CN107053543A - Suitable for anaerobic resin imbedded mold - Google Patents
Suitable for anaerobic resin imbedded mold Download PDFInfo
- Publication number
- CN107053543A CN107053543A CN201710272940.XA CN201710272940A CN107053543A CN 107053543 A CN107053543 A CN 107053543A CN 201710272940 A CN201710272940 A CN 201710272940A CN 107053543 A CN107053543 A CN 107053543A
- Authority
- CN
- China
- Prior art keywords
- raised line
- main body
- die main
- solid
- thread hole
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C33/00—Moulds or cores; Details thereof or accessories therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C33/00—Moulds or cores; Details thereof or accessories therefor
- B29C33/0038—Moulds or cores; Details thereof or accessories therefor with sealing means or the like
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C33/00—Moulds or cores; Details thereof or accessories therefor
- B29C33/30—Mounting, exchanging or centering
- B29C33/305—Mounting of moulds or mould support plates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
- G01N2001/364—Embedding or analogous mounting of samples using resins, epoxy
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- Engineering & Computer Science (AREA)
- Mechanical Engineering (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
It is applied to anaerobic resin imbedded mold the invention discloses one kind, it includes left die main body, right die main body, solid octahedra, top cover, solid octahedra both sides are connected with left die main body, right die main body respectively, left die main body, right die main body, solid octahedron are all connected with top cover, wherein：Left die main body includes eight prism grooves, left raised line, right raised line, left-hand thread hole, right-hand thread hole, left breach, right breach, left raised line, right raised line are all connected with eight prism grooves, left raised line is located on the left of right raised line, and left-hand thread hole is located on left raised line, and it is first-class that right-hand thread hole is located at right raised line.The present invention is easy to putting for sample, and sample will not produce compressional deformation in embedding process, detachably, and the resin-like sheet having polymerize is capable of being conveniently and easily taken, forms an enclosed system, it is adaptable to anaerobic type resin polymerization；Volume is not limited, various sizes of biological organization sample embedding is applicable to, it is easy to operate, be adapted to promote the use of in common laboratory.
Description
Technical field
It is more particularly to a kind of to be applied to anaerobic resin imbedded mold the present invention relates to a kind of imbedded mold.
Background technology
In order to obtain high-resolution biological tissue's fine structure information, it is higher that biological tissue embedding is entered hardness by researcher
Resin in, tissue ultra-thin section is prepared, for electron microscope and light microscope imaging observation.One class anaerobic resin is (such as
GMA, HM20 etc.) extremely excellent characteristic has been shown to the embedding of tissue.However, the polymerizing condition of this resinoid is but very severe
Carve, strict oxygen barrier is needed in polymerization process, be otherwise unable to reach um sections and require or even can not polymerize.So we are to resin bag
The sealing for burying environment proposes higher requirement.
Further, since the size difference of the biological organization sample of different plant species is larger, and by taking brain sample as an example, Adult Rat Brain
Size be 2.5cm, a height of 2mm*2cm*1.5cm, a height of 2mm, adult rabbits brain size be 4.5cm, a height of 2mm*3cm*2.5cm,
A height of 2mm, adult crab eating monkeys monkey brain size is 7cm*4.5cm, a height of 2mm*3cm.Especially for neural circuitry in full intracerebral
Distribution and structure research in, carry out overall embedding and imaging with greater need for whole brain tissue, this gives large volume biological tissue
The embedding techniques of sample brings new challenge.
At present generally using to imbedded mold mainly have following two：
1. the not good imbedded mold of sealing or the silica gel mould for the class that is open, are only applicable to the embedding of non-anaerobic type resin.
2. another imbedded mold is capsule shells, and capsule shells have certain sealing, available for polymerization anaerobic type resin,
But its volume is extremely limited.With the continuous extension of Research Requirements, our sample is also by progressively from the biology of reduced size
Tissue (such as mouse brain) is transitioned into larger sized biological tissue (such as monkey brain), for having embedded the gelatine capsule of mouse brain
Through our demand can not be met completely.
In addition, to be respectively provided with geomery again nonconforming for the commercially available preferable packing box of all kinds of sealings, and it is difficult to remove
The shortcomings of.
To sum up, the problem of current resin embedding mould is primarily present poor sealing or too small volume, and it is other
Substitute products are respectively provided with that geomery is nonconforming again, and are difficult the shortcoming removed.It is applied to detest therefore, we devise one kind
Oxygen tree fat imbedded mold, this mould can form an enclosed system, it is adaptable to anaerobic type resin polymerization；Body of this mould to tissue
Product is not limited, and is applicable to various sizes of biological organization sample embedding；This mould is easy to remove, and convenience sample is lossless to be taken
Go out.
