CN106924117A - The washing product composition prepared with remaining artemisia annua residue after extraction qinghaosu - Google Patents
The washing product composition prepared with remaining artemisia annua residue after extraction qinghaosu Download PDFInfo
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- CN106924117A CN106924117A CN201710236589.9A CN201710236589A CN106924117A CN 106924117 A CN106924117 A CN 106924117A CN 201710236589 A CN201710236589 A CN 201710236589A CN 106924117 A CN106924117 A CN 106924117A
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- qinghaosu
- artemisia annua
- extracted
- washing product
- flavones
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/10—Washing or bathing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
Abstract
The present invention relates to technical field of plant extraction and daily technology of fine chemicals, and in particular to a kind of washing product composition containing plant extracts.The washing product composition of remaining artemisia annua residue preparation after qinghaosu is extracted in a kind of utilization, is contained：Using the flavones for extracting the preparation of the artemisia annua residue after qinghaosu；The volatile oil that the artemisia annua residue Solvent Extract methods after qinghaosu and flavones are obtained is extracted successively；And washing product auxiliary material.The present invention prepares washing product composition using the artemisia annua residue after qinghaosu has been extracted, and realizes the regeneration to the resource.The washing product composition is used and extracts flavones and volatile oil that remaining artemisia annua residue after qinghaosu extracts as active ingredient, have to user it is significant it is anti-oxidant, remove free radical, whitening, fungistatic effect.
Description
Technical field
The present invention relates to technical field of plant extraction and daily technology of fine chemicals, and in particular to one kind contains plant
The washing product composition of thing extract.
Background technology
Artemisia annua（Artemisia annua Linn）Yellow wormwood artemisia is called, is the annual herb plant of composite family artemisia, extensively
Distribution each province at home, is Chinese tradition Chinese herbal medicine, be have developed on the basis of qinghaosu various derivative dihydroartemisinines,
Artesunate, Artemether, arteether, have antimalarial, resist pregnant, anti-fibrosis, anti-schistosome, resisting toxoplasmosis, anti-arrhythmia and
Cytotoxicity etc. is acted on.Current qinghaosu is used for the value of malaria control by human knowledge and receiving, world health group
Knit the choice drug for the compound preparation of qinghaosu being classified as preventing and treating malaria in the world.According to the statistics of World Health Organization, from
From 2000, about 2.4 hundred million populations of Sub-Saharan Africa area benefit from qinghaosu conjoint therapy, and about 1,500,000 people are because of the therapy
Avoid death caused by malaria.
The usage amount of artemisia annua is huge, extracts the artemisia annua residue after qinghaosu and is largely discarded, these artemisia annua residues
How to be recycled, continue to play value, be the problem for being worth further investigation.Chinese patent application
201210270400.5 disclose a kind of artemisia annua and artemisia annua industrial abstract residue as preparing caffeoyl guinic acid raw material
Application, for the recycling of artemisia annua residue provides an outlet.
The content of the invention
Based on this, the technical problems to be solved by the invention are to provide a kind of utilization and extract remaining artemisia annua after qinghaosu
Washing product composition prepared by residue, the washing product composition is extracted using remaining artemisia annua residue after extracting qinghaosu
Flavones and volatile oil as active ingredient, have to user it is significant it is anti-oxidant, remove free radical, whitening, fungistatic effect.
The present invention solve above-mentioned technical problem technical scheme be：
The washing product composition of remaining artemisia annua residue preparation after qinghaosu is extracted in a kind of utilization, is contained：It is blue or green using extracting
Flavones prepared by the artemisia annua residue after artemisin；The artemisia annua residue Solvent Extract methods after qinghaosu and flavones are extracted successively
The volatile oil for obtaining；And washing product auxiliary material.
Preferably, the flavones and the mass ratio of volatile oil are 20 ~ 40：60~80.Inventor's research discovery, single Huang
Ketone or volatile oil is not obvious to the elimination effect of hydroxy radical, but flavones and the mixed effect of volatile oil will than single component
It is good, and when blending constituent ratio is in 60 ~ 80 volatile oil：During 20% ~ 40% flavones, its effect is most obvious, and elimination efficiency peaks.
