively once with the ethanol of 6 times of amounts and 4 times of amounts 70% respectively again, obtains filtrate;
Step (two): the filtrate in the step () is merged, reclaim ethanol, get Panax sessiliflorus leaf extract;
Step (three): the Panax sessiliflorus leaf extract in the step (two) is added an amount of distilled water, seal ultrasonic 30min, temperature is 30 ℃, and power is 80W, treat its dissolving fully after, carry out the antioxidation test, Panax sessiliflorus leaf extract has antioxidation.
Embodiment 2:
Step (one): Panax sessiliflorus leaf is added lixiviate 10h under 8 times of amount 70% ethanol room temperatures, use filtered through gauze then, filtering residue repeats above step respectively once with the ethanol of 6 times of amounts and 4 times of amounts 70% respectively again, obtains filtrate;
Step (two): the filtrate in the step () is merged, reclaim ethanol, get Panax sessiliflorus leaf extract;
Step (three): the Panax sessiliflorus leaf extract in the step (two) is added an amount of distilled water, seal ultrasonic 30min, temperature is 30 ℃, and power is 80W, treat its dissolving fully after, carry out the antioxidation test, Panax sessiliflorus leaf extract has antioxidation.
The antioxidation test of Panax sessiliflorus leaf extract
1 Panax sessiliflorus leaf extract is to the mensuration of DPPH clearance rate
1.1DPPH the preparation of solution
DPPH sample 0.01g decided in accurate title, adds dehydrated alcohol and be settled in the 250ml volumetric flask, obtains DPPH solution, and concentration is 0.04mg/ml.
1.2 the preparation of sample solution
Precision takes by weighing Panax sessiliflorus leaf extract 0.4g and places 50ml tool plug volumetric flask, adds an amount of distilled water, seals ultrasonic 30min, treat its dissolving fully after, add distilled water again and be settled to 50ml, obtain the sample mother solution, concentration is 8mg/ml, and is standby.In the sample mother solution, pipetting volume with the liquid-transfering gun precision is 0.003125,0.00625,0.0125,0.025,0.05,0.1,0.2, the sample mother solution of 0.4ml, obtaining concentration with the distilled water standardize solution in the 10ml volumetric flask is 0.0025,0.005,0.01,0.02,0.04,0.08,0.16, the sample solution of 0.32mg/ml.
1.3 sample is to the scavenging action of DPPH
The sample solution that accurately pipettes variable concentrations is mixed in the different test tubes with DPPH solution and ethanol solution equal-volume respectively, shakes up, and places 30min in the dark, measures its absorbance and be respectively A under 515nm
iAnd A
jOther gets distilled water and DPPH solution equal-volume is mixed in the test tube, and recording absorbance is A.Wherein be mixed into the blank test with distilled water and dehydrated alcohol.And by following formula calculating clearance rate.
Clearance rate=(A-A
i+ A
j)/A * 100%
1.4 50% (IC
50) calculating of inhibition concentration
Utilize the SPSS16.0 software analysis, draw IC
50Value.
2 Panax sessiliflorus leaf extracts are to the mensuration of hydroxy radical clearance rate
2.1 the preparation of sample solution
Precision takes by weighing Panax sessiliflorus leaf extract 0.25g and places 50ml tool plug volumetric flask, adds an amount of distilled water, seals ultrasonic 30min, treat its dissolving fully after, be settled to scale, obtain the sample mother solution, standby.In the sample mother solution, pipetting volume with the liquid-transfering gun precision is 5 μ l, 10 μ l, 20 μ l, 40 μ l, 80 μ l, 160 μ l, 320 μ l, the sample mother solution of 640 μ l, obtaining concentration with the distilled water standardize solution in the 10ml volumetric flask is 0.0025,0.005,0.01,0.02,0.04,0.08,0.16, the sample solution of 0.32mg/ml.
2.2 the preparation of reagent
Take by weighing 0.2032g crystal violet and use the deionized water standardize solution in the 100ml volumetric flask, standby.Transfer Tris buffer solution PH to be 5 with hydrochloric acid, standby.Get 30% H
2O
2Solution 1.0mL, in the 100mL volumetric flask, standby with the deionized water standardize solution.Take by weighing 0.0695gFeSO
47H
2O water standardize solution in the 50mL volumetric flask, standby (matching while using).
2.3 the generation of hydroxy radical OH and mensuration
Get the crystal violet solution 2mL for preparing, Tris buffer 1mL puts into the graded tube of 10mL in order.And be settled to scale with deionized water, and shake up and leave standstill 30 minutes, be designated as A578.
