CN110317715A - A kind of mixed fermentation device for laboratory liquid state fermentation - Google Patents
A kind of mixed fermentation device for laboratory liquid state fermentation Download PDFInfo
- Publication number
- CN110317715A CN110317715A CN201910756709.7A CN201910756709A CN110317715A CN 110317715 A CN110317715 A CN 110317715A CN 201910756709 A CN201910756709 A CN 201910756709A CN 110317715 A CN110317715 A CN 110317715A
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- fermentation
- liquid state
- tank
- screw thread
- laboratory
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- 238000000855 fermentation Methods 0.000 title claims abstract description 76
- 230000004151 fermentation Effects 0.000 title claims abstract description 76
- 239000007788 liquid Substances 0.000 title claims abstract description 46
- 239000011521 glass Substances 0.000 claims abstract description 14
- 238000004891 communication Methods 0.000 claims abstract description 10
- 239000000463 material Substances 0.000 claims description 8
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- 241000894006 Bacteria Species 0.000 abstract description 8
- 238000011081 inoculation Methods 0.000 abstract description 7
- 238000009434 installation Methods 0.000 abstract description 6
- 210000001082 somatic cell Anatomy 0.000 abstract description 4
- 235000014101 wine Nutrition 0.000 abstract description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 239000002131 composite material Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000005189 flocculation Methods 0.000 description 2
- 230000016615 flocculation Effects 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 230000035479 physiological effects, processes and functions Effects 0.000 description 2
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003851 biochemical process Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
Abstract
The invention discloses a kind of mixed fermentation devices for laboratory liquid state fermentation, belong to wine brewing engineering field.The present invention solves the problems, such as that two kinds of bacterium of combined inoculation cannot be separated and come into full contact with fermentation liquid by laboratory ordinary liquid installation for fermenting.A kind of mixed fermentation device for laboratory liquid state fermentation, include tank body (1), it is characterized by: tank body (1) setting tank mouth (7) and side port (6), two tank bodies (1) are connected to by tank mouth (7), external screw thread is arranged in tank mouth (7), junction is tightly connected by being provided with the attachment device (2) of the matched internal screw thread of tank mouth (7) external screw thread, and bed course (3) are arranged at tank mouth (7).The present invention passes through increase positive/negative thread communication apparatus, the independent bed course and filter paper of central zone mesh, it can ensure that fermentation liquid be inoculated with somatic cells while coming into full contact with separate in two glass jar bodies, it solves the problems, such as that ordinary device cannot guarantee that thallus separates while fermentation liquid contacts, thallus and fermentation is influenced to provide suitable scientific research device for laboratory research combined inoculation fermentation.
Description
Technical field
The invention discloses a kind of mixed fermentation devices for laboratory liquid state fermentation, belong to wine brewing engineering field.
Background technique
Combined inoculation fermentation all kinds of fermented wines brewing in receives more and more attention, different strains simultaneously be inoculated with or
Sequence is inoculated in liquid state fermentation liquid, to realize different fermentation physiology biochemical processes, improves the flavor complexity of brewed wine.It is existing
Having laboratory liquid installation for fermenting is mostly common mixed fermentation device, i.e., it is empty to be present in the same fermentation for inoculation thallus and fermentation liquid
In, it is especially unable to satisfy when being inoculated with using composite bacteria and separates different bacterium under the premise of mixed fermentation liquid
Kind.May be contacted by cell research shows that composite bacteria is when in use, between different somatic cells occur mutually to kill or
The case where inhibition, its fermentation physiology biochemical action can not normally be played by causing to be killed or be suppressed bacterium cell.
Summary of the invention
In order to solve the above technical problems, the present invention provides a kind of mixed fermentation device for laboratory liquid state fermentation,
It can ensure that fermentation liquid be inoculated with somatic cells while coming into full contact with separate in two glass jar bodies, solving ordinary device cannot
Guarantee the problem of thallus separates while fermentation liquid contact, thallus and fermentation are influenced for laboratory research combined inoculation fermentation
Suitable scientific research device is provided.
The technical solution adopted by the present invention to solve the technical problems is: a kind of mixing hair for laboratory liquid state fermentation
Ferment device includes tank body, and the tank body setting tank mouth and side port, two tank bodies are connected to by tank mouth, and external screw thread is arranged in tank mouth,
Junction is tightly connected by being provided with the attachment device of the matched internal screw thread of tank mouth external screw thread, and bed course is arranged at tank mouth.
Bracket is set under the tank body.
The tank body material is glass, in order to monitor concrete condition (blistering, thallus flocculation, hair of fermentation liquid in real time
Zymotic fluid height, fermentation liquid color etc.).
Side port lid is arranged in the side port, and external screw thread is arranged in side port, and matched internal screw thread is arranged in side port lid.
Tank body, communication apparatus, bed course and the side port lid is made of the material for being resistant to the requirement of high pressure steam sterilization.I.e.
