CN110343151A - A kind of purposes of verticillium dahliae effect protein VdSCP113 - Google Patents
A kind of purposes of verticillium dahliae effect protein VdSCP113 Download PDFInfo
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- CN110343151A CN110343151A CN201910646907.8A CN201910646907A CN110343151A CN 110343151 A CN110343151 A CN 110343151A CN 201910646907 A CN201910646907 A CN 201910646907A CN 110343151 A CN110343151 A CN 110343151A
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- plant
- vdscp113
- effect protein
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
- A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
- A01N47/44—Guanidine; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
Abstract
This disclosure relates to a kind of purposes of verticillium dahliae effect protein VdSCP113, which includes: effect protein VdSCP113 for improving plant resistance to environment stress and/or induction plant defense response.When effect protein VdSCP113 is used to improve plant resistance to environment stress and induction plant defense response by the disclosure, the efficiency of the immune responses such as induction ROS outburst, callose deposition is higher, and plant resistance to environment stress improves and defense reaction inducing effect is obvious.The disclosure provides new approach to improve plant resistance to environment stress and induction plant defense response, has broad application prospects in agricultural production.
Description
Technical field
This disclosure relates to microprotein applied technical field, and in particular to effect protein VdSCP113 is improving Genes For Plant Tolerance
Property and induction plant defense response in application.
Background technique
Pathogen-associated molecular pattern (PAMPs) refers to the certain shared highly conserved molecule knots in pathogenic microorganism surface
Structure, usually pathogenic microorganism institute is peculiar, and host cell does not generate, and is the existence or pathogenic necessary of microorganism.It plants
Object can identify PAMPs molecule by the pattern recognition receptors (PRRs) on cell membrane, thus the pathogen correlation of activated plant point
Subpattern triggers immune (PTI), to resist infecting for pathogenic microorganism.After plants identification PAMPs molecule, it can generate a series of
Defense reaction, such as Ca in cytoplasm2+The variation of concentration, the activation of MAPKs kinase signal pathway, ROS outburst, cytoplasm acidification, ethylene
Synthesis, the expression of pathogenesis related gene, cell wall callose precipitating, the synthesis of phytoalexin, cell plasma leakage and generation
HR reaction etc..
ROS outburst plays very important effect in the plant disease-resistant defense response that PAMPs molecule induces, it participates in adjusting
Stomatal movement is controlled, promotes Apoptosis, directly or indirectly kills the pathogen of invasion, can transcribed as second messenger
The expression of various defense response genes in plant and the foundation etc. of participation systemic acquired resistance are activated and regulated and controled in level.
Cell wall callose precipitating, can reinforce plant cell wall to prevent pathogenic microorganism from invading, and prevent most of pathogenic microorganisms fixed
It grows, to achieve the purpose that resist defence pathogenic microorganism.
But the induced efficiency of the immune responses such as ROS outburst at present, callose deposition is still lower.
Summary of the invention
The purpose of the disclosure is to improve the induced efficiency of the substances of immune responses such as induction ROS outburst, callose deposition.
To achieve the goals above, the disclosure provides the purposes of verticillium dahliae effect protein VdSCP113 a kind of, described
For effect protein VdSCP113 amino acid sequence as shown in SEQ ID NO:2, the purposes includes: the effect protein VdSCP113
For improving plant resistance to environment stress and/or induction plant defense response.
Optionally, the raising plant resistance to environment stress, comprising:
It is sick at least one of the pathogen of Botrytis cinerea, phytophthora blight of pepper, verticillium dahliae, Fusariumsp and leaf mycete to promote plant
The resistance of substance；
The induction plant defense response, comprising:
Induction reduces at least one of the pathogen of Botrytis cinerea, phytophthora blight of pepper, verticillium dahliae, Fusariumsp and leaf mycete cause of disease
Harm of the body to plant.
Optionally, the plant includes at least one of tobacco, cotton, capsicum, arabidopsis, potato and tomato.
