CN115413678A - Wettable powder containing verticillium dahliae activator protein and application - Google Patents
Wettable powder containing verticillium dahliae activator protein and application Download PDFInfo
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- CN115413678A CN115413678A CN202211145286.3A CN202211145286A CN115413678A CN 115413678 A CN115413678 A CN 115413678A CN 202211145286 A CN202211145286 A CN 202211145286A CN 115413678 A CN115413678 A CN 115413678A
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- wettable powder
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/12—Powders or granules
- A01N25/14—Powders or granules wettable
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
Abstract
The invention discloses wettable powder containing verticillium dahliae activator protein and application. Relates to the technical field of biological pesticides. The composite material comprises the following raw materials in parts by mass: 5-20 parts of verticillium dahliae activated protein raw powder, 5-10 parts of a wetting agent, 2-6 parts of a dispersing agent, 5-15 parts of a stabilizing agent and the balance of a filler. The wettable powder overcomes the defects that the verticillium dahliae activator protein is easy to agglomerate, has poor water solubility and the like during use, and is proved by tests to have improved solubility, stable property for long-term storage and difficult agglomeration. When the seed dressing agent is used together with a conventional seed dressing agent for seed treatment, the immune disease resistance and stress resistance of wheat can be improved, the seed dressing agent has good control effects on diseases such as wheat sharp eyespot, stalk rot and the like, and the yield increasing effect is obvious.
Description
Technical Field
The invention relates to the technical field of biological pesticides, in particular to wettable powder containing verticillium dahliae activator protein and application thereof.
Background
The pesticide plays an important role in guaranteeing the grain safety in modern agriculture. The pesticide can protect crops and reduce the harm of plant diseases and insect pests, but a large amount of application causes huge pressure on the ecological environment, and adverse effects on the quality safety of agricultural products can be caused due to the overproof pesticide residues: for example, traditional chemical pesticides can cause acute, chronic and "three-cause" harm to humans; pesticide residue in non-target animals and plants will cause changes in animal and plant habits and life rules; pesticides also cause damage to air, water, soil and biodiversity after entering the environment.
Compared with the traditional chemical pesticide, the biological pesticide has the advantages of strong selectivity, difficult generation of drug resistance and small pollution, is mostly low-toxic or slightly-toxic and is safe to people and livestock; the fertilizer is easy to decompose and not easy to pollute agricultural products, and is more beneficial to the quality safety of the agricultural products; safe to natural enemies, does not damage bees, birds, fishes and frogs and has little environmental pollution. Based on the advantages, the biological pesticide is an important way for protecting the ecological environment and guaranteeing the quality and safety of agricultural products.
In recent years, various types of protein elicitors are continuously discovered, molecular targets and molecular mechanisms of the action of the elicitors are continuously and deeply researched, and the elicitors mainly focus on elicitor receptors and signal paths for inducing immune response.
The Verticillium dahliae activator protein VdAL is derived from Verticillium dahliae (Verticillium dahliae) of Verticillium dahliae, belongs to an Aspf 2-like protein and has no protease activity. The protein can improve the verticillium wilt resistance of plants, reduce the influence of verticillium wilt on cotton yield, improve the vitamin C, soluble sugar and/or protein, palatability and the like of plant products, promote the germination of seeds, the growth of plants and the growth of fruits of various plants, improve the fruit setting number, and delay the senescence of the plants. VDAL has development potential in the aspect of biostimulant, and can enhance the disease resistance of rice and promote the growth of rice.
Proteins are basic organic substances constituting cells as the basis of living materials and are the main contributors to life activities, but many proteins are easily modified or inactivated by various factors such as temperature, PH, metal ions, salt concentration, and light, and lose their functions. The wettable powder is a pesticide preparation with a certain fineness prepared by mixing and crushing original pesticide, an auxiliary agent and a filler, can be quickly wetted by water and dispersed into a uniform suspension in use due to the addition of the auxiliary agents such as a wetting agent, a dispersing agent and the like, is convenient to use, and is expected to account for relatively stable share in the pesticide preparation in a long period of time.
Therefore, the problem to be solved by those skilled in the art is how to provide a wettable powder containing verticillium dahliae activator protein and the application thereof.
Disclosure of Invention
In view of this, the invention provides a wettable powder containing verticillium dahliae activator protein and application thereof. By adding a certain amount of additives and fillers, the wettability, the dispersibility and the stability of the product are improved, the use is convenient, the control effect is improved, and the application scenes of conventional spraying, flying control and the like can be met.