Material does not deform through 121 DEG C of 20min and damaged condition.The installation for fermenting can pass through high steam before scientific experiment
The mode of sterilizing directly sterilizes, and guarantees the germ-free condition of installation for fermenting.Communication apparatus material is plastics, and bed course is stainless steel or silicon
Glue.
The bed course central web structure is filter paper, and filter paper aperture is 0.45 μm or 0.22 μm.The filter paper aperture is
Allowing fermentation liquid to pass through at 0.45 μm does not allow yeast and the bigger microorganism of diameter to pass through, when the filter paper aperture is 0.22 μm
Allowing fermentation liquid to pass through does not allow bacterium, yeast and the bigger microorganism of diameter to pass through.
Beneficial effects of the present invention: a kind of mixed fermentation device for laboratory liquid state fermentation provided by the invention leads to
Cross the independent bed course and filter paper for increasing positive/negative thread communication apparatus, central zone mesh, it can be ensured that fermentation liquid in two glass jar bodies
Be inoculated with somatic cells separate while coming into full contact with, and thallus cannot be guaranteed while fermentation liquid contacts by solving ordinary device
The problem of separation, influences to provide suitable scientific research device for laboratory research combined inoculation fermentation on thallus and fermentation.
Detailed description of the invention
Fig. 1 is a kind of glass tank body schematic diagram of mixed fermentation device for laboratory liquid state fermentation of embodiment；
Fig. 2 is a kind of communication apparatus schematic diagram of mixed fermentation device for laboratory liquid state fermentation of embodiment；
Fig. 3 is a kind of independent bed course schematic diagram of mixed fermentation device for laboratory liquid state fermentation of embodiment；
Fig. 4 is a kind of overall schematic of mixed fermentation device for laboratory liquid state fermentation of embodiment.
Specific embodiment
Of the invention for ease of understanding, below in conjunction with drawings and examples, the present invention will be described in detail, the embodiment
For explaining only the invention, protection scope of the present invention is not constituted and is limited, it is all using equivalent replacement or the side of equivalency transform
The technical solution that formula obtains is fallen within the scope of protection of the present invention.
Embodiment
As shown in Figures 1 to 4,
A kind of mixed fermentation device for laboratory liquid state fermentation, includes tank body 1, and tank mouth 7 and side port is arranged in the tank body 1
6, two tank bodies 1 are connected to by tank mouth 7, and external screw thread is arranged in tank mouth 7, and junction is matched with 7 external screw thread of tank mouth by being provided with
The attachment device 2 of internal screw thread is tightly connected, and bed course 3 is arranged at tank mouth 7, be used to support filter paper with ensure that glass tank body connects tight
Close property；The lower setting bracket 4 of the tank body 1；1 material of tank body is glass；Side port lid 5, side port 6 is arranged in the side port 6
External screw thread is set, and matched internal screw thread is arranged in side port lid 5.Tank body 1, communication apparatus 2, bed course 3 and the side port lid 5 be
The material for being resistant to the requirement of high pressure steam sterilization is made.The 3 central web structure of bed course is filter paper, and the filter paper aperture is
Allowing fermentation liquid to pass through at 0.45 μm does not allow yeast and the bigger microorganism of diameter to pass through, when the filter paper aperture is 0.22 μm
Allowing fermentation liquid to pass through does not allow bacterium, yeast and the bigger microorganism of diameter to pass through.
A kind of mixed fermentation device for laboratory liquid state fermentation of the present embodiment, two glass tank bodies of setting can
For containing same fermentation liquid but being inoculated with different strains respectively；The independent bed course of the central zone mesh of setting, is used to support
Filter paper adds ordinary filter paper that can play and separates different strain but do not separate hair with the compactness for ensuring that glass tank body connects, the bed course
The effect of zymotic fluid；Setting with right-and-left threaded communication apparatus, for guaranteeing that two glass tank bodies are screwed in and are completely embedded；Setting
Glass tank body, communication apparatus, bed course and thread cap can tolerate high pressure steam sterilization, the installation for fermenting can before scientific experiment
It is directly sterilized by way of high pressure steam sterilization, guarantees the germ-free condition of installation for fermenting；The glass tank body of setting is convenient for real-time
The concrete condition (blistering, thallus flocculation, fermentation liquid height, fermentation liquid color etc.) of monitor fermentation liquid.
A kind of mixed fermentation device for laboratory liquid state fermentation of the present embodiment, by increase threaded communication device,
The independent bed course and filter paper of central zone mesh, it can be ensured that fermentation liquid be inoculated with bacterium while coming into full contact in two glass jar bodies
Body cell separates, and solves the problems, such as that ordinary device cannot guarantee that thallus separates while fermentation liquid contacts, is laboratory research
Combined inoculation fermentation influences to provide suitable scientific research device on thallus and fermentation.
