CN110343151B - Application of verticillium dahliae effector protein VdSCP113 - Google Patents
Application of verticillium dahliae effector protein VdSCP113 Download PDFInfo
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- CN110343151B CN110343151B CN201910646907.8A CN201910646907A CN110343151B CN 110343151 B CN110343151 B CN 110343151B CN 201910646907 A CN201910646907 A CN 201910646907A CN 110343151 B CN110343151 B CN 110343151B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
- A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
- A01N47/44—Guanidine; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
Abstract
The disclosure relates to an application of verticillium dahliae effector protein VdSCP113, which comprises the following steps: the effector protein VDPSC 113 is used for improving plant resistance and/or inducing plant defense response. According to the application, the effector protein VDPSC 113 is used for improving the plant resistance and inducing the plant defense reaction, the efficiency of inducing immune reactions such as ROS outbreak, callose deposition and the like is high, and the plant resistance improvement and the defense reaction induction effect are obvious. The method provides a new way for improving the plant resistance and inducing the plant defense reaction, and has wide application prospect in agricultural production.
Description
Technical Field
The disclosure relates to the technical field of microbial protein application, in particular to application of an effector protein VdSCP113 in improving plant resistance and inducing plant defense response.
Background
Pathogen-associated molecular patterns (PAMPs) refer to certain highly conserved molecular structures shared by the surfaces of pathogenic microorganisms,are generally characteristic of pathogenic microorganisms, and host cells are not produced, which is essential for the survival or pathogenicity of the microorganism. Plants recognize PAMPs molecules via Pattern Recognition Receptors (PRRs) on the cell membrane, thereby activating pathogen-associated molecular patterns of plants to trigger immunity (PTI) against infestation by pathogenic microorganisms. After the PAMPs molecules are recognized by plants, a series of defense responses, such as intracytoplasmic Ca, can be generated2+Changes in concentration, activation of MAPKs kinase signaling pathways, ROS outbreak, cytosolic acidification, ethylene synthesis, expression of disease-course related genes, cell wall callose precipitation, phytoalexin synthesis, cellular ion leakage and production of HR responses, etc.
The ROS outbreak plays an important role in the plant disease-resistant defense response induced by PAMPs molecules, participates in regulating stomatal movement, promotes cell apoptosis, directly or indirectly kills invading pathogenic bacteria, can be used as a second messenger to activate and regulate the expression of various defense-related genes in the plant body at the transcription level, participates in the establishment of system acquired resistance and the like. The cell wall callose precipitates, and can strengthen the cell wall of the plant to prevent the invasion of pathogenic microorganisms and the colonization of most pathogenic microorganisms, thereby achieving the aim of resisting and defending the pathogenic microorganisms.
However, the induction efficiency of immune responses such as ROS outbreaks, callose deposits and the like is still low at present.
Disclosure of Invention
The purpose of the present disclosure is to improve the efficiency of induction of substances that induce immune responses such as ROS outbreak, callose deposition, and the like.
In order to achieve the above object, the present disclosure provides a use of verticillium dahliae effector protein VdSCP113, wherein an amino acid sequence of the effector protein VdSCP113 is shown in SEQ ID NO: 2, the application comprises the following steps: the effector protein VDPSC 113 is used for improving plant resistance and/or inducing plant defense response.
Optionally, the increasing plant resistance comprises:
improving the resistance of the plant to at least one pathogen of botrytis cinerea, phytophthora capsici, verticillium dahliae, fusarium and phyllotreta fungi;
the induction of a plant defense response comprising:
inducing and reducing the harm of at least one pathogen of botrytis cinerea, phytophthora capsici, verticillium dahliae, fusarium and phyllotreta to plants.
Optionally, the plant comprises at least one of tobacco, cotton, pepper, arabidopsis, potato, and tomato.
Optionally, the application concentration of the effector protein VdSCP113 is 0.001-10 mu M.
The present disclosure also provides a method of increasing resistance and/or inducing a plant defense response in a plant, the method comprising:
introducing a coding gene of an effector protein VdSCP113 into a target plant to obtain a plant with improved resistance and/or a defense reaction; alternatively, the first and second electrodes may be,
applying the effector protein VDPSC 113 to a target plant to obtain a plant with improved resistance and/or a defense reaction;
wherein, the polynucleotide sequence of the coding gene of the effector protein VdSCP113 is shown in SEQ ID NO: 1 is shown in the specification; the amino acid sequence of the effector protein VDPSC 113 is shown as SEQ ID NO: 2, respectively.
Alternatively, the gene encoding the effector protein VdSCP113 is introduced into a target plant through a plant expression vector.
