CN101502671B - Method for preparing silk fibroin/ P(LLA-CL) compound nano fiber structure repair stand - Google Patents
Method for preparing silk fibroin/ P(LLA-CL) compound nano fiber structure repair stand Download PDFInfo
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- CN101502671B CN101502671B CN 200910045769 CN200910045769A CN101502671B CN 101502671 B CN101502671 B CN 101502671B CN 200910045769 CN200910045769 CN 200910045769 CN 200910045769 A CN200910045769 A CN 200910045769A CN 101502671 B CN101502671 B CN 101502671B
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Abstract
The invention relates to a method for preparing fibroin protein/P(LLA-CL) compound nanofiber tissue repair bracket, comprising : (1) boiling silkworm cocoon with pupa removed in 0.5% of Na2CO3 aqueous solution, then cleaning and drying the boiled silkworm cocoon; then dissolving the boiled silkworm cocoon in ternary solvent, carrying out hydrolysis at constant temperature, dialyzing for 70-72 hours, carrying out prefreezing, then carrying out freezing and drying at the temperature of minus (45-58) DEG C; (2) dissolving the fibroin protein in hexafluoroisopropanol, stirring to dissolve the protein; (3) dissolving the P(LLA-CL) in hexafluoroisopropanol, stirring to dissolve the fiber; (4) mixing the fibroin protein solution with P(LLA-CL) solution, stirring the solution evenly; (5) carrying out electrostatic spinning on blending electrostatic spinning liquid and obtaining the fibroin protein/P(LLA-CL) compound nanofiber. The preparation method of the invention is easy and feasible, the raw materials are abundant and the industrialized production is easy to be realized; in addition, the obtained compound nanofiber features fine biocompatibility, good mechanical property and relatively high porosity factor.
Description
Technical field
The invention belongs to the preparation field of electrostatic spinning composite nano fiber, particularly relate to the method for preparing of fibroin albumen/P (LLA-CL) complex nanometer fibrous tissue renovation bracket.
Background technology
The human sick tissue that decreases is rebuild or repaired to the developing into of organizational project and regenerative medicine provides the efficacious therapy means with organ.Biodegradable stent is that cell growth and tissue regeneration provide necessary space and support, for the signal conduction provides effective channel, is one of decision organizational project key of success factor.
The design of timbering material should bionical to greatest extent human body cell epimatrix (ECM) structure and functional characteristics, could effectively solve the biocompatibility issues of material, the conduction of regulation and control bio signal, inducing cell and tissue growth promote body regeneration and repair.Human inner cell's epimatrix is that fibre diameter is generally 50-500nm [1] by protein and the crosslinked nanofiber gel network that forms of ammonia polyose of candy (GAGs) in essence.Cell combine with ligand specificity on the extracellular matrix through the receptor system on the cell membrane and to external world signal make a response, influence cell behavior.
Fibroin albumen is by degumming silkworm cocoons, is a kind of natural protein, has excellent performance such as excellent biological compatibility, biodegradability, high ventilation, poisture-penetrability, non-immunogenicity.Be widely used in the reparation and the regeneration of tissues such as skin, bone, cartilage, tendon, nerve trachea, blood vessel.The silk fibroin nano-fiber film that Park etc. [2] are equipped with for the solvent electro-spinning with formic acid can promote mouse preosteoblast (MC3T3-E1) to adhere to, breed, break up; Can induce the generation of Bone Gla protein; Promote bone tissue restoration and regeneration; Chinese patent CN1844509A discloses " a kind of method for preparing of silk fibroin porous material "; Be with water for the solvent electro-spinning has been equipped with good biocompatibility, nanofiber that hole is high, but single silk fibroin nano-fiber matter is crisp, pliability is poor, has reduced in fact with being worth.The design of the best of tissue engineering bracket is from structure, form and function on bionic extracellular matrix, and offer tissue growth certain mechanics support be provided.
P (LLA-CL) is the copolymer of polylactic acid and polycaprolactone, because it has good biodegradability and favorable mechanical performance, in recent years, is widely used in organizational project and drug release field [3,4].But the disadvantage of P (LLA-CL) is can not be for tissue growth provides bio signal [5], is unfavorable for sticking and growing of cell.Therefore; Fibroin albumen and P (LLA-CL) blend can be combined the two advantage, can bio signal be provided, promote adhesion, propagation, the differentiation of cell for tissue growth; Have higher mechanical property and controlled degradation rate again, satisfy the needs that substitute function of organization.
Therefore; The composite nano fiber scaffold of fibroin albumen and P (LLA-CL) blend preparation is expected to filter out the ideal tissue engineering bracket of bionic extracellular matrix from the 26S Proteasome Structure and Function; Be specially adapted to the high tubular bracket of mechanical property requirements, like blood vessel, nerve trachea etc.And the method for the composite nano fiber of fibroin albumen and P (LLA-CL) blend preparation is not appeared in the newspapers so far.
