CN110423750A - Cotton fiber length correlation microRNA396 and its precursor dna and application - Google Patents
Cotton fiber length correlation microRNA396 and its precursor dna and application Download PDFInfo
- Publication number
- CN110423750A CN110423750A CN201910646071.1A CN201910646071A CN110423750A CN 110423750 A CN110423750 A CN 110423750A CN 201910646071 A CN201910646071 A CN 201910646071A CN 110423750 A CN110423750 A CN 110423750A
- Authority
- CN
- China
- Prior art keywords
- fiber length
- cotton fiber
- dna
- cotton
- precursor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering N.A.
- C12N2310/141—MicroRNAs, miRNAs
Abstract
The invention discloses a kind of cotton fiber length correlation microRNA and encode the precursor dna of cotton fiber length correlation microRNA, the cotton fiber length correlation microRNA sequence is the nucleic acid sequence such as SEQ ID NO:1 shown in or the sequence as shown in SEQ ID NO:1 pass through one or several nucleic acid substitution and/or deletion and/or addition and it is relevant to cotton fiber length derived from nucleic acid sequence.Cotton fiber length correlation microRNA and its precursor dna provided by the invention, import cotton for precursor dna, can significantly improve cotton fiber length.The present invention has great importance for cultivating new cotton variety, is suitable for promoting and applying.
Description
Technical field
The present invention relates to cotton fiber length correlation microRNA and its precursor dna and applications, belong to field of biotechnology.
Background technique
China is that sown areas of cotton are maximum in the world, the most country of cotton fiber consumption figure.Cotton fiber is also one
Model (the Chen et al. 2007b of a good research single cells grown development; Wendel et al. 2003).The world
The cotton of upper 95 % or more be generated by upland cotton, this is because it have extensive adaptability and higher yield, and its
Remaining 5 % output of cotton mainly from sea island cotton, the fiber of sea island cotton has the length, intensity and thinner mark of superelevation grand
Value.Cotton fiber be differentiated from the epidermis of cottonseed come it is unicellular, every cottonseed, which all contains about 25000 cells, to be sent out
It is bred as fibrocyte (Kim et al. 2001; Lee et al. 2007).Cotton fiber length some can reach 3 ~ 5 cm.Cotton
The main ingredient of fiber is cellulose, and content can achieve 95 % or more.These characteristics of cotton fiber cell make cotton fiber
As research cell differentiation and cell rapid elongation and research cellulose biosynthesis and the good model utilized.It is long
Fiber height is the main target of cotton breeding than the cotton fiber of strong low mic value.
The Research progress on Function of miRNA is slow during cotton fiber development at present, although a series of research has been sent out
Effect of the existing miRNAs in adjusting Fibre Development, but definite function of few researchs to some miNRAs in cotton fiber
It can be carried out research.
Summary of the invention
In view of this, specifically being adopted the present invention provides cotton fiber length correlation microRNA and its precursor dna and application
With following technical solution:
Cotton fiber length correlation microRNA is obtained from cotton, is named as MIR396, and sequence is as shown in SEQ ID NO: 1
Nucleic acid sequence；
Or, the sequence as shown in SEQ ID NO: 1 is by the substitution and/or deletion and/or addition of one or several nucleic acid and and cotton
Nucleic acid sequence derived from flower fibre length is relevant.
The nucleic acid sequence of above-mentioned microRNA can be artificial synthesized, can also directly clone to obtain in cotton, such as by by SEQ
One or several nucleic acid are lacked in nucleic acid sequence shown in ID NO: 1, and/or carry out being mutated for one or several base-pairs
It arrives.
The present invention also provides the precursor dna for encoding above-mentioned cotton fiber length correlation microRNA, gene order is
Any one DNA molecular below:
(1) code area DNA molecular as shown in SEQ ID NO: 2；
(2) hybridize and encode the DNA molecular of microRNA related with cotton fiber length to DNA molecular in (1)；
(3) there is the DNA of 90% or more homology and coding and cotton fiber length GAP-associated protein GAP with DNA molecular in (1) or (2)
Molecule.
Further, the condition that (2) hybridize with DNA molecular shown in SEQ ID NO: 2 is 0.1 × SSPE (or 0.1
× SSC), the solution of 0.1%SDS hybridizes at 65 DEG C in DNA RNA hybrid experiment and washes film.
The present invention also provides above-mentioned precursor dnas in preparation and reorganization expression vector, expression cassette, transgenic cell line or recombination
Application in bacterium.
