CN110387395A - The preparation method and selenium-rich solid beverage of mushroom with abundant selenium protein peptide powder - Google Patents
The preparation method and selenium-rich solid beverage of mushroom with abundant selenium protein peptide powder Download PDFInfo
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- CN110387395A CN110387395A CN201910787732.2A CN201910787732A CN110387395A CN 110387395 A CN110387395 A CN 110387395A CN 201910787732 A CN201910787732 A CN 201910787732A CN 110387395 A CN110387395 A CN 110387395A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C11/00—Milk substitutes, e.g. coffee whitener compositions
- A23C11/02—Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins
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- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
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- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
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- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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Abstract
The present invention discloses the preparation method and selenium-rich solid beverage of a kind of mushroom with abundant selenium protein peptide powder, is related to food processing technology field.After the preparation method of the mushroom with abundant selenium protein peptide powder into mushroom with abundant selenium powder the following steps are included: add water and stir, cellulase is added and is digested, collects enzymatic hydrolysis supernatant；Alcohol precipitation after enzymatic hydrolysis supernatant concentration is handled, precipitating drying is taken, obtains mushroom protein；The mushroom protein is dissolved in water, compound protease is added, is filtered after enzymatic hydrolysis and collects filtrate；Into filter residue plus water extracts, and obtains extracting solution after filtering；After the filtrate is mixed with the extracting solution, membrane separation is carried out, obtains refined solution；The refined solution is sterilized, is dried, mushroom with abundant selenium protein peptide powder is obtained；Wherein, the compound protease is made of alkali protease, neutral proteinase and papain.Present invention seek to address that the problem that product bioavilability made from traditional protein peptide preparation method is low.
Description
Technical field
The present invention relates to food processing technology field, in particular to a kind of the preparation method and richness of mushroom with abundant selenium protein peptide powder
Selenium solid beverage.
Background technique
Protein is the important component of group adult body all cells, tissue, and all important component parts of body require
There is the participation of protein.Recent scientific research discovery, absorption of human body protein are mainly absorbed in the form of small peptide, and small peptide is by people
Body can be utilized directly by human body cell, blood and tissue after absorbing, and compared amino acid and be easier to quickly to be absorbed by the body and benefit
With.
Traditional protein peptides preparation method has DNA recombination method and enzyme process peptide, the former be of limited application and cost compared with
Height, although cost is relatively low by the latter, the peptide chain of preparation is longer, and small-molecular peptides yield is lower, and bioavilability is lower.
Summary of the invention
The main object of the present invention is to propose the preparation method and selenium-rich solid beverage of a kind of mushroom with abundant selenium protein peptide powder, purport
Solving the problems, such as that product bioavilability made from traditional protein peptide preparation method is low.
To achieve the above object, the present invention proposes a kind of preparation method of mushroom with abundant selenium protein peptide powder, the mushroom with abundant selenium
The preparation method of protein peptide powder the following steps are included:
After being added water and stirred into mushroom with abundant selenium powder, cellulase is added and is digested, collects enzymatic hydrolysis supernatant；
Alcohol precipitation after enzymatic hydrolysis supernatant concentration is handled, precipitating drying is taken, obtains mushroom protein；
The mushroom protein is dissolved in water, compound protease is added, is filtered after enzymatic hydrolysis and collects filtrate；
Into filter residue plus water extracts, and obtains extracting solution after filtering；
After the filtrate is mixed with the extracting solution, membrane separation is carried out, obtains refined solution；
The refined solution is sterilized, is dried, mushroom with abundant selenium protein peptide powder is obtained；
Wherein, the compound protease is made of alkali protease, neutral proteinase and papain.
Optionally, in the compound protease each component weight percent are as follows: it is alkali protease 10%~60%, neutral
Protease 10%~75% and papain 15%~80%.
Optionally, it is described added water and stirred into mushroom with abundant selenium powder after, be added cellulase digested, collect enzymatic hydrolysis supernatant
In the step of liquid, the partial size of the mushroom with abundant selenium powder is 10~40 μm；And/or
The additional amount of the cellulase is 200U/g~2000U/g.
Optionally, the mushroom protein is dissolved in water, compound protease is added, is filtered after enzymatic hydrolysis and collects filtrate
The step of in, the additional amount of the compound protease is 1000U/g~100000U/g.
