CN110419743A - A kind of oxidation resistant pulvis of natural liver protection and preparation method thereof - Google Patents
A kind of oxidation resistant pulvis of natural liver protection and preparation method thereof Download PDFInfo
- Publication number
- CN110419743A CN110419743A CN201910774448.1A CN201910774448A CN110419743A CN 110419743 A CN110419743 A CN 110419743A CN 201910774448 A CN201910774448 A CN 201910774448A CN 110419743 A CN110419743 A CN 110419743A
- Authority
- CN
- China
- Prior art keywords
- water
- cape jasmine
- pueraria lobata
- water extract
- clam
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000004185 liver Anatomy 0.000 title claims abstract description 49
- 230000003647 oxidation Effects 0.000 title claims abstract description 34
- 238000007254 oxidation reaction Methods 0.000 title claims abstract description 34
- 238000002360 preparation method Methods 0.000 title claims abstract description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 228
- 239000000284 extract Substances 0.000 claims abstract description 192
- 244000111489 Gardenia augusta Species 0.000 claims abstract description 132
- 235000018958 Gardenia augusta Nutrition 0.000 claims abstract description 132
- 244000046146 Pueraria lobata Species 0.000 claims abstract description 129
- 235000010575 Pueraria lobata Nutrition 0.000 claims abstract description 129
- 210000000582 semen Anatomy 0.000 claims abstract description 117
- 235000013372 meat Nutrition 0.000 claims abstract description 74
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 57
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 54
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 54
- 102000004190 Enzymes Human genes 0.000 claims abstract description 27
- 108090000790 Enzymes Proteins 0.000 claims abstract description 27
- 238000005360 mashing Methods 0.000 claims abstract description 21
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 claims abstract description 12
- 238000012545 processing Methods 0.000 claims description 122
- 239000000843 powder Substances 0.000 claims description 117
- 238000004108 freeze drying Methods 0.000 claims description 62
- 239000000203 mixture Substances 0.000 claims description 58
- 238000006243 chemical reaction Methods 0.000 claims description 48
- 238000001976 enzyme digestion Methods 0.000 claims description 48
- 238000007873 sieving Methods 0.000 claims description 43
- 238000010438 heat treatment Methods 0.000 claims description 39
- 108091005804 Peptidases Proteins 0.000 claims description 25
- 239000004365 Protease Substances 0.000 claims description 25
- 235000019419 proteases Nutrition 0.000 claims description 25
- 239000007788 liquid Substances 0.000 claims description 19
- 239000004383 Steviol glycoside Substances 0.000 claims description 18
- 229930182488 steviol glycoside Natural products 0.000 claims description 18
- 235000019411 steviol glycoside Nutrition 0.000 claims description 18
- 150000008144 steviol glycosides Chemical class 0.000 claims description 18
- 235000019202 steviosides Nutrition 0.000 claims description 18
- 238000002156 mixing Methods 0.000 claims description 12
- 210000001835 viscera Anatomy 0.000 claims description 12
- 239000006228 supernatant Substances 0.000 claims description 11
- 235000005979 Citrus limon Nutrition 0.000 claims description 3
- 244000131522 Citrus pyriformis Species 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 3
- 230000003078 antioxidant effect Effects 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 9
- 239000003963 antioxidant agent Substances 0.000 abstract description 7
- 235000006708 antioxidants Nutrition 0.000 abstract description 7
- 238000000034 method Methods 0.000 abstract description 6
- 230000003064 anti-oxidating effect Effects 0.000 abstract description 4
- 238000005119 centrifugation Methods 0.000 abstract description 4
- 230000007071 enzymatic hydrolysis Effects 0.000 abstract description 2
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 abstract description 2
- 230000009849 deactivation Effects 0.000 abstract 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 210
- 235000019441 ethanol Nutrition 0.000 description 136
- 239000000047 product Substances 0.000 description 67
- 150000001875 compounds Chemical class 0.000 description 53
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 30
- 238000011156 evaluation Methods 0.000 description 25
- 102000035195 Peptidases Human genes 0.000 description 22
- 230000000052 comparative effect Effects 0.000 description 19
- 239000000706 filtrate Substances 0.000 description 18
- 238000001914 filtration Methods 0.000 description 18
- 230000001953 sensory effect Effects 0.000 description 18
- 238000002604 ultrasonography Methods 0.000 description 18
- 238000002137 ultrasound extraction Methods 0.000 description 18
- 244000202052 Poncirus trifoliata Species 0.000 description 17
- 235000000404 Poncirus trifoliata Nutrition 0.000 description 17
- 102000004196 processed proteins & peptides Human genes 0.000 description 13
- 108090000145 Bacillolysin Proteins 0.000 description 9
- 102000035092 Neutral proteases Human genes 0.000 description 9
- 108091005507 Neutral proteases Proteins 0.000 description 9
- 235000013399 edible fruits Nutrition 0.000 description 8
- 238000012423 maintenance Methods 0.000 description 8
- 239000000126 substance Substances 0.000 description 8
- 238000010586 diagram Methods 0.000 description 7
- 241000219780 Pueraria Species 0.000 description 6
- 231100000753 hepatic injury Toxicity 0.000 description 6
- 239000003814 drug Substances 0.000 description 5
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 4
- 229930003944 flavone Natural products 0.000 description 4
- 235000011949 flavones Nutrition 0.000 description 4
- 241000411851 herbal medicine Species 0.000 description 4
- -1 oxygen radical Chemical class 0.000 description 4
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 4
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 238000013329 compounding Methods 0.000 description 3
- 229930003935 flavonoid Natural products 0.000 description 3
- 150000002215 flavonoids Chemical class 0.000 description 3
- 235000017173 flavonoids Nutrition 0.000 description 3
- 230000004060 metabolic process Effects 0.000 description 3
- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 2
- 206010067125 Liver injury Diseases 0.000 description 2
- 241000320380 Silybum Species 0.000 description 2
- 235000010841 Silybum marianum Nutrition 0.000 description 2
- 230000001476 alcoholic effect Effects 0.000 description 2
- 230000002075 anti-alcohol Effects 0.000 description 2
- 235000019606 astringent taste Nutrition 0.000 description 2
- 235000019658 bitter taste Nutrition 0.000 description 2
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- 230000003247 decreasing effect Effects 0.000 description 2
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- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
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- 150000002212 flavone derivatives Chemical class 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 230000036542 oxidative stress Effects 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
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- 239000002994 raw material Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
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- 229940083466 soybean lecithin Drugs 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 235000019605 sweet taste sensations Nutrition 0.000 description 2
- 235000019640 taste Nutrition 0.000 description 2
- 208000007082 Alcoholic Fatty Liver Diseases 0.000 description 1
- 208000007848 Alcoholism Diseases 0.000 description 1
- 240000001579 Cirsium arvense Species 0.000 description 1
- 235000005918 Cirsium arvense Nutrition 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 206010016262 Fatty liver alcoholic Diseases 0.000 description 1
- 241001295925 Gegenes Species 0.000 description 1
- 241000123069 Ocyurus chrysurus Species 0.000 description 1
- 229940123973 Oxygen scavenger Drugs 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- 241000241413 Propolis Species 0.000 description 1
- RXUWDKBZZLIASQ-UHFFFAOYSA-N Puerarin Natural products OCC1OC(Oc2c(O)cc(O)c3C(=O)C(=COc23)c4ccc(O)cc4)C(O)C(O)C1O RXUWDKBZZLIASQ-UHFFFAOYSA-N 0.000 description 1
- 241000219100 Rhamnaceae Species 0.000 description 1
- 240000006079 Schisandra chinensis Species 0.000 description 1
- 235000008422 Schisandra chinensis Nutrition 0.000 description 1
- 229930003270 Vitamin B Natural products 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 208000026594 alcoholic fatty liver disease Diseases 0.000 description 1
- 230000003402 anti-promotion Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000036770 blood supply Effects 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
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- 235000009508 confectionery Nutrition 0.000 description 1
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- 230000007123 defense Effects 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 208000010706 fatty liver disease Diseases 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 229930182478 glucoside Natural products 0.000 description 1
- 150000008131 glucosides Chemical class 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 208000006454 hepatitis Diseases 0.000 description 1
- 231100000283 hepatitis Toxicity 0.000 description 1
- 231100000784 hepatotoxin Toxicity 0.000 description 1
- 235000008216 herbs Nutrition 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 231100000518 lethal Toxicity 0.000 description 1
- 230000001665 lethal effect Effects 0.000 description 1
- 230000003859 lipid peroxidation Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 210000005229 liver cell Anatomy 0.000 description 1
- 208000019423 liver disease Diseases 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002398 materia medica Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 210000000214 mouth Anatomy 0.000 description 1
- 150000002829 nitrogen Chemical class 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 230000004792 oxidative damage Effects 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 238000005502 peroxidation Methods 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
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- 230000002265 prevention Effects 0.000 description 1
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- 229940069949 propolis Drugs 0.000 description 1
- 230000006318 protein oxidation Effects 0.000 description 1
- HKEAFJYKMMKDOR-VPRICQMDSA-N puerarin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1C1=C(O)C=CC(C2=O)=C1OC=C2C1=CC=C(O)C=C1 HKEAFJYKMMKDOR-VPRICQMDSA-N 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 239000007901 soft capsule Substances 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 150000003505 terpenes Chemical class 0.000 description 1
- 235000007586 terpenes Nutrition 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 230000007923 virulence factor Effects 0.000 description 1
- 239000000304 virulence factor Substances 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/04—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from fish or other sea animals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/341—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of animal proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/488—Pueraria (kudzu)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/72—Rhamnaceae (Buckthorn family), e.g. buckthorn, chewstick or umbrella-tree
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/74—Rubiaceae (Madder family)
- A61K36/744—Gardenia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/01—Hydrolysed proteins; Derivatives thereof
- A61K38/012—Hydrolysed proteins; Derivatives thereof from animals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/141—Intimate drug-carrier mixtures characterised by the carrier, e.g. ordered mixtures, adsorbates, solid solutions, eutectica, co-dried, co-solubilised, co-kneaded, co-milled, co-ground products, co-precipitates, co-evaporates, co-extrudates, co-melts; Drug nanoparticles with adsorbed surface modifiers
- A61K9/145—Intimate drug-carrier mixtures characterised by the carrier, e.g. ordered mixtures, adsorbates, solid solutions, eutectica, co-dried, co-solubilised, co-kneaded, co-milled, co-ground products, co-precipitates, co-evaporates, co-extrudates, co-melts; Drug nanoparticles with adsorbed surface modifiers with organic compounds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/141—Intimate drug-carrier mixtures characterised by the carrier, e.g. ordered mixtures, adsorbates, solid solutions, eutectica, co-dried, co-solubilised, co-kneaded, co-milled, co-ground products, co-precipitates, co-evaporates, co-extrudates, co-melts; Drug nanoparticles with adsorbed surface modifiers
- A61K9/148—Intimate drug-carrier mixtures characterised by the carrier, e.g. ordered mixtures, adsorbates, solid solutions, eutectica, co-dried, co-solubilised, co-kneaded, co-milled, co-ground products, co-precipitates, co-evaporates, co-extrudates, co-melts; Drug nanoparticles with adsorbed surface modifiers with compounds of unknown constitution, e.g. material from plants or animals
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
Abstract
The invention discloses oxidation resistant pulvis of a kind of natural liver protection and the preparation method and application thereof.This method comprises: clam is taken meat, wash, mashing adds water, is uniformly mixed, obtains mixed liquor；Neutral proteinase is added in mixed liquor, enzymatic hydrolysis, enzyme deactivation, centrifugation obtains clam enzymolysis protein peptide after freeze-dried；Pueraria lobata, hoveniae semoveniae semen and cape jasmine are clayed into power respectively, are sieved, water is then carried out respectively and mentions, obtain corresponding pueraria lobata water extract, hoveniae semoveniae semen water extract and cape jasmine water extract；Then clam enzymolysis protein peptide, pueraria lobata water extract, hoveniae semoveniae semen water extract, cape jasmine water extract are uniformly mixed with corrigent, are sieved, obtain the pulvis.The oxidation resistant pulvis of natural liver protection provided by the invention is to have both liver-protective effect while having anti-oxidation efficacy according to theory of traditional Chinese medical science, especially suitable for anti-oxidant, liver protection crowd in need, the crowd especially often to drink.
