CN110373152B - 一种高强度的稀土蛋白粘合剂及其制备方法 - Google Patents
一种高强度的稀土蛋白粘合剂及其制备方法 Download PDFInfo
- Publication number
- CN110373152B CN110373152B CN201910777870.2A CN201910777870A CN110373152B CN 110373152 B CN110373152 B CN 110373152B CN 201910777870 A CN201910777870 A CN 201910777870A CN 110373152 B CN110373152 B CN 110373152B
- Authority
- CN
- China
- Prior art keywords
- protein
- adhesive
- anionic surfactant
- positive charge
- super
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 106
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 106
- 230000001070 adhesive effect Effects 0.000 title claims abstract description 79
- 239000000853 adhesive Substances 0.000 title claims abstract description 76
- 229910052761 rare earth metal Inorganic materials 0.000 title abstract description 40
- 150000002910 rare earth metals Chemical class 0.000 title abstract description 40
- 238000002360 preparation method Methods 0.000 title abstract description 15
- 239000003945 anionic surfactant Substances 0.000 claims abstract description 44
- YCIMNLLNPGFGHC-UHFFFAOYSA-N catechol Chemical group OC1=CC=CC=C1O YCIMNLLNPGFGHC-UHFFFAOYSA-N 0.000 claims abstract description 18
- 229910021645 metal ion Inorganic materials 0.000 claims abstract description 17
- 239000000243 solution Substances 0.000 claims description 28
- 239000011230 binding agent Substances 0.000 claims description 12
- 241001052560 Thallis Species 0.000 claims description 8
- 230000014509 gene expression Effects 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- 239000012460 protein solution Substances 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 238000005119 centrifugation Methods 0.000 claims description 7
- 238000004128 high performance liquid chromatography Methods 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 6
- 238000004108 freeze drying Methods 0.000 claims description 5
- 102000004190 Enzymes Human genes 0.000 claims description 4
- 108090000790 Enzymes Proteins 0.000 claims description 4
- 241000588724 Escherichia coli Species 0.000 claims description 4
- 102000016943 Muramidase Human genes 0.000 claims description 4
- 108010014251 Muramidase Proteins 0.000 claims description 4
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 claims description 4
- 229940124158 Protease/peptidase inhibitor Drugs 0.000 claims description 4
- 230000001580 bacterial effect Effects 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 4
- 229940088598 enzyme Drugs 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 4
- 239000000411 inducer Substances 0.000 claims description 4
- 230000001939 inductive effect Effects 0.000 claims description 4
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 claims description 4
- 239000012139 lysis buffer Substances 0.000 claims description 4
- 229960000274 lysozyme Drugs 0.000 claims description 4
- 239000004325 lysozyme Substances 0.000 claims description 4
- 235000010335 lysozyme Nutrition 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 230000002018 overexpression Effects 0.000 claims description 4
- 239000000137 peptide hydrolase inhibitor Substances 0.000 claims description 4
- 239000013612 plasmid Substances 0.000 claims description 4
- QLNJFJADRCOGBJ-UHFFFAOYSA-N propionamide Chemical compound CCC(N)=O QLNJFJADRCOGBJ-UHFFFAOYSA-N 0.000 claims description 4
- 229940080818 propionamide Drugs 0.000 claims description 4
- 108010071967 protein K Proteins 0.000 claims description 4
- 230000001131 transforming effect Effects 0.000 claims description 4
- 229910021642 ultra pure water Inorganic materials 0.000 claims description 4
- 239000012498 ultrapure water Substances 0.000 claims description 4
- 241000235058 Komagataella pastoris Species 0.000 claims description 3
- NKKSCKHNGIWSDQ-UHFFFAOYSA-N S(=O)(=O)(O)O.C(CCCCC)[Na] Chemical compound S(=O)(=O)(O)O.C(CCCCC)[Na] NKKSCKHNGIWSDQ-UHFFFAOYSA-N 0.000 claims description 3
- 241001465754 Metazoa Species 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 4
- 239000013522 chelant Substances 0.000 abstract description 3
- 230000002708 enhancing effect Effects 0.000 abstract description 3
- 210000002615 epidermis Anatomy 0.000 abstract description 3