Optionally, the mushroom protein is dissolved in water, compound protease is added, is filtered after enzymatic hydrolysis and collects filtrate
The step of in, the condition of the enzymatic hydrolysis are as follows: temperature 45 C~55 DEG C, pH7~8；And/or
The time of the enzymatic hydrolysis is 2~6h.
Optionally, after the filtrate being mixed with the extracting solution, carry out membrane separation, obtain refined solution the step of wrap
It includes:
The filtrate is mixed with the extracting solution, obtains mixed liquor；
Constant volume ultrafiltration is carried out to the mixed liquor using the ultrafiltration membrane that molecular cut off is 5kDa~10kDa, obtains ultrafiltration
Liquid；
The nanofiltration membrane constant volume nanofiltration for being 1kDa~3kDa by molecular cut off by the ultrafiltrate, obtains refined solution.
Optionally, described that constant volume is carried out to the mixed liquor using the ultrafiltration membrane that molecular cut off is 5kDa~10kDa
Ultrafiltration, in the step of obtaining ultrafiltrate, the condition of the constant volume ultrafiltration are as follows: ultrafiltration pressure is 0.16MPa~0.19MPa, temperature
20~25 DEG C.
Optionally, the nanofiltration membrane constant volume nanofiltration for being 1kDa~3kDa by molecular cut off by the ultrafiltrate, obtains pure
In the step of changing liquid, the condition of the constant volume nanofiltration are as follows: pressure is 1.9MPa~2.1MPa, 20~25 DEG C of temperature.
In addition, the present invention also proposes that a kind of selenium-rich solid beverage, the selenium-rich solid beverage include following parts by weight
Component: 600~800 parts of selenium-enriched protein peptide, soybean milk powder 60 made from the preparation method of mushroom with abundant selenium protein peptide powder as described above
~120 parts, 5~20 parts of Chinese yam polysaccharide, 20~30 parts of red date powder, 15~30 parts of Cordyceps militaris powder, 90~180 parts of white granulated sugar, salt 9
~18 parts, 1~2 part of soybean lecithin and 0.04~0.05 part of vitamin E.
Optionally, the selenium-rich solid beverage is tablet or pulvis.
It is compound using being made of alkali protease, neutral proteinase and papain in technical solution of the present invention
Protease is digested, and since these three protease have different action sites, three's combination can be made through cellulose
The protein that enzyme digests is fully hydrolyzed into small-molecular peptides, and it is more preferable to compare existing protease hydrolyzed effect；Meanwhile by multienzyme
Compound, stepwise discretization and membrane separation technique combination, also further improve the content of small-molecular peptides, are conducive to body and absorb, from
And the active constituent content of product is improved, improve the bioavilability of mushroom with abundant selenium protein peptide powder.In addition, the present invention uses
The mushroom with abundant selenium that selenium rich ability is preferable and nutritive value is high makes similarly to contain organic selenium in product as raw material, improves production
The nutritive value of product.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with
Other relevant attached drawings are obtained according to these attached drawings.
Fig. 1 is the flow diagram of an embodiment of mushroom with abundant selenium method for preparing protein peptide powder proposed by the present invention.
The embodiments will be further described with reference to the accompanying drawings for the realization, the function and the advantages of the object of the present invention.
Specific embodiment
It in order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below will be in the embodiment of the present invention
Technical solution be clearly and completely described.The person that is not specified actual conditions in embodiment, according to normal conditions or manufacturer builds
The condition of view carries out.Reagents or instruments used without specified manufacturer is the conventional production that can be obtained by commercially available purchase
Product.
In addition, the meaning of the "and/or" occurred in full text, including three schemes arranged side by side, by taking " A and/or B " as an example, packet
The scheme for including A scheme or B scheme or A and B while meeting.
Traditional protein peptides preparation method has DNA recombination method and enzyme process peptide, the former be of limited application and cost compared with
Height, although cost is relatively low by the latter, the peptide chain of preparation is longer, and small-molecular peptides yield is lower, is unfavorable for body absorption.
In consideration of it, the present invention proposes a kind of preparation method of mushroom with abundant selenium protein peptide powder, traditional protein peptide system can solve
The problem of Preparation Method small molecule low output.Fig. 1 is an embodiment of mushroom with abundant selenium method for preparing protein peptide powder proposed by the present invention.
In conjunction with Fig. 1 it is found that the preparation method of the mushroom with abundant selenium protein peptide powder the following steps are included:
Step S10, after being added water and stirred into mushroom with abundant selenium powder, cellulase is added and is digested, collect enzymatic hydrolysis supernatant.
