CN101982183A - Preparation method of Astilbe chinensis root extract and antioxidant application of Astilbe chinensis root extract - Google Patents
Preparation method of Astilbe chinensis root extract and antioxidant application of Astilbe chinensis root extract Download PDFInfo
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Abstract
The invention belongs to the technical field of researching and developing medical products, and relates to a preparation method of an Astilbe chinensis root extract and antioxidant application of the Astilbe chinensis root extract. The preparation method comprises the following steps: lixiviating the taken Astilbe chinensis root powder with 70% ethanol for 8h-10h at room temperature; standing and filtering; then respectively utilizing 8 times amount of the 70% ethanol and 6 times amount of the 70% ethanol to process the filter residue by repeating the above steps once, and carrying out ultrasonic processing for 30min at the temperature of 30 DEG C and the power of 80W; merging the filtrates, and recycling solvent, thus obtaining the total extract; suspending with water, and extracting the suspension with water saturated n-butyl alcohol for three times; merging n-butyl alcohol layer extract liquor, and carrying out concentration at reduced pressure; and cooling and drying on the n-butyl alcohol layer extract liquor, thus obtaining the Astilbe chinensis root n-butyl alcohol layer extract. The Astilbe chinensis root n-butyl alcohol layer extract has strong removal effect on ABTS<+> radicals and DPPH radicals, can be developed into natural antioxidant drugs to substitute for artificially synthesized antioxidant to treat diseases caused by excessive radicals in human bodies.
Description
Technical field
Medical product research and development technical field under the present invention.The present invention relates to a kind of Radix astilbes chinensis (Rhizoma Astilbes Chinensis) preparation method of extract and antioxidation purposes thereof.
Background technology
Herba astilbes chinensis Astilbe chinensis (Maxim.) Franch.et Sav is the Saxifragaceae astilbe, and its acrid in the mouth, hardship are warm in nature.This plant roots and stems is used as medicine, and is widely used among the people, is mainly used in treatment traumatic injury, rheumatic arthritis, anemopyretic cold, venom etc.Herba astilbes chinensis is widely distributed in China the Northeast, and aboundresources is gathered easily, and the using value height, has great potentiality to be exploited.Free radical is the active chemical group that a class has unpaired electronics.Think at present, human diseases such as various inflammation, atherosclerosis, presenile dementia, parkinson, acute cerebrovascular disease, cancer, aging etc., all the oxidation with free radical stimulates closely related.Because the mechanism of oxidative damage plays an important role in the developing of disease, therefore, the antioxidation of Chinese medicine is its key factor that reaches therapeutic effect.Discover that the antioxidant of current main use mostly is the synthetic antioxidant, there are many negative effects in its health to the people.Therefore, in recent years, academia grows with each passing day to the research interest of the Natural antioxidant that exists in the plant.In order to seek antioxidant efficiently, the research of the Natural antioxidant is seemed extremely important.Antioxidative research has at present become focus, but the antioxidant activity of Radix astilbes chinensis (Rhizoma Astilbes Chinensis) extract is studied rarely seen report.
Summary of the invention
The present invention is intended to inquire into Radix astilbes chinensis (Rhizoma Astilbes Chinensis) preparation method of extract and uses thereof, for the exploitation Natural antioxidant of fields such as medical science, Food Science provides new resources.
1, a kind of Radix astilbes chinensis (Rhizoma Astilbes Chinensis) preparation method of extract is characterized in that may further comprise the steps:
(1) gets Radix astilbes chinensis (Rhizoma Astilbes Chinensis) 40 order powder, doubly measure lixiviate 8h-10h under the 70% ethanol room temperature, leave standstill filtration with 6-8;
(2) filtering residue is repeated above step respectively once with the ethanol of 8 times of amounts and 6 times of amounts 70% respectively again, 30 ℃, 80W, ultrasonic 30min;
(3) merging filtrate reclaims solvent and promptly gets total extract;
(4) with total extract water suspendible in (three), the water saturated n-butyl alcohol of reuse is to this suspension extraction 3 times;
(5) merge the n-butanol layer extract;
(6) with n-butanol layer extract concentrating under reduced pressure in (five), lyophilization obtains Radix astilbes chinensis (Rhizoma Astilbes Chinensis) n-butanol layer extract.
2, the described Radix astilbes chinensis (Rhizoma Astilbes Chinensis) preparation method of extract of claim 1 is characterised in that its raw material is the root of Herba astilbes chinensis Astilbechinensis (Maxim.) Franch.et Sav.
3, a kind of antioxidation purposes of Radix astilbes chinensis (Rhizoma Astilbes Chinensis) extract is characterized in that this Radix astilbes chinensis (Rhizoma Astilbes Chinensis) extract is to ABTS
+Free radical and DPPH free radical have very strong scavenging action.