List of references:
[1]Piez?KA.Molecular?and?aggregate?structures?of?the?collagens.In：Reddi?AH，editor.Extracellular?Matrix?Biochemistry.New?York：Elsevier，1984.1-35.
[2]Kim?KH，Jeong?L，Park?WH，et?al.Biological?efficacy?of?silk?fibroin?nanofiber?membranesfor?guided?bone?regeneration，J?Biotechnol，2005，120：327-339.
[3]Kwon?IK，Kidoaki?S，Matsuda?T.Electrospun?nano-to?microfiber?fabrics?made?ofbiodegradable?copolyesters：structural?characteristics，mechanical?properties?and?cell?adhesionpotential，Biomaterials，2005，26：3929-3939.
[4]Mo?XM，Xu?CY，Kotaki?M，Ramakrishna?S.Electrospun?P(LLA-CL)nanofiber：abiomimetic?extracellular?matrix?for?smooth?muscle?cell?and?endothelial?cell?proliferation，Biomaterials，2004，25：1883-1890.
[5]Kim?BS，Mooney?DJ.Development?of?biocompatible?synthetic?extracellular?matrices?fortissue?engineering.Trends?Biotechnol.1998，16：224-228
Summary of the invention
Technical problem to be solved by this invention provides the method for preparing of a kind of fibroin albumen/P (LLA-CL) complex nanometer fibrous tissue renovation bracket, and this method for preparing is simple, and raw material resources is abundant, is prone to realize suitability for industrialized production; Fibroin albumen/the P that makes (LLA-CL) composite nano fiber has excellent biological compatibility, favorable mechanical performance and higher porosity; And can be through the blend of different proportion; Obtain the composite nano fiber of different performance; Satisfy the needs of different tissue engineering brackets, as tissue recovery supports such as skin, cartilage, blood vessel, nerve tracheas.
The method for preparing of a kind of fibroin albumen/P of the present invention (LLA-CL) complex nanometer fibrous tissue renovation bracket comprises:
(1) will remove pupa Bombyx bombycis is 0.5%Na 100 ℃ mass percent
2CO
3Boil 3-5 time in the aqueous solution, each 30min-50min, fully clean with distilled water, in 45 ℃ of-48 ℃ of oven dry, it is 1: 2: 8 CaCl that the silk fiber after the oven dry is dissolved in mol ratio with bath raio 1: 10-15
2, C
2H
5OH and H
2The ternary solvent of O in 60 ℃ of-70 ℃ of constant temperature hydrolysis 1-2h, is used distill water dialysis 70-72h then, puts into again-(70-80) ℃ precooling 12-18h, and then in-(45-58) ℃ lyophilization, the cellular solid filament fibroin that obtains loosening;
(2) step (1) fibroin albumen is dissolved in hexafluoroisopropanol, is stirred to dissolving fully under the room temperature, obtain the silk fibroin protein solution that concentration is 4-10%g/ml;
(3) P (LLA-CL) is dissolved in hexafluoroisopropanol, is stirred to dissolving fully under the room temperature, obtain the P that concentration is 4-10%g/ml (LLA-CL) solution;
(4) silk fibroin protein solution and step (3) P (LLA-CL) solution are mixed; Wherein fibroin albumen accounts for the 10%-90% of solute gross mass, and P (LLA-CL) accounts for the 90%-10% of solute gross mass, and the mass and size percentage ratio that total soluble matters accounts for solvent is 4-10%; Stir, obtain blend electrostatic spinning liquid;
(5) step (4) blend electrostatic spinning liquid is carried out electrostatic spinning; Adjustment electrostatic spinning process parameter, control voltage is the 8-15 kilovolt, electric field distance is the 60-200 millimeter; Spinning speed be the 0.5-1.5 milliliter/hour, obtain fibroin albumen/P (LLA-CL) composite nano fiber.
P (LLA-CL) in the said step (3) is that mol ratio is 50: 50 the polylactic acid and the random copolymer of polycaprolactone.
Said step (4) solute is fibroin albumen and P (LLA-CL).
The diameter of said step (5) fibroin albumen/P (LLA-CL) composite nano fiber increases gradually with spinning solution concentration and increases, and reduces gradually with the increase of fibroin albumen content.
Described fibroin albumen/P (LLA-CL) composite nano fiber is applied to the research of biomedical sector organizational project.
Beneficial effect
(1) this method for preparing is simple, and raw material resources is abundant, is prone to realize suitability for industrialized production;
(2) fibroin albumen/P (LLA-CL) composite nano fiber of making of the present invention has excellent biological compatibility, favorable mechanical performance and higher porosity; And can be through the blend of different proportion; Obtain the composite nano fiber of different performance; Satisfy the needs of different tissue engineering brackets, as tissue recovery supports such as skin, cartilage, blood vessel, nerve tracheas;
(3) composite fibre that makes can be good at structure, composition and the function of bionical n cell epimatrix, is ideal tissue engineering bracket.