Selenium is trace mineral element needed by human, is the main active of glutathione peroxidase, and human body lacks
Selenium can suffer from Keshan disease, Kaschin-Beck disease and gastric cancer etc..The existence form of selenium has two class of inorganic selenium and organic selenium.Compared with inorganic selenium,
Organic selenium is present in selenium-rich animals and plants, has the characteristics that absorptivity is high, bioactivity is strong, toxicity is low, environmental pollution is small.
The selenium rich ability of edible mushroom is generally better than general plant, and selenium rich ability not only has with the Se content in growth substrate
It closes, and has very big difference because of strain properties.Meanwhile after edible mushroom passes through selenium-enriching cultivation, its own active medicinal matter water
It is flat to improve.Using sodium selenite culture oyster mushroom, in general, the mycelial selenium of mushroom with abundant selenium contains under optimal conditions of fermentation
Amount can achieve 331.49~371.1mg/kg.In addition, one of the common edible mushroom that oyster mushroom sheet is high as full of nutrition, yield,
Nutritional characteristic with high protein and low fat, and can produce a variety of biologically active secondary metabolites, it can be to body
Generate beneficial effect.Moreover, the organic selenium contained in mushroom with abundant selenium mainly exists in the form of selenoprotein, by preparation side of the invention
Selenium element can be smoothly introduced into product by the processing of method.
In the present embodiment, the organic selenium that nutritive value is high, selenium rich ability is preferable and contains is used to exist in the form of selenoprotein
Mushroom with abundant selenium as raw material, make similarly to contain selenium in product, improve the nutritive value of product, so that product is in addition to being used as
Outside protein supplement, Selenium supplement agent is also acted as.
Sufficiently to be digested convenient for mushroom with abundant selenium powder by cellulase, in the present embodiment, first mushroom with abundant selenium is dried, powder
Broken processing, to obtain the mushroom with abundant selenium powder that partial size is 10~40 μm.At the same time it can also control the additional amount of cellulase, work as fibre
When the additional amount for tieing up plain enzyme is 200U/g~2000U/g, not only mushroom with abundant selenium powder can be made sufficiently to be digested, but also be avoided that cellulose
The waste of enzyme.It is to be appreciated that above-mentioned 200U/g~2000U/g refers to addition 200U~2000U fiber in every gram of mushroom with abundant selenium powder
Plain enzyme.
In addition, in the present embodiment, cellulase degradation condition is set for the effect of raising enzymatic hydrolysis are as follows: temperature 50 C~
55 DEG C, pH4~6,2~4h of enzymolysis time.
Step S20, alcohol precipitation after enzymatic hydrolysis supernatant concentration is handled, takes precipitating drying, obtains mushroom protein.
Specifically, after enzymatic hydrolysis supernatant being concentrated under reduced pressure, dehydrated alcohol ethyl alcohol into concentrate is added into concentrate
After volumetric concentration reaches 70%, centrifugation discards supernatant liquid, collects and precipitates and dry to get mushroom protein.
Step S30, the mushroom protein is dissolved in water, compound protease is added, is filtered after enzymatic hydrolysis and collects filter
Liquid.
Wherein, the compound protease is made of alkali protease, neutral proteinase and papain.
In the present embodiment, alkali protease, neutral proteinase and papain are mixed and made into compound protease, and
It is used for the compound protease to digest mushroom protein, since alkali protease, neutral proteinase and papain all have
Different action sites can act on the different peptide bonds of mushroom protein, and three's combination can sufficiently be divided mushroom protein
Solution compares existing protease at the lesser small-molecular peptides of molecular weight, and not only accounting is high for the small-molecular peptides digested, but also molecule
Measure it is small, have better hydrolysis result.Moreover, because the molecular weight of small-molecular peptides is smaller, also more conducively body absorbs.Specific
When implementation, first alkali protease, neutral proteinase and papain can be mixed compound before step S20
It is spare after protease.
