CN110396475A - A kind of Penicillium notatum phosphorus decomposing agent and its preparation method and application - Google Patents
A kind of Penicillium notatum phosphorus decomposing agent and its preparation method and application Download PDFInfo
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- CN110396475A CN110396475A CN201910564182.8A CN201910564182A CN110396475A CN 110396475 A CN110396475 A CN 110396475A CN 201910564182 A CN201910564182 A CN 201910564182A CN 110396475 A CN110396475 A CN 110396475A
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- B—PERFORMING OPERATIONS; TRANSPORTING
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Abstract
The present invention provides a kind of Penicillium notatum phosphorus decomposing agent and its preparation method and application.The Penicillium notatum phosphorus decomposing agent lucky agriculture Penicillium notatum (Penicillium chinensis) TYPS-1 that deposit number is CGMCC No.17668 through the invention collects the mycelia concentrated product that sediment obtains after culture, fermentation, filtering, dissolving P capacity is strong, the indissoluble P elements around crop root can be decomposed, the phosphorus decomposing preparation as degradation soil indissoluble Phos.Wheat bran can be used, the agricultural wastes such as husk, which cultivate as raw material preparation culture medium, can obtain a large amount of thallus, so that production cost is reduced, and the technological process of production is simple, it is suitable for large-scale promotion.
Description
Technical field
The invention belongs to microbial manure technical field more particularly to a kind of Penicillium notatum phosphorus decomposing agent and preparation method thereof and answer
With.
Background technique
Phosphorus is one of mineral element necessary to plant growth, is plant nucleic acid in vivo and a variety of enzymes, coenzyme, Adenosine triphosphate
The important component of glycosides (ATP) etc., and the intracorporal photosynthesis of plant and physiological and biochemical procedure are participated in many ways, to rush
Growth and development and metabolism into plant play a significant role.Plant, which lacks phosphorus, will affect nucleic acid, protein synthesis, make cell shape
Be suppressed at proliferation, growth and development caused to be stagnated, root system development is bad, and plant is short and small, all kinds of crop tillers will reduce or
Delay, heading are postponed, are bloomed late, and mature slow, grain number per spike is reduced, and hollow kernels, corn ear is bald, and potato tubers becomes smaller
Deng.Therefore, phosphorus is very important for agricultural production.
The phosphorus library of nature is primarily present in soil, and circulation mainly carries out between soil, plant and microorganism.Soil
The height of supply source of the earth as plant phosphorus element, Phosphorus-supplying Level affects the growth and development of plant.Phosphorus in soil is containing organic
Phosphorus and two kinds of Phos, most of is insoluble phosphate (calcium phosphate, ferric phosphate, aluminate or phosphate etc.), these forms it is inorganic
Phosphide is difficult to be absorbed and used by plants, can only be accounted for by the available P that plant is directly absorbed and utilized in soil full phosphorus 2%~
3%.Most of phosphorus forms insoluble phosphorus in conjunction with Ca2+, Fe3+, Fe2+, Al3+ in soil due to the fixed function of soil
Hydrochlorate, therefore, most of phosphate fertilizer accumulate in the soil as invalid state (indissoluble state).Due to long-term application of P fertilizer, in fact greatly
Most potential phosphorus library contents of agricultural land soil are very big, and phosphate fertilizer excessive use will cause the increase of heavy metal content in soil, soil
Earth physicochemical property changes, and a large amount of applications of organophosphorus pesticide, causes environmental pollution, influences agricultural product quality, is good for the mankind
Health brings very big harm.
Phosphorus is the important limiting factor in many developing country's agricultural productions, and the utilization efficiency for improving phosphorus in soil will have
It is of great importance.The experimental results show that certain micro-organisms has the function of very strong phosphorus decomposing, by its secretion or absorb work
With that unavailable phosphorus in soil can be converted to available P, the material incentives plant growths such as auxin are also secreted.Phosphate solubilizing microorganism
Most direct effect is exactly slightly solubility or the insoluble phosphorus element dissolved in soil.Therefore, phosphate solubilizing microorganism is screened, production contains solution
The microbial organic fertilizer of phosphorus function is a good approach to plant phosphorus element supply problem is solved.
