CN110146366A - A kind of mold being used to prepare pathologic tissue packing wax stone - Google Patents
A kind of mold being used to prepare pathologic tissue packing wax stone Download PDFInfo
- Publication number
- CN110146366A CN110146366A CN201910500712.2A CN201910500712A CN110146366A CN 110146366 A CN110146366 A CN 110146366A CN 201910500712 A CN201910500712 A CN 201910500712A CN 110146366 A CN110146366 A CN 110146366A
- Authority
- CN
- China
- Prior art keywords
- wax stone
- framed
- mold core
- mandril
- tissue
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
- G01N2001/366—Moulds; Demoulding
Abstract
The present invention relates to a kind of molds for being used to prepare pathologic tissue packing wax stone, mold core including framed and framed inside, several wax stone grooves are equipped in the upper surface of mold core, the pilot hole of vertically communicated mold core is equipped at the center of each wax stone bottom portion of groove, matching and vertical perforative mandril is equipped in each pilot hole, the upper end of every mandril is fixed with horizontal top plate, spring is set in the lower part of every mandril, the casting stretched out straight up is fixed at the center of the upper surface of each top plate, the wax stone morphosis that the present invention prepares is complete and has central hole, tiny tissue or relatively thin tissue can be put into hole when use, it will be embedded in wax liquor filling pore again, hole can limit moving and preventing it from toppling over for tissue, play the role of fixing organization direction, convenient for switching to reason when user's slice Think position, at the same can effectively avoid because with tweezers repeatedly clamping tissue adjust its towards when tissue caused by mechanical injuries.
Description
Technical field
The present invention relates to the technical field of medical instrument, in particular to one kind is used to prepare tiny or relatively thin pathological tissue packet
Bury the mold with wax stone.
Background technique
Histopathologic examination is a kind of inspection method based on microsection technology of preparing, due to body tissue quality pine
It is soft, it is difficult to be efficiently sliced and more difficult obtain meets the ultra-thin section of microexamination, it is therefore desirable to use some supports
The production being sliced again after the embedding medium of effect, such as paraffin filling tissue cellularity, this slice are referred to as " stone
Wax slice ".For paraffin embedding, the method generallyd use be the paraffin of melting is poured into embedding frame in, paraffin it
The preceding tissue block crossed with tweezers clamping through transparent and waxdip step process is put into the paraffin of melting, and adjusts tissue block with tweezers
Direction to be sliced when switch to desired part, behind the direction for adjusting tissue block, allow wax stone condense or be put at room temperature it is cold
Accelerate its solidification in water；Embedding frame is removed after wax stone solidification takes out the subsequent sectioning of wax stone progress.In the process extremely
There are three factors less will affect the quality and efficiency of organization embedding:
First, adjust tissue block embedding towards when need to move repeatedly the position or orientation of tissue block, and paraffin is at room temperature
Solidify quickly, it may appear that do not adjust tissue block towards and wax liquor just solidified the phenomenon that, make slice cannot switch to ideal
Position；Second, allowing to adjust direction before paraffin, since tissue block lacks auxiliary branch in melt paraffin
Support is also easy that positional shift occurs before wax liquor solidifies or topples over, especially some tiny pipe-cavity-like tissue such as Mouse Bones
Bone or lamellar structure such as chicken embryo vitellinae membrana etc.；Third, needing clamping group repeatedly when adjusting tissue block position or orientation with tweezers
It knits, will cause the mechanical injuries of tissue, it is possible to will affect subsequent observation and judgement to lesion tissue.
Summary of the invention
In view of above-mentioned the organization embedding process the problem of, the present invention provides one kind and is used to prepare pathologic tissue packing
With the mold of wax stone.
Particular content is as follows: a kind of mold being used to prepare pathologic tissue packing wax stone, which includes framed and peace
Mold core mounted in framed inside, it is characterized in that:
The framed framework for rectangular shape, the mold core match and are arranged in framed inside, framed with framed
Bottom is fixed with bottom plate, is equipped with one week convex edge stretched out in horizontal direction, the mold core side in the upper edge of framed medial surface
Equipped with vertical guide, be equipped with the vertical and sliding slot with guide rail on framed medial surface, the mold core by guide rail with
Sliding slot matching on framed makes the sliding pair that mold core is vertical in framed internal formation；
The upper surface of the mold core is equipped with several wax stone grooves, is equipped at the center of each wax stone bottom portion of groove vertically communicated
The pilot hole of mold core, each pilot hole is interior to be equipped with matching and vertical perforative mandril, and the lower end of every mandril is fixed on bottom
The upper surface of plate, the upper end of every mandril are fixed with horizontal top plate, the bottom pair of the corresponding wax stone groove of every piece of top plate
It should match, be set with spring in the lower part of every mandril, the upper end of the spring is supported on the lower end branch of the bottom of mold core, spring
Support is in the upper surface of bottom plate, and mold core is supported on the lower surface of top plate by the bottom of wax stone groove, the table on each top plate
The center in face is fixed with the casting stretched out straight up, and the upper surface of mold core is stretched out in the upper end of each casting.