Further, to cook up the lesser small-molecular peptides product of molecular weight, the present embodiment is to each group in compound protease
The weight percent divided limits, and specifically, the weight percent of each component is respectively as follows: alkali protease in compound protease
10%~60%, neutral proteinase 10%~75% and papain 15%~80%.That is, in compound protease
The percentage composition of alkali protease can be 10%, 12%, 15%, 20%, 30%, 40%, 50%, 60% etc.；Compound protein
In enzyme the percentage composition of neutral proteinase can for 10%, 15%, 20%, 30%, 35%, 40%, 50%, 60%, 70%,
75% etc.；In compound protease the percentage composition of papain can for 15%, 20%, 30%, 40%, 50%, 60%,
70%, 80% etc.；And the sum of weight percent of alkali protease, neutral proteinase and papain is 100%.
To digest mushroom protein sufficiently, in the present embodiment, the additional amount of compound protease is 1000U/g~100000U/
G, that is, 1000U~100000U compound protease is added in every gram of mushroom protein.
In addition, in the present embodiment, the enzymatic hydrolysis condition of compound protease is set are as follows: temperature 45 for the effect of raising enzymatic hydrolysis
DEG C~55 DEG C, pH7~8, enzymolysis time be 2~6h.
Step S40, into filter residue plus water extracts, and obtains extracting solution after filtering.
Due to equally in the present embodiment, further being extracted to filter residue containing some protein peptides to avoid wasting in filter residue,
To obtain extracting solution, to further improve the yield of protein peptides.
Step S50, after mixing the filtrate with the extracting solution, membrane separation is carried out, obtains refined solution.
In the present embodiment, purifies and separates are carried out using membrane separation technique, plasma selenium and protein peptides will not be destroyed, to have
Effect improves the content of protein peptides and selenium in refined solution.
When it is implemented, step S50 may comprise steps of:
Step S51, the filtrate is mixed with the extracting solution, obtains mixed liquor.
Step S52, constant volume is carried out to the mixed liquor using the ultrafiltration membrane that molecular cut off is 5kDa~10kDa to surpass
Filter, obtains ultrafiltrate.
It wherein, can be by the condition setting of constant volume ultrafiltration when carrying out constant volume ultrafiltration are as follows: ultrafiltration pressure is
20~25 DEG C of 0.16MPa~0.19MPa, temperature.
Step S53, the nanofiltration membrane constant volume nanofiltration for being 1kDa~3kDa by molecular cut off by the ultrafiltrate, obtains pure
Change liquid.
Wherein, can be by the condition setting of constant volume nanofiltration when carrying out constant volume nanofiltration are as follows: pressure be 1.9MPa~
20~25 DEG C of 2.1MPa, temperature.
Step S60, the refined solution sterilized, dried, obtain mushroom with abundant selenium protein peptide powder.
Wherein, the mode of drying process can be natural drying, freeze-drying or vacuum drying etc., in the present embodiment,
By the way of vacuum freeze drying, specifically, the condition of vacuum freeze drying are as follows: vacuum degree 15Pa~20Pa, -75 DEG C of temperature
~-80 DEG C, drying time for 24 hours~30h.
In addition, being based on above-mentioned preparation method embodiment, the present invention also proposes a kind of selenium-rich solid beverage, the selenium-rich solid
Beverage includes the component of following parts by weight: mushroom with abundant selenium made from the preparation method of mushroom with abundant selenium protein peptide powder as described above
600~800 parts of protein peptide powder, 60~120 parts of soybean milk powder, 5~20 parts of Chinese yam polysaccharide, 20~30 parts of red date powder, Cordyceps militaris powder 15
~30 parts, 90~180 parts of white granulated sugar, 9~18 parts of salt, 1~2 part of soybean lecithin and 0.04~0.05 part of vitamin E.
Wherein, vitamin E and organic selenium have synergistic effect, and the two use simultaneously can be improved anti-oxidation efficacy, prevent
Free radical is formed, and has protective action to hepatopathy；Chinese yam polysaccharide extracts from Chinese yam stem tuber, can voluntarily prepare, and can also buy
Arrive, Chinese yam polysaccharide has effects that enhance immune, antitumor, anti-aging and oxidation resistant, can be nourishing liver and kidney, and to immunity with
Chemical damage has significant protective effect；In addition, jujube is rich in oleanolic acid, ursolic acid and betulic acid, wherein neat
Pier tartaric acid and ursolic acid have significant liver protecting effect, and red date powder is to be milled to be made by dry jujube.
In addition, it should be noted that, above-mentioned soybean milk powder can be the soybean milk powder of various beans, such as soya bean soybean milk powder, green
Beans soybean milk powder, red bean soybean milk powder etc., in the present embodiment, preferably mouthfeel is strong, compatibility is good and yield is big soya bean soybean milk powder.