4, a kind of antioxidation purposes of Radix astilbes chinensis (Rhizoma Astilbes Chinensis) extract is characterized in that this Radix astilbes chinensis (Rhizoma Astilbes Chinensis) extract can be developed as the natural anti-oxidation medicine, the disease that the antioxidant therapy of replacement synthetic too much produces because of free radical in the human body.
5, the antioxidation purposes of claim 3,4 each described Radix astilbes chinensis (Rhizoma Astilbes Chinensis) extracts is characterised in that its preparation raw material is the root of Herba astilbes chinensis Astilbe chinensis (Maxim.) Franch.et Sav.
According to the present invention, " % " among the present invention is percentage by weight.
The specific embodiment
Of the present inventionly relate to a kind of Radix astilbes chinensis (Rhizoma Astilbes Chinensis) preparation method of extract and the antioxidation purposes comprises following examples, the following examples can further specify the present invention, but do not limit the present invention in any way.
Embodiment 1:
(1) gets Radix astilbes chinensis (Rhizoma Astilbes Chinensis) 40 order powder 100g,, leave standstill filtration with lixiviate 8h under 6 times of amount 70% ethanol room temperatures;
(2) filtering residue is repeated above step respectively once with the ethanol of 8 times of amounts and 6 times of amounts 70% respectively again, 30 ℃, 80W, ultrasonic 30min;
(3) merging filtrate reclaims solvent and promptly gets total extract;
(4) with total extract water suspendible in (three), the water saturated n-butyl alcohol of reuse is to this suspension extraction 3 times;
(5) merge the n-butanol layer extract;
(6) with n-butanol layer extract concentrating under reduced pressure in (five), lyophilization obtains Radix astilbes chinensis (Rhizoma Astilbes Chinensis) n-butanol layer extract;
(7) n-butanol layer extract in (six) is carried out the antioxidation test, this extract is to ABTS
+Free radical and DPPH free radical have very strong scavenging action.
Embodiment 2:
(1) gets Radix astilbes chinensis (Rhizoma Astilbes Chinensis) 40 order powder 200g,, leave standstill filtration with lixiviate 10h under 8 times of amount 70% ethanol room temperatures;
(2) filtering residue is repeated above step respectively once with the ethanol of 8 times of amounts and 6 times of amounts 70% respectively again, 30 ℃, 80W, ultrasonic 30min;
(3) merging filtrate reclaims solvent and promptly gets total extract;
(4) with total extract water suspendible in (three), the water saturated n-butyl alcohol of reuse is to this suspension extraction 3 times;
(5) merge the n-butanol layer extract;
(6) with n-butanol layer extract concentrating under reduced pressure in (five), lyophilization obtains Radix astilbes chinensis (Rhizoma Astilbes Chinensis) n-butanol layer extract;
(7) n-butanol layer extract in (six) is carried out the antioxidation test, this extract is to ABTS
+Free radical and DPPH free radical have very strong scavenging action.
Pharmacological test example: the antioxidant activity of Radix astilbes chinensis (Rhizoma Astilbes Chinensis) extract
1 materials and methods
1.1 laboratory sample source
Medical material picks up from Jilin Province near a river, is accredited as the root of Herba astilbes chinensis Astilbe chinensis (Maxim.) Franch.et Sav by professor Hu Quande of gardening institute of Jilin Agriculture University.
1.2 instrument
76 new century ultraviolet-uisible spectrophotometers (Beijing Puxi General Instrument Co., Ltd); Lark LA114 type electronic balance (110g/0.0001g) (Bailing Balance Instrument Co., Ltd., Changshu); Electronic counting balance (500g/0.001g) (golden Yang Tianpingyiqichang); KQ-250DB type ultrasonic cleaner (Kunshan Ultrasonic Instruments Co., Ltd.); 101-2A type digital display electric heating is advertised drying baker (silk screen instrument and meter company limited Tongzhou, Shanghai branch company); Rotary Evaporators (Shanghai Yarong Biochemical Instrument Plant); Liquid-transfering gun (20ul); Water-bath.
1.3 reagent
DPPH solution, distilled water, dehydrated alcohol, methanol, ABTS, potassium peroxydisulfate, the potassium ferricyanide, phosphate buffer (PH=6.6), trichloroacetic acid, ferric chloride, VC (vitamin C).