Preferably, the flavones accounts for the 1 ~ 1.5% of washing product composition weight with the gross mass of volatile oil.
Preferably, the extracting method of the flavones is：
（1）The constant temperature in 60 DEG C of baking ovens of the artemisia annua residue after qinghaosu will be extracted to dry to constant weight, crushing, mistake in pulverizer will be put into
Sieve, sealing preserve is standby；
（2）By step（1）Residue obtained powder ethanol solution is aided with ultrasonic wave extraction, artemisia annua residue is obtained after filtering always yellow
Ketone extract solution；
（3）Step（2）Resulting material concentration removal ethanol, centrifugation takes supernatant, obtains flavones crude extract after going the removal of impurity；
（4）Step（3）Gained flavones crude extract is purified using macroporous adsorbent resin column chromatography method, obtains flavones.
It is further preferred that step（2）Residue powder is 1g with the solid-liquid ratio of ethanol solution:10 ~ 40 mL, ethanol solution
Percent by volume be 30% ~ 60%, extraction time be 20 ~ 50min, Extracting temperature be 40 ~ 60 DEG C, ultrasonic power is 300W.
Still more preferably, step（2）Residue powder is 1g with the solid-liquid ratio of ethanol solution:40 mL, ethanol solution
Percent by volume be 40%, extraction time is 20min, and Extracting temperature is 60 DEG C, and ultrasonic power is 300W.Determined through experiment,
Under these conditions, the recovery rate highest of general flavone, can reach 7.85%.
Further, the extracting method of the volatile oil is：Use the chrysanthemum extracted after qinghaosu extracted after general flavone
Wormwood artemisia residue, uses organic solvent reflux extraction, obtains backflow and takes out immersion liquid, and volatile oil is obtained after volatilization concentration, refined purification.
Preferably, the extracting method of the volatile oil is：Use the artemisia annua extracted after qinghaosu extracted after general flavone
Residue, by solid-liquid ratio 1g：The ratio of 10ml adds n-hexane, is put into thermostatic mixer, connects reflux condensing tube, adjusts rotating speed
It is 60r/s, temperature is 75 DEG C, refluxing extraction 3 hours, the immersion liquid collected by suction that flows back repeats to extract artemisia annua residue, backflow is taken out
Volatilization concentration is carried out after immersion liquid mixing, and uses anhydrous alcohol solution, sealing is placed on -20 DEG C of refrigerator overnights, and next day suction filtration is removed
Precipitate is removed, ethanol extract is collected, vacuum distillation removes ethanol, obtains blackish green volatile oil.
The washing product composition is shower cream.Can also be skin cream, mildy wash, face cream, toner, Essence etc.
Product.Washing product composition auxiliary material used is that cosmetics lead routine techniques, therefore is not elaborated.
The present invention has the advantages that：
The present invention prepares washing product composition using the artemisia annua residue after qinghaosu has been extracted, and realizes to the resource
Regeneration.The flavones and volatile oil that the washing product composition is extracted using remaining artemisia annua residue after extraction qinghaosu are made
It is active ingredient, there is significant anti-oxidant, removing free radical, whitening, fungistatic effect to user.
Brief description of the drawings
Fig. 1 is with clearance rate broken line graph of the flavones to hydroxy radical for extracting the remaining artemisia annua extraction of qinghaosu.
Fig. 2 is with the peroxide value curve map for extracting the flavones that the remaining artemisia annua of qinghaosu is extracted.
Fig. 3 is the inhibition curve map with the tyrosinase for extracting the flavones that the remaining artemisia annua of qinghaosu is extracted.
Fig. 4 is the fungistatic effect figure of present invention gained volatile oil.
Fig. 5 is the shower cream prepared to embodiment to the clearance rate curve map of hydroxy radical.
Fig. 6 is the inhibiting rate curve map of the shower cream to tyrosinase of embodiment preparation.
Specific embodiment
The present invention will be described in detail with reference to the accompanying drawings and examples.
Embodiment
The shower cream of remaining artemisia annua residue preparation after qinghaosu is extracted in a kind of utilization, containing using after extracting qinghaosu
Artemisia annua residue prepare flavones, extract the artemisia annua residue after qinghaosu and general flavone successively and obtained with Solvent Extract methods
Volatile oil and washing product auxiliary material.