The recombinant expression of cotton fiber length correlation microRNA sequence can be contained with existing plant expression vector construction
Carrier.Plant expression vector includes double base agrobacterium vector and the carrier etc. that can be used for plant micropellet bombardment.Use cotton fiber
When length correlation microRNA constructs recombinant expression carrier, can before its transcription initiation nucleotide plus it is any it is enhanced,
Composing type, organizing specific type or inducible promoter, they can be used alone or are used in combination with other plant promoters；This
Outside, when constructing recombinant expression carrier using cotton fiber length correlation microRNA, enhancer, including translation enhancing also can be used
Son or transcriptional enhancer.For the ease of transgenic plant cells or plant are identified and screened, plant used can be expressed
Carrier is processed, and the expression in plant, which is such as added, can produce the enzyme of color change or the gene, resistant of luminophor
Antibiotic marker or anti-chemical reagent marker gene etc..
Segment between BamHI the and SacI restriction enzyme site of pBI121 carrier is concretely replaced with sequence by recombinant expression carrier
The sequence 2 of list obtained recombinant expression carrier of DNA molecular shown in 5 ' 1-136, the ends；
Or, recombinant expression carrier concretely will be between SpeI the and AscI restriction enzyme site of pCLCrV-A cotton curve leaf disease poisonous carrier
Segment replaces with the recombinant expression carrier that the sequence 2 of the sequence table DNA molecular shown in 5 ' 1-136, the ends obtains.
The present invention also provides above-mentioned cotton fiber length correlation microRNA, or, above-mentioned precursor dna regulates and controls cotton fiber
Method in length, step are as follows: it is long to obtain different fibers for the expression quantity for changing cotton fiber length correlation microRNA in cotton
The cotton of degree.
In method, cotton fiber length correlation microRNA sequence can import cotton by any of the above-described recombinant expression carrier
Flower.
Recombinant expression carrier can pass through Ti-plasmids, Ri plasmid, plant viral vector, directly delivered DNA, microinjection, electricity
Lead, the conventional biology methods such as mediated by agriculture bacillus are transformed into cotton cells or tissue in.
The present invention provides cotton fiber length correlation microRNA and its precursor dnas, its precursor DNA sequence is imported cotton
Flower increases the expression quantity of cotton fiber length correlation microRNA in cotton, can significantly improve cotton fiber length.The present invention
Have great importance for cultivating new cotton variety, is suitable for promoting and applying.
The present invention also provides above-mentioned cotton fiber length correlation microRNA, or, above-mentioned precursor dna, or, the above method
Application in plant breeding.
Further, plant is Malvaceae cotton, including upland cotton, sea island cotton, Asiatic cotton.
Detailed description of the invention
The qRT-PCR detection of MIR396 in the cotton plants that Fig. 1 infects for 2 cotton curve leaf disease poisonous carrier of the embodiment of the present invention
As a result.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
In the examples below, pCLCrV carrier is document Gu Zhouhang, Huang Changjun, Li Fangfang
et al. A versatile system for functional analysis of genes and microRNAs in
Cotton. pCLCrV carrier involved in [J] .Plant Biotechnol. J., 2014,12:638-49；
The Zhengzhou Agrobacterium GV3101(Shang Yi Biotechnology Co., Ltd, Hua Yue foreign brand)；
Cotton variety: nakamise 24(the Chinese Academy of Agriculture Science and Technologys Cotton Research Institute)；
Other do not do the experimental method of specified otherwise, are common experimental method in the art；The material of specified otherwise is not done,
It is common material in the art, quantitative experiment conducted in following embodiment is respectively provided with three repeated experiments, as a result takes
Average value.
Embodiment 1
The acquisition of MIR396 mature sequence and its precursor sequence miR396a
Small RNA in cotton land-sea backcrossing inbred strais " Long " and 10 days fibers of " Short " Post flowering is extracted, and is carried out
MiRNA high-flux sequence.By the analysis of a large amount of sequences, expression analysis and functional verification, one is had found from sequencing result
The expression quantity and fibre length of miRNA is positively correlated at significant.Its mature sequence as shown in sequence SEQ ID NO: 1 of sequence table,
The precursor sequence of the miRNA can be generated as shown in sequence SEQ ID NO: 2 of sequence table.
Maturation miRNA shown in sequence SEQ ID NO: 1 by sequence table is named as MIR396.The miRNA will be generated
Precursor sequence be named as miR396a.
