CN110368523A - A kind of method of the bone filling bracket of 3D printing PEI surface recombination RGD - Google Patents
A kind of method of the bone filling bracket of 3D printing PEI surface recombination RGD Download PDFInfo
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- CN110368523A CN110368523A CN201910507236.7A CN201910507236A CN110368523A CN 110368523 A CN110368523 A CN 110368523A CN 201910507236 A CN201910507236 A CN 201910507236A CN 110368523 A CN110368523 A CN 110368523A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/227—Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/28—Materials for coating prostheses
- A61L27/34—Macromolecular materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y10/00—Processes of additive manufacturing
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J7/00—Chemical treatment or coating of shaped articles made of macromolecular substances
- C08J7/12—Chemical modification
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J7/00—Chemical treatment or coating of shaped articles made of macromolecular substances
- C08J7/12—Chemical modification
- C08J7/16—Chemical modification with polymerisable compounds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/20—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing organic materials
- A61L2300/252—Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
- A61L2300/606—Coatings
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/18—Modification of implant surfaces in order to improve biocompatibility, cell growth, fixation of biomolecules, e.g. plasma treatment
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2420/00—Materials or methods for coatings medical devices
- A61L2420/02—Methods for coating medical devices
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2420/00—Materials or methods for coatings medical devices
- A61L2420/08—Coatings comprising two or more layers
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2379/00—Characterised by the use of macromolecular compounds obtained by reactions forming in the main chain of the macromolecule a linkage containing nitrogen with or without oxygen, or carbon only, not provided for in groups C08J2361/00 - C08J2377/00
- C08J2379/04—Polycondensates having nitrogen-containing heterocyclic rings in the main chain; Polyhydrazides; Polyamide acids or similar polyimide precursors
- C08J2379/08—Polyimides; Polyester-imides; Polyamide-imides; Polyamide acids or similar polyimide precursors
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2479/00—Characterised by the use of macromolecular compounds obtained by reactions forming in the main chain of the macromolecule a linkage containing nitrogen with or without oxygen, or carbon only, not provided for in groups C08J2461/00 - C08J2477/00
- C08J2479/04—Polycondensates having nitrogen-containing heterocyclic rings in the main chain; Polyhydrazides; Polyamide acids or similar polyimide precursors
Abstract
The invention discloses a kind of methods of the bone filling bracket of 3D printing PEI surface recombination RGD, choose PEI particle, dry, be then placed in wire drawing machine cartridge in air circulating oven, PEI is squeezed into filiform；According to the shape of required bone filling bracket, the supporting structure model of pre-print is established using 3 d modeling software, and being translated into 3D printing system can be with identified stl file；PEI silk material is sent into the printing head being preheated by automatic wire feeding device, 3D printing spray head is successively printed according to the trajectory path of Software Create, until printing is completed to obtain PEI bracket；PEI bracket is immersed in the dopamine solution that concentration is 2mg/ml, then in being soaked in the rgd peptide solution that concentration is 200 μ g/ml, the PEI bracket that can promote bone tissue reparation is made.PEI bracket in the present invention can promote cell adhesion, promote Osteoblast Differentiation, be conducive to bone tissue reparation.
Description
Technical field
The present invention relates to biomedical engineering technology field, more particularly to a kind of 3D printing PEI surface recombination
The method of the bone filling bracket of RGD.
Background technique
Since wound, tumor resection, operation etc. will lead to the impaired position bulk bone defect of human body, it need to often carry out bone grafting and repair
It is multiple.Traditional bone collection method includes autologous bone transplanting and two kinds of allogenic bone transplantation.Autologous bone transplanting generally uses patient itself
Bone is transplanted, although the method is immunoreacted lower, limited source, and new wound can be caused for bone area to human body
Wound；Allogenic bone transplantation is not limited by size shape etc., but can cause stronger immune response, and healing time is also relatively slow, and holds
Cross-infection easily occurs.
Tissue engineering bracket is used gradually in recent years, instead of self bone and allosome bone, is solved to a certain extent
The drawbacks of above-mentioned bone collection method of having determined, and 3D printing technique can be largely fulfilled the porosity of bracket, aperture, hole
The controllability of volume, space arrangement and other surfaces characteristic, it is thus possible to realize the preparation of excellent bone tissue engineering scaffold.But
The function of preparing resulting bone repairing support by 3D printing at present is more single, and cell adhesion can be promoted by not having, be conducive to
The effect of bone tissue reparation.