Wherein, the extracting method of flavones is：
1）The constant temperature in 60 DEG C of baking ovens of the artemisia annua residue after qinghaosu will be extracted to dry to constant weight, crushing in pulverizer will be put into, and
40 mesh sieves are crossed, sealing preserve is standby.
2）The above-mentioned residue powder of accurate weighing, by solid-liquid ratio 1g：40 mL add the ethanol that volume parts are 40%, extract
20min, 60 DEG C of water bath with thermostatic control, 300 W ultrasonic wave addeds are repeated to extract twice, and extract solution mixing twice, filtering obtain artemisia annua
Residue general flavone extract solution.
3）Artemisia annua residue general flavone extract solution is concentrated in 60 DEG C of constant temperature blender with magnetic force, 150r/min, until complete
Untill full removing ethanol（With reference to the volume fraction and volume that add ethanol, or until can't smell alcohol smell）, under 3000r/min
Centrifugation 10min, takes supernatant in 50mL volumetric flasks, and precipitation plus the in right amount dissolving of distillation water washing are centrifuged again, and supernatant is Huang
Ketone crude extract.Determined through NaNO2-Al (NO3) 3-NaOH methods, the extraction rate reached of flavones crude extract to 7.85%.
4）Step 3）Gained flavones crude extract is purified using macroporous adsorbent resin column chromatography method, obtains flavones.
Antioxygenic property to resulting flavones purified is determined as follows：
Removing to hydroxy radical
The method reacted with reference to Fenton sets up reaction system（Fe2++H2O2→Fe+OH-+·OH）Model, produces OH, but
Because OH has reactivity very high, the time-to-live is short, if adding salicylic acid in reaction system, just can effectively catch
OH is caught, and produces color products, the product has strong absorption at 510nm, if add to have in this reaction system removing
The measured object of OH functions, will compete OH with salicylic acid, so that color products growing amount is reduced, using fixation response time
Method, adds a series of prepare liquid of concentration gradients, with prepare liquid blank group, surveyed at 510nm in the reaction system of same volume
The different light absorption value of amount.
Some 10mL centrifuge tubes are taken, 0.3mL 9mmol/L FeSO4 solution, 0.3mL 9mmol/L bigcatkin willows is sequentially added
Acid-ethanol solution, the sample liquid of various concentrations is mixed, and adds 0.3mL8.8mmol/L H2O2 solution.Centrifuge tube is placed in 37
30min, flowing water cooling are reacted in DEG C constant water bath box, each pipe is separately added into steaming feedback water, makes system final volume for 5mL,
3000r/min is centrifuged 10min, takes supernatant and light absorption value A0, Ax and Ax0 are determined at lower 510nm, is repeated 3 times measure.Its
Clearance rate to light base free radical is calculated as follows：
Clearance rate=[1-（AX-AX0)/A0]×100%
In clearance rate formula:A0 is blank sample liquid light absorption value（Not sample adding liquid）；Ax is analyte sample fluid light absorption value；Ax0 is this
Bottom light absorption value（Sample adding liquid）.
Experimental record table
Clearance rate=[1-（AX-AX0)/A0]×100%
In clearance rate formula:A0 is blank sample liquid light absorption value（Not sample adding liquid）；Ax is analyte sample fluid light absorption value；Ax0 is this
Bottom light absorption value（Sample adding liquid）.
From the broken line graph of Fig. 1, artemisia annua residue general flavone purified has to hydroxyl radical free radical (OH) to be removed
Ability, but overall elimination effect is less than citric acid and ascorbic acid, when artemisia annua residue general flavone purified mass concentration is by 0.
2 g/L bring up to 1 g/L, and the elimination efficiency of hydroxyl radical free radical improves very fast；When mass concentration is more than 1g/L, elimination efficiency
Without significant change.