Phosphate solubilizing microorganism is divided into phosphate-solubilizing bacteria, phosphorus decomposing fungi and phosphorus decomposing actinomyces.Currently, majority is raw about phosphate-solubilizing bacteria
The research of object fertilizer, and it is relatively fewer about phosphorus decomposing fungal organism fertilizer；The shortcomings that phosphobacteria bio-fertilizer: (1) for cultivating phosphorus
Stringent sterile working must be carried out in bacteria culture media and incubation, be even so also easy by other fungal contaminations；(2)
Phosphobacteria easily it is affected by environment mutation and lose phosphorus decomposing effect；(3) C/N ratio must be suitable for during the cultivation process, otherwise shadow
Ring growth.(4) second level culture and third stage culture can only fluid nutrient medium culture, production technology is complicated, increases production cost.And
Phosphorus decomposing fungi knows that bacterium volume is smaller according to the characteristics of microorganism, easy mutation affected by environment, and fungi has
Genetic stability, and the feature that dissolving P capacity is strong.Currently, isolated phosphorus decomposing fungi mainly include mould, aspergillus, head mold,
Reaping hook is mould, small rhizoctonia etc., wherein reporting that more is aspergillus niger and mould.
Summary of the invention
In view of the above-mentioned state of the art, the present invention provides a kind of Penicillium notatum phosphorus decomposing agent and preparation method thereof and answer
With to solve drawbacks described above existing in the prior art.
The technical scheme of the invention to solve the technical problem is:
1. a kind of preparation method of Penicillium notatum phosphorus decomposing agent, comprising the following steps:
(1) slant strains: being made test tube slant with PDA solid medium, with oese from lucky agriculture Penicillium notatum
A small amount of mycelium is taken to cross on inclined-plane in (Penicillium chinensis) TYPS-1CGMCC No.17668 original seed, so
It cultivates at 28 DEG C afterwards, covers with inclined-plane；The PDA solid medium is made of following component based on mass fraction: Ma Ling
Potato 20g, glucose 2g, agar 1.5-2g, water 100mL, pH are natural；
(2) secondary liquid culture: being transferred to secondary liquid culture medium, in 28 DEG C, revolving speed 160rmin-1Under the conditions of shaking table
Culture；The secondary liquid culture medium is made of following component based on mass fraction: beancake powder 3%, sucrose 2%, peptone
0.2%, potassium dihydrogen phosphate 0.02%, magnesium sulfate 0.02%, water 100%, pH are natural；
(3) three-level liquid expands fermentation or three-level expands solid culture: being transferred to three-level fluid nutrient medium or three-level expands
Big solid medium, wherein when three-level Liquid Culture, in 28 DEG C, revolving speed 160rmin-1Under the conditions of shaking table culture；Three-level expands
When solid culture, cultivated at 28 DEG C；The three-level fluid nutrient medium is made of following component based on mass fraction: beancake powder
3%, sucrose 2%, peptone 0.2%, potassium dihydrogen phosphate 0.02%, magnesium sulfate 0.02%, water 100%, pH are natural；The three-level
Expand solid medium to be made of following component based on mass fraction: wheat bran 70%, rice husk 30%, amount of water are material weight
60%, pH is natural；
(4) it filters, taking precipitate；
(5) 40~45 DEG C of drying rapidly；
(6) finished product sterile packaged；Finished product water content is no more than 10%, 6~8 months effective storage periods.
2. a kind of Penicillium notatum phosphorus decomposing agent, the active constituent of the Penicillium notatum phosphorus decomposing agent is lucky agriculture Penicillium notatum (Penicillium
Chinensis) the mycelia of TYPS-1CGMCC No.17668.
3. a kind of Penicillium notatum phosphorus decomposing agent, the Penicillium notatum phosphorus decomposing agent passes through a kind of Penicillium notatum phosphorus decomposing described in technical solution 1
The preparation method of agent is prepared.
Application 4. Penicillium notatum phosphorus decomposing agent described in technical solution 2 or 3 is degraded in indissoluble Phos.
Compared with prior art, the invention has the following advantages:
The Penicillium notatum phosphorus decomposing agent of the present invention lucky agriculture Penicillium notatum that deposit number is CGMCC No.17668 through the invention
(Penicillium chinensis) TYPS-1 collects the mycelia concentration that sediment obtains after culture, fermentation, filtering and produces
Product can improve the utilization rate of the biological effectiveness of insoluble phosphorus and soil phophorus in soil, save fertilizer volume increase, improve soil environment, energy
Effectively facilitating plant includes absorption of the crops to soil P elements, decomposes the indissoluble P elements around crop root, Ke Yizuo
For the phosphorus decomposing preparation for soil indissoluble Phos of degrading.