The content of the invention
The technical problems to be solved by the invention are to provide one kind and are applied to anaerobic resin imbedded mold, and it is easy to sample
Put, sample will not produce compressional deformation in embedding process, detachably, the resin-like sheet having polymerize is capable of being conveniently and easily taken, form one
Enclosed system, it is adaptable to anaerobic type resin polymerization；Volume is not limited, various sizes of biological organization sample bag is applicable to
Bury, it is easy to operate, be adapted to promote the use of in common laboratory.
The present invention is to solve above-mentioned technical problem by following technical proposals：One kind is applied to anaerobic resin and embeds mould
Tool, it includes left die main body, right die main body, solid octahedra, top cover, and solid octahedra both sides have with left mould respectively leads
Body, the connection of right die main body, left die main body, right die main body, solid octahedron are all connected with top cover, wherein：
Left die main body lacks including eight prism grooves, left raised line, right raised line, left-hand thread hole, right-hand thread hole, left breach, the right side
Mouthful, left raised line, right raised line are all connected with eight prism grooves, and left raised line is located on the left of right raised line, and left-hand thread hole is located on left raised line,
Right-hand thread hole is located on right raised line, and left-hand thread hole position is on the left of right-hand thread hole, and left breach is located on the left of right breach, and left breach is located at
At the top of left raised line, right breach is located at the top of right raised line；
Solid octahedra including left projection, right projection, octagon column, octagon column both sides are connected with left projection, right projection respectively.
Preferably, the solid octahedra bottom surface, which is carved with dimension line, makes mould be conducive to the tissue side of ajusting in use
Position.
Preferably, two length of sides are respectively adopted for 5mm, a height of 2mm cube in the left projection, right projection so that real
Heart octahedron is stuck in eight prism grooves in die main body, and it is 1mm spatial joint clearances to form a thickness.
The positive effect of the present invention is：The present invention is easy to putting for sample, and sample will not be produced in embedding process
Compressional deformation, detachably, the resin-like sheet having polymerize are capable of being conveniently and easily taken, form an enclosed system, it is adaptable to which anaerobic type resin gathers
Close；Volume is not limited, various sizes of biological organization sample embedding is applicable to, it is easy to operate, be adapted to typically testing
Promote the use of room.
Brief description of the drawings
Fig. 1 is applied to a structural representation of anaerobic resin imbedded mold for the present invention.
Fig. 2 is another structural representation of the invention.
Fig. 3 is the structural representation of left die main body in the present invention.
Fig. 4 is solid octahedral structural representation in the present invention.
Embodiment
Present pre-ferred embodiments are provided below in conjunction with the accompanying drawings, to describe technical scheme in detail.
As shown in Figure 1 and Figure 2, the present invention, which is applied to anaerobic resin imbedded mold, includes left die main body 1, right die main body
2nd, solid octahedra 3, top cover 4, solid octahedra 3 both sides are connected with left die main body 1, right die main body 2 respectively, left mould tool master
Body 1, right die main body 2, solid octahedra 3 are all connected with top cover 4, wherein：
As shown in figure 3, left die main body 1 includes eight prism grooves 11, left raised line 12, right raised line 13, left-hand thread hole 14, the right side
Screwed hole 15, left breach 16, right breach 17, left raised line 12, right raised line 13 are all connected with eight prism grooves 11, and left raised line 12 is located at
The right left side of raised line 13, left-hand thread hole 14 is located on left raised line 12, and right-hand thread hole 15 is located on right raised line 13, and left-hand thread hole 14 is located at
The left side of right-hand thread hole 15, left breach 16 is located at the right left side of breach 17, and left breach 16 is located at the left top of raised line 12, and right breach 17 is located at
The right top of raised line 13；
As shown in figure 4, solid octahedra 3 include left projection 31, right projection 32, octagon column 33, the both sides of octagon column 33 difference
It is connected with left projection 31, right projection 32.
Solid octahedra 3 bottom surface, which is carved with dimension line, makes mould be conducive to tissue to ajust orientation in use.
Two length of sides are respectively adopted for 5mm, a height of 2mm cube in left projection 31, right projection 32 so that solid octahedron
4 are stuck in eight prism grooves 5 in die main body 2, and it is 1mm spatial joint clearances to form a thickness.
Concrete operation step is as follows：
Step one, mould or so two halves main body is combined, screw, nut is tightened in the screwed hole on both sides, will be whole
Individual die main body is fixed locked；
Step 2, two projections of eight prisms are stuck on the raised line of the right and left of die main body, make die main body
With the spatial joint clearance that an about 1mm is formed in the middle of eight prisms；
Step 3, the good gelatin solution of predissolve is injected in spatial joint clearance, is taken out after gelatin is molded through experiment process
Eight middle prisms, that is, form one and be easy to dismounting, the resin embedding mould of good airproof performance.
