CN110331180A - One boar placenta polypeptide production method - Google Patents
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Abstract
The present invention provides a boar placenta polypeptide production methods, belong to food processing technology field.The present invention will first blend after the cleaning of pig placenta, obtain pig placenta meat gruel；Then the pig placenta meat gruel is mixed with the water of 2~10 times of pig placenta meat gruel quality, obtains meat gruel liquid；The meat gruel liquid is mixed with the complex enzyme of 0.5%~10% pig placenta meat gruel quality again, obtains mixed liquor；Radiation sterilizing after mixed liquor vacuum packaging, then enzyme digestion reaction 4~for 24 hours, obtains enzymolysis liquid；It will be filtered after enzymolysis liquid centrifugation again, obtain supernatant；Finally the supernatant is spray-dried, obtains pig placenta polypeptide.Method high income provided by the invention, the period is short, pollution-free, and the pig placenta polypeptide being prepared using the method for the invention can be developed into functional beverage and health product, polypeptide products fishy smell is light, color just.
Description
Technical field
The present invention relates to technical field of food production, and in particular to a boar placenta polypeptide production method.
Background technique
Placenta also known as " dried human placenta " are a kind of special functional organization's organs.Modern biology and medical research prove: pig
Placenta contains biologically active peptide, immunoglobulin, amino acid, minerals, cell factor, growth factor, phosphatide, polysaccharide etc.." this
Careless detailed outline " record: placenta " warm-natured, position is sweet salty, enter lung, enter liver, kidney three pass through, have tranquilizing and blood nourishing, tonifying Qi, strengthening the essence, removing toxic substances,
The effect of enriching blood has fancy effect to tired, thin, languisher, and long term usage is then clear-headed and clear-sighted, and beard and hair is black, promotes longevity, and has to take by force and make
The effect of change ".Therefore either from the point of view of modern medicine angle, or from traditional Chinese medicine angle, pig placenta and its extract
All there is great development and application value.The extraction of pig placenta polypeptide at present mainly utilizes mode of action, recovery rate is low,
The problem of easily causing microbial contamination in enzymolysis time length, enzymolysis process, using pepsin and alkali protease to equipment corruption
Corrosion is unfavorable for greatly industrialized production.
Summary of the invention
The problem of in view of background technique, the purpose of the present invention is to provide a kind of reaction conditions mildly, yield
Height, the period is short, pollution-free, the pig placenta polypeptide production method that polypeptide products fishy smell is light, color is positive.
The present invention provides following technical schemes:
One boar placenta polypeptide production method, includes the following steps:
(1) it will be blended after the cleaning of pig placenta, obtain pig placenta meat gruel；
(2) the pig placenta meat gruel is mixed with the water of 2~10 times of pig placenta meat gruel quality, obtains meat gruel liquid；
(3) the meat gruel liquid is mixed with the complex enzyme of 0.5%~10% pig placenta meat gruel quality, obtains mixed liquor；It is mixed
Radiation sterilizing after liquid vacuum packaging is closed, then enzyme digestion reaction 4~for 24 hours, obtains enzymolysis liquid；
(4) it will be filtered after enzymolysis liquid centrifugation, obtain supernatant；
(5) supernatant is spray-dried, obtains pig placenta polypeptide.
Preferably, step (1) pig placenta 2~10min of homogeneous after blending；Step (2) water is pure water.
Preferably, before step (3) described mixing, the meat gruel liquid is ultrasonically treated 10~30min, is subsequently placed in subcritical
20~60min of resolution process in equipment.
Preferably, the power of the ultrasonic treatment is 200~700w.
Preferably, the temperature of resolution process is 110~150 DEG C in the subcritical equipment, and pressure is 0~6MPa.
Preferably, step (3) described complex enzyme in parts by mass, including 1~2 part of trypsase, 1~2 part of pawpaw
Protease and 2~3 parts of flavor protease.
Preferably, step (3) described flavor protease includes aminopeptidase and carboxypeptidase, the matter of the aminopeptidase and carboxypeptidase
Amount part is compared for 0.8~1.2:1.
Preferably, the total dose of step (3) described radiation sterilizing is 6~8kGy；The reaction temperature of the enzymatic hydrolysis is
36~50 DEG C, initial pH value is 6~7.5.
Preferably, the revolving speed of step (4) described centrifugation is 5000~12000r/min, and the aperture of filtering filter membrane is
0.4~0.5 μm.