Optionally, the application concentration of the effect protein VdSCP113 is 0.001~10 μM.
The disclosure additionally provides a kind of method for improving plant resistance to environment stress and/or inducing plant defense response, this method comprises:
The encoding gene of effect protein VdSCP113 is imported into purpose plant, resistance is obtained and improves and/or occur defence instead
The plant answered；Alternatively,
Effect protein VdSCP113 is applied on purpose plant, the plant that resistance improves and/or defense reaction occurs is obtained
Strain；
Wherein, the polynucleotide sequence of the encoding gene of the effect protein VdSCP113 is as shown in SEQ ID NO:1；Institute
Effect protein VdSCP113 amino acid sequence is stated as shown in SEQ ID NO:2.
Optionally, the encoding gene of the effect protein VdSCP113 imports purpose plant by plant expression vector.
Optionally, the plant expression vector includes Ti class plasmid vector and/or viral vectors.
Optionally, the plant includes at least one of tobacco, cotton, capsicum, arabidopsis, potato and tomato.
The disclosure additionally provides effect protein VdSCP113 and is preparing the application in pesticide, and the pesticide is for improving plant
Resistance and/or induction plant defense response, the effect protein VdSCP113 amino acid sequence is as shown in SEQ ID NO:2.
Optionally, the plant includes at least one of tobacco, cotton, capsicum, arabidopsis, potato and tomato.
Through the above technical solutions, being used to improve plant resistance to environment stress and induction plant defense response for effect protein VdSCP113
When, the efficiency of the immune responses such as induction ROS outburst, callose deposition is higher, and plant resistance to environment stress improves and defense reaction inducing effect
Obviously.The disclosure provides new approach to improve plant resistance to environment stress and induction plant defense response, has in agricultural production wide
Wealthy application prospect.
Other feature and advantage of the disclosure will the following detailed description will be given in the detailed implementation section.
Detailed description of the invention
Attached drawing is and to constitute part of specification for providing further understanding of the disclosure, with following tool
Body embodiment is used to explain the disclosure together, but does not constitute the limitation to the disclosure.In the accompanying drawings:
Fig. 1 is the experimental result picture that effect protein VdSCP113 induces tobacco leaf ROS reaction in the embodiment of the present disclosure 1.
Fig. 2 is the experimental result that effect protein VdSCP113 induces the accumulation of tobacco leaf callose in the embodiment of the present disclosure 1
Figure.
Fig. 3 is that effect protein VdSCP113 improves tobacco defense reaction related gene expression level in the embodiment of the present disclosure 1
Experimental result picture.
Fig. 4 be in the embodiment of the present disclosure 2 effect protein VdSCP113 evoking tobacco to the reality of the Resistant reaction of Botrytis cinerea
Test result figure.
Specific embodiment
The specific embodiment of the disclosure is described in detail below.It should be understood that described herein specific
Embodiment is only used for describing and explaining the disclosure, is not limited to the disclosure.
The first aspect of the disclosure provides the purposes of verticillium dahliae effect protein VdSCP113 a kind of, the effect protein
For VdSCP113 amino acid sequence as shown in SEQ ID NO:2, the purposes includes: the effect protein VdSCP113 for improving
Plant resistance to environment stress and/or induction plant defense response.
Through the disclosed invention people the study found that after the encoding gene for knocking out VdSCP113 in verticillium dahliae, to mutation
The pathogenesis phenotype of bacterial strain is identified that qualification result shows to knock out the verticillium dahliae mutant bacteria after VdSCP113 encoding gene
Strain does not have significant changes to cotton and the pathogenic of tobacco, illustrates that VdSCP113 is not the virulence factor of verticillium dahliae；It will
After the effect protein VdSCP113 injection plant leaf blade of verticillium dahliae, such as tobacco leaf, it can detect ROS's in blade
Generate and the accumulation of callose, and the expression for being injected some defense reaction related genes of plant it is also significant on
It adjusts, such as HR reaction marking gene HSR203, H1N1 and GRAS2；Pathogenesis related gene PR1, PR4 and Glnb.Therefore, it can will imitate
Answer albumen VdSCP113 for improving plant resistance to environment stress and/or induction plant defense response.
