CN110358663A - A kind of micropore board mount and preparation method thereof - Google Patents
A kind of micropore board mount and preparation method thereof Download PDFInfo
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- CN110358663A CN110358663A CN201810311773.XA CN201810311773A CN110358663A CN 110358663 A CN110358663 A CN 110358663A CN 201810311773 A CN201810311773 A CN 201810311773A CN 110358663 A CN110358663 A CN 110358663A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 23
- 238000005094 computer simulation Methods 0.000 claims description 72
- 239000000463 material Substances 0.000 claims description 37
- 229920000747 poly(lactic acid) Polymers 0.000 claims description 29
- 239000004626 polylactic acid Substances 0.000 claims description 29
- 238000004519 manufacturing process Methods 0.000 claims description 24
- 238000000034 method Methods 0.000 claims description 18
- 239000003086 colorant Substances 0.000 claims description 10
- 238000010146 3D printing Methods 0.000 claims description 8
- 239000011159 matrix material Substances 0.000 claims description 6
- 239000000356 contaminant Substances 0.000 abstract description 5
- 238000002474 experimental method Methods 0.000 description 12
- 238000007639 printing Methods 0.000 description 8
- 230000000694 effects Effects 0.000 description 7
- 238000013461 design Methods 0.000 description 6
- 238000010276 construction Methods 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 238000011160 research Methods 0.000 description 4
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- 238000009825 accumulation Methods 0.000 description 3
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- 230000007812 deficiency Effects 0.000 description 3
- 230000009977 dual effect Effects 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 210000000707 wrist Anatomy 0.000 description 3
- 208000003464 asthenopia Diseases 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 229920002492 poly(sulfone) Polymers 0.000 description 2
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- 239000002994 raw material Substances 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 206010006784 Burning sensation Diseases 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005266 casting Methods 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
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- 238000001514 detection method Methods 0.000 description 1
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- 238000005242 forging Methods 0.000 description 1
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- 239000004417 polycarbonate Substances 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 229920006389 polyphenyl polymer Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/12—Well or multiwell plates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- Life Sciences & Earth Sciences (AREA)
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Abstract
The invention discloses a kind of micropore board mounts and preparation method thereof.The micropore board mount includes: the upper plate for being provided with shrinkage pool, for carrying microwell plate；It is provided with the lower plate of height gear bracket, the upper plate is used to support, is had and the matched hole of upper plate shrinkage pool on height gear bracket；Pin shaft is mounted on different hole and shrinkage pool, so that the adjustable angle between upper plate and lower plate.Micropore board mount of the present invention facilitates the use of microwell plate, can make the gradient of operator's unrestricted choice microwell plate, it is not necessary to continue the angle with hand adjustment section microwell plate, alleviate the operation fatigue of user；It avoids and continues with hand adjustment section microwell plate gradient bring living contaminants.
Description
Technical field
The present invention relates to technical field of experiment equipment, in particular to a kind of micropore board mount and preparation method thereof.
Background technique
Microwell plate is common experiment equipment in life science and medical research, is widely used in cell culture, immunology
The kinds of experiments process such as detection and monoclonal antibody preparation.The manufacture material of microwell plate is usually the pure polyphenyl second of optical clear
Dilute, by the close ordered arrangement of multirow × multiple row cylindrical holes, marking on the orifice surface of the first row and first row has
Word and the alphabetical position to help laboratory technician's resolving aperture.The microwell plate as made of pure polystyrene material is transparent and reflective, and
And repeatedly addition or draw solution in microwell plate are usually needed in same experiment, experiment operator is by naked eyes, it is not easy to
The position of sample-adding is accurately positioned, when transparency liquid is added, it is easier to cause to repeat to add in same hole due to miscount
Sample or leakage plus phenomenon, not only influence the accuracy of experimental result, and bring no small trouble and loss to research work,
It also will cause the unnecessary waste of human and material resources aspect.When handling a large amount of samples, since it is desired that continuing with hand adjustment section microwell plate
Tilt angle, be easy to cause and experimenter's wrist fatigue and bring living contaminants, or even cause experimenter's operation error and influence
Experimental result.Therefore, urgent need one kind can prevent sample-adding mistake, alleviate experimenter behaviour in life science and medical research
Make the micropore board mount of fatigue.And some experiments need to carry out in an aseptic environment, and there are no be suitable for height currently on the market
Press the micropore board mount of sterilized environment.
What is retrieved at present mainly prevents the patented product of the micropore board mount of sample-adding mistake, has following defects that
1, pulley-type: using the sliding of cross slide and perpendicular slide plate, load position is marked, such as grant number is CN
Scheme disclosed in the Chinese patent application file of 204649759 U of 202700547 U and CN, deficiency are to need experimenter manual
Operation increases workload, reduces working efficiency, complicated for operation, is easy to happen cross contamination between sample.
2, groove type: being fixed template using groove, and auxiliary sample-adding ruler is pushed to be marked, such as grant number is CN
Scheme disclosed in the Chinese patent application file of 204724182 U, deficiency are to need experimenter's manual operation to push sample-adding ruler,
Without the function of fixing and adjust tilt angle, it is unfavorable for experimenter's sampling.
3, it is loaded prompting device version: corresponding LED light group, front setting and 96 orifice plates is set using the back side of 96 orifice plates
Every a line and the corresponding several select buttons of each column, controller is set, such as grant number is in 205958592 U of CN
Scheme disclosed in state's patent application document, deficiency operates more complicated, larger workload for setting, and is easy to appear burst feelings
Condition, such as light bulb break down, and also result in the use waste of resource of electric power.