Preferably, the outlet temperature of step (5) described spray drying is 60~70 DEG C.
The present invention provides a boar placenta polypeptide production methods, will first blend after the cleaning of pig placenta, obtain pig placenta meat
It is rotten；Then the pig placenta meat gruel is mixed with the water of 2~10 times of pig placenta meat gruel quality, obtains meat gruel liquid；Again by the meat
Rotten liquid is mixed with the complex enzyme of 0.5%~10% pig placenta meat gruel quality, obtains mixed liquor；It irradiates and kills after mixed liquor vacuum packaging
Bacterium, then enzyme digestion reaction 4~for 24 hours, obtains enzymolysis liquid；It will be filtered after enzymolysis liquid centrifugation again, obtain supernatant；Finally by institute
Supernatant spray drying is stated, pig placenta polypeptide is obtained.The present invention first cleans pig placenta, removes amount of grease, then using homogeneous
Method blends pig placenta sufficiently, increases enzymolysis efficiency；Enzymatic hydrolysis can effectively kill microorganism after vacuum irradiation sterilization, guarantee enzymatic hydrolysis
The breeding of microorganism is not present in the process；Enzymolysis process excludes air, carries out entirely reaction under anaerobic, inhibits coloured
Substance generates.The present invention is first extracted and is decomposed to pig placenta preferably by subcritical water, then with irradiation technique sterilization and disinsection,
Then it is digested, is finally spray-dried under vacuum conditions.Enzymolysis time under subcritical auxiliary is shorter, and decomposition is more thorough,
Yield is higher.Method reaction condition provided by the invention is mild, and high income, period are short, pollution-free, and the pig placenta polypeptide of acquisition can
Exploitation is functional beverage and health product, polypeptide products fishy smell is light, color just.
Specific embodiment
The present invention provides a boar placenta polypeptide production methods, include the following steps:
(1) it will be blended after the cleaning of pig placenta, obtain pig placenta meat gruel；
(2) the pig placenta meat gruel is mixed with the water of 2~10 times of pig placenta meat gruel quality, obtains meat gruel liquid；
(3) the meat gruel liquid is mixed with the complex enzyme of 0.5%~10% pig placenta meat gruel quality, obtains mixed liquor；It is mixed
Radiation sterilizing after liquid vacuum packaging is closed, then enzyme digestion reaction 4~for 24 hours, obtains enzymolysis liquid；
(4) it will be filtered after enzymolysis liquid centrifugation, obtain supernatant；
(5) supernatant is spray-dried, obtains pig placenta polypeptide.
The present invention will first blend after the cleaning of pig placenta.In the present invention, the pig placenta is preferably fresh pig placenta；Work as institute
When stating pig placenta to freeze pig placenta, the freezing pig placenta is preferably placed in 30~50 DEG C of warm water by the present invention to thaw, wait solve
It is cleaned again after the completion of freezing.The present invention is not particularly limited the specific source of the pig placenta, this field conventional commercial symbol
The pig placenta product of combined foodstuff safety standard.In the present invention, the temperature of the cleaning is preferably 30~50 DEG C, more preferably
It is 37 DEG C.Water is preferably periodically changed in the cleaning, and the time interval for changing water is preferably 8~12min/ times, more preferably
10min/ times.The total time of the cleaning is preferably 30~60min.The present invention first cleans pig placenta, can remove amount of grease,
Effectively remove bad smell.
After cleaning, the present invention blends pig placenta, obtains pig placenta meat gruel.The present invention is to the concrete operations side blended
Method is not particularly limited, this field routine operation.In the present invention, described to blend rear preferred progress homogeneous, the homogeneous
Revolving speed be preferably 1000~10000r/min, more preferably 5000r/min；In the present invention, the time of the homogeneous is preferred
For 2~10min, more preferably 5min.
After obtaining pig placenta meat gruel, the present invention mixes the pig placenta meat gruel with water, obtains meat gruel liquid.In the present invention
In, the quality of the water is preferably 2~10 times of pig placenta meat gruel quality, more preferably 6 times of pig placenta meat gruel quality.In the present invention
In, the water is preferably pure water.
The meat gruel liquid is preferably ultrasonically treated by the present invention.In the present invention, the power of the ultrasonic treatment is preferably 200
~700w, more preferably 600w；The time of the ultrasonic treatment is preferably 10~30min, more preferably 20min.
