CN110302134A - One kind body eye cream preparation of excretion containing human umbilical cord mesenchymal stem cells and preparation method thereof - Google Patents
One kind body eye cream preparation of excretion containing human umbilical cord mesenchymal stem cells and preparation method thereof Download PDFInfo
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- CN110302134A CN110302134A CN201910667967.8A CN201910667967A CN110302134A CN 110302134 A CN110302134 A CN 110302134A CN 201910667967 A CN201910667967 A CN 201910667967A CN 110302134 A CN110302134 A CN 110302134A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/005—Preparations for sensitive skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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Abstract
The present invention relates to cell biological engineering technology and medical cosmetology fields, disclose one kind body eye cream preparation of excretion containing human umbilical cord mesenchymal stem cells and preparation method.It is tested by a large amount of safeties and human efficiency, it is found that the eye cream preparation safety is non-stimulated, dispelling the dry line microgroove of eye, desalinate true property line and improve significant effect in terms of skin hydration degree.
Description
Technical field
It is specifically a kind of dry thin containing human umbilical cord mesenchymal the present invention relates to cell biological engineering technology and medical cosmetology field
It is extracellular to secrete body eye cream preparation and preparation method thereof.
Background technique
Excretion body is the film property lipid vesicles of 30~150nm of diameter for being secreted and being formed by cell a kind of, excretion somatocyst bubble
Film has lipid bilayer structure, is rich in multiple proteins, lipid and nucleic acid such as mRNA, miRNA etc. in vesica.Cell excretion
Proliferation, migration, the apoptosis etc. that body passes through the controllable target cell of transhipment of these three contents.
Mescenchymal stem cell is a kind of cell with self-renewing and multi-lineage potential, especially human umbilical cord mesenchymal
Stem cell has extensive clinical value and application prospect.Studies have shown that human umbilical cord mesenchymal stem cells excretion body can promote
Skin fibroblasts proliferation, accelerates wound healing and revascularization, and can accelerate collagen and be formed.
The present invention body of excretion containing human umbilical cord mesenchymal stem cells, and it is formulated alcohol-free, non-pigment, without essence, preservative free,
The bacteriostasis of formula derives from bright string coccus/radish root tunning filtrate, has broad-spectrum antibacterial, and be pure natural fermentation
Source, while the ingredient also has an excellent moisturizing, anti-acne, repair ability, can increase for eye cream of the present invention certain moisturizing and
Repair effect.Consider the fragility of eye part skin, each component in stringent screening formula, if 1,3-PD is natural corn
Source；Beta glucan, natural origin, without chemical modification, and (Isosorbide-5-Nitrae-and 1,3- link are without branch for its unique molecular structure
The straight chain oat D-glucose polymer of chain) make its good water solubility, it is easier to assist other activated feedstock osmotic absorptions；It compares
Compared with the eye creams such as existing spirituosity and preservative on the market, the present invention is more safe and effective, and excretion body extracting solution is combined to enhance
Its moisturizing, anti-wrinkle ability, being compared to common umbilical cord mesenchymal stem cells factor freeze-dried powder etc. has stronger activity and guarantor
Wet anti-wrinkle ability.
Summary of the invention
The purpose of the present invention is to provide a kind of body eye cream preparation of excretion containing human umbilical cord mesenchymal stem cells and its preparation sides
Method, to solve the problems mentioned in the above background technology.
To achieve the above object, the invention provides the following technical scheme:
A kind of body eye cream preparation of excretion containing human umbilical cord mesenchymal stem cells, including human umbilical cord mesenchymal stem cells excretion body with
Matrix, the matrix include solvent, moisturizer, thickener, chelating agent, emollient, emulsifier, skin conditioning agent, assistant for emulsifying agent,
Emulsion stabilizer and pH adjusting agent；The solvent is water.
As a further solution of the present invention: the human umbilical cord mesenchymal stem cells excretion body is antioxidant；
The moisturizer includes 1,3-PD, glycerin polyether -26, Sodium Hyaluronate and bright string coccus/radish root fermentation
Product filtrate；
The thickener includes xanthan gum and acrylic acid (ester) class/C10-30 alkanol propionic ester cross-linked polymer；
The chelating agent is EDETATE SODIUM；
The emollient includes PEG-75 sher butter glyceride type, sweet three ester of caprylic/capric, hydrolecithin and fertility
Phenol acetic acid esters；
The emulsifier is -6 distearate of polyglycereol；
The skin conditioning agent includes phytosterol, bisabolol and beta glucan；
The assistant for emulsifying agent is stearine；
The emulsion stabilizer is cetostearyl alcohol；
The pH adjusting agent is triethanolamine.