4, a kind of product is only suitable for a kind of specification of orifice plate, and applicable elements are single.
5, it is not suitable for the sterilized environment of high temperature and pressure.
Summary of the invention
In view of the above problems, it proposes on the present invention overcomes the above problem or at least be partially solved in order to provide one kind
State a kind of micropore board mount and preparation method thereof of problem.
In a first aspect, the embodiment of the present invention provides a kind of micropore board mount, comprising: upper plate, lower plate and pin shaft；
The upper plate is provided with shrinkage pool, and the upper plate is for carrying microwell plate；
The lower plate is provided with height gear bracket, has and the matched hole of the shrinkage pool, institute on height gear bracket
It states lower plate and is used to support the upper plate；
The pin shaft is mounted on different described hole and the shrinkage pool, so that the angle between the upper plate and the lower plate
It spends adjustable.
In some alternative embodiments, the shrinkage pool is facing each other is arranged in two opposite sides of the upper plate；
Two be arranged in lower plate the height gear bracket is located at the outside of described two sides, two height
Hole on degree gear bracket is oppositely arranged two-by-two, and described hole matches use with the shrinkage pool.
In some alternative embodiments, rotary shaft is provided on a side of the upper plate far from the shrinkage pool；
The groove of the embeddable rotary shaft is provided in the lower plate；
The rotary shaft is embedded in the groove, and the pin shaft is being mounted on to different described hole and shrinkage pool adjusting
When angle between the upper plate and the lower plate, rotary shaft can be rotated relative to the groove.
In some alternative embodiments, the upper plate be provided with three of the shrinkage pool and the rotary shaft while while
Edge is provided with bar shaped cuboid baffle.
In some alternative embodiments, the shape and size of the upper plate and the lower plate and the microwell plate phase
Match.
In some alternative embodiments, the upper surface of the upper plate has hole corresponding with the hole of the microwell plate
Lattice.
In some alternative embodiments, the upper surface of the upper plate includes at least two color regions, each face
Color region includes at least one cell, and the cell in adjacent color region is coated with different colors.
In some alternative embodiments, the pin shaft is Step Shaft, at least one section and the shrinkage pool on the Step Shaft
Size match, at least one section matches with the size of described hole.
In some alternative embodiments, the microwell plate timbering material is polylactic acid PLA.
Second aspect, the embodiment of the present invention provide a kind of micropore board mount preparation method, comprise the following steps that
Production is provided with the upper plate computer model of shrinkage pool；
Production is provided with the lower plate computer model of height gear bracket, on the height gear bracket of lower plate computer model
With with the matched hole of the shrinkage pool；
Make pin shaft computer model；
Print parameters: printed material filling rate, print temperature, hott bed temperature and print speed are set；
According to the upper plate computer model, lower plate computer model and pin shaft computer model, using 3D printing technique, according to institute
It states print parameters and prints the micropore board mount comprising upper plate, lower plate and pin shaft, the pin shaft may be mounted at different
Described hole and the shrinkage pool, so that the adjustable angle between the upper plate and the lower plate.
In some alternative embodiments, the upper plate computer model, the production lower plate computer model are made, comprising:
Make the shrinkage pool upper plate computer model facing each other that two opposite sides are set；
It is described to make the lower plate computer model for being provided with height gear bracket, comprising: production lower plate matrix model, in lower plate
Two height gear brackets for being located at the outside of described two sides are set on matrix model, in two height
Hole facing each other is set on gear bracket, obtains lower plate computer model；Described hole matches use with the shrinkage pool.
In some alternative embodiments, rotation is arranged in a side in the upper plate computer model far from the shrinkage pool
Axis；
The groove of the embeddable rotary shaft is set on the lower plate computer model；
The rotary shaft is embedded in the groove, and the pin shaft is being mounted on to different described hole and shrinkage pool adjusting
When angle between the upper plate and the lower plate, rotary shaft can be rotated relative to the groove.
In some alternative embodiments, the upper plate computer model is provided with the three of the shrinkage pool and the rotary shaft
Bar shaped cuboid baffle is arranged in the edge on a side.
In some alternative embodiments, the shape and ruler of the upper plate computer model and the lower plate computer model are made
It is very little to match with the micropore board mount microwell plate to be carried.
In some alternative embodiments, in the upper surface of the upper plate computer model, production has and the microwell plate branch
The corresponding cell in hole of the frame microwell plate to be carried.
In some alternative embodiments, it is Step Shaft computer model, the Step Shaft that the pin shaft computer model, which is arranged,
An at least Duan Yuyu described hole for computer model matches, and at least one section matches with described hole.
In some alternative embodiments, the printed material is polylactic acid PLA.
In some alternative embodiments, the filling rate of the PLA is set as 10%~40%.
In some alternative embodiments, the print temperature is set as 190 DEG C~220 DEG C.
In some alternative embodiments, the hott bed temperature setting is 20 DEG C~60 DEG C.
In some alternative embodiments, according to the division principle of setting, the cell of the upper plate upper surface is divided into
At least two regions, each region include at least one cell；
According to the scheme of tinting of setting, the cell of adjacent area is painted to different colors.
The beneficial effect of above-mentioned technical proposal provided in an embodiment of the present invention includes at least:
1, in above-mentioned micropore board mount provided in an embodiment of the present invention and preparation method thereof, by pin shaft by upper plate and lower plate
Adjustable-angle connection, facilitates the gradient of operator's unrestricted choice microwell plate, it is not necessary to continue with hand adjustment section microwell plate
Angle is observed when facilitating sample-adding, alleviates the operation fatigue of user；It avoids and continues with hand adjustment section microwell plate gradient
And bring living contaminants；And help to reduce liquid residual when sucking liquid in experimentation.
