CN107053543B - Suitable for anaerobic resin imbedded mold - Google Patents
Suitable for anaerobic resin imbedded mold Download PDFInfo
- Publication number
- CN107053543B CN107053543B CN201710272940.XA CN201710272940A CN107053543B CN 107053543 B CN107053543 B CN 107053543B CN 201710272940 A CN201710272940 A CN 201710272940A CN 107053543 B CN107053543 B CN 107053543B
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- Prior art keywords
- raised line
- main body
- die main
- solid
- thread hole
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C33/00—Moulds or cores; Details thereof or accessories therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C33/00—Moulds or cores; Details thereof or accessories therefor
- B29C33/0038—Moulds or cores; Details thereof or accessories therefor with sealing means or the like
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C33/00—Moulds or cores; Details thereof or accessories therefor
- B29C33/30—Mounting, exchanging or centering
- B29C33/305—Mounting of moulds or mould support plates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
- G01N2001/364—Embedding or analogous mounting of samples using resins, epoxy
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- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses one kind to be suitable for anaerobic resin imbedded mold, it includes left die main body, right die main body, solid octahedron, top cover, it is solid octahedron two sides respectively with left die main body, right die main body connection, left die main body, right die main body, solid octahedron is all connect with top cover, wherein: left die main body includes eight prism grooves, left raised line, right raised line, left-hand thread hole, right-hand thread hole, left notch, right notch, left raised line, right raised line is all connect with eight prism grooves, left raised line is located on the left of right raised line, left-hand thread hole is located on left raised line, right-hand thread hole is located on right raised line etc..The present invention is easy to putting for sample, and sample will not generate compressional deformation in embedding process, and detachably, the resin-like sheet having polymerize is easy to access, forms an enclosed system, is suitable for anaerobic type resin polymerization；To volume, there is no limit, is applicable to various sizes of biological organization sample embedding, easily operated, is suitble to promote the use of in common laboratory.
Description
Technical field
The present invention relates to a kind of imbedded molds, are suitable for anaerobic resin imbedded mold more particularly to one kind.
Background technique
In order to obtain high-resolution biological tissue's fine structure information, it is higher that biological tissue embedding is entered hardness by researcher
Resin in, prepare tissue ultra-thin section, be used for electron microscope and optical microscopy imaging observation.A kind of anaerobic resin is (such as
GMA, HM20 etc.) extremely excellent characteristic has been shown to the embedding of tissue.However, the polymerizing condition of this kind of resin is but very severe
It carves, needs in polymerization process stringent oxygen barrier, be otherwise unable to reach um slice and require or even can not polymerize.So we are to resin packet
Burying the leakproofness of environment, more stringent requirements are proposed.
In addition, since the size difference of the biological organization sample of different plant species is larger, by taking brain sample as an example, Adult Rat Brain
Having a size of 2.5cm, a height of 2mm*2cm*1.5cm, a height of 2mm, adult rabbits brain having a size of 4.5cm, a height of 2mm*3cm*2.5cm,
A height of 2mm, adult crab eating monkeys monkey brain is having a size of 7cm*4.5cm, a height of 2mm*3cm.Especially for neural circuitry in full intracerebral
Distribution and structure research in, carry out whole embedding and imaging with greater need for entire brain tissue, this gives large volume biological tissue
The embedding techniques of sample bring new challenge.
There are mainly two types of for the imbedded mold generally used at present:
1. the silica gel mould of the bad imbedded mold of leakproofness or the class that is open, is only applicable to the embedding of non-anaerobic type resin.
2. another imbedded mold is capsule shells, capsule shells have certain leakproofness, can be used for polymerizeing anaerobic type resin,
But its volume is extremely limited.With the continuous extension of Research Requirements, our sample also will be gradually from the biology of smaller size
Tissue (such as mouse brain) is transitioned into larger sized biological tissue (such as monkey brain), for having embedded the gelatine capsule of mouse brain
It is not able to satisfy our demand completely.
In addition, to all have geomery again nonconforming for the commercially available preferable packing box of all kinds of leakproofness, and it is not easy to remove
The disadvantages of.