Therefore, a kind of bone filling bracket that can promote bone tissue reparation is developed, is that those skilled in the art need to solve
Certainly the problem of.
Summary of the invention
In view of this, the present invention provides one kind can promote cell adhesion, promotes Osteoblast Differentiation, be conducive to bone tissue reparation
3D printing PEI surface recombination RGD bone filling bracket method.
To achieve the goals above, the present invention adopts the following technical scheme:
A kind of method of the bone filling bracket of 3D printing PEI surface recombination RGD, including following preparation step:
Step 1: PEI particle is chosen, PEI particle is placed in 140 DEG C -180 DEG C of air circulating oven and is dried 3-5 hours,
Making it, sufficiently drying can stop until when moisture is less than 0.02%w/w, the particle after drying is put into wire drawing machine cartridge,
Wire drawing machine cartridge is heated, until temperature reaches 340-420 DEG C of stopping, being melted completely to the PEI particle in wire drawing machine cartridge
It after change, is squeezed, PEI is squeezed into filiform, the PEI silk squeezed again passes by 140 DEG C -180 DEG C of air circulation
Furnace dries 3-5 hours, obtains dry PEI silk, be then sealed preservation for PEI；
Step 2: according to the shape of required bone filling bracket, the branch of pre-print modeling: is established using 3 d modeling software
Frame structural model, and being translated into 3D printing system can be with identified stl file；
Step 3: stl file obtained in the step 2 being imported into 3D printer system, automatic temperature control system is passed through
3D printer spray head, Photocopy Room, shaping substrate are preheated, are then sent into PEI silk material by automatic wire feeding device pre-
In the good printing head of heat, 3D printing spray head is successively printed according to the trajectory path of Software Create, until printing is completed
To PEI bracket, then obtained PEI bracket is put into toast 15-30 minutes in 100-150 DEG C of oven and can be completed；
Step 4: PEI bracket obtained in the step 3 being immersed in the dopamine solution that concentration is 2mg/ml, 37
DEG C constant temperature and processing 12-24 hour is protected from light under conditions of coming into full contact with air, obtain PEI branch of the surface through dopamine modification
Then frame carries out after rinsing 2-3 times repeatedly modified PEI bracket with deionized water, then carries out freeze-drying preservation；
Step 5: the rgd peptide that concentration is 200 μ g/ml will be soaked in through the amine-modified PEI bracket of DOPA in the step 4
In solution, it is placed in taking-up PEI bracket after handling 12-24 hours on 37 DEG C of constant-temperature tables, then uses deionized water repeated flushing
Remove the unbonded securely remaining rgd peptide of PEI rack surface, save backup the freeze-drying of PEI bracket after handle, it is obtained can be with
Promote the PEI bracket of bone tissue reparation.
In above-mentioned preparation step after the completion of the printing of PEI bracket, PEI bracket is toasted, it is ensured that on PEI bracket
Can preferably it stick between different layers, so that the globality of PEI bracket is more preferable.Dopamine in dopamine solution can be spontaneous poly-
Collection reaction forms poly-dopamine, and the surface adhesion for having impregnated the PEI bracket of dopamine solution has a poly-dopamine, then by PEI bracket
Be dipped into rgd peptide solution, in rgd peptide contain carboxyl and amino isoreactivity group, can with it is abundant in poly-dopamine layer
Conjugated occurs for catechol group, to be grafted coupling rgd peptide in poly-dopamine layer, rgd peptide can promote cell
Stick, promote Osteoblast Differentiation, be conducive to bone tissue reparation, the 3D printing PEI bone filling bracket of the grafting coupling RGD of preparation can promote
Into bone tissue reparation, so that filling bracket and bone tissue be made to be implemented in combination with the effect that bone defect healing is filled.
Preferably, it is waited 3-5 seconds after the temperature of wire drawing machine cartridge reaches 340-420 DEG C in the step 1, to PEI
After grain melts completely, then PEI is squeezed.
Preferably, the internal diameter of the extrusion of wire drawing machine cartridge is 1.5-2.0mm in the step 1.Obtained by can guaranteeing
PEI silk diameter within the scope of 1.5-2.0mm.