As a further solution of the present invention, by being made as follows:
As the present invention, further scheme, the body of excretion containing the human umbilical cord mesenchymal stem cells eye cream preparation are water-setting
Agent or lotion.
As the present invention, further scheme, the human umbilical cord mesenchymal stem cells excretion body are dry for human umbilical cord mesenchymal
Cell excretion body suspension.
The preparation method of the body of excretion containing the human umbilical cord mesenchymal stem cells eye cream preparation, steps are as follows:
1) raw material is divided into A group, B group, C group, D group, E group and F group, A group includes water, 1,3-PD, glycerin polyether-
26, xanthan gum, acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross-linked polymer and EDETATE SODIUM, B group include PEG-75 ox
Oleoresin glyceride type, -6 distearate of polyglycereol, caprylic/capric triglyceride, phytosterol, stearine,
Cetostearyl alcohol, hydrolecithin and tocopherol acetate, C group are bisabolol, and D group is triethanolamine, and E group is hyaluronic acid
Sodium, F group include beta glucan, bright string coccus/radish root tunning filtrate and human umbilical cord mesenchymal stem cells excretion body, will be saturating
Bright matter acid sodium is used water-swellable in advance；
2) after acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross-linked polymer and xanthan gum butanediol being dispersed,
Water is added and A group surplus material is heated to 75~80 DEG C, stirs evenly and is kept for 10 minutes；
3) B group material is heated to 80~85 DEG C, stirs evenly and keeps after ten minutes, it is uniform that C group material stirring being added；
4) step 2) products therefrom is slowly added into step 1) products therefrom, it is anti-stirs evenly progress emulsifying
It answers, it is uniform that D group material stirring is added；
5) E group material is added when being cooled to 45 DEG C, stirs evenly；
6) F group material is added when being cooled to 38 DEG C, is stirred to uniform；
7) 35 DEG C of detections are cooled to, discharging is stood, and the inspection of semifinished product is filling, warehousing finished products.
As further scheme of the invention: in step 6), when F group material is added, beta glucan, bright string coccus/trailing plants
Foretelling root tunning filtrate and human umbilical cord mesenchymal stem cells excretion body will sequentially add, and stir after the addition of each raw material equal
It is even that a kind of material can be added down.
Compared with prior art, the beneficial effects of the present invention are:
It is tested by a large amount of human efficiencies, it is found that the eye cream preparation is dispelling eye wrinkle-removing, in terms of improving skin hydration degree
It is effective, the dry line microgroove of eye especially is being dispelled, is desalinating significant effect in terms of true property line.38 subject's visual sense eyes
Portion's skin moisture content obtains instant promotion, and skin moisturizing shows that this product is aobvious to the promotion effect of the instant moisture content of skin
It writes；More than the instant elasticity that 80% subject thinks the promotion skin that this product can be different degrees of；It is anti-more than 70% subject
The wrinkle for presenting oneself makes moderate progress, and subject has obvious dry line microgroove and deeper true property line, eye before nursing in terms of anti-wrinkle
Skin is more obscure, and after eye-care, the dry line microgroove of eye disappears, and skin brightness is promoted；Depth, the length, width of true property line
Degree significantly reduces.Illustrate that this product can effectively dispel the dry line microgroove of eye, desalinates true property line.
Detailed description of the invention
Fig. 1 is the flow chart of preparation method of the present invention.
Fig. 2 is animal eye stimulation test picture in the embodiment of the present invention.Experimental animal is new zealand white rabbit, totally 8,
Raising adapts to 7d in laboratory conditions, and the eyes to test rabbit interior for 24 hours before on-test carry out fluorescein sodium inspection, nothing
It damages (Fig. 2A)；It instills after tested material at left eye (Fig. 2 B) and is compared after 72h with right eye (Fig. 2 C), eye is not damaged；To test rabbit
Eyes carry out fluorescein sodium and check that test paper does not change colour, it was demonstrated that after left eye administration not damaged (Fig. 2 D).
