CN110437993A - A kind of multiple physical field coupling bioreactor - Google Patents
A kind of multiple physical field coupling bioreactor Download PDFInfo
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- CN110437993A CN110437993A CN201910881952.1A CN201910881952A CN110437993A CN 110437993 A CN110437993 A CN 110437993A CN 201910881952 A CN201910881952 A CN 201910881952A CN 110437993 A CN110437993 A CN 110437993A
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- 230000008878 coupling Effects 0.000 title claims abstract description 13
- 238000010168 coupling process Methods 0.000 title claims abstract description 13
- 238000005859 coupling reaction Methods 0.000 title claims abstract description 13
- 239000011521 glass Substances 0.000 claims abstract description 36
- 238000001816 cooling Methods 0.000 claims abstract description 23
- 238000005286 illumination Methods 0.000 claims abstract description 17
- 239000000523 sample Substances 0.000 claims abstract description 4
- 239000000463 material Substances 0.000 claims description 3
- 239000002184 metal Substances 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 238000000338 in vitro Methods 0.000 claims description 2
- 238000012216 screening Methods 0.000 claims 1
- 244000005700 microbiome Species 0.000 abstract description 9
- 238000012423 maintenance Methods 0.000 abstract description 5
- 230000001954 sterilising effect Effects 0.000 abstract description 5
- 239000007788 liquid Substances 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 238000000855 fermentation Methods 0.000 description 3
- 230000004151 fermentation Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 108010053481 Antifreeze Proteins Proteins 0.000 description 1
- 102000003939 Membrane transport proteins Human genes 0.000 description 1
- 108090000301 Membrane transport proteins Proteins 0.000 description 1
- 230000002528 anti-freeze Effects 0.000 description 1
- 239000000110 cooling liquid Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/02—Means for providing, directing, scattering or concentrating light located outside the reactor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/06—Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
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- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
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- Bioinformatics & Cheminformatics (AREA)
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- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Sustainable Development (AREA)
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- General Health & Medical Sciences (AREA)
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Abstract
A kind of multiple physical field coupling bioreactor, biological respinse tank body, ultrasonic wave generation and control device, illumination generation and control device and magnetic field generation and control device including being sequentially communicated setting, magnetic field, which is generated, is connected to setting with control device with biological respinse tank body；It includes supersonic generator and container that ultrasonic wave, which is generated with control device, and the probe of supersonic generator is arranged in container, and the first cooling layer is provided with outside container；Illumination generation and control device include the first glass tube, are provided with shading sleeve outside the first glass tube, the inner wall of shading sleeve is provided with illuminator；It includes the second glass tube that magnetic field, which is generated with control device, and electromagnetic coil is provided with outside the second glass tube.The present invention can sufficiently cultivate different microorganisms, and individually sterilizing can be achieved in the cavity and pipeline to device each in system, and maintenance upgrade is easy, it can also be connect with any type tank body, for microorganism difference culture demand, use can be freely combined, it is applied widely.
Description
Technical field
The invention belongs to bioreaction technology fields, and in particular to a kind of multiple physical field coupling bioreactor.
Background technique
Bioreactor refers to and utilizes the microorganism of naturally occurring or the microbial inoculant with special fermenting property to liquid
The reaction system of phase or solid phase.
Traditional bioreactor is mostly integrated equipment, and fixation is not easy to remove between each cavity and pipeline, therefore right
The maintenance of equipment is also more troublesome, and sterilizing works are also relatively complicated, and relative cost is higher, and can only meet the micro- life in part
The culture demand of object, the scope of application are small.
Therefore, a kind of multiple physical field coupling bioreactor is devised to solve the above problems.
Summary of the invention
To overcome the deficiencies in the prior art described above, it is an object of that present invention to provide a kind of reactions of multiple physical field coupled biological
Device.