Description
Technical field
The invention belongs to Health-care powder technical field more particularly to a kind of oxidation resistant pulvis of natural liver protection and its preparation sides
Method.
Background technique
Liver is one of most important internal organs of body, carries the function of metabolism and bioconversion, and blood supply is rich
Richness, vulnerable to the invasion of the various virulence factors in inside and outside, to cause hepatic injury.The wind that the mankind drink is prevailing for a long time, and liver is
The main place of alcohol metabolism, alcohol can produce a large amount of active oxygens as strong oxidative stress source during organism metabolism,
Lead to liver plasma membrane lipid peroxidation, liver cell DNA oxidative damage etc., long-term excessive consumption of alcohol will lead to alcoholic fatty liver, wine
The liver diseases such as essence hepatitis.Studies have shown that chronic alcoholism lethal number in the whole world accounts for the 5% of all death tolls, about
30% long-term wine-head suffers from ALD, and the death rate of ALD is directly proportional to the intake of alcohol, therefore ALD has seriously threatened the mankind
Life security.A large number of studies show that oxidative stress plays important role in alcoholic liver injury development process, oxidation
It stress refer to body when by various destructive stimulus, internal high activity molecule such as active oxygen radical, active nitrogen class generated
It is more, more than the Scavenging activity of body Antioxidative Defense System, to cause cellular damage, and then cause various diseases.
It is anti-oxidant by anti-lipid peroxidation, protein oxidation and remove free radical, it is adjustable and improve fuselage state,
Physiological function is corrected, to have the function that prevention or treatment disease.Flavone compound causes active oxygen and form nitrogen
Oxidation have inhibiting effect, be good lipid antioxidant and active oxygen scavenger, therefore, Flavonoid substances have good
Antioxidant activity, to alcoholic liver injury have certain protective effect.
Liver protecting product is mainly with Milk Thistle grass, soybean lecithin, vitamin B, propolis etc. for primary raw material at present, abroad
Product occupies biggish market, such as strong field thistle composite sheet for pacifying the protect liver grain, Ai Si alliance (Esmond Natural) of liking (GNC),
Milk Thistle grass complex capsule of Omknic card (Organika) etc., but these product prevailing prices are higher.Domestic protect liver produces
For product mainly using Chinese herbal medicine as primary raw material, China's approved has the health food of assistant protection function to match chemical damage
The highest pueraria lobata of the frequency of occurrences in side, followed by Schisandra chinensis, hoveniae semoveniae semen, cape jasmine etc., main composition is general flavone, thick more
Sugar, Puerarin, it is not significant generally to improve the effect that hepatic injury is by single nutritional ingredient, realizes often through compounding.
Merely using a variety of Chinese herbal medicine extracts as main component, the active constituent of product is relatively single.On this basis, appropriate egg is added
White or polypeptide is conducive to Product Activity diversification, optimizes product effect, as the kudzu root extract soybean lecithin of Schroeder protects
Liver soft capsule.
" the hoveniae semoveniae semen ball " recorded according to " Gegen Decoction ", the Yuan Dynasty's Effective Formulas Tested by Physicians for Generations recorded in " Yan's prescriptions for succouring sick " and
" the pen flower doctor mirror " of the Qing Dynasty, shows that pueraria lobata, hoveniae semoveniae semen, cape jasmine have good nourishing and protecting liver effect.Pueraria lobata is a kind of traditional medicine
Homologous plant is eaten, taste is sweet, cool and tasty, research shows that there is kudzu root flavone powerful anti-oxidant and promotion hepatotoxin to be metabolized
Effect.Hoveniae semoveniae semen is the drying and ripening of Rhamnaceae plant with the fruits and seeds of meat carpopodium, is traditional antialcoholic drug simply, China
There is related record in successive dynasties medicine writings, such as " Tang materia medica " of the Tang Dynasty, supplement to Thousand Golden Prescriptions, " book on Chinese herbal medicine is just read " of the Qing Dynasty, " book on Chinese herbal medicine is standby
Want ", chemical component includes saponin(e, glucosides, flavone compound etc., and pharmacological action is mainly Antialcoholic liver-protecting.Primarily directed to
Nourishing and protecting liver protects hepatic injury caused by ethyl alcohol, reduces the concentration of alcohol in blood.Cape jasmine is the drying of madder wort cape jasmine
Ripening fruits, Chinese medicine think that it has the effect of removing pattogenic heat from the blood and toxic material from the body, clearing heat and promoting diuresis, modern research shows that cape jasmine has liver protection, anti-oxidant
And other effects.In addition, being directed to nourishing and protecting liver, hepatic injury caused by ethyl alcohol is protected, reduces the concentration of alcohol in blood, pueraria lobata, trifoliate orange Dulcis
Son can accelerating alcohol decompose, inhibit absorption of the stomach to alcohol, it is the traditional Chinese medicine of two tastes integration of drinking and medicinal herbs good to stomach.
Summary of the invention
For above-mentioned status, the object of the present invention is to provide oxidation resistant pulvis of a kind of natural liver protection and preparation method thereof.
The purpose of the present invention is realized at least through one of following technical solution.
The present invention provides a kind of pulvis with liver protection anti-oxidation efficacy according to theory of traditional Chinese medical science, anti-especially suitable for demand
The crowd of oxidation, liver protection, the crowd especially often to drink.
The object of the invention is also to provide a kind of preparation methods of pulvis with liver protection anti-oxidation efficacy.
A kind of preparation method of the oxidation resistant pulvis of natural liver protection provided by the invention, includes the following steps:
(1) fresh clam is taken out into meat, removes internal organ, the moisture on surface is cleaned and removed to washing (), and mashing handles to obtain
Clam meat gruel mixes clam meat gruel with water, is uniformly mixed, obtains mixed liquor；
(2) protease is added in step (1) described mixed liquor, heating carries out enzyme digestion reaction, then carries out destroy the enzyme treatment, is centrifuged
Supernatant is taken, freeze-drying obtains clam enzymolysis protein peptide；
(3) pueraria lobata is clayed into power, is sieved, obtains pueraria lobata powder, the pueraria lobata powder is added to the water, be uniformly mixed, then
Heating carries out decoction processing, stands, and filters, and revolving obtains pueraria lobata water extract (Flavonoid substances) after freeze-drying；
(4) hoveniae semoveniae semen is clayed into power, is sieved, obtains hoveniae semoveniae semen powder；The hoveniae semoveniae semen powder is added to the water, mixing is equal
Even, then heating carries out decoction processing, stands, and filters, and revolving obtains hoveniae semoveniae semen water extract (flavonoids object after freeze-drying
Matter)；
(5) cape jasmine is clayed into power, is sieved, obtains Cape Jasmine Fruit, the Cape Jasmine Fruit is added to the water, be uniformly mixed, then
Heated, filtered, revolving obtains cape jasmine water extract (terpene substances) after freeze-drying；
(6) by step (2) the clam enzymolysis protein peptide, step (3) the pueraria lobata water extract, step (4) the hoveniae semoveniae semen water
Extract, step (5) the cape jasmine water extract are uniformly mixed with corrigent, obtain mixture, then the mixture is sieved, obtains
To the oxidation resistant pulvis of the natural liver protection.
Further, the rate of step (1) the mashing processing is 18000-20000 r/min；It is beaten the time of processing
For 3-5 min；The protein content of the clam meat gruel is greater than 14wt%；The solid-liquid ratio of the clam meat gruel and water is 1:8-1:12
g/mL。
Preferably, the solid-liquid ratio of the protein content of step (1) the clam meat gruel and water is 1:10 g/mL.
