CN110343610A - A kind of laboratory liquid installation for fermenting of real-time detection hydrogen sulfide - Google Patents
A kind of laboratory liquid installation for fermenting of real-time detection hydrogen sulfide Download PDFInfo
- Publication number
- CN110343610A CN110343610A CN201910756567.4A CN201910756567A CN110343610A CN 110343610 A CN110343610 A CN 110343610A CN 201910756567 A CN201910756567 A CN 201910756567A CN 110343610 A CN110343610 A CN 110343610A
- Authority
- CN
- China
- Prior art keywords
- real
- tank body
- hydrogen sulfide
- fermenting
- bend pipe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 title claims abstract description 47
- 229910000037 hydrogen sulfide Inorganic materials 0.000 title claims abstract description 47
- 239000007788 liquid Substances 0.000 title claims abstract description 35
- 238000009434 installation Methods 0.000 title claims abstract description 28
- 238000011897 real-time detection Methods 0.000 title claims abstract description 22
- 238000005070 sampling Methods 0.000 claims abstract description 20
- 238000001514 detection method Methods 0.000 claims abstract description 17
- -1 tank body (1) Chemical compound 0.000 claims abstract description 4
- 239000011521 glass Substances 0.000 claims description 13
- 239000000463 material Substances 0.000 claims description 10
- 229920000742 Cotton Polymers 0.000 claims description 6
- 229910001220 stainless steel Inorganic materials 0.000 claims description 6
- 239000010935 stainless steel Substances 0.000 claims description 6
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 6
- 238000000855 fermentation Methods 0.000 abstract description 27
- 230000004151 fermentation Effects 0.000 abstract description 27
- 239000007789 gas Substances 0.000 abstract description 19
- 238000012544 monitoring process Methods 0.000 abstract description 5
- 239000000523 sample Substances 0.000 description 9
- 238000011160 research Methods 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 2
- 238000005189 flocculation Methods 0.000 description 2
- 230000016615 flocculation Effects 0.000 description 2
- 241001411320 Eriogonum inflatum Species 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 150000002898 organic sulfur compounds Chemical class 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/04—Bioreactors or fermenters specially adapted for specific uses for producing gas, e.g. biogas
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
Abstract
The invention discloses a kind of laboratory liquid installation for fermentings of real-time detection hydrogen sulfide, belong to wine brewing engineering field.The present invention solves the problems, such as that laboratory ordinary liquid installation for fermenting is unable to real-time monitoring hydrogen sulfide gas and generates and while drawing fermentation broth sample.A kind of laboratory liquid installation for fermenting of real-time detection hydrogen sulfide, including tank body (1), it is characterised in that: rubber stopper is arranged in tank body (1) tank mouth；Setting sampling syringe needle (2) and bend pipe (5) in the rubber stopper, described sampling syringe needle (2) one end are equipped with seal cap, and the other end is placed in tank body (1)；Described bend pipe (5) one end is placed in tank body (1), and bend pipe (5) middle section is spherical shape, and bend pipe (5) other end passes through rubber tube (9) horizontal connection sulfurated hydrogen detection pipe (10).
Description
Technical field
The invention discloses a kind of laboratory liquid installation for fermentings of real-time detection hydrogen sulfide, belong to wine brewing engineering field.
Background technique
Hydrogen sulfide has rotten egg smell, high volatility, taste threshold low, is that saccharomycete passes through sulfate in fermentation process
Reduction approach forms a kind of gas for being metabolized and generating during required organic sulfur compound.Excessive hydrogen sulfide accumulation will cause fermentation
The flavor defect of wine, therefore the real-time detection of hydrogen sulfide gas is one for needing to carry out in quality yeast breeding in fermentation process
Index.Existing laboratory liquid installation for fermenting can only detect the total amount of hydrogen sulfide gas in fermentation process mostly, can not supervise in real time
Hydrogen sulfide gas production quantity is surveyed, and the absorption of fermented liquid sample would generally interfere the detection of hydrogen sulfide gas in fermentation process
Accuracy.