2, in above-mentioned micropore board mount provided in an embodiment of the present invention and preparation method thereof, pin shaft passes through lower plate height gear
The corresponding shrinkage pool of different holes and upper plate of bracket, while realizing that upper plate is connect with lower plate adjustable-angle, the rotation of upper plate
The upper plate and lower plate that the groove of axis insertion lower plate makes reach dual connection, to constitute the construction of triangle so that micropore of the present invention
Board mount is more firm.
3, in above-mentioned micropore board mount provided in an embodiment of the present invention and preparation method thereof, upper plate with lower plate is separable sets
Meter allow when a part damage when replace this part after continue to use, material can be saved and improve the present embodiment microwell plate branch
The service efficiency of frame；Meanwhile replacing different upper plates and being applicable to the microwell plate that hole count is different, construction is different, improve this
The scope of application of invention micropore board mount.
4, in above-mentioned micropore board mount provided in an embodiment of the present invention and preparation method thereof, the cell of upper plate upper surface can be with
It is coated with different color regions, help scientific research personnel is by the hole of the color differentiation microwell plate of cell, and reduction mistake adds or leakage adds
The probability of sample alleviates eye fatigue brought by the intense strain and long-time operation when user is loaded.
5, in above-mentioned micropore board mount preparation method provided in an embodiment of the present invention, with polylactic acid (Polylactic
Acid, PLA) it is raw material, pollution-free, fusing point is high, can use under the sterilized environment of high temperature and pressure.
6, it in above-mentioned micropore board mount preparation method provided in an embodiment of the present invention, is manufactured, is simplified by 3D printing technique
Preparation process is integrally formed；Material is saved, precision is high；And it is original strong, support user's personalized customization.
7, it in above-mentioned micropore board mount preparation method provided in an embodiment of the present invention, by a large amount of comparative test, obtains
The print parameters combination of filling rate the most suitable, print temperature and hott bed temperature, the setting of reasonable filling rate, so that system
Standby micropore board mount had not only met stable requirement, but also was unlikely to feel too heavy；The setting of reasonable print temperature, so that
Printing head wire vent is smooth in print procedure, and the actual quality printed is more fine and smooth, while not easily causing printing head again
Plug accident；The setting of reasonable hott bed temperature, can both greatly reduce in print procedure and print failure caused by causing alice,
It is avoided that hot high bed temperature bring is dangerous again.
Other features and advantages of the present invention will be illustrated in the following description, also, partly becomes from specification
It obtains it is clear that understand through the implementation of the invention.The objectives and other advantages of the invention can be by written explanation
Specifically noted structure is achieved and obtained in book, claims and attached drawing.
Below by drawings and examples, technical scheme of the present invention will be described in further detail.
Detailed description of the invention
Attached drawing is used to provide further understanding of the present invention, and constitutes part of specification, with reality of the invention
It applies example to be used to explain the present invention together, not be construed as limiting the invention.In the accompanying drawings:
Fig. 1 is the overall construction exemplary diagram of micropore board mount in the embodiment of the present invention one；
Fig. 2 is the upper plate structure exemplary diagram of micropore board mount in the embodiment of the present invention one；
Fig. 3 is the lower plate structure exemplary diagram of micropore board mount in the embodiment of the present invention one；
Fig. 4 is that the pin shaft of micropore board mount in the embodiment of the present invention one constructs exemplary diagram；
Fig. 5 is the flow example figure of micropore board mount preparation method in the embodiment of the present invention two.
Specific embodiment
Exemplary embodiments of the present disclosure are described in more detail below with reference to accompanying drawings.Although showing the disclosure in attached drawing
Exemplary embodiment, it being understood, however, that may be realized in various forms the disclosure without should be by embodiments set forth here
It is limited.On the contrary, these embodiments are provided to facilitate a more thoroughly understanding of the present invention, and can be by the scope of the present disclosure
It is fully disclosed to those skilled in the art.
It is existing in the prior art during the experiment using the lasting hand adjustment section micropore of needs when microwell plate in order to solve
The tilt angle of plate is easy to cause experimenter's wrist fatigue, easily causes operation error and brings living contaminants；Simultaneously because micro-
Orifice plate is transparent and reflective and leads to experimenter's visual fatigue, causes the problem of wrong adduction leakage sample-adding product are to influence experimental precision,
The embodiment of the present invention provides a kind of micropore board mount, can freely adjust the tilt angle of microwell plate, it is long-term to avoid experimenter
Continue with the tilt angle of hand adjustment section microwell plate, while there is a cell corresponding with the hole of microwell plate in upper plate upper surface, cell can be with
It is coated with regions of different colours, enables experimenter that the position for needing to be loaded is accurately positioned.
Embodiment one
The embodiment of the present invention one provides a kind of micropore board mount, comprising: upper plate 1, lower plate 2 and pin shaft 3.The bracket is suitable for
The suitable microwell plate of all specifications such as 96 orifice plates, 24 orifice plates, 12 orifice plates, 6 orifice plates.Its overall structure can be as shown in Figure 1, scheming
The structural map of 2 upper plates 1 separated for micropore board mount shown in FIG. 1, Fig. 3 micropore board mount shown in FIG. 1 separate
The structural map of lower plate 2, Fig. 4 are the structural map for the pin shaft 3 that micropore board mount shown in FIG. 1 separates.
