CN110433086A - A kind of blue silk glue protein freeze-drying ball and preparation method thereof - Google Patents
A kind of blue silk glue protein freeze-drying ball and preparation method thereof Download PDFInfo
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- CN110433086A CN110433086A CN201910839062.4A CN201910839062A CN110433086A CN 110433086 A CN110433086 A CN 110433086A CN 201910839062 A CN201910839062 A CN 201910839062A CN 110433086 A CN110433086 A CN 110433086A
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- Prior art keywords
- freeze
- glue protein
- drying
- silk glue
- liquid
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/0241—Containing particulates characterized by their shape and/or structure
- A61K8/025—Explicitly spheroidal or spherical shape
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Dermatology (AREA)
- Emergency Medicine (AREA)
- Cosmetics (AREA)
- Medicinal Preparation (AREA)
Abstract
A kind of blue silk glue protein freeze-drying ball, it by mass percentage include following components: silk gum protein 10 %-30%, Sodium Hyaluronate (ten thousand dalton of 100-140) 0.1%-10%, mannitol 25%-35%, pulullan polysaccharide 10%-20%, -1 copper 0.2%-1.5% of tripeptides, aseptic deionized water add to 100%.(1) Sodium Hyaluronate, mannitol, pulullan polysaccharide are weighed and adds aseptic deionized water, is heated to 70-80 DEG C, is configured to homogeneous transparent with liquid；(2) when being cooled to 25-35 DEG C when match liquid in step 1, silk glue protein, -1 copper of tripeptides is added, stirs evenly；(3) will with liquid carry out it is sterile freeze, plastotype balling-up；(4) ice hockey is transferred in sterilizing pre-freeze extremely -40--50 DEG C of freeze dryers in advance.Solve poor existing freeze-drying ball forming effect, easy atrophy, it is easily broken, color is single the problems such as.
Description
Technical field
The present invention relates to cosmetics, especially a kind of blue silk glue protein freeze-drying ball and preparation method thereof.
Background technique
That there are mouldings is poor for preceding commercially available freeze-drying ball, is easy that atrophy is broken, phenomenon not easy to maintain.Present invention application silk gum egg
It is white that porous easy rehydration dissolution, stable structure are capable of forming after freeze-drying as main freeze-drying frame, while adding sweet dew
Sugar alcohol, pulullan polysaccharide and Sodium Hyaluronate play the role of firm form, are unlikely to deform freeze-drying ball in transport preservation process.
Summary of the invention
Technical problem to be solved by the invention is to provide a kind of blue silk glue protein freeze-drying ball and preparation method thereof, solutions
Certainly existing freeze-drying ball forming effect is poor, easy atrophy, it is easily broken, color is single the problems such as.
In order to solve the above technical problems, the technical scheme is that ball is lyophilized in a kind of blue silk glue protein, by quality
Percentage includes following components: silk gum protein 10 %-30%, Sodium Hyaluronate (ten thousand dalton of 100-140) 0.1%-10%,
Mannitol 25%-35%, pulullan polysaccharide 10%-20%, -1 copper 0.2%-1.5% of tripeptides, aseptic deionized water add to
100%.Spherical complete smooth, azure bright and clean, the non-breakable, atrophy of color of ball is lyophilized in the present invention.Collocation solvent or pure water make
With, skin sense is salubrious, soft and smooth, be easy absorb, do not stick it is greasy, have repair moisture-keeping efficacy.
Preparation method of the present invention the following steps are included:
(1) Sodium Hyaluronate, mannitol, pulullan polysaccharide are weighed and adds aseptic deionized water, 70-80 DEG C is heated to, matches
Homogeneous transparent is made with liquid；
(2) when being cooled to 25-35 DEG C when match liquid in step (1), silk glue protein, -1 copper of tripeptides is added, stirs evenly；
(3) will obtained by step (2) with liquid carried out under GMP production environment it is sterile freeze, plastotype is that 1cm is spherical at diameter；
(4) gained ice hockey in step (3) sterilizing pre-freeze in advance is transferred to directly to carry out into -40--50 DEG C of freeze dryers
It is lyophilized within 20-40 hours；
(5) freeze-drying is completed, and in temperature is 20-30 DEG C after detection is qualified, and humidity is in 45% or less environment, bottled,
Sealing.