Preferably, step (1) the clam meat gruel can be stored spare under the conditions of -20 DEG C.
Further, step (2) protease is neutral proteinase；The quality of step (2) described protease is step
(1) 0.8-1.2wt% of the protein content quality of the clam meat gruel；The temperature of step (2) described enzyme digestion reaction is 53-57
DEG C, the time of enzyme digestion reaction is 3.5-4 h, and the pH of enzyme digestion reaction is 6.8-7.2.
Preferably, the quality of step (2) described protease is the 1wt% of the protein content of step (1) described clam meat gruel.
Preferably, the temperature of step (2) described enzyme digestion reaction is 55 DEG C, and the time of enzyme digestion reaction is 4 h, enzyme digestion reaction
PH is 7.0.
Preferably, in step (2) described enzyme digestion reaction, concentration can be added dropwise with side border ring and tieed up for the NaOH solution of 0.1M
The pH value for holding the mixed liquor is 7.0.
Further, the temperature of step (2) described destroy the enzyme treatment is 90-100 DEG C, and the time of destroy the enzyme treatment is 10-20
min。
Preferably, the temperature of step (2) described destroy the enzyme treatment is 100 DEG C, and the time of destroy the enzyme treatment is 10 min.
Preferably, the revolving speed of step (2) described centrifugation is 8000 rpm, and the temperature of centrifugation is 4 DEG C, and the time of centrifugation is 20
min。
Further, the slot size of step (3) described sieving is 60 mesh；The mass volume ratio of the pueraria lobata powder and water
For 1:180-1:250 g/mL；The temperature for decocting processing is 95-100 DEG C, and the time for decocting processing is 4-6 min；
The time of the standing is 4-6 min.
Preferably, step (3) temperature for decocting processing is 100 DEG C.Preferably, step (3) decoction is handled
Time is 5 min.
Preferably, the mass volume ratio of step (3) the pueraria lobata powder and water is 1:200 g/mL.
Preferably, the time of step (3) described standing is 5 min.
Further, the slot size of step (4) described sieving is 60 mesh；The quality volume of the hoveniae semoveniae semen powder and water
Than for 1:180-1:250 g/mL；The temperature for decocting processing is 95-100 DEG C, and the time for decocting processing is 4-6
min；The time of the standing is 4-6 min.
Preferably, step (4) temperature for decocting processing is 100 DEG C.Preferably, step (4) decoction is handled
Time is 5 min.
Preferably, the mass volume ratio of step (4) the hoveniae semoveniae semen powder and water is 1:200 g/mL.
Preferably, the time of step (4) described standing is 5 min.
Further, the slot size of step (5) described sieving is 60 mesh；The mass volume ratio of the Cape Jasmine Fruit and water
For 1:6-1:8 g/mL；The temperature of the heat treatment is 80-85 DEG C, and the time of the heat treatment is 3-3.5 h.
Preferably, the mass volume ratio of step (5) Cape Jasmine Fruit and water is 1:7 g/mL；The temperature for decocting processing
Degree is 85 DEG C, and the time for decocting processing is 3 h.
Further, in step (6), according to the mass fraction,
1-3 parts of clam enzymolysis protein peptide；
1-2 parts of pueraria lobata water extract；
1-2 parts of hoveniae semoveniae semen water extract；
1-2 parts of cape jasmine water extract.
Preferably, in step (6), according to the mass fraction,
1 part of clam enzymolysis protein peptide；
1 part of pueraria lobata water extract；
1 part of hoveniae semoveniae semen water extract；
1 part of cape jasmine water extract.
Further, step (6) corrigent is one or more of lemon powder, orange powder and steviol glycoside；It is described
The quality of corrigent accounts for the 0-0.37wt% of the mixture quality；The slot size of the sieving is 80 mesh.
Preferably, the corrigent be orange powder and steviol glycoside one or both of.
Preferably, when the corrigent selects orange powder, the quality of the corrigent accounts for the mixture quality
0.15%-0.2%；Further preferably 0.2%.
Preferably, when the corrigent selects steviol glycoside, the quality of the corrigent accounts for the mixture quality
0.13-0.17%；Further preferably 0.15%.
It is further preferred that the orange powder quality accounts for when the corrigent selects orange powder and steviol glycoside simultaneously
The 0.2% of the mixture quality, the steviol glycoside quality account for the 0.15% of the mixture quality.
The present invention provides a kind of oxidation resistant pulvis of the natural liver protection as made from above-mentioned preparation method.
The anti-oxidant pulvis of natural liver protection provided by the invention is according to theory of traditional Chinese medical science and to combine document report, reasonable compatibility
It is obtained.
Compared with prior art, the invention has the advantages that and the utility model has the advantages that
(1) the oxidation resistant pulvis of natural liver protection provided by the invention is that have antioxidation same according to made from theory of traditional Chinese medical science
When with the protective effect to liver, suitable for being taken to anti-oxidant, liver protection crowd in need, the people especially often to drink
Group.
Detailed description of the invention
Fig. 1 is the result display diagram of the sensory evaluation of compound product made from comparative example 1；
Fig. 2 is the result display diagram of the sensory evaluation of compound product made from comparative example 2；
Fig. 3 is the result display diagram of the sensory evaluation of compound product made from comparative example 3；
Fig. 4 is the result display diagram of the sensory evaluation of compound product made from comparative example 4；
Fig. 5 is the result display diagram of the sensory evaluation of compound product made from comparative example 5；
Fig. 6 is the result display diagram of the sensory evaluation of compound product made from comparative example 6；
Fig. 7 is the result display diagram of the sensory evaluation of the oxidation resistant pulvis of natural liver protection made from embodiment 1；
Fig. 8 is the particle size distribution figure of the oxidation resistant pulvis of natural liver protection made from embodiment 1.
Specific embodiment
Specific implementation of the invention is described further below in conjunction with attached drawing and example, but implementation and protection of the invention
It is without being limited thereto.If it is existing to be that those skilled in the art can refer to it is noted that there is the not special process of detailed description below
Technology realize or understand.Reagents or instruments used without specified manufacturer, be considered as can by it is commercially available be commercially available it is normal
Advise product.
Comparative example 1
A kind of preparation method of compound product, includes the following steps:
(1) clam is taken into meat, removes internal organ, clean and remove the moisture on surface, mashing handles to obtain clam meat gruel, mashing processing
Revolving speed be 18000 r/min, be beaten processing time be 4 min；The clam meat gruel is added to the water, clam meat gruel and water
Solid-liquid ratio be 1:10 g/mL, be uniformly mixed, obtain mixed liquor；
(2) protease (neutral proteinase, Neutral protease) is added in step (1) described mixed liquor, protease
Quality is step (1) the clam meat gruel protein content (mass fraction of the protein content of clam meat gruel is 14.59%)
1.0wt%, heating carry out enzyme digestion reaction, and the temperature of enzyme digestion reaction is 55 DEG C, and the time of enzyme digestion reaction is 4 h, in enzyme digestion reaction,
The pH value of mixed liquor maintain 7.0(be added dropwise 0.1 M NaOH solution maintain), then carry out destroy the enzyme treatment (temperature be 100 DEG C, when
Between be 10 min), be 4 DEG C in temperature, revolving speed is centrifuged 20 min and takes supernatant under conditions of being 8000 rpm, and freeze-drying obtains
Clam enzymolysis protein peptide；
(3) pueraria lobata is clayed into power, sieving (slot size is 60 mesh) obtains pueraria lobata powder, then adds the pueraria lobata powder
Entering in the ethanol solution that concentration of volume percent is 41%, the mass volume ratio of pueraria lobata powder and ethanol solution is 1:44 g/mL,
Then ultrasonic extraction processing (16 min of ultrasound under the conditions of 230 W, 40 Khz, 40 DEG C) are carried out, filters to take filtrate, obtains pueraria lobata
Alcohol extract, revolving, freeze-drying after obtain pueraria lobata alcohol extract；The pueraria lobata powder is added to the water, pueraria lobata powder and water
Mass volume ratio be 1:200 g/mL；It is uniformly mixed, then heating carries out decoction processing, and the temperature for decocting processing is 100 DEG C,
Decocting the processing time is 5 min, stands 5 min, and filtering obtains pueraria lobata water extract after revolving, freeze-drying；
(4) hoveniae semoveniae semen is clayed into power, sieving (slot size is 60 mesh) obtains hoveniae semoveniae semen powder, then by the hoveniae semoveniae semen
Powder is added in the ethanol solution that concentration of volume percent is 41%, and the mass volume ratio of hoveniae semoveniae semen powder and ethanol solution is 1:
Then 44 g/mL carry out ultrasonic extraction processing (16 min of ultrasound under the conditions of 230 W, 40 Khz, 40 DEG C), filter to take filtrate,
The alcohol extract of hoveniae semoveniae semen is obtained, obtains hoveniae semoveniae semen alcohol extract after revolving, freeze-drying；Water is added in the hoveniae semoveniae semen powder
In, the mass volume ratio of hoveniae semoveniae semen powder and water is 1:200 g/mL, is uniformly mixed, and then heating carries out decoction processing, is decocted
The temperature of processing is 100 DEG C, and decocting the processing time is 5 min, stands 5 min, and filtering obtains trifoliate orange Dulcis after revolving, freeze-drying
Sub- water extract；
(5) cape jasmine is clayed into power, sieving (slot size is 60 mesh) obtains Cape Jasmine Fruit, then adds the Cape Jasmine Fruit
Enter in the ethanol solution that concentration of volume percent is 95%, the mass volume ratio of the Cape Jasmine Fruit and ethanol solution is 1:20 g/
Then mL carries out ultrasonic extraction processing (30 min of ultrasound under the conditions of 140 W, 45 Khz, 40 DEG C), filters to take filtrate, obtain
The alcohol extract of cape jasmine obtains cape jasmine alcohol extract after revolving, freeze-drying；The Cape Jasmine Fruit is added to the water, Cape Jasmine Fruit
Mass volume ratio with water is 1:7 g/mL, is uniformly mixed, and then heating carries out decoction processing, and the temperature for decocting processing is 85
DEG C, the time for decocting processing is 3 h, and filtering obtains cape jasmine water extract after revolving, freeze-drying；
(6) 6 parts of step (2) clam enzymolysis protein peptides are described with step (3) the pueraria lobata alcohol extract, step (3) respectively
Pueraria lobata water extract, step (4) the hoveniae semoveniae semen alcohol extract, step (4) the hoveniae semoveniae semen water extract and step (5) described cape jasmine
Alcohol extract and step (5) the cape jasmine water extract are mixed according to mass ratio 1:1, obtain 6 parts of mixtures after mixing, so
6 parts of mixtures are sieved (slot size is 80 mesh) respectively afterwards, obtain 6 parts of compound products.It will be made from comparative example 1
6 parts of compound products are uniformly mixed with water according to solid-liquid ratio 1:30 g/mL brew respectively, then carry out the sensory evaluation (mark of evaluation
It is quasi- as shown in table 1), as a result as shown in Figure 1.Pueraria lobota alcohol, Ge Shui, trifoliate orange alcohol, Zhi Shui, Cape jasmine alcohol and Cape jasmine water respectively indicate clam enzyme in Fig. 1
The compound product that is uniformly mixed with pueraria lobata alcohol extract of solution protein peptides, clam enzymolysis protein peptide are uniformly mixed with pueraria lobata water extract
Compound product that obtained compound product, clam enzymolysis protein peptide are uniformly mixed with hoveniae semoveniae semen alcohol extract, clam enzymatic hydrolysis egg
Compound product that white peptide is uniformly mixed with hoveniae semoveniae semen water extract, clam enzymolysis protein peptide are uniformly mixed with cape jasmine alcohol extract
To the compound product that is uniformly mixed with cape jasmine water extract of compound product and clam enzymolysis protein peptide.