In order to achieve the purpose, the invention adopts the following technical scheme:
the wettable powder containing the verticillium dahliae activator protein comprises the following raw materials in 100 parts by mass: 5-20 parts of verticillium dahliae activated protein raw powder, 5-10 parts of a wetting agent, 2-6 parts of a dispersing agent, 5-15 parts of a stabilizing agent and the balance of a filler.
Furthermore, the active ingredient of the verticillium dahliae activated protein raw powder is protein consisting of 260 to 297 amino acids, the molecular weight is about 29 to 33KDa, the content is 2 to 20 percent,
a crude powder of the activated protein of Verticillium dahliae is available from Zhongjie Sifang Biotech, inc. (Inclusion Corp.).
Preferably: wetting agent: one or two of sodium dodecyl benzene sulfonate and sodium dibutyl naphthalene sulfonate.
Preferably: the dispersant is one or a mixture of alkyl naphthalene sulfonate, dialkyl naphthalene sulfonate formaldehyde condensate and lignosulfonate.
Preferably: a stabilizer: one or more of sodium dihydrogen phosphate/potassium dihydrogen phosphate, disodium hydrogen phosphate/potassium hydrogen phosphate, disodium ethylene diamine tetraacetic acid and ascorbic acid.
Preferably: the filler is one or a mixture of a plurality of light calcium carbonate, bentonite, white carbon black, anhydrous sodium sulphate and ammonium salt.
The invention also provides a preparation method of the wettable powder containing the verticillium dahliae activator protein, which comprises the steps of uniformly mixing any one of the raw materials, and airflow-pulverizing to the fineness of 300-500 meshes to obtain the wettable powder containing the verticillium dahliae activator protein.
Furthermore, the content of the active ingredient, namely the verticillium dahliae activator protein is 0.2-1%.
The invention also provides application of any one of the wettable powder in agricultural production.
Preferably, the following components: it can be used for wheat to resist diseases and increase yield.
Preferably: the diseases comprise: wheat stem rot and wheat sheath blight.
According to the technical scheme, compared with the prior art, the invention discloses and provides the wettable powder containing the verticillium dahliae activator protein and the application thereof, and the technical effects are that the defects that the verticillium dahliae activator protein is easy to agglomerate, the water solubility is poor during use and the like are overcome. When the seed dressing agent is used together with a conventional seed dressing agent for seed treatment, the immune disease resistance and stress resistance of wheat can be improved, the prevention and treatment effects on diseases such as wheat sharp eyespot, stem rot and the like are good, and the yield increase effect is obvious.
Detailed Description
The following will clearly and completely describe the technical solutions in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The embodiment of the invention discloses wettable powder containing verticillium dahliae activator protein and application thereof.
Example 1
A wettable powder containing verticillium dahliae activator protein comprises the following raw materials: 5 parts of verticillium dahliae activated protein raw powder, 5 parts of a wetting agent, 2 parts of a dispersing agent, 5 parts of a stabilizing agent and the balance of a filler.
In order to further optimize the technical scheme: wetting agent: one or two of sodium dodecyl benzene sulfonate and sodium dibutyl naphthalene sulfonate.
In order to further optimize the technical scheme: the dispersant is one or a mixture of alkyl naphthalene sulfonate, dialkyl naphthalene sulfonate formaldehyde condensate and lignosulfonate.
In order to further optimize the technical scheme: a stabilizer: one or more of sodium dihydrogen phosphate/potassium dihydrogen phosphate, disodium hydrogen phosphate/potassium hydrogen phosphate, disodium ethylene diamine tetraacetic acid and ascorbic acid.
The preparation method comprises the following steps: the raw materials are evenly mixed and then are pulverized by airflow to 300 meshes of fineness to obtain the product.
In order to further optimize the technical scheme: the filler is one or a mixture of light calcium carbonate, bentonite, white carbon black, anhydrous sodium sulphate and ammonium salt.
Example 2
A wettable powder containing verticillium dahliae activator protein comprises the following raw materials: 15 parts of verticillium dahliae activated protein raw powder, 8 parts of a wetting agent, 5 parts of a dispersing agent, 10 parts of a stabilizing agent and the balance of a filler.
In order to further optimize the technical scheme: wetting agent: one or two of sodium dodecyl benzene sulfonate and sodium dibutyl naphthalene sulfonate.
In order to further optimize the technical scheme: the dispersant is one or a mixture of alkyl naphthalene sulfonate, dialkyl naphthalene sulfonate formaldehyde condensate and lignosulfonate.