To the inoxidizability of grease
Under anhydrous acidic conditions, the peroxide in grease make I- be quantitatively oxidized to I2, I2 and I- combined generation it is soluble in water
I3-, be compared using color and the standard iodine solution of I3- quantitative.Under 353 nm wavelength, I2 contents (ρ) are 0~100
There is good linear relationship in mg/L with absorbance (A), extractive of general flavone is to the inoxidizability of grease using general in the world
Baking oven reinforcing storage method:It is added in liquid grease with the flavones refined solution of appropriate mass concentration, is sufficiently stirred for, in insulating box
Storage, group is compared to be not added with any antioxidant, and different time is measured by sampling the peroxide value of grease.And Vitamin C is used respectively
Acid and citric acid solution determine peroxide value, the oxidation resistance of relatively more various antioxidants as stated above.
The making of content of iodine standard curve
In a series of mL colorimetric cylinders of dryings 10, the chloroform-glacial acetic acid solution of 4 mL, 0.12mL KI saturations are separately added into
Alkali lye, plus a pipe mix a pipe and then plus 3mL distilled water, then divide addition iodine titer 0.0,0.1,0.2,0.4,0.6,0.8,
1.0 mL.Then plus distilled water is to scale, jump a queue and shake up, stand about 5-10 min, after being layered, take supernatant and move into 1cm's
In quartz colorimetric utensil, reference is made with zero pipe, its absorbance is surveyed under 353 nm wavelength, it is bent to make standard according to corresponding concentration
Line.
Calibration curve equation：Y=234x+0.6609, R2=0.9886, y are I2 contents.
Specific experiment step is
1. the mixed liquor for adding 24 mL to be matched by V (oil): V (flavones refined solution)=5: 1 in 100 mL triangular flasks.40
Reacted in DEG C baking oven, 60 min of interval take testing sample 0.1mL in 15 mL colorimetric cylinders are dried, add chloroform-glacial acetic acid molten
The mL of liquid 4, mixing is stirred evenly, and adds KI saturations alkali lye 0.12 mL, the s of jog 30, puts the min of dark place 3.
2. then add distilled water to scale, jump a queue, overturn, mix 2~3 times, stand about 5~10 min, treat that water is mutually clarified
Afterwards, supernatant is taken in 1 cm quartz colorimetric utensils, reference is made with blank at 353 nm, read the absorbance A of each pipe and count
Iodine growing amount is calculated, peroxide value (POV/%) is calculated by formula.
POV/%=（I2 contents (μ g)/sample size 0.086g)）×100
As a result record sheet
Peroxide value curve map is referring to Fig. 2.As can be seen from Figure 2, after addition flavones, peroxide value has different degrees of decline,
Illustrate that oxidation of the flavones to grease has different degrees of inhibitory action.And the flavones of 1g/L shows to the inoxidizability of animal fat
Write is higher than citric acid and ascorbic acid.
To the inhibition of tyrosinase vigor（That is whitening function）
Melanin（melanin）The main melanocyte by human body skin basal layer of epidermis（melanocyte）Produce, its energy
Reduce injury of the ultraviolet to skin.However, melanin can cause hyperpigmentation to cause black in the abnormal accumulation of basalis
Pinta, freckle, senile plaque expelling etc., influence the quality of life of people.With deepening continuously for studying melanin biosynthesis, research
Person has found that tyrosinase is played an important role in melanin biosynthesis, and it is tyrosine and DOPA to melanin transition process
In major rate-limiting enzyme, its overexpression is the main cause of hyperpigmentation due to amiodarone.Therefore, the work of tyrosinase is suppressed
Property can block the biosynthesis reaction chain of melanin, reduce the generation of melanin, realize the effect of whitening.This experiment is by surveying
Determine tyrosinase（MT）Catalysis L-3,4 dihydroxyphenylalanine（L-DOPA）DOPA quinone (the having characteristic absorption peak at 475 nm) of generation contains
Measure to evaluate the inhibitory action of flavones CE and other antioxidants to tyrosinase of artemisia annua residue.