Preferably, in the step 1 when being sealed preservation to PEI, 2-3 packet silicon is put into the device that is sealed
Glue desiccant.Silica-gel desiccant can prevent dry PEI silk absorption moisture from having an impact to 3D printing.
Preferably, 3D printer spray head is preheating to 360-415 DEG C by automatic temperature control system in the step 3, and printer is beaten
Print room is preheating to 210-230 DEG C, and shaping substrate is preheating to 110-180 DEG C.
Preferably, it is 10mmol/L in concentration, dopamine is added in the Tris-HCl buffer of PH=8.5, is made described
Concentration is the dopamine solution of 2mg/ml in step 4.
Preferably, it is 10mmol/L in concentration, rgd peptide is added in the Tris-HCl buffer of PH=8.5, is made described
Concentration is the rgd peptide solution of 200 μ g/ml in step 5.
Beneficial effects of the present invention:
(1) the bone filling bracket of required shape can be printed by 3D printing technique, prints the PEI bracket of completion
It can make to stick between the different layers of PEI bracket after drying more preferable, keep the integrality of PEI bracket, stability more preferable；
(2) PEI bracket is successively soaked in dopamine solution and rgd peptide solution, the dopamine meeting in dopamine solution
Self-assemble reacts to form poly-dopamine, in rgd peptide contain carboxyl and amino isoreactivity group, can with it is rich in poly-dopamine layer
Conjugated occurs for rich catechol group, to be grafted coupling rgd peptide in poly-dopamine layer, rgd peptide can promote
Cell adhesion promotes Osteoblast Differentiation, is conducive to bone tissue reparation, allows the PEI bracket of preparation to promote bone tissue reparation, in turn
Filling bracket and bone tissue is set to be implemented in combination with the effect of bone defect healing filling.
Specific embodiment
The following is a clear and complete description of the technical scheme in the embodiments of the invention, it is clear that described embodiment
Only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, the common skill in this field
Art personnel every other embodiment obtained without making creative work belongs to the model that the present invention protects
It encloses.
The embodiment of the invention discloses a kind of method of the bone filling bracket of 3D printing PEI surface recombination RGD, including it is as follows
Preparation step:
Step 1: PEI particle is chosen, PEI particle is placed in 140 DEG C -180 DEG C of air circulating oven and is dried 3-5 hours,
Making it, sufficiently drying can stop until when moisture is less than 0.02%w/w, the particle after drying is put into wire drawing machine cartridge,
Wire drawing machine cartridge is heated, until temperature reaches 340-420 DEG C of stopping, being waited 3-5 seconds, to the PEI in wire drawing machine cartridge
It after particle melts completely, is squeezed, the internal diameter of the extrusion of wire drawing machine cartridge is 1.5-2.0mm, and PEI is squeezed into filiform
Object, the PEI silk squeezed again pass by 140 DEG C -180 DEG C of air circulating oven, dry 3-5 hours, obtain dry PEI
Silk, is then sealed preservation for PEI, 2-3 packet silica-gel desiccant is put into the device being sealed；
Step 2: according to the shape of required bone filling bracket, the branch of pre-print modeling: is established using 3 d modeling software
Frame structural model, and being translated into 3D printing system can be with identified stl file；
Step 3: stl file obtained in step 2 being imported into 3D printer system, by automatic temperature control system by 3D
Printer head is preheating to 360-415 DEG C, and printer Photocopy Room is preheating to 210-230 DEG C, and shaping substrate is preheating to 110-180
DEG C, then PEI silk material is sent into the printing head being preheated by automatic wire feeding device, 3D printing spray head is raw according to software
At trajectory path successively printed, until printing is completed to obtain PEI bracket, then obtained PEI bracket is put into 100-150
DEG C oven in baking can be completed within 15-30 minutes；
Step 4: being 10mmol/L in concentration, dopamine is added in the Tris-HCl buffer of PH=8.5, obtained concentration is
PEI bracket obtained in step 3 is immersed in the dopamine solution that concentration is 2mg/ml by the dopamine solution of 2mg/ml,
37 DEG C of constant temperature and it is protected from light processing 12-24 hour under conditions of coming into full contact with air, obtains PEI branch of the surface through dopamine modification
Then frame carries out after rinsing 2-3 times repeatedly modified PEI bracket with deionized water, then carries out freeze-drying preservation；
Step 5: being 10mmol/L in concentration, rgd peptide is added in the Tris-HCl buffer of PH=8.5, concentration is made
It is 200 μ g/ml's by concentration is soaked in through the amine-modified PEI bracket of DOPA in step 4 for the rgd peptide solution of 200 μ g/ml
In rgd peptide solution, it is placed in taking-up PEI bracket after handling 12-24 hours on 37 DEG C of constant-temperature tables, it is then anti-with deionized water
The rgd peptide for washing off the unbonded securely remnants of PEI rack surface again, the freeze-drying of PEI bracket is saved backup, make after having handled
Obtain the PEI bracket that can promote bone tissue reparation.