Summary of the invention
In order to solve the above technical problems, the present invention provides a kind of laboratory liquid state fermentation of real-time detection hydrogen sulfide dresses
It sets, solves laboratory ordinary liquid installation for fermenting and be unable to the generation of real-time monitoring hydrogen sulfide gas and while drawing fermentation broth sample
Problem provides suitably for the generation of hydrogen sulfide gas during laboratory research liquid state fermentation and the variation of fermentation liquid and thallus
Scientific research device.
The technical solution adopted by the present invention to solve the technical problems is:
Rubber stopper is arranged in a kind of laboratory liquid installation for fermenting of real-time detection hydrogen sulfide, including tank body, the tank body tank mouth；Institute
It states setting sampling syringe needle and bend pipe, the first end of the sampling probe in rubber stopper and is equipped with seal cap, the other end is placed in tank body；Institute
The bend pipe one end stated is placed in tank body, and bend pipe middle section is spherical shape, and for all gas generated in fermentation process to be discharged, bend pipe is another
One end passes through rubber tube horizontal connection sulfurated hydrogen detection pipe.
Tank body, rubber stopper, sampling syringe needle and the bend pipe is made of the material for being resistant to high pressure steam sterilization.I.e. material passes through
121 DEG C of 20min do not deform and damaged condition.The installation for fermenting part can be gone out by high steam before scientific experiment
The mode of bacterium directly sterilizes, and guarantees the germ-free condition of installation for fermenting.
The tank body material is glass.In order to monitor concrete condition (blistering, thallus flocculation, hair of fermentation liquid in real time
Zymotic fluid height, fermentation liquid color etc.).
It is 3/5 or less tank body height that the sampling syringe needle, which is placed in the intracorporal length of tank,.
The bend pipe is placed in the intracorporal one end of tank, and length exceeds rubber stopper.
It further include that absorbent cotton is equipped in one end bend pipe for connect sulfurated hydrogen detection pipe.
Sampling syringe needle is stainless steel material.
The absorbent cotton, rubber tube and sulfurated hydrogen detection pipe are assembled under aseptic condition after device high pressure steam sterilization
The present apparatus；Described device needs detection device air-tightness before use.
Beneficial effects of the present invention: a kind of laboratory liquid installation for fermenting of real-time detection hydrogen sulfide provided by the invention,
Fermentation broth sample is drawn in real time by increasing stainless steel syringe needle, prevents gas overflowing, by increasing glass by increasing seal cap
The real-time detections hydrogen sulfide gas production quantity such as bend pipe and sulfurated hydrogen detection pipe, solving laboratory ordinary liquid installation for fermenting cannot be real
When monitoring hydrogen sulfide gas generate and the problem of drawing fermentation broth sample simultaneously, be to vulcanize during laboratory research liquid state fermentation
The generation of hydrogen and the variation of fermentation liquid and thallus provide suitable scientific research device.
Detailed description of the invention
Fig. 1 is a kind of overall schematic of the laboratory liquid installation for fermenting of real-time detection hydrogen sulfide of embodiment；
Fig. 2 is a kind of bottle stopper schematic diagram of the laboratory liquid installation for fermenting of real-time detection hydrogen sulfide of embodiment.
Specific embodiment
Technical problem of the invention for ease of understanding retouches the present invention below in conjunction with drawings and examples in detail
It states, the embodiment is only for explaining the present invention, does not constitute and limits to protection scope of the present invention, all to use equivalent replacement or wait
The technical solution that the mode of effect conversion obtains is fallen within the scope of protection of the present invention.
Embodiment
As shown in Figure 1 to Figure 2, the laboratory liquid installation for fermenting of a kind of real-time detection hydrogen sulfide, including tank body 1, it is described
Rubber stopper is arranged in 1 tank mouth of tank body；Setting sampling syringe needle 2 and bend pipe 5, described 2 one end of sampling syringe needle are equipped in the rubber stopper
Seal cap, the other end are placed in tank body 1；Described 5 one end of bend pipe is placed in tank body 1, and 5 middle section of bend pipe is spherical shape 6, and bend pipe 5 is another
One end passes through 8 horizontal connection sulfurated hydrogen detection pipe 10 of rubber tube.It further include being set in one end bend pipe 5 for connect sulfurated hydrogen detection pipe 9
There is absorbent cotton 7.