Particular embodiments described above, technical problem, technical scheme and beneficial effect to the solution of the present invention are carried out
It is further described, should be understood that the specific embodiment that the foregoing is only of the invention, be not limited to
The present invention, within the spirit and principles of the invention, any modification, equivalent substitution and improvements done etc. should be included in this
Within the protection domain of invention.
Claims (3)
1. one kind is applied to anaerobic resin imbedded mold, it is characterised in that it includes left die main body, right die main body, solid
Octahedra, top cover, solid octahedra both sides are connected with left die main body, right die main body respectively, left die main body, right mould tool master
Body, solid octahedron are all connected with top cover, wherein：
Left die main body includes eight prism grooves, left raised line, right raised line, left-hand thread hole, right-hand thread hole, left breach, right breach, left
Raised line, right raised line are all connected with eight prism grooves, and left raised line is located on the left of right raised line, and left-hand thread hole is located on left raised line, right spiral shell
Pit is located on right raised line, and left-hand thread hole position is on the left of right-hand thread hole, and left breach is located on the left of right breach, and left breach is located at left convex
At the top of bar, right breach is located at the top of right raised line；
Solid octahedra including left projection, right projection, octagon column, octagon column both sides are connected with left projection, right projection respectively.
2. it is applied to anaerobic resin imbedded mold as claimed in claim 1, it is characterised in that carve the solid octahedra bottom surface
There is dimension line to make mould be conducive to tissue to ajust orientation in use.
3. it is applied to anaerobic resin imbedded mold as claimed in claim 1, it is characterised in that the left projection, right projection point
Two length of sides are not used for 5mm, a height of 2mm cube so that solid octahedron is stuck in eight prism grooves in die main body
Interior, it is 1mm spatial joint clearances to form a thickness.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710272940.XA CN107053543B (en) | 2017-04-24 | 2017-04-24 | Suitable for anaerobic resin imbedded mold |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710272940.XA CN107053543B (en) | 2017-04-24 | 2017-04-24 | Suitable for anaerobic resin imbedded mold |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107053543A true CN107053543A (en) | 2017-08-18 |
| CN107053543B CN107053543B (en) | 2019-01-04 |
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ID=59603501
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710272940.XA Active CN107053543B (en) | 2017-04-24 | 2017-04-24 | Suitable for anaerobic resin imbedded mold |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107053543B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108389492A (en) * | 2018-04-27 | 2018-08-10 | 佛山拾念科技有限公司 | A kind of dismountable bioconversion medium plant cell mold |
| CN110146366A (en) * | 2019-06-11 | 2019-08-20 | 河南科技学院 | A kind of mold being used to prepare pathologic tissue packing wax stone |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102005050320A1 (en) * | 2005-10-20 | 2007-04-26 | Montblanc-Simplo Gmbh | Method for producing a transparent body with an enclosed object |
| JP2010511889A (en) * | 2006-12-06 | 2010-04-15 | スンミン ソン | Recipient block mold and its uses |
| CN104042436A (en) * | 2013-03-12 | 2014-09-17 | 老史蒂文·A·舒宾 | Systems and methods related to collection of biological fluids |
| CN205719722U (en) * | 2016-06-21 | 2016-11-23 | 武汉赛维尔生物科技有限公司 | A kind of biological tissue paraffin-embedded mould |
| CN205958337U (en) * | 2016-08-05 | 2017-02-15 | 徐菲菲 | Pathological tissue paraffin embedding mould |
-
2017
- 2017-04-24 CN CN201710272940.XA patent/CN107053543B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102005050320A1 (en) * | 2005-10-20 | 2007-04-26 | Montblanc-Simplo Gmbh | Method for producing a transparent body with an enclosed object |
| JP2010511889A (en) * | 2006-12-06 | 2010-04-15 | スンミン ソン | Recipient block mold and its uses |
| CN104042436A (en) * | 2013-03-12 | 2014-09-17 | 老史蒂文·A·舒宾 | Systems and methods related to collection of biological fluids |
| CN205719722U (en) * | 2016-06-21 | 2016-11-23 | 武汉赛维尔生物科技有限公司 | A kind of biological tissue paraffin-embedded mould |
| CN205958337U (en) * | 2016-08-05 | 2017-02-15 | 徐菲菲 | Pathological tissue paraffin embedding mould |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108389492A (en) * | 2018-04-27 | 2018-08-10 | 佛山拾念科技有限公司 | A kind of dismountable bioconversion medium plant cell mold |
| CN110146366A (en) * | 2019-06-11 | 2019-08-20 | 河南科技学院 | A kind of mold being used to prepare pathologic tissue packing wax stone |
| CN110146366B (en) * | 2019-06-11 | 2022-04-05 | 河南科技学院 | A mould for preparing wax stone for pathological tissue embedding |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107053543B (en) | 2019-01-04 |
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