Embodiment 1:
Step 1: choosing PEI particle, PEI particle is placed in 140 DEG C of air circulating oven and is dried 5 hours, make it sufficiently
Drying can stop, the particle after drying being put into wire drawing machine cartridge, to wire drawing machine until when moisture is less than 0.02%w/w
Barrel is heated, until temperature reaches 340 DEG C of stoppings, being waited 5 seconds, after the PEI particle in wire drawing machine cartridge melts completely,
It is squeezed, the internal diameter of the extrusion of wire drawing machine cartridge is 1.5mm, PEI is squeezed into filiform, the PEI silk squeezed is again
The secondary air circulating oven for passing through 140 DEG C, dries 5 hours, obtains dry PEI silk, be then sealed preservation for PEI,
2 packet silica-gel desiccants are put into the device being sealed；
Step 2: according to the shape of required bone filling bracket, the branch of pre-print modeling: is established using 3 d modeling software
Frame structural model, and being translated into 3D printing system can be with identified stl file；
Step 3: stl file obtained in step 2 being imported into 3D printer system, by automatic temperature control system by 3D
Printer head is preheating to 360 DEG C, and printer Photocopy Room is preheating to 210 DEG C, and shaping substrate is preheating to 110 DEG C, then by certainly
PEI silk material is sent into the printing head being preheated by dynamic wire feeder, and 3D printing spray head is according to the trajectory path of Software Create
It is successively printed, until printing is completed to obtain PEI bracket, then obtained PEI bracket is put into 100 DEG C of oven and toasts 30
Minute can be completed；
Step 4: being 10mmol/L in concentration, dopamine is added in the Tris-HCl buffer of PH=8.5, obtained concentration is
PEI bracket obtained in step 3 is immersed in the dopamine solution that concentration is 2mg/ml by the dopamine solution of 2mg/ml,
37 DEG C of constant temperature and it is protected from light processing 12 hours under conditions of coming into full contact with air, obtains the surface PEI bracket modified through dopamine,
Then modified PEI bracket is carried out after rinsing 2 times repeatedly with deionized water, then carries out freeze-drying preservation；
Step 5: being 10mmol/L in concentration, rgd peptide is added in the Tris-HCl buffer of PH=8.5, concentration is made
It is 200 μ g/ml's by concentration is soaked in through the amine-modified PEI bracket of DOPA in step 4 for the rgd peptide solution of 200 μ g/ml
In rgd peptide solution, it is placed in taking-up PEI bracket after handling 12 hours on 37 DEG C of constant-temperature tables, then repeatedly with deionized water
The rgd peptide for washing off the unbonded securely remnants of PEI rack surface, the freeze-drying of PEI bracket is saved backup, be made after having handled
It can promote the PEI bracket of bone tissue reparation.