2 experimental techniques and step
2.1 the extraction of medical material component
Get Radix astilbes chinensis (Rhizoma Astilbes Chinensis) 40 order powder 200g,, leave standstill filtration with lixiviate 10h under 8 times of amount 70% ethanol room temperatures, filtering residue is repeated above step respectively once with the ethanol of 8 times of amounts and 6 times of amounts 70% respectively again, 30 ℃, 80W, ultrasonic 30min, merging filtrate reclaims solvent and promptly gets total extract, total extract water suspendible, the water saturated n-butyl alcohol of reuse merges the n-butanol layer extract, concentrating under reduced pressure to this suspension extraction 3 times, lyophilization obtains Radix astilbes chinensis (Rhizoma Astilbes Chinensis) n-butanol layer extract 5.19g.
2.2ABTS method is measured antioxidant activity
2.2.1 the preparation of sample solution
Accurately take by weighing the n-butanol layer extract 30mg that is dried to constant weight, the water standardize solution is in the 10mL volumetric flask, being made into concentration is the sample mother solution of 3mg/mL, it is 0.018mg/mL that sample mother solution water stepwise dilution is become concentration, 0.037mg/mL, 0.075mg/mL, 0.15mg/mL, 0.31mg/mL, the sample solution of 0.62mg/mL, standby.
2.2.2ABTS
+.The preparation of solution
5mL, the ABTS of 14mM and 5mL, 4.9mMk
2S
2O
8Mix and produce ABTS
+., static 16h in the dark uses the ethanol dilution working solution before using, and requiring its absorbance under the 734nm wavelength is 0.70 ± 0.02.
2.2.3 sample determination
Get the 3mL sample solution and add 158 μ LABTS
+.(A
Sample), do blank with ethanol, 158 μ LABTS
+.With 3mL water be titer (A
Control), under the 734nm wavelength, measure absorbance behind the reaction 6min, compare with VC (vitamin C).
Clearance rate (%)=[(A
Control-A
Sample)/A
Contro1] * 100%
2.3DPPH method is measured antioxidant activity
2.3.1 the preparation of sample solution
Accurately take by weighing the n-butanol layer extract 30mg that is dried to constant weight, with the dehydrated alcohol standardize solution in the 10mL volumetric flask, being made into concentration is the sample mother solution of 3mg/mL, it is 0.018mg/mL that the sample mother solution is become concentration with the dehydrated alcohol stepwise dilution, 0.037mg/mL, 0.075mg/mL, 0.15mg/mL, 0.31mg/mL, the sample solution of 0.62mg/mL, standby.
2.3.2DPPH the preparation of solution
Precision takes by weighing DPPH sample 0.01g, adds dehydrated alcohol and is settled in the 50mL volumetric flask, and obtaining concentration is the DPPH mother solution of 0.2mg/mL.Pipette 10mL from mother solution, be settled in the 50mL volumetric flask with dehydrated alcohol, obtaining concentration is the DPPH solution of 0.04mg/mL, and lucifuge is standby.
2.3.3 sample determination
Extracting sample solution is pressed table 1 and is added reactant liquor, shakes up the back and leaves standstill 30min in the room temperature lucifuge, measures absorbance at the 515nm place.Compare with VC (vitamin C).
Table 1 n-butanol layer extract sample solution application of sample table
DPPH clearance rate %=(A-B+C)/A*100
3 results and discussion
3.1 result
3.1.1ABTS method is removed the measurement result of free radical effect, sees Table 2.
Radix astilbes chinensis (Rhizoma Astilbes Chinensis) n-butanol layer extract solution ABTS method is removed free radical effect (clearance rate %) under table 2 variable concentrations
| Concentration (mg/mL) | VC | N-butanol layer |
| 0.018 | 42.66 | 25.89 |
| 0.037 | 57.95 | 38.24 |
| 0.075 | 81.72 | 57.61 |
| 0.150 | 95.73 | 78.72 |
| 0.310 | 98.62 | 96.43 |
| 0.620 | 99.69 | 99.12 |
3.1.2DPPH method is removed the measurement result of free radical ability, sees Table 3.
Radix astilbes chinensis (Rhizoma Astilbes Chinensis) n-butanol layer extract solution DPPH method is removed free radical effect (clearance rate %) under table 3. variable concentrations
| Concentration (mg/mL) | VC | N-butanol layer |
| 0.018 | 81.72 | 78.12 |
| 0.037 | 86.95 | 85.54 |
| 0.075 | 90.36 | 91.72 |
| 0.150 | 94.18 | 95.67 |
| 0.310 | 95.46 | 96.38 |
| 0.620 | 96.38 | 98.34 |
3.2 discuss
3.2.1 as shown in Table 2: Radix astilbes chinensis (Rhizoma Astilbes Chinensis) n-butanol layer extract is to ABTS
+Free radical has very strong scavenging action, when sample solution concentration is 0.620mg/mL, and the removing ABTS of n-butanol layer extract
+The free radical ability is equal to VC.
