CN110438007A - 多细胞体系培养瓶 - Google Patents

多细胞体系培养瓶 Download PDF

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CN110438007A
CN110438007A CN201910675850.4A CN201910675850A CN110438007A CN 110438007 A CN110438007 A CN 110438007A CN 201910675850 A CN201910675850 A CN 201910675850A CN 110438007 A CN110438007 A CN 110438007A
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many cells
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王智文
汪保卫
陈涛
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Tianjin University
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Abstract

本发明公开了一种多细胞体系培养瓶，由培养瓶体和盖子组成，分隔膜组件将培养瓶体内空间分隔设置成2‑4个区室，所述分隔膜组件由位于上部的非通透性膜和位于中下部的微孔滤膜组成。本发明的多细胞体系培养瓶可方便进行二、三或四细胞体系培养，并且培养体系内溶液可自由扩散，方便营养及代谢物质交换、同时还具有使用方便、适用范围广、生产制造成本低廉等特点，便于多个生物学样本的平行实验研究。

Description

多细胞体系培养瓶
发明领域
本发明涉及生物细胞培养领域，特别是涉及多细胞体系培养瓶。
背景技术
纯种培养技术是生物学研究的一项关键技术，纯种培养技术的发明使得对特定生物学过程的细胞生物学、生理学、生物化学与分子生物学研究成为可能，如酿酒酵母发酵生产酒精的过程，因此一大批与之相适应的纯种培养技术包括培养基设计、无菌培养操作、灭菌方法以及相应的生物反应器设计等等得到了充分快速的发展(诸葛健,1994)。然而，在自然环境中，细胞的生长更多的是处于一个多细胞体系的环境中，多细胞体系的培养成为研究更广泛的生物学过程的一种不二之选(Fu et al.,2019；Hoshino,Onaka,&Abe,2019；Islam,Ong,Ethiraj,Cheng,&Rahman Khan,2018；Li,Wang,&Zhang,2019；Reis et al.,2018； Thuan,Chaudhary,VanCuong,&Cuong,2018；Tognon,Kohler,Luscher,&van Delden,2019)。近年来多细胞体系培养研究也成为生物学与医学研究的热点问题，与之不相协调的是目前用于多细胞体系培养的技术体系仍不够丰富和成熟，存在相应的培养过程难以实现或培养状态控制不理想等问题。
因此，亟需一种能够很方便的进行多细胞体系培养的多细胞体系培养瓶，用于多细胞体系的培养研究。
发明内容
本发明的目的是克服现有技术的不足，提供一种适用于多细胞体系培养的研究与应用，从而方便多细胞体系的研究开发的多细胞体系培养瓶。
本发明的技术方案概述如下：
一种多细胞体系培养瓶，由培养瓶体和盖子组成，分隔膜组件将培养瓶体内空间分隔设置成2-4个区室，所述分隔膜组件由位于上部的非通透性膜和位于中下部的微孔滤膜组成。
培养瓶体呈圆柱型或圆台型。
优选地，非通透性膜的高等于分隔膜组件高的10％。
优选地，分隔膜组件将培养瓶内空间分隔设置为沿培养瓶体的轴向分割设置。
优选地，微孔滤膜的孔径为小于等于0.22μm。
所述盖子是带有透气性聚合物膜内衬的有孔盖或不透气的盖。
本发明的多细胞体系培养瓶可方便进行二、三或四细胞体系培养，并且培养体系内溶液可自由扩散，方便营养及代谢物质交换、同时还具有使用方便、适用范围广、生产制造成本低廉等特点，便于多个生物学样本的平行实验研究。
附图说明
图1为多细胞体系培养瓶结构示意图。
图2为图1的多细胞体系培养瓶过A-A剖面瓶体被分隔膜组件分隔成2个区室示意图。
图3为瓶体被分隔膜组件分隔成4个区室示意图。
图4为瓶体被分隔膜组件分隔成3个区室示意图。
图5为另一种多细胞体系培养瓶结构示意图。
具体实施方式
下面结合附图和具体实施方式对本发明进一步说明。
一种多细胞体系培养瓶(见图1、2、3、4)，由培养瓶体1和盖子2组成，分隔膜组件3将培养瓶体内空间分隔设置成2个区室，也可以分隔设置成3个区室或4个区室，分隔膜组件由位于上部的非通透性膜4和位于中下部的微孔滤膜5组成。
培养瓶体呈圆柱型或圆台型(见图5)。
非通透性膜4的高等于分隔膜组件3高的10％。
分隔膜组件将培养瓶内空间分隔设置为沿培养瓶体的轴向分割设置。
微孔滤膜的孔径大小可根据培养细胞要求选定，小于等于0.22μm，例如0.22μm，0.20μm。
该分离膜可实现培养瓶内部培养液的均匀透过，而阻隔细胞的通透。所述分隔膜组件和培养瓶体无缝嵌合连接。
所述盖子是带有透气性聚合物膜内衬的有孔盖或不透气的盖。盖的材料可以选用塑料。
本发明的多细胞体系培养瓶可方便进行二、三或四细胞体系培养，并且培养体系内溶液可自由扩散，方便营养及代谢物质交换、同时还具有使用方便、适用范围广、生产制造成本低廉等特点，便于多个生物学样本的平行实验研究。
多细胞体系培养瓶及其盖子可以进行高温蒸汽，辐射，熏蒸等方式灭菌。
多细胞体系培养瓶的使用方法：
向多细胞体系培养瓶中加入所需培养基，经高温蒸汽灭菌，冷却后；通过无菌操作方法分别向不同的区室中接种入目标细胞。盖好盖子，放入到37℃恒温摇床中220rpm振荡培养，以一定时间间隔从各区室中吸取1ml培养液，测定细胞浓度。所述细胞培养液还可以进一步进行细胞学、转录组、代谢物组等系统生物学分析。
应理解的是，以上所述仅为本发明的较佳实施例而已，并不用以限制本发明，凡在本发明的精神和原则之内，所作的任何修改、等同替换、改进等，均应包含在本发明的保护范围之内。
参考文献:
Fu,L.,Zhang,F.,Bai,Y.N.,Lu,Y.Z.,Ding,J.,Zhou,D.,Liu,Y.,Zeng,R.J.(2019).Mass transfer affects reactor performance,microbial morphology,andcommunity succession in the methane-dependent denitrification and anaerobicammonium oxidation co-culture.Sci Total Environ,651(Pt1),291-297.doi:10.1016/j.scitotenv.2018.09.184
Hoshino,S.,Onaka,H.,&Abe,I.(2019).Activation of silent biosyntheticpathways and discovery of novel secondary metabolites in actinomycetes by co-culture with mycolic acid- containing bacteria.J Ind Microbiol Biotechnol,46(3-4),363-374.doi:10.1007/s10295- 018-2100-y
Islam,M.A.,Ong,H.R.,Ethiraj,B.,Cheng,C.K.,&Rahman Khan,M.M.(2018).Optimization of co-culture inoculated microbial fuel cell performance usingresponse surface methodology.J Environ Manage,225,242-251.doi:10.1016/j.jenvman.2018.08.002
Li,Z.,Wang,X.,&Zhang,H.(2019).Balancing the non-linear rosmarinicacid biosynthetic pathway by modular co-culture engineering.Metab Eng.doi:10.1016/j.ymben.2019.03.002
Reis,V.R.,Bassi,A.P.G.,Cerri,B.C.,Almeida,A.R.,Carvalho,I.G.B.,Bastos,R.G.,& Ceccato-Antonini,S.R.(2018).Effects of feedstock and co-cultureof Lactobacillus fermentum and wild Saccharomyces cerevisiae strain duringfuel ethanol fermentation by the industrial yeast strain PE-2.AMB Express,8(1),23.doi:10.1186/s13568-018-0556-9
Thuan,N.H.,Chaudhary,A.K.,Van Cuong,D.,&Cuong,N.X.(2018).Engineeringco-culture system for production of apigetrin in Escherichia coli.J IndMicrobiol Biotechnol,45(3), 175-185.doi:10.1007/s10295-018-2012-x
Tognon,M.,Kohler,T.,Luscher,A.,&van Delden,C.(2019).Transcriptionalprofiling of Pseudomonas aeruginosa and Staphylococcus aureus during in vitroco-culture.BMC Genomics,20(1),30.doi:10.1186/s12864-018-5398-y
诸葛健.(1994).工业微生物实验技术手册:中国轻工业出版社。