After ultrasonic treatment, meat gruel liquid is preferably placed in resolution process in subcritical equipment by the present invention.In the present invention, described
The temperature of resolution process is preferably 110~150 DEG C in subcritical equipment, more preferably 120~140 DEG C, more preferably 130 DEG C.
The pressure of resolution process is preferably 0~6MPa in the subcritical equipment, more preferably 1.5~4MPa, more preferably 3MPa.Institute
The time for stating resolution process in subcritical equipment is preferably 20~90min, more preferably 45min.The present invention is faced using the Asia of water
Boundary's state carries out resolution process to pig placenta meat gruel, can arrive most sugar and most proteolytic in pig placenta
In water.
After resolution process, the present invention mixes the meat gruel liquid after resolution process with complex enzyme.In the present invention, described compound
The dosage of enzyme is preferably the 0.5%~10% of the pig placenta meat gruel quality, and the 1% of the more preferably described pig placenta quality.This
Invention is hydrolyzed the albumen in decomposed solution using complex enzyme, and in the present invention, the complex enzyme in parts by mass, preferably wraps
Include 1~2 part of trypsase, 1~2 part of papain and 2~3 parts of flavor protease；It more preferably include 1 part of pancreas egg
White enzyme, 1 part of papain and 2 parts of flavor protease.In the present invention, the flavor protease preferably includes aminopeptidase
And carboxypeptidase, mass parts the ratio preferably 0.8~1.2:1, more preferably 1:1 of the aminopeptidase and carboxypeptidase.The present invention is to institute
The source for stating trypsase, papain, aminopeptidase and carboxypeptidase is not particularly limited, and this field conventional commercial product is equal
It can.
After obtaining mixed liquor, the present invention preferably adjusts the pH value of mixed liquor, and carries out vacuum packing to mixed liquor.It adjusts mixed
It is preferable to use sodium hydroxide reagents for the pH value of conjunction liquid.Mixed liquor pH value after adjusting is determining according to the demand of subsequent enzyme digestion reaction,
Preferably 6~7.5, more preferably 7.In the present invention, vacuum packing can make subsequent enzyme digestion reaction under vacuum conditions into
Row is conducive to the generation for inhibiting coloring matter in enzymolysis process.In the present invention, after vacuum packing, every bag of mixed liquor
Quality is preferably 1~5kg, more preferably 3kg.
After vacuum packaging, the present invention carries out radiation sterilizing to the mixed liquor after the vacuum packaging.In the present invention, described
Radiation sterilizing can be irradiated with single side, two-sided can also be irradiated.The total dose of the radiation sterilizing is preferably 6~8kGy, more
Preferably 7kGy.The radiation sterilizing can effectively kill microorganism, guarantee that microorganism is irreproducible in subsequent enzymolysis process.
Enzyme digestion reaction is carried out after mixed liquor radiation sterilizing, obtains enzymolysis liquid.In the present invention, the reaction temperature of the enzymatic hydrolysis
Preferably 36~50 DEG C, more preferably 37 DEG C.The initial pH value of the enzyme digestion reaction is preferably 6~7.5, and more preferably 7.It is described
The time of enzyme digestion reaction is preferably 4~for 24 hours, more preferably 8~12h.
After obtaining enzymolysis liquid, the enzymolysis liquid is centrifuged by the present invention.The revolving speed of the centrifugation is preferably 5000~12000r/
Min, more preferably 6000~7000r/min.It is filtered after centrifugation, the aperture of filtering filter membrane is preferably 0.4~0.5 μm,
More preferably 0.45 μm.
Supernatant is obtained after filtering, the supernatant is spray-dried by the present invention.In the present invention, the spray drying
Inlet temperature is 160-220 DEG C, more preferably 180-200 DEG C, outlet temperature is preferably 60~70 DEG C, spray drying
Afterwards to get arrive pig placenta polypeptide.
The present invention is first extracted and is decomposed to pig placenta using subcritical water, then with irradiation technique sterilization and disinsection, then
It is digested, is finally spray-dried under vacuum conditions.The production cycle of entire pig placenta polypeptide is 12~16h.Table after measured
Bright: method pig placenta resolution ratio provided by the invention is up to 95~98%.Pig placenta polypeptide powder 5.5 can be recycled in 100 parts of pig placentas
It is~6 parts, higher compared to conventional enzyme solution resolution ratio and the rate of recovery.In addition, method provided by the invention also have it is pollution-free
The characteristics of, the pig placenta polypeptide of acquisition can be developed into functional beverage and health product, polypeptide products fishy smell is light, color just, tool
There are good market prospects.