Comparative example 2
A kind of preparation method of compound product, includes the following steps:
(1) clam is taken into meat, removes internal organ, clean and remove the moisture on surface, mashing handles to obtain clam meat gruel, mashing processing
Revolving speed be 18000 r/min, be beaten processing time be 4 min；The clam meat gruel is added to the water, clam meat gruel and water
Solid-liquid ratio be 1:10g/mL, be uniformly mixed, obtain mixed liquor；
(2) protease (neutral proteinase, Neutral protease) is added in step (1) described mixed liquor, protease
Quality is the 1.0wt% of step (1) the clam meat gruel protein content (the albumen quality score of clam meat gruel is 14.59%), is added
Heat carries out enzyme digestion reaction, and the temperature of enzyme digestion reaction is 55 DEG C, and the time of enzyme digestion reaction is 4 h, in enzyme digestion reaction, the pH of mixed liquor
Value maintains 7.0(and the maintenance of 0.1 M NaOH solution is added dropwise), then carrying out destroy the enzyme treatment, (temperature is 100 DEG C, the time 10
It min), is 4 DEG C in temperature, revolving speed is centrifuged 20 min and takes supernatant under conditions of being 8000 rpm, freeze-drying obtains clam enzyme
Solve protein peptides；
(3) pueraria lobata is clayed into power, sieving (slot size is 60 mesh) obtains pueraria lobata powder, then adds the pueraria lobata powder
Entering in the ethanol solution that concentration of volume percent is 41%, the mass volume ratio of pueraria lobata powder and ethanol solution is 1:44 g/mL,
Then ultrasonic extraction processing (16 min of ultrasound under the conditions of 230 W, 40 Khz, 40 DEG C) are carried out, filters to take filtrate, obtains pueraria lobata
Alcohol extract, revolving, freeze-drying after obtain pueraria lobata alcohol extract；The pueraria lobata powder is added to the water, pueraria lobata powder and water
Mass volume ratio be 1:200 g/mL；It is uniformly mixed, then heating carries out decoction processing, and the temperature for decocting processing is 100 DEG C,
Decocting the processing time is 5 min, stands 5 min, and filtering obtains pueraria lobata water extract after revolving, freeze-drying；
(4) hoveniae semoveniae semen is clayed into power, sieving (slot size is 60 mesh) obtains hoveniae semoveniae semen powder, then by the hoveniae semoveniae semen
Powder is added in the ethanol solution that concentration of volume percent is 41%, and the mass volume ratio of hoveniae semoveniae semen powder and ethanol solution is 1:
Then 44 g/mL carry out ultrasonic extraction processing (16 min of ultrasound under the conditions of 230 W, 40 Khz, 40 DEG C), filter to take filtrate,
The alcohol extract of hoveniae semoveniae semen is obtained, obtains hoveniae semoveniae semen alcohol extract after revolving, freeze-drying；Water is added in the hoveniae semoveniae semen powder
In, the mass volume ratio of hoveniae semoveniae semen powder and water is 1:200 g/mL, is uniformly mixed, and then heating carries out decoction processing, is decocted
The temperature of processing is 100 DEG C, and decocting the processing time is 5 min, stands 5 min, and filtering obtains trifoliate orange Dulcis after revolving, freeze-drying
Sub- water extract；
(5) cape jasmine is clayed into power, sieving (slot size is 60 mesh) obtains Cape Jasmine Fruit, then adds the Cape Jasmine Fruit
Enter in the ethanol solution that concentration of volume percent is 95%, the mass volume ratio of the Cape Jasmine Fruit and ethanol solution is 1:20 g/
Then mL carries out ultrasonic extraction processing (30 min of ultrasound under the conditions of 140 W, 45 Khz, 40 DEG C), filters to take filtrate, obtain
The alcohol extract of cape jasmine obtains cape jasmine alcohol extract after revolving, freeze-drying；The Cape Jasmine Fruit is added to the water, Cape Jasmine Fruit
Mass volume ratio with water is 1:7 g/mL, is uniformly mixed, and then heating carries out decoction processing, and the temperature for decocting processing is 85
DEG C, the time for decocting processing is 3 h, and filtering obtains cape jasmine water extract after revolving, freeze-drying；
(6) by 6 parts of step (2) clam enzymolysis protein peptides respectively with step (3) the pueraria lobata alcohol extract, pueraria lobata water extract,
Step (4) the hoveniae semoveniae semen alcohol extract, hoveniae semoveniae semen water extract and step (5) the cape jasmine alcohol extract, cape jasmine water extract are according to matter
Amount is mixed than 1:1, then the lemon powder that addition mass fraction is 0.2% respectively, obtains 6 parts of mixtures after mixing, then
6 parts of mixtures are sieved (slot size is 80 mesh) respectively, obtain 6 parts of compound products.Compounding is made in comparative example 2
Product is uniformly mixed with water according to solid-liquid ratio 1:30 g/mL and brews, and then carries out the sensory evaluation (standard of evaluation such as 1 institute of table
Show), as a result as shown in Figure 2.In Fig. 2 Pueraria lobota alcohol, Ge Shui, trifoliate orange alcohol, Zhi Shui, Cape jasmine alcohol and Cape jasmine water respectively indicate clam enzymolysis protein peptide with
Pueraria lobata alcohol extract be uniformly mixed obtained compound product, clam enzymolysis protein peptide and pueraria lobata water extract it is uniformly mixed obtain compound
Compound product, clam enzymolysis protein peptide and the trifoliate orange Dulcis that product, clam enzymolysis protein peptide are uniformly mixed with hoveniae semoveniae semen alcohol extract
Sub- water extract be uniformly mixed obtained compound product, clam enzymolysis protein peptide and cape jasmine alcohol extract it is uniformly mixed obtain compound production
The compound product that product and clam enzymolysis protein peptide are uniformly mixed with cape jasmine water extract.
