CN110403022B - Liquid infant milk suitable for 0-12 months old and preparation method thereof - Google Patents
Liquid infant milk suitable for 0-12 months old and preparation method thereof Download PDFInfo
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- CN110403022B CN110403022B CN201810400000.9A CN201810400000A CN110403022B CN 110403022 B CN110403022 B CN 110403022B CN 201810400000 A CN201810400000 A CN 201810400000A CN 110403022 B CN110403022 B CN 110403022B
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C21/00—Whey; Whey preparations
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C21/00—Whey; Whey preparations
- A23C21/04—Whey; Whey preparations containing non-milk components as source of fats or proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C21/00—Whey; Whey preparations
- A23C21/06—Mixtures of whey with milk products or milk components
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C21/00—Whey; Whey preparations
- A23C21/08—Whey; Whey preparations containing other organic additives, e.g. vegetable or animal products
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention provides liquid infant milk suitable for 0-12 months old and a preparation method thereof, wherein the liquid infant milk also contains polypeptide and carrageenan on the premise of meeting the national standard of infant formula, and the content of the polypeptide is more than 180.0mg per 100ml of liquid milk; the carrageenan content is 0.005-0.03 g/100ml liquid milk.
Description
Technical Field
The invention relates to liquid infant milk and a preparation method thereof, in particular to liquid infant milk suitable for infants of 0-12 months old and a preparation method thereof, belonging to the technical field of infant milk.
Background
The infant stage has significance, the growth and development of the infant in the stage are very fast, the requirement for nutrition is very high, the infant is not only required to be fed with food with balanced and comprehensive nutrition, but also is required to be digestible, because the digestion and the metabolic system development of the infant stage are not very perfect, and particularly the food which is digestible for infants which are lack of breast feeding due to working requirements, physical conditions and the like is not a best choice. However, at present, the types of milk substitute foods in the market in China are few, the types of nutrition supplement foods suitable for infants to eat are limited, infant milk powder and rice flour are mainly used in the infant stage, and the products are mainly in solid forms; it is difficult to ensure daily normal nutrient intake due to limitations in life and eating habits, physical conditions, and the like. Therefore, beyond the limitations of existing product formats, the development of high quality infant formulas is of great social and economic importance.
The best food for infants is breast milk, which contains nutrients necessary for infant growth and components for enhancing infant immunity, and although breast feeding is advocated worldwide, more and more people are aware of the importance of breast feeding, the breast feeding rate in China is gradually decreasing for various reasons, especially the breast feeding rate in urban population is decreasing faster. Therefore, the milk substitute product which has comprehensive nutrition, higher quality and convenient brewing is definitely not two choices for the part of people with relatively higher income and relatively faster life rhythm.
Milk, in addition to breast milk, is widely available and has a relatively close composition to breast milk, and is a major milk substitute for infants. However, bovine milk proteins are compositionally different from human milk proteins, and casein in bovine milk tends to form larger clots in the infant's stomach, resulting in dyspepsia in the infant; beta-lactoglobulin is liable to cause allergic reactions in infants, etc. Accordingly, there is a need for appropriate modification of cow's milk to more closely approximate the nutritional composition of breast milk to produce a formula that is suitable for both digestion and nutritional needs of infants. Liquid infant milk is popular in many countries abroad, but the infant formula in China mainly uses solid infant milk powder, and compared with the infant milk powder, the liquid infant milk has many advantages such as no need of brewing, constant concentration, convenient feeding, safety and sanitation and the like. The development of infant formulas in the liquid state has become a trend, and the liquid infant formulas are still blank in the domestic market at present, so that the development prospect is wide, and the liquid infant formulas are expected to have a market share of 3-5 hundred million yuan by 2020.
For liquid infant milk, there is no great difference in the formulation, and the technical difficulty in producing the product is in the sterilization process and stability of the product. CN101313702a discloses a liquid milk for infants between 6 and 12 months and a preparation method using spray type direct sterilization, which uses UHT steam spray type direct sterilization technology to sterilize the product. CN102630760a discloses an infant formula concentrated milk and a preparation method thereof, which adopts a UHT mode to sterilize the product. CN102511557a discloses a liquid infant milk suitable for birth to 6 months and a production method thereof, wherein a heat-sensitive water-soluble material is treated by adopting a combination mode of membrane filtration and low-heat sterilization.
From the above prior art, it can be seen that each dairy enterprise adopts different sterilization techniques to treat the products, mainly because the products can generate protein denaturation, precipitation and stability problems during high-temperature sterilization, such as the problem that microorganisms are uncontrollable when an improved low-temperature treatment rule is adopted, a large amount of emulsion stabilizers are almost used for the products to compensate for the defect of thermally stable development of the products, which is very unfavorable for cleaning the product labels.
In view of the above, there is a need in the art to provide liquid infant milk that has good heat stability, good cleanliness, easy digestion, and less susceptibility to allergy, and a method of preparing the same.
Disclosure of Invention
In view of the actual needs, an object of the present invention is to provide a liquid infant milk suitable for infants between 0 and 12 months, so as to obtain a liquid infant milk with good stability by a high-temperature sterilization method with the least amount of added stabilizer.
Another object of the present invention is to provide a method for preparing the liquid infant milk described above.