For upper plate 1 for carrying microwell plate, lower plate 2 is used to support upper plate 1, and adjustable angle connects between upper plate 1 and lower plate 2.
Specifically, it can be upper plate 1 and be provided with shrinkage pool 11, lower plate 2 is provided with height gear bracket 21, band on height gear bracket 21
Have with the matched hole 211 of shrinkage pool 11, pin shaft 3 is mounted on different hole 211 and shrinkage pool 11, so that between upper plate 1 and lower plate 2
Adjustable angle.
Hole 211 and shrinkage pool 11 match, and can be, and quantity, shape, size and position including the two match.
The embodiment of the present invention is connected upper plate 1 with 2 adjustable-angle of lower plate by pin shaft 3, facilitates operator's freedom
Select the gradient of microwell plate, it is not necessary to continue the angle with hand adjustment section microwell plate, be observed when facilitating sample-adding, alleviate use
The operation fatigue of person；It avoids and continues with hand adjustment section microwell plate gradient bring living contaminants；And facilitate experimentation
Liquid residual is reduced when middle sucking liquid.
Meanwhile the separable design of upper plate 1 and lower plate 2 allow replace this part when a part is damaged after continue to make
With, the service efficiency of the present embodiment micropore board mount can be improved, while achieve the purpose that save material；Moreover, replacement is different
Upper plate be to be applicable to the microwell plate that hole count is different, construction is different, improve the scope of application of micropore board mount of the present invention.
It as an optional mode of the present embodiment, can be, shrinkage pool 11 is facing each other, and that upper plate 1 is arranged in is opposite
Two sides can be set in the side of its opposite short side, also can be set in the side of its opposite long side.
Optionally, two be arranged in lower plate 2 the height gear bracket 21 is located at and the described two sides of upper plate
Outside, the hole 211 on two height gear brackets 21 is oppositely arranged two-by-two, and hole 211 matches use with shrinkage pool 11.
Optionally, the position of above-mentioned shrinkage pool 11 and height gear bracket 21 may be set to be other matched positions, only
It can satisfy hole and shrinkage pool that pin shaft may be mounted on different height gear brackets, so that between upper plate and lower plate
Adjustable angle.
As an optional mode of the present embodiment, the quantity of shrinkage pool 11 and hole 211 can be according to microwell plate angle
The demand of gear is adjusted to be arranged, how many a and matched shrinkage pool 11 of hole 211 side of upper plate 1 is arranged, and upper plate 1 is just
The gear of how many grade angle adjustable.
Preferably, the shape of shrinkage pool 11 can be set to cylinder, and hole 211 is shaped to diameter and shrinkage pool
11 diameter is cylindrical accordingly, while the shape of pin shaft 3 and diameter also match with the two.Cylindrical design
For the stationary phase of the upper plate 1 and lower plate 2 that make to simple to operation, pin shaft 3 can be inserted into cylinder without rotating adjusting position at any time
Shrinkage pool 11 and hole 211, and fixing-stable is flexible.Optionally, shrinkage pool 11, hole 211 and pin shaft 3 may be set to be and match
Other shapes.
As an optional mode of the present embodiment, upper plate 1 is provided with rotary shaft on a side far from shrinkage pool 11
13；The groove 22 of embeddable rotary shaft 13 is provided in lower plate 2；The rotary shaft 13 is embedded in the groove 22, pacifies by pin shaft 3
When adjusting the angle between upper plate 1 and lower plate 2 mounted in different holes 211 and shrinkage pool 11, rotary shaft can the relatively described groove rotation
Turn, so that the installation of pin shaft is more convenient.After pin shaft is installed, upper plate is remained unchanged with respect to the tilt angle of lower plate, and is revolved
Shaft is stuck in groove and immobilizes.
Pin shaft 3 in the present embodiment passes through the shrinkage pool of hole 211 and upper plate on opposite lower plate height gear bracket 21
11 upper plates 1 made are connected with 2 adjustable-angle of lower plate, while the groove 22 of the insertion lower plate of the rotary shaft 13 of upper plate makes
Upper plate 1 connected with 2 rotary type of lower plate, the dual construction for connecting and composing triangle, so that the present embodiment micropore board mount exists
It is more firm while freely adjustable gradient.
The shape and size of groove 22 match with rotary shaft 13, and optionally, rotary shaft 13 can be 3/4ths cylinders
Shape, then groove 22 is set as semi-cylindrical, designs the two diameter and length by calculating, so that the rotary shaft 13 of upper plate 1 is lucky
The groove 22 of lower plate 2 can be embedded in and can be freely rotated, upper plate 1 and lower plate 2 is allowed to connect rear upper plate 1 relative to lower plate
2 adjustable angles.Cylindrical design keeps the rotation of rotary shaft 13 more convenient, smooth.Optionally, rotary shaft 13 and groove 22 can also be with
For other corresponding shapes.
By calculating the position of design hole 211 and shrinkage pool 11, the rotary shaft 13 of the upper plate made is embedded in the described recessed of lower plate
While slot 22, each hole 211 on height gear bracket 21, by rotating upper plate 1, can find corresponding can be protected
The shrinkage pool 11 that card pin shaft 3 passes through simultaneously.
As an optional mode of the present embodiment, upper plate 1 is being provided with three sides of shrinkage pool 11 and rotary shaft 13
Edge is provided with bar shaped cuboid baffle 12, and the microwell plate for preventing upper plate 1 from being carried surprisingly slides.