Tank body 1, rubber stopper, sampling syringe needle 2 and the bend pipe 5 is made of the material for being resistant to high pressure steam sterilization, tank body 1
Material is glass.It is 3/5 or less tank body height that the sampling syringe needle 2, which is placed in the intracorporal length of tank,.The bend pipe 5 is placed in tank body
One end in 1, length exceed rubber stopper.
A kind of laboratory liquid installation for fermenting of real-time detection hydrogen sulfide of the present embodiment, the rubber stopper of setting can be used for
Ensure the air-tightness of entire installation for fermenting；Stainless steel sampling 2 length of syringe needle of setting need to immerse fermentation liquid level, and (fermentation volume does not surpass
The 3/5 of everfermentation device volume) hereinafter, for drawing fermentation broth sample in real time；It is set to the close of stainless steel sampling probe head-tail 3
Sealing cap 4, when sampling, can remove, and seal when not sampling, prevent gas overflowing；Expose rubber in glass elbow one end of setting
Rubber plug is a little, and the other end is connected in the glass tube of sulfurated hydrogen detection pipe and is required by rubber tube horizontal connection sulfurated hydrogen detection pipe
With one section of absorbent cotton for absorbing water, glass elbow is for being discharged all fermentation gas, to avoid gravity to testing result
Influence, sulfurated hydrogen detection pipe need to keep horizontal, and the central ball of glass elbow is designed for accommodating portion fermentation gas and cold
Solidifying part vapor；Glass tank body, rubber stopper, stainless steel sampling syringe needle and the glass elbow of setting can tolerate high steam and go out
Bacterium, the installation for fermenting can directly be sterilized by way of high pressure steam sterilization before scientific experiment, guarantee the nothing of installation for fermenting
Bacterium state；The glass tank body of setting is convenient for concrete condition (blistering, thallus flocculation, fermentation liquid height, the hair of real time monitoring fermentation liquid
Zymotic fluid color etc.)；The absorbent cotton, rubber tube and sulfurated hydrogen detection pipe are after device high pressure steam sterilization in aseptic condition the following group
Put into the present apparatus；Described device needs detection device air-tightness before use.
The laboratory liquid installation for fermenting of a kind of real-time detection hydrogen sulfide provided in this embodiment, by increasing stainless pin
Head draws fermentation broth sample in real time, prevents gas overflowing, by increasing glass elbow and sulfurated hydrogen detection by increasing seal cap
The real-time detections hydrogen sulfide gas production quantity such as pipe solves laboratory ordinary liquid installation for fermenting and is unable to real-time monitoring hydrogen sulfide gas
The problem of generating and drawing fermentation broth sample simultaneously, generation and hair for hydrogen sulfide gas during laboratory research liquid state fermentation
The variation of zymotic fluid and thallus provides suitable scientific research device.
Claims (6)
1. a kind of laboratory liquid installation for fermenting of real-time detection hydrogen sulfide, including tank body (1), it is characterised in that: the tank body
(1) rubber stopper is arranged in tank mouth；Setting sampling syringe needle (2) and bend pipe (5), described sampling syringe needle (2) one end in the rubber stopper
Equipped with seal cap, the other end is placed in tank body (1)；Described bend pipe (5) one end is placed in tank body (1), and bend pipe (5) middle section is ball
Shape (6), bend pipe (5) other end pass through rubber tube (8) horizontal connection sulfurated hydrogen detection pipe (9).
2. a kind of laboratory liquid installation for fermenting of real-time detection hydrogen sulfide according to claim 1, it is characterised in that: institute
Tank body (1), rubber stopper, sampling syringe needle (2) and the bend pipe (5) stated are made of the material for being resistant to high pressure steam sterilization.
3. a kind of laboratory liquid installation for fermenting of real-time detection hydrogen sulfide according to claim 1, it is characterised in that: institute
Tank body (1) material stated is glass.