In addition, needing to carry out culture medium in sterilizing and incubation before culture according to the Biological characteristics of microorganism
Sterile working is carried out, and the likelihood ratio bacterium that Penicillium notatum is polluted by other bacterium is low；Since Penicillium fungi is adaptable, poor
It can be mushroomed out in lean nutrition, so when three-level expands solid culture wheat bran can be used, the agricultural wastes such as husk are as raw material
Preparation culture medium, which cultivate, can obtain a large amount of thallus, to reduce production cost, and the technological process of production is simple, fit
Close a wide range of promote.
Detailed description of the invention
Fig. 1 is schematic diagram of the present invention.
Specific embodiment
, there are further understanding and understanding in the effect of to make to feature of the invention and being reached, preferably to implement
Example and attached drawing cooperation detailed description, each embodiment is conventional method without specified otherwise, is described as follows:
The Penicillium notatum of the Penicillium notatum phosphorus decomposing agent of the present invention of embodiment 1 describes:
Lucky agriculture Penicillium notatum (Penicillium chinensis) TYPS-1
Referred to as: CGMCC
Deposit number: 17668
Preservation date: on May 15th, 2019
The title of depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center
Preservation address: city, BeiJing, China, North Star West Road 1, Chaoyang District institute 3
Postcode: 100101
Ji agriculture Penicillium notatum (Penicillium chinensis) TYPS-1, which is used, contains indissoluble phosphorus culture medium, in pH9.0
Under the conditions of separate and obtain from the milpa soil of environment in Tongyu County of Jilin Province.
Morphological analysis: having conidial head, generates conidium, and conidiophore betides aerial hyphae, mitogenetic spore
Son stalk, metulae and conidial wall are significantly coarse, and most of broom shape branch is raw for two-wheel, have small part three verticillate and non-
Symmetrical, metulae is snugly into each other.Bacterium colony is cotton-shaped and velvet-like in PDA and CYA solid medium quality, and conidium structure is usual
Rareness, the white mycelium or pistac at edge, middle part are in celadon.
The preparation of the Penicillium notatum phosphorus decomposing agent phosphorus decomposing preparation of the present invention of embodiment 2
The preparation method of Penicillium notatum phosphorus decomposing agent of the present invention, comprising the following steps:
(1) slant strains: being made test tube slant with PDA solid medium, with oese from lucky agriculture Penicillium notatum
A small amount of mycelium is taken to cross on inclined-plane in (Penicillium chinensis) TYPS-1CGMCC No.17668 original seed, so
It cultivates at 28 DEG C afterwards, covers with inclined-plane；The PDA solid medium is made of following component based on mass fraction: Ma Ling
Potato 20g, glucose 2g, agar 1.5-2g, water 100mL, pH are natural；
(2) secondary liquid culture: being transferred to secondary liquid culture medium, in 28 DEG C, revolving speed 160rmin-1Under the conditions of shaking table
Culture；The secondary liquid culture medium is made of following component based on mass fraction: beancake powder 3%, sucrose 2%, peptone
0.2%, potassium dihydrogen phosphate 0.02%, magnesium sulfate 0.02%, water 100%, pH are natural；
(3) three-level liquid expands fermentation or three-level expands solid culture: being transferred to three-level fluid nutrient medium or three-level expands
Big solid medium, wherein when three-level Liquid Culture, in 28 DEG C, revolving speed 160rmin-1Under the conditions of shaking table culture；Three-level expands
When solid culture, cultivated at 28 DEG C；The three-level fluid nutrient medium is made of following component based on mass fraction: beancake powder
3%, sucrose 2%, peptone 0.2%, potassium dihydrogen phosphate 0.02%, magnesium sulfate 0.02%, water 100%, pH are natural；The three-level
Expand solid medium to be made of following component based on mass fraction: wheat bran 70%, rice husk 30%, amount of water are material weight
60%, pH is natural；
(4) it filters, taking precipitate；
(5) 40~45 DEG C of drying rapidly；
(6) finished product sterile packaged；Finished product water content is no more than 10%, 6~8 months effective storage periods.