It is more that vitamin E, Chinese yam are added in the present embodiment, in mushroom with abundant selenium protein peptide powder made from Xiang Shangshu preparation method
The components such as sugar, red date powder, are made solid beverage, the solid beverage be rich in be easy to absorb small molecular protein peptide, selenium element and its
His a variety of effective nutritional ingredients can supplement protein and selenium element, but also bioavilability with higher not only for human body,
And also there is oxidation resistant effect, free radical, the resistance strengthened liver plasma membrane, enhance liver, the damage for reducing liver can be removed,
Promote health, is a kind of to have effects that nourishing and protecting liver, food full of nutrition.
Further, the way of realization of above-mentioned selenium-rich solid beverage can there are many, such as bulk, granule, pulvis or
The solid beverage of the solubility such as person's tablet.In the present embodiment, selenium-rich solid beverage can be tablet or pulvis, wherein tablet
It is small in size, easy to package and have the longer shelf-life；Pulvis then has the advantages that preparation process is simple, dissolubility is good.
In addition, for above-mentioned selenium-rich solid beverage, it, can be using being made following preparation method, specifically by taking pulvis as an example
The following steps are included:
Step 1: by mushroom with abundant selenium protein peptide powder, Chinese yam polysaccharide, red date powder, Cordyceps militaris powder, white granulated sugar, salt, soybean phosphorus
After rouge and vitamin E add water to mix, homogeneous is carried out, must be homogenized；
Step 2: homogenate is spray-dried, it is so cooling that just to match powder；
Step 3: will just be uniformly mixed, encapsulate up to selenium-rich solid beverage powder with soybean milk powder with powder under oxygen-free environment.
It is understood that tablet is made if necessary, then obtained selenium-rich solid need to only be drunk when carrying out step 3
The further compressing tablet process of feed powder is to get selenium-rich solid beverage tablet.
Technical solution of the present invention is described in further detail below in conjunction with specific embodiment, it should be understood that following real
It applies example to be only used to explain the present invention, be not intended to limit the present invention.
Embodiment 1
By mushroom with abundant selenium after dry, pulverization process, the mushroom with abundant selenium powder that partial size is 10 μm is made.Take 100g mushroom with abundant selenium powder
It is added in 1000g water, adds 20000U cellulase, digest 3h under the conditions of 55 DEG C, pH5, obtain enzymatic hydrolysis supernatant.By enzyme
It solves after supernatant is concentrated under reduced pressure, dehydrated alcohol is added into concentrate after the volumetric concentration of ethyl alcohol reaches 70% into concentrate,
Centrifugation discards supernatant liquid, collects and precipitates and dry to get mushroom protein.
Mushroom protein is dissolved in water, and by 1000U/g mushroom protein additive amount be added compound protease, in 55 DEG C,
2h is digested under the conditions of pH8, and filtrate, and the extracting in water again into the filter residue being collected into then is collected by filtration, obtains after filtering
Extracting solution.Wherein, in compound protease, alkali protease weight percentage is 10%；Neutral proteinase weight percentage
It is 10%；Papain weight percentage is 80%.
After filtrate is mixed with extracting solution, the ultrafiltration membrane for the use of molecular cut off being 10kDa, in 0.16MPa, 25 DEG C of item
Constant volume ultrafiltration is carried out to mixed liquor under part, obtains ultrafiltrate；Then the nanofiltration membrane for the use of molecular cut off being 1kDa, In
1.9MPa, constant volume nanofiltration is carried out to ultrafiltrate under conditions of 21 DEG C, obtains refined solution.
After sterilizing to refined solution, vacuum freeze drying is carried out under the conditions of 15Pa, -80 DEG C, obtains mushroom with abundant selenium albumen
Gly-His-Lys.
Embodiment 2
By mushroom with abundant selenium after dry, pulverization process, the mushroom with abundant selenium powder that partial size is 20 μm is made.Take 100g mushroom with abundant selenium powder
It is added in 1000g water, adds 200000U cellulase, digest 4h under the conditions of 50 DEG C, pH4, obtain enzymatic hydrolysis supernatant.It will
After digesting supernatant reduced pressure, dehydrated alcohol volumetric concentration of ethyl alcohol into concentrate is added into concentrate and reaches 70%
Afterwards, centrifugation discards supernatant liquid, collects and precipitates and dry to get mushroom protein.