Optionally, the plant expression vector comprises a Ti-based plasmid vector and/or a viral vector.
Optionally, the plant comprises at least one of tobacco, cotton, pepper, arabidopsis, potato, and tomato.
The present disclosure also provides the use of an effector protein VdSCP113 in the preparation of a pesticide for increasing plant resistance and/or inducing a plant defence response, the effector protein VdSCP113 amino acid sequence being as set forth in SEQ ID NO: 2, respectively.
Optionally, the plant comprises at least one of tobacco, cotton, pepper, arabidopsis, potato, and tomato.
By adopting the technical scheme, when the effector protein VdSCP113 is used for improving the plant resistance and inducing the plant defense reaction, the efficiency of inducing immune reactions such as ROS outbreak, callose deposition and the like is higher, and the plant resistance improvement and the defense reaction induction effect are obvious. The method provides a new way for improving the plant resistance and inducing the plant defense reaction, and has wide application prospect in agricultural production.
Additional features and advantages of the disclosure will be set forth in the detailed description which follows.
Drawings
The accompanying drawings, which are included to provide a further understanding of the disclosure and are incorporated in and constitute a part of this specification, illustrate embodiments of the disclosure and together with the description serve to explain the disclosure without limiting the disclosure. In the drawings:
FIG. 1 is a graph showing the results of the experiment in which the response protein VdSCP113 induces ROS reaction in tobacco leaves in example 1 of the present disclosure.
FIG. 2 is a graph showing the results of experiments in which the responsive protein VdSCP113 induces the accumulation of callose in tobacco leaves in example 1 of the present disclosure.
FIG. 3 is a graph showing the results of experiments on the increase of expression level of genes related to tobacco defense response by the effector protein VdSCP113 in example 1 of the present disclosure.
FIG. 4 is a graph showing the results of experiments in which the response protein VdSCP113 in example 2 of the present disclosure induces resistance response of tobacco to Botrytis cinerea.
Detailed Description
The following describes in detail specific embodiments of the present disclosure. It should be understood that the detailed description and specific examples, while indicating the present disclosure, are given by way of illustration and explanation only, not limitation.
The first aspect of the disclosure provides a use of verticillium dahliae effector protein VdSCP113, wherein the amino acid sequence of the effector protein VdSCP113 is shown as SEQ ID NO: 2, the application comprises the following steps: the effector protein VDPSC 113 is used for improving plant resistance and/or inducing plant defense response.
The research of the inventor of the present disclosure finds that after the coding gene of VdSCP113 is knocked out from Verticillium dahliae, the pathogenic phenotype of the mutant strain is identified, and the identification result shows that the Verticillium dahliae mutant strain with the VdSCP113 coding gene knocked out has no significant change in the pathogenicity of cotton and tobacco, which indicates that VdSCP113 is not a virulence factor of Verticillium dahliae; after injecting verticillium dahliae effector protein VDPSC 113 into plant leaves, such as tobacco leaves, the generation of ROS in the leaves and the accumulation of callose can be detected, and the expression level of some defense response related genes of the injected plants is also obviously up-regulated, such as HR response marker genes HSR203, H1N1 and GRAS 2; disease course related genes PR1, PR4 and Glnb. Thus, the effector protein VdSCP113 may be used to increase plant resistance and/or induce a plant defence response.
In the technical scheme, when the effector protein VdSCP113 is used for improving the plant resistance and inducing the plant defense reaction, the efficiency of inducing immune reactions such as ROS outbreak, callose deposition and the like is higher, and the plant resistance improvement and the defense reaction induction effect are obvious. The method provides a new way for improving the plant resistance and inducing the plant defense reaction, and has wide application prospect in agricultural production.
According to the present disclosure, increasing plant resistance includes increasing the resistance of the target plant to pathogen infection, which can reduce the infection degree of the target plant by the pathogen and limit the further spread of the pathogen in the target plant. The pathogens referred to in the present disclosure include viruses, bacteria, oomycetes, fungi, nematodes and the like, and the types of pathogens may be selected from a wide range. Preferably, the effector protein VDPSCP 113 is capable of increasing the resistance of a plant to at least one pathogen of Botrytis cinerea, Phytophthora capsici, Verticillium dahliae, Fusarium sp.
In accordance with the present disclosure, inducing a plant defense response includes inducing the target plant to activate its own defense system to reduce the risk of infestation of the target plant by pathogens, the type of which can be selected within a wide range. Preferably, the effector protein VDPSCP 113 is capable of inducing and reducing the damage of at least one pathogen of botrytis cinerea, phytophthora capsici, verticillium dahliae, fusarium and phyllotreta to plants.