In order to achieve the above objects and other related objects, present invention provide the technical scheme that a kind of multiple physical field couples
Bioreactor, including being sequentially communicated the biological respinse tank body of setting, ultrasonic wave generates and control device, illumination generate and control
Device and magnetic field generation and control device, the magnetic field, which is generated, is connected to setting with control device with the biological respinse tank body；Institute
It states biological reactor and is provided with circulating pump in vitro；The ultrasonic wave generate with control device include supersonic generator and with institute
The container of biological respinse tank body connection is stated, the probe of the supersonic generator is arranged in the container, the container peripheral hardware
It is equipped with the first cooling layer, puigging is provided with outside first cooling layer；The illumination generation and control device include with it is described
First glass tube of reservoir, first glass tube are provided with shading sleeve outside, it is cooling that second are provided with outside the shading sleeve
Layer, the inner wall of the shading sleeve are provided with illuminator, and the illuminator is connect with light control power supply；The magnetic field generates and control
Device processed includes the second glass tube being connected to first glass tube, is provided with electromagnetic coil outside second glass tube, institute
It states and is provided with antimagnetic set outside electromagnetic coil, the electromagnetic coil is connect with pulse or steady magnetic field control power supply.
Preferred technical solution are as follows: first glass tube and second glass tube are helix tube, first glass
Glass pipe is transparent glass pipe.
Preferred technical solution are as follows: the material of the container is metal.
Preferred technical solution are as follows: be provided with chilled(cooling) water return (CWR) in first cooling layer and the second cooling layer.
Preferred technical solution are as follows: the illuminator is LED light.
Preferred technical solution are as follows: the biological respinse tank body, ultrasonic wave, which are generated, to be generated and control with control device, illumination
Device and magnetic field generate and are connected to setting by the way that valve clip is detachable between control device.
Preferred technical solution are as follows: the biological respinse tank body, ultrasonic wave, which are generated, to be generated and control with control device, illumination
Device and magnetic field, which are generated, is provided with circulating pump and flowmeter between control device.
Since above-mentioned technical proposal is used, the present invention has the advantage, that compared with prior art
The bio-reaction system that the present invention designs can sufficiently cultivate different microorganisms under conditions of low cost, to being
Individually sterilizing can be achieved in the cavity of each device and pipeline in system, and maintenance upgrade is easy, can also connect with any type tank body, needle
To microorganism difference culture demand, use can be freely combined, it is applied widely.
Detailed description of the invention
Fig. 1 is overall schematic of the present invention.
Fig. 2 is the portion A enlarged drawing.
Fig. 3 is the portion B enlarged drawing.
Fig. 4 is the portion C enlarged drawing.
In the figures above, biological respinse tank body 1, ultrasonic wave generates and control device 2, supersonic generator 21, container 22,
First cooling layer 23, puigging 24, illumination generation and control device 3, the first glass tube 31, shading sleeve 32, illuminator 33, second
Cooling layer 34, magnetic field generate and control device 4, the second glass tube 41, electromagnetic coil 42, anti-magnetosheath 43, flowmeter 5, valve card
Hoop 6.
Specific embodiment
Embodiments of the present invention are illustrated by particular specific embodiment below, those skilled in the art can be by this explanation
Content disclosed by book is understood other advantages and efficacy of the present invention easily.
Please refer to Fig. 1 ~ Fig. 4.It should be clear that this specification structure depicted in this specification institute accompanying drawings, ratio, size etc., only to
Cooperate the revealed content of specification, so that those skilled in the art understands and reads, being not intended to limit the invention can be real
The qualifications applied, therefore do not have technical essential meaning, the tune of the modification of any structure, the change of proportionate relationship or size
It is whole, in the case where not influencing the effect of present invention can be generated and the purpose that can reach, it should all still fall in disclosed skill
Art content obtains in the range of capable of covering.Meanwhile in this specification it is cited as "upper", "lower", "left", "right", " centre " and
The term of " one " etc. is merely convenient to being illustrated for narration, rather than to limit the scope of the invention, relativeness
It is altered or modified, under the content of no substantial changes in technology, when being also considered as the enforceable scope of the present invention.