Mushroom protein is dissolved in water, and by 5000U/g mushroom protein additive amount be added compound protease, in 45 DEG C,
4h is digested under the conditions of pH7, and filtrate, and the extracting in water again into the filter residue being collected into then is collected by filtration, obtains after filtering
Extracting solution.Wherein, in compound protease, alkali protease weight percentage is 60%；Neutral proteinase weight percentage
It is 20%；Papain weight percentage is 20%.
After filtrate is mixed with extracting solution, the ultrafiltration membrane for the use of molecular cut off being 5kDa, in 0.17MPa, 21 DEG C of item
Constant volume ultrafiltration is carried out to mixed liquor under part, obtains ultrafiltrate；Then the nanofiltration membrane for the use of molecular cut off being 2kDa, In
2.0MPa, constant volume nanofiltration is carried out to ultrafiltrate under conditions of 22 DEG C, obtains refined solution.
After sterilizing to refined solution, vacuum freeze drying is carried out under the conditions of 16Pa, -75 DEG C, obtains mushroom with abundant selenium albumen
Gly-His-Lys.
Embodiment 3
By mushroom with abundant selenium after dry, pulverization process, the mushroom with abundant selenium powder that partial size is 40 μm is made.Take 100g mushroom with abundant selenium powder
It is added in 1000g water, adds 50000U cellulase, digest 2h under the conditions of 52 DEG C, pH6, obtain enzymatic hydrolysis supernatant.By enzyme
It solves after supernatant is concentrated under reduced pressure, dehydrated alcohol is added into concentrate after the volumetric concentration of ethyl alcohol reaches 70% into concentrate,
Centrifugation discards supernatant liquid, collects and precipitates and dry to get mushroom protein.
Mushroom protein is dissolved in water, and by 10000U/g mushroom protein additive amount be added compound protease, in 50 DEG C,
6h is digested under the conditions of pH7.3, and filtrate, and the extracting in water again into the filter residue being collected into then is collected by filtration, obtains after filtering
Obtain extracting solution.Wherein, in compound protease, alkali protease weight percentage is 50%；Neutral proteinase weight percent contains
Amount is 30%；Papain weight percentage is 20%.
After filtrate is mixed with extracting solution, the ultrafiltration membrane for the use of molecular cut off being 8kDa, in 0.18MPa, 22 DEG C of item
Constant volume ultrafiltration is carried out to mixed liquor under part, obtains ultrafiltrate；Then the nanofiltration membrane for the use of molecular cut off being 1kDa, In
2.1MPa, constant volume nanofiltration is carried out to ultrafiltrate under conditions of 23 DEG C, obtains refined solution.
After sterilizing to refined solution, vacuum freeze drying is carried out under the conditions of 17Pa, -78 DEG C, obtains mushroom with abundant selenium albumen
Gly-His-Lys.
Embodiment 4
By mushroom with abundant selenium after dry, pulverization process, the mushroom with abundant selenium powder that partial size is 30 μm is made.Take 100g mushroom with abundant selenium powder
It is added in 1000g water, adds 100000U cellulase, digest 2.5h under the conditions of 53 DEG C, pH4.2, obtain enzymatic hydrolysis supernatant
Liquid.After supernatant reduced pressure will be digested, dehydrated alcohol volumetric concentration of ethyl alcohol into concentrate is added into concentrate and reaches
After 70%, centrifugation discards supernatant liquid, collects and precipitates and dry to get mushroom protein.
Mushroom protein is dissolved in water, and by 50000U/g mushroom protein additive amount be added compound protease, in 52 DEG C,
5h is digested under the conditions of pH7.5, and filtrate, and the extracting in water again into the filter residue being collected into then is collected by filtration, obtains after filtering
Obtain extracting solution.Wherein, in compound protease, alkali protease weight percentage is 36%；Neutral proteinase weight percent contains
Amount is 14%；Papain weight percentage is 50%.
After filtrate is mixed with extracting solution, the ultrafiltration membrane for the use of molecular cut off being 7kDa, in 0.19MPa, 23 DEG C of item
Constant volume ultrafiltration is carried out to mixed liquor under part, obtains ultrafiltrate；Then using molecular cut off be 1kDa nanofiltration membrane, 2MPa,
Constant volume nanofiltration is carried out to ultrafiltrate under conditions of 24 DEG C, obtains refined solution.