Description of drawings
Fig. 1 gross mass volume ratio is 8%, and wherein fibroin albumen/P (LLA-CL) is the stereoscan photograph of 50: 50 Combined Electrostatic spinning nano fibre
Fig. 2 gross mass volume ratio is 8%, and wherein fibroin albumen/P (LLA-CL) is the stereoscan photograph of 25: 75 Combined Electrostatic spinning nano fibre
Fig. 3 gross mass volume ratio is 8%, and wherein fibroin albumen/P (LLA-CL) is the stereoscan photograph of 50: 50 Combined Electrostatic spinning nanometer fiber membrane
Fig. 4 gross mass volume ratio is 8%, and wherein fibroin albumen/P (LLA-CL) is the stereoscan photograph of 50: 50 Combined Electrostatic spinning nano fibre pipe
The specific embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in the restriction scope of the present invention.Should be understood that in addition those skilled in the art can do various changes or modification to the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Earlier 100 gram Bombyx bombycis are joined 1 liter of 0.5%Na
2CO
3In the aqueous solution, boil 30min, reprocessing three times, fully clean with distilled water, the drying baker of putting into 45 ℃ after cleaning is dried the silk fiber after obtaining coming unstuck.With CaCl
2: C
2H
5OH: H
2O=1: 2: 8 mol ratio preparation ternary solvent, with the bath raio of silk fiber, place 70 ℃ the interior constant temperature hydrolysis 1h of water-bath with 1: 10, obtain the xanchromatic silk fiber hydrating solution of consoluet palm fibre.Hydrolyzed solution is packed in the bag filter, uses distill water dialysis 72h, and the silk fiber hydrating solution that dialysis is good is put into-80 ℃ of pre-freeze 12h, then-58 ℃ of lyophilizations to doing, obtain white, tasteless, loose cellular solid filament fibroin.
0.8 gram fibroin albumen is dissolved in 10 milliliters of hexafluoroisopropanols, is stirred to dissolving fully, obtain the silk fibroin protein solution that concentration is 8% (grams per milliliter); 0.8 gram P (LLA-CL) is dissolved in 10 milliliters of hexafluoroisopropanols, is stirred to dissolving fully, obtain the P that concentration is 8% (grams per milliliter) (LLA-CL) solution; With volume ratio 25: 75, the mass ratio of solute mixed at 25: 75 with two kinds of solution, obtained total concentration and be 8% mixed solution.Mixed solution is carried out electrostatic spinning, spinning condition: voltage, 12 kilovolts; Electric field distance, 130 millimeters; Spinning speed, obtains the composite nano-fiber membrane that fiber diameter is 237 nanometers by 1.2 milliliters/hour.
Embodiment two
Take by weighing fibroin albumen 0.8 gram, be dissolved in 10 milliliters of hexafluoroisopropanols, be stirred to dissolving fully, obtain the silk fibroin protein solution that concentration is 8% (grams per milliliter) according to preparation among the embodiment one; 0.8 gram P (LLA-CL) is dissolved in 10 milliliters of hexafluoroisopropanols, is stirred to dissolving fully, obtain the P that concentration is 8% (grams per milliliter) (LLA-CL) solution; With volume ratio 50: 50, the mass ratio of solute mixed at 50: 50 with two kinds of solution, obtained total concentration and be 8% mixed solution.Mixed solution is carried out electrostatic spinning, spinning condition: voltage, 12 kilovolts; Electric field distance, 130 millimeters; Spinning speed, obtains the composite nano-fiber membrane that fiber diameter is 212 nanometers by 1.2 milliliters/hour.
Embodiment three
Take by weighing fibroin albumen 0.8 gram, be dissolved in 10 milliliters of hexafluoroisopropanols, be stirred to dissolving fully, obtain the silk fibroin protein solution that concentration is 8% (grams per milliliter) according to preparation among the embodiment one; 0.8 gram P (LLA-CL) is dissolved in 10 milliliters of hexafluoroisopropanols, is stirred to dissolving fully, obtain the P that concentration is 8% (grams per milliliter) (LLA-CL) solution; With volume ratio 50: 50, the mass ratio of solute mixed at 50: 50 with two kinds of solution, obtained total concentration and be 8% mixed solution.Mixed solution is carried out electrostatic spinning, spinning condition: voltage, 12 kilovolts; Electric field distance, 130 millimeters; Spinning speed, 1.2 milliliters/hour, with roller bearing 20cm place under syringe needle of ground connection, take off behind the certain hour in through 3mm, wall thickness 0.2mm, fibroin albumen/P of length 5.5cm (LLA-CL) composite Nano tubulose support.