In above-mentioned technical proposal, effect protein VdSCP113 is used to improve plant resistance to environment stress and induction plant defense response
When, the efficiency of the immune responses such as induction ROS outburst, callose deposition is higher, and plant resistance to environment stress improves and defense reaction inducing effect
Obviously.The disclosure provides new approach to improve plant resistance to environment stress and induction plant defense response, has in agricultural production wide
Wealthy application prospect.
According to the disclosure, improving plant resistance to environment stress includes the resistance for improving purpose plant pair pathogen infection, can mitigate disease
Substance limits pathogen and further spreads in purpose plant to the gradient of infection of purpose plant.Disclosure meaning pathogen packet
Virus, bacterium, oomycetes, fungi and nematode etc. are included, the type of the pathogen can select in a wider scope.Preferably,
Effect protein VdSCP113 can promote plant in the pathogen of Botrytis cinerea, phytophthora blight of pepper, verticillium dahliae, Fusariumsp and leaf mycete
At least one pathogen resistance.
According to the disclosure, inducing plant defense response includes that induction purpose plant activates autoprotcrtive system, to reduce disease
Pathogens are endangered caused by purpose plant, and the type of the pathogen can select in a wider scope.Preferably, it imitates
It answers albumen VdSCP113 can induce to reduce in the pathogen of Botrytis cinerea, phytophthora blight of pepper, verticillium dahliae, Fusariumsp and leaf mycete at least
A kind of harm of pathogen to plant.
According to the disclosure, the type of the plant can select in a wider scope.As preferable case, the plant
It may include at least one of tobacco, cotton, capsicum, arabidopsis, potato and tomato.Effect protein VdSCP113 is to above-mentioned
The resistance of plant in preferred scope improves and the effect of defense reaction induction is more preferable.
According to the disclosure, the application concentration of effect protein VdSCP113 can select in a wider scope.Disclosure effect
The application concentration for answering albumen VdSCP113 can be 0.001~10 μM, preferably 0.01~1 μM.In above-mentioned preferred concentration range
Interior, effect protein VdSCP113 can faster, preferably improve plant resistance to environment stress and/or induction plant defense response.
The second aspect of the disclosure provides a kind of method for improving plant resistance to environment stress and/or inducing plant defense response, should
Method includes:
The encoding gene of effect protein VdSCP113 is imported into purpose plant, resistance is obtained and improves and/or occur defence instead
The plant answered；Alternatively,
Effect protein VdSCP113 is applied on purpose plant, the plant that resistance improves and/or defense reaction occurs is obtained
Strain；
Wherein, the polynucleotide sequence of the encoding gene of the effect protein VdSCP113 is as shown in SEQ ID NO:1；Institute
Effect protein VdSCP113 amino acid sequence is stated as shown in SEQ ID NO:2.
The encoding gene of effect protein VdSCP113 is imported into purpose plant, the encoding gene wink of effect protein VdSCP113
When transfect purpose plant, and express generate effect protein VdSCP113；Alternatively, directly by effect protein VdSCP113 or containing effective
It answers the composition of albumen VdSCP113 to import purpose plant, after plants identification effect protein VdSCP113, can generate a series of
Defense reaction, such as the expression up-regulation of the generation of ROS, the accumulation of callose and defense reaction related gene etc., to reach
To the purpose for improving plant resistance to environment stress and/or induction plant defense response.Disclosed method process is relatively easy and is easy to control.
According to the disclosure, under preferable case, the encoding gene of effect protein VdSCP113 can pass through plant expression vector
Import purpose plant.Encoding gene is imported by plant expression vector, method is simple, quick, and success rate is relatively high.