Fig. 3 is safety and the efficacy assessments figure of the embodiment of the present invention.Before carrying out eye-care to 38 volunteers, nursing
Afterwards, eye cream (by test product) of the present invention is used 28 days altogether using data acquisition is carried out after one week, two weeks, three weeks, four weeks.That is timeliness
Fruit: the data measured using before eye-care and the data measured in nursing latter hour carry out average value as immediate effect data
Processing, data improve degree before and after calculating volunteer's eye-care, to obtain improvement number ratio and not improve number ratio
(Fig. 3 A)；Long Shi Xiaoguo: made with the initial data before eye-care and by test product using latter week, two weeks, three weeks, four weekly datas
Average value processing is carried out for long-term effect data, every volunteer is calculated using the improvement degree after by test product, changes to obtain
Philanthropist's number ratio and do not improve number ratio (Fig. 3 B).
Fig. 4 is the effect contrast figure of the embodiment of the present invention.Volunteer's example 1 using (Fig. 4 A) before excretion body eye cream with use four
(Fig. 4 B) Contrast on effect after week, volunteer's example 2 is using (Fig. 4 C) before excretion body eye cream and uses (Fig. 4 D) Contrast on effect after four weeks.
Specific embodiment
The technical solution of the patent is explained in further detail With reference to embodiment.
The collection of embodiment 1, human umbilical cord mesenchymal stem cells supernatant
1. the culture of human umbilical cord mesenchymal stem cells:
The umbilical cord tissue for acquiring mature healthy babies is placed in the aseptic bottle equipped with PBS (mycillin containing 100U/mL)
In, processing in 4h.The vein and artery for removing umbilical cord, strip magnificent Tong Shi glue, are cut to 2mm3Size tissue block, in culture bottle
In it is evenly laid out, add a small amount of complete medium to keep wet, culture medium is that DMEM/F12 adds 15%FBS, is supplemented afterwards for 24 hours enough
Culture medium changes the liquid once for every 3 days later, and cell is long to pass on to after merging, and culture medium is changed to DMEM/F12 and adds 10% after passage
FBS。
2. the extraction of excretion body:
Excretion body is extracted with the human umbilical cord mesenchymal stem cells supernatant in the 3rd~5 generation, it is long to 80% or so fusion to cell
After degree, it is changed to serum free medium, after continuing culture for 24 hours, collects culture supernatant.
(1), by 4 DEG C of culture supernatant, 300g, it is centrifuged 10min, retains supernatant；
(2), 4 DEG C of supernatant, 2000g are taken, 10min is centrifuged, retains supernatant；
(3), 4 DEG C of supernatant, 10000g are taken, 30min is centrifuged, retains supernatant；
(4), 4 DEG C of supernatant, 100000g after being centrifuged, it is centrifuged 70min, abandons supernatant, precipitating is collected, will be collected with PBS
The precipitating centrifuge washing arrived one time；
(5), the excretion body after centrifuge washing is resuspended using physiological saline.
The component and preparation process of embodiment 2, human umbilical cord mesenchymal stem cells excretion body eye cream
1. a kind of body eye cream preparation of excretion containing human umbilical cord mesenchymal stem cells, including human umbilical cord mesenchymal stem cells excretion body
And matrix, the matrix include solvent, moisturizer, thickener, chelating agent, emollient, emulsifier, skin conditioning agent, help emulsification
Agent, emulsion stabilizer, pH adjusting agent；The solvent is water.
Wherein, the human umbilical cord mesenchymal stem cells excretion body is antioxidant；
The moisturizer includes 1,3-PD, glycerin polyether -26, Sodium Hyaluronate and bright string coccus/radish root fermentation
Product filtrate；
The thickener includes xanthan gum and acrylic acid (ester) class/C10-30 alkanol propionic ester cross-linked polymer；
The chelating agent is EDETATE SODIUM；
The emollient includes PEG-75 sher butter glyceride type, sweet three ester of caprylic/capric, hydrolecithin and fertility
Phenol acetic acid esters；
The emulsifier is -6 distearate of polyglycereol；
The skin conditioning agent includes phytosterol, bisabolol and beta glucan；
The assistant for emulsifying agent is stearine；
The emulsion stabilizer is cetostearyl alcohol；
The pH adjusting agent is triethanolamine.