Embodiment: as shown in Fig. 1 ~ Fig. 4, a kind of multiple physical field coupling bioreactor, the life including being sequentially communicated setting
Object reacting tank body 1, ultrasonic wave are generated to be generated and control device 4, magnetic with control device 2, illumination generation and control device 3 and magnetic field
Field, which is generated, is connected to setting with control device 4 with biological respinse tank body 1；Circulating pump, biological respinse are provided with outside biological respinse tank body 1
Tank body 1 can carry out deep drainpipe to different liquids, and circulating pump can push fermentation liquid outer circulation, guarantee sufficiently culture；Ultrasonic wave produces
The raw container 22 for including supersonic generator 21 with control device 2 and being connected to biological respinse tank body 1, supersonic generator 21
Probe be arranged in container 22, the first cooling layer 23 is provided with outside container 22, is provided with puigging 24 outside the first cooling layer 23；
Illumination generation includes the first glass tube 31 being connected to container 22 with control device 3, is provided with shading sleeve outside the first glass tube 31
32, shading sleeve 32 is provided with the second cooling layer 34 outside, and the inner wall of shading sleeve 32 is provided with illuminator 33, illuminator 33 and illumination control
Power supply connection processed；It includes the second glass tube 41 being connected to the first glass tube 31, the second glass tube that magnetic field, which is generated with control device 4,
It is provided with electromagnetic coil 42 outside 41, antimagnetic set 43, electromagnetic coil 42 and pulse or steady magnetic field control are provided with outside electromagnetic coil 42
Power supply connection processed.
Firstly, regulate the power of ultrasonic wave on demand, by light control power supply regulate the light intensity of illuminator 33 with
Wavelength controls power supply by pulse or steady magnetic field and regulates alterating and direct current and size of current, it is made to generate stable state or alternation magnetic
；It enables liquid flow to container 22 from biological respinse tank body 1, cool down and flow to the first glass tube 31 after receiving ultrasonic wave, receive light
It flow to the second glass tube 41 according to after, flows back into biological respinse tank body 1 after receiving magnetic field.
Note: ultrasonic wave generates the ultrasonic wave that 20 ~ 25 kHz of frequency, power 0-1800W can be generated with control device 2.Light
The light source of 0 ~ 100,000 lx, different wave length can be generated with control device 3 according to generation.Magnetic field generates and can generate with control device 4
0 ~ 600 mT steady magnetic field of adjustable pulse magnetic field or intensity of 0 ~ 500 mT of 0 ~ 2 kHz of frequency, duty ratio 10% ~ 90% and intensity.
Preferred embodiment are as follows: as shown in Figure 3 and Figure 4, the first glass tube 31 and the second glass tube 41 are helix tube,
First glass tube 31 is transparent glass pipe.So that fermentation liquid receives illumination and the area in magnetic field is abundant, guarantee that microorganism is sufficiently trained
It supports.
Preferred embodiment are as follows: the material of container 22 is metal.The heat transmitting of metal material is relatively fast, can accelerate to hold
The cooling of fermentation liquid in device 22.
Preferred embodiment are as follows: as shown in Figures 2 and 3, be provided in the first cooling layer 23 and the second cooling layer 34
Chilled(cooling) water return (CWR).That is anti-freeze cooling liquid, good cooling results can also be recycled, reduce cost.
Preferred embodiment are as follows: as shown in figure 3, illuminator 33 is LED light.LED light is small in size, light-weight, is convenient for
Installation in shading sleeve 32, and power consumption is low, can reduce cost.
Preferred embodiment are as follows: produced as shown in Figure 1, biological respinse tank body 1, ultrasonic wave are generated with control device 2, illumination
It is raw to be generated with control device 3 and magnetic field and be connected to setting by the way that valve clip 6 is detachable between control device 4.It can pass through
Valve clip 6 connects fastly, and individually sterilizing can be achieved in each cavity and pipeline, and maintenance upgrade is easy, can connect with any type tank body,
For microorganism difference culture demand, use can be freely combined, expand the scope of application.
Preferred embodiment are as follows: produced as shown in Figure 1, biological respinse tank body 1, ultrasonic wave are generated with control device 2, illumination
Raw and control device 3 and magnetic field, which are generated, is provided with circulating pump and flowmeter 5 between control device 4.
The bio-reaction system that the present invention designs can sufficiently cultivate different microorganisms under conditions of low cost,
Individually sterilizing can be achieved in cavity and pipeline to device each in system, and maintenance upgrade is easy, can also connect with any type tank body
It connects, for microorganism difference culture demand, use can be freely combined, it is applied widely.
The above-described embodiments merely illustrate the principles and effects of the present invention, and is not intended to limit the present invention.It is any ripe
The personage for knowing this technology all without departing from the spirit and scope of the present invention, carries out modifications and changes to above-described embodiment.Cause
This, institute is complete without departing from the spirit and technical ideas disclosed in the present invention by those of ordinary skill in the art such as
At all equivalent modifications or change, should be covered by the claims of the present invention.