According to the disclosure, the type of the plant expression vector can select in a wider scope.Under preferable case, plant
Object expression vector may include Ti class plasmid vector and/or viral vectors.Above-mentioned carrier is easy to get, and cost is relatively low, and above-mentioned
Encoding gene is imported and transfects having higher success rate for purpose plant by the easy to carry encoding gene of carrier.
According to the disclosure, the type of the plant can select in a wider scope.As preferable case, the plant
It may include at least one of tobacco, cotton, capsicum, arabidopsis, potato and tomato.Method of disclosure is to above-mentioned plant
Resistance improves and the effect of defense reaction induction is more preferable.
The third aspect of the disclosure provides effect protein VdSCP113 and is preparing the application in pesticide, and the pesticide is for mentioning
High plant resistance to environment stress and/or induction plant defense response, the effect protein VdSCP113 amino acid sequence such as SEQ ID NO:2 institute
Show.
Pesticide can be made in effect protein VdSCP113 and other acceptable auxiliary agents, and be used for the pesticide to improve plant
Resistance and/or induction plant defense response.The disclosure is that preparation improves plant resistance to environment stress and the pesticide of plant defense response is induced to mention
It has supplied new raw medicine to select, has had broad application prospects in pesticide producing.
According to the disclosure, the type of the plant can select in a wider scope.As preferable case, the plant
It may include at least one of tobacco, cotton, capsicum, arabidopsis, potato and tomato.The disclosure contains effect protein
The pesticide of VdSCP113 improves the resistance of above-mentioned plant and the effect of defense reaction induction is more preferable.
The disclosure is further illustrated below by embodiment, but therefore the disclosure is not any way limited.
Effect protein VdSCP113 involved in the embodiment of the present disclosure, amino acid sequence is as shown in SEQ ID NO:2.Effect
Answer albumen VdSCP113's the preparation method comprises the following steps: building recombinant expression carrier, utilize Escherichia coli carry out prokaryotic expression, expression vector
In have maltose-binding protein MBP label, purified using MBP TrapHP prepacked column, obtain destination protein.
1 effect protein VdSCP113 of embodiment excites tobacco immune response
(1) effect protein VdSCP113 induces tobacco leaf ROS reaction
With 1mL without the syringe of syringe needle, will about 20 μ L, 100nM effect protein VdSCP113, injected from vacuum side of blade
In 3~4 week old tobacco leafs.Negative control is used as with the Tris-HCl (20nM, pH8.0) of same concentrations.After injecting 6h, it will locate
The tobacco leaf of reason is rinsed well with deionized water, is placed in DAB dye liquor, is protected from light in 25 DEG C and is incubated for 8h, is removed dye liquor and will be contaminated
After tobacco leaf after color is rinsed well with deionized water, with 95% ethanol decolorization, is observed and taken pictures using Stereo microscope.
As a result: the tobacco leaf of injecting effect albumen VdSCP113, injection areas have a large amount of H2O2Accumulation；Injection
The tobacco leaf of Tris-HCl, injection areas H2O2Accumulation it is extremely faint, as shown in Figure 1.
The above results explanation, effect protein VdSCP113 can induce tobacco leaf ROS reaction.
(2) effect protein VdSCP113 induces the accumulation of tobacco leaf callose
With 1mL without the syringe of syringe needle, will about 20 μ L, 100nM effect protein VdSCP113, injected from vacuum side of blade
In 3~4 week old tobacco leafs.Negative control is used as with the Tris-HCl (20nM, pH8.0) of same concentrations.After injecting 2d, it will infuse
It penetrates after area's blade is washed with deionized water, rinses remaining ethyl alcohol well with 95% ethanol decolorization, then with deionized water, by leaf
Piece is placed in the 150mM phosphate buffer containing 0.1% (W/V) aniline blue of pH9.5, dark treatment 2h, then takes out blade utilization
Fluorescence microscope is simultaneously taken pictures.
As a result: the tobacco leaf of injecting effect albumen VdSCP113, injection areas have a large amount of blue spot；Injection
The tobacco leaf of Tris-HCl, injection areas only have only a few blue spot, as shown in Figure 2.