The beneficial effect of the invention compared with the existing technology is:
1, silk glue protein: extracting from natural silk, contains 18 kinds of amino acid, can help to repair impaired skin group
It knits；Silk gum protein hydrolyzate penetration is extremely strong and is able to suppress tyrosinase generation melanin, has white-skinned face function.In addition,
Its fibre structure facilitates freeze-drying ball forming and stablizes；
2, Sodium Hyaluronate: macromolecular structure helps to enhance water lock moisture retention, and reticular structure can when with liquid freeze-drying
Solute transfer is reduced, makes that uniform in color after ball is lyophilized is lyophilized, and firm；Salubrious soft and smooth skin sense can be brought, use feeling is enhanced；
3, mannitol, pulullan polysaccharide；Collocation used as main excipients, can effective protection silk glue protein and
- 1 copper activity of tripeptides increases freeze-drying ball color, forms freeze-drying skeleton, makes that the non-breakable atrophy of ball is lyophilized；
4, -1 copper of tripeptides: being commonly called as blue copper peptide, has effects that repair；Natural blue color is able to solve freeze-drying ball single white
The phenomenon that dull coloring.
Specific embodiment
Embodiment 1
A kind of blue silk glue protein freeze-drying ball, is formulated as follows: silk gum protein 10 %, hyalomitome by mass percentage
Sour sodium (1,000,000 dalton) 2%, mannitol 25%, pulullan polysaccharide 10%, -1 copper 0.3% of tripeptides, aseptic deionized water adds
To 100%.
Preparation method of the present invention the following steps are included:
(1) Sodium Hyaluronate, mannitol, pulullan polysaccharide are weighed and adds aseptic deionized water, 70-80 DEG C is heated to, matches
Homogeneous transparent is made with liquid；
(2) when being cooled to 25-35 DEG C when match liquid in step 1, silk glue protein, -1 copper of tripeptides is added, stirs evenly；
(3) will obtained by step 2 with liquid carried out under GMP production environment it is sterile freeze, plastotype balling-up (diameter 1cm)；
(4) gained ice hockey in step 3 is transferred to sterilizing pre-freeze in advance and directly carries out 20- into -40--50 DEG C of freeze dryers
It is lyophilized within 40 hours；
(5) freeze-drying is completed, and in temperature is 20-30 DEG C after detection is qualified, and humidity is in 45% or less environment, bottled,
Sealing.
Embodiment 2
A kind of blue silk glue protein freeze-drying ball, is formulated as follows: silk gum protein 25 %, hyalomitome by mass percentage
Sour sodium (1,100,000 dalton) 2%, mannitol 25%, pulullan polysaccharide 10%, -1 copper 0.3% of tripeptides, aseptic deionized water adds
To 100%.
Preparation method of the present invention the following steps are included:
(1) Sodium Hyaluronate, mannitol, pulullan polysaccharide are weighed and adds aseptic deionized water, 70-80 DEG C is heated to, matches
Homogeneous transparent is made with liquid；
(2) when being cooled to 25-35 DEG C when match liquid in step 1, silk glue protein, -1 copper of tripeptides is added, stirs evenly；
(3) will obtained by step 2 with liquid carried out under GMP production environment it is sterile freeze, plastotype balling-up (diameter 1cm)；
(4) gained ice hockey in step 3 is transferred to sterilizing pre-freeze in advance and directly carries out 20- into -40--50 DEG C of freeze dryers
It is lyophilized within 40 hours；
(5) freeze-drying is completed, and in temperature is 20-30 DEG C after detection is qualified, and humidity is in 45% or less environment, bottled,
Sealing.
Embodiment 3
A kind of blue silk glue protein freeze-drying ball, is formulated as follows: silk gum protein 25 %, hyalomitome by mass percentage
Sour sodium (1,200,000 dalton) 2%, mannitol 25%, pulullan polysaccharide 10%, -1 copper 0.6% of tripeptides, aseptic deionized water adds
To 100%.
Preparation method of the present invention the following steps are included:
(1) Sodium Hyaluronate, mannitol, pulullan polysaccharide are weighed and adds aseptic deionized water, 70-80 DEG C is heated to, matches
Homogeneous transparent is made with liquid；
(2) when being cooled to 25-35 DEG C when match liquid in step 1, silk glue protein, -1 copper of tripeptides is added, stirs evenly；
(3) will obtained by step 2 with liquid carried out under GMP production environment it is sterile freeze, plastotype balling-up (diameter 1cm)；
(4) gained ice hockey in step 3 is transferred to sterilizing pre-freeze in advance and directly carries out 20- into -40--50 DEG C of freeze dryers
It is lyophilized within 40 hours；
(5) freeze-drying is completed, and in temperature is 20-30 DEG C after detection is qualified, and humidity is in 45% or less environment, bottled,
Sealing.