Claims (7)
1. a kind of multiple physical field coupling bioreactor, it is characterised in that: including being sequentially communicated the biological respinse tank body of setting, surpassing
Sound wave generation is generated and is controlled with control device, illumination generation and control device and magnetic field generation and control device, the magnetic field
Device is connected to setting with the biological respinse tank body；The biological reactor is provided with circulating pump in vitro；The ultrasonic wave generates
The container for including supersonic generator with control device and being connected to the biological respinse tank body, the supersonic generator
Probe setting in the container, is provided with the first cooling layer, puigging is provided with outside first cooling layer outside the container；
The illumination generation and control device include the first glass tube with the reservoir, and screening is provided with outside first glass tube
Light set, the shading sleeve are provided with the second cooling layer outside, and the inner wall of the shading sleeve is provided with illuminator, the illuminator and light
According to control power supply connection；It includes the second glass tube being connected to first glass tube, institute that the magnetic field, which is generated with control device,
State and be provided with electromagnetic coil outside the second glass tube, be provided with outside the electromagnetic coil antimagnetic set, the electromagnetic coil and pulse or
Steady magnetic field controls power supply connection.
2. a kind of multiple physical field coupling bioreactor according to claim 1, it is characterised in that: first glass tube
It is helix tube with second glass tube, first glass tube is transparent glass pipe.
3. a kind of multiple physical field coupling bioreactor according to claim 2, it is characterised in that: the material of the container
For metal.
4. a kind of multiple physical field coupling bioreactor according to claim 3, it is characterised in that: first cooling layer
Chilled(cooling) water return (CWR) is provided with in the second cooling layer.
5. a kind of multiple physical field coupling bioreactor according to claim 4, it is characterised in that: the illuminator is
LED light.
6. a kind of multiple physical field coupling bioreactor according to claim 5, it is characterised in that: the biological reactor
Body, ultrasonic wave, which are generated, passes through valve between control device, illumination generation and control device and magnetic field generation and control device
The detachable connection setting of clip.
7. a kind of multiple physical field coupling bioreactor according to claim 6, it is characterised in that: the biological reactor
Body, ultrasonic wave are generated to be provided between control device, illumination generation and control device and magnetic field generation and control device and be followed
Ring pump and flowmeter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910881952.1A CN110437993A (en) | 2019-09-18 | 2019-09-18 | A kind of multiple physical field coupling bioreactor |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910881952.1A CN110437993A (en) | 2019-09-18 | 2019-09-18 | A kind of multiple physical field coupling bioreactor |
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| Publication Number | Publication Date |
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| CN110437993A true CN110437993A (en) | 2019-11-12 |
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| CN201910881952.1A Pending CN110437993A (en) | 2019-09-18 | 2019-09-18 | A kind of multiple physical field coupling bioreactor |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102618440A (en) * | 2011-01-31 | 2012-08-01 | 中国科学院过程工程研究所 | Ultrasound-enhanced bioreactor |
| CN102994385A (en) * | 2012-12-12 | 2013-03-27 | 中国科学院华南植物园 | Airlift optical, acoustic and magnetic control bioreactor |
| CN109266551A (en) * | 2018-11-17 | 2019-01-25 | 华中农业大学 | A kind of novel optomagnetic strap lift-type bioreactor |
| CN210974690U (en) * | 2019-09-18 | 2020-07-10 | 中国科学院合肥物质科学研究院 | Multi-physical-field coupling bioreactor |
-
2019
- 2019-09-18 CN CN201910881952.1A patent/CN110437993A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102618440A (en) * | 2011-01-31 | 2012-08-01 | 中国科学院过程工程研究所 | Ultrasound-enhanced bioreactor |
| CN102994385A (en) * | 2012-12-12 | 2013-03-27 | 中国科学院华南植物园 | Airlift optical, acoustic and magnetic control bioreactor |
| CN109266551A (en) * | 2018-11-17 | 2019-01-25 | 华中农业大学 | A kind of novel optomagnetic strap lift-type bioreactor |
| CN210974690U (en) * | 2019-09-18 | 2020-07-10 | 中国科学院合肥物质科学研究院 | Multi-physical-field coupling bioreactor |
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