The above results explanation, effect protein VdSCP113 can induce the accumulation of tobacco leaf callose.
(3) it is horizontal to improve tobacco defense reaction related gene expression by effect protein VdSCP113
With 1mL without the syringe of syringe needle, will about 20 μ L, 100nM effect protein VdSCP113, injected from vacuum side of blade
In 4 week old tobacco leafs.Negative control is used as with the Tris-HCl (20nM, pH8.0) of same concentrations.After injecting 12h, note is collected
Region blade is penetrated, RNA, reverse transcription cDNA are extracted, the expression of defense reaction related gene is detected by RT-PCR.
As a result: the tobacco leaf of injecting effect albumen VdSCP113, HR reaction marking gene HSR203, H1N1 and GRAS2；
Pathogenesis related gene PR1, PR4 and Glnb are by a degree of inducing expression.Experimental result is as shown in Figure 3.
The above results explanation, effect protein VdSCP113 can effectively improve the expression of tobacco defense-related gene.
Resistant reaction of the 2 effect protein VdSCP113 evoking tobacco of embodiment to pathogen
With 1mL without the syringe of syringe needle, will about 20 μ L, 100nM effect protein VdSCP113, injected from vacuum side of blade
In 4 week old tobacco leafs.Negative control is used as with the Tris-HCl (20nM, pH8.0) of same concentrations.After injecting 12h-24h, use
Syringe needle is carried out pricking hole and be handled in injection areas, the 5 μ L that will be prepared in advance, and 5 × 106The difference pathogen to be measured of spores/mL
Spore suspension drop is cultivated under 25 DEG C, 80% damp condition right above pinprick, is observed after being inoculated with 3-5d and is measured each scab
Area.Experimental result is as shown in Figure 4.Inhibition feelings of the effect protein VdSCP113 to tobacco leaf scab caused by each pathogen
Condition is shown in Table 1.
Inhibition situation of the 1 effect protein VdSCP113 of table to tobacco leaf scab caused by each pathogen
The above results explanation, effect protein VdSCP113 can obvious evoking tobacco to the pathogen of Botrytis cinerea, phytophthora blight of pepper, big
Beautiful Verticillium dahliae, Fusariumsp and leaf mycete Resistant reaction.
3 effect protein VdSCP113 of embodiment induces different plants to the Resistant reaction of the pathogen of Botrytis cinerea
Effect protein VdSCP113 spray solution or root irrigation different experiments group plant using concentration about 100nM, often
Group experiment leaf portion sprays 1~2mL or the above-mentioned protein solution of 10~15mL of pouring root, and experiment is 20 plants with the quantity of plant.With identical
The Tris-HCl (20nM, pH8.0) of concentration is used as negative control.Processing about for 24 hours after, pouring root inoculation 20mL prepare in advance 5
×106The pathogen of Botrytis cinerea spore suspension of spores/mL, is cultivated under 25 DEG C, 80% damp condition, and inoculation 30d observation is simultaneously
Count the incidence of each experimental group plant.
Dividing state of an illness grade according to incidence is 0 grade, 1 grade, 2 grades, 3 grades, 4 grades, the incidence of different state of an illness grades
Are as follows:
0 grade: plant leaf blade is disease-free or almost without morbidity；
There is symptom in 1 grade: 25% of blade；
There is symptom in 2 grades: 26%~50% of blade；
There is symptom in 3 grades: 51%~75% of blade；
There is symptom in 4 grades: 76%~100% of blade.
Different experiments group plant difference state of an illness grade proportion (%) is as shown in table 2.
2 different experiments group plant difference state of an illness grade proportion (%) of table
The above results explanation, effect protein VdSCP113 can obviously induce capsicum, cotton, tomato, potato and arabidopsis
To the Resistant reaction of the pathogen of Botrytis cinerea.
The preferred embodiment of the disclosure is described in detail in conjunction with attached drawing above, still, the disclosure is not limited to above-mentioned reality
The detail in mode is applied, in the range of the technology design of the disclosure, a variety of letters can be carried out to the technical solution of the disclosure
Monotropic type, these simple variants belong to the protection scope of the disclosure.