Specifically, by weight percentage, the content of the human umbilical cord mesenchymal stem cells excretion body is 0.0005%, 1,3-
The content of propylene glycol is 2.0%, and the content of glycerin polyether -26 is 1.0%, and the content of xanthan gum is 0.05%, acrylic acid (ester)
Class/C10-30 alkylol acrylamide acid esters cross-linked polymer content is 0.05%, and the content of EDETATE SODIUM is 0.03%, PEG-75 ox
The content of oleoresin glyceride type is 2.0%, and the content of -6 distearate of polyglycereol is 1.5%, and stearine contains
Amount is 0.4%, and the content of phytosterol is 0.5%, and the content of bisabolol is 0.05%, and the content of beta glucan is
0.5%, the content of cetostearyl alcohol is 0.5%, and the content of triethanolamine is 0.05%, and the content of Sodium Hyaluronate is
0.01%, bright string coccus/radish root tunning filtrate content is 1.0%, and the content of sweet three ester of caprylic/capric is 2.5%,
The content of hydrolecithin is 0.001%, and the content of tocopherol acetate is 0.2%, and surplus is water.
Specifically, the body of excretion containing the human umbilical cord mesenchymal stem cells eye cream preparation is water-setting agent or lotion.
Specifically, the human umbilical cord mesenchymal stem cells excretion body is human umbilical cord mesenchymal stem cells excretion body suspension.
The preparation method of the body of excretion containing the human umbilical cord mesenchymal stem cells eye cream preparation, steps are as follows:
1) raw material is divided into A group, B group, C group, D group, E group and F group, A group includes water, 1,3-PD, glycerin polyether-
26, xanthan gum, acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross-linked polymer and EDETATE SODIUM, B group include PEG-75 ox
Oleoresin glyceride type, -6 distearate of polyglycereol, caprylic/capric triglyceride, phytosterol, stearine,
Cetostearyl alcohol, hydrolecithin and tocopherol acetate, C group are bisabolol, and D group is triethanolamine, and E group is hyaluronic acid
Sodium, F group include beta glucan, bright string coccus/radish root tunning filtrate and human umbilical cord mesenchymal stem cells excretion body, will be saturating
Bright matter acid sodium is used water-swellable in advance；
2) after acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross-linked polymer and xanthan gum butanediol being dispersed,
Water is added and A group surplus material is heated to 75~80 DEG C, stirs evenly and is kept for 10 minutes；
3) B group material is heated to 80~85 DEG C, stirs evenly and keeps after ten minutes, it is uniform that C group material stirring being added；
4) step 2) products therefrom is added slowly in step 1) products therefrom, it is anti-stirs evenly progress emulsifying
It answers, it is uniform that D group material stirring is added；
5) E group material is added when being cooled to 45 DEG C, stirs evenly；
6) F group material is added when being cooled to 38 DEG C, is stirred to uniform；
7) 35 DEG C of detections are cooled to, discharging is stood, and the inspection of semifinished product is filling, warehousing finished products.
Wherein, in step 6), when F group material is added, beta glucan, bright string coccus/radish root tunning filtrate and people
Umbilical cord mesenchymal stem cells excretion body will sequentially add, and each raw material be added after stir evenly a kind of object can be added down
Material.
2. a kind of body eye cream preparation of excretion containing human umbilical cord mesenchymal stem cells, including human umbilical cord mesenchymal stem cells excretion body
And matrix, the matrix include solvent, moisturizer, thickener, chelating agent, emollient, emulsifier, skin conditioning agent, help emulsification
Agent, emulsion stabilizer, pH adjusting agent；The solvent is water.
Wherein, the human umbilical cord mesenchymal stem cells excretion body is antioxidant；
The moisturizer includes 1,3-PD, glycerin polyether -26, Sodium Hyaluronate and bright string coccus/radish root fermentation
Product filtrate；
The thickener includes xanthan gum and acrylic acid (ester) class/C10-30 alkanol propionic ester cross-linked polymer；
The chelating agent is EDETATE SODIUM；
The emollient includes PEG-75 sher butter glyceride type, sweet three ester of caprylic/capric, hydrolecithin and fertility
Phenol acetic acid esters；
The emulsifier is -6 distearate of polyglycereol；
The skin conditioning agent includes phytosterol, bisabolol and beta glucan；
The assistant for emulsifying agent is stearine；
The emulsion stabilizer is cetostearyl alcohol；
The pH adjusting agent is triethanolamine.