As an optional mode of the present embodiment, the shape and size of upper plate 1 and lower plate 2 to be carried with upper plate 1
Microwell plate match, the size of upper plate 1 should provide place microwell plate needed for space, consider microwell plate place and took out
The convenience operated in journey；Again in line with material, space-saving purpose is saved, it is excessive to be unable to size design.Optionally, upper plate
1 and the shape of lower plate 2 can be set to cuboid, may be set to be other shapes.
As an optional mode of the present embodiment, the upper surface of upper plate 1 has the hole with the microwell plate to be carried
Corresponding cell 14；Cell 14 can be coated with corresponding color, be used to help confirm the position in the hole of carried microwell plate
It sets.
The arrangement mode of cell 14 matches with the arrangement mode in the hole of microwell plate, shapes and sizes also with microwell plate
Hole matches, each hole corresponds to a cell 14 just when the microwell plate made is put into.
As an optional mode of the present embodiment, the upper surface of the upper plate of the present embodiment micropore board mount includes at least
Two color regions, each color region include at least one cell, and the cell in adjacent color region is coated with different face
Color.Facilitate operator to distinguish sample area, judge load position, reduces the wrong probability added or leak sample-adding product, and can help
It helps operator to alleviate eyes and wrist fatigue brought by long-time operation, interest can also be enhanced, alleviate when being loaded
Intense strain.
Optionally, since first cell of the first row first row, 4 adjacent rows × 3 column, 12 cells are coated with phase
Same color, constitutes a color region.Two adjacent field color differences, can be adjacent color lump and do not tint respectively
Be coated with other colors.
Optionally, it is also possible to other different color region combinations or is coated with different colors.
As an optional mode of the present embodiment, pin shaft 3 can be Step Shaft, on the Step Shaft at least one section with
Shrinkage pool 11 matches, and at least one section matches with hole 211.
The shape and size of pin shaft 3 match with shrinkage pool 11 and hole 211.Specifically, the length and hole of pin shaft 3
211 and shrinkage pool 11 length it is corresponding；The shape phase of the shape of the cross section of pin shaft 3 and the cross section of hole 211 and shrinkage pool 11
Matching.
Optionally, when shrinkage pool 211 and hole 211 are all cylindrical, pin shaft 3 needs to be set as the Step Shaft of multistage cylinder.
Optionally, pin shaft 3 can be structure as shown in Figure 4, be made of the lower prop 32 of elongated upper prop 31 and tubbiness.On
The diameter of column 31 and the diameter of the hole 211 on the 2 height gear bracket 21 of shrinkage pool 11 and lower plate of upper plate 1 match；Upper prop 31
Length and the length of the hole 211 on the 2 height gear bracket 21 of shrinkage pool 11 and lower plate of upper plate 1 match.Made by calculating
The upper prop 31 of pin shaft 3 can just pass through the hole 211 of lower plate and the shrinkage pool 11 of upper plate, by 2 adjustable angle of upper plate 1 and lower plate
Formula connection.The diameter of lower prop 32 is greater than the diameter of upper prop 31, so that upper prop 31 passes through the hole 211 of lower plate and the shrinkage pool 11 of upper plate
Shi Buhui falls off outward.
Optionally, pin shaft 3 may be set to be the upper prop 31 of only above-mentioned pin shaft, upper prop 31 of the length than above-mentioned pin shaft
It is slightly longer.
Optionally, pin shaft 3 may be set to be 3 sections and 3 sections or more diameter it is different cylindrical body composition.For example, pin shaft 3
One end diameter match with 11 diameter of shrinkage pool, the diameter phase of the middle part of pin shaft 3 at least one section of step shaft diameter and hole 211
Match, other end diameter is greater than the diameter of hole 211.And/or
One end diameter of pin shaft 3 matches with 11 diameter of shrinkage pool, and other end diameter and the diameter of hole 211 match.
Optionally, pin shaft 3, the shrinkage pool 11 of upper plate 1 are also possible to other corresponding shapes with the hole 211 of lower plate 2.
The quantity of pin shaft 3 is at least 2, and it is next spare can also to prepare more.
As an optional mode of the present embodiment, the material of the present embodiment micropore board mount can be polylactic acid
(Polylactic acid, PLA).
Embodiment two
Second embodiment of the present invention provides a kind of micropore board mount preparation methods, as shown in figure 5, specific preparation process is as follows:
Step S1: production is provided with the upper plate computer model of shrinkage pool.
By the specific configuration of upper plate shown in Fig. 2 in above-described embodiment one, 3D modeling software development is provided with shrinkage pool
Upper plate computer model, the specific steps are as follows:
The shrinkage pool upper plate computer model facing each other that two opposite sides are set is made, is set respectively in each side
Set 4 equal-sized cylindrical shrinkage pools, the shape and size of the shape and size of upper surface and the microwell plate to be carried
Match, optionally, upper plate computer model can be cuboid；In a side of the upper plate computer model far from the shrinkage pool
3/4ths cylindrical rotary shafts are set；Upper plate computer model is provided with three sides of the shrinkage pool and the rotary shaft
Bar shaped cuboid baffle is arranged in edge；In the upper surface of upper plate computer model, production is micro- with to be carried with micropore board mount
The corresponding cell in the hole of orifice plate.
Above-mentioned steps do not have sequencing.The upper plate computer model made is put into and repairs in software see whether it is wrong
Accidentally, it if wrong, modifies in software, until obtaining accurate upper plate computer model.