It is further to note that specific technical features described in the above specific embodiments, in not lance
In the case where shield, can be combined in any appropriate way, in order to avoid unnecessary repetition, the disclosure to it is various can
No further explanation will be given for the combination of energy.
In addition, any combination can also be carried out between a variety of different embodiments of the disclosure, as long as it is without prejudice to originally
Disclosed thought equally should be considered as disclosure disclosure of that.
Sequence table
<110>Institute of Agricultural Product Processing, Chinese Academy of Agricultural Sc
<120>a kind of purposes of verticillium dahliae effect protein VdSCP113
<130> 13629CAAS-F-CJY
<160> 2
<170> SIPOSequenceListing 1.0
<210> 1
<211> 576
<212> DNA
<213>verticillium dahliae (Verticillium dahliae)
<400> 1
atgaagttct ccagcctctt tctgcctacc gtcttcgtct ccgccgtgta cggcaacggc 60
tttgtcgaga gacgcgcgac caagcaatgc aatcccggaa ataactgcca acgcggggtc 120
ggaggaacag ccggcgtgag gcctcctctg actagccgtc aggccgactg ctcaaggctc 180
aatgtcgtga cagtcagccc tttcactact accacaaccc tcacaacgac cgtcaccatc 240
gtcgacatcc cgaatccgac aagcctcaag cgtgccgtga ctctccctac cgccggggtt 300
gcgaagcgtc aggatgtcgg tgccgccacc atcacgccca cagcagttcc cacctacgcg 360
acatactgct cctccgcctc ggcctactac agcgcctgtt cgtgcgctgg catcacggct 420
gtcaccacca ctctgcctac gcctaccgcg accgcgtaca gtaccgtctc ggtctgcgcc 480
gccttgaatc tggctcgcag ggcgctggag ggatttggct acgaggttga gcctgatttc 540
gacgccatca agtatcccgg attcagactt atctga 576
<210> 2
<211> 191
<212> PRT
<213>verticillium dahliae (Verticillium dahliae)
<400> 2
Met Lys Phe Ser Ser Leu Phe Leu Pro Thr Val Phe Val Ser Ala Val
1 5 10 15
Tyr Gly Asn Gly Phe Val Glu Arg Arg Ala Thr Lys Gln Cys Asn Pro
20 25 30
Gly Asn Asn Cys Gln Arg Gly Val Gly Gly Thr Ala Gly Val Arg Pro
35 40 45
Pro Leu Thr Ser Arg Gln Ala Asp Cys Ser Arg Leu Asn Val Val Thr
50 55 60
Val Ser Pro Phe Thr Thr Thr Thr Thr Leu Thr Thr Thr Val Thr Ile
65 70 75 80
Val Asp Ile Pro Asn Pro Thr Ser Leu Lys Arg Ala Val Thr Leu Pro
85 90 95
Thr Ala Gly Val Ala Lys Arg Gln Asp Val Gly Ala Ala Thr Ile Thr
100 105 110
Pro Thr Ala Val Pro Thr Tyr Ala Thr Tyr Cys Ser Ser Ala Ser Ala
115 120 125
Tyr Tyr Ser Ala Cys Ser Cys Ala Gly Ile Thr Ala Val Thr Thr Thr
130 135 140
Leu Pro Thr Pro Thr Ala Thr Ala Tyr Ser Thr Val Ser Val Cys Ala
145 150 155 160
Ala Leu Asn Leu Ala Arg Arg Ala Leu Glu Gly Phe Gly Tyr Glu Val
165 170 175
Glu Pro Asp Phe Asp Ala Ile Lys Tyr Pro Gly Phe Arg Leu Ile
180 185 190
Claims (10)
1. a kind of purposes of verticillium dahliae effect protein VdSCP113, the effect protein VdSCP113 amino acid sequence is such as
Shown in SEQ ID NO:2, which is characterized in that the purposes includes: the effect protein VdSCP113 for improving plant resistance to environment stress
And/or induction plant defense response.