Embodiment 2
The functional verification of mature MIR396 and its precursor miR396a
One, virus induction is overexpressed the acquisition of plant
1, the segment between SpeI the and AscI restriction enzyme site of pCLCrV-A carrier the building of recombinant expression carrier: is replaced with into sequence
Sequence SEQ ID NO: 2 of table from 5 ' end 1-136 shown in DNA molecular, obtain recombinant expression carrier pCLCrV-A::
MiR396a(sequence verification).
2, the recombinant expression carrier pCLCrV-A::miR396a for obtaining step 1 imports Agrobacterium GV3101, is recombinated
Bacterium GV3101::miR396a.
3, the recombinant bacterium pCLCrV-A::miR396a that step 2 obtains is transferred to receptor cotton variety by cotyledon injection method
In nakamise 24, follow the steps below:
(1) by pCLCrV-A::miR396a Agrobacterium and assist conversion pCLCrV-B Agrobacterium bacterium solution respectively containing rifampin,
Expand numerous in the resistance LB liquid medium of streptomysin and kanamycins, expand numerous condition: 28 DEG C, 190 rpm, culture is general
16h.Expand numerous to bacteria solution active OD600Between 1.5 to 2.0；
(2) 4000 rpm are centrifuged bacterium solution, 10min, are suspended again using conversion medium to the thallus after centrifugation,
Adjust OD6001.5 or so；
(3) bacterium solution after suspending again is protected from light at 25 DEG C stands 3h and the above time；
(4) pCLCrV-B bacterium solution is connected with pCLCrV-A empty carrier, pCLCrV- positive control, pCLCrV-A::miR396a respectively
There is the bacterium solution of purpose gene to be uniformly mixed according to isometric；
(5) cotton seeds are soaked seed, nursery, bacterium solution is carried out when cotyledon will flatten and is infected.With syringe needle in cotton
Beggar's leaf back marks wound, and then the bacterium solution of mixing is injected into cotyledon；
(6) the cotton plants dark processing after injecting is cultivated in 25 DEG C of greenhouse later for 24 hours；
(7) positive control wins young leaflet tablet to the cotton plants of all injections after showing blade albefaction phenotype, is split using TPS
Solution liquid is cracked and is carried out PCR amplification, is identified positive plant and is transplanted seedlings to cotton greenhouse.
The preparation of conversion medium is as shown in table 1；
The preparation of 1 conversion medium of table
| Nomenclature of drug | Working solution concentration |
| MgCl2 | 10 mM |
| MES 2-(4-Morpholino)ethanesulfonic acid | 10 mM |
| AS acetosyringone(DMSO dissolution) | 100 μM |
Two, the acquisition of viral empty carrier plant
Recombinant expression carrier pCLCrV-A::miR396a is substituted using pCLCrV-A empty carrier, is operated according to step 3-4,
It obtains turning viral empty carrier strain.
Three, turn the qRT-PCR detection of viral vectors plant
Plant to be measured: nakamise 24(WT), virus induction is overexpressed the plant of strain (V1-V3), turns a viral empty carrier strain and plant
Strain.
1, the small RNA in 10 days fibers of cotton Post flowering to be measured is extracted.
2, MIR396 reverse transcription is by the RNA obtained using step 1 as template, and using neck ring neck ring primer RT-MIR396
cDNA.Using primer qRT-MIR396-F and primer qRT-MIR396-R composition primer pair carry out quantitative fluorescent PCR, detection to
Survey the expression of maturation miRNA:MIR396 in plant；The primer pair formed using primer Ghubq6-F and primer Ghubq6-R
Detect reference gene UBQ6.
RT-MIR396:
GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGA CAGTTC(5 ' -3 ')；
qRT-MIR396-F:CACAGCUUUCUUGAACUG(5 ' -3 ')；
qRT-MIR396-R:GTGCAGGGTCCGAGGTATTC(5 ' -3 ')；
Ghubq6-F:CATTTCTCGATTTGTGCGTGTC(5 ' -3 ')；
Ghubq6-R:GGGGACATCCGATAAAATTGG(5 ' -3 ').
As a result as shown in Figure 1.The result shows that three viruses are overexpressed in strain V1-V3 relative to nakamise 24(WT)
The all normal overexpression of MIR396.The expression for turning MIR396 in empty carrier strain is identical as nakamise 24(WT).
Four, Function Identification
Plant to be measured: nakamise 24(WT), virus induction is overexpressed the plant of strain (V1-V3), turns a viral empty carrier strain and plant
Strain.
