CN110393822A - 胶原蛋白-羟基磷灰石复合小分子骨材料的制备方法 - Google Patents

胶原蛋白-羟基磷灰石复合小分子骨材料的制备方法 Download PDF

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CN110393822A
CN110393822A CN201810367110.XA CN201810367110A CN110393822A CN 110393822 A CN110393822 A CN 110393822A CN 201810367110 A CN201810367110 A CN 201810367110A CN 110393822 A CN110393822 A CN 110393822A
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sirna
collagen
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汤涛
李刚
邓艳
林思恩
宋艺
黄志超
万力
毕宏伟
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Hong Kong Saining Biotechnology Co ltd
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    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
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    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants

Abstract

本发明公开了胶原蛋白‑羟基磷灰石复合小分子骨材料的制备方法，包括如下步骤：(1)以磷酸水溶液为溶剂，配置I型胶原蛋白溶液；将Ca(OH)2在氮气保护下，溶于去离子水中，配置浓度为Ca2+悬浊液；将小干扰RNA溶于纯净水中，配置小干扰RNA水溶液；(2)将I型胶原蛋白溶液、Ca2+悬浊液和小干扰RNA水在搅拌下，同时滴加到容器中，有絮状沉淀产生，并缠绕在搅拌片上，滴加完毕继续搅拌，抽滤，用去离子水洗涤沉淀，将沉淀在模具中预成型，冷冻干燥，再等静压压制得到。本发明的方法可以将小干扰RNA包裹于胶原蛋白/羟基磷灰石复合中，并通过实验证明，小干扰RNA的释放能够发挥相应的作用。