2. the purposes of verticillium dahliae effect protein VdSCP113 as described in claim 1, which is characterized in that the raising is planted
Object resistance, comprising:
Plant is promoted at least one of the pathogen of Botrytis cinerea, phytophthora blight of pepper, verticillium dahliae, Fusariumsp and leaf mycete pathogen
Resistance；
The induction plant defense response, comprising:
Induction reduces at least one of the pathogen of Botrytis cinerea, phytophthora blight of pepper, verticillium dahliae, Fusariumsp and leaf mycete pathogen pair
The harm of plant.
3. the purposes of verticillium dahliae effect protein VdSCP113 as claimed in claim 1 or 2, which is characterized in that the plant
Object includes at least one of tobacco, cotton, capsicum, arabidopsis, potato and tomato.
4. the purposes of verticillium dahliae effect protein VdSCP113 as claimed in claim 1 or 2, which is characterized in that the effect
The application concentration for answering albumen VdSCP113 is 0.001~10 μM.
5. a kind of method for improving plant resistance to environment stress and/or inducing plant defense response, which is characterized in that this method comprises:
The encoding gene of effect protein VdSCP113 is imported into purpose plant, resistance is obtained and improves and/or occur defense reaction
Plant；Alternatively,
Effect protein VdSCP113 is applied on purpose plant, the plant that resistance improves and/or defense reaction occurs is obtained；
Wherein, the polynucleotide sequence of the encoding gene of the effect protein VdSCP113 is as shown in SEQ ID NO:1；The effect
Answer albumen VdSCP113 amino acid sequence as shown in SEQ ID NO:2.
6. method as claimed in claim 5, which is characterized in that the encoding gene of the effect protein VdSCP113 passes through plant
Expression vector imports purpose plant.
7. method as claimed in claim 6, which is characterized in that the plant expression vector include Ti class plasmid vector and/or
Viral vectors.
8. the method as described in any one of claim 5-7, which is characterized in that the plant include tobacco, cotton, capsicum,
At least one of arabidopsis, potato and tomato.
9. effect protein VdSCP113 is preparing the application in pesticide, the pesticide is for improving plant resistance to environment stress and/or induction plant
Object defense reaction, the effect protein VdSCP113 amino acid sequence is as shown in SEQ ID NO:2.
10. effect protein VdSCP113 as claimed in claim 9 is preparing the application in pesticide, which is characterized in that the plant
Object includes at least one of tobacco, cotton, capsicum, arabidopsis, potato and tomato.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910646907.8A CN110343151B (en) | 2019-07-17 | 2019-07-17 | Application of verticillium dahliae effector protein VdSCP113 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910646907.8A CN110343151B (en) | 2019-07-17 | 2019-07-17 | Application of verticillium dahliae effector protein VdSCP113 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110343151A true CN110343151A (en) | 2019-10-18 |
| CN110343151B CN110343151B (en) | 2021-04-16 |
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Cited By (2)
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| CN110885849A (en) * | 2019-12-05 | 2020-03-17 | 沈阳农业大学 | Recombinant vector, host cell and application of Ustilaginoidea virens effector protein |
| CN115413678A (en) * | 2022-09-20 | 2022-12-02 | 中捷四方生物科技股份有限公司 | Wettable powder containing verticillium dahliae activator protein and application |
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| CN105255742A (en) * | 2015-10-22 | 2016-01-20 | 山东农业大学 | Endogenous penicillium capable of antagonizing four fusarium fungi and application thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110885849A (en) * | 2019-12-05 | 2020-03-17 | 沈阳农业大学 | Recombinant vector, host cell and application of Ustilaginoidea virens effector protein |
| CN115413678A (en) * | 2022-09-20 | 2022-12-02 | 中捷四方生物科技股份有限公司 | Wettable powder containing verticillium dahliae activator protein and application |
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