Preferably, the casting is cylindric or straight quadrangular shape.
Preferably, the wax stone groove is inverted positive quadrangular frustum pyramid shaped.
Preferably, the framed bottom plate is fixed by screws in framed bottom surface.
Preferably, the lower end of every mandril passes through screw and fixes with bottom plate.
Preferably, the described framed, bottom plate, mold core, mandril, top plate and casting are all made of stainless steel material or copper material
Matter production.
Advantageous effects of the invention:
The present invention relates to a kind of mold for being used to prepare pathologic tissue packing wax stone, the mold core including framed and framed inside,
It is equipped with several wax stone grooves on the upper surface of mold core, is equipped with vertically communicated mold core at the center of each wax stone bottom portion of groove
Pilot hole is equipped with matching and vertical perforative mandril in each pilot hole, and the upper end of every mandril is fixed with horizontal
Top plate is set with spring in the lower part of every mandril, is fixed at the center of each cover top surface and stretches out straight up
Casting, wax stone morphosis prepared by the present invention is complete and has hole, by tiny tissue or relatively thin group when use
It knits and is put into hole, then wax liquor filling pore is embedded, hole can limit moving and preventing it from toppling over for tissue, play solid
Determine the effect of tissue direction, switch to desired part in slice convenient for user, the presence of central hole can effective control group
Direction when block embedding is knitted, is avoided with tweezers clamping tissue repeatedly, so also avoids tissue damage caused by tweezers and shadow
The observation and judgement of slice lesion are rung, while correspondingly-shaped and size can be selected according to the form and dimension of pathological tissue
The wax stone of the be poured formation of casting makes the shape of hole in wax stone match as far as possible with the shape of pathological tissue.
Detailed description of the invention
Fig. 1 is a kind of schematic perspective view of mold for being used to prepare pathologic tissue packing wax stone；
Fig. 2 is the schematic diagram of the section structure of Fig. 1；
Fig. 3 is the schematic perspective view of mold core；
Fig. 4 is framed schematic perspective view；
Fig. 5 is the wax stone that die casting of the invention is formed (by taking cylindrical casting as an example)；
Fig. 6 is a kind of schematic diagram (pushing mold core, take out wax stone) of embodiment concrete operations of the invention；
In figure: 1. framed, 11. convex edges, 12. sliding slots, 13. framed bottoms, 2. mold cores, 21. wax stone grooves, 22. pilot holes,
23. guide rail, 3. bottom plates, 31. screws, 41. casting, 42. top plates, 43. mandrils, 44. springs, 5. wax stones, 51. central holes.
Specific embodiment
Embodiment one, referring to Fig. 1-6, a kind of mold being used to prepare pathologic tissue packing wax stone, which includes mould
Frame and the mold core being mounted in framed；
The framed framework for rectangular shape, the mold core match and are arranged in framed inside, framed with framed
Bottom is fixed with bottom plate, is equipped with one week convex edge stretched out in horizontal direction, the mold core side in the upper edge of framed medial surface
Equipped with vertical guide rail, be equipped with sliding slot vertical and with guide rail in framed medial surface, the mold core by guide rail with
Framed upper hopper chute matching makes the sliding pair that mold core is vertical in framed internal formation；
The upper surface of the mold core is equipped with several wax stone grooves, is equipped at the center of each wax stone bottom portion of groove vertically communicated
The pilot hole of mold core is equipped with matching and vertical perforative mandril in each pilot hole, and the lower end of every mandril is fixed on
The upper surface of bottom plate, the upper end of every mandril are fixed with horizontal top plate, the bottom of the corresponding wax stone groove of every piece of top plate
Corresponding matching is set with spring in the lower part of every mandril, and the upper end of the spring is supported on the lower end of the bottom of mold core, spring
It is supported on the upper surface of bottom plate, and mold core is supported on the lower surface of top plate by the bottom of wax stone groove, the table on each top plate
The center in face is fixed with the casting stretched out straight up, and the upper surface of mold core is stretched out in the upper end of each casting.