Embodiment 2:
Step 1: choosing PEI particle, PEI particle is placed in 150 DEG C of air circulating oven and is dried 5 hours, make it sufficiently
Drying can stop, the particle after drying being put into wire drawing machine cartridge, to wire drawing machine until when moisture is less than 0.02%w/w
Barrel is heated, until temperature reaches 360 DEG C of stoppings, being waited 5 seconds, after the PEI particle in wire drawing machine cartridge melts completely,
It is squeezed, the internal diameter of the extrusion of wire drawing machine cartridge is 1.6mm, PEI is squeezed into filiform, the PEI silk squeezed is again
The secondary air circulating oven for passing through 150 DEG C, dries 5 hours, obtains dry PEI silk, be then sealed preservation for PEI,
2 packet silica-gel desiccants are put into the device being sealed；
Step 2: according to the shape of required bone filling bracket, the branch of pre-print modeling: is established using 3 d modeling software
Frame structural model, and being translated into 3D printing system can be with identified stl file；
Step 3: stl file obtained in step 2 being imported into 3D printer system, by automatic temperature control system by 3D
Printer head is preheating to 380 DEG C, and printer Photocopy Room is preheating to 210 DEG C, and shaping substrate is preheating to 130 DEG C, then by certainly
PEI silk material is sent into the printing head being preheated by dynamic wire feeder, and 3D printing spray head is according to the trajectory path of Software Create
It is successively printed, until printing is completed to obtain PEI bracket, then obtained PEI bracket is put into 110 DEG C of oven and toasts 28
Minute can be completed；
Step 4: being 10mmol/L in concentration, dopamine is added in the Tris-HCl buffer of PH=8.5, obtained concentration is
PEI bracket obtained in step 3 is immersed in the dopamine solution that concentration is 2mg/ml by the dopamine solution of 2mg/ml,
37 DEG C of constant temperature and it is protected from light processing 15 hours under conditions of coming into full contact with air, obtains the surface PEI bracket modified through dopamine,
Then modified PEI bracket is carried out after rinsing 2 times repeatedly with deionized water, then carries out freeze-drying preservation；
Step 5: being 10mmol/L in concentration, rgd peptide is added in the Tris-HCl buffer of PH=8.5, concentration is made
It is 200 μ g/ml's by concentration is soaked in through the amine-modified PEI bracket of DOPA in step 4 for the rgd peptide solution of 200 μ g/ml
In rgd peptide solution, it is placed in taking-up PEI bracket after handling 15 hours on 37 DEG C of constant-temperature tables, then repeatedly with deionized water
The rgd peptide for washing off the unbonded securely remnants of PEI rack surface, the freeze-drying of PEI bracket is saved backup, be made after having handled
It can promote the PEI bracket of bone tissue reparation.
Embodiment 3:
Step 1: choosing PEI particle, PEI particle is placed in 160 DEG C of air circulating oven and is dried 4 hours, make it sufficiently
Drying can stop, the particle after drying being put into wire drawing machine cartridge, to wire drawing machine until when moisture is less than 0.02%w/w
Barrel is heated, until temperature reaches 380 DEG C of stoppings, being waited 4 seconds, after the PEI particle in wire drawing machine cartridge melts completely,
It is squeezed, the internal diameter of the extrusion of wire drawing machine cartridge is 1.7mm, PEI is squeezed into filiform, the PEI silk squeezed is again
The secondary air circulating oven for passing through 160 DEG C, dries 4 hours, obtains dry PEI silk, be then sealed preservation for PEI,
3 packet silica-gel desiccants are put into the device being sealed；
Step 2: according to the shape of required bone filling bracket, the branch of pre-print modeling: is established using 3 d modeling software
Frame structural model, and being translated into 3D printing system can be with identified stl file；
Step 3: stl file obtained in step 2 being imported into 3D printer system, by automatic temperature control system by 3D
Printer head is preheating to 400 DEG C, and printer Photocopy Room is preheating to 220 DEG C, and shaping substrate is preheating to 140 DEG C, then by certainly
PEI silk material is sent into the printing head being preheated by dynamic wire feeder, and 3D printing spray head is according to the trajectory path of Software Create
It is successively printed, until printing is completed to obtain PEI bracket, then obtained PEI bracket is put into 130 DEG C of oven and toasts 25
Minute can be completed；
Step 4: being 10mmol/L in concentration, dopamine is added in the Tris-HCl buffer of PH=8.5, obtained concentration is
PEI bracket obtained in step 3 is immersed in the dopamine solution that concentration is 2mg/ml by the dopamine solution of 2mg/ml,
37 DEG C of constant temperature and it is protected from light processing 18 hours under conditions of coming into full contact with air, obtains the surface PEI bracket modified through dopamine,
Then modified PEI bracket is carried out after rinsing 3 times repeatedly with deionized water, then carries out freeze-drying preservation；
Step 5: being 10mmol/L in concentration, rgd peptide is added in the Tris-HCl buffer of PH=8.5, concentration is made
It is 200 μ g/ml's by concentration is soaked in through the amine-modified PEI bracket of DOPA in step 4 for the rgd peptide solution of 200 μ g/ml
In rgd peptide solution, it is placed in taking-up PEI bracket after handling 18 hours on 37 DEG C of constant-temperature tables, then repeatedly with deionized water
The rgd peptide for washing off the unbonded securely remnants of PEI rack surface, the freeze-drying of PEI bracket is saved backup, be made after having handled
It can promote the PEI bracket of bone tissue reparation.