The test of the Penicillium notatum dissolving P capacity of the present invention of embodiment 3
A small amount of strain is seeded in first after cultivating a certain concentration in 100mLPDA fluid nutrient medium, by 2% inoculum concentration
It is inoculated into containing indissoluble phosphorus Ca3(PO4)2Fluid nutrient medium culture 1d after, with molybdenum antimony resistance colorimetric method survey strain phosphorus decomposing amount；Knot
Fruit, compared with the control group for not connecing bacterium, when original fluid pH7.0, measuring titanium pigment content is 280.261 ± 1.11 μ g/mL；
As original fluid pH9.0, measuring titanium pigment content is 331.843 ± 3.41 μ g/mL.According to document announcement, under equal conditions,
Phosphorus decomposing amount of phosphobacteria 3-4d up to 600 μ g/mL or so, it is therefore seen that the dissolving P capacity of this Penicillium notatum is strong.
It should be noted that relational terms such as first and second and the like are used merely to an entity herein
Or operation is distinguished with another entity or operation, is existed without necessarily requiring or implying between these entities or operation
Any actual relationship or order.And the terms "include", "comprise" or its any other variant are intended to non-row
His property includes, so that the process, method, article or equipment for including a series of elements not only includes those elements, and
And further include other elements that are not explicitly listed, or further include for this process, method, article or equipment institute it is intrinsic
Element.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations；Although
Present invention has been described in detail with reference to the aforementioned embodiments, it will be understood by those of skill in the art that it still can be right
Technical solution documented by previous embodiment is modified, or is replaced on an equal basis to part of technical characteristic；And these
It modifies or replaces, the spirit and scope for technical solution of various embodiments of the present invention that it does not separate the essence of the corresponding technical solution.
Claims (2)
1. a kind of Penicillium notatum phosphorus decomposing agent, it is characterised in that: its active constituent is lucky agriculture Penicillium notatum (Penicillium
Chinensis) the mycelia of TYPS-1 CGMCC No.17668, the mycelia are prepared by following preparation method:
(1) slant strains: being made test tube slant with PDA solid medium, with oese from lucky agriculture Penicillium notatum (Penicillium
Chinensis it) takes a small amount of mycelium to cross on inclined-plane in TYPS-1 CGMCC No.17668 original seed, then cultivates at 28 DEG C,
Cover with inclined-plane；The PDA solid medium is made of following component based on mass fraction: potato 20g, glucose
2g, agar 1.5-2g, water 100mL, pH are natural；
(2) secondary liquid culture: being transferred to secondary liquid culture medium, in 28 DEG C, revolving speed 160rmin-1Under the conditions of shaking table culture；
The secondary liquid culture medium is made of following component based on mass fraction: beancake powder 3%, sucrose 2%, peptone 0.2%,
Potassium dihydrogen phosphate 0.02%, magnesium sulfate 0.02%, water 100%, pH are natural；
(3) three-level liquid expands fermentation or three-level expands solid culture: being transferred to three-level fluid nutrient medium or three-level expands admittedly
Body culture medium, wherein when three-level Liquid Culture, in 28 DEG C, revolving speed 160rmin-1Under the conditions of shaking table culture；Three-level expands solid
When culture, cultivated at 28 DEG C；The three-level fluid nutrient medium is made of following component based on mass fraction: beancake powder 3%,
Sucrose 2%, peptone 0.2%, potassium dihydrogen phosphate 0.02%, magnesium sulfate 0.02%, water 100%, pH are natural；The three-level expands
Solid medium is made of following component based on mass fraction: wheat bran 70%, rice husk 30%, amount of water be expect weight 60%,
PH is natural；
(4) it filters, taking precipitate；
(5) 40~45 DEG C of drying rapidly；
(6) finished product sterile packaged；Finished product water content is no more than 10%, 6~8 months effective storage periods.
2. a kind of application of the Penicillium notatum phosphorus decomposing agent according to claim 1 in degradation indissoluble Phos.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116536239A (en) * | 2023-04-17 | 2023-08-04 | 广州市林业和园林科学研究院 | Phosphate-dissolving microbial agent, and preparation and application thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116536239A (en) * | 2023-04-17 | 2023-08-04 | 广州市林业和园林科学研究院 | Phosphate-dissolving microbial agent, and preparation and application thereof |
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