Laboratory apparatus：Ultraviolet specrophotometer
Experiment reagent
The mother liquor of 10.0 mg/mL：Flavones refined solution, citric acid and the VC of 100.0 mg are taken, is settled to distilled water respectively
In 10 mL volumetric flasks, the mother liquor of 10.0 mg/mL is configured to.Used time is diluted to 0.2 with phosphate buffer solution, 0.4,0.6,
0.8、1.0mg/mL。
0.2 mol/L phosphate buffers (PBS）,pH6.8：2.84g disodium hydrogen phosphates are taken in small beaker, plus on a small quantity
Constant volume in distillation water dissolves, with 100mL volumetric flasks, is made the disodium phosphate soln of 0.2moL/L.Similarly take 2.6g di(2-ethylhexyl)phosphates
Hydrogen sodium is made into the sodium dihydrogen phosphate of 0.2moL/L.Take the disodium phosphate soln and 51mL0.2moL/L of 49mL0.2moL/L
Sodium dihydrogen phosphate mixing, adjust pH6.8.
1.0 mg/mL L-3,4 dihydroxyphenylalanine solution：10mg L-3,4 dihydroxyphenylalanines are weighed, is settled to 0.2 mol/L phosphate buffers
In 10 mL volumetric flasks, the L-3,4 dihydroxyphenylalanine solution of 1.0 mg/mL is configured to.
186 U/mL tyrosinases：Appropriate enzyme preparation is weighed, with 0.2 mol/L phosphate buffered salines into 186 U/
mL。
Experimental implementation table
In the dry colorimetric cylinders of 10mL, by sequentially adding various reagents in table, 4 groups of suctions in measurement each time at 475nm
Light Value Data, respectively A1, A2, A3, A4.
Increase by 0.001 for 1 enzyme activity unit with A475 per minute, the speed of enzymatic reaction is increased with A475 per minute
It is value added to represent.Measured since well mixed, determined once within every 30 seconds, measure 7 min, when A475 tends towards stability, its number
Value is exactly the A475 of the group.Enzyme activity and enzyme inhibition rate are calculated by following two formula.
Enzyme activity r=[（A3-A4）/（A1-A2）]×100%
Enzyme inhibition rate R=（1-r）×100%
Fig. 3 is the inhibition curve map of TYR enzyme, from the figure 3, it may be seen that artemisia annua flavones has necessarily to tyrosinase activity
Inhibition, when its concentration is in 0.2 ~ 0.4mg/mL, with the increase of concentration, inhibition is more obvious, and more than lemon
Acid and ascorbic acid, when concentration is more than 0.4mg/mL, inhibition constantly declines, but is consistently higher than citric acid.
The extracting method of volatile oil is：
By above-mentioned steps 2）Extract the artemisia annua residue powder after general flavone and press 1g:The solid-liquid ratio of 10ml adds n-hexane, is put into perseverance
In warm agitator, reflux condensing tube is connected, regulation rotating speed is 60r/s, and temperature is 75 DEG C, refluxing extraction 3 hours.To be flowed back immersion liquid
Collected by suction, repeats to extract artemisia annua residue, and backflow carries out volatilization concentration after taking out immersion liquid mixing.Be evaporated completely that n-hexane obtains waves
Hair oil crude product contains more impurity, and such as grease, wax, chlorophyll is extracted is dissolved in volatile oil in the lump, need to enter
One one-step refining is purified, and by volatile oil crude product anhydrous alcohol solution, sealing is placed on -20 DEG C of refrigerator overnights, and next day suction filtration is removed
Precipitate is removed, ethanol extract is collected, vacuum distillation removes ethanol, obtains blackish green volatile oil（Chlorophyll is not removed）.
Fungistatic effect to gained volatile oil is tested：
（1）By the corresponding liquid and solid medium of recipe configuration strain, draw 5 mL/ branch with liquid-transfering gun and load test tube,
121 DEG C of high-pressure steam sterilizing pans sterilizing 20min, put constant incubator into after cooling, 37 DEG C of bacterium, 28 DEG C of fungi, culture two is arrived
Three days, see whether complete sterilizing.
（2）Inoculation：A small amount of staphylococcus aureus, Escherichia coli, streptococcus are aseptically seeded to equipped with ox
The test tube of meat extract peptone nutrient solution, Candida albicans is inoculated in potato culture medium, and Penicillium notatum is inoculated in czapek's medium.It is every kind of
2 test tubes of microbionation, slight oscillatory test tube shakes up.Suitable constant incubator culture is placed in, it is fully bred, it is standby
With.