In order to further optimize the technical scheme: a stabilizer: one or more of sodium dihydrogen phosphate/potassium dihydrogen phosphate, disodium hydrogen phosphate/potassium dihydrogen phosphate, disodium ethylene diamine tetraacetate and ascorbic acid.
The preparation method comprises the following steps: the raw materials are evenly mixed and crushed into 400 meshes by air flow to obtain the material.
Example 3
A wettable powder containing verticillium dahliae activator protein comprises the following raw materials: 20 parts of verticillium dahliae activated protein raw powder, 10 parts of a wetting agent, 6 parts of a dispersing agent, 15 parts of a stabilizing agent and the balance of a filler.
In order to further optimize the technical scheme: wetting agent: one or two of sodium dodecyl benzene sulfonate and sodium dibutyl naphthalene sulfonate.
In order to further optimize the technical scheme: the dispersant is one or a mixture of alkyl naphthalene sulfonate, dialkyl naphthalene sulfonate formaldehyde condensate and lignosulfonate.
In order to further optimize the technical scheme: a stabilizer: one or more of sodium dihydrogen phosphate/potassium dihydrogen phosphate, disodium hydrogen phosphate/potassium dihydrogen phosphate, disodium ethylene diamine tetraacetate and ascorbic acid.
The preparation method comprises the following steps: the raw materials are evenly mixed and then are pulverized by air flow to 500 meshes of fineness to obtain the product.
Example 4
A wettable powder containing verticillium dahliae activator protein comprises the following raw materials: 20 parts of 2% verticillium dahliae activated protein raw powder, 6 parts of sodium lignosulphonate, 6 parts of sodium dodecyl benzene sulfonate, 3 parts of sodium dihydrogen phosphate, 10 parts of disodium hydrogen phosphate, 1 part of disodium ethylene diamine tetraacetate, 1 part of ascorbic acid, 3 parts of white carbon black and 50 parts of anhydrous sodium sulphate.
The preparation method comprises the following steps: the raw materials are evenly mixed and then are pulverized by airflow to 400 meshes of fineness to obtain the product.
The indexes of 0.4% of verticillium dahliae activator protein wettable powder are detected according to a GB/T5451-2001 pesticide wettable powder wetting time determination method and a GB/T14825-2006 pesticide suspension rate determination method, wherein the wetting time is 30 seconds, the suspension rate is 95%, and the fineness (48 micron sieve) transmittance is 98%.
Example 5
In order to examine the disease-resistant and yield-increasing effects of (0.4%) verticillium dahliae activated protein wettable powder on wheat, treatment modes such as seed dressing, leaf surface spraying and the like are respectively adopted, and meanwhile, a conventional chemical agent is adopted as a contrast.
a. Test site and treatment: a field test is carried out on 50 mu wheat field in a certain family farm in Liuying town in Handan City of Hebei province, and 5 treatment zones, 4 medicament treatment zones and 1 blank control zone are arranged (Table 1). The area of each medicament treatment area is 12 mu, each treatment area is provided with three repetitions, namely each repetition is 4 mu, the area of the blank control area is 2 mu, and the wheat variety is stone 4366.
Table 1 test treatment protocol
b. Investigation time and method
Investigating the effect of the wheat stem base rot: the study is carried out once in the turning green and jointing stage, heading stage and milk stage, sampling is carried out at five points, 20 plants are studied at each point, and the disease severity is recorded according to the following grading method:
stage 0: the innermost leaf sheath and the whole stem of the plant have no browning symptom;
stage 1: the leaf sheath at the innermost layer of the overground part is obviously browned, and the first stem node is not obviously browned;
and 3, level: the first part of the overground stem nodes has browning phenomenon;
and 5, stage: browning of the second part of the above-ground stem nodes;
and 7, stage: the third part of the overground stem node has browning phenomenon but does not have withered white ears;
and 9, stage: the lesion spots exceed the third stem node, or white ears exist or no ears exist due to the disease.
Respectively investigating wheat sharp eyespot once in the jointing stage and the withered and white ear manifestation stage, repeatedly sampling five points each, investigating 20 plants each, recording the severity of the disease condition according to the following grading method:
level 0: the disease is not developed;
level 1: the leaf sheath is diseased but the stem is not diseased;
and 3, stage: the leaf sheath is diseased and invades the stem, but the lesion of the stem and the annular stem are less than 1/2;
and 5, stage: the stigmata miniata stems exceed 1/2, but do not fall or break;
and 7, stage: withering, lodging, white ear.