To achieve the above object, the present invention provides a liquid infant milk suitable for 0 to 12 months old, comprising, based on the liquid infant milk corresponding to each 100KJ calories: 0.45 to 0.70g of protein, 1.05 to 1.40g of fat, 2.2 to 3.3g of carbohydrate, 0.07 to 0.33g of linoleic acid, more than or equal to 12mg of linseed oil, 14 to 43 mu g of vitamin A, 0.25 to 0.60 mu g of vitamin D, 0.12 to 0.20mg of vitamin E, 1.0 to 6.5 mu g of vitamin K1, 14 to 72 mu g of vitamin B1, 19 to 119 mu g of vitamin B2, 8.5 to 45.0 mu g of vitamin B6, 0.025 to 0.360 mu g of vitamin B12, 70 to 360 mu g of nicotinic acid, 2.5 to 12.0 mu g of folic acid, 96 to 478 mu g of pantothenic acid, 2.5 to 17.0mg of vitamin C, 0.4 to 2.4 mu g of biotin, 5 to 14mg of sodium, 14 to 43mg of potassium, 8.5 to 29.0 mu g of copper, 1.2 to 3.6mg of magnesium, 0.36mg of zinc, 0.24 to 12mg of zinc, 0.24 mg to 12 mu g of zinc, 0.36mg of zinc, 0.24 mg to 0.36mg of zinc, 0.24 mg of zinc, and less than or equal to 0.6 mg of zinc, 0.24 mg to 0.36mg of zinc, and less than or equal to 0.6 mg of zinc;
each 100mL of liquid infant milk contains 250-295 KJ of heat, and the liquid infant milk contains polypeptide and carrageenan, wherein the content of the polypeptide is more than 180.0mg, preferably 200.0-450.0 mg, of the polypeptide in each 100mL of liquid infant milk; the carrageenan content is 0.005-0.03 g/100ml liquid milk.
On the premise of meeting the national standard (GB 10765-2010) of infant formula, the polypeptide and the carrageenan are added to the liquid infant milk innovatively, and the polypeptide and the carrageenan act together, so that the heat stability of the liquid infant milk is obviously improved, the liquid infant milk can be sterilized at high temperature without affecting the stability of a final product, the sediment amount and the fat floating amount of the final product are obviously reduced, and the final product also has good color. Compared with the existing liquid infant milk, the emulsion stabilizing system is greatly simplified, so that the product label is cleaner. Moreover, compared with the existing liquid infant milk, the infant milk disclosed by the invention has the advantages that the digestion and absorption capacity of an infant can be improved and the allergy is reduced due to the fact that the infant milk contains the polypeptide. The infant formula provided by the invention has the advantages of balanced nutrition, stable quality, portability, no need of brewing or dilution and convenience in drinking.
The contents or proportions of the invention are all weights or weight proportions unless otherwise indicated.
In the invention, the polypeptide content in the liquid infant milk is measured by a trichloroacetic acid method. As mentioned above, it contains more than 180.0mg polypeptide per 100ml liquid milk, preferably 200.0-450.0 mg, e.g. 226.0mg/100ml, 285.0mg/100ml.
As described above, the carrageenan is used in an amount of 0.005-0.03 g/100ml of the liquid infant milk. For example, 0.010g/100ml, 0.015g/100ml, 0.020g/100ml, 0.025g/100ml, 0.030g/100ml, etc.
The milk peptide of the present invention may be formed into a polypeptide in the above content range in the production method, for example, by hydrolyzing a protein-containing raw material such as raw milk with a protease in the production method, or may be directly added to liquid milk, for example, hydrolyzed desalted whey powder. In some embodiments of the present invention, preferably, the liquid infant milk has a polypeptide content of 180.0-450.0 mg per 100ml by adding a milk material in which part or all of the protein is enzymatically hydrolyzed by protease. More preferably, the milk material after enzymolysis by protease comprises one or more of raw cow milk hydrolysate, hydrolyzed desalted whey powder and hydrolyzed concentrated whey protein powder. In some embodiments, the raw cow milk hydrolysate has a polypeptide content of 3.0-9.0 mg/mL. In some embodiments, the hydrolyzed desalted whey powder has a polypeptide content of 12.0 to 36.0mg/g. In some embodiments, the polypeptide content of the hydrolyzed concentrated whey protein powder is 80.0-392.0 mg/g, typically 80.0-240.0 mg/g.
In some embodiments of the present invention, preferably, the raw material composition per 1000Kg of said liquid infant milk comprises the following components: 128.5 to 320Kg of raw milk hydrolysate, 20 to 90Kg of hydrolyzed desalted whey powder, 0 to 18Kg of hydrolyzed concentrated whey protein powder, 8.9 to 15.5Kg of anhydrous cream, 10.0 to 15.4Kg of vegetable oil, 0 to 25.4Kg of lactose, 0.32 to 0.6Kg of compound vitamin, 0.64 to 0.96Kg of compound mineral, 0.075 to 0.115Kg of choline, 0.065 to 0.095Kg of taurine, 0.05 to 0.3Kg of carrageenan, 0.05 to 0.25Kg of citric acid and the balance of supplementing water to 1000Kg.
Preferably, the raw milk hydrolysate in the liquid infant milk is formed from milk. More preferably, the polypeptide content in the raw milk hydrolysate is 3.0-9.0 mg/mL.
Preferably, the hydrolyzed desalted whey powder in the liquid infant milk described above is formed from desalted whey powder. More preferably, the polypeptide content in the desalted whey powder is 12.0-36.0 mg/g.
Preferably, the hydrolyzed concentrated whey protein powder in the liquid infant milk is formed from concentrated whey protein powder. More preferably, the polypeptide content in the hydrolyzed concentrated whey protein powder is 80.0-240.0 mg/g.