Step S2: production is provided with the lower plate computer model of height gear bracket, the height gear branch of lower plate computer model
It is had and the matched hole of shrinkage pool on frame.
By the specific configuration of lower plate shown in Fig. 3 in above-described embodiment one, production is made with 3D modeling software and is provided with
The lower plate computer model of height gear bracket, on the height gear bracket of lower plate computer model with the matched hole of shrinkage pool,
Specific step is as follows:
The lower plate matrix model of cuboid is made, the upper table of upper plate computer model in the size and step S1 of upper surface
The size in face matches；Two height for being located at the outside of two sides described in step S1 are set on lower plate matrix model
Gear bracket is spent, cylindrical cavity facing each other is set on two height gear brackets, obtains lower plate computer model, hole
Quantity, diameter and position and shrinkage pool match, hole matches use with shrinkage pool；Embeddable step is set on lower plate computer model
The groove of the semi-cylindrical of rotary shaft in rapid S1, the diameter and length of groove match with rotary shaft, so that rotary shaft is lucky
Groove can be embedded in and can be freely rotated, i.e., rotary shaft can be embedded in groove, and pin shaft is being mounted on different hole and recessed
When hole adjusts the angle between upper plate and lower plate, rotary shaft can confronting grooves rotation.
Above-mentioned steps do not have sequencing, and each section matches.The lower plate computer model made is put into reparation
See whether it is wrong in software, if wrong, modify in software, until obtaining accurate lower plate computer mould
Type.
Step S3: production pin shaft computer model.
By the specific configuration of pin shaft shown in Fig. 4 in above-described embodiment one, production pin shaft electricity is made with 3D modeling software
Brain model, pin shaft may be mounted at different hole and shrinkage pool, so that the adjustable angle between upper plate and lower plate, specific steps are such as
Under:
Production includes the cylindrical pin shaft computer model of upper prop and lower prop, the diameter phase of the diameter and shrinkage pool and hole of upper prop
Matching, the length of upper prop and the length of shrinkage pool and hole match, and the upper prop of the pin shaft made can just pass through the hole of lower plate
With the shrinkage pool of upper plate, upper plate is connected with lower plate adjustable-angle.The diameter of lower prop is greater than the diameter of upper prop, so that upper prop is being worn
More hole and when shrinkage pool will not fall off outward.
The pin shaft computer model made is put into and repairs in software see whether it is wrong, if wrong, in software
It modifies, until obtaining accurate pin shaft computer model.
Above-mentioned pin shaft computer model is also possible to other Step Shaft computer models, and at least one section of Step Shaft computer model
Match with the shrinkage pool with upper plate, the hole of at least one section and lower plate height gear bracket matches.
Step S1~step S3 sequentially, can first carry out any of them one-step or two-step, can also hold simultaneously in no particular order
Row.The specific structure of its upper plate computer model made, lower plate computer model and pin shaft computer model is also not limited to institute as above
The structure stated, as long as meeting the restriction in embodiment one, three's structure matches.
Step S4: setting print parameters: printed material filling rate, print temperature, hott bed temperature and print speed.
The computer model that above-mentioned steps are obtained is put into Slice Software, and print parameters are arranged: can for example fill PLA
Rate is set as 20%, and print temperature is set as 200 DEG C, and hott bed temperature is set as 40 DEG C, and print speed is set as 50mm/s.
The print parameters of above-mentioned computer model and setting are saved in the SD card of 3D printer.
Step S5: it using PLA as printed material, according to upper plate computer model, lower plate computer model and pin shaft computer model, adopts
With 3D printing technique, the micropore board mount comprising upper plate, lower plate and pin shaft is printed according to above-mentioned print parameters.
Pin shaft may be mounted at different hole and shrinkage pool, so that the adjustable angle between upper plate and lower plate；It rotates simultaneously
Axis can be embedded in groove, and the upper plate made can be rotated with confronting grooves.To form the dual company of triangle between upper plate and lower plate
It connects, so that the micropore board mount of the present embodiment is more firm.
Step S6: according to the division principle of setting, the cell of upper plate upper surface is divided at least two regions, Mei Gequ
Domain includes at least one cell；According to the scheme of tinting of setting, the cell of adjacent area is painted to different colors.
According to the actual situation, and/or according to the sample of subscriber individual arrangement habit and Loading sequence it is accustomed to and likes, if
Determine division principle and scheme of tinting, the cell of upper plate upper surface is divided at least two regions, the cell of adjacent area is applied
Upper different color.
Optionally, since first cell of the first row first row, 4 adjacent rows × 3 column, 12 cells are divided into
One region；Different colors is coated in two adjacent regions, be can be adjacent region and is not tinted and coat respectively other face
Color is distinguish.
Optionally, other different demarcation principles can also be selected and scheme of tinting.
As an optional mode of the present embodiment, above-mentioned micropore board mount can be using PLA as raw material, 3D printing system
It makes.PLA is a kind of novel biology base and recyclable organism degradable material, and pollution-free, fusing point is high, can be in the sterilizing of high temperature and pressure
It is used under environment.
Traditional manufacturing technology is typically all to form blank by the mold of blanking or manufacture auxiliary, is then carried out again big
The subsequent mechanical machining of amount finally meets practical size requirement.From various sectional material blanks or casting forging blank to finished product,
Miscellaneous manufacturing process can be used, but basic principle is all removal material to obtain size requirement, referred to as " subtracts material manufacture ".
It will cause the waste on material in this way, can also generate a large amount of waste material, moreover, precision is poor.