Specifically, by weight percentage, the content of the human umbilical cord mesenchymal stem cells excretion body is 0.003%, 1,3-
The content of propylene glycol is 3.0%, and the content of glycerin polyether -26 is 2.0%, and the content of xanthan gum is 0.07%, acrylic acid (ester)
Class/C10-30 alkylol acrylamide acid esters cross-linked polymer content is 0.08%, and the content of EDETATE SODIUM is 0.04%, PEG-75 ox
The content of oleoresin glyceride type is 2.5%, and the content of -6 distearate of polyglycereol is 2.0%, and stearine contains
Amount is 0.6%, and the content of phytosterol is 0.7%, and the content of bisabolol is 0.1%, and the content of beta glucan is
0.7%, the content of cetostearyl alcohol is 0.9%, and the content of triethanolamine is 0.08%, and the content of Sodium Hyaluronate is
0.05%, bright string coccus/radish root tunning filtrate content is 2.0%, and the content of sweet three ester of caprylic/capric is 3.0%,
The content of hydrolecithin is 0.02%, and the content of tocopherol acetate is 0.4%, and surplus is water.
Specifically, the body of excretion containing the human umbilical cord mesenchymal stem cells eye cream preparation is water-setting agent or lotion.
Specifically, the human umbilical cord mesenchymal stem cells excretion body is human umbilical cord mesenchymal stem cells excretion body suspension.
The preparation method of the body of excretion containing the human umbilical cord mesenchymal stem cells eye cream preparation, steps are as follows:
1) raw material is divided into A group, B group, C group, D group, E group and F group, A group includes water, 1,3-PD, glycerin polyether-
26, xanthan gum, acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross-linked polymer and EDETATE SODIUM, B group include PEG-75 ox
Oleoresin glyceride type, -6 distearate of polyglycereol, caprylic/capric triglyceride, phytosterol, stearine,
Cetostearyl alcohol, hydrolecithin and tocopherol acetate, C group are bisabolol, and D group is triethanolamine, and E group is hyaluronic acid
Sodium, F group include beta glucan, bright string coccus/radish root tunning filtrate and human umbilical cord mesenchymal stem cells excretion body, will be saturating
Bright matter acid sodium is used water-swellable in advance；
2) after acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross-linked polymer and xanthan gum butanediol being dispersed,
Water is added and A group surplus material is heated to 75~80 DEG C, stirs evenly and is kept for 10 minutes；
3) B group material is heated to 80~85 DEG C, stirs evenly and keeps after ten minutes, it is uniform that C group material stirring being added；
4) step 2) products therefrom is added slowly in step 1) products therefrom, it is anti-stirs evenly progress emulsifying
It answers, it is uniform that D group material stirring is added；
5) E group material is added when being cooled to 45 DEG C, stirs evenly；
6) F group material is added when being cooled to 38 DEG C, is stirred to uniform；
7) 35 DEG C of detections are cooled to, discharging is stood, and the inspection of semifinished product is filling, warehousing finished products.
Wherein, in step 6), when F group material is added, beta glucan, bright string coccus/radish root tunning filtrate and people
Umbilical cord mesenchymal stem cells excretion body will sequentially add, and each raw material be added after stir evenly a kind of object can be added down
Material.
3. a kind of body eye cream preparation of excretion containing human umbilical cord mesenchymal stem cells, including human umbilical cord mesenchymal stem cells excretion body
And matrix, the matrix include solvent, moisturizer, thickener, chelating agent, emollient, emulsifier, skin conditioning agent, help emulsification
Agent, emulsion stabilizer, pH adjusting agent；The solvent is water.
Wherein, the human umbilical cord mesenchymal stem cells excretion body is antioxidant；
The moisturizer includes 1,3-PD, glycerin polyether -26, Sodium Hyaluronate and bright string coccus/radish root fermentation
Product filtrate；
The thickener includes xanthan gum and acrylic acid (ester) class/C10-30 alkanol propionic ester cross-linked polymer；
The chelating agent is EDETATE SODIUM；
The emollient includes PEG-75 sher butter glyceride type, sweet three ester of caprylic/capric, hydrolecithin and fertility
Phenol acetic acid esters；
The emulsifier is -6 distearate of polyglycereol；
The skin conditioning agent includes phytosterol, bisabolol and beta glucan；
The assistant for emulsifying agent is stearine；
The emulsion stabilizer is cetostearyl alcohol；
The pH adjusting agent is triethanolamine.