The raw material composition of the liquid infant milk is based on, but not limited to, the formula provided by the invention, and nutrients beneficial to the growth and development of infants can be added on the basis of the formula provided by the invention. Such as MFGM (milk fat globule membrane), DHA, lactoferrin, etc.
The raw material composition of the liquid infant milk is based on, but not limited to, the formula provided by the invention, and other food raw materials (other protein raw materials or unsaturated fatty acid, phospholipid and the like) and food additives such as emulsifying agents (glycerol monostearate, glycerol citrate and the like, the dosage of which is referred to national standard), stabilizing agents (locust bean gum, guar gum and the like, and the dosage of which is referred to national standard) and the like can be added on the basis of the formula provided by the invention.
In another aspect, the present invention provides a method for preparing the liquid infant milk, comprising the steps of:
(1) Preparing or preparing materials for providing polypeptide and other raw materials, mixing, and supplementing total weight with water;
(2) Homogenizing the mixed material obtained in the step (1); preferably, homogenizing is carried out under the conditions that the primary homogenizing pressure is 15-20MPa and the secondary homogenizing pressure is 3-5 MPa;
(3) Sterilizing the homogenized material in the step (2), preferably performing sterilization treatment by UHT, and more preferably performing sterilization treatment at 130-140 ℃ for 2-5 s;
(4) And cooling the sterilized material to obtain the liquid infant milk.
In some embodiments, step (1) directly prepares the polypeptide-providing material, comprising the steps of:
(1.1) subjecting part or all of the milk material used to provide the protein in the liquid infant milk to a heat treatment to denature the protein therein, then cooling the solution to a temperature suitable for proteolytic hydrolysis, and adding water before or after cooling to form a system to be hydrolysed (preferably, water is added to the milk material to provide the protein in the liquid infant milk before heat treatment);
(1.2) adding protease to the cooled solution obtained in the step (1.1) for hydrolysis, and thermally inactivating the protease after the hydrolysis to obtain the material for providing the polypeptide.
In the above method of preparing the polypeptide-providing material, preferably, the milk material for providing protein in the liquid infant milk comprises one or more of raw cow milk, desalted whey powder, concentrated whey protein or other protein-containing milk material.
In the above method for producing a polypeptide-providing material, preferably, the protein concentration in the system to be hydrolyzed is 3.0% to 20.0%.
In the above method for preparing the polypeptide-providing material, preferably, the mass ratio of water in the system to be hydrolyzed is about 80% to 90% before adding the protease.
In the above method for preparing the polypeptide-providing material, preferably, in the step (2), the protease is a compound protease prepared by mixing one or more proteases meeting the requirements of GB2760 on proteases in food according to different proportions. More preferably, the protease is a complex protease comprising 3 to 8 weight percent: 1 and neutral proteases F (Validase FP Concentrate) and G (Protease GBW). The addition amount of the protease is 0.4-0.9% of the mass of the protein substrate.
In the above method for preparing the polypeptide-providing material, preferably, the protease is added for hydrolysis at an optimum temperature and pH for a period of time such that the content of the polypeptide produced by the hydrolysis reaches the content of the polypeptide in the final product liquid infant milk, preferably for a period of time of 0.5 to 5.0 hours.
In the step (3) of the invention, the conventional sterilization mode or the combination thereof in the prior art can be adopted, so long as the sterilization effect is achieved, and UHT sterilization can be adopted in consideration of the simplification of the process due to the heat stability of the formula.
As described above, in order to allow the liquid infant milk of the present invention to contain a predetermined amount of polypeptide, a part or all of the protein material in the composition of the raw material of the product may be hydrolyzed, or the product may be produced by directly adding hydrolyzed protein (hydrolyzed whey protein, hydrolyzed casein, etc.) as a raw material.
Preferably, the preparation method of the liquid infant milk comprises the following steps:
(1.1) mixing part or all of the milk material used to provide the protein in the liquid infant milk with water to prepare a solution, and performing a heat treatment to denature the protein therein, and then cooling the solution to a temperature suitable for proteolytic hydrolysis;
(1.2) adding protease into the cooled solution obtained in the step (1.1), hydrolyzing under the condition of optimal temperature and pH until the content of the polypeptide in the product meets the requirement, and thermally inactivating the protease after hydrolysis to obtain a material for providing the polypeptide;
(1.3) mixing the polypeptide-providing material obtained in step (1.2) with the rest of the raw materials, and supplementing the total weight with water;
(2) Homogenizing the mixed material obtained in the step (1.3); homogenizing under the conditions that the primary homogenizing pressure is 15-20MPa and the secondary homogenizing pressure is 3-5 MPa;
(3) Sterilizing the homogenized material in the step (2), and performing UHT sterilization under the condition of processing for 2-5s at 130-140 ℃;
(4) And cooling the sterilized material to obtain the liquid infant milk.
The liquid infant milk obtained by the invention can be aseptically filled according to the conventional technology. The raw materials used in the invention are all food-grade and are commercially available. The water used in the invention is water which is conventional in the field, and deionized water is more suitable.
In summary, the invention provides liquid infant milk suitable for infants of 0-12 months old and a preparation method thereof, which has the following beneficial effects:
(1) The invention can hydrolyze protein to release polypeptide by using biological enzymolysis technology and take the polypeptide as one of final product stabilizing systems, and the polypeptide and carrageenan act together to achieve better stabilizing effect, simplify the composition of the product stabilizing system and clean the product label;
(2) The invention also overcomes the problem of protein denaturation and precipitation in the preparation process of the infant formula, improves the thermal stability of the product, ensures that the infant formula can adopt a high-temperature sterilization mode, and simplifies the production process;
(3) The invention relieves the phenomenon of dyspepsia of infants caused by more macromolecular proteins in infant formula milk;
(4) The invention can relieve the allergy of infants caused by eating macromolecular milk proteins (mainly beta-lactoglobulin).