The variety of the plant may be selected within a wide range according to the present disclosure. Preferably, the plant may include at least one of tobacco, cotton, pepper, arabidopsis, potato and tomato. The effector protein VDPSC 113 has improved resistance and better effect of inducing defense response to plants in the above-mentioned preferred range.
According to the present disclosure, the applied concentration of the effector protein VdSCP113 may be selected within a wide range. The application concentration of the effector protein VDPSCP 113 of the present disclosure can be 0.001-10 mu M, and preferably 0.01-1 mu M. Within the preferred concentration range, the effector protein VDPSC 113 can improve plant resistance and/or induce plant defense response more rapidly and better.
A second aspect of the present disclosure provides a method of increasing resistance and/or inducing a plant defence response, the method comprising:
introducing a coding gene of an effector protein VdSCP113 into a target plant to obtain a plant with improved resistance and/or a defense reaction; alternatively, the first and second electrodes may be,
applying the effector protein VDPSC 113 to a target plant to obtain a plant with improved resistance and/or a defense reaction;
wherein, the polynucleotide sequence of the coding gene of the effector protein VdSCP113 is shown in SEQ ID NO: 1 is shown in the specification; the amino acid sequence of the effector protein VDPSC 113 is shown as SEQ ID NO: 2, respectively.
Introducing a coding gene of an effector protein VdSCP113 into a target plant, transiently transfecting the target plant with the coding gene of the effector protein VdSCP113, and expressing to generate the effector protein VdSCP 113; or, the effector protein VdSCP113 or the composition containing the effector protein VdSCP113 is directly introduced into a target plant, and after the plant recognizes the effector protein VdSCP113, a series of defense reactions are generated, such as ROS generation, callose accumulation, expression level up-regulation of genes related to the defense reactions and the like, so that the purposes of improving plant resistance and/or inducing plant defense reactions are achieved. The process of the method of the present disclosure is relatively simple and easy to control.
According to the present disclosure, the gene encoding the effector protein VdSCP113 may preferably be introduced into a plant of interest via a plant expression vector. The coding gene is introduced by the plant expression vector, the method is simple and rapid, and the success rate is relatively high.
The type of plant expression vector may be selected within a wide range in accordance with the present disclosure. Preferably, the plant expression vector may comprise a Ti-based plasmid vector and/or a viral vector. The vector is easy to obtain and low in cost, and the vector is easy to carry the coding gene, so that the success rate of introducing the coding gene into and transfecting a target plant is higher.
The variety of the plant may be selected within a wide range according to the present disclosure. Preferably, the plant may include at least one of tobacco, cotton, pepper, arabidopsis, potato and tomato. The disclosed method has improved resistance and better defense response induction effects on the plants.
A third aspect of the present disclosure provides the use of an effector protein VdSCP113 in the preparation of a pesticide for increasing plant resistance and/or inducing a plant defence response, the effector protein VdSCP113 amino acid sequence being as set forth in SEQ ID NO: 2, respectively.
The effector protein VDPSCP 113 can be formulated into a pesticide with other acceptable adjuvants and the pesticide can be used to increase plant resistance and/or induce a plant defense response. The method provides a new raw pesticide selection for preparing the pesticide for improving the plant resistance and inducing the plant defense reaction, and has wide application prospect in pesticide production.
The variety of the plant may be selected within a wide range according to the present disclosure. Preferably, the plant may include at least one of tobacco, cotton, pepper, arabidopsis, potato and tomato. The pesticide containing the effector protein VDPSCP 113 disclosed by the invention has better effects on improving the resistance of the plants and inducing defense response.
The present disclosure is further illustrated by the following examples, but is not to be construed as being limited thereby.
The amino acid sequence of the effector protein VdSCP113 related to the disclosed embodiment is shown as SEQ ID NO: 2, respectively. The preparation method of the effector protein VDPSC 113 comprises the following steps: constructing a recombinant expression vector, performing prokaryotic expression by using escherichia coli, carrying out Maltose Binding Protein (MBP) labeling in the expression vector, and purifying by using an MBP TraPP prepacked column to obtain the target protein.
Example 1 Effector protein VDPSC 113 elicits tobacco immune responses
(1) Effector VDPSC 113 induces ROS reaction in tobacco leaves
About 20. mu.L of 100nM effector protein VDPSCP 113 was injected into 3-4 weeks old tobacco leaves from the back of the leaves using a 1mL syringe without a needle. Tris-HCl (20nM, pH8.0) at the same concentration was used as a negative control. After 6h of injection, the treated tobacco leaves are washed clean with deionized water, placed in DAB dye liquor, incubated for 8h at 25 ℃ in the dark, the dye liquor is removed, the dyed tobacco leaves are washed clean with deionized water, decolorized with 95% ethanol, observed with a stereomicroscope and photographed.