And the embodiment of the present invention uses 3D printing technique, with the measure for increasing material accumulation, is required according to final size at one piece
The accumulation of material is carried out on substrate, the part of accumulation molding directly uses or the surface of forming part carries out micro processing, mentions
It can be used after high surface finish.Compared to conventional fabrication processes, 3D printing is had the advantage that
1, material overall utilization is high；
2, without die sinking, manufacturing process is few, and the period is short；
3, the part of labyrinth can be manufactured, such as internal profile-followed runner；
4, it is freely designed according to mechanical property requirements, does not have to consider manufacturing process；
5, Product Precision is high, personalized strong, applied widely.
In order to improve the performance of micropore board mount of the present invention as far as possible while controlling cost, the present invention is in microwell plate
A large amount of comparative test has been done in terms of the printed material and print parameters of bracket.
Experimental example 1
Influence of the material to properties of product.
Have chosen a large amount of material: metal material, common plastic material, ABS material, polycarbonate material, polysulfones PSU
Material and polylactic acid PLA material.Compare the performances such as hardness, quality, heat resistance, crushing resistance and the feature of environmental protection of above-mentioned material, tests
Comparing result is as shown in table 1:
1 a variety of materials performance comparison table of table
By above-mentioned Experimental comparison results, the 3D printing material that polylactic acid PLA material is micropore board mount of the present invention is chosen.
Experimental example 2
Influence of the PLA filling rate to properties of product.
Experimental procedure is first not added toughener in step 4, PLA filling rate is successively set with the step in above-described embodiment two
To be set as 10%, 20%, 30% and 40%, other print parameters are constant, printed respectively using identical printer corresponding
Physical product.10 experimenters are randomly selected as volunteer, everyone is successively set to 10% using 4 kinds of PLA filling rates,
20%, 30% and 40% physical product printed, and count their use feeling.According to the practical impression of volunteer,
The physical product that PLA filling rate prints when being 10%, is not sufficiently stable in use；PLA filling rate is 20% He
The physical model printed when 30%, comfort is higher when use；The entity that PLA filling rate prints when being 40% produces
Product, when use, obviously feel heavy.
It is light as far as possible and save material, reduce cost but simultaneous again simultaneously in line with micropore board mount of the invention is arranged
Using effect is cared for, sufficiently stable principle, the filling rate of PLA of the present invention is preferably 10%~40%, further preferably
20%~30%.
Experimental example 3
Influence of the print parameters-print temperature to properties of product.
Experimental procedure with the step in above-described embodiment two, print temperature is successively set as in step 4 190 DEG C, 200 DEG C,
210 DEG C and 220 DEG C, other print parameters are constant, print corresponding physical product respectively using identical printer.
Pass through observation print procedure and final comparison in kind, when print temperature is 190 DEG C, printer printing head wire vent
It is not smooth enough and in kind more coarse；When print temperature is 200 DEG C, 210 DEG C, wire vent is smooth, better quality；Print temperature is
At 220 DEG C, the easy plug of printing head, surface in kind has burning sensation.
In conclusion final print temperature is preferably 190 DEG C~220 DEG C, further preferably 200 DEG C~210 DEG C.
Experimental example 4
Print parameters-influence of the hott bed temperature to properties of product.
Hott bed temperature is successively set as 10 DEG C, 20 DEG C, 40 in step 4 with the step in above-described embodiment two by experimental procedure
DEG C, 60 DEG C and 70 DEG C, other print parameters are constant, print corresponding physical product respectively using identical printer.
Observation printing situation discovery easily causes alice in print procedure, printing is caused to be lost when hott bed temperature is 10 DEG C
It loses；When hott bed temperature is 20 DEG C, the case where alice, is significantly reduced when compared with hott bed temperature being 10 DEG C in print procedure；When hott bed temperature
When degree is 40 DEG C and 60 DEG C, effect is preferable；Danger is easily caused in print procedure when hott bed temperature is 70 DEG C.
In summary situation, final preferably hott bed temperature is 20 DEG C~60 DEG C, further preferably 40 DEG C~60 DEG C.
Experimental example 5
Influence of the print parameters-print speed to properties of product.
Experimental procedure with the step in above-described embodiment two, in step 4 print speed be set to 20mm/s, 40mm/s,
60mm/s and 80mm/s, other print parameters are constant, print corresponding physical product respectively using identical printer.
Observation printing situation, and compare the effect in kind printed and obtain: print speed is slower, time-consuming longer, material object effect
Fruit is better；Print speed is faster, and time-consuming shorter effect in kind is poorer.
Comprehensive print time and printing effect, final preferably print speed is 40mm/s~60mm/s.
Experimental example 6
The influence of print parameters-print temperature, hott bed temperature and print time Comprehensive Correlation to properties of product.
Experimental procedure with the step in above-described embodiment two, print parameters-print temperature, hott bed temperature in step 4 and
Print speed is respectively set to the different combinations in table 2, prints corresponding physical product respectively using identical printer.
The Contrast on effect in kind that different print parameters combine corresponding print procedure situation and print is as shown in table 2:
The different print parameters of table 2 combine corresponding print procedure and print contrast table in kind
In summary situation, final print temperature are preferably 190 DEG C~220 DEG C, and hott bed temperature is preferably 20 DEG C~60 DEG C,
Print speed is preferably 40mm/s~60mm/s.