Specifically, by weight percentage, the content of the human umbilical cord mesenchymal stem cells excretion body is 0.005%, 1,3-
The content of propylene glycol is 4.0%, and the content of glycerin polyether -26 is 3.0%, and the content of xanthan gum is 0.1%, acrylic acid (ester)
Class/C10-30 alkylol acrylamide acid esters cross-linked polymer content is 0.1%, and the content of EDETATE SODIUM is 0.05%, PEG-75 ox
The content of oleoresin glyceride type is 3.0%, and the content of -6 distearate of polyglycereol is 2.5%, and stearine contains
Amount is 1.0%, and the content of phytosterol is 1.0%, and the content of bisabolol is 0.15%, and the content of beta glucan is
1.0%, the content of cetostearyl alcohol is 1.2%, and the content of triethanolamine is 0.1%, and the content of Sodium Hyaluronate is 0.1%,
Bright string coccus/radish root tunning filtrate content is 2.5%, and the content of sweet three ester of caprylic/capric is 3.5%, hydrogenates ovum
The content of phosphatide is 0.05%, and the content of tocopherol acetate is 0.6%, and surplus is water.
Specifically, the body of excretion containing the human umbilical cord mesenchymal stem cells eye cream preparation is water-setting agent or lotion.
Specifically, the human umbilical cord mesenchymal stem cells excretion body is human umbilical cord mesenchymal stem cells excretion body suspension.
The preparation method of the body of excretion containing the human umbilical cord mesenchymal stem cells eye cream preparation, steps are as follows:
1) raw material is divided into A group, B group, C group, D group, E group and F group, A group includes water, 1,3-PD, glycerin polyether-
26, xanthan gum, acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross-linked polymer and EDETATE SODIUM, B group include PEG-75 ox
Oleoresin glyceride type, -6 distearate of polyglycereol, caprylic/capric triglyceride, phytosterol, stearine,
Cetostearyl alcohol, hydrolecithin and tocopherol acetate, C group are bisabolol, and D group is triethanolamine, and E group is hyaluronic acid
Sodium, F group include beta glucan, bright string coccus/radish root tunning filtrate and human umbilical cord mesenchymal stem cells excretion body, will be saturating
Bright matter acid sodium is used water-swellable in advance；
2) after acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross-linked polymer and xanthan gum butanediol being dispersed,
Water is added and A group surplus material is heated to 75~80 DEG C, stirs evenly and is kept for 10 minutes；
3) B group material is heated to 80~85 DEG C, stirs evenly and keeps after ten minutes, it is uniform that C group material stirring being added；
4) step 2) products therefrom is added slowly in step 1) products therefrom, it is anti-stirs evenly progress emulsifying
It answers, it is uniform that D group material stirring is added；
5) E group material is added when being cooled to 45 DEG C, stirs evenly；
6) F group material is added when being cooled to 38 DEG C, is stirred to uniform；
7) 35 DEG C of detections are cooled to, discharging is stood, and the inspection of semifinished product is filling, warehousing finished products.
Wherein, in step 6), when F group material is added, beta glucan, bright string coccus/radish root tunning filtrate and people
Umbilical cord mesenchymal stem cells excretion body will sequentially add, and each raw material be added after stir evenly a kind of object can be added down
Material.
Embodiment 3 moves the body eye cream preparation of excretion containing human umbilical cord mesenchymal stem cells prepared by the present invention in vitro
Object safety experiment
(1) screen: experimental animal is new zealand white rabbit, and totally 8, raising adapts to 7d in laboratory conditions, in test
The interior eyes to experimental animal carry out fluorescein sodium inspection for 24 hours before starting.Eliminating has eye irritation symptom, corneal defect and knot
The animal of membrane damage.
(2) be grouped: 8 test rabbits all pass through screening, for testing the examination of human umbilical cord mesenchymal stem cells excretion body eye cream
Agent irritation, line label of going forward side by side.
(3) operate: the test same day, fixed test rabbit gently pull open left side palpebra inferior, tested material 0.1mL are instilled conjunctiva
In capsule, upper and lower eyelid is made passively to be closed 1s, to prevent tested material from losing.Other side eyes, which are not handled, makees own control.