Claims (6)

1.一种多细胞体系培养瓶，由培养瓶体和盖子组成，其特征是分隔膜组件将培养瓶体内空间分隔设置成2-4个区室，所述分隔膜组件由位于上部的非通透性膜和位于中下部的微孔滤膜组成。
2.根据权利要求1所述的一种多细胞体系培养瓶，其特征是所述培养瓶体呈圆柱型或圆台型。
3.根据权利要求1所述的一种多细胞体系培养瓶，其特征是所述非通透性膜的高等于分隔膜组件高的10％。
4.根据权利要求1所述的一种多细胞体系培养瓶，其特征是所述分隔膜组件将培养瓶内空间分隔设置为沿培养瓶体的轴向分割设置。
5.根据权利要求1所述的一种多细胞体系培养瓶，其特征是所述微孔滤膜的孔径小于等于0.22μm。
6.权利要求1所述的一种多细胞体系培养瓶，其特征是所述盖子是带有透气性聚合物膜内衬的有孔盖。
CN201910675850.4A 2019-07-25 2019-07-25 多细胞体系培养瓶 Pending CN110438007A (zh)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050148068A1 (en) * 2003-12-31 2005-07-07 Lacey William J. Cell cultivating flask
US20090298164A1 (en) * 2008-05-30 2009-12-03 Cattadoris Henry J Assembly of cell culture vessels
CN202849409U (zh) * 2012-10-23 2013-04-03 杭州赫贝科技有限公司 一种细胞共培养瓶
CN204644371U (zh) * 2015-02-05 2015-09-16 中山大学 一种集成式多细胞非接触共培养装置
CN108277158A (zh) * 2018-04-20 2018-07-13 协和华东干细胞基因工程有限公司 一种细胞培养瓶

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050148068A1 (en) * 2003-12-31 2005-07-07 Lacey William J. Cell cultivating flask
US20090298164A1 (en) * 2008-05-30 2009-12-03 Cattadoris Henry J Assembly of cell culture vessels
CN202849409U (zh) * 2012-10-23 2013-04-03 杭州赫贝科技有限公司 一种细胞共培养瓶
CN204644371U (zh) * 2015-02-05 2015-09-16 中山大学 一种集成式多细胞非接触共培养装置
CN108277158A (zh) * 2018-04-20 2018-07-13 协和华东干细胞基因工程有限公司 一种细胞培养瓶

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Application publication date: 20191112