Embodiment 4:
Step 1: choosing PEI particle, PEI particle is placed in 170 DEG C of air circulating oven and is dried 4 hours, make it sufficiently
Drying can stop, the particle after drying being put into wire drawing machine cartridge, to wire drawing machine until when moisture is less than 0.02%w/w
Barrel is heated, until temperature reaches 400 DEG C of stoppings, being waited 4 seconds, after the PEI particle in wire drawing machine cartridge melts completely,
It is squeezed, the internal diameter of the extrusion of wire drawing machine cartridge is 1.8mm, PEI is squeezed into filiform, the PEI silk squeezed is again
The secondary air circulating oven for passing through 170 DEG C, dries 4 hours, obtains dry PEI silk, be then sealed preservation for PEI,
3 packet silica-gel desiccants are put into the device being sealed；
Step 2: according to the shape of required bone filling bracket, the branch of pre-print modeling: is established using 3 d modeling software
Frame structural model, and being translated into 3D printing system can be with identified stl file；
Step 3: stl file obtained in step 2 being imported into 3D printer system, by automatic temperature control system by 3D
Printer head is preheating to 410 DEG C, and printer Photocopy Room is preheating to 230 DEG C, and shaping substrate is preheating to 160 DEG C, then by certainly
PEI silk material is sent into the printing head being preheated by dynamic wire feeder, and 3D printing spray head is according to the trajectory path of Software Create
It is successively printed, until printing is completed to obtain PEI bracket, then obtained PEI bracket is put into 140 DEG C of oven and toasts 20
Minute can be completed；
Step 4: being 10mmol/L in concentration, dopamine is added in the Tris-HCl buffer of PH=8.5, obtained concentration is
PEI bracket obtained in step 3 is immersed in the dopamine solution that concentration is 2mg/ml by the dopamine solution of 2mg/ml,
37 DEG C of constant temperature and it is protected from light processing 21 hours under conditions of coming into full contact with air, obtains the surface PEI bracket modified through dopamine,
Then modified PEI bracket is carried out after rinsing 3 times repeatedly with deionized water, then carries out freeze-drying preservation；
Step 5: being 10mmol/L in concentration, rgd peptide is added in the Tris-HCl buffer of PH=8.5, concentration is made
It is 200 μ g/ml's by concentration is soaked in through the amine-modified PEI bracket of DOPA in step 4 for the rgd peptide solution of 200 μ g/ml
In rgd peptide solution, it is placed in taking-up PEI bracket after handling 21 hours on 37 DEG C of constant-temperature tables, then repeatedly with deionized water
The rgd peptide for washing off the unbonded securely remnants of PEI rack surface, the freeze-drying of PEI bracket is saved backup, be made after having handled
It can promote the PEI bracket of bone tissue reparation.