Drawings
Fig. 1 is a schematic flow chart of the preparation of liquid infant milk according to example 1 of the present invention.
Detailed Description
In order to more clearly understand the technical features, objects and advantages of the present invention, the following detailed description will be made with reference to specific embodiments thereof, and it should be understood that these examples are only for illustrating the present invention and are not intended to limit the scope of the present invention. In the examples, each of the starting reagent materials is commercially available, and the experimental methods without specifying the specific conditions are conventional methods and conventional conditions well known in the art, or according to the conditions recommended by the instrument manufacturer.
The polypeptides in the following examples were determined by trichloroacetic acid method, which comprises the following steps:
(1) And (3) standard curve preparation: taking glutathione standard solution (0.8 mg/mL), sequentially sucking the solutions 0, 1, 2, 3, 4 and 5mL, placing the solutions in 6 test tubes, respectively adding 3mL of biuret reagent, fixing the volume to 10mL by distilled water, performing color development for 5min in a water bath at 60 ℃, continuously oscillating during the water bath, cooling to room temperature (25-35 ℃), and measuring OD value at 310nm (taking the 1 st tube as blank control). A standard curve was prepared with peptide concentration on the abscissa X (mg/mL) and OD value on the ordinate Y.
(2) 2.5mL of the sample solution was taken, 2.5mL of 10% (w/v) aqueous trichloroacetic acid (TCA) was added, mixed well on a vortex mixer, left to stand for 20min, and then centrifuged at 3500r/min for 10min.
(3) 1.0mL of the above solution was placed in a test tube, 3.0mL of biuret reagent (sample solution: biuret reagent=3:2, v/v) was added, and the mixture was uniformly mixed on a vortex mixer, developed in a water bath at 60℃for 5min, and centrifuged at 2000r/min for 10min.
(4) And taking supernatant to measure an OD value at 310nm, and comparing the OD value with a standard curve to obtain the concentration C (mg/m L) of the polypeptide in the sample solution, so that the content of the polypeptide in the sample can be determined.
Example 1
The invention provides an infant formula and a preparation method thereof, wherein the dry matter content of the infant formula is 12.5wt.%, and each 1000Kg of the infant formula comprises the following components: 255.75Kg of raw milk hydrolysate, 61.50Kg of hydrolyzed desalted whey powder, 14.5Kg of anhydrous cream, 10.0Kg of vegetable oil, 9.5Kg of lactose, 0.32Kg of compound vitamin, 0.64Kg of compound mineral matter, 0.075Kg of choline, 0.065Kg of taurine, 0.15Kg of carrageenan, 0.05Kg of citric acid and the balance of water to 1000Kg.
As shown in the flow chart of fig. 1, the preparation method of the infant formula comprises the following steps:
(1) After 255.75Kg of fresh raw milk is checked, filtered and purified, the protein is denatured by heat treatment for 10min at 80 ℃, and the solution is cooled to 55 ℃;
(2) Adding protease (neutral protease F (Validase FP Concentrate, available from Dissman trade (Shanghai) Co.) and neutral protease G to the above solutionProtease GBW, purchased from Dissman trade (Shanghai) Co., ltd.) according to a ratio of 5:1), and the addition amount of the compound Protease is 0.4% of the content of the substrate protein, hydrolyzing for 1.0h under stirring at 55deg.C, and heating the solution at 80deg.C for 10min to inactivate the Protease to obtain raw milk hydrolysate; the content of polypeptide in the raw milk hydrolysate is 4.5mg/mL measured by trichloroacetic acid method.
(3) Mixing the hydrolyzed solution with 61.50Kg hydrolyzed desalted whey powder (34.8 mg/g polypeptide content in hydrolyzed desalted whey powder), 0.15Kg carrageenan, 14.5Kg anhydrous butter and 10.0Kg vegetable oil, cooling the solution to 60deg.C, adding 0.32Kg compound vitamin, 0.64Kg compound mineral, 0.075Kg choline, 0.065Kg taurine and 0.05Kg citric acid, and adding water to 1000Kg;
(4) Homogenizing the mixed feed liquid under the conditions that the primary homogenizing pressure is 15-20MPa and the secondary homogenizing pressure is 3-5 MPa;
(5) Treating the homogenized feed liquid at 130-140 ℃ for 2-5s to perform UHT sterilization;
(6) Cooling the UHT sterilized feed liquid, aseptically filling, and checking to obtain the final product.
The content of the polypeptide in the finished product is 3.29g/kg by adopting a trichloroacetic acid method.
Comparative example 1
An infant formula was prepared as comparative example 1 in the same manner as the product formulation and preparation method of example 1 except that raw milk was not subjected to enzymatic treatment and desalted whey powder was replaced with desalted whey powder.
The preparation method of the comparative example 1 is as follows, and the raw materials and the process meet the national relevant standards.
The infant formula contained the following components per 1000Kg of infant formula with a dry matter content of 12.5 wt.%: 255.75Kg of raw milk, 61.50Kg of desalted whey powder, 14.5Kg of anhydrous cream, 10.0Kg of vegetable oil, 9.5Kg of lactose, 0.32Kg of compound vitamin, 0.64Kg of compound mineral matter, 0.075Kg of choline, 0.065Kg of taurine, 0.15Kg of carrageenan, 0.05Kg of citric acid and the balance of water to 1000Kg.