Description

胶原蛋白-羟基磷灰石复合小分子骨材料的制备方法
技术领域
本发明涉及医用生物材料领域，具体地涉及一种胶原蛋白-羟基磷灰石复合小分子骨材料的制备方法。
背景技术
骨是一个动态和高度血管化的组织，在人的一生中持续重塑。骨的再生能力强，特别是在年轻人身上，意味着大多数骨折愈合良好，无需太多干预。尽管如此，在骨肿瘤切除和严重骨不连的骨折导致的大段骨缺损需要手术介入。目前治疗的金标准是“自体移植”，涉及从患者的非承重部位采集“供体”骨，并移植到缺陷部位。自体骨虽有良好的生物相容性和诱导性，但也会给患者带来巨大的痛苦，同时引起供骨区的炎症和并发症。异种骨及人工合成材料本身抗原性高、生物相容性差等，因此临床上对于人工合成的骨修复材料具有巨大的需求。人体自然骨中，无机成分主要是羟基磷灰石，占骨质量的65％左右；有机成分主要是胶原蛋白，占骨质量的34％。羟基磷灰石由于其良好的骨传导性和生物活性在骨替代物和填充物中应用广泛。而胶原蛋白具有多种生物功能基团，在组织支架中的应用中颇有潜力。
小干扰RNA(siRNA)是一类由20多个核苷酸组成的双链RNA分子，可以通过特异性降解靶基因的信使核糖核酸(messager RNA,mRNA)起到沉默基因表达的作用。这一过程被称为RNA干扰(RNA interference,RNAi)。与反义技术相比其抑制基因表达更有效且特异性强。该技术在抗肿瘤、抗病毒和基因功能研究的领域中都得到了广泛的应用。siRNA代表有希望的治疗技术，然而在向患者施用siRNA中还存在运输的障碍。需要一定的载体将siRNA运送到治疗部位。
发明内容
本发明的目的是克服现有技术的不足，提供一种胶原蛋白-羟基磷灰石复合小分子骨材料的制备方法。
本发明的技术方案概述如下：
胶原蛋白-羟基磷灰石复合小分子骨材料的制备方法，包括如下步骤：
(1)以0.05-2mol/L的磷酸水溶液为溶剂，配置浓度为5mg/ml的I型胶原蛋白溶液；将Ca(OH)2在氮气保护下，溶于去离子水中，配置浓度为0.05～2mol/L的Ca2+悬浊液；将小干扰RNA溶于纯净水中，配置浓度为50nmol/ml的小干扰RNA水溶液，所述小干扰RNA的长度为20到25个核苷酸；
(2)按体积比为1000：1500：1的比例，将I型胶原蛋白溶液、Ca2+悬浊液和小干扰RNA水在搅拌下，同时滴加到容器中，有絮状沉淀产生，并缠绕在搅拌片上，滴加完毕继续搅拌10-14小时，抽滤，用去离子水洗涤沉淀，将沉淀在模具中用2-8MPa压力下预成型，冷冻干燥，在100-300MPa下等静压压制得到致密坚硬的胶原蛋白/羟基磷灰石复合小分子骨材料。本发明的优点：
本发明的方法可以将小干扰RNA包裹于胶原蛋白/羟基磷灰石复合中，并通过实验证明，小干扰RNA的释放能够发挥相应的作用。
附图说明
图1荧光素酶和体内成像系统(IVIS)检测siRNA。
图2荧光显微镜下检测siRNA的释放。
具体实施方式
下面结合具体实施例，对本发明作进一步说明:
实施例1
胶原蛋白-羟基磷灰石复合小分子骨材料的制备方法，包括如下步骤：
(1)以1mol/L的磷酸水溶液为溶剂，配置浓度为5mg/ml的I型胶原蛋白溶液；将Ca(OH)2在氮气保护下，溶于去离子水中，配置浓度为1mol/L的Ca2+悬浊液；将小干扰RNA溶于纯净水中，配置浓度为50nmol/ml的小干扰RNA水溶液，所述小干扰RNA的长度为20个核苷酸；正义链CGCGCUGCUUCUACAGAAUG(SEQ ID NO.1)反义链CAUUCUGUAGAAGCAGCGCG(SEQ IDNO.2)
(2)按体积比为1000：1500：1的比例，将I型胶原蛋白溶液、Ca2+悬浊液和小干扰RNA水在搅拌下，同时滴加到容器中，有絮状沉淀产生，并缠绕在搅拌片上，滴加完毕继续搅拌12小时，抽滤，用去离子水洗涤沉淀，将沉淀在模具中用5MPa压力下预成型，冷冻干燥，在200MPa下等静压压制得到致密坚硬的胶原蛋白/羟基磷灰石复合小分子骨材料。
实施例2
胶原蛋白-羟基磷灰石复合小分子骨材料的制备方法，包括如下步骤：
(1)以0.05mol/L的磷酸水溶液为溶剂，配置浓度为5mg/ml的I型胶原蛋白溶液；将Ca(OH)2在氮气保护下，溶于去离子水中，配置浓度为0.05mol/L的Ca2+悬浊液；将小干扰RNA溶于纯净水中，配置浓度为50nmol/ml的小干扰RNA水溶液，所述小干扰RNA的长度为25个核苷酸；正义链GAGAGACUCAUGCAGGAGAAUGAUG(SEQ ID NO.3)反义链CUCUAUCAUUCUCCUGCAUGAGUCU(SEQ ID NO.4)
(2)按体积比为1000：1500：1的比例，将I型胶原蛋白溶液、Ca2+悬浊液和小干扰RNA水在搅拌下，同时滴加到容器中，有絮状沉淀产生，并缠绕在搅拌片上，滴加完毕继续搅拌10小时，抽滤，用去离子水洗涤沉淀，将沉淀在模具中用2MPa压力下预成型，冷冻干燥，在100MPa下等静压压制得到致密坚硬的胶原蛋白/羟基磷灰石复合小分子骨材料。
实施例3
胶原蛋白-羟基磷灰石复合小分子骨材料的制备方法，包括如下步骤：
(1)以2mol/L的磷酸水溶液为溶剂，配置浓度为5mg/ml的I型胶原蛋白溶液；将Ca(OH)2在氮气保护下，溶于去离子水中，配置浓度为2mol/L的Ca2+悬浊液；将小干扰RNA溶于纯净水中，配置浓度为50nmol/ml的小干扰RNA水溶液，所述小干扰RNA的长度为20个核苷酸；正义链CGCGCUGCUUCUACAGAAUG(SEQ ID NO.1)反义链CAUUCUGUAGAAGCAGCGCG(SEQ IDNO.2)
(2)按体积比为1000：1500：1的比例，将I型胶原蛋白溶液、Ca2+悬浊液和小干扰RNA水在搅拌下，同时滴加到容器中，有絮状沉淀产生，并缠绕在搅拌片上，滴加完毕继续搅拌14小时，抽滤，用去离子水洗涤沉淀，将沉淀在模具中用8MPa压力下预成型，冷冻干燥，在300MPa下等静压压制得到致密坚硬的胶原蛋白/羟基磷灰石复合小分子骨材料。
实施例4
将FAM荧光标记的小干扰RNA替换实施例1中的小干扰RNA，其它同实施例。胶原蛋白-羟基磷灰石复合小分子骨材料包裹有小干扰RNA，结果如图1所示。由于siRNA是被荧光标记的，如果含有siRNA，那么复合骨材料也一定能被荧光标记。因此，我们采用荧光素酶和体内成像系统(IVIS)检测复合骨材料所带的荧光状况。FAM是绿色荧光，由图1可见，材料中呈现大面积的绿色荧光区域，证明复合骨材料中包裹有siRNA。
将胶原蛋白-羟基磷灰石复合小分子骨材料切割成立方体状，每个单体重量为0.05克，将单体浸入到0.1mol/L的盐酸溶液中，在30min，2h，7h和24h的时间点吸取一滴溶液放置在荧光显微镜下观察，由图2可见，随着浸入时间的增加，绿色荧光的强度也增加，证明复合胶原蛋白-羟基磷灰石复合小分子骨材料降解伴随siRNA的释放。
序列表
<110> 香港賽寧生物工程技術有限公司
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Claims (1)

1.胶原蛋白-羟基磷灰石复合小分子骨材料的制备方法，其特征是包括如下步骤：
(1)以0.05-2mol/L的磷酸水溶液为溶剂，配置浓度为5mg/ml的I型胶原蛋白溶液；将Ca(OH)2在氮气保护下，溶于去离子水中，配置浓度为0.05～2mol/L的Ca2+悬浊液；将小干扰RNA溶于纯净水中，配置浓度为50nmol/ml的小干扰RNA水溶液，所述小干扰RNA的长度为20到25个核苷酸；
(2)按体积比为1000：1500：1的比例，将I型胶原蛋白溶液、Ca2+悬浊液和小干扰RNA水在搅拌下，同时滴加到容器中，有絮状沉淀产生，并缠绕在搅拌片上，滴加完毕继续搅拌10-14小时，抽滤，用去离子水洗涤沉淀，将沉淀在模具中用2-8MPa压力下预成型，冷冻干燥，在100-300MPa下等静压压制得到致密坚硬的胶原蛋白/羟基磷灰石复合小分子骨材料。
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