Embodiment four
Take by weighing fibroin albumen 1.0 grams, be dissolved in 10 milliliters of hexafluoroisopropanols, be stirred to dissolving fully, obtain the silk fibroin protein solution that concentration is 10% (grams per milliliter) according to preparation among the embodiment one; 1.0 gram P (LLA-CL) are dissolved in 10 milliliters of hexafluoroisopropanols, be stirred to dissolving fully, obtain the P that concentration is 10% (grams per milliliter) (LLA-CL) solution; With volume ratio 50: 50, the mass ratio of solute mixed at 50: 50 with two kinds of solution, obtained total concentration and be 10% mixed solution.Mixed solution is carried out electrostatic spinning, spinning condition: voltage, 12 kilovolts; Electric field distance, 130 millimeters; Spinning speed, obtains the composite nano-fiber membrane that fiber diameter is 233 nanometers by 1.2 milliliters/hour.
Claims (3)
1. the method for preparing of a fibroin albumen/P (LLA-CL) complex nanometer fibrous tissue renovation bracket comprises:
(1) will remove pupa Bombyx bombycis is 0.5%Na 100 ℃ mass percent
2CO
3Boil 3-5 time in the aqueous solution, each 30min-50min, fully clean with distilled water, in 45 ℃ of-48 ℃ of oven dry, it is 1: 2: 8 CaCl that the silk fiber after the oven dry is dissolved in mol ratio with bath raio 1: 10-15
2, C
2H
5OH and H
2The ternary solvent of O in 60 ℃ of-70 ℃ of constant temperature hydrolysis 1-2h, is used distill water dialysis 72h then, puts into again-(70-80) ℃ precooling 12-18h, and then in-(45-58) ℃ lyophilization, the cellular solid filament fibroin that obtains loosening;
(2) step (1) fibroin albumen is dissolved in hexafluoroisopropanol, is stirred to dissolving fully under the room temperature, obtain the silk fibroin protein solution that concentration is 4-10%g/ml;
(3) P (LLA-CL) is dissolved in hexafluoroisopropanol, is stirred to dissolving fully under the room temperature, obtain the P that concentration is 4-10%g/ml (LLA-CL) solution;
(4) silk fibroin protein solution and step (3) P (LLA-CL) solution are mixed; Wherein fibroin albumen accounts for the 10%-90% of solute gross mass, and P (LLA-CL) accounts for the 90%-10% of solute gross mass, and the mass and size percentage ratio that total soluble matters accounts for solvent is 4-10%; Stir, obtain blend electrostatic spinning liquid;
(5) step (4) blend electrostatic spinning liquid is carried out electrostatic spinning; Adjustment electrostatic spinning process parameter, control voltage is the 8-15 kilovolt, electric field distance is the 60-200 millimeter; Spinning speed be the 0.5-1.5 milliliter/hour, obtain fibroin albumen/P (LLA-CL) composite nano fiber;
P (LLA-CL) in the said step (3) is that mol ratio is 50: 50 the polylactic acid and the random copolymer of polycaprolactone.
2. the method for preparing of a kind of fibroin albumen/P according to claim 1 (LLA-CL) complex nanometer fibrous tissue renovation bracket is characterized in that: said step (4) solute is fibroin albumen and P (LLA-CL).
3. the method for preparing of a kind of fibroin albumen/P according to claim 1 (LLA-CL) complex nanometer fibrous tissue renovation bracket; It is characterized in that: the diameter of said step (5) fibroin albumen/P (LLA-CL) composite nano fiber increases gradually with spinning solution concentration and increases, and reduces gradually with the increase of fibroin albumen content.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101053670A (en) * | 2007-04-03 | 2007-10-17 | 浙江理工大学 | Silk fibroin and polymeric lactic acid compound film and its preparation method |
| CN101264343A (en) * | 2008-05-08 | 2008-09-17 | 上海交通大学 | Silk fiber reinforced polycaprolactone porous scaffold and preparation thereof |
| EP1987850A1 (en) * | 2000-12-21 | 2008-11-05 | Ethicon, Incorporated | Reinforced tissue implants and methods of manufacture and use |
-
2009
- 2009-02-05 CN CN 200910045769 patent/CN101502671B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1987850A1 (en) * | 2000-12-21 | 2008-11-05 | Ethicon, Incorporated | Reinforced tissue implants and methods of manufacture and use |
| CN101053670A (en) * | 2007-04-03 | 2007-10-17 | 浙江理工大学 | Silk fibroin and polymeric lactic acid compound film and its preparation method |
| CN101264343A (en) * | 2008-05-08 | 2008-09-17 | 上海交通大学 | Silk fiber reinforced polycaprolactone porous scaffold and preparation thereof |
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