As a result: tobacco leaf injected with VDPSCP 113 effector protein, the injection region has a large amount of H2O2(ii) accumulation of (d); tobacco leaf injected with Tris-HCl, injection zone H2O2The accumulation of (b) is extremely weak, as shown in fig. 1.
The above results demonstrate that the effector protein VdSCP113 can induce the ROS reaction in tobacco leaves.
(2) Effect protein VdSCP113 induces callose accumulation of tobacco leaf
About 20. mu.L of 100nM effector protein VDPSCP 113 was injected into 3-4 weeks old tobacco leaves from the back of the leaves using a 1mL syringe without a needle. Tris-HCl (20nM, pH8.0) at the same concentration was used as a negative control. After 2 days of injection, the leaves of the injection area were washed with deionized water, then decolorized with 95% ethanol, the remaining ethanol was washed with deionized water, the leaves were placed in 150mM phosphate buffer containing 0.1% (W/V) aniline blue at pH9.5, dark treated for 2h, and then the leaves were taken out and observed with a fluorescence microscope and photographed.
As a result: tobacco leaf blades injected with the effector protein VDPSCP 113 have a large number of blue spots in the injection area; in tobacco leaves injected with Tris-HCl, the injection zone had only a few blue spots, as shown in FIG. 2.
The above results indicate that the effector protein VdSCP113 can induce callose accumulation in tobacco leaves.
(3) Effector VDPSC 113 for improving expression level of genes related to tobacco defense response
Approximately 20. mu.L of 100nM effector protein VDPSCP 113 was injected into 4-week-old tobacco leaves from the back of the leaves using a 1mL syringe without a needle. Tris-HCl (20nM, pH8.0) at the same concentration was used as a negative control. After 12h of injection, the leaves in the injection area are collected, RNA is extracted and is reversely transcribed into cDNA, and the expression level of the defense reaction related genes is detected by RT-PCR.
As a result: tobacco lamina injected with effector protein VDPSCP 113, HR response marker genes HSR203, H1N1 and GRAS 2; the pathogenesis-related genes PR1, PR4 and Glnb are induced to express to a certain degree. The results of the experiment are shown in FIG. 3.
The results show that the effector protein VDPSCP 113 can effectively improve the expression level of genes related to tobacco defense.
Example 2 the Effector protein VDPSC 113 induces resistance reactions of tobacco to pathogenic bacteria
Approximately 20. mu.L of 100nM effector protein VDPSCP 113 was injected into 4-week-old tobacco leaves from the back of the leaves using a 1mL syringe without a needle. Tris-HCl (20nM, pH8.0) at the same concentration was used as a negative control. After 12-24 h injection, puncture treatment is carried out on the injection area by using a needle, and 5 muL, 5X 10 prepared in advance is used6spores/mL of different pathogenic bacteria spore suspensions to be detected are dripped right above the needle eye, cultured under the conditions of 25 ℃ and 80% humidity, and the area of each lesion is observed and measured after inoculation for 3-5 d. The results of the experiment are shown in FIG. 4. The inhibition of the effector protein VDPSC 113 on tobacco leaf lesions caused by various pathogenic bacteria is shown in Table 1.
TABLE 1 inhibition of the Effect protein VDPSC 113 against lesions of tobacco leaves caused by various pathogenic bacteria
The results show that the effector protein VDPSCP 113 can obviously induce the resistance reaction of tobacco to botrytis cinerea, phytophthora capsici, verticillium dahliae, fusarium and phyllomycetes.
Example 3 Induction of the resistance response of different plants to Botrytis cinerea by the Effector protein VDPSC 113
The method comprises the steps of spraying or root-irrigating an effector protein VdSCP113 solution with the concentration of about 100nM to treat plants in different experimental groups, spraying 1-2 mL or 10-15 mL of the protein solution to the leaf of each experimental group, and controlling the number of the plants for experiments to be 20. Tris-HCl (20nM, pH8.0) at the same concentration was used as a negative control. After about 24 hours of treatment, the roots were irrigated and inoculated with 20mL of 5X 10 previously prepared6The botrytis cinerea spore suspension of spores/mL is cultured under the conditions of 25 ℃ and 80% humidity, and inoculated for 30d to observe and count the disease condition of plants in each experimental group.
The disease level is divided into 0 level, 1 level, 2 level, 3 level and 4 level according to the disease condition, and the disease conditions of different disease levels are as follows:
level 0: the plant leaves have no disease or almost no disease;
level 1: symptoms appear in 25% of leaves;
and 2, stage: symptoms appear on 26-50% of leaves;
and 3, level: symptoms appear on 51-75% of leaves;
4, level: symptoms appear in 76-100% of leaves.