Technical solution provided by the invention is clearly and completely described below in conjunction with embodiment.Under it should be noted that
Embodiment cited by face is only section Example of the invention, instead of all the embodiments.Based on the embodiment of the present invention,
Every other embodiment obtained by those of ordinary skill in the art without making creative efforts, belongs to this hair
The range of bright protection.
Embodiment 1
It will be blended after the cleaning of 1kg fresh pig placenta, obtain pig placenta meat gruel.5kg water is added in meat gruel, stirs evenly, so
25g complex enzyme is added afterwards, obtains mixed liquor, the ratio of complex enzyme is trypsase: papain: flavor protease=1:1:
2.Mixed liquor is vacuum-packed into two bags, then single side radiation sterilizing, irradiation dose 6kGy, then 45 DEG C of enzyme digestion reaction 20h,
Obtain enzymolysis liquid.Spray drying obtains pig placenta polypeptide powder after finally enzymolysis liquid is filtered.
Embodiment 2
It will be blended after the cleaning of 1kg fresh pig placenta, obtain pig placenta meat gruel.3kg water is added in meat gruel, stirs evenly, so
10000r/min homogeneous 2min afterwards, obtains homogenizing fluid.Homogenizing fluid is put into subcritical equipment and decomposes 60min, undercritical conditions is
130 DEG C, 1Mpa.10g complex enzyme is added into the pig placental fluids after subcritical decomposition, obtains mixed liquor, complex enzyme ratio is pancreas
Protease: papain: flavor protease=2:1:1.Mixed liquor is vacuum-packed into two bags, then two-sided radiation sterilizing,
Irradiation accumulated dose is 6kGy, and then 37 DEG C of enzyme digestion reaction 8h, obtain enzymolysis liquid.Spray drying obtains after finally enzymolysis liquid is filtered
Pig placenta powder.
Embodiment 3
It will be blended after the cleaning of 1kg fresh pig placenta, obtain pig placenta meat gruel.10kg water is added in meat gruel, stirs evenly, so
5000r/min homogeneous 5min afterwards, obtains homogenizing fluid.Homogenizing fluid is put into subcritical equipment and decomposes 30min, undercritical conditions is
150 DEG C, 0.5Mpa.20g complex enzyme is added into the pig placental fluids after subcritical decomposition, obtains mixed liquor, complex enzyme ratio is
Trypsase: papain: flavor protease=2:1:2.Mixed liquor is vacuum-packed into 4 bags, then two-sided radiation sterilizing,
Irradiation accumulated dose is 6kGy, and then 50 DEG C of enzyme digestion reaction 4h, obtain enzymolysis liquid.Spray drying obtains after finally enzymolysis liquid is filtered
Pig placenta powder.
Embodiment 4
It will be blended after the cleaning of 1kg fresh pig placenta, obtain pig placenta meat gruel.4kg water is added in meat gruel, stirs evenly, so
8000r/min homogeneous 3min afterwards, obtains homogenizing fluid.Homogenizing fluid is put into subcritical equipment and decomposes 45min, undercritical conditions is
120 DEG C, 2Mpa.30g complex enzyme is added into the pig placental fluids after subcritical decomposition, obtains mixed liquor, complex enzyme ratio is pancreas
Protease: papain: flavor protease=2:2:2.Mixed liquor is vacuum-packed into 2 bags, then two-sided radiation sterilizing, spoke
It is 8kGy according to accumulated dose, then 37 DEG C of enzyme digestion reaction 12h, obtain enzymolysis liquid.Spray drying obtains after finally enzymolysis liquid is filtered
Pig placenta powder.