Comparative example 3
A kind of preparation method of compound product, includes the following steps:
(1) clam is taken into meat, removes internal organ, clean and remove the moisture on surface, mashing handles to obtain clam meat gruel, mashing processing
Revolving speed be 18000 r/min, be beaten processing time be 4 min；The clam meat gruel is added to the water, clam meat gruel and water
Solid-liquid ratio be 1:10 g/mL, be uniformly mixed, obtain mixed liquor；
(2) protease (neutral proteinase, Neutral protease) is added in step (1) described mixed liquor, protease
Quality is the 1.0wt% of the protein content (the albumen quality score of clam meat gruel is 14.59%) of step (1) the clam meat gruel,
Heating carries out enzyme digestion reaction, and the temperature of enzyme digestion reaction is 55 DEG C, and the time of enzyme digestion reaction is 4 h, in enzyme digestion reaction, mixed liquor
PH value maintains 7.0(and the maintenance of 0.1 M NaOH solution is added dropwise), then carrying out destroy the enzyme treatment, (temperature is 100 DEG C, the time 10
It min), is 4 DEG C in temperature, revolving speed is centrifuged 20 min and takes supernatant under conditions of being 8000 rpm, freeze-drying obtains clam enzyme
Solve protein peptides；
(3) pueraria lobata is clayed into power, sieving (slot size is 60 mesh) obtains pueraria lobata powder, then adds the pueraria lobata powder
Entering in the ethanol solution that concentration of volume percent is 41%, the mass volume ratio of pueraria lobata powder and ethanol solution is 1:44 g/mL,
Then ultrasonic extraction processing (16 min of ultrasound under the conditions of 230 W, 40 Khz, 40 DEG C) are carried out, filters to take filtrate, obtains pueraria lobata
Alcohol extract, revolving, freeze-drying after obtain pueraria lobata alcohol extract；The pueraria lobata powder is added to the water, pueraria lobata powder and water
Mass volume ratio be 1:200 g/mL；It is uniformly mixed, then heating carries out decoction processing, and the temperature for decocting processing is 100 DEG C,
Decocting the processing time is 5 min, stands 5 min, and filtering obtains pueraria lobata water extract after revolving, freeze-drying；
(4) hoveniae semoveniae semen is clayed into power, sieving (slot size is 60 mesh) obtains hoveniae semoveniae semen powder, then by the hoveniae semoveniae semen
Powder is added in the ethanol solution that concentration of volume percent is 41%, and the mass volume ratio of hoveniae semoveniae semen powder and ethanol solution is 1:
Then 44 g/mL carry out ultrasonic extraction processing (16 min of ultrasound under the conditions of 230 W, 40 Khz, 40 DEG C), filter to take filtrate,
The alcohol extract of hoveniae semoveniae semen is obtained, obtains hoveniae semoveniae semen alcohol extract after revolving, freeze-drying；Water is added in the hoveniae semoveniae semen powder
In, the mass volume ratio of hoveniae semoveniae semen powder and water is 1:200 g/mL, is uniformly mixed, and then heating carries out decoction processing, is decocted
The temperature of processing is 100 DEG C, and decocting the processing time is 5 min, stands 5 min, and filtering obtains trifoliate orange Dulcis after revolving, freeze-drying
Sub- water extract；
(5) cape jasmine is clayed into power, sieving (slot size is 60 mesh) obtains Cape Jasmine Fruit, then adds the Cape Jasmine Fruit
Enter in the ethanol solution that concentration of volume percent is 95%, the mass volume ratio of the Cape Jasmine Fruit and ethanol solution is 1:20 g/
Then mL carries out ultrasonic extraction processing (30 min of ultrasound under the conditions of 140 W, 45 Khz, 40 DEG C), filters to take filtrate, obtain
The alcohol extract of cape jasmine obtains cape jasmine alcohol extract after revolving, freeze-drying；The Cape Jasmine Fruit is added to the water, Cape Jasmine Fruit
Mass volume ratio with water is 1:7 g/mL, is uniformly mixed, and then heating carries out decoction processing, and the temperature for decocting processing is 85
DEG C, the time for decocting processing is 3 h, and filtering obtains cape jasmine water extract after revolving, freeze-drying；
(6) by 6 parts of step (2) clam enzymolysis protein peptides respectively with step (3) the pueraria lobata alcohol extract, pueraria lobata water extract,
Step (4) the hoveniae semoveniae semen alcohol extract, hoveniae semoveniae semen water extract and step (5) the cape jasmine alcohol extract, cape jasmine water extract are pressed respectively
It is mixed according to mass ratio 1:1, then the orange powder that addition mass fraction is 0.2% respectively, obtains 6 parts of mixtures after mixing,
Then 6 parts of mixtures are sieved (slot size is 80 mesh) respectively, obtain 6 parts of compound products.Comparative example 3 is made
Compound product is uniformly mixed with water according to solid-liquid ratio 1:30 g/mL and brews, and then carries out the sensory evaluation (standard of evaluation such as table 1
It is shown), as a result as shown in Figure 3.Pueraria lobota alcohol, Ge Shui, trifoliate orange alcohol, Zhi Shui, Cape jasmine alcohol and Cape jasmine water respectively indicate clam enzymolysis protein peptide in Fig. 3
The compound product that is uniformly mixed with pueraria lobata alcohol extract, clam enzymolysis protein peptide are answered with what pueraria lobata water extract was uniformly mixed
Compound product, clam enzymolysis protein peptide and the trifoliate orange being uniformly mixed with product, clam enzymolysis protein peptide with hoveniae semoveniae semen alcohol extract
Dulcis water extract be uniformly mixed obtained compound product, clam enzymolysis protein peptide and cape jasmine alcohol extract it is uniformly mixed obtain compound
The compound product that product and clam enzymolysis protein peptide are uniformly mixed with cape jasmine water extract.
Comparative example 4
A kind of preparation method of compound product, includes the following steps:
(1) clam is taken into meat, removes internal organ, clean and remove the moisture on surface, mashing handles to obtain clam meat gruel, mashing processing
Revolving speed be 18000 r/min, be beaten processing time be 4 min；The clam meat gruel is added to the water, clam meat gruel and water
Solid-liquid ratio be 1:10 g/mL, be uniformly mixed, obtain mixed liquor；
(2) protease (neutral proteinase, Neutral protease) is added in step (1) described mixed liquor, protease
Quality is the 1.0wt% of step (1) the clam meat gruel protein content (the albumen quality score of clam meat gruel is 14.59%), is added
Heat carries out enzyme digestion reaction, and the temperature of enzyme digestion reaction is 55 DEG C, and the time of enzyme digestion reaction is 4 h, in enzyme digestion reaction, the pH of mixed liquor
Value maintains 7.0(and the maintenance of 0.1 M NaOH solution is added dropwise), then carrying out destroy the enzyme treatment, (temperature is 100 DEG C, the time 10
It min), is 4 DEG C in temperature, revolving speed is centrifuged 20 min and takes supernatant under conditions of being 8000 rpm, freeze-drying obtains clam enzyme
Solve protein peptides；
(3) pueraria lobata is clayed into power, sieving (slot size is 60 mesh) obtains pueraria lobata powder, then adds the pueraria lobata powder
Entering in the ethanol solution that concentration of volume percent is 41%, the mass volume ratio of pueraria lobata powder and ethanol solution is 1:44 g/mL,
Then ultrasonic extraction processing (16 min of ultrasound under the conditions of 230 W, 40 Khz, 40 DEG C) are carried out, filters to take filtrate, obtains pueraria lobata
Alcohol extract, revolving, freeze-drying after obtain pueraria lobata alcohol extract；The pueraria lobata powder is added to the water, pueraria lobata powder and water
Mass volume ratio be 1:200 g/mL；It is uniformly mixed, then heating carries out decoction processing, and the temperature for decocting processing is 100 DEG C,
Decocting the processing time is 5 min, stands 5 min, and filtering obtains pueraria lobata water extract after revolving, freeze-drying；
(4) hoveniae semoveniae semen is clayed into power, sieving (slot size is 60 mesh) obtains hoveniae semoveniae semen powder, then by the hoveniae semoveniae semen
Powder is added in the ethanol solution that concentration of volume percent is 41%, and the mass volume ratio of hoveniae semoveniae semen powder and ethanol solution is 1:
Then 44 g/mL carry out ultrasonic extraction processing (16 min of ultrasound under the conditions of 230 W, 40 Khz, 40 DEG C), filter to take filtrate,
The alcohol extract of hoveniae semoveniae semen is obtained, obtains hoveniae semoveniae semen alcohol extract after revolving, freeze-drying；Water is added in the hoveniae semoveniae semen powder
In, the mass volume ratio of hoveniae semoveniae semen powder and water is 1:200 g/mL, is uniformly mixed, and then heating carries out decoction processing, is decocted
The temperature of processing is 100 DEG C, and decocting the processing time is 5 min, stands 5 min, and filtering obtains trifoliate orange Dulcis after revolving, freeze-drying
Sub- water extract；
(5) cape jasmine is clayed into power, sieving (slot size is 60 mesh) obtains Cape Jasmine Fruit, then adds the Cape Jasmine Fruit
Enter in the ethanol solution that concentration of volume percent is 95%, the mass volume ratio of the Cape Jasmine Fruit and ethanol solution is 1:20 g/
Then mL carries out ultrasonic extraction processing (30 min of ultrasound under the conditions of 140 W, 45 Khz, 40 DEG C), filters to take filtrate, obtain
The alcohol extract of cape jasmine obtains cape jasmine alcohol extract after revolving, freeze-drying；The Cape Jasmine Fruit is added to the water, Cape Jasmine Fruit
Mass volume ratio with water is 1:7 g/mL, is uniformly mixed, and then heating carries out decoction processing, and the temperature for decocting processing is 85
DEG C, the time for decocting processing is 3 h, and filtering obtains cape jasmine water extract after revolving, freeze-drying；
(6) step (2) the clam enzymolysis protein peptide and step (3) the pueraria lobata water extract are mixed, the clam digests egg
The mass ratio of white peptide and the pueraria lobata water extract is 1:1, obtains initial mixture；Then 6 parts of initial mixtures are distinguished
With step (3) the pueraria lobata alcohol extract, step (4) the hoveniae semoveniae semen alcohol extract, step (4) the hoveniae semoveniae semen water extract and step
(5) the cape jasmine alcohol extract, step (5) the cape jasmine water extract are mixed according to mass ratio 2:1 respectively, then add matter respectively
The orange powder that score is 0.2% is measured, obtains 5 parts of mixtures after mixing, then by 5 parts of mixtures sieving (slot size
For 80 mesh), obtain 5 parts of compound products.Compound product is made in comparative example 4 to mix according to solid-liquid ratio 1:30 g/mL with water
It uniformly brews, then carries out sensory evaluation (standard of evaluation is as shown in table 1), as a result as shown in Figure 4.Pueraria lobota alcohol in Fig. 4, trifoliate orange alcohol,
Zhi Shui, Cape jasmine alcohol and Cape jasmine water respectively indicate compound product, the preliminary mixing that initial mixture is uniformly mixed with pueraria lobata alcohol extract
Compound product that object is uniformly mixed with hoveniae semoveniae semen alcohol extract, initial mixture are uniformly mixed with hoveniae semoveniae semen water extract
The compound product and initial mixture and cape jasmine water extract that compound product, initial mixture are uniformly mixed with cape jasmine alcohol extract
It is uniformly mixed obtained compound product.