(1) Firstly, dissolving desalted whey powder with water accounting for 30% of the total amount of ingredients at about 80 ℃, and then respectively mixing 255.75Kg raw milk, 0.15Kg carrageenan, 14.5Kg anhydrous cream and 10.0Kg vegetable oil which are subjected to acceptance inspection, filtration and purification with the dissolved materials in sequence;
(2) After cooling to 60 ℃, adding 9.5Kg lactose, 0.32Kg compound vitamin, 0.64Kg compound mineral, 0.075Kg choline, 0.065Kg taurine and 0.05Kg citric acid into the material (1) and supplementing 1000Kg for dissolution;
(3) Homogenizing the mixed feed liquid under the conditions that the primary homogenizing pressure is 15-20MPa and the secondary homogenizing pressure is 3-5 MPa;
(4) Treating the homogenized feed liquid at 130-140 ℃ for 2-5s to perform UHT sterilization;
(5) Cooling the UHT sterilized feed liquid, aseptically filling, and checking to obtain the final product.
The content of the polypeptide in the finished product is 1.69g/kg by adopting a trichloroacetic acid method.
Comparative example 2
An infant formula was prepared as comparative example 2 in accordance with the product formulation and preparation method of example 1, except that the stabilizer carrageenan was not added.
The preparation method of comparative example 2 is as follows, and the raw materials and the process meet the national relevant standards.
The infant formula contained the following components per 1000Kg of infant formula with a dry matter content of 12.5 wt.%: 255.75Kg of raw milk hydrolysate, 61.50Kg of hydrolyzed desalted whey powder, 14.5Kg of anhydrous cream, 10.0Kg of vegetable oil, 9.5Kg of lactose, 0.32Kg of compound vitamin, 0.64Kg of compound mineral matter, 0.075Kg of choline, 0.065Kg of taurine, 0.05Kg of citric acid and the balance of water to 1000Kg.
Wherein the preparation method of the infant formula comprises the following steps:
(1) Carrying out heat treatment on the raw milk subjected to acceptance, filtration and purification at 80 ℃ for 10min to denature protein, and then cooling the solution to 55 ℃;
(2) Adding protease (neutral protease F (Validase FP Concentrate, available from Dissman trade (Shanghai) Co.) and neutral protease G to the above solutionProtease GBW, purchased from Dissman trade (Shanghai) Co., ltd.) according to a ratio of 5:1), and the addition amount of the compound Protease is 0.4% of the content of the substrate protein, hydrolyzing for 1.0h under stirring at 55deg.C, and heating the solution at 80deg.C for 10min to inactivate the Protease to obtain raw milk hydrolysate; the content of polypeptide in the raw milk hydrolysate is 4.5mg/mL measured by trichloroacetic acid method.
(3) Mixing the hydrolyzed solution with 61.50Kg hydrolyzed desalted whey powder (34.8 mg/g polypeptide content in hydrolyzed desalted whey powder), 14.5Kg anhydrous butter and 10.0Kg vegetable oil, cooling to 60deg.C, adding 0.32Kg compound vitamin, 0.64Kg compound mineral, 0.075Kg choline, 0.065Kg taurine, and 0.05Kg citric acid, mixing, and supplementing 1000Kg with water;
(4) Homogenizing the mixed feed liquid under the conditions that the primary homogenizing pressure is 15-20MPa and the secondary homogenizing pressure is 3-5 MPa;
(5) Treating the homogenized feed liquid at 130-140 ℃ for 2-5s to perform UHT sterilization;
(6) Cooling the UHT sterilized feed liquid, aseptically filling, and checking to obtain the final product.
The content of the polypeptide in the finished product is 3.29g/kg by adopting a trichloroacetic acid method.
Effect example 1
Taking the infant formula prepared in the embodiment 1 of the invention as an example, the infant formula is compared with the nutrition indexes of comparative examples 1 and 2 according to the national food safety standard infant formula GB10765-2010 as a reference, and the comparison results are shown in the table 1. The products obtained in example 1, comparative example 1 and comparative example 2 were tested for each index according to the method in food safety national standard infant formula GB10765-2010, the results obtained are shown in Table 1 below, and the specifications of each index by GB10765-2010 are also shown in Table 1:
table 1 evaluation of nutritional metrics
From table 1, it can be seen that the infant formulas prepared in example 1 and comparative examples 1 and 2 have almost no difference in nutrition index, and all meet the requirements specified in national food safety standard infant formulas GB10765-2010, and are suitable for infants of 0-12 months of age to eat directly.
Effect example 2
The stability evaluation of the infant formula provided by the invention comprises the following steps:
the products of the invention of example 1 and comparative example 2 were used as test samples, and the color, precipitation amount and fat floating amount of the infant formula 3 were measured at room temperature (about 10-25 ℃) for 7 days, 30 days and 60 days, respectively.
The amount of sediment was measured by centrifugation: the sample was placed in a centrifuge tube, centrifuged at 3000 rpm for 15 minutes, and the supernatant was sufficiently removed and the sediment was weighed.
Fat lift was measured using a ruler: the product is aseptically filled in a colorimetric tube of 100mL and placed still. The thickness of the white fat layer on the surface of the milk was measured with a ruler with an accuracy of 0.5mm through the wall of the glass bottle in a stationary state, and the eyes were seen in a flat view during reading.