To sum up, current resin embedding mold is primarily present the too small problem of poor sealing or volume, and other
It is nonconforming that substitute products all have geomery again, and is not easy the shortcomings that removing.It is suitable for detesting for this purpose, we devise one kind
Oxygen resin embedding mold, this mold can form an enclosed system, be suitable for anaerobic type resin polymerization；Body of this mold to tissue
There is no limit be applicable to various sizes of biological organization sample embedding to product；This mold is easy to remove, and convenience sample is lossless to be taken
Out.
Summary of the invention
Technical problem to be solved by the invention is to provide one kind to be suitable for anaerobic resin imbedded mold, is easy to sample
It puts, sample will not generate compressional deformation in embedding process, and detachably, the resin-like sheet having polymerize is easy to access, form one
Enclosed system is suitable for anaerobic type resin polymerization；To volume, there is no limit be applicable to various sizes of biological organization sample packet
It buries, it is easily operated, it is suitble to promote the use of in common laboratory.
The present invention is that above-mentioned technical problem is solved by following technical proposals: one kind being suitable for anaerobic resin and embeds mould
Tool comprising left die main body, right die main body, solid octahedra, top cover, solid octahedron two sides respectively with left mold master
Body, the connection of right die main body, left die main body, right die main body, solid octahedron are all connect with top cover, in which:
Left die main body is lacked including eight prism grooves, left raised line, right raised line, left-hand thread hole, right-hand thread hole, left notch, the right side
Mouthful, left raised line, right raised line are all connect with eight prism grooves, and left raised line is located on the left of right raised line, and left-hand thread hole is located on left raised line,
Right-hand thread hole is located on right raised line, and left-hand thread hole is located on the left of right-hand thread hole, and left notch is located on the left of right notch, and left notch is located at
At the top of left raised line, right notch is located at the top of right raised line；
Solid octahedra including left projection, right projection, octagon column, octagon column two sides are connect with left projection, right projection respectively.
Preferably, the solid octahedra bottom surface, which is carved with dimension line, makes mold be conducive to the tissue side of ajusting in use
Position.
Preferably, the cube that two side lengths are 5mm, a height of 2mm is respectively adopted in the left projection, right projection, so that real
Heart octahedron is stuck in eight prism grooves in die main body, forms one with a thickness of 1mm spatial joint clearance.
The positive effect of the present invention is that: the present invention is easy to putting for sample, and sample will not generate in embedding process
Compressional deformation, detachably, the resin-like sheet having polymerize are easy to access, form an enclosed system, and it is poly- to be suitable for anaerobic type resin
It closes；To volume, there is no limit, is applicable to various sizes of biological organization sample embedding, easily operated, is suitble to generally testing
It promotes the use of room.
Detailed description of the invention
Fig. 1 is the structural schematic diagram that the present invention is suitable for anaerobic resin imbedded mold.
Fig. 2 is another structural schematic diagram of the invention.
Fig. 3 is the structural schematic diagram of left die main body in the present invention.
Fig. 4 is solid octahedral structural schematic diagram in the present invention.
Specific embodiment
Present pre-ferred embodiments are provided with reference to the accompanying drawing, in order to explain the technical scheme of the invention in detail.
As shown in Figure 1 and Figure 2, it includes left die main body 1, right die main body that the present invention, which is suitable for anaerobic resin imbedded mold,
2, solid octahedra 3, top cover 4, solid octahedra 3 two sides are connect with left die main body 1, right die main body 2 respectively, left mold master
Body 1, right die main body 2, solid octahedra 3 are all connect with top cover 4, in which:
As shown in figure 3, left die main body 1 includes eight prism grooves 11, left raised line 12, right raised line 13, left-hand thread hole 14, the right side
Threaded hole 15, left notch 16, right notch 17, left raised line 12, right raised line 13 are all connect with eight prism grooves 11, and left raised line 12 is located at
Right 13 left side of raised line, left-hand thread hole 14 are located on left raised line 12, and right-hand thread hole 15 is located on right raised line 13, and left-hand thread hole 14 is located at
15 left side of right-hand thread hole, left notch 16 are located at right 17 left side of notch, and left notch 16 is located at left 12 top of raised line, and right notch 17 is located at
Right 13 top of raised line；
As shown in figure 4, solid octahedra 3 include left projection 31, right projection 32, octagon column 33,33 two sides of octagon column difference
It is connect with left projection 31, right projection 32.
Solid octahedra 3 bottom surface, which is carved with dimension line mold is made to be conducive to tissue in use, ajusts orientation.