Get the crystal violet solution 2mL for preparing, Tris buffer 1mL, Fe
2+Solution 1mL, H
2O
2Solution 1mL puts into the graded tube of 10mL in order.And be settled to scale with deionized water, and shake up and leave standstill 30 minutes, be designated as A
0
Get the crystal violet solution 2mL for preparing, Tris buffer 1mL, the liquid 2mL to be measured of variable concentrations gradient, Fe
2+Solution 1mL, H
2O
2Solution 1mL puts into the graded tube of 10mL in order.And be settled to scale with deionized water, shake up and leave standstill 30 minutes.Parallel three times, be designated as As.
Do blank experiment with deionized water, under 578 wavelength, measure its absorbance with 721 ultraviolet spectrophotometers.
2.4 the calculating of hydroxy radical OH clearance rate
Utilize formula: clearance rate %=(As-A
0)/(A
578-A
0) * 100% draws the clearance rate of sample extracting solution to hydroxy radical.
2.5 50% inhibition concentration (IC
50) calculating
Utilize the SPSS16.0 software analysis, draw IC
50Value.
3 Panax sessiliflorus leaf extract antioxidation results
3.1 Panax sessiliflorus leaf extract is to the clearance rate of DPPH and hydroxy radical
See Table 1.
Table 1 sample solution is to the clearance rate result of DPPH and hydroxy radical
3.2 vitamin C (VC) is to the clearance rate of DPPH and hydroxy radical
See Table 2.
Table 2VC is to the clearance rate result of DPPH and hydroxy radical
3.3 utilize the data in SPSS16.0 statistical analysis 3.1 and 3.2
See Table 3.
Table 3 Panax sessiliflorus leaf and V
CRemove the EC of DPPH
50The IC of value and OH
50Value
Claims (2)
1. the present invention relates to the preparation technology and the antioxidant activity of Panax sessiliflorus leaf extract, it is characterized in that the technology for preparing Panax sessiliflorus leaf extract comprises following process:
Step (one): Panax sessiliflorus leaf is added 8-10 doubly measure lixiviate 8-10h under the 70% ethanol room temperature, use filtered through gauze then, filtering residue repeats above step respectively once with the ethanol of 6 times of amounts and 4 times of amounts 70% respectively again, obtains filtrate;
Step (two): the filtrate in the step () is merged, reclaim ethanol, get Panax sessiliflorus leaf extract;
Step (three): the Panax sessiliflorus leaf extract in the step (two) is added an amount of distilled water, seals ultrasonic 30min, treat its dissolving fully after, carry out the antioxidation test, Panax sessiliflorus leaf extract has antioxidation.
2. the raw material according to preparation Panax sessiliflorus leaf extract of the present invention is the leaf of Acanthopanax sessiliflorus(Rupr.et Maxim.) Seem. Acanthopanaxsessiliflorus (Rupr.et Maxim.) Seem..
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102512571A (en) * | 2012-01-01 | 2012-06-27 | 山东大学威海分校 | Compound traditional Chinese medicinal preparation and antioxidant activity thereof |
| CN102512506A (en) * | 2012-01-01 | 2012-06-27 | 山东大学威海分校 | Compound acanthopanax root preparation capable of improving activity of superoxide dismutase |
| CN104628878A (en) * | 2015-01-30 | 2015-05-20 | 中华人民共和国北仑出入境检验检疫局 | Crateva unilocalaris Buch. polysaccharide extraction and purification method and application of crateva unilocalaris Buch. polysaccharide |
| CN107048384A (en) * | 2017-03-27 | 2017-08-18 | 西南民族大学 | Acanthopanax giraldii Harms leaf and lily resist oxygen lack purposes |
-
2010
- 2010-04-02 CN CN 201010163028 patent/CN101810655A/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| 《中国农业科学院学位论文》 20090601 王莉飞 无梗五加抗氧化活性成分及质量评价研究 12、13 1-2 , 2 * |
| 《中国药学杂志》 19970131 王广树，等 无梗五加叶化学成分的研究 11-13页 1-2 第32卷, 第1期 2 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102512571A (en) * | 2012-01-01 | 2012-06-27 | 山东大学威海分校 | Compound traditional Chinese medicinal preparation and antioxidant activity thereof |
| CN102512506A (en) * | 2012-01-01 | 2012-06-27 | 山东大学威海分校 | Compound acanthopanax root preparation capable of improving activity of superoxide dismutase |
| CN104628878A (en) * | 2015-01-30 | 2015-05-20 | 中华人民共和国北仑出入境检验检疫局 | Crateva unilocalaris Buch. polysaccharide extraction and purification method and application of crateva unilocalaris Buch. polysaccharide |
| CN104628878B (en) * | 2015-01-30 | 2016-09-14 | 中华人民共和国北仑出入境检验检疫局 | A kind of tree head dish Polyose extraction purification process and application thereof |
| CN107048384A (en) * | 2017-03-27 | 2017-08-18 | 西南民族大学 | Acanthopanax giraldii Harms leaf and lily resist oxygen lack purposes |
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Open date: 20100825 |