The analysis of physical and chemical property of pig placenta polypeptide produced by the invention the results are shown in Table 1~2
The basic composition of the powder of 1 pig placenta polypeptide of table
| Ingredient | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 |
| Protein content (%) | 78.28 | 80.33 | 80.51 | 82.12 |
| Polyoses content (%) | 8.23 | 8.01 | 8.25 | 8.05 |
| Moisture content (%) | 7.51 | 6.78 | 6.42 | 6.19 |
The molecular weight distribution of 2 pig placenta polypeptide of table
| Molecular weight distribution (Da) | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 |
| > 1000 | 8.31 | 5.8 | 4.17 | 5.65 |
| 500-1000 | 20.56 | 22.41 | 22.82 | 20.11 |
| 150-500 | 66.23 | 67.58 | 68.7 | 69.49 |
| < 150 | 4.9 | 4.21 | 4.31 | 4.75 |
Precritical method can be used in pre-treatment of the present invention keeps the albumen of pig placenta molten by controlling pressure and temperature
Solution comes out, can be to protein preliminary exposition since subcritical water has soda acid characteristic.It is killed again by vacuum packaging and irradiation
Bacterium carries out enzymatic hydrolysis in gnotobasis, prevents from turn ensuring enzymolysis efficiency while microbial contamination.Obtained polypeptide
Middle protein content is up to 78%-82%.The molecular weight of the pig placenta polypeptide of preparation is detected, the results showed that molecular weight master
It is distributed in 150-500Da.Show that pig placenta polypeptide is mainly the oligopeptides being made of 2-4 amino acid.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
1. a boar placenta polypeptide production method, which comprises the steps of:
(1) it will be blended after the cleaning of pig placenta, obtain pig placenta meat gruel；
(2) the pig placenta meat gruel is mixed with the water of 2~10 times of pig placenta meat gruel quality, obtains meat gruel liquid；
(3) the meat gruel liquid is mixed with the complex enzyme of 0.5%~10% pig placenta meat gruel quality, obtains mixed liquor；Mixed liquor
Radiation sterilizing after vacuum packaging, then enzyme digestion reaction 4~for 24 hours, obtains enzymolysis liquid；
(4) it will be filtered after enzymolysis liquid centrifugation, obtain supernatant；
(5) supernatant is spray-dried, obtains pig placenta polypeptide.
2. production method according to claim 1, which is characterized in that step (1) the pig placenta after blending homogeneous 2~
10min；Step (2) water is pure water.
3. production method according to claim 1, which is characterized in that before step (3) described mixing, the meat gruel liquid is surpassed
10~30min of sonication is subsequently placed in 20~90min of resolution process in subcritical equipment.
4. production method according to claim 3, which is characterized in that the power of the ultrasonic treatment is 200~700w.
5. production method according to claim 3, which is characterized in that the temperature of resolution process is in the subcritical equipment
110~150 DEG C, pressure is 0~6MPa.
6. production method according to claim 1, which is characterized in that step (3) described complex enzyme in parts by mass, including
1~2 part of trypsase, 1~2 part of papain and 2~3 parts of flavor protease.
7. production method according to claim 6, which is characterized in that step (3) described flavor protease includes aminopeptidase
And carboxypeptidase, the mass parts ratio of the aminopeptidase and carboxypeptidase are 0.8~1.2:1.
8. production method according to claim 1, which is characterized in that the total dose of step (3) described radiation sterilizing
For 6~8kGy；The reaction temperature of the enzymatic hydrolysis is 36~50 DEG C, and initial pH value is 6~7.5.
9. production method according to claim 1, which is characterized in that the revolving speed of step (4) described centrifugation be 5000~
The aperture of 12000r/min, the filtering filter membrane are 0.4~0.5 μm.
10. production method according to claim 1, which is characterized in that the outlet temperature of step (5) described spray drying is
60~70 DEG C.
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|---|---|---|---|
| CN201910710845.2A CN110331180B (en) | 2019-08-02 | 2019-08-02 | Method for producing pig placenta polypeptide |
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| CN110331180B CN110331180B (en) | 2021-04-16 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112205518A (en) * | 2020-10-21 | 2021-01-12 | 清远八蚨生物有限公司 | Enzymolysis method for postpartum placenta of sow |
| CN114767717A (en) * | 2022-05-20 | 2022-07-22 | 华中农业大学 | Preparation method and application of pig placenta extract |
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| CN105420325A (en) * | 2015-12-28 | 2016-03-23 | 贵州泰邦生物制品有限公司 | Placenta polypeptide preparation method |
| CN107556364A (en) * | 2017-09-30 | 2018-01-09 | 厦门市百肽生物科技有限公司 | Subcritical water assistance enzymolysis extracts the method and product of abalone proteins peptide |
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| KR20150067954A (en) * | 2013-12-11 | 2015-06-19 | 건국대학교 산학협력단 | Method for preparing placenta of low-molecular weight by subcritical water and high pressure enzyme process |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112205518A (en) * | 2020-10-21 | 2021-01-12 | 清远八蚨生物有限公司 | Enzymolysis method for postpartum placenta of sow |
| CN114767717A (en) * | 2022-05-20 | 2022-07-22 | 华中农业大学 | Preparation method and application of pig placenta extract |
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