3.2.2 as shown in Table 3: Radix astilbes chinensis (Rhizoma Astilbes Chinensis) n-butanol layer extract has very strong scavenging action to the DPPH free radical, is 0.075mg/mL when above in sample solution concentration, and the removing DPPH free radical rate of n-butanol layer extract is greater than VC.
The Herba astilbes chinensis antioxidation is very strong, if it can be developed to the Natural antioxidant, puts into production, and the antioxidant therapy that both can replace synthetic also can be enterprise and brings considerable economic because of the disease that free radical in the human body too much produces.
Claims (5)
1. Radix astilbes chinensis (Rhizoma Astilbes Chinensis) preparation method of extract is characterized in that may further comprise the steps:
(1) gets Radix astilbes chinensis (Rhizoma Astilbes Chinensis) 40 order powder, doubly measure lixiviate 8h-10h under the 70% ethanol room temperature, leave standstill filtration with 6-8;
(2) filtering residue is repeated above step respectively once with the ethanol of 8 times of amounts and 6 times of amounts 70% respectively again, 30 ℃, 80W, ultrasonic 30min;
(3) merging filtrate reclaims solvent and promptly gets total extract;
(4) with total extract water suspendible in (three), the water saturated n-butyl alcohol of reuse is to this suspension extraction 3 times;
(5) merge the n-butanol layer extract;
(6) with n-butanol layer extract concentrating under reduced pressure in (five), lyophilization obtains Radix astilbes chinensis (Rhizoma Astilbes Chinensis) n-butanol layer extract.
2. the described Radix astilbes chinensis (Rhizoma Astilbes Chinensis) preparation method of extract of claim 1 is characterised in that its raw material is the root of Herba astilbes chinensis Astilbechinensis (Maxim.) Franch.et Sav.
3. the antioxidation purposes of a Radix astilbes chinensis (Rhizoma Astilbes Chinensis) extract is characterized in that this Radix astilbes chinensis (Rhizoma Astilbes Chinensis) extract is to ABTS
+Free radical and DPPH free radical have very strong scavenging action.
4. the antioxidation purposes of a Radix astilbes chinensis (Rhizoma Astilbes Chinensis) extract is characterized in that this Radix astilbes chinensis (Rhizoma Astilbes Chinensis) extract can be developed as the natural anti-oxidation medicine, the disease that the antioxidant therapy of replacement synthetic too much produces because of free radical in the human body.
5. the antioxidation purposes of claim 3,4 each described Radix astilbes chinensis (Rhizoma Astilbes Chinensis) extracts is characterised in that its preparation raw material is the root of Herba astilbes chinensis Astilbe chinensis (Maxim.) Franch.et Sav.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105708873A (en) * | 2016-04-19 | 2016-06-29 | 建德市大洋南山中药材专业合作社 | Chinese astilbe herb ultra-fine powder decoction pieces and preparation method thereof |
| CN106138127A (en) * | 2016-08-15 | 2016-11-23 | 锦州医科大学 | There is gland calyx Herba astilbes chinensis extract and the application thereof improving and preventing and treating obesity effect |
| CN106421075A (en) * | 2016-12-05 | 2017-02-22 | 山东省科学院生物研究所 | Preparation method and application of anti-oxidation active component of bamboo willows |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100348606C (en) * | 2005-07-08 | 2007-11-14 | 南京大学 | Preparation method of astilbin |
-
2010
- 2010-10-18 CN CN 201010519687 patent/CN101982183A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100348606C (en) * | 2005-07-08 | 2007-11-14 | 南京大学 | Preparation method of astilbin |
Non-Patent Citations (2)
| Title |
|---|
| 《中国中药杂志》 20040730 陈浩等 落新妇的成分研究（Ⅰ） 652-654 1-5 第29卷, 第7期 2 * |
| 《中国博士学位论文全文数据库工程科技Ⅰ辑》 20070531 胡君一 药用植物青刺尖和落新妇生物活性成分的研究 B016-18 1-2 , 第5期 2 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105708873A (en) * | 2016-04-19 | 2016-06-29 | 建德市大洋南山中药材专业合作社 | Chinese astilbe herb ultra-fine powder decoction pieces and preparation method thereof |
| CN106138127A (en) * | 2016-08-15 | 2016-11-23 | 锦州医科大学 | There is gland calyx Herba astilbes chinensis extract and the application thereof improving and preventing and treating obesity effect |
| CN106421075A (en) * | 2016-12-05 | 2017-02-22 | 山东省科学院生物研究所 | Preparation method and application of anti-oxidation active component of bamboo willows |
| CN106421075B (en) * | 2016-12-05 | 2019-10-15 | 山东省科学院生物研究所 | A kind of preparation method and application of bamboo willow antioxidant activity component |
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Open date: 20110302 |