Embodiment 4
A kind of blue silk glue protein freeze-drying ball, is formulated as follows: silk gum protein 10 %, hyalomitome by mass percentage
Sour sodium (1,300,000 dalton) 2%, mannitol 25%, pulullan polysaccharide 10%, -1 copper 0.6% of tripeptides, aseptic deionized water adds
To 100%.
Preparation method of the present invention the following steps are included:
(1) Sodium Hyaluronate, mannitol, pulullan polysaccharide are weighed and adds aseptic deionized water, 70-80 DEG C is heated to, matches
Homogeneous transparent is made with liquid；
(2) when being cooled to 25-35 DEG C when match liquid in step 1, silk glue protein, -1 copper of tripeptides is added, stirs evenly；
(3) will obtained by step 2 with liquid carried out under GMP production environment it is sterile freeze, plastotype balling-up (diameter 1cm)；
(4) gained ice hockey in step 3 is transferred to sterilizing pre-freeze in advance and directly carries out 20- into -40--50 DEG C of freeze dryers
It is lyophilized within 40 hours；
(5) freeze-drying is completed, and in temperature is 20-30 DEG C after detection is qualified, and humidity is in 45% or less environment, bottled,
Sealing.
Embodiment 5
A kind of blue silk glue protein freeze-drying ball, is formulated as follows: silk gum protein 10 %, hyalomitome by mass percentage
Sour sodium (ten thousand dalton of 100-140) 5%, mannitol 25%, pulullan polysaccharide 10%, -1 copper 0.6% of tripeptides, sterile deionization
Water adds to 100%.
Preparation method of the present invention the following steps are included:
(1) Sodium Hyaluronate, mannitol, pulullan polysaccharide are weighed and adds aseptic deionized water, 70-80 DEG C is heated to, matches
Homogeneous transparent is made with liquid；
(2) when being cooled to 25-35 DEG C when match liquid in step 1, silk glue protein, -1 copper of tripeptides is added, stirs evenly；
(3) will obtained by step 2 with liquid carried out under GMP production environment it is sterile freeze, plastotype balling-up (diameter 1cm)；
(4) gained ice hockey in step 3 is transferred to sterilizing pre-freeze in advance and directly carries out 20- into -40--50 DEG C of freeze dryers
It is lyophilized within 40 hours；
(5) freeze-drying is completed, and in temperature is 20-30 DEG C after detection is qualified, and humidity is in 45% or less environment, bottled,
Sealing.
The test of subject's white-skinned face function: 60 subjects are selected at random, are divided into 6 groups, every group of 10 people, in arm same position
One piece of region " 4cm*4cm " delimited, freeze-drying ball prepared by 1-5 of the embodiment of the present invention and commercially available a whitening are declared into freeze-drying ball point
Not Yong 2-3ml dissolved in purified water, every group of people correspond to a kind of freeze-drying ball and carry out uniformly being applied to defined area respectively using test,
Once a day.With dermal melanin tester before smearing, the 1st week, the 2nd week, the 3rd week, the 4th week test defined area melanin
Content, average result record are as follows:
Conclusion: invention freeze-drying ball has obvious white-skinned face function, and wherein silk glue protein and blue copper peptide content get over high effect
It is more obvious, the two has synergistic function, can effectively reduce dermal melanin content.
Claims (2)
1. ball is lyophilized in a kind of blue silk glue protein, it is characterised in that: by mass percentage include following components: silk glue protein
10%-30%, Sodium Hyaluronate (ten thousand dalton of 100-140) 0.1%-10%, mannitol 25%-35%, pulullan polysaccharide
10%-20%, -1 copper 0.2%-1.5% of tripeptides, aseptic deionized water add to 100%.