In above-mentioned detailed description, various features are combined together in single embodiment, to simplify the disclosure.No
This published method should be construed to reflect such intention, that is, the embodiment of theme claimed needs clear
The more features of the feature stated in each claim to Chu.On the contrary, that reflected such as appended claims
Sample, the present invention are in the state fewer than whole features of disclosed single embodiment.Therefore, appended claims is special
This is expressly incorporated into detailed description, and wherein each claim is used as alone the individual preferred embodiment of the present invention.
Description above includes the citing of one or more embodiments.Certainly, in order to describe above-described embodiment and description portion
The all possible combination of part or method is impossible, but it will be appreciated by one of ordinary skill in the art that each implementation
Example can do further combinations and permutations.Therefore, embodiment described herein is intended to cover fall into the appended claims
Protection scope in all such changes, modifications and variations.In addition, with regard to term used in specification or claims
The mode that covers of "comprising", the word is similar to term " includes ", just as " including " solved in the claims as transitional word
As releasing.In addition, the use of any one of specification in claims term "or" being to indicate " non-exclusionism
Or ".
Claims (11)
1. a kind of micropore board mount, which is characterized in that including upper plate, lower plate and pin shaft；
The upper plate is provided with shrinkage pool, and the upper plate is for carrying microwell plate；
The lower plate is provided with height gear bracket, on height gear bracket with the matched hole of the shrinkage pool, under described
Plate is used to support the upper plate；
The pin shaft is mounted on different described hole and the shrinkage pool, so that the angle between the upper plate and the lower plate can
It adjusts.
2. micropore board mount as described in claim 1, which is characterized in that the shrinkage pool is facing each other to be arranged in the upper plate
Two opposite sides；
Two be arranged in lower plate the height gear bracket is located at the outside of described two sides, two height shelves
Hole on the bracket of position is oppositely arranged two-by-two, and described hole matches use with the shrinkage pool.
3. micropore board mount as described in claim 1, which is characterized in that on a side of the upper plate far from the shrinkage pool
It is provided with rotary shaft；
The groove of the embeddable rotary shaft is provided in the lower plate；
The rotary shaft is embedded in the groove, and the pin shaft is being mounted on described in different described hole and shrinkage pool adjusting
When angle between upper plate and the lower plate, rotary shaft can be rotated relative to the groove.
4. micropore board mount as described in any one of claims 1-3, which is characterized in that the shape of the upper plate and the lower plate
Match with size and the microwell plate；
The upper surface of the upper plate has cell corresponding with the hole of the microwell plate.
5. micropore board mount as claimed in claim 4, which is characterized in that the upper surface of the upper plate includes at least two colors
Region, each color region include at least one cell, and the cell in adjacent color region is coated with different colors.
6. micropore board mount as described in any one of claims 1-3, which is characterized in that the pin shaft be Step Shaft, described
Match with the shrinkage pool at least one section on rank axis, at least one section matches with described hole.
7. a kind of micropore board mount preparation method, comprising steps are as follows:
Production is provided with the upper plate computer model of shrinkage pool；
Production is provided with the lower plate computer model of height gear bracket, has on the height gear bracket of lower plate computer model
With the matched hole of the shrinkage pool；
Make pin shaft computer model；
Print parameters: printed material filling rate, print temperature, hott bed temperature and print speed are set；
It is beaten using 3D printing technique according to described according to the upper plate computer model, lower plate computer model and pin shaft computer model
Print parameter prints the micropore board mount comprising upper plate, lower plate and pin shaft, and the pin shaft may be mounted at different described
Hole and the shrinkage pool, so that the adjustable angle between the upper plate and the lower plate.
8. preparation method as claimed in claim 7, which is characterized in that make the upper plate computer model, the lower plate computer
Model and the pin shaft computer model, comprising:
Make the shrinkage pool upper plate computer model facing each other that two opposite sides are set；
It is described to make the lower plate computer model for being provided with height gear bracket, comprising: production lower plate matrix model, in lower plate matrix
Two height gear brackets for being located at the outside of described two sides are set on model, in two height gears
Hole facing each other is set on bracket, obtains lower plate computer model；Described hole matches use with the shrinkage pool；
In a side of the upper plate computer model far from the shrinkage pool, rotary shaft is set；
The groove of the embeddable rotary shaft is set on the lower plate computer model；
The rotary shaft is embedded in the groove, and the pin shaft is being mounted on described in different described hole and shrinkage pool adjusting
When angle between upper plate and the lower plate, rotary shaft can be rotated relative to the groove；
In the upper surface of the upper plate computer model, production has the hole phase with the micropore board mount microwell plate to be carried
Corresponding cell；
It is Step Shaft computer model that the pin shaft computer model, which is arranged, at least one section of the Step Shaft computer model with it is described
Shrinkage pool matches, and at least one section matches with described hole.
9. preparation method as claimed in claim 7 or 8, which is characterized in that further include:
The printed material is polylactic acid PLA；
The filling rate of the PLA is set as 10%~40%.
10. preparation method as claimed in claim 9, which is characterized in that further include:
The print temperature is set as 190 DEG C~220 DEG C；
The hott bed temperature setting is 20 DEG C~60 DEG C.
11. preparation method as claimed in claim 8, which is characterized in that further include:
According to the division principle of setting, the cell of the upper plate upper surface is divided at least two regions, each region
Including at least one cell；
According to the scheme of tinting of setting, the cell of adjacent area is painted to different colors.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810311773.XA CN110358663A (en) | 2018-04-09 | 2018-04-09 | A kind of micropore board mount and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810311773.XA CN110358663A (en) | 2018-04-09 | 2018-04-09 | A kind of micropore board mount and preparation method thereof |
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