Comparative example 5
A kind of preparation method of compound product, includes the following steps:
(1) clam is taken into meat, removes internal organ, clean and remove the moisture on surface, mashing handles to obtain clam meat gruel, mashing processing
Revolving speed be 18000 r/min, be beaten processing time be 4 min；The clam meat gruel is added to the water, clam meat gruel and water
Solid-liquid ratio be 1:10 g/mL, be uniformly mixed, obtain mixed liquor；
(2) protease (neutral proteinase, Neutral protease) is added in step (1) described mixed liquor, protease
Quality is the 1.0wt% of step (1) the clam meat gruel protein content (the albumen quality score of clam meat gruel is 14.59%), is added
Heat carries out enzyme digestion reaction, and the temperature of enzyme digestion reaction is 55 DEG C, and the time of enzyme digestion reaction is 4 h, in enzyme digestion reaction, the pH of mixed liquor
Value maintains 7.0(and the maintenance of 0.1 M NaOH solution is added dropwise), then carrying out destroy the enzyme treatment, (temperature is 100 DEG C, the time 10
It min), is 4 DEG C in temperature, revolving speed is centrifuged 20 min and takes supernatant under conditions of being 8000 rpm, freeze-drying obtains clam enzyme
Solve protein peptides；
(3) pueraria lobata is clayed into power, sieving (slot size is 60 mesh) obtains pueraria lobata powder, then adds the pueraria lobata powder
Entering in the ethanol solution that concentration of volume percent is 41%, the mass volume ratio of pueraria lobata powder and ethanol solution is 1:44 g/mL,
Then ultrasonic extraction processing (16 min of ultrasound under the conditions of 230 W, 40 Khz, 40 DEG C) are carried out, filters to take filtrate, obtains pueraria lobata
Alcohol extract, revolving, freeze-drying after obtain pueraria lobata alcohol extract；The pueraria lobata powder is added to the water, pueraria lobata powder and water
Mass volume ratio be 1:200 g/mL；It is uniformly mixed, then heating carries out decoction processing, and the temperature for decocting processing is 100 DEG C,
Decocting the processing time is 5 min, stands 5 min, and filtering obtains pueraria lobata water extract after revolving, freeze-drying；
(4) hoveniae semoveniae semen is clayed into power, sieving (slot size is 60 mesh) obtains hoveniae semoveniae semen powder, then by the hoveniae semoveniae semen
Powder is added in the ethanol solution that concentration of volume percent is 41%, and the mass volume ratio of hoveniae semoveniae semen powder and ethanol solution is 1:
Then 44 g/mL carry out ultrasonic extraction processing (16 min of ultrasound under the conditions of 230 W, 40 Khz, 40 DEG C), filter to take filtrate,
The alcohol extract of hoveniae semoveniae semen is obtained, obtains hoveniae semoveniae semen alcohol extract after revolving, freeze-drying；Water is added in the hoveniae semoveniae semen powder
In, the mass volume ratio of hoveniae semoveniae semen powder and water is 1:200 g/mL, is uniformly mixed, and then heating carries out decoction processing, is decocted
The temperature of processing is 100 DEG C, and decocting the processing time is 5 min, stands 5 min, and filtering obtains trifoliate orange Dulcis after revolving, freeze-drying
Sub- water extract；
(5) cape jasmine is clayed into power, sieving (slot size is 60 mesh) obtains Cape Jasmine Fruit, then adds the Cape Jasmine Fruit
Enter in the ethanol solution that concentration of volume percent is 95%, the mass volume ratio of the Cape Jasmine Fruit and ethanol solution is 1:20 g/
Then mL carries out ultrasonic extraction processing (30 min of ultrasound under the conditions of 140 W, 45 Khz, 40 DEG C), filters to take filtrate, obtain
The alcohol extract of cape jasmine obtains cape jasmine alcohol extract after revolving, freeze-drying；The Cape Jasmine Fruit is added to the water, Cape Jasmine Fruit
Mass volume ratio with water is 1:7 g/mL, is uniformly mixed, and then heating carries out decoction processing, and the temperature for decocting processing is 85
DEG C, the time for decocting processing is 3 h, and filtering obtains cape jasmine water extract after revolving, freeze-drying；
(6) step (2) the clam enzymolysis protein peptide, step (3) the pueraria lobata water extract, step (5) the cape jasmine water are mentioned
The mass ratio of object mixing, the clam enzymolysis protein peptide, pueraria lobata water extract and cape jasmine water extract is 1:1:1, is tentatively mixed
Object；Then by 6 parts of initial mixtures respectively with step (3) the pueraria lobata alcohol extract, step (4) the hoveniae semoveniae semen alcohol extract, step
Suddenly (4) described hoveniae semoveniae semen water extract and step (5) the cape jasmine alcohol extract are mixed according to mass ratio 3:0.5, then are added respectively
Adding mass fraction is 0.2% orange powder, obtains 4 parts of mixtures after mixing, then by 4 parts of mixtures sieving (sieve pore
Size is 80 mesh), obtain 4 parts of compound products.Compound product is made according to solid-liquid ratio 1:30 g/mL and water in comparative example 5
It is uniformly mixed and brews, then carry out sensory evaluation (standard of evaluation is as shown in table 1), as a result as shown in Figure 5.Pueraria lobota alcohol, trifoliate orange in Fig. 5
Alcohol, Zhi Shui and Cape jasmine alcohol respectively indicate compound product, the initial mixture that initial mixture is uniformly mixed with pueraria lobata alcohol extract
The compound product that is uniformly mixed with hoveniae semoveniae semen alcohol extract, initial mixture are answered with what hoveniae semoveniae semen water extract was uniformly mixed
The compound product being uniformly mixed with product and initial mixture with cape jasmine alcohol extract.
Comparative example 6
A kind of preparation method of compound product, includes the following steps:
(1) clam is taken into meat, removes internal organ, clean and remove the moisture on surface, mashing handles to obtain clam meat gruel, mashing processing
Revolving speed be 18000 r/min, be beaten processing time be 4 min；The clam meat gruel is added to the water, clam meat gruel and water
Solid-liquid ratio be 1:10 g/mL, be uniformly mixed, obtain mixed liquor；
(2) protease (neutral proteinase, Neutral protease) is added in step (1) described mixed liquor, protease
Quality is the 1.0wt% of step (1) the clam meat gruel protein content (the albumen quality score of clam meat gruel is 14.59%), is added
Heat carries out enzyme digestion reaction, and the temperature of enzyme digestion reaction is 55 DEG C, and the time of enzyme digestion reaction is 4 h, in enzyme digestion reaction, the pH of mixed liquor
Value maintains 7.0(and the maintenance of 0.1 M NaOH solution is added dropwise), then carrying out destroy the enzyme treatment, (temperature is 100 DEG C, the time 10
It min), is 4 DEG C in temperature, revolving speed is centrifuged 20 min and takes supernatant under conditions of being 8000 rpm, freeze-drying obtains clam enzyme
Solve protein peptides；
(3) pueraria lobata is clayed into power, sieving (slot size is 60 mesh) obtains pueraria lobata powder, then adds the pueraria lobata powder
Entering in the ethanol solution that concentration of volume percent is 41%, the mass volume ratio of pueraria lobata powder and ethanol solution is 1:44 g/mL,
Then ultrasonic extraction processing (16 min of ultrasound under the conditions of 230 W, 40 Khz, 40 DEG C) are carried out, filters to take filtrate, obtains pueraria lobata
Alcohol extract, revolving, freeze-drying after obtain pueraria lobata alcohol extract；The pueraria lobata powder is added to the water, pueraria lobata powder and water
Mass volume ratio be 1:200 g/mL；It is uniformly mixed, then heating carries out decoction processing, and the temperature for decocting processing is 100 DEG C,
Decocting the processing time is 5 min, stands 5 min, and filtering obtains pueraria lobata water extract after revolving, freeze-drying；
(4) hoveniae semoveniae semen is clayed into power, sieving (slot size is 60 mesh) obtains hoveniae semoveniae semen powder, then by the hoveniae semoveniae semen
Powder is added in the ethanol solution that concentration of volume percent is 41%, and the mass volume ratio of hoveniae semoveniae semen powder and ethanol solution is 1:
Then 44 g/mL carry out ultrasonic extraction processing (16 min of ultrasound under the conditions of 230 W, 40 Khz, 40 DEG C), filter to take filtrate,
The alcohol extract of hoveniae semoveniae semen is obtained, obtains hoveniae semoveniae semen alcohol extract after revolving, freeze-drying；Water is added in the hoveniae semoveniae semen powder
In, the mass volume ratio of hoveniae semoveniae semen powder and water is 1:200 g/mL, is uniformly mixed, and then heating carries out decoction processing, is decocted
The temperature of processing is 100 DEG C, and decocting the processing time is 5 min, stands 5 min, and filtering obtains trifoliate orange Dulcis after revolving, freeze-drying
Sub- water extract；
(5) cape jasmine is clayed into power, sieving (slot size is 60 mesh) obtains Cape Jasmine Fruit, then adds the Cape Jasmine Fruit
Enter in the ethanol solution that concentration of volume percent is 95%, the mass volume ratio of the Cape Jasmine Fruit and ethanol solution is 1:20 g/
Then mL carries out ultrasonic extraction processing (30 min of ultrasound under the conditions of 140 W, 45 Khz, 40 DEG C), filters to take filtrate, obtain
The alcohol extract of cape jasmine obtains cape jasmine alcohol extract after revolving, freeze-drying；The Cape Jasmine Fruit is added to the water, Cape Jasmine Fruit
Mass volume ratio with water is 1:7 g/mL, is uniformly mixed, and then heating carries out decoction processing, and the temperature for decocting processing is 85
DEG C, the time for decocting processing is 3 h, and filtering obtains cape jasmine water extract after revolving, freeze-drying；
(6) step (2) the clam enzymolysis protein peptide, step (3) the pueraria lobata water extract, step (5) the cape jasmine water are mentioned
The mass ratio of object mixing, the clam enzymolysis protein peptide, the pueraria lobata water extract and the cape jasmine water extract is 1:1:1, is obtained
Initial mixture；Then by 4 parts of initial mixtures respectively with step (3) the pueraria lobata alcohol extract, step (4) the hoveniae semoveniae semen alcohol
Extract, step (4) the hoveniae semoveniae semen water extract and step (5) the cape jasmine alcohol extract are mixed according to mass ratio 1:1:1:1,
The orange powder that addition mass fraction is 0.2% respectively again, obtains 4 parts of mixtures, then by 4 parts of mixtures after mixing
Sieving (slot size is 80 mesh), obtains 4 parts of compound products.Compound product is made according to solid-liquid ratio 1:30 in comparative example 6
G/mL is uniformly mixed with water and brews, and then carries out sensory evaluation (standard of evaluation is as shown in table 1), as a result as shown in Figure 6.Fig. 6
Middle Pueraria lobota alcohol, trifoliate orange alcohol, Zhi Shui and Cape jasmine alcohol respectively indicate the compound product that initial mixture is uniformly mixed with pueraria lobata alcohol extract, just
The compound product that is uniformly mixed with hoveniae semoveniae semen alcohol extract of step mixture, initial mixture are uniformly mixed with hoveniae semoveniae semen water extract
The compound product that obtained compound product and initial mixture is uniformly mixed with cape jasmine alcohol extract.