After sterilizing to refined solution, vacuum freeze drying is carried out under the conditions of 18Pa, -77 DEG C, obtains mushroom with abundant selenium albumen
Gly-His-Lys.
Embodiment 5
By mushroom with abundant selenium after dry, pulverization process, the mushroom with abundant selenium powder that partial size is 25 μm is made.Take 100g mushroom with abundant selenium powder
It is added in 1000g water, adds 80000U cellulase, digest 3.5h under the conditions of 54 DEG C, pH5.5, obtain enzymatic hydrolysis supernatant.
After supernatant reduced pressure will be digested, dehydrated alcohol volumetric concentration of ethyl alcohol into concentrate is added into concentrate and reaches 70%
Afterwards, centrifugation discards supernatant liquid, collects and precipitates and dry to get mushroom protein.
Mushroom protein is dissolved in water, and compound protease is added by the additive amount of 100000U/g mushroom protein, in 48
DEG C, digest 3h under the conditions of pH7.8, filtrate, and the extracting in water again into the filter residue being collected into then is collected by filtration, after filtering
Obtain extracting solution.Wherein, in compound protease, alkali protease weight percentage is 10%；Neutral proteinase weight percent
Content is 75%；Papain weight percentage is 15%.
After filtrate is mixed with extracting solution, the ultrafiltration membrane for the use of molecular cut off being 6kDa, in 0.16MPa, 24 DEG C of item
Constant volume ultrafiltration is carried out to mixed liquor under part, obtains ultrafiltrate；Then using molecular cut off be 3kDa nanofiltration membrane, 2MPa,
Constant volume nanofiltration is carried out to ultrafiltrate under conditions of 25 DEG C, obtains refined solution.
After sterilizing to refined solution, vacuum freeze drying is carried out under the conditions of 20Pa, -79 DEG C, obtains mushroom with abundant selenium albumen
Gly-His-Lys.
(1) Determination of Selenium
The mushroom with abundant selenium protein peptide powder for taking each embodiment preparation, according to GB 5009.93-2017 national food safety standard
Measurement the first method Hydride atomic fluorescent spectrum method for detecting Se content of selenium in food simultaneously records as shown in table 1.
1 Se content of table
From upper table 1 it is found that the mushroom with abundant selenium protein peptide powder of each embodiment preparation all has higher Se content, illustrate this hair
The mushroom with abundant selenium protein peptide powder of bright preparation method preparation is rich in selenium.
(2) small-molecular peptides assay
Comparative example 1: in addition to changing compound protease into papain, other steps are same as Example 1.
Comparative example 2: except compound protease is changed into be made of 65% alkali protease and 35% papain it is compound
Outside protease, other steps are same as Example 1.
Due to gained mushroom with abundant selenium protein peptide powder, that is, target small-molecular peptides after 1kDa nanofiltration membrane nanofiltration, comparative example is taken
The mushroom with abundant selenium protein peptide powder prepared with embodiment 1 measures mushroom with abundant selenium under each preparation method using Micro-kjoldahl method
The yield of protein peptide powder (small-molecular peptides):
2 small-molecular peptides yield of table
| Embodiment 1 | Comparative example 1 | Comparative example 2 | |
| Small-molecular peptides yield (%) | 100 | 50 | 75 |
Note: being in terms of 100% by 1 gained small-molecular peptides yield of embodiment.
From upper table 2 it is found that comparing comparative example 1 and comparative example 2, the small molecule mushroom with abundant selenium protein peptide powder of the preparation of embodiment 1
Yield is considerably higher, that is to say, that mushroom with abundant selenium protein peptide powder prepared by preparation method of the present invention has high bioavilability.
Mushroom with abundant selenium protein peptide powder made from 1 based on the above embodiment, embodiment 6 to embodiment 10 have shown in the following table 3
Formula, mushroom with abundant selenium protein peptide powder shown in table 3 is made by 1 preparation method of above-described embodiment.
Each embodiment selenium-rich solid beverage formula table (g) of table 3
The above is only a preferred embodiment of the present invention, is not intended to limit the scope of the invention, for this field
For technical staff, the invention may be variously modified and varied.All within the spirits and principles of the present invention, made any
Modification, equivalent replacement, improvement etc. should all be included within the scope of the present invention.