(4) clinical examination and scoring: after instilling tested material 1h, for 24 hours, 48h, 72h to test lagophthalmos eyeball checked (
After 72h observation and record end, fluorescein sodium is added dropwise and makees further to check).It, can be whole if 72h does not occur stimulate the reaction
Only test.
If there is cornea or there are other Eye irritations to react, not recuperator in 7d, for the invertibity or not for determining the damage
Invertibity need to extend observing time, be no more than 21d, and provide the visual report of 7d, 14d, 21d.In addition to cornea, iris,
Outside conjunctiva is observed, other detrimental effects should all be recorded and be reported.The scoring that should all be damaged by 1, table in each check
The integral of standard recording Eye irritation reaction.
The standards of grading of 1, table damage
Note: " 2015 editions cosmetics safety technical specifications " (state food pharmaceuticals administration general bureau, the People's Republic of China (PRC))
Result judgement standard:
Animal corneal, iris or each leisure 1h of conjunctiva, for 24 hours, 48h, 72h observe each time point stimulate the reaction highest product
Divide mean value and recovery time evaluation, reacts classification by 2 product Eye irritation of table and determine tested material to the stimulus intensity of eye.
The reaction classification of 2 product Eye irritation of table
Note: it when cornea, iris, conjunctiva integral are 0, can be judged to nonirritant.
" 2015 editions cosmetics safety technical specifications " (state food pharmaceuticals administration general bureau, the People's Republic of China (PRC))
Table 3 be test rabbit corneal, iris, each leisure 1h of conjunctiva, for 24 hours, the stimulate the reaction of 48h and 72h observing time point
Top mean value and recovery time evaluation, it was demonstrated that eye cream of the present invention is nonirritant.
3 Ocular irritation of table reacts integral mean value
Embodiment 4, the safety of eye cream and human efficiency's experiment
(1) volunteer totally 38 are recruited, according to the status analysis of volunteer as a result, volunteer is grouped, everyone distributes 30g
Eye cream (by test product) of the present invention carries out human efficiency and security test.
(2) three moisture content at canthus, elasticity, wrinkle skin condition evaluations are carried out to every volunteer.
(3) before carrying out eye-care to every volunteer, after nursing, by test product using one week, two weeks, three weeks, surrounding into
The acquisition of row data, uses 28 days altogether.
4 test item of table and volunteer's situation
(4) data processing:
Immediate effect: the data that the data that are measured using before eye-care and nursing measure in latter hour are as immediate effect
Data carry out average value processing, improvement degree before and after eye-care are calculated, to obtain improvement number ratio and not improve number
Ratio.
5 instantaneous safety of table and Efficiency assessment table
Long Shi Xiaoguo: latter week, two weeks, three weeks, four weekly datas are used with the initial data before eye-care and by test product
Average value processing is carried out as long-term effect data, every volunteer is calculated using the improvement degree by test product, changes to obtain
Philanthropist's number ratio and do not improve number ratio.
Safety and Efficiency assessment when table 6 is long
(5) it can be concluded that by human efficiency's experiment
The eye cream preparation has stronger moisturizing effect；It is increased using the elasticity of rear volunteer's eye part skin, the eye
Frost has the effect of ultimate attainment compact；For dispelling dry line microgroove, desalinates true property line and have obvious effects on；Good security, immediately
Any discomfort sense is not all shown using rear subject when testing and is long.
It is tested by a large amount of human efficiencies, it is found that the eye cream preparation is dispelling the dry line microgroove of eye, desalinate true property line and mentioned
Significant effect in terms of high skin hydration degree.38 subject's visual sense eye part skin moisture contents are promoted immediately, skin
It moistens, shows this product to the promotion significant effect of the instant moisture content of skin；Subject more than 80% thinks that this product can be different
The instant elasticity of the promotion skin of degree；The wrinkle of subject feedback oneself more than 70% makes moderate progress, and protects in terms of smoothing wrinkle
Subject has obvious dry line microgroove and deeper true property line before managing, and eye part skin is more obscure, and after eye-care, the dry line of eye is thin
Line disappears, and skin brightness is promoted；The depth of true property line, length, width significantly reduce.It is dry to illustrate that this product can be dispelled effectively
Line microgroove desalinates true property line.
The preferred embodiment of the patent is described in detail above, but this patent is not limited to above-mentioned embodiment party
Formula within the knowledge of one of ordinary skill in the art can also be under the premise of not departing from this patent objective
It makes a variety of changes.