Embodiment 1
A kind of preparation method of the oxidation resistant pulvis of natural liver protection, includes the following steps:
(1) clam is taken into meat, removes internal organ, washs and remove the moisture on surface, mashing handles to obtain clam meat gruel (clam meat gruel
Albumen quality score be 14.59%), be beaten processing revolving speed be 18000r/min, be beaten processing time be 4 min；It will be literary
Clam meat paste is mixed with water, and the solid-liquid ratio of clam meat gruel and water is 1:10 g/mL, is uniformly mixed, is obtained mixed liquor；
(2) protease (neutral proteinase, Neutral protease) is added in step (1) described mixed liquor, protease
Quality is the 1.0wt% of step (1) the clam meat gruel protein content (the albumen quality score of clam meat gruel is 14.59%), is added
Heat carries out enzyme digestion reaction, and the temperature of enzyme digestion reaction is 55 DEG C, and the time of enzyme digestion reaction is 4 h, in enzyme digestion reaction, the pH of mixed liquor
Value maintains 7.0(and the maintenance of 0.1 M NaOH solution is added dropwise), then carrying out destroy the enzyme treatment, (temperature is 100 DEG C, the time 10
It min), is 4 DEG C in temperature, revolving speed is centrifuged 20 min and takes supernatant under conditions of being 8000 rpm, freeze-drying obtains clam enzyme
Solve protein peptides；
(3) pueraria lobata is clayed into power, sieving (slot size is 60 mesh) obtains pueraria lobata powder, water is added in the pueraria lobata powder
In, the mass volume ratio of pueraria lobata powder and water is 1:200 g/mL, is uniformly mixed, and then heats and carries out decoction processing, at decoction
The temperature of reason is 100 DEG C, and decocting the processing time is 5 min, stands 5 min, is filtered, and revolving obtains pueraria lobata water after freeze-drying
Extract；
(4) hoveniae semoveniae semen is clayed into power, sieving (slot size is 60 mesh) obtains hoveniae semoveniae semen powder；By the hoveniae semoveniae semen powder
It is added to the water, the mass volume ratio of hoveniae semoveniae semen powder and water is 1:200 g/mL, is uniformly mixed, and then heating carries out at decoction
Reason, the temperature for decocting processing is 100 DEG C, and decocting the processing time is 5 min, stands 5 min, is filtered, revolving, after freeze-drying
To hoveniae semoveniae semen water extract；
(5) cape jasmine is clayed into power, sieving (slot size is 60 mesh) obtains Cape Jasmine Fruit, water is added in the Cape Jasmine Fruit
In, the mass volume ratio of Cape Jasmine Fruit and water is 1:7 g/mL, is uniformly mixed, is then heated, the temperature of heat treatment
Degree is 85 DEG C, and the time of heat treatment is 3 h, is filtered, and revolving obtains cape jasmine water extract after freeze-drying；
(6) by step (2) the clam enzymolysis protein peptide, step (3) the pueraria lobata water extract, step (4) the hoveniae semoveniae semen water
Extract, step (5) the cape jasmine water extract are uniformly mixed, the text with corrigent (selecting orange powder and steviol glycoside herein)
Clam enzymolysis protein peptide, pueraria lobata water extract, hoveniae semoveniae semen water extract and cape jasmine water extract mass ratio be 1:1:1:1, obtain mixture,
The orange powder quality accounts for the 0.2wt% of the mixture quality, and the steviol glycoside quality accounts for the mixture quality
0.1wt% obtains the oxidation resistant pulvis of the natural liver protection then by mixture sieving (slot size is 80 mesh).
(7) by step (2) the clam enzymolysis protein peptide, step (3) the pueraria lobata water extract, step (4) the trifoliate orange Dulcis
Sub- water extract, step (5) the cape jasmine water extract and corrigent (selecting orange powder and steviol glycoside herein), are uniformly mixed,
Mixture is obtained, the orange powder quality accounts for the 0.2wt% of the mixture quality, and the steviol glycoside quality accounts for the mixing
It is oxidation resistant to obtain the natural liver protection then by mixture sieving (slot size is 80 mesh) by the 0.15wt% of amount of substance
Pulvis；The clam enzymolysis protein peptide, pueraria lobata water extract, hoveniae semoveniae semen water extract and cape jasmine water extract mass ratio be 1:1:1:1.
(8) by step (2) the clam enzymolysis protein peptide, step (3) the pueraria lobata water extract, step (4) the trifoliate orange Dulcis
Sub- water extract, step (5) the cape jasmine water extract and corrigent (selecting orange powder and steviol glycoside herein) are uniformly mixed, and are obtained
To mixture, the orange powder quality accounts for the 0.2wt% of the mixture quality, and steviol glycoside quality accounts for the mixture quality
0.2wt% obtain the oxidation resistant pulvis of the natural liver protection then by mixture sieving (slot size be 80 mesh)；Institute
The mass ratio for stating clam enzymolysis protein peptide, pueraria lobata water extract, hoveniae semoveniae semen water extract and cape jasmine water extract is 1:1:1:1.
Embodiment 2
A kind of preparation method of the oxidation resistant pulvis of natural liver protection, includes the following steps:
(1) clam is taken into meat, removes internal organ, washs and remove the moisture on surface, mashing handles to obtain clam meat gruel (clam meat gruel
Albumen quality score be 14.59%), be beaten processing revolving speed be 19000 r/min, be beaten processing time be 4 min；It will
Clam meat gruel is mixed with water, and the solid-liquid ratio of clam meat gruel and water is 1:11 g/mL, is uniformly mixed, is obtained mixed liquor；
(2) protease (neutral proteinase, Neutral protease) is added in step (1) described mixed liquor, protease
Quality is the 1.2wt% of step (1) the clam meat gruel protein content (the albumen quality score of clam meat gruel is 14.59%), is added
Heat carries out enzyme digestion reaction, and the temperature of enzyme digestion reaction is 53 DEG C, and the time of enzyme digestion reaction is 4 h, in enzyme digestion reaction, the pH of mixed liquor
Value maintains 7.2(and the maintenance of 0.1 M NaOH solution is added dropwise), then carrying out destroy the enzyme treatment, (temperature is 95 DEG C, the time 15
It min), is 4 DEG C in temperature, revolving speed is centrifuged 20 min and takes supernatant under conditions of being 8000 rpm, freeze-drying obtains clam enzyme
Solve protein peptides；
(3) pueraria lobata is clayed into power, sieving (slot size is 60 mesh) obtains pueraria lobata powder, water is added in the pueraria lobata powder
In, the mass volume ratio of pueraria lobata powder and water is 1:180 g/mL, is uniformly mixed, and then heats and carries out decoction processing, at decoction
The temperature of reason is 98 DEG C, and decocting the processing time is 6 min, stands 6 min, is filtered, and revolving obtains pueraria lobata water and mentions after freeze-drying
Object；
(4) hoveniae semoveniae semen is clayed into power, sieving (slot size is 60 mesh) obtains hoveniae semoveniae semen powder；By the hoveniae semoveniae semen powder
It is added to the water, the mass volume ratio of hoveniae semoveniae semen powder and water is 1:180 g/mL, is uniformly mixed, and then heating carries out at decoction
Reason, the temperature for decocting processing is 98 DEG C, and decocting the processing time is 6min, stands 6 min, is filtered, and revolving obtains after freeze-drying
Hoveniae semoveniae semen water extract；
(5) cape jasmine is clayed into power, sieving (slot size is 60 mesh) obtains Cape Jasmine Fruit, water is added in the Cape Jasmine Fruit
In, the mass volume ratio of Cape Jasmine Fruit and water is 1:8 g/mL, is uniformly mixed, is then heated, the temperature of heat treatment
Degree is 80 DEG C, and the time of heat treatment is 3.2 h, is filtered, and revolving obtains cape jasmine water extract after freeze-drying；
(6) by step (2) the clam enzymolysis protein peptide, step (3) the pueraria lobata water extract, step (4) the hoveniae semoveniae semen water
Extract, step (5) the cape jasmine water extract are uniformly mixed, the text with corrigent (selecting orange powder and steviol glycoside herein)
Clam enzymolysis protein peptide, pueraria lobata water extract, hoveniae semoveniae semen water extract and cape jasmine water extract mass ratio be 3:2:2:2, obtain mixture,
The orange powder quality accounts for the 0.27wt% of the mixture quality, and the steviol glycoside quality accounts for the mixture quality
0.1wt% obtains the oxidation resistant pulvis of the natural liver protection then by mixture sieving (slot size is 80 mesh).