（3）Clean filter paper is made the roundlet scraps of paper of a diameter of 0.5cm with card punch, after hot air sterilization, difference is dipped in
In the volatile oil extracting liquid of concentration, fully absorb it, it is standby.
（4）Well-grown bacteria suspension after 0.5 mL transfers is drawn with liquid-transfering gun in super-clean bench, corresponding bacterium is injected and is put down
Plate, coating is uniform.The filter paper that immersion treatment is crossed is drained, is attached on above-mentioned flat board containing bacterium, each flat board pastes 3 identical Huangs
The filter paper of flower wormwood artemisia volatile oil mass concentration.Using acetone as blank, experiment 2 times is repeated.It is placed in incubator, bacterium 37
DEG C culture 24h, 28 DEG C of fungi culture 48h.Taking-up measures antibacterial circle diameter.Analysis fungistatic effect.
, referring to Fig. 4, as shown in Figure 4, artemisia annua residue volatile oil is to 5 kinds selected by experiment for the fungistatic effect figure of volatile oil
Bacterium has certain inhibition.Inhibition wherein to Penicillium notatum is optimal, and with the increase of concentration, effect is better；It is right
The inhibition change of other 4 kinds of bacterium is not obvious.
The preparation of shower cream：
It is 0.5% ~ 2% to set the flavones of artemisia annua residue extraction and its addition scope of volatile oil, takes 1%, 1.5%, 2% 3 ladder
Spend anti-oxidant experiment.Wherein, the flavones and its volatile oil proportioning that artemisia annua residue is extracted are as follows：
The quality of three volatile oil oil+flavones of gradient is as follows：
It is in proportion C12：C14=2：1 weighs 7.5 g fatty acid mixeds, is placed in small beaker, and 2.3 g potassium hydroxide are dissolved into
The aqueous solution of 50 %, after fatty acid mixed is mixed with a certain amount of deionized water during standby preparation, is heated to 80 DEG C, plus people
50 % potassium hydroxide solutions carry out saponification.After the active ingredient that above three gradient is added after fully reaction, distillation is added
Water is standby after fully mixing to 50mL.Shower cream is determined to the detection of its stability, inoxidizability, and its active effect
Optimum formula, and whitening effect is measured, and determines its optimum formula.
Stability Determination
During the sealing of above-mentioned shower cream preservative film is placed in into 0 DEG C and 50 DEG C of insulating boxs, its precipitation and layering feelings are observed every three days
Condition, the time occurred according to precipitation and delamination judges its stability, Continuous Observation 10 days.
Stability record sheet
The elimination effect of hydroxy radical
Record sheet（Control group has returned to zero）
Each experimental group to the clearance rate curve map of hydroxy radical referring to Fig. 5, as shown in Figure 5, single active ingredient flavones or volatilization
Oil is not obvious to the elimination effect of hydroxy radical, but the effect of blending constituent is generally better than single component.And ought be mixed into
Divide ratio in 60% ~ 80% volatile oil, during 20% ~ 40% flavones, its effect is most obvious, and elimination efficiency peaks.
Whitening effect
Experimental implementation table
In the dry colorimetric cylinders of 10mL, by sequentially adding various reagents in table, 4 groups of suctions in measurement each time at 475nm
Light Value Data, respectively A1, A2, A3, A4.
Increase by 0.001 for 1 enzyme activity unit with A475 per minute, the speed of enzymatic reaction is increased with A475 per minute
It is value added to represent.Measured since well mixed, determined once within every 30 seconds, measure 7 min, when A475 tends towards stability, its number
Value is exactly the A475 of the group.Enzyme activity and enzyme inhibition rate are calculated by following two formula.
Enzyme activity r=[（A3-A4）/（A1-A2）]×100%
Enzyme inhibition rate R=（1-r）×100%
Record sheet（A4 controls have been returned to zero）
Each experimental group to the inhibiting rate curve map of tyrosinase referring to Fig. 6, as shown in fig. 6, single active ingredient flavones or volatilization
Oil is not obvious to the inhibition of tyrosinase, but the effect of blending constituent is substantially better than single component.And ought be mixed into
Divide ratio at 40% ~ 60%, its effect is most obvious, and elimination efficiency peaks.