The product color evaluation method comprises the following steps: please 5 food sensory evaluation panelists evaluate the color of the above 3 groups of infant formulas, and the final color evaluation results were averaged to score 5 persons, and the comparison results are shown in table 3. The color evaluation was performed by a 5-point scoring test, and the scale types and conversion score values are shown in Table 2 below:
TABLE 2
| Preference for | Very good | Good (good) | In general | Is bad | Very bad is |
| Score of | 5 | 4 | 3 | 2 | 1 |
Table 3 stability evaluation
As can be seen from the stability test data, the infant formula of the invention basically has no change after being placed for 7 days at normal temperature, the precipitation amount generated in the examples is the least after being placed for 30 days, but compared with the examples, the precipitation amounts of the products of comparative examples 1 and 2 are obviously different, and the precipitation amount is larger. When only proper hydrolysis is carried out on the protein or only carrageenan is added, the stability of the system can not achieve the effect of cooperation.
The stability experimental data prove that the infant formula and the preparation method thereof can obtain shelf life and shelf life suitable for transportation and sale.
From the experimental data, the infant formula disclosed by the invention is comprehensive in nutrition and excellent in stability, and more importantly, the infant formula product with refined protein and high thermal stability can be obtained by adopting the preparation method disclosed by the invention, and the product is beneficial to digestion and comprehensive absorption of the infant nutrition.
Example 2
The invention provides an infant formula and a preparation method thereof, wherein the dry matter content of the infant formula is 12.5wt.%, and each 1000Kg of the infant formula comprises the following components: 255.75Kg of raw milk hydrolysate, 61.50Kg of hydrolyzed desalted whey powder, 14.5Kg of anhydrous cream, 10.0Kg of vegetable oil, 9.5Kg of lactose, 0.32Kg of compound vitamin, 0.64Kg of compound mineral matter, 0.075Kg of choline, 0.065Kg of taurine, 0.20Kg of carrageenan, 0.05Kg of citric acid and the balance of water to 1000Kg.
As shown in the flow chart of fig. 1, the preparation method of the infant formula comprises the following steps:
(1) After 255.75Kg of fresh raw milk is checked, filtered and purified, the protein is denatured by heat treatment for 10min at 80 ℃, and the solution is cooled to 55 ℃;
(2) Adding protease Amano2SD (purchased from Tianye enzyme products Co., ltd.) into the solution for enzymolysis, wherein the addition amount of the protease is 0.3% of the substrate protein content, hydrolyzing for 1.0h under stirring at 55deg.C, and heating the solution at 80deg.C for 10min to inactivate the protease to obtain raw milk hydrolysate; the content of polypeptide in the raw milk hydrolysate is 4.0mg/mL measured by trichloroacetic acid method.
(3) Mixing the hydrolyzed solution with 61.50Kg hydrolyzed desalted whey powder (34.8 mg/g polypeptide content in hydrolyzed desalted whey powder), 0.15Kg carrageenan, 14.5Kg anhydrous butter and 10.0Kg vegetable oil, cooling the solution to 60deg.C, adding 0.32Kg compound vitamin, 0.64Kg compound mineral, 0.075Kg choline, 0.065Kg taurine and 0.05Kg citric acid, and adding water to 1000Kg;
(4) Homogenizing the mixed feed liquid under the conditions that the primary homogenizing pressure is 15-20MPa and the secondary homogenizing pressure is 3-5 MPa;
(5) Treating the homogenized feed liquid at 130-140 ℃ for 2-5s to perform UHT sterilization;
(6) Cooling the UHT sterilized feed liquid, aseptically filling, and checking to obtain the final product.
The content of polypeptide in the finished product is 3.16g/kg by adopting a trichloroacetic acid method.
The nutrition index and stability of the finished product prepared by the method meet the requirements.
Example 3
The invention provides an infant formula and a preparation method thereof, wherein the dry matter content of the infant formula is 12.5wt.%, and each 1000Kg of the infant formula comprises the following components: 255.75Kg of raw milk hydrolysate, 61.50Kg of hydrolyzed desalted whey powder, 14.5Kg of anhydrous cream, 10.0Kg of vegetable oil, 9.5Kg of lactose, 0.32Kg of compound vitamin, 0.64Kg of compound mineral matter, 0.075Kg of choline, 0.065Kg of taurine, 0.10Kg of carrageenan, 0.05Kg of citric acid and the balance of water to 1000Kg.
As shown in the flow chart of fig. 1, the preparation method of the infant formula comprises the following steps:
(1) After 255.75Kg of fresh raw milk is checked, filtered and purified, the protein is denatured by heat treatment for 10min at 80 ℃, and the solution is cooled to 50 ℃;
(2) Adding protease Amano C100 (purchased from Tianye enzyme products Co., ltd.) into the solution for enzymolysis, wherein the addition amount of protease is 0.4% of the substrate protein content, hydrolyzing for 1.0h under stirring at 50deg.C, and heating the solution at 80deg.C for 10min to inactivate protease to obtain raw milk hydrolysate; the content of polypeptide in the raw milk hydrolysate is 3.0mg/mL measured by trichloroacetic acid method.
(3) Mixing the hydrolyzed solution with 61.50Kg hydrolyzed desalted whey powder (34.8 mg/g polypeptide content in hydrolyzed desalted whey powder), 0.15Kg carrageenan, 14.5Kg anhydrous butter and 10.0Kg vegetable oil, cooling the solution to 60deg.C, adding 0.32Kg compound vitamin, 0.64Kg compound mineral, 0.075Kg choline, 0.065Kg taurine and 0.05Kg citric acid, and adding water to 1000Kg;
(4) Homogenizing the mixed feed liquid under the conditions that the primary homogenizing pressure is 15-20MPa and the secondary homogenizing pressure is 3-5 MPa;
(5) Treating the homogenized feed liquid at 130-140 ℃ for 2-5s to perform UHT sterilization;
(6) Cooling the UHT sterilized feed liquid, aseptically filling, and checking to obtain the final product.