The drug effect calculation method comprises the following steps:
disease rate (%) = (number of diseased plants/total number of investigated plants) × 100;
disease index = [ Σ (number of diseased plants at each level × relative level value)/(total number of investigated plants × highest level) ] × 100;
control effect = [ (disease finger in blank control area-disease finger in medicament treatment area)/disease finger in blank control area ] × 100.
And (3) yield survey: 5 days before harvest, taking 2 points per repetition point, taking 1 square meter per point, recording the total spike number, the dry spike weight and the total grain weight per point, and recording the yield per mu (kg) = the average total grain weight per square meter (g) × 666.7/1000
c. Results and analysis
The control effect of each treatment on the basal stem rot of wheat can be seen in table 2, and the investigation of the jointing stage, the heading stage and the milk stage shows that the effect of 0.4 percent of verticillium dahliae activator protein wettable powder seed dressing and leaf surface spraying (treatment 2) is the best, and the control effects of the three-stage investigation are 98.06 percent, 97.09 percent and 98.60 percent; the 0.4% verticillium dahliae activator protein wettable powder has the inferior seed dressing effect (treatment 1), and the control effects are 96.12%, 88.36% and 95.79% in three-period investigation; the spraying effect of 0.4% of the verticillium dahliae activator protein wettable powder (treatment 3) is that the control effect is 74.60%, 83.51% and 90.88% in three-stage investigation; the conventional control effect (treatment 4) was found to be 68.78%, 72.66% and 88.92% in three periods.
TABLE 2 survey and record table of stem base rot
The control effect of each treatment on the wheat sharp eyespot is shown in table 3, and the investigation of the elongation stage and the milk stage shows that the 0.4 percent verticillium dahliae activated protein wettable powder has the best effect of seed dressing and leaf surface spraying (treatment 2), and the control effect is 86.15 percent and 82.02 percent; the 0.4% verticillium dahliae activator protein wettable powder is mixed with seeds for a second time (treatment 1), and the control effect is 82.30% and 70.00%;0.4% of verticillium dahliae activator protein wettable powder is sprayed (treatment 3), and the control effect is 70.64% and 69.68%; the conventional control effects were 66.67%, 60.67% (treatment 4).
TABLE 3 banded sclerotial blight investigation record chart
The wheat yield is determined in attached table 4, the wheat yield is determined by sampling, the yield of 0.4% verticillium dahliae activated protein wettable powder is the highest by seed dressing and leaf surface spraying (treatment 2), 669.3 kilograms is achieved, the yield is increased by 161.3 kilograms compared with a blank control, the yield increase rate is 31.7 percent, the yield is increased by 48 kilograms compared with a conventional control, and the yield increase rate is 7.7 percent; the yield of 0.4 percent of the verticillium dahliae activator protein wettable powder treated by seed dressing is lower than that of the treatment (treatment 1), the yield reaches 657.5 kilograms, is increased by 149.5 kilograms compared with a blank control, the yield is increased by 29.4 percent, is increased by 36.2 kilograms compared with a conventional control, and the yield is increased by 5.8 percent; the yield per mu of 0.4% verticillium dahliae activated protein wettable powder after spraying treatment reaches 628.2 kilograms (treatment 3), the yield is increased by 120.2 kilograms compared with a blank control, the yield is increased by 23.7%, and the yield is increased by 6.9 kilograms compared with a conventional control and is increased by 1.1%; the yield per mu treated by the conventional control method is 621.3 kg, and is increased by 22.3 percent compared with a blank control.
TABLE 4 wheat yield record sheet
The embodiments in the present specification are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar embodiments may be referred to each other.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (9)
1. The wettable powder containing the verticillium dahliae activator protein is characterized by comprising the following raw materials in parts by mass per 100: 5-20 parts of verticillium dahliae activated protein raw powder, 5-10 parts of wetting agent, 2-6 parts of dispersing agent, 5-15 parts of stabilizing agent and the balance of filler.
2. The wettable powder of claim 1 wherein the wetting agent: one or two of sodium dodecyl benzene sulfonate and sodium dibutyl naphthalene sulfonate.
3. The wettable powder of claim 2 wherein the dispersant is one or a mixture of alkylnaphthalene sulfonate, dialkylnaphthalene sulfonate formaldehyde condensate and lignosulfonate.