The proportion (%) of the different disease levels of the plants in the different experimental groups is shown in Table 2.
TABLE 2 proportion of different disease levels (%)
The results show that the effector protein VDPSCP 113 can obviously induce the resistance reaction of pepper, cotton, tomato, potato and Arabidopsis to Botrytis cinerea.
The preferred embodiments of the present disclosure are described in detail with reference to the accompanying drawings, however, the present disclosure is not limited to the specific details of the above embodiments, and various simple modifications may be made to the technical solution of the present disclosure within the technical idea of the present disclosure, and these simple modifications all belong to the protection scope of the present disclosure.
It should be noted that, in the foregoing embodiments, various features described in the above embodiments may be combined in any suitable manner, and in order to avoid unnecessary repetition, various combinations that are possible in the present disclosure are not described again.
In addition, any combination of various embodiments of the present disclosure may be made, and the same should be considered as the disclosure of the present disclosure, as long as it does not depart from the spirit of the present disclosure.
Sequence listing
<110> institute for agricultural product processing of Chinese academy of agricultural sciences
<120> application of verticillium dahliae effector protein VDPSCP 113
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Claims (9)
1. The application of Verticillium dahliae effector protein VdSCP113 is disclosed, wherein the amino acid sequence of the effector protein VdSCP113 is shown in SEQ ID NO: 2, characterized in that the use comprises: the effector protein VDPSC 113 is used for improving the plant resistance and/or inducing the plant defense response;
the method for improving the plant resistance comprises the following steps: improving the resistance of the plant to at least one pathogen of botrytis cinerea, phytophthora capsici, verticillium dahliae, fusarium and phyllotreta fungi;
the induction of a plant defense response comprising: inducing and reducing the harm of at least one pathogen of botrytis cinerea, phytophthora capsici, verticillium dahliae, fusarium and phyllotreta to plants.
2. The use of verticillium dahliae effector protein VdSCP113 according to claim 1, wherein the plant comprises at least one of tobacco, cotton, pepper, arabidopsis, potato and tomato.
3. The use of Verticillium dahliae effector protein VdSCP113 according to claim 1, wherein the concentration of said effector protein VdSCP113 is 0.001-10 μ M.
4. A method of increasing resistance and/or inducing a defence response in a plant, the method including:
introducing a coding gene of an effector protein VdSCP113 into a target plant to obtain a plant with improved resistance and/or a defense reaction; alternatively, the first and second electrodes may be,
applying the effector protein VDPSC 113 to a target plant to obtain a plant with improved resistance and/or a defense reaction;
the method for improving the plant resistance comprises the following steps: improving the resistance of the plant to at least one pathogen of botrytis cinerea, phytophthora capsici, verticillium dahliae, fusarium and phyllotreta fungi;
the induction of a plant defense response comprising: inducing and reducing the harm of at least one pathogen in botrytis cinerea, phytophthora capsici, verticillium dahliae, fusarium and phyllomycetes to plants;
wherein, the polynucleotide sequence of the coding gene of the effector protein VdSCP113 is shown in SEQ ID NO: 1 is shown in the specification; the amino acid sequence of the effector protein VDPSC 113 is shown as SEQ ID NO: 2, respectively.
5. A method according to claim 4, wherein the gene encoding the effector protein VdSCP113 is introduced into the plant of interest via a plant expression vector.
6. The method of claim 5, wherein the plant expression vector comprises a Ti-based plasmid vector and/or a viral vector.
7. The method of any one of claims 4 to 6, wherein the plant comprises at least one of tobacco, cotton, pepper, Arabidopsis, potato and tomato.
8. Use of an effector protein VdSCP113 in the preparation of a pesticide for increasing plant resistance and/or inducing a plant defence response, the effector protein VdSCP113 amino acid sequence being as set forth in SEQ ID NO: 2 is shown in the specification;
the method for improving the plant resistance comprises the following steps: improving the resistance of the plant to at least one pathogen of botrytis cinerea, phytophthora capsici, verticillium dahliae, fusarium and phyllotreta fungi;
the induction of a plant defense response comprising: inducing and reducing the harm of at least one pathogen of botrytis cinerea, phytophthora capsici, verticillium dahliae, fusarium and phyllotreta to plants.
9. The use of the effector protein VdSCP113 according to claim 8 for the preparation of a pesticide, wherein said plant comprises at least one of tobacco, cotton, pepper, Arabidopsis, potato and tomato.
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