The cube that two side lengths are 5mm, a height of 2mm is respectively adopted in left projection 31, right projection 32, so that solid octahedron
3 are stuck in eight prism grooves 11 in die main body, form one with a thickness of 1mm spatial joint clearance.
Specific steps are as follows:
Mold or so two halves main body is combined, screw, nut is tightened in the threaded hole on both sides by step 1, will be whole
A die main body is fixed locked；
Step 2 on the raised line of the right and left that two convex blocks of eight prisms are stuck in die main body, makes die main body
With the spatial joint clearance for forming an about 1mm among eight prisms；
Step 3 is injected the good gelatin solution of predissolve in spatial joint clearance, is taken out after experiment process forms after gelatin
Eight intermediate prisms form the resin embedding mold for being easy to dismounting, good airproof performance.
Particular embodiments described above, the technical issues of to solution of the invention, technical scheme and beneficial effects carry out
It is further described, it should be understood that the above is only a specific embodiment of the present invention, is not limited to
The present invention, all within the spirits and principles of the present invention, any modification, equivalent substitution, improvement and etc. done should be included in this
Within the protection scope of invention.
Claims (3)
1. one kind is suitable for anaerobic resin imbedded mold, which is characterized in that it includes left die main body, right die main body, solid
Octahedra, top cover, solid octahedron two sides are connect with left die main body, right die main body respectively, left die main body, right mould tool master
Body, solid octahedron are all connect with top cover, in which:
Left die main body includes eight prism grooves, left raised line, right raised line, left-hand thread hole, right-hand thread hole, left notch, right notch, a left side
Raised line, right raised line are all connect with eight prism grooves, and left raised line is located on the left of right raised line, and left-hand thread hole is located on left raised line, right spiral shell
Pit is located on right raised line, and left-hand thread hole is located on the left of right-hand thread hole, and left notch is located on the left of right notch, and left notch is located at left convex
At the top of item, right notch is located at the top of right raised line；
Solid octahedra including left projection, right projection, octagon column, octagon column two sides are connect with left projection, right projection respectively.
2. being suitable for anaerobic resin imbedded mold as described in claim 1, which is characterized in that carve the solid octahedra bottom surface
There is dimension line that mold is made to be conducive to tissue in use and ajusts orientation.
3. being suitable for anaerobic resin imbedded mold as described in claim 1, which is characterized in that the left projection, right projection point
Do not use two side lengths for the cube of 5mm, a height of 2mm, so that solid octahedron is stuck in eight prism grooves in die main body
It is interior, one is formed with a thickness of 1mm spatial joint clearance.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710272940.XA CN107053543B (en) | 2017-04-24 | 2017-04-24 | Suitable for anaerobic resin imbedded mold |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710272940.XA CN107053543B (en) | 2017-04-24 | 2017-04-24 | Suitable for anaerobic resin imbedded mold |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107053543A CN107053543A (en) | 2017-08-18 |
| CN107053543B true CN107053543B (en) | 2019-01-04 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710272940.XA Active CN107053543B (en) | 2017-04-24 | 2017-04-24 | Suitable for anaerobic resin imbedded mold |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108389492A (en) * | 2018-04-27 | 2018-08-10 | 佛山拾念科技有限公司 | A kind of dismountable bioconversion medium plant cell mold |
| CN110146366B (en) * | 2019-06-11 | 2022-04-05 | 河南科技学院 | A mould for preparing wax stone for pathological tissue embedding |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102005050320B4 (en) * | 2005-10-20 | 2013-07-04 | Montblanc-Simplo Gmbh | Method for producing a transparent body with an enclosed object |
| KR100829213B1 (en) * | 2006-12-06 | 2008-05-14 | 송승민 | Mold of recipient block and usage thereof |
| US9039600B2 (en) * | 2013-03-12 | 2015-05-26 | Steven A. Shubin, Sr. | Systems and methods related to collection of biological fluids |
| CN205719722U (en) * | 2016-06-21 | 2016-11-23 | 武汉赛维尔生物科技有限公司 | A kind of biological tissue paraffin-embedded mould |
| CN205958337U (en) * | 2016-08-05 | 2017-02-15 | 徐菲菲 | Pathological tissue paraffin embedding mould |
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| Publication number | Publication date |
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| CN107053543A (en) | 2017-08-18 |
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