2. a kind of preparation method of blue silk glue protein freeze-drying ball as described in claim 1, which is characterized in that including following step
It is rapid:
(1) Sodium Hyaluronate, mannitol, pulullan polysaccharide are weighed and adds aseptic deionized water, 70-80 DEG C is heated to, is configured to
Homogeneous transparent matches liquid；
(2) when being cooled to 25-35 DEG C when match liquid in step (1), silk glue protein, -1 copper of tripeptides is added, stirs evenly；
(3) will obtained by step (2) with liquid carried out under GMP production environment it is sterile freeze, plastotype is that 1cm is spherical at diameter；
(4) gained ice hockey in step (3) is transferred in sterilizing pre-freeze extremely -40--50 DEG C of freeze dryers in advance, directly progress 20-40
Hour freeze-drying；
(5) freeze-drying is completed, and in temperature is 20-30 DEG C after detection is qualified, humidity is to be bottled, sealed in 45% or less environment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910839062.4A CN110433086A (en) | 2019-09-05 | 2019-09-05 | A kind of blue silk glue protein freeze-drying ball and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910839062.4A CN110433086A (en) | 2019-09-05 | 2019-09-05 | A kind of blue silk glue protein freeze-drying ball and preparation method thereof |
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| CN110433086A true CN110433086A (en) | 2019-11-12 |
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| CN201910839062.4A Pending CN110433086A (en) | 2019-09-05 | 2019-09-05 | A kind of blue silk glue protein freeze-drying ball and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112891230A (en) * | 2021-03-02 | 2021-06-04 | 广州栋方生物科技股份有限公司 | Freeze-dried ball and preparation method thereof |
| CN113502322A (en) * | 2021-06-15 | 2021-10-15 | 苏州天隆生物科技有限公司 | PCR freeze-drying reagent and preparation method thereof |
| CN115177547A (en) * | 2022-07-20 | 2022-10-14 | 天津瑞湃尔生物科技有限公司 | MiRNA freeze-drying protective agent with repairing function and preparation method of freeze-drying balls of miRNA freeze-drying protective agent |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105078783A (en) * | 2015-09-06 | 2015-11-25 | 无锡贝迪生物工程有限公司 | Instant collagen lyophilized ball and preparation method thereof |
| CN109350555A (en) * | 2018-12-08 | 2019-02-19 | 江苏江山聚源生物技术有限公司 | A kind of reparation skin recombination human source collagen freeze-drying ball and preparation method thereof |
| CN109512691A (en) * | 2018-11-21 | 2019-03-26 | 广州丽彦妆生物科技有限公司 | A kind of indigo plant copper peptide freeze-dried powder preparation and preparation method thereof |
| CN110123666A (en) * | 2019-05-30 | 2019-08-16 | 王晋祥 | A kind of multiple-effect maintenance freeze-drying film |
-
2019
- 2019-09-05 CN CN201910839062.4A patent/CN110433086A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105078783A (en) * | 2015-09-06 | 2015-11-25 | 无锡贝迪生物工程有限公司 | Instant collagen lyophilized ball and preparation method thereof |
| CN109512691A (en) * | 2018-11-21 | 2019-03-26 | 广州丽彦妆生物科技有限公司 | A kind of indigo plant copper peptide freeze-dried powder preparation and preparation method thereof |
| CN109350555A (en) * | 2018-12-08 | 2019-02-19 | 江苏江山聚源生物技术有限公司 | A kind of reparation skin recombination human source collagen freeze-drying ball and preparation method thereof |
| CN110123666A (en) * | 2019-05-30 | 2019-08-16 | 王晋祥 | A kind of multiple-effect maintenance freeze-drying film |
Non-Patent Citations (1)
| Title |
|---|
| 广州美颜魔法生物科技有限公司: "缔湶医美多肽蛋白冻干丝+多肽蓝铜溶酶", 《HTTP://FTBA.NMPA.GOV.CN:8181/FTBAN/ITOWNET/HZP_BA/FW/PZ.JSP?PROCESSID=20180509211756T8WAK&NID=20180509211756T8WAK》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112891230A (en) * | 2021-03-02 | 2021-06-04 | 广州栋方生物科技股份有限公司 | Freeze-dried ball and preparation method thereof |
| CN113502322A (en) * | 2021-06-15 | 2021-10-15 | 苏州天隆生物科技有限公司 | PCR freeze-drying reagent and preparation method thereof |
| CN115177547A (en) * | 2022-07-20 | 2022-10-14 | 天津瑞湃尔生物科技有限公司 | MiRNA freeze-drying protective agent with repairing function and preparation method of freeze-drying balls of miRNA freeze-drying protective agent |
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Application publication date: 20191112 |