4. A wettable powder as claimed in claim 3, wherein the stabilizer: one or more of sodium dihydrogen phosphate/potassium dihydrogen phosphate, disodium hydrogen phosphate/potassium hydrogen phosphate, disodium ethylene diamine tetraacetic acid and ascorbic acid.
5. The wettable powder of claim 4, wherein the filler is one or more of light calcium carbonate, bentonite, white carbon black, anhydrous sodium sulphate and ammonium salt.
6. A preparation method of wettable powder containing verticillium dahliae activator protein is characterized in that the raw materials of any one of claims 1 to 5 are uniformly mixed and are pulverized to 300-500 meshes by air flow, and the wettable powder containing the verticillium dahliae activator protein is obtained.
7. Use of a wettable powder according to any one of claims 1 to 6 in agricultural production.
8. The use of claim 7, wherein: it can be used for wheat to resist diseases and increase yield.
9. The use of claim 8, wherein the disease comprises: wheat stem rot and wheat sheath blight.
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1493194A (en) * | 2003-08-18 | 2004-05-05 | 邱德文 | Fungus biological protein wettable powder agent |
| WO2006090902A1 (en) * | 2005-02-24 | 2006-08-31 | Sumitomo Chemical Company, Limited | Composition for controlling harmful organism |
| CN105198967A (en) * | 2015-10-22 | 2015-12-30 | 中国农业大学 | Application of protein VdAL to improvement of plant product quality |
| CN105218649A (en) * | 2015-10-22 | 2016-01-06 | 中国农业大学 | Protein VdAL is improving the application in plant biomass and Promoting plant growth |
| CN105875651A (en) * | 2014-10-17 | 2016-08-24 | 中国农科院植保所廊坊农药中试厂 | Application of activator protein prepared from Alternaria tenuissima by microbial fermentation to pesticide preparation |
| CN108719337A (en) * | 2018-05-30 | 2018-11-02 | 崔明华 | A kind of drug and its production method for curing plant cancer blight |
| CN110343151A (en) * | 2019-07-17 | 2019-10-18 | 中国农业科学院农产品加工研究所 | A kind of purposes of verticillium dahliae effect protein VdSCP113 |
| CN114600880A (en) * | 2022-02-08 | 2022-06-10 | 南京农业大学 | Wettable powder of PopW protein and application thereof |
-
2022
- 2022-09-20 CN CN202211145286.3A patent/CN115413678A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1493194A (en) * | 2003-08-18 | 2004-05-05 | 邱德文 | Fungus biological protein wettable powder agent |
| WO2006090902A1 (en) * | 2005-02-24 | 2006-08-31 | Sumitomo Chemical Company, Limited | Composition for controlling harmful organism |
| CN105875651A (en) * | 2014-10-17 | 2016-08-24 | 中国农科院植保所廊坊农药中试厂 | Application of activator protein prepared from Alternaria tenuissima by microbial fermentation to pesticide preparation |
| CN105198967A (en) * | 2015-10-22 | 2015-12-30 | 中国农业大学 | Application of protein VdAL to improvement of plant product quality |
| CN105218649A (en) * | 2015-10-22 | 2016-01-06 | 中国农业大学 | Protein VdAL is improving the application in plant biomass and Promoting plant growth |
| CN108719337A (en) * | 2018-05-30 | 2018-11-02 | 崔明华 | A kind of drug and its production method for curing plant cancer blight |
| CN110343151A (en) * | 2019-07-17 | 2019-10-18 | 中国农业科学院农产品加工研究所 | A kind of purposes of verticillium dahliae effect protein VdSCP113 |
| CN114600880A (en) * | 2022-02-08 | 2022-06-10 | 南京农业大学 | Wettable powder of PopW protein and application thereof |
Non-Patent Citations (5)
| Title |
|---|
| 丰汇华农: "极细链格孢激活蛋白", vol. 31, no. 10, pages 58 * |
| 张婷婷 等: "植物免疫诱抗剂"维大力"在小麦生产上的应用效果初探", 《乡村科技》, vol. 14, no. 1, pages 80 - 83 * |
| 赵磊 等: "植物激活蛋白对草莓白粉病的治疗和预防效果研究", no. 4, pages 55 * |
| 闻建波 等: "蛋白制剂维大利（VDAL）不同施用方式对小麦抗病性及产量的影响", 《农业科技通讯》, no. 2 * |
| 龚新姿: "蛋白质VdAL施用对水稻产量影响的示范试验", vol. 40, no. 08, pages 11 - 12 * |
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