Mature fibers linear measure longimetry
It is used in Anyang province Chinese agriculture rural area subordinate category cotton fiber quality test center and uses high fiber information system
(AFIS) measurement of fibre length, each plant measurement no less than 3 single bells are carried out as unit of single bell.
The results are shown in Table 2.
2 mature fibers length measurment result of table
The result shows that virus induction is overexpressed strain relative to nakamise 24(WT), there are significant differences for mature fibers length.It is empty
Carrier strain mature fibers length and nakamise 24(WT) indifference.
The above results explanation: microRNA:MIR396 of the invention has the function of adjusting mature fibre length.
Sequence table
<110>the Chinese Academy of Agriculture Science and Technologys Cotton Research Institute
<120>cotton fiber length correlation microRNA396 and its precursor dna and application
<141> 2019-07-17
<160> 2
<170> SIPOSequenceListing 1.0
<210> 1
<211> 21
<212> RNA
<213>artificial sequence (Artificial Sequence)
<400> 1
uuccacagcu uucuugaacu g 21
<210> 2
<211> 136
<212> RNA
<213>artificial sequence (Artificial Sequence)
<400> 2
cuucguauuc uuccacagcu uucuugaacu gcaauuucau guaaucagau cauucauuca 60
uucguugucg uugauggugc ugcauauaua cgaguacguu guugcgguuc aauaaagcug 120
ugggaagaua caaaca 136
Claims (7)
1. cotton fiber length correlation microRNA, which is characterized in that the cotton fiber length correlation microRNA sequence is
The nucleic acid sequence as shown in SEQ ID NO: 1；
Or, the sequence as shown in SEQ ID NO: 1 is by the substitution and/or deletion and/or addition of one or several nucleic acid and and cotton
Nucleic acid sequence derived from flower fibre length is relevant.
2. encoding the precursor dna of cotton fiber length correlation microRNA described in claim 1.
3. the precursor of cotton fiber length correlation microRNA described in a kind of coding claim 1 according to claim 2
DNA, which is characterized in that the gene order of the precursor dna is any one DNA molecular below:
(1) code area DNA molecular as shown in SEQ ID NO: 2；
(2) hybridize and encode the DNA molecular of microRNA related with cotton fiber length to DNA molecular in (1)；
(3) there is the DNA of 90% or more homology and coding and cotton fiber length GAP-associated protein GAP with DNA molecular in (1) or (2)
Molecule.
4. the precursor of cotton fiber length correlation microRNA described in a kind of coding claim 1 according to claim 3
DNA, which is characterized in that (2) condition of hybridization described in is 0.1 × SSPE, and the solution of 0.1%SDS is miscellaneous in DNA or RNA
It hands over and hybridizes at 65 DEG C in experiment and wash film；
Or, the solution of 0.1 × SSC, 0.1%SDS, hybridize at 65 DEG C in DNA RNA hybrid experiment and wash film.
5. precursor dna described in claim any one of 2-4 preparation and reorganization expression vector, expression cassette, transgenic cell line or
Application in recombinant bacterium.
6. cotton fiber length correlation microRNA described in claim 1, or, precursor dna described in claim any one of 2-4
Regulate and control the method in cotton fiber length, which is characterized in that step are as follows: change cotton fiber length correlation microRNA in cotton
Expression quantity, obtain the cotton of different fibre lengths.