The casting is cylindric or straight quadrangular shape, can be selected according to the form flexible in size of quasi- investing tissue
The straight quadrangular shape casting of the casting of correspondingly-shaped, the i.e. cylindrical casting of different-diameter or different-thickness forms casting
The shape of hole matches with the shape of pathological tissue as far as possible in wax stone, embeds convenient for the orientation of pathological tissue.
The wax stone groove is inverted positive quadrangular frustum pyramid shaped, and the wax stone of the be poured formation of wax stone groove of the shape can
Effectively completely demould.
The bottom plate is fixed by screws in framed bottom surface, disassembly, peace convenient for mold core and its internal other component
Dress and maintenance cleaning.
The lower end of every mandril passes through screw and fixes with bottom plate, according to use demand, can flexibly dismount mandril and top
Plate replaces casting of different shapes in turn.
The described framed, bottom plate, mold core, mandril, top plate and casting are all made of stainless steel material or copper material production.
The mold is mainly used for the embedding of tiny or relatively thin pathological tissue, can be tiny pipe-cavity-like tissue, such as mouse
Femur, Xenopus laevis bone, zebra fish juvenile fish etc., or relatively thin pathological tissue, as sheet chicken embryo vitellinae membrana, chick chorioallantoic membrane,
Mesenterium etc..
Mold use process of the invention and principle:
The casting of the form or size selection correspondingly-shaped organized when according to practical embedding is simultaneously mounted on mold, checks that mold is protected
Card mold core is supported naturally under the effect of spring force and is adjacent on top plate, and the paraffin of melting is then cast in wax stone
In groove, the face of be poured paraffin liquid is not higher than the upper surface of mold core, according to actual needs the quantity of selection casting wax stone groove,
Casting terminate and at room temperature after wax liquor surface layer solidification after, mold is integrally put into cold water accelerate paraffin solidification；It is to be cast
Paraffin solidify completely after, gently press down on mold core with hand, move mold core gradually downward, at the same solidify wax stone relative to
The top plate that wax stone groove is gradually supported by mandril ejects, and realizes demoulding, then gently lifting wax stone upwards with hand makes itself and casting
Between completely disengage to get to the central hole with specific shape, wax stone that structure is intact, finally the wax stone of preparation is put
In on platform, the pipe of quasi- embedding-cavity-like tissue or lamellar structure are put into central hole, with the position of tweezers adjustment pathological tissue
Set and angle, make to intend in pathological tissue the destination region of slice close to wax stone hole bottom, wax is filled into central hole
The wax stone for being embedded with pathological tissue can be adhered on platform wood after wax liquor solidification and be sliced by liquid.
Wax stone morphosis that the present invention prepares is complete and has hole, by tiny tissue or relatively thin group when use
It knits and is put into hole, then wax liquor filling pore is embedded, hole can limit moving and preventing it from toppling over for tissue, play solid
Determine the effect of tissue direction, switch to desired part in slice convenient for user, the presence of central hole can effective control group
Knit direction when block embedding, avoid with tweezers repeatedly clamping tissue and the tissue damage that may cause and influence to slice lesion
Observation and judgement, while the casting pouring wax stone of correspondingly-shaped and size can be selected according to the form and dimension of pathological tissue,
The shape of wax stone hole is set to match as far as possible with the shape of pathological tissue.
Claims (6)
1. a kind of mold for being used to prepare pathologic tissue packing wax stone, which includes mould that is framed and being mounted on framed inside
Core, it is characterized in that:
The framed framework for rectangular shape, the mold core match and are arranged in framed inside, framed with framed
Bottom is fixed with bottom plate, is equipped with one week convex edge stretched out in horizontal direction, the mold core side in the upper edge of framed medial surface
Equipped with vertical guide, be equipped with the vertical and sliding slot with guide rail on framed medial surface, the mold core by guide rail with
Sliding slot matching on framed makes the sliding pair that mold core is vertical in framed internal formation；
The upper surface of the mold core is equipped with several wax stone grooves, is equipped at the center of each wax stone bottom portion of groove vertically communicated
The pilot hole of mold core, each pilot hole is interior to be equipped with matching and vertical perforative mandril, and the lower end of every mandril is fixed on bottom
The upper surface of plate, the upper end of every mandril are fixed with horizontal top plate, the bottom pair of the corresponding wax stone groove of every piece of top plate
It should match, be set with spring in the lower part of every mandril, the upper end of the spring is supported on the lower end branch of the bottom of mold core, spring
Support is in the upper surface of bottom plate, and mold core is supported on the lower surface of top plate by the bottom of wax stone groove, the table on each top plate
The center in face is fixed with the casting stretched out straight up, and the upper surface of mold core is stretched out in the upper end of each casting.