- 150000001450 anions Chemical class 0.000 abstract description 2
- 150000001875 compounds Chemical class 0.000 abstract description 2
- 239000007864 aqueous solution Substances 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 239000004094 surface-active agent Substances 0.000 description 5
- 229910000831 Steel Inorganic materials 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 239000010959 steel Substances 0.000 description 4
- 239000003292 glue Substances 0.000 description 3
- 210000003491 skin Anatomy 0.000 description 3
- 229910021578 Iron(III) chloride Inorganic materials 0.000 description 2
- 239000004698 Polyethylene Substances 0.000 description 2
- 150000001217 Terbium Chemical class 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000005034 decoration Methods 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- -1 polyethylene Polymers 0.000 description 2
- 229920000573 polyethylene Polymers 0.000 description 2
- 229920000915 polyvinyl chloride Polymers 0.000 description 2
- 239000004800 polyvinyl chloride Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- DZAUWHJDUNRCTF-UHFFFAOYSA-N 3-(3,4-dihydroxyphenyl)propanoic acid Chemical compound OC(=O)CCC1=CC=C(O)C(O)=C1 DZAUWHJDUNRCTF-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 230000010062 adhesion mechanism Effects 0.000 description 1
- 238000005576 amination reaction Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000009920 chelation Effects 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000010511 deprotection reaction Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- IDUWTCGPAPTSFB-UHFFFAOYSA-N hexyl hydrogen sulfate Chemical compound CCCCCCOS(O)(=O)=O IDUWTCGPAPTSFB-UHFFFAOYSA-N 0.000 description 1
- 238000000703 high-speed centrifugation Methods 0.000 description 1
- 150000002500 ions Chemical group 0.000 description 1
- 150000002505 iron Chemical class 0.000 description 1
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical group Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 description 1
- 239000012567 medical material Substances 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 238000010534 nucleophilic substitution reaction Methods 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- GFISHBQNVWAVFU-UHFFFAOYSA-K terbium(iii) chloride Chemical group Cl[Tb](Cl)Cl GFISHBQNVWAVFU-UHFFFAOYSA-K 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09J—ADHESIVES; NON-MECHANICAL ASPECTS OF ADHESIVE PROCESSES IN GENERAL; ADHESIVE PROCESSES NOT PROVIDED FOR ELSEWHERE; USE OF MATERIALS AS ADHESIVES
- C09J11/00—Features of adhesives not provided for in group C09J9/00, e.g. additives
- C09J11/02—Non-macromolecular additives
- C09J11/06—Non-macromolecular additives organic
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09J—ADHESIVES; NON-MECHANICAL ASPECTS OF ADHESIVE PROCESSES IN GENERAL; ADHESIVE PROCESSES NOT PROVIDED FOR ELSEWHERE; USE OF MATERIALS AS ADHESIVES
- C09J189/00—Adhesives based on proteins; Adhesives based on derivatives thereof
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Materials For Medical Uses (AREA)
- Adhesives Or Adhesive Processes (AREA)
Abstract
Description
Claims (6)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910777870.2A CN110373152B (zh) | 2019-08-22 | 2019-08-22 | 一种高强度的稀土蛋白粘合剂及其制备方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910777870.2A CN110373152B (zh) | 2019-08-22 | 2019-08-22 | 一种高强度的稀土蛋白粘合剂及其制备方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN110373152A CN110373152A (zh) | 2019-10-25 |
CN110373152B true CN110373152B (zh) | 2021-08-17 |
Family
ID=68260304
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201910777870.2A Active CN110373152B (zh) | 2019-08-22 | 2019-08-22 | 一种高强度的稀土蛋白粘合剂及其制备方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN110373152B (zh) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114292621B (zh) * | 2021-12-29 | 2023-05-02 | 清华大学 | 一种基于生物工程蛋白的高低温粘合剂及其制备方法 |
CN114712330A (zh) * | 2022-01-18 | 2022-07-08 | 中国科学院长春应用化学研究所 | 一种生物工程蛋白膏药及其制备方法与应用 |