Embodiment 5:
Step 1: choosing PEI particle, PEI particle is placed in 180 DEG C of air circulating oven and is dried 3 hours, make it sufficiently
Drying can stop, the particle after drying being put into wire drawing machine cartridge, to wire drawing machine until when moisture is less than 0.02%w/w
Barrel is heated, until temperature reaches 420 DEG C of stoppings, being waited 3 seconds, after the PEI particle in wire drawing machine cartridge melts completely,
It is squeezed, the internal diameter of the extrusion of wire drawing machine cartridge is 2.0mm, PEI is squeezed into filiform, the PEI silk squeezed is again
The secondary air circulating oven for passing through 180 DEG C, dries 3 hours, obtains dry PEI silk, be then sealed preservation for PEI,
3 packet silica-gel desiccants are put into the device being sealed；
Step 2: according to the shape of required bone filling bracket, the branch of pre-print modeling: is established using 3 d modeling software
Frame structural model, and being translated into 3D printing system can be with identified stl file；
Step 3: stl file obtained in step 2 being imported into 3D printer system, by automatic temperature control system by 3D
Printer head is preheating to 415 DEG C, and printer Photocopy Room is preheating to 230 DEG C, and shaping substrate is preheating to 180 DEG C, then by certainly
PEI silk material is sent into the printing head being preheated by dynamic wire feeder, and 3D printing spray head is according to the trajectory path of Software Create
It is successively printed, until printing is completed to obtain PEI bracket, then obtained PEI bracket is put into 150 DEG C of oven and toasts 15
Minute can be completed；
Step 4: being 10mmol/L in concentration, dopamine is added in the Tris-HCl buffer of PH=8.5, obtained concentration is
PEI bracket obtained in step 3 is immersed in the dopamine solution that concentration is 2mg/ml by the dopamine solution of 2mg/ml,
37 DEG C of constant temperature and it is protected from light processing 24 hours under conditions of coming into full contact with air, obtains the surface PEI bracket modified through dopamine,
Then modified PEI bracket is carried out after rinsing 3 times repeatedly with deionized water, then carries out freeze-drying preservation；
Step 5: being 10mmol/L in concentration, rgd peptide is added in the Tris-HCl buffer of PH=8.5, concentration is made
It is 200 μ g/ml's by concentration is soaked in through the amine-modified PEI bracket of DOPA in step 4 for the rgd peptide solution of 200 μ g/ml
In rgd peptide solution, it is placed in taking-up PEI bracket after handling 24 hours on 37 DEG C of constant-temperature tables, then repeatedly with deionized water
The rgd peptide for washing off the unbonded securely remnants of PEI rack surface, the freeze-drying of PEI bracket is saved backup, be made after having handled
It can promote the PEI bracket of bone tissue reparation.
In order to protrude technical effect of the invention, following comparative example 1-2 is provided with for embodiment 3 and is compared:
Comparative example 1: the PEI bracket obtained after 3D printing after having impregnated dopamine solution, no longer impregnates rgd peptide
Solution, other preparation process are identical as the preparation process in embodiment 3.
Comparative example 2: the PEI bracket obtained after 3D printing is directly dipped into rgd peptide solution, does not impregnate DOPA
Amine aqueous solution, other preparation process are identical as the preparation process in embodiment 3.
12 volumes, size, rabbit similar in health status are chosen, is divided into 4 groups, is established in rabbit left fore same position
Critical size defects, and identical bone collection operation is carried out to rabbit, so the bone of transplanting is embodiment 3 and comparative example 1-
PEI bracket obtained in 2, the 2nd, 4,8,12 week bat X piece check that situation is repaired at rabbit bone defect position, and at 12 weeks taking-up radius portion
Divide and observed, specific experimental result is as shown in table 1:
Table 1
By the result in table 1 it is found that the PEI bracket that 3D printing obtains in the present invention is passing through dopamine solution, rgd peptide
The immersion of solution can further promote the adhesion of cell, promote the reparation of bone tissue, and in comparative example 1,2 only
By PEI bracket be dipped into dopamine solution, rgd peptide solution one of solution in, PEI bracket can not be made to have at all
Promote the effect of the reparation of bone tissue, thus in comparative example 1,2 repair time of rabbit than in embodiment 3 when the reparation of rabbit
Between it is long；PEI bracket is not toasted after printing PEI bracket in comparative example 1, is bonded not between each layer of PEI bracket
Enough securely after carrying out bone collection to rabbit, implantation material bonding is insecure, is more easily damaged, can recovery and walking to rabbit
It impacts.
Each embodiment in this specification is described in a progressive manner, the highlights of each of the examples are with other
The difference of embodiment, the same or similar parts in each embodiment may refer to each other.
The foregoing description of the disclosed embodiments enables those skilled in the art to implement or use the present invention.
Various modifications to these embodiments will be readily apparent to those skilled in the art, as defined herein
General Principle can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, of the invention
It is not intended to be limited to the embodiments shown herein, and is to fit to and the principles and novel features disclosed herein phase one
The widest scope of cause.