Claims (6)
1. a kind of mixed fermentation device for laboratory liquid state fermentation includes tank body (1), it is characterised in that:
Tank body (1) setting tank mouth (7) and side port (6), two tank bodies (1) are connected to by tank mouth (7), tank mouth (7) setting
External screw thread, junction are tightly connected by being provided with the attachment device (2) of the matched internal screw thread of tank mouth (7) external screw thread, tank mouth
(7) bed course (3) are set at.
2. a kind of mixed fermentation device for laboratory liquid state fermentation according to claim 1, it is characterised in that: described
Bracket (4) are set under tank body (1).
3. a kind of mixed fermentation device for laboratory liquid state fermentation according to claim 1, it is characterised in that: described
Tank body (1) material be glass.
4. a kind of mixed fermentation device for laboratory liquid state fermentation according to claim 1, it is characterised in that: described
Side port (6) side port lid (5) are set, external screw thread is arranged in side port (6), and matched internal screw thread is arranged in side port lid (5).
5. a kind of mixed fermentation device for laboratory liquid state fermentation according to claim 4, it is characterised in that: described
Tank body (1), communication apparatus (2), bed course (3) and side port lid (5) be made of the material of requirement for being resistant to high pressure steam sterilization.
6. a kind of mixed fermentation device for laboratory liquid state fermentation according to claim 1, it is characterised in that: described
Bed course (3) central web structure be filter paper, filter paper aperture be 0.45 μm or 0.22 μm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910756709.7A CN110317715A (en) | 2019-08-16 | 2019-08-16 | A kind of mixed fermentation device for laboratory liquid state fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910756709.7A CN110317715A (en) | 2019-08-16 | 2019-08-16 | A kind of mixed fermentation device for laboratory liquid state fermentation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110317715A true CN110317715A (en) | 2019-10-11 |
Family
ID=68126168
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910756709.7A Pending CN110317715A (en) | 2019-08-16 | 2019-08-16 | A kind of mixed fermentation device for laboratory liquid state fermentation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110317715A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111543621A (en) * | 2020-05-09 | 2020-08-18 | 黑龙江大学 | Perilla frutescens chili sauce with health-care function and preparation method thereof |
| CN114106954A (en) * | 2021-12-17 | 2022-03-01 | 广西壮族自治区农业科学院 | Device suitable for mixed fermentation of multiple microorganism |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102203605A (en) * | 2008-08-27 | 2011-09-28 | 生命技术公司 | Apparatus for and method of processing biological samples |
| CN103937662A (en) * | 2012-12-31 | 2014-07-23 | 江苏嘉语生物医药技术有限公司 | Microbe separating and culturing device and method |
| CN206204299U (en) * | 2016-10-19 | 2017-05-31 | 河南师范大学 | A kind of microorganism allelopathy culture apparatus |
| CN107460117A (en) * | 2017-09-04 | 2017-12-12 | 清华大学 | A kind of experimental provision and method for resistant gene Horizontal Gene Transfer research |
| CN208008809U (en) * | 2017-12-29 | 2018-10-26 | 山东省医疗器械产品质量检验中心 | 1.2 μm of normal pore size liquid medicine filter Candida albicans rejection tests device |
| CN210367653U (en) * | 2019-08-16 | 2020-04-21 | 贵州大学 | Mixed fermentation device for laboratory liquid fermentation |
-
2019
- 2019-08-16 CN CN201910756709.7A patent/CN110317715A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102203605A (en) * | 2008-08-27 | 2011-09-28 | 生命技术公司 | Apparatus for and method of processing biological samples |
| CN103937662A (en) * | 2012-12-31 | 2014-07-23 | 江苏嘉语生物医药技术有限公司 | Microbe separating and culturing device and method |
| CN206204299U (en) * | 2016-10-19 | 2017-05-31 | 河南师范大学 | A kind of microorganism allelopathy culture apparatus |
| CN107460117A (en) * | 2017-09-04 | 2017-12-12 | 清华大学 | A kind of experimental provision and method for resistant gene Horizontal Gene Transfer research |
| CN208008809U (en) * | 2017-12-29 | 2018-10-26 | 山东省医疗器械产品质量检验中心 | 1.2 μm of normal pore size liquid medicine filter Candida albicans rejection tests device |
| CN210367653U (en) * | 2019-08-16 | 2020-04-21 | 贵州大学 | Mixed fermentation device for laboratory liquid fermentation |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111543621A (en) * | 2020-05-09 | 2020-08-18 | 黑龙江大学 | Perilla frutescens chili sauce with health-care function and preparation method thereof |
| CN114106954A (en) * | 2021-12-17 | 2022-03-01 | 广西壮族自治区农业科学院 | Device suitable for mixed fermentation of multiple microorganism |
| CN114106954B (en) * | 2021-12-17 | 2023-11-14 | 广西壮族自治区农业科学院 | Device suitable for mixed fermentation of multiple microorganism |
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