7. cotton fiber length correlation microRNA described in claim 1, or, precursor described in claim any one of 2-4
DNA, or, application of claim 6 the method in plant breeding.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910646071.1A CN110423750A (en) | 2019-07-17 | 2019-07-17 | Cotton fiber length correlation microRNA396 and its precursor dna and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910646071.1A CN110423750A (en) | 2019-07-17 | 2019-07-17 | Cotton fiber length correlation microRNA396 and its precursor dna and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110423750A true CN110423750A (en) | 2019-11-08 |
Family
ID=68410836
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910646071.1A Pending CN110423750A (en) | 2019-07-17 | 2019-07-17 | Cotton fiber length correlation microRNA396 and its precursor dna and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110423750A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101280304A (en) * | 2008-05-16 | 2008-10-08 | 中国科学院西双版纳热带植物园 | Use of Arabidopsis miR396 micromolecule |
| US20160108411A1 (en) * | 2014-10-14 | 2016-04-21 | Clemson University | Methods and compositions for transgenic plants with enhanced cold tolerance, ability to flower without vernalization requirement and impacted fertility |
| EP1838144B1 (en) * | 2005-01-07 | 2016-08-31 | Oregon State University | Method to trigger rna interference |
| CN106916828A (en) * | 2017-05-03 | 2017-07-04 | 中国林业科学研究院林业研究所 | A kind of growth regulator gene of poplar adjusted and controlled leaf development and its application |
| CN110066823A (en) * | 2018-01-22 | 2019-07-30 | 中国科学院上海生命科学研究院 | MIM396 is increasing Rice Panicle shape and is improving the application in Plant Height of Rice |
-
2019
- 2019-07-17 CN CN201910646071.1A patent/CN110423750A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1838144B1 (en) * | 2005-01-07 | 2016-08-31 | Oregon State University | Method to trigger rna interference |
| CN101280304A (en) * | 2008-05-16 | 2008-10-08 | 中国科学院西双版纳热带植物园 | Use of Arabidopsis miR396 micromolecule |
| US20160108411A1 (en) * | 2014-10-14 | 2016-04-21 | Clemson University | Methods and compositions for transgenic plants with enhanced cold tolerance, ability to flower without vernalization requirement and impacted fertility |
| CN106916828A (en) * | 2017-05-03 | 2017-07-04 | 中国林业科学研究院林业研究所 | A kind of growth regulator gene of poplar adjusted and controlled leaf development and its application |
| CN110066823A (en) * | 2018-01-22 | 2019-07-30 | 中国科学院上海生命科学研究院 | MIM396 is increasing Rice Panicle shape and is improving the application in Plant Height of Rice |
Non-Patent Citations (3)
| Title |
|---|
| 张华: "棉纤维起始发育过程中microRNA的鉴定及功能分析", 《中国博士学位论文全文数据库 农业科技辑》 * |
| 章镇 等: "《果树分子生物学》", 31 October 2012, 上海科学技术出版社 * |
| 金征宇 等: "《基因与纳米探针-医学分子成像理论与实践 中级》", 30 November 2017, 天津科学技术出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| WO2022135246A1 (en) | R gene for controlling matching of soybean-rhizobium, protein and use thereof | |
| CN108623665A (en) | Application of the GhHUB2 albumen in regulation and control cotton fiber length and intensity | |
| CN102757486B (en) | Plant development related protein GA20ox, and encoding gene and application thereof | |
| CN103183732A (en) | Cotton Gh FPP1 protein as well as coding gene and application thereof | |
| CN104558128B (en) | The albumen related to anti-Fusarium graminearum stem rot and its encoding gene and application | |
| CN110343157A (en) | Cotton verticillium wilt related gene GhBONI and its coding albumen and application | |
| CN104611348A (en) | Cotton fiber predominantly-expressed gene, expression vector and applications thereof, and preparation method of transgenic cotton containing gene | |
| CN110066774A (en) | Corn receptoroid kinase gene ZmRLK7 and its application | |
| CN107630020A (en) | Cotton GhTCP4 genes and its application in cotton fiber length is improved | |
| CN108948169B (en) | Protein and gene for promoting synthesis of cotton fiber green pigment, and coding sequence and application thereof | |
| CN110358772A (en) | The OsEBP89 gene and preparation method of raising rice abiotic stress resistance and application | |
| CN110128520A (en) | Moso bamboo PheHDZ45 albumen and its encoding gene and application | |
| CN105566465A (en) | Corn flowering regulatory protein, coding gene and application | |
| CN110205333B (en) | Corn dwarf disease induction gene P3a and construction method and application of genetic transformation system thereof | |
| CN105039345B (en) | The clone of miRNA for enhancing mulberry tree salt resistance ability a kind of and its application | |
| CN108048481A (en) | Application of the RLI1 albumen in adjusting and controlling rice leaf angle | |
| CN104651359B (en) | The bidirectional promoter separated from corn and its application | |
| CN110204600A (en) | BnSPL14 gene, albumen and its application in control cabbage type rape plant type | |
| CN110106269A (en) | Bna-miR156d is in the control developmental application of cabbage type rape branch | |
| CN110423750A (en) | Cotton fiber length correlation microRNA396 and its precursor dna and application | |
| CN109022482A (en) | The method of TRV carrier mediated Gene Silencing systemic infection tree peony seedling | |
| CN110423749A (en) | Cotton fiber length correlation microRNA160 and its precursor dna and application | |
| CN106191104B (en) | Upland cotton transformation event ICR24001 and its specificity identification method | |
| CN110511932A (en) | Cotton fiber length correlation microRNA477 and its precursor dna and application | |
| CN111100868B (en) | Female promotion gene FERR and female inhibition gene FERR-R of populus deltoides and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |