CN1568904A - Preparation method of chitosan tubular bracket - Google Patents
Preparation method of chitosan tubular bracket Download PDFInfo
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- CN1568904A CN1568904A CNA2004100090911A CN200410009091A CN1568904A CN 1568904 A CN1568904 A CN 1568904A CN A2004100090911 A CNA2004100090911 A CN A2004100090911A CN 200410009091 A CN200410009091 A CN 200410009091A CN 1568904 A CN1568904 A CN 1568904A
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- Materials For Medical Uses (AREA)
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Abstract
A preparation method of chitosan tubular stent, which uses chitosan yarn as raw material, preparing hollow net tube structures of different specifications by means of spinning, and then inserting corresponding core model, coating chitosan, collagen, or gelatin biomacromolecule liquor, withdrawing the core model after drying, and obtaining a chitosan tube with wall thickness of approximately 0.5~2.5mm and inside diameter of 1~20mm. The method is simple and easy, and is suitable for mass production. The chitosan tube prepared by the invention has the characteristics of thick wall, controllable inside diameter, optional length, modeling in one go, good elasticity and intensity, and firm saturation in operation. All the raw materials in the preparation process adopts natural biodegradable materials, with their metabolic products having no toxic and side effects on human body, and are suitable for being used as tissue repair materials or tissue engineering stent materials.
Description
Technical field
The present invention relates to a kind of preparation method of chitosan tubulose support, belong to bio-medical material and tissue engineering technique field.
Background technology
In regenerative medicine and field of tissue engineering technology, the damage of multiple organ, tissue can be used tube as the timbering material of repairing, as artificial neural canal (comprising that peripheral nerve injury is with repairing conduit and spinal cord injury with repairing conduit), artificial trachea, artificial blood vessel etc.Adopt Biodegradable material to prepare support and can avoid second operation, alleviate patient's misery.The biodegradation material of synthetic has copolymer (PLGA), polycaprolactone (PCL) of polylactic acid (PLA), polyglycolic acid (PGA), hydroxyacetic acid and lactic acid etc.Synthetic material has the advantage of easy machine-shaping, and still, synthesis technique is complicated, the cost height; And somebody worker's synthetic material metabolite is acidic materials, and body is had stimulation, limited it in Clinical Application.The timbering material of natural origin is another direction of studying at present.
Chitosan is a kind of polysaccharide of natural origin, low price, and good biocompatibility, biodegradable, the metabolite nonirritant, and have the wound healing of promotion and antimicrobial function, be widely used in promoting in wound healing and the drug delivery system.Simultaneously, chitosan is easy to be processed to different shapes such as hollow pipe, film, spongy, fiber etc., is widely used in recent years in the biomedical research field.The processing chitosan makes it to become tubular bracket and is applied to the concern that regenerative medicine and organizational project have obtained a lot of scholars.
But chitosan does not have fusing point, and intensification can only carbonization and can not be softened or fuse, so can not be processed into pipe by methods such as extrusion moldings.Chinese patent (ZL99123745.5) " chitosan catheter that is used for neural reparation " discloses and has adopted the rotary evaporation legal system to be equipped with the method for chitosan conduit, but this method wastes time and energy, the pipe atresia of preparation and inhomogeneous, and be difficult to the chitosan conduit of preparation length greater than 20mm.Chinese patent application (publication number: CN1404881A) " be used for the neural chitosan catheter of repairing " and disclose and used specific mould, the freeze drying process that employing has improved, prepare the porous chitosan pipe, aspect much, overcome the shortcoming of prior art.But main process is passed through manual operations, difficult quality control between criticizing and criticizing.Therefore, study a kind of simple for processly, be fit to large-scale production, can prepare that wall thickness, internal diameter are controllable, the method for the welcome chitosan conduit of length, have important practical application meaning.
In recent years, the timbering material of textile technology preparation is used for tissue repair or organizational project receives publicity because of its distinctive advantage; Simultaneously, the research and development of pure chitosan (or chitin) fiber or yarn also is widely used in practice.Yet at present research and utilization chitosan (or chitin) fiber or yarn mainly are preparation mixed textile material and Healthful needles fabric (Chinese patent application number 00125896.6,00125895.8,00125894.X, 02152599.4,02270471.X, 03111868.2,03141755.8,03141756.6,03232769.2); The various devices (Chinese patent application number 01107011.0,03150483.3,03150485.X) that absorb that have also that its preparation fibre reinforced materials of research and utilization is used for that bone surgery uses.But there is not direct applicable industry textile technology processing chitosan yarn to prepare the research report of tissue engineering tubular scaffold.
Summary of the invention
The invention provides a kind of method that adopts textile technology processing and preparing chitosan tubulose support.Utilize that this method can prepare that wall thickness, internal diameter are controllable, the porous arbitrarily chitosan conduit of length or have the outer surface of smooth densification and the chitosan conduit of the special construction of softish fibroid inner surface, and can be fit to large-scale production.
The present invention is achieved by following technical proposals:
A kind of preparation method of chitosan tubulose support is characterized in that this method carries out as follows:
1) be raw material with 100% chitosan yarn, adopt existing industrialization weaving conventional method, it is that 1～20mm, wall thickness are the chitosan hollow webmaster of 0.5～2.5mm that the chitosan yarn is made internal diameter;
2) in the core insertion tube of selection and hollow webmaster respective length and diameter, be impregnated in the biogenetic derivation degradable macromolecule solution, take out, make webmaster surface formation coat in the chitosan hollow webmaster surface-coated of core support or with it; Or before inserting core, earlier chitosan hollow webmaster is impregnated in the biogenetic derivation degradable macromolecule solution, insert core then, be impregnated into again in the macromolecular solution, take out; Described biogenetic derivation degradable macromolecule solution is meant the mixed solution of chitosan solution, collagen solution or gelatin solution or above solution; Described chitosan solution is 1～3% (w/v) weak acid solution, and described collagen solution is the hydrochloric acid solution of 0.5～3% (w/v), and described gelatin solution is the aqueous solution of 10～20% (w/v);
3) with step 2) in the chitosan conduit that scribbles coat that makes place 40 ℃～70 ℃ baking ovens to toast 0.5～3 hour, take out then and dry or lyophilization;
4) with exsiccant chitosan conduit, place alkali liquor to soak 10～30 minutes, take out,, be dipped in again in the phosphate buffer of pH=7.2～7.4 10～30 minutes to neutral with rinsed with deionized water, draw surface moisture with filter paper, natural drying; Wherein said alkali liquor is sodium hydroxide, potassium hydroxide or aqueous sodium carbonate or its mixed solution of 2～20% (w/v);
5) take out core, make chitosan conduit.
The present invention directly utilizes the chitosan yarn to prepare tissue injury and repairs and the used in tissue engineering tubular bracket, overcome and prepared the technologic a lot of shortcomings of chitosan conduit in the past, the advantage that has kept textile technology gained support, can prepare wall thickness, internal diameter is controllable, the porous arbitrarily chitosan conduit of length or have the outer surface of smooth densification and the chitosan tubulose support of the special construction of softish fibroid inner surface, not only creep to cell in space between gained support center line and line, condition has been created in breeding, has created good environment also for the ectendotrophy exchange of tissue and organ; But support size and length approbate and reprobate, one-shot forming; Mechanical property is good, has good elasticity and intensity; Stitching is connected firmly in the operation, and simple for process, is fit to large-scale production.
The specific embodiment
With commercially available 100% chitosan yarn is raw material, adopts existing industrialization weaving conventional method, and according to different application purposes, it is 1～20mm, length hollow webmaster arbitrarily that the chitosan yarn is weaved into internal diameter.Adjust the number of different number yarn plying, make pipe thickness be about 0.5～2.5mm.Select the chitosan hollow webmaster of certain-length, internal diameter and pipe thickness for use, in the core insertion tube with respective length and diameter, make chitosan hollow webmaster keep certain shape.Core is to be that the club or the inner core of circle is stainless steel bar with the cross section that rustless steel or polytetrafluoroethylmaterial material are made, and skin is the circular club of polyfluortetraethylene pipe, and its diameter is identical with the internal diameter of respective shell polysaccharide hollow webmaster.At chitosan hollow webmaster surface-coated chitosan, collagen or the gelatin solution that core supports, perhaps be immersed in this solution and take out then, drying makes netted tube-surface form the coat of chitosan, collagen or gelatin.When forming the coat of chitosan, collagen or gelatin etc., the chitosan solution of preferred 1～3% (w/v), used deacetylating degree of chitosan 80～95% is the weak acid preparation of 1～15% (v/v) with concentration, weak acid is acetic acid or phosphoric acid, citric acid, lactic acid; Or the collagen solution of 0.5～3% (w/v), used collagen can be the mixture of type i collagen or type i collagen and III Collagen Type VI, with the hydrochloric acid preparation of 0.5～2mol/L; Or the aqueous gelatin solution of 10～20% (w/v); Or the mixed solution of above solution.
When comprising that for making netted chitosan conduit all mesh all form coat from the inwall to the outer wall, can before inserting core, earlier chitosan hollow webmaster be immersed in chitosan, collagen or the gelatin solution, insert core then, be impregnated into again in chitosan, collagen or the gelatin solution, take out dry.
For improving the intensity of hollow webmaster, repeatedly repeated impregnations and drying steps make the thicker conduit of coat.
For improving chitosan conduit anti-degradation capability in vivo, prolong artificial neural canal and keep the piped time in vivo, can carry out crosslinking Treatment.Crosslinking Treatment can be before coat forms, also can be after coat forms.Cross-linking method can select that conventional gamma-rays is crosslinked for use, ultraviolet-crosslinkable, hot dehydrated crosslinking, glutaraldehyde cross-linking, epoxide is crosslinked and water-soluble carbodiimide is crosslinked.For controlling crosslinking degree easily, do not introduce chemical substance in the cross-linking process, preferably adopt hot dehydrated crosslinking.Hot dehydrated crosslinking is handled and carry out 110～150 ℃ of temperature, the about 12～48h of crosslinking time under vacuum, hot conditions.
For obtaining porous tube, can be earlier with the chitosan hollow webmaster of surface-coated chitosan, collagen or gelatin solution place-20 ℃～-196 ℃ freezing 12～48 hours.Then freezing chitosan conduit complete, that freeze molding is placed freezer dryer lyophilization (-40～-80 ℃ approximately of trunk temperature, 12～48 hours drying times), remove moisture.
For obtaining the tube of outer wall dense non-porous, can be earlier place 40 ℃～70 ℃ baking ovens to toast 0.5～3 hour the chitosan hollow webmaster of surface-coated chitosan, collagen or gelatin solution, take out then and put room temperature and dry naturally.
With exsiccant chitosan conduit, place alkali liquor to soak 10～30 minutes, remove residual acetic acid.Alkali liquor is that concentration is sodium hydroxide, potassium hydroxide or sodium carbonate liquor or its mixed solution of 2～20% (w/v), also can contain the ethanol of 5～30% (v/v) simultaneously.From alkali liquor, take out chitosan conduit, to neutral, be dipped in again in the phosphate buffer of pH=7.2～7.4 10～30 minutes with rinsed with deionized water.Draw chitosan conduit surface moisture, natural drying with filter paper.Take out core, make chitosan conduit.
Can further specify the present invention by the following examples.
Embodiment 1:
1) with 2 plying of chitosan yarn of 21, adopt the suitable tension force and the speed of travel of method adjustment of warp-wise braiding, being woven into internal diameter is 3mm, the hollow webmaster of the about 1.0mm of wall thickness;
2) getting this hollow webmaster 300mm, is that insert in the hollow webmaster for circular rustless steel core in the cross section of 3mm with diameter;
3) chitosan 1 gram of getting deacetylation 83% is dissolved among the 1% acetic acid solution 100ml, and stirring and dissolving is mixed with the chitosan solution of 1% (w/v).After treating that chitosan dissolves fully, 200 order nylon cloths filter, and the room temperature decompression is bled, and eliminates the bubble in the solution;
4) chitosan conduit that will have core to support is immersed in the chitosan solution of removing bubble, slowly rotation, the vertical taking-up.
5) chitosan conduit that will scribble the chitosan coat places 40 ℃ of baking ovens bakings 3 hours, takes out from baking oven and puts room temperature, dries.
6) exsiccant chitosan conduit is placed 2% sodium hydroxide solution soaked 10 minutes, remove remaining acid.Deionized water wash is to neutral, puts in the PBS buffer of pH=7.4 30 minutes again.Draw chitosan conduit surface moisture, natural drying with filter paper.
(7) take out core, make the chitosan pipe.
The effect of present embodiment: making length is 300mm, and internal diameter 3mm, wall thickness are about 1.1mm, has the outer surface of smooth densification and the chitosan conduit of softish fibroid inner surface.Outer surface chitosan layer applies evenly, and is sparkling and crystal-clear transparent, and inner weaving tube clean mark as seen.
Embodiment 2:
1) with 2 plying of chitosan yarn of 21, adopt the suitable tension force and the speed of travel of method adjustment of warp-wise braiding, being woven into internal diameter is 6mm, the hollow webmaster of the about 1.0mm of wall thickness;
2) getting this hollow webmaster 100mm, is that insert in the hollow webmaster for circular politef core in the cross section of 6mm with diameter.
3) chitosan 3 gram of getting deacetylation 90% is dissolved among the 5% acetic acid solution 100ml, and stirring and dissolving is mixed with the chitosan solution of 3% (w/v).After treating that chitosan dissolves fully, 200 order nylon cloths filter.Prepare the aqueous gelatin solution 100ml of 20% (w/v) again, the two mixing, 50 ℃ of heated and stirred are to even, and the room temperature decompression is bled, and eliminates the bubble in the solution.
4) chitosan conduit that will have core to support is immersed in the chitosan of removing bubble, the gelatin cross-blend solution, slowly rotation, the vertical taking-up.
5) chitosan conduit that will scribble chitosan and gelatin coat places 60 ℃ of baking ovens bakings 1 hour, takes out from baking oven and puts room temperature, dries.
6) exsiccant chitosan conduit is placed 20% sodium hydroxide solution soaked 10 minutes, remove remaining acid.Deionized water wash is to neutral, puts in the PBS buffer of pH=7.4 30 minutes again.Draw chitosan conduit surface moisture, natural drying with filter paper.
(7) take out core, make the chitosan pipe.
The effect of present embodiment: making length is 100mm, and internal diameter 6mm, wall thickness are about 1.1mm, has the chitosan tubulose support of chitosan and gelatin coating.
Embodiment 3:
1) with 4 plying of chitosan yarn of 21, adopt the suitable tension force and the speed of travel of method adjustment of warp-wise braiding, being woven into internal diameter is 1mm, the hollow webmaster of the about 0.5mm of wall thickness;
2) get this hollow webmaster 200mm, the cross section that with diameter is 1mm is for circular, and inner core is that stainless steel bar, outer bar-shaped core for polyfluortetraethylene pipe insert in the hollow webmaster;
3) chitosan 2 gram of getting deacetylation 93% is dissolved among the 1% acetic acid solution 100ml, and stirring and dissolving is mixed with the chitosan solution of 2% (w/v).After treating that chitosan dissolves fully, 200 order nylon cloths filter, and the room temperature decompression is bled, and eliminates the bubble in the solution.
4) chitosan conduit that will have core to support is immersed in the chitosan solution of removing bubble, slowly rotation, the vertical taking-up.
5) chitosan conduit that will scribble the chitosan coat place-20 ℃ freezing 48 hours.Then freezing chitosan conduit complete, that freeze molding is placed freezer dryer lyophilization (-80 ℃ approximately of trunk temperature, 24 hours drying times), remove moisture.
6) exsiccant chitosan conduit is placed 2% sodium hydroxide solution soaked 10 minutes, remove remaining acid.Deionized water wash is to neutral, puts in the PBS buffer of pH=7.4 30 minutes again.Draw chitosan conduit surface moisture, natural drying with filter paper.
7) take out core, make the porous chitosan tubular bracket.
The effect of present embodiment: making length is 200mm, and internal diameter 1mm, wall thickness are about 0.7mm, porous chitosan tubulose support.
Embodiment 4:
1) with 4 plying of chitosan yarn of 40, adopt the suitable tension force and the speed of travel of method adjustment of warp-wise braiding, being woven into internal diameter is 5mm, the hollow webmaster of the about 1.0mm of wall thickness;
2) getting this hollow webmaster 150mm, is that insert in the hollow webmaster for circular politef core in the cross section of 5mm with diameter;
3) with the type i collagen solution of the hydrochloric acid of 1mol/L preparation 3% (w/v);
4) chitosan conduit that core supports be will have and slowly rotation, the vertical taking-up will be immersed in the collagen solution;
5) chitosan conduit that will scribble the collagen coat place-20 ℃ freezing 24 hours.Then freezing chitosan conduit complete, that freeze molding is placed freezer dryer lyophilization (-40 ℃ approximately of trunk temperature, 48 hours drying times), remove moisture;
6) exsiccant chitosan conduit is placed 10% potassium hydroxide solution soaked 10 minutes, remove remaining acid.Deionized water wash is to neutral, puts in the PBS buffer of pH=7.4 30 minutes again.Draw chitosan conduit surface moisture, natural drying with filter paper.
7) take out core, make the chitosan pipe.
The effect of present embodiment: making length is 150mm, and internal diameter 5mm, wall thickness are about 1.2mm, and the surface has the porous chitosan tubular bracket of collagenic coating.
Embodiment 5:
1) with 6 plying of chitosan yarn of 40, adopt the suitable tension force and the speed of travel of method adjustment of warp-wise braiding, being woven into internal diameter is 3mm, the hollow webmaster of the about 1.2mm of wall thickness;
2) getting this hollow webmaster 100mm, is that insert in the hollow webmaster for circular rustless steel core in the cross section of 3mm with diameter.
3) chitosan conduit that will have core to support carries out hot dehydrated crosslinking processing, and hot dehydrated crosslinking is handled and carry out 120 ℃ of temperature, the about 24h of crosslinking time under vacuum, hot conditions;
4) chitosan 1 gram of getting deacetylation 90% is dissolved among the 5% citric acid solution 100ml, and stirring and dissolving is mixed with the chitosan solution of 1% (w/v).After treating that chitosan dissolves fully, 200 order nylon cloths filter, and the room temperature decompression is bled, and eliminates the bubble in the solution;
5) chitosan conduit of handling through overheated dehydrated crosslinking that core supports be will have and slowly rotation, the vertical taking-up will be immersed in the chitosan solution of removing bubble;
6) chitosan conduit that will scribble the chitosan coat places 40 ℃ of baking ovens bakings 3 hours, takes out from baking oven and puts room temperature, dries;
7) exsiccant chitosan conduit is placed contain 30% alcoholic acid 2% sodium hydroxide solution and soaked 10 minutes, remove remaining acid.Deionized water wash is to neutral, puts in the PBS buffer of pH=7.4 20 minutes again.Draw chitosan conduit surface moisture, natural drying with filter paper.
8) take out core, make the chitosan pipe.
The effect of present embodiment: making length is 100mm, and internal diameter 3mm, wall thickness are about 1.2mm, fine and close chitosan tubulose support.
Embodiment 6:
1) with 6 plying of chitosan yarn of 21, adopt the suitable tension force and the speed of travel of method adjustment of warp-wise braiding, being woven into internal diameter is 4mm, the hollow webmaster of the about 1.4mm of wall thickness;
2) chitosan 1 gram of getting deacetylation 85% is dissolved among the 1% acetic acid solution 100ml, and stirring and dissolving is mixed with the chitosan solution of 1% (w/v).After treating that chitosan dissolves fully, 200 order nylon cloths filter, and the room temperature decompression is bled, and eliminates the bubble in the solution;
3) get this hollow webmaster 180mm, it slowly is immersed in the chitosan solution, take out immediately, the cross section that with diameter is 4mm is for circular, and inner core is that stainless steel bar, outer bar-shaped core for polyfluortetraethylene pipe insert in the hollow webmaster;
4) chitosan conduit that core supports that has that will be soaked with chitosan solution is immersed in the chitosan solution slowly rotation, the vertical taking-up once more;
5) chitosan conduit that inside and outside wall is all scribbled the chitosan coat places 70 ℃ of baking ovens bakings 0.5 hour, takes out from baking oven and puts room temperature, dries;
6) exsiccant chitosan conduit is placed contain 20% alcoholic acid 5% sodium carbonate liquor and soaked 30 minutes, remove remaining acid.Deionized water wash is to neutral, puts in the PBS buffer of pH=7.4 30 minutes again.Draw chitosan conduit surface moisture, natural drying with filter paper;
7) take out core, make the chitosan pipe.
The effect of present embodiment: making length is 180mm, and internal diameter 4mm, wall thickness are about 1.4mm, fine and close chitosan tubulose support.
Embodiment 7:
1) with 8 plying of chitosan yarn of 21, adopting knitting method to make internal diameter is 20mm, the hollow webmaster of the about 2.5mm of wall thickness;
2) getting this hollow webmaster 200mm, is that insert in the hollow webmaster for circular rustless steel core in the cross section of 20mm with diameter;
3) chitosan 3 gram of getting deacetylation 85% is dissolved among the 2% acetic acid solution 100ml, and stirring and dissolving is mixed with the chitosan solution of 3% (w/v).After treating that chitosan dissolves fully, 200 order nylon cloths filter, and the room temperature decompression is bled, and eliminates the bubble in the solution;
4) chitosan conduit that will have core to support is immersed in the chitosan solution of removing bubble, slowly rotation, the vertical taking-up;
5) chitosan conduit that will scribble the chitosan coat places the chitosan conduit that will have core to support to carry out hot dehydrated crosslinking to handle.Hot dehydrated crosslinking is handled and carry out 140 ℃ of temperature, the about 12h of crosslinking time under vacuum, hot conditions;
6) chitosan conduit of heat cross-linking after drying is placed contain 5% sodium carbonate liquor and soaked 20 minutes, remove remaining acid.Deionized water wash is to neutral, puts in the PBS buffer of pH=7.4 30 minutes again.Draw chitosan conduit surface moisture, natural drying with filter paper;
7) take out core, make the chitosan pipe.
The effect of present embodiment: making length is 200mm, and internal diameter 20mm, wall thickness are about 2.5mm, fine and close chitosan tubulose support.
Embodiment 8:
1) with 8 plying of chitosan yarn of 21, adopting knitting method to make internal diameter is 5mm, the hollow webmaster of the about 1.0mm of wall thickness;
2) get this hollow webmaster 200mm, take out the back in the type i collagen of immersion 3% (w/v) and the mixed solution of III Collagen Type VI, is that the cross section of 5mm is a stainless steel bar for circular, inner core with diameter, and skin is in the core insertion hollow webmaster of polyfluortetraethylene pipe.Immerse again in the epoxy glue original solution, take out;
3) chitosan conduit that will scribble the collagen coat places 70 ℃ of baking ovens bakings 0.5 hour, takes out from baking oven and puts room temperature, dries.
4) it is immersed back taking-up in the epoxy glue original solution, oven dry so repeats 10 times again;
5) exsiccant chitosan conduit is placed 10% sodium hydroxide solution soaked 10 minutes, remove remaining acid.Deionized water wash is to neutral, puts in the PBS buffer of pH=7.4 30 minutes again.Draw chitosan conduit surface moisture, natural drying with filter paper;
6) take out core, the chitosan pipe of collagenic coating is all arranged inside and outside making.
The effect of present embodiment: making length is 200mm, internal diameter 5mm, and the about 1.3mm of wall thickness is coated with the chitosan conduit of collagen layer inside and outside the pipe.
Claims (7)
1. the preparation method of a chitosan tubulose support is characterized in that this method carries out as follows:
1) be raw material with 100% chitosan yarn, adopt existing industrialization weaving conventional method, it is that 1～20mm, wall thickness are the chitosan hollow webmaster of 0.5～2.5mm that the chitosan yarn is made internal diameter;
2) in the core insertion tube of selection and hollow webmaster respective length and diameter, be impregnated in the biogenetic derivation degradable macromolecule solution, take out, make webmaster surface formation coat in the chitosan hollow webmaster surface-coated of core support or with it; Or before inserting core, earlier chitosan hollow webmaster is impregnated in the biogenetic derivation degradable macromolecule solution, insert core then, be impregnated into again in the macromolecular solution, take out; Described biogenetic derivation degradable macromolecule solution is meant the mixed solution of chitosan solution, collagen solution or gelatin solution or above solution; Described chitosan solution is 1～3% (w/v) weak acid solution, and described collagen solution is the hydrochloric acid solution of 0.5～3% (w/v), and described gelatin solution is the aqueous solution of 10～20% (w/v);
3) with step 2) in the chitosan conduit that scribbles coat that makes place 40 ℃～70 ℃ baking ovens to toast 0.5～3 hour, take out then and dry or its lyophilization;
4) with exsiccant chitosan conduit, place alkali liquor to soak 10～30 minutes, take out,, be dipped in again in the phosphate buffer of pH=7.2～7.4 10～30 minutes to neutral with rinsed with deionized water, draw surface moisture with filter paper, natural drying; Wherein said alkali liquor is that concentration is sodium hydroxide, potassium hydroxide or sodium carbonate liquor or its mixed solution of 2～20% (w/v);
5) take out core, make chitosan conduit.
2. according to the described preparation method of claim 1, it is characterized in that: described core is that circular club or the inner core made with rustless steel or polytetrafluoroethylmaterial material are stainless steel bar, and skin is the circular club of polyfluortetraethylene pipe.
3. according to the described preparation method of claim 1, it is characterized in that: described chitosan solution adopts the chitosan of deacetylation 80～95%, is the weak acid preparation of 1～15% (v/v) with concentration, and described weak acid is acetic acid, phosphoric acid, citric acid or lactic acid.
4. according to the described preparation method of claim 1, it is characterized in that: described collagen is the mixture of type i collagen or type i collagen and III Collagen Type VI, is the hydrochloric acid solution preparation of 0.5～2mol/L with concentration.
5. according to the described preparation method of claim 1, it is characterized in that: in described step 2) in, before coat forms or after coat forms, the chitosan webmaster is carried out crosslinking Treatment.
6. according to the described preparation method of claim 5, it is characterized in that: described crosslinking Treatment adopts 110～150 ℃ of temperature, and crosslinking time is the hot dehydrated crosslinking of 12～48h.
7. according to the described preparation method of claim 1, it is characterized in that: the ethanol that contains 5～30% (v/v) in the alkali liquor in described step 4).
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