Claims (10)
1. a kind of preparation method of mushroom with abundant selenium protein peptide powder, which comprises the following steps:
After being added water and stirred into mushroom with abundant selenium powder, cellulase is added and is digested, collects enzymatic hydrolysis supernatant；
Alcohol precipitation after enzymatic hydrolysis supernatant concentration is handled, precipitating drying is taken, obtains mushroom protein；
The mushroom protein is dissolved in water, compound protease is added, is filtered after enzymatic hydrolysis and collects filtrate；
Into filter residue plus water extracts, and obtains extracting solution after filtering；
After the filtrate is mixed with the extracting solution, membrane separation is carried out, obtains refined solution；
The refined solution is sterilized, is dried, mushroom with abundant selenium protein peptide powder is obtained；
Wherein, the compound protease is made of alkali protease, neutral proteinase and papain.
2. the preparation method of mushroom with abundant selenium protein peptide powder as described in claim 1, which is characterized in that in the compound protease
The weight percent of each component are as follows: alkali protease 10%~60%, neutral proteinase 10%~75% and papain
15%~80%.
3. the preparation method of mushroom with abundant selenium protein peptide powder as described in claim 1, which is characterized in that described to mushroom with abundant selenium powder
In add water and stir after, be added cellulase digested, collect enzymatic hydrolysis supernatant the step of in, the partial size of the mushroom with abundant selenium powder
It is 10~40 μm；And/or
The additional amount of the cellulase is 200U/g~2000U/g.
4. the preparation method of mushroom with abundant selenium protein peptide powder as described in claim 1, which is characterized in that add the mushroom protein
In the step of water dissolution is added compound protease, is filtered after enzymatic hydrolysis and collects filtrate, the additional amount of the compound protease
For 1000U/g~100000U/g.
5. the preparation method of mushroom with abundant selenium protein peptide powder as described in claim 1, which is characterized in that add the mushroom protein
In the step of water dissolution is added compound protease, is filtered after enzymatic hydrolysis and collects filtrate, the condition of the enzymatic hydrolysis are as follows: temperature
45 DEG C~55 DEG C, pH7~8；And/or
The time of the enzymatic hydrolysis is 2~6h.
6. the preparation method of mushroom with abundant selenium protein peptide powder as described in claim 1, which is characterized in that by the filtrate with it is described
Extracting solution mixing after, carry out membrane separation, obtain refined solution the step of include:
The filtrate is mixed with the extracting solution, obtains mixed liquor；
Constant volume ultrafiltration is carried out to the mixed liquor using the ultrafiltration membrane that molecular cut off is 5kDa~10kDa, obtains ultrafiltrate；
The nanofiltration membrane constant volume nanofiltration for being 1kDa~3kDa by molecular cut off by the ultrafiltrate, obtains refined solution.
7. the preparation method of mushroom with abundant selenium protein peptide powder as claimed in claim 6, which is characterized in that described to use retention molecule
In the step of ultrafiltration membrane that amount is 5kDa~10kDa carries out constant volume ultrafiltration to the mixed liquor, obtains ultrafiltrate, the constant volume
The condition of ultrafiltration are as follows: ultrafiltration pressure is 0.16MPa~0.19MPa, 20~25 DEG C of temperature.
8. the preparation method of mushroom with abundant selenium protein peptide powder as claimed in claim 6, which is characterized in that pass through the ultrafiltrate
In the step of molecular cut off is the nanofiltration membrane constant volume nanofiltration of 1kDa~3kDa, obtains refined solution, the item of the constant volume nanofiltration
Part are as follows: pressure is 1.9MPa~2.1MPa, 20~25 DEG C of temperature.
9. a kind of selenium-rich solid beverage, which is characterized in that the component including following parts by weight: such as any one of claim 1 to 8
600~800 parts of selenium-enriched protein peptide made from the preparation method of the mushroom with abundant selenium protein peptide powder, 60~120 parts of soybean milk powder, mountain
It is 5~20 parts of medicine polysaccharide, 20~30 parts of red date powder, 15~30 parts of Cordyceps militaris powder, 90~180 parts of white granulated sugar, 9~18 parts of salt, big
1~2 part of Fabaceous Lecithin and 0.04~0.05 part of vitamin E.
10. selenium-rich solid beverage as claimed in claim 9, which is characterized in that the selenium-rich solid beverage is tablet or pulvis.
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| CN113951367A (en) * | 2021-11-16 | 2022-01-21 | 安徽中志科技有限公司 | Edible fungus protein powder with high organic selenium content, preparation method and application |
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