4. a kind of laboratory liquid installation for fermenting of real-time detection hydrogen sulfide according to claim 1, it is characterised in that: institute
Stating sampling syringe needle (2) and being placed in the intracorporal length of tank is tank body height 3/5 hereinafter, material is stainless steel.
5. a kind of laboratory liquid installation for fermenting of real-time detection hydrogen sulfide according to claim 1, it is characterised in that: institute
The bend pipe (5) stated is placed in one end in tank body (1), and length exceeds rubber stopper, and material is glass.
6. a kind of laboratory liquid installation for fermenting of real-time detection hydrogen sulfide according to claim 1, it is characterised in that: also
Absorbent cotton (7) are equipped in one end bend pipe (5) including connecting sulfurated hydrogen detection pipe (9).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910756567.4A CN110343610A (en) | 2019-08-16 | 2019-08-16 | A kind of laboratory liquid installation for fermenting of real-time detection hydrogen sulfide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910756567.4A CN110343610A (en) | 2019-08-16 | 2019-08-16 | A kind of laboratory liquid installation for fermenting of real-time detection hydrogen sulfide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110343610A true CN110343610A (en) | 2019-10-18 |
Family
ID=68185239
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910756567.4A Pending CN110343610A (en) | 2019-08-16 | 2019-08-16 | A kind of laboratory liquid installation for fermenting of real-time detection hydrogen sulfide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110343610A (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202063904U (en) * | 2011-05-05 | 2011-12-07 | 四川剑南春（集团）有限责任公司 | White wine fermentation device |
| CN202246675U (en) * | 2011-10-21 | 2012-05-30 | 南京工业大学 | Device for electrolytically promoting anaerobic fermentation |
| CN102798557A (en) * | 2012-08-10 | 2012-11-28 | 中国科学院亚热带农业生态研究所 | Method and reactor for real-time online detection of substance nutrient bio-utilization |
| CN205874361U (en) * | 2016-06-30 | 2017-01-11 | 曲靖师范学院 | Simple and easy laboratory fruit wine fermentation device |
| CN207659453U (en) * | 2017-11-21 | 2018-07-27 | 中国农业科学院草原研究所 | Normal temperature anaerobic fermentation device |
| CN208883864U (en) * | 2018-06-29 | 2019-05-21 | 安徽瑞思威尔科技有限公司 | A kind of small intermittent-type anaerobic ferment devices |
| CN209052695U (en) * | 2018-09-27 | 2019-07-02 | 淮阴师范学院 | A kind of laboratory anaerobic fermentation easy device |
| CN210367700U (en) * | 2019-08-16 | 2020-04-21 | 贵州大学 | Laboratory liquid state fermentation device of real-time detection hydrogen sulfide |
-
2019
- 2019-08-16 CN CN201910756567.4A patent/CN110343610A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202063904U (en) * | 2011-05-05 | 2011-12-07 | 四川剑南春（集团）有限责任公司 | White wine fermentation device |
| CN202246675U (en) * | 2011-10-21 | 2012-05-30 | 南京工业大学 | Device for electrolytically promoting anaerobic fermentation |
| CN102798557A (en) * | 2012-08-10 | 2012-11-28 | 中国科学院亚热带农业生态研究所 | Method and reactor for real-time online detection of substance nutrient bio-utilization |
| CN205874361U (en) * | 2016-06-30 | 2017-01-11 | 曲靖师范学院 | Simple and easy laboratory fruit wine fermentation device |
| CN207659453U (en) * | 2017-11-21 | 2018-07-27 | 中国农业科学院草原研究所 | Normal temperature anaerobic fermentation device |
| CN208883864U (en) * | 2018-06-29 | 2019-05-21 | 安徽瑞思威尔科技有限公司 | A kind of small intermittent-type anaerobic ferment devices |
| CN209052695U (en) * | 2018-09-27 | 2019-07-02 | 淮阴师范学院 | A kind of laboratory anaerobic fermentation easy device |
| CN210367700U (en) * | 2019-08-16 | 2020-04-21 | 贵州大学 | Laboratory liquid state fermentation device of real-time detection hydrogen sulfide |
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