The content of the polypeptide in the finished product is detected to be 2.90g/kg by adopting a trichloroacetic acid method.
The nutrition index and stability of the finished product prepared by the method meet the requirements.
Example 4
The invention provides an infant formula and a preparation method thereof, wherein the dry matter content of the infant formula is 12.5wt.%, and each 1000Kg of the infant formula comprises the following components: 255.75Kg of raw milk hydrolysate, 61.50Kg of hydrolyzed desalted whey powder, 14.5Kg of anhydrous cream, 10.0Kg of vegetable oil, 9.5Kg of lactose, 0.32Kg of compound vitamin, 0.64Kg of compound mineral matter, 0.075Kg of choline, 0.065Kg of taurine, 0.25Kg of carrageenan, 0.05Kg of citric acid and the balance of water to 1000Kg.
As shown in the flow chart of fig. 1, the preparation method of the infant formula comprises the following steps:
(1) After 255.75Kg of fresh raw milk is checked, filtered and purified, the protein is denatured by heat treatment for 10min at 80 ℃, and the solution is cooled to 50 ℃;
(2) Adding neutral protease Neutrase (purchased from NoveXin enzyme preparation Co., ltd.) into the solution for enzymolysis, wherein the addition amount of protease is 0.8% of the substrate protein content, hydrolyzing for 1.0h under stirring at 50deg.C, and heating the solution at 80deg.C for 10min to inactivate protease to obtain raw milk hydrolysate; the content of polypeptide in the raw milk hydrolysate is 8.0mg/mL measured by trichloroacetic acid method.
(3) Mixing the hydrolyzed solution with 61.50Kg hydrolyzed desalted whey powder (34.8 mg/g polypeptide content in hydrolyzed desalted whey powder), 0.15Kg carrageenan, 14.5Kg anhydrous butter and 10.0Kg vegetable oil, cooling the solution to 60deg.C, adding 0.32Kg compound vitamin, 0.64Kg compound mineral, 0.075Kg choline, 0.065Kg taurine and 0.05Kg citric acid, and adding water to 1000Kg;
(4) Homogenizing the mixed feed liquid under the conditions that the primary homogenizing pressure is 15-20MPa and the secondary homogenizing pressure is 3-5 MPa;
(5) Treating the homogenized feed liquid at 130-140 ℃ for 2-5s to perform UHT sterilization;
(6) Cooling the UHT sterilized feed liquid, aseptically filling, and checking to obtain the final product.
The content of the polypeptide in the finished product is detected to be 4.19g/kg by adopting a trichloroacetic acid method.
The nutrition index and stability of the finished product prepared by the method meet the requirements.
The last explanation is: the above embodiments are only for illustrating the implementation procedure and features of the present invention, and not for limiting the technical solution of the present invention, and although the present invention has been described in detail with reference to the above embodiments, it should be understood by those of ordinary skill in the art that: modifications and equivalents may be made thereto without departing from the spirit and scope of the invention, and any modifications and equivalents are intended to be included within the scope of the present invention.
Claims (15)
1. A liquid infant milk suitable for 0-12 months old, comprising, based on the liquid infant milk corresponding to each 100KJ calories: 0.45-0.70 g of protein, 1.05-1.40 g of fat, 2.2-3.3 g of carbohydrate, 0.07-0.33 g of linoleic acid, more than or equal to 12. 12mg linseed oil, 14-43 g of vitamin A, 0.25-0.60 g of vitamin D, 0.12-0.20 mg of vitamin E, 1.0-6.5 g of vitamin K1, 14-72 g of vitamin B1, 19-119 g of vitamin B2, 8.5-45.0 g of vitamin B6, 0.025-0.360 g of vitamin B12, 70-360 g of nicotinic acid, 2.5-12.0 g of folic acid, 96-478 g of pantothenic acid, 2.5-17.0 mg of vitamin C, 0.4-2.4 g of biotin, 5-14 mg of sodium, 14-43 mg of potassium, 8.5-29.0 g of copper, 1.2-3.6 mg of magnesium, 14-72 mg of vitamin B1, 19-119 g of vitamin B2, 8.5-45.0 mg of zinc, 0.025-0.360 g of calcium chloride, 70-360 g of nicotinic acid, 2.5-12.0 mg of folic acid, 96-478 mg of pantothenic acid, 2.5-0 mg of vitamin C, 0.0.6 mg of zinc and 60 mg of zinc chloride;
each 100mL of liquid infant milk contains 250-295 KJ of heat, and the liquid infant milk contains polypeptide and carrageenan, wherein the content of the polypeptide is more than 180.0 and mg per 100mL of liquid milk; the carrageenan content is 0.005-0.03 g in every 100ml of liquid milk;
wherein, in the preparation method, protease is adopted to hydrolyze the raw material containing protein, or the prepared raw material containing polypeptide is directly added into liquid milk to form the polypeptide with the content in the range.
2. The liquid infant milk according to claim 1, wherein the polypeptide is contained in an amount of 200.0-450.0 mg per 100ml of liquid milk; the carrageenan content is 0.01-0.025/g/100 ml liquid milk.