2. the mold according to claim 1 for being used to prepare pathologic tissue packing wax stone, it is characterized in that: the casting
For cylindric or straight quadrangular shape.
3. the mold according to claim 1 for being used to prepare pathologic tissue packing wax stone, it is characterized in that: the wax stone
Groove is inverted positive quadrangular frustum pyramid shaped.
4. the mold according to claim 1 for being used to prepare pathologic tissue packing wax stone, it is characterized in that: described is framed
Bottom plate is fixed by screws in framed bottom surface.
5. the mold according to claim 1 for being used to prepare pathologic tissue packing wax stone, it is characterized in that: every mandril
Lower end passes through screw and fixes with bottom plate.
6. the mold according to claim 1 for being used to prepare pathologic tissue packing wax stone, it is characterized in that: the mould
Frame, bottom plate, mold core, mandril, top plate and casting are all made of stainless steel material or copper material production.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910500712.2A CN110146366B (en) | 2019-06-11 | 2019-06-11 | A mould for preparing wax stone for pathological tissue embedding |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910500712.2A CN110146366B (en) | 2019-06-11 | 2019-06-11 | A mould for preparing wax stone for pathological tissue embedding |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110146366A true CN110146366A (en) | 2019-08-20 |
| CN110146366B CN110146366B (en) | 2022-04-05 |
Family
ID=67591003
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910500712.2A Active CN110146366B (en) | 2019-06-11 | 2019-06-11 | A mould for preparing wax stone for pathological tissue embedding |
Country Status (1)
| Country | Link |
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| CN (1) | CN110146366B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111889621A (en) * | 2020-08-10 | 2020-11-06 | 马鞍山市兴隆铸造有限公司 | Wax block making equipment for investment machining casting process |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5665398A (en) * | 1995-11-20 | 1997-09-09 | Mccormick; James B. | Apparatus for embedding tissue samples |
| CN1920558A (en) * | 2005-12-27 | 2007-02-28 | 胡苹 | Wax module for organizing chip array |
| CN202471473U (en) * | 2011-09-14 | 2012-10-03 | 饶莹 | Device for manufacturing tissue chip at one time |
| CN107053543A (en) * | 2017-04-24 | 2017-08-18 | 华中科技大学 | Suitable for anaerobic resin imbedded mold |
| CN207456912U (en) * | 2017-09-26 | 2018-06-05 | 西安培华学院 | A kind of pathologic tissue packing die |
| CN109443897A (en) * | 2018-12-27 | 2019-03-08 | 沈阳安真医疗器械有限公司 | Multifunctional unit tissue embedding box |
-
2019
- 2019-06-11 CN CN201910500712.2A patent/CN110146366B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5665398A (en) * | 1995-11-20 | 1997-09-09 | Mccormick; James B. | Apparatus for embedding tissue samples |
| CN1920558A (en) * | 2005-12-27 | 2007-02-28 | 胡苹 | Wax module for organizing chip array |
| CN202471473U (en) * | 2011-09-14 | 2012-10-03 | 饶莹 | Device for manufacturing tissue chip at one time |
| CN107053543A (en) * | 2017-04-24 | 2017-08-18 | 华中科技大学 | Suitable for anaerobic resin imbedded mold |
| CN207456912U (en) * | 2017-09-26 | 2018-06-05 | 西安培华学院 | A kind of pathologic tissue packing die |
| CN109443897A (en) * | 2018-12-27 | 2019-03-08 | 沈阳安真医疗器械有限公司 | Multifunctional unit tissue embedding box |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111889621A (en) * | 2020-08-10 | 2020-11-06 | 马鞍山市兴隆铸造有限公司 | Wax block making equipment for investment machining casting process |
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| Publication number | Publication date |
|---|---|
| CN110146366B (en) | 2022-04-05 |
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