CN116496415B (zh) * | 2023-04-27 | 2024-04-23 | 北京镧系生物科技有限公司 | 一种模块化蛋白、包含其的粘合剂及其制备方法和应用 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2009021287A1 (en) * | 2007-08-14 | 2009-02-19 | Commonwealth Scientific And Industrial Research Organisation | Photoactivated crosslinking of a protein or peptide |
JP5530934B2 (ja) * | 2008-01-24 | 2014-06-25 | ユニバーシティ オブ ユタ リサーチ ファンデーション | 接着複合コアセルベートならびにその作製および使用方法 |
AU2012275516A1 (en) * | 2011-06-28 | 2014-01-23 | University Of Utah Research Foundation | Adhesive complex coacervates and methods of making and using thereof |
CN103965810B (zh) * | 2014-04-11 | 2016-04-20 | 中国科学院青岛生物能源与过程研究所 | 一种可在潮湿表面粘接及水下固化的仿贻贝胶的制备方法 |
CN106636016B (zh) * | 2017-02-24 | 2020-03-17 | 天津大学 | 一种通过正负电荷引入辅助病毒样颗粒自组装的方法和应用 |
KR102010051B1 (ko) * | 2017-09-20 | 2019-08-12 | 주식회사 포스코 | 카테콜 치환된 음이온성 고분자로부터 형성된 코아세르베이트, 이를 포함하는 접착제 및 이의 제조방법 |
-
2019
- 2019-08-22 CN CN201910777870.2A patent/CN110373152B/zh active Active
Also Published As
Publication number | Publication date |
---|---|
CN110373152A (zh) | 2019-10-25 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN110373152B (zh) | 一种高强度的稀土蛋白粘合剂及其制备方法 | |
Lee et al. | VATA: a poly (vinyl alcohol)-and tannic acid-based nontoxic underwater adhesive | |
CN110452662B (zh) | 一种基于生物工程蛋白的生物粘合剂及其制备方法 | |
Zhao et al. | Mussel-mimetic hydrogels with defined cross-linkers achieved via controlled catechol dimerization exhibiting tough adhesion for wet biological tissues | |
McGann et al. | Resilin‐based hybrid hydrogels for cardiovascular tissue engineering | |
Katakura et al. | Lactic acid bacteria display on the cell surface cytosolic proteins that recognize yeast mannan | |
Choi et al. | In vivo modification of tyrosine residues in recombinant mussel adhesive protein by tyrosinase co-expression in Escherichia coli | |
Cuatrecasas | Agarose derivatives for purification of protein by affinity chromatography | |
Cha et al. | Bulk adhesive strength of recombinant hybrid mussel adhesive protein | |
Zhang et al. | Recent Progress of Mussel‐Inspired Underwater Adhesives | |
CN110373153B (zh) | 一种高粘合强度的生物粘合剂及其制备方法 | |
Behra et al. | Magnetic porous sugar-functionalized PEG microgels for efficient isolation and removal of bacteria from solution | |
MX2011003502A (es) | Anticuerpo de cadena sencilla biespecifica de psmaxcd3 especifico a traves de especies. | |
US11046873B2 (en) | Methods of adhering in an aqueous environment | |
CN106496438A (zh) | 一种酰胺化氧化石墨烯改性聚羧酸减水剂及其制备方法 | |
WO2022111661A1 (zh) | 一种抗体型生物磁性微球及其制备方法和应用 | |
JPWO2011125673A1 (ja) | アフィニティークロマトグラフィー用充填剤 | |
DE60019589D1 (de) | Schnelle dehydrierung von proteinen | |
Ryoyama | Studies on the biological properties of coelomic fluid of sea urchin 1. Naturally occurring hemolysin in sea urchin | |
Li et al. | Antifouling and antimicrobial cobaltocenium-containing metallopolymer double-network hydrogels | |
CN110484990B (zh) | 一种稀土蛋白纤维及其制备方法 | |
Bolghari et al. | A novel recombinant chimeric bio-adhesive protein consisting of mussel foot protein 3, 5, gas vesicle protein A, and CsgA curli protein expressed in Pichia pastoris | |
JP2003528175A (ja) | 大腸菌からのリポ多糖 | |
CN112625650B (zh) | 一种明胶基水凝胶粘合剂及其制备方法和应用 | |
JP2019073673A (ja) | 自己修復性ゲル |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20230323 Address after: Room 409-339, Floor 4, Building 1, No. 38, Yongda Road, Daxing Biomedical Industry Base, Zhongguancun Science Park, Daxing District, Beijing, 102629 (cluster registration) Patentee after: Beijing Lanthanide Biotechnology Co.,Ltd. Address before: 130022 No. 5625 Renmin Street, Jilin, Changchun Patentee before: CHANGCHUN INSTITUTE OF APPLIED CHEMISTRY CHINESE ACADEMY OF SCIENCES |
|
TR01 | Transfer of patent right |
Effective date of registration: 20240611 Address after: 215124, Floor 1703, Building 5, Tianyun Square, Guoxiang Street, Wuzhong District, Suzhou City, Jiangsu Province Patentee after: Suzhou Kangchengyuan Technology Partnership Enterprise (Limited Partnership) Country or region after: China Address before: Room 409-339, Floor 4, Building 1, No. 38, Yongda Road, Daxing Biomedical Industry Base, Zhongguancun Science Park, Daxing District, Beijing, 102629 (cluster registration) Patentee before: Beijing Lanthanide Biotechnology Co.,Ltd. Country or region before: China |