3. The liquid infant milk according to claim 1, wherein the liquid infant milk has a polypeptide content of 180.0 or more mg per 100ml by adding a milk material in which part or all of the protein is enzymatically hydrolyzed by protease.
4. A liquid infant milk according to claim 3 wherein the protease-digested milk material comprises one or more of raw cow's milk hydrolysate, hydrolyzed desalted whey powder, hydrolyzed concentrated whey protein powder.
5. The liquid infant milk of claim 4 wherein the raw milk hydrolysate has a polypeptide content of 3.0-9.0 mg/mL;
the polypeptide content of the hydrolyzed desalted whey powder is 12.0-36.0 mg/g;
the polypeptide content of the hydrolyzed concentrated whey protein powder is 80.0-392.0 mg/g.
6. Liquid infant milk according to any one of claims 1-5, wherein the raw material composition per 1000Kg of liquid infant milk comprises the following components: 128.5-320 Kg of raw milk hydrolysate, 20-90 Kg of hydrolyzed desalted whey powder, 0-18 Kg of hydrolyzed concentrated whey protein powder, 8.9-15.5 Kg of anhydrous cream, 10.0-15.4 Kg of vegetable oil, 0-25.4 Kg of lactose, 0.32-0.6 Kg of compound vitamin, 0.64-0.96 Kg of compound mineral, 0.075-0.115 Kg of choline, 0.065-0.095 Kg of taurine, 0.05-0.3 Kg of carrageenan, 0.05-0.25 Kg of citric acid and the balance of supplementing water to 1000Kg.
7. The liquid infant milk of claim 6 wherein the raw material composition of the liquid infant milk further adds nutrients that facilitate infant growth; the nutrient comprises DHA and lactoferrin;
the raw material composition of the liquid infant milk is also added with other food raw materials, emulsifying agents and/or stabilizing agents;
the other food raw materials comprise other protein raw materials and/or unsaturated fatty acid and phospholipid;
the emulsifier comprises glyceryl monostearate and/or citric acid fatty acid glyceride.
8. The method for preparing liquid infant milk according to any one of claims 1 to 7, comprising the steps of:
(1) Preparing or preparing materials for providing polypeptide and other raw materials, mixing, and supplementing total weight with water;
(2) Homogenizing the mixed material obtained in the step (1);
(3) Sterilizing the homogenized material in the step (2);
(4) And cooling the sterilized material to obtain the liquid infant milk.
9. The preparation method according to claim 8, wherein the step (2) is carried out under conditions of a primary homogenizing pressure of 15 to 20MPa and a secondary homogenizing pressure of 3 to 5 MPa.
10. The method according to claim 8, wherein the sterilization treatment is performed by UHT.
11. The preparation method of claim 10, wherein the preparation method is performed at 130-140 a o And C, treating for 2-5s to sterilize.
12. The preparation method according to any one of claims 8 to 11, wherein step (1) further comprises preparing the polypeptide-providing material, the preparation comprising the steps of:
(1.1) subjecting part or all of the milk material used to provide the protein in the liquid infant milk to a heat treatment to denature the protein therein, then cooling the solution to a temperature suitable for proteolytic hydrolysis, and adding water before or after cooling to form a system to be hydrolysed;
(1.2) adding protease into the system to be hydrolyzed obtained in the step (1.1) for hydrolysis, and thermally inactivating the protease after the hydrolysis to obtain the material for providing the polypeptide.
13. The method of manufacturing according to claim 12, wherein:
the protein concentration in the system to be hydrolyzed is 3.0% -20.0%;
the mass ratio of water in the system before adding protease is 80% -95%;
in the step (2), the protease is a compound protease which is formed by mixing one or more proteases in different proportions and meets the requirements of GB2760 on the proteases in food;
the added protease is hydrolyzed under the optimal temperature and pH condition of the protease; the hydrolysis time is that the content of polypeptide generated by hydrolysis reaches the content of polypeptide in the final product liquid infant milk.
14. The preparation method of claim 13, wherein the protease is a compound protease, and the compound protease comprises the following components in percentage by weight: validase FP Concentrate and Protease GBW of 1; the addition amount of the protease is 0.4% -0.9% of the mass of the protein substrate;
the hydrolysis time is 0.5-4.0 h.
15. The preparation method of claim 13, wherein the preparation method of the liquid infant milk comprises the steps of:
(1.1) mixing part or all of the milk material used to provide the protein in the liquid infant milk with water to prepare a solution, and performing a heat treatment to denature the protein therein, and then cooling the solution to a temperature suitable for proteolytic hydrolysis;
(1.2) adding protease into the system to be hydrolyzed obtained in the step (1.1) to hydrolyze at the optimal temperature and pH, and thermally inactivating the protease after hydrolysis to obtain a material for providing polypeptides;
(1.3) mixing the polypeptide-providing material obtained in step (1.2) with the remaining raw materials, and supplementing the total weight with water;
(2) Homogenizing the mixed material obtained in the step (1.3); homogenizing under the conditions that the primary homogenizing pressure is 15-20MPa and the secondary homogenizing pressure is 3-5 MPa;
(3) Sterilizing the homogenized material in the step (2) at 130-140 o Performing UHT sterilization under the condition of 2-5s of treatment under the condition of C;
(4) And cooling the sterilized material to obtain the liquid infant milk.
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| CN106605709A (en) * | 2015-10-22 | 2017-05-03 | 内蒙古伊利实业集团股份有限公司 | Liquid milk suitable for children at 3-6 years old and preparation method thereof |
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