Claims (7)
1. a kind of method of the bone filling bracket of 3D printing PEI surface recombination RGD, which is characterized in that including following preparation step:
Step 1: choosing PEI particle, PEI particle is placed in 140 DEG C -180 DEG C of air circulating oven and is dried 3-5 hours, make it
Sufficiently drying can stop, the particle after drying being put into wire drawing machine cartridge, to drawing until when moisture is less than 0.02%w/w
Silk machine barrel is heated, until temperature reaches 340-420 DEG C of stopping, after the PEI particle in wire drawing machine cartridge melts completely,
It is squeezed, PEI is squeezed into filiform, the PEI silk squeezed again passes by 140 DEG C -180 DEG C of air circulating oven, dries
It is 3-5 hours dry, dry PEI silk is obtained, is then sealed preservation for PEI；
Step 2: according to the shape of required bone filling bracket, the bracket knot of pre-print modeling: is established using 3 d modeling software
Structure model, and being translated into 3D printing system can be with identified stl file；
Step 3: stl file obtained in the step 2 being imported into 3D printer system, by automatic temperature control system to 3D
Printer head, Photocopy Room, shaping substrate are preheated, and are then preheated PEI silk material feeding by automatic wire feeding device
Printing head in, 3D printing spray head is successively printed according to the trajectory path of Software Create, until printing complete obtain PEI
Bracket, then obtained PEI bracket is put into toast 15-30 minutes in 100-150 DEG C of oven and can be completed；
Step 4: PEI bracket obtained in the step 3 being immersed in the dopamine solution that concentration is 2mg/ml, in 37 DEG C of perseverances
Temperature and it is protected from light processing 12-24 hour under conditions of coming into full contact with air, obtains PEI bracket of the surface through dopamine modification, so
Modified PEI bracket is carried out after rinsing 2-3 times repeatedly with deionized water afterwards, then carries out freeze-drying preservation；
Step 5: the rgd peptide solution that concentration is 200 μ g/ml will be soaked in through the amine-modified PEI bracket of DOPA in the step 4
In, it is placed in taking-up PEI bracket after handling 12-24 hours on 37 DEG C of constant-temperature tables, is then removed with deionized water repeated flushing
The rgd peptide of the unbonded securely remnants of PEI rack surface, saves backup the freeze-drying of PEI bracket after having handled, being made can promote
The PEI bracket of bone tissue reparation.
2. a kind of method of the bone filling bracket of 3D printing PEI surface recombination RGD according to claim 1, feature
It is, waits 3-5 seconds after the temperature of wire drawing machine cartridge reaches 340-420 DEG C in the step 1, melt completely to PEI particle
Afterwards, then to PEI it squeezes.
3. according to claim 1 or a kind of method of the bone filling bracket of 3D printing PEI surface recombination RGD described in 2, special
Sign is that the internal diameter of wire drawing machine cartridge extrusion is 1.5-2.0mm in the step 1.
4. a kind of method of the bone filling bracket of 3D printing PEI surface recombination RGD according to claim 1, feature
It is, in the step 1 when being sealed preservation to PEI, 2-3 packet silica dehydrator is put into the device being sealed
Agent.
5. a kind of method of the bone filling bracket of 3D printing PEI surface recombination RGD according to claim 1, feature
It is, 3D printer spray head is preheating to 360-415 DEG C by automatic temperature control system in the step 3, and printer Photocopy Room is preheating to
210-230 DEG C, shaping substrate is preheating to 110-180 DEG C.
6. a kind of method of the bone filling bracket of 3D printing PEI surface recombination RGD according to claim 1, feature
It is, is 10mmol/L in concentration, dopamine is added in the Tris-HCl buffer of PH=8.5, concentration in the step 4 is made
For the dopamine solution of 2mg/ml.
7. a kind of method of the bone filling bracket of 3D printing PEI surface recombination RGD according to claim 1, feature
It is, is 10mmol/L in concentration, rgd peptide is added in the Tris-HCl buffer of PH=8.5, is made dense in the step 5
Degree is the rgd peptide solution of 200 μ g/ml.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
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| CN201910507236.7A CN110368523A (en) | 2019-06-12 | 2019-06-12 | A kind of method of the bone filling bracket of 3D printing PEI surface recombination RGD |
| ZA2020/01584A ZA202001584B (en) | 2019-06-12 | 2020-03-13 | Method for 3d printing bone filling scaffold with pei surface composited with rgd |
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| CN201910507236.7A CN110368523A (en) | 2019-06-12 | 2019-06-12 | A kind of method of the bone filling bracket of 3D printing PEI surface recombination RGD |
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