Embodiment described above only expresses specific embodiment of the invention, and its description is more specific and detailed, but simultaneously
Can not therefore be construed as limiting the scope of the patent.It should be pointed out that coming for one of ordinary skill in the art
Say, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection of the invention
Scope.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (9)
1. the washing product composition of remaining artemisia annua residue preparation after qinghaosu is extracted in a kind of utilization, it is characterised in that contained
Have：Using the flavones for extracting the preparation of the artemisia annua residue after qinghaosu；The artemisia annua residue after qinghaosu and flavones is extracted successively
The volatile oil obtained with Solvent Extract methods；And washing product auxiliary material.
2. the washing product combination of remaining artemisia annua residue preparation after qinghaosu is extracted in utilization according to claim 1
Thing, it is characterised in that：The flavones is 20 ~ 40 with the mass ratio of volatile oil：60~80.
3. the washing product combination of remaining artemisia annua residue preparation after qinghaosu is extracted in utilization according to claim 1
Thing, it is characterised in that：The flavones accounts for the 1% ~ 1.5% of washing product composition weight with the gross mass of volatile oil.
4. the washing product combination of remaining artemisia annua residue preparation after qinghaosu is extracted in utilization according to claim 1
Thing, it is characterised in that the extracting method of the flavones is：
（1）The constant temperature in 60 DEG C of baking ovens of the artemisia annua residue after qinghaosu will be extracted to dry to constant weight, crushing in pulverizer will be put into,
Sieving, sealing preserve is standby；
（2）By step（1）Residue obtained powder ethanol solution is aided with ultrasonic wave extraction, artemisia annua residue is obtained after filtering total
Flavone extractive；
（3）Step（2）Resulting material concentration removal ethanol, centrifugation takes supernatant, obtains flavones crude extract after going the removal of impurity；
（4）Step（3）Gained flavones crude extract is purified using macroporous adsorbent resin column chromatography method, obtains flavones.
5. the washing product combination of remaining artemisia annua residue preparation after qinghaosu is extracted in utilization according to claim 4
Thing, it is characterised in that：Step（2）Residue powder is 1g with the solid-liquid ratio of ethanol solution:10 ~ 40 mL, the volume of ethanol solution
Percentage is 30% ~ 60%, and extraction time is 20 ~ 50min, and Extracting temperature is 40 ~ 60 DEG C, and ultrasonic power is 300W.
6. the washing product combination of remaining artemisia annua residue preparation after qinghaosu is extracted in utilization according to claim 5
Thing, it is characterised in that：Step（2）Residue powder is 1g with the solid-liquid ratio of ethanol solution:40 mL, the volume basis of ethanol solution
Than being 40%, extraction time is 20min, and Extracting temperature is 60 DEG C, and ultrasonic power is 300W.
7. the washing product combination of remaining artemisia annua residue preparation after qinghaosu is extracted in utilization according to claim 4
Thing, it is characterised in that the extracting method of the volatile oil is：It is residual using the artemisia annua extracted after qinghaosu extracted after general flavone
Slag, uses organic solvent reflux extraction, obtains backflow and takes out immersion liquid, and volatile oil is obtained after volatilization concentration, refined purification.
8. the washing product combination of remaining artemisia annua residue preparation after qinghaosu is extracted in utilization according to claim 7
Thing, it is characterised in that the extracting method of the volatile oil is：It is residual using the artemisia annua extracted after qinghaosu extracted after general flavone
Slag, by solid-liquid ratio 1g：The ratio of 10ml adds n-hexane, is put into thermostatic mixer, connects reflux condensing tube, and regulation rotating speed is
60r/s, temperature is 75 DEG C, refluxing extraction 3 hours, and the immersion liquid collected by suction that flows back repeats to extract artemisia annua residue, and leaching is taken out in backflow
Volatilization concentration is carried out after liquid mixing, and uses anhydrous alcohol solution, sealing is placed on -20 DEG C of refrigerator overnights, next day suction filtration is removed
Precipitate, collects ethanol extract, and vacuum distillation removes ethanol, obtains blackish green volatile oil.
9. the washing product combination of remaining artemisia annua residue preparation after qinghaosu is extracted in utilization according to claim 1
Thing, it is characterised in that：The washing product composition is shower cream.
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