Embodiment 3
A kind of preparation method of the oxidation resistant pulvis of natural liver protection, includes the following steps:
(1) clam is taken into meat, removes internal organ, washs and remove the moisture on surface, mashing handles to obtain clam meat gruel (clam meat gruel
Albumen quality score be 14.59%), be beaten processing revolving speed be 20000 r/min, be beaten processing time be 3 min；It will
Clam meat gruel is mixed with water, and the solid-liquid ratio of clam meat gruel and water is 1:9.5 g/mL, is uniformly mixed, is obtained mixed liquor；
(2) protease (neutral proteinase, Neutral protease) is added in step (1) described mixed liquor, protease
Quality is the 0.9wt% of step (1) the clam meat gruel protein content (the albumen quality score of clam meat gruel is 14.59%), is added
Heat carries out enzyme digestion reaction, and the temperature of enzyme digestion reaction is 52 DEG C, and the time of enzyme digestion reaction is 4.2 h, in enzyme digestion reaction, mixed liquor
PH value maintains 6.8(and the maintenance of 0.1 M NaOH solution is added dropwise), then carrying out destroy the enzyme treatment, (temperature is 90 DEG C, the time 20
It min), is 4 DEG C in temperature, revolving speed is centrifuged 20 min and takes supernatant under conditions of being 8000 rpm, freeze-drying obtains clam enzyme
Solve protein peptides；
(3) pueraria lobata is clayed into power, sieving (slot size is 60 mesh) obtains pueraria lobata powder, water is added in the pueraria lobata powder
In, the mass volume ratio of pueraria lobata powder and water is 1:220 g/mL, is uniformly mixed, and then heats and carries out decoction processing, at decoction
The temperature of reason is 95 DEG C, and decocting the processing time is 4 min, stands 4 min, is filtered, and revolving obtains pueraria lobata water and mentions after freeze-drying
Object；
(4) hoveniae semoveniae semen is clayed into power, sieving (slot size is 60 mesh) obtains hoveniae semoveniae semen powder；By the hoveniae semoveniae semen powder
It is added to the water, the mass volume ratio of hoveniae semoveniae semen powder and water is 1:220 g/mL, is uniformly mixed, and then heating carries out at decoction
Reason, the temperature for decocting processing is 95 DEG C, and decocting the processing time is 4 min, stands 4 min, is filtered, revolving, after freeze-drying
To hoveniae semoveniae semen water extract；
(5) cape jasmine is clayed into power, sieving (slot size is 60 mesh) obtains Cape Jasmine Fruit, water is added in the Cape Jasmine Fruit
In, the mass volume ratio of Cape Jasmine Fruit and water is 1:6.5 g/mL, it is uniformly mixed, is then heated, heat treatment
Temperature is 85 DEG C, and the time of heat treatment is 3.5 h, is filtered, and revolving obtains cape jasmine water extract after freeze-drying；
(6) by step (2) the clam enzymolysis protein peptide, step (3) the pueraria lobata water extract, step (4) the hoveniae semoveniae semen water
Extract, step (5) the cape jasmine water extract are uniformly mixed, the text with corrigent (selecting orange powder and steviol glycoside herein)
Clam enzymolysis protein peptide, pueraria lobata water extract, hoveniae semoveniae semen water extract and cape jasmine water extract mass ratio be 3:2.2:1.8:1.8, obtain
Mixture, the orange powder quality account for the 0.2wt% of the mixture quality, and the steviol glycoside quality accounts for the compounding substances
The 0.1wt% of amount obtains the oxidation resistant pulvis of the natural liver protection then by mixture sieving (slot size is 80 mesh).
Implementation result verifying
By 3 parts of oxidation resistant pulvis of natural liver protection made from step (6), step (7) and step (8) in embodiment 1 respectively according to
Solid-liquid ratio 1:30 g/mL is uniformly mixed with water to be brewed, and then carries out sensory evaluation (standard of evaluation is as shown in table 1), as a result such as
Shown in Fig. 7.
Table 1
Sensory evaluation personnel are no sensory handicaps, health, the adult (18 for having certain ability to express, scholar's educational background
People).Drinking water is as the transition agent during sensory evaluation, for cleaning oral cavity, dispelling odor, Yi Mianhou after tasting a sample
Continuous evaluation is influenced by previous sample.For the sample of preparation other than the characteristic to be evaluated, complementary characteristic is all the same, has uniform
Property；Sample number≤8 tested every time, sample temperature are room temperature, using glass capsule as container, are put using the circle method of putting
Sample.Through sensory evaluation (as shown in Figure 7), in Fig. 7,0.20%, 0.15 and 0.10% is the accounting of steviol glycoside quality, 0.20%,
0.15 and 0.10% the oxidation resistant pulvis of natural liver protection made from step (8), step (7) and step (6) is respectively indicated, by Fig. 7
It is found that best compound scheme is that clam enzymolysis protein peptide is mentioned with pueraria lobata water extract, cape jasmine water extract, hoveniae semoveniae semen water in 3 schemes
When amount of substance ratio is 1:1:1:1, it is aided with the orange powder of corrigent mass fraction 0.2%, 0.15% steviol glycoside, after being sufficiently mixed
80 mesh screens are crossed, the scheme (the oxidation resistant pulvis of natural liver protection made from step (7)) of the oxidation resistant pulvis of natural liver protection is obtained,
63.331 μm of average volume particle diameter of compound product made from the program (the oxidation resistant pulvis of natural liver protection), surface area is average
Partial size is 16.981 μm, and specific surface area is 0.353 m2/ g, compound product (the oxidation resistant pulvis of the natural liver protection) size distribution
Figure is as shown in Figure 8.
In conjunction with Fig. 1, Fig. 2, Fig. 3, Fig. 4, Fig. 5, Fig. 6 and Fig. 7, it is possible to find, compared with comparative example, embodiment 1 is resulting multiple
With product (the oxidation resistant pulvis of natural liver protection), bitter taste and fishy smell are decreased obviously, and astringent taste weakens, and therefore, the sweet taste of product is more
Add protrusion, is subjected to degree and increases.The oxidation resistant pulvis effect of natural liver protection made from other embodiments is similar to Example 1,
Bitter taste and fishy smell are decreased obviously, and astringent taste weakens, and sweet taste is more prominent, are subjected to degree height, be can refer to Fig. 7.
The embodiment of the present invention is as it can be seen that entire preparation process flow is simple, each link is up to food-grade requirement, preparation cost
It is lower.
Above embodiments are only preferrred embodiment of the present invention, for explaining only the invention, are not intended to limit the present invention, this
Field technical staff should belong to guarantor of the invention without departing from change made under spirit of the invention, replacement, modification etc.
Protect range.
Claims (10)
1. a kind of preparation method of the oxidation resistant pulvis of natural liver protection, which comprises the steps of:
(1) clam is taken into meat, removes internal organ, washing, mashing handles to obtain clam meat gruel, clam meat gruel is mixed with water, mixes
Uniformly, mixed liquor is obtained；
(2) protease is added in step (1) described mixed liquor, heating carries out enzyme digestion reaction, then carries out destroy the enzyme treatment, is centrifuged
Supernatant is taken, freeze-drying obtains clam enzymolysis protein peptide；
(3) pueraria lobata is clayed into power, is sieved, obtains pueraria lobata powder, the pueraria lobata powder is added to the water, be uniformly mixed, then
Heating carries out decoction processing, stands, and filters, and revolving obtains pueraria lobata water extract after freeze-drying；
(4) hoveniae semoveniae semen is clayed into power, is sieved, obtains hoveniae semoveniae semen powder；The hoveniae semoveniae semen powder is added to the water, mixing is equal
Even, then heating carries out decoction processing, stands, and filters, and revolving obtains hoveniae semoveniae semen water extract after freeze-drying；
(5) cape jasmine is clayed into power, is sieved, obtains Cape Jasmine Fruit, the Cape Jasmine Fruit is added to the water, be uniformly mixed, then
Heated, filtered, revolving obtains cape jasmine water extract after freeze-drying；
(6) by step (2) the clam enzymolysis protein peptide, step (3) the pueraria lobata water extract, step (4) the hoveniae semoveniae semen water
Extract, step (5) the cape jasmine water extract are uniformly mixed with corrigent, obtain mixture, then the mixture is sieved, obtains
To the oxidation resistant pulvis of the natural liver protection.
2. preparation method according to claim 1, which is characterized in that step (1) it is described mashing processing rate be
18000-20000 r/min；The time of mashing processing is 3-5 min；The protein content of the clam meat gruel is greater than 14wt%；Institute
The solid-liquid ratio for stating clam meat gruel and water is 1:8-1:12 g/mL.
3. preparation method according to claim 1, which is characterized in that step (2) protease is neutral proteinase；Step
Suddenly the quality of (2) described protease is the 0.8-1.2wt% of the protein content quality of step (1) described clam meat gruel；Step (2)
The temperature of the enzyme digestion reaction is 53-57 DEG C, and the time of enzyme digestion reaction is 3.5-4 h, and the pH of enzyme digestion reaction is 6.8-7.2.
4. preparation method according to claim 1, which is characterized in that the temperature of step (2) described destroy the enzyme treatment is 90-
100 DEG C, the time of destroy the enzyme treatment is 10-20 min.
5. preparation method according to claim 1, which is characterized in that the slot size of step (3) described sieving is 60 mesh；
The mass volume ratio of the pueraria lobata powder and water is 1:180-1:250 g/mL；The temperature for decocting processing is 95-100 DEG C,
The time for decocting processing is 4-6 min；The time of the standing is 4-6 min.
6. preparation method according to claim 1, which is characterized in that the slot size of step (4) described sieving is 60 mesh；
The mass volume ratio of the hoveniae semoveniae semen powder and water is 1:180-1:250 g/mL；The temperature for decocting processing is 95-100
DEG C, the time for decocting processing is 4-6 min；The time of the standing is 4-6 min.
7. preparation method according to claim 1, which is characterized in that the slot size of step (5) described sieving is 60 mesh；
The mass volume ratio of the Cape Jasmine Fruit and water is 1:6-1:8 g/mL；The temperature of the heat treatment is 80-85 DEG C, described to add
The time of heat treatment is 3-3.5 h.
8. preparation method according to claim 1, which is characterized in that in step (6), according to the mass fraction,
1-3 parts of clam enzymolysis protein peptide；
1-2 parts of pueraria lobata water extract；
1-2 parts of hoveniae semoveniae semen water extract；
1-2 parts of cape jasmine water extract.
9. preparation method according to claim 1, which is characterized in that step (6) corrigent is lemon powder, orange powder
And one or more of steviol glycoside；The quality of the corrigent accounts for the 0-0.37wt% of the mixture quality；The sieving
Slot size is 80 mesh.
10. a kind of oxidation resistant pulvis of natural liver protection as made from claim 1-9 described in any item preparation methods.
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