Claims (9)
1. a kind of body eye cream preparation of excretion containing human umbilical cord mesenchymal stem cells, which is characterized in that dry thin including human umbilical cord mesenchymal
Extracellular to secrete body and matrix, the matrix includes solvent, moisturizer, thickener, chelating agent, emollient, emulsifier, skin condition
Agent, assistant for emulsifying agent, emulsion stabilizer and pH adjusting agent；The solvent is water.
2. the body of excretion containing human umbilical cord mesenchymal stem cells eye cream preparation according to claim 1, which is characterized in that the system
Agent is water-setting agent or lotion.
3. the body of excretion containing human umbilical cord mesenchymal stem cells eye cream preparation according to claim 2, which is characterized in that the people
Umbilical cord mesenchymal stem cells excretion body is human umbilical cord mesenchymal stem cells excretion body suspension.
4. the body of excretion containing human umbilical cord mesenchymal stem cells eye cream preparation according to claim 3, which is characterized in that the people
Umbilical cord mesenchymal stem cells excretion body the preparation method comprises the following steps: supercentrifugation extract human umbilical cord mesenchymal stem cells excretion body,
Physiological saline is added after qualitative and quantitative detection to be resuspended, -80 DEG C of refrigerators save.
5. the body of excretion containing human umbilical cord mesenchymal stem cells eye cream preparation according to claim 1 to 4, which is characterized in that
The human umbilical cord mesenchymal stem cells excretion body is antioxidant；
Moisturizer includes 1,3-PD, glycerin polyether -26, Sodium Hyaluronate and bright string coccus/radish root hair in the matrix
Ferment Product filtrate；
Thickener includes xanthan gum and acrylic acid (ester) class/C10-30 alkanol propionic ester cross-linked polymer in the matrix；
Chelating agent is EDETATE SODIUM in the matrix；
Emollient includes PEG-75 sher butter glyceride type, sweet three ester of caprylic/capric, hydrolecithin and life in the matrix
Educate phenol acetic acid esters；
Emulsifier is -6 distearate of polyglycereol in the matrix；
Skin conditioning agent includes phytosterol, bisabolol and beta glucan in the matrix；
Assistant for emulsifying agent is stearine in the matrix；
Emulsion stabilizer is cetostearyl alcohol in the matrix；
PH adjusting agent is triethanolamine in the matrix.
6. the body of excretion containing human umbilical cord mesenchymal stem cells eye cream preparation according to claim 5, which is characterized in that by as follows
It is made:
7. a kind of preparation side of the body eye cream preparation of excretion containing human umbilical cord mesenchymal stem cells as described in claim 1-6 is any
Method, it is characterised in that following steps:
1) raw material is divided into A group, B group, C group, D group, E group and F group, A group includes water, 1,3-PD, glycerin polyether -26, Huang
Virgin rubber, acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross-linked polymer and EDETATE SODIUM, B group include PEG-75 Butyrospermum
Rouge glyceride type, -6 distearate of polyglycereol, caprylic/capric triglyceride, phytosterol, stearine, spermaceti
Stearyl alcohol, hydrolecithin and tocopherol acetate, C group are bisabolol, and D group is triethanolamine, and E group is Sodium Hyaluronate, F
Group includes beta glucan, bright string coccus/radish root tunning filtrate and human umbilical cord mesenchymal stem cells excretion body, by hyalomitome
Sour sodium is used water-swellable in advance；
2) it after dispersing acrylic acid (ester) class/C10-30 alkylol acrylamide acid esters cross-linked polymer and xanthan gum butanediol, is added
Water and A group surplus material are heated to 75~80 DEG C, stir evenly and are kept for 10 minutes；
3) B group material is heated to 80~85 DEG C, stirs evenly and keeps after ten minutes, it is uniform that C group material stirring being added；
4) step 2) products therefrom is slowly added into step 1) products therefrom, stirs evenly and carry out emulsifying reaction, adds
It is uniform to enter D group material stirring；
5) E group material is added when being cooled to 45 DEG C, stirs evenly；
6) F group material is added when being cooled to 38 DEG C, is stirred to uniform；
7) 35 DEG C of detections are cooled to, discharging is stood, and the inspection of semifinished product is filling, warehousing finished products.
8. the preparation method of the body of excretion containing human umbilical cord mesenchymal stem cells eye cream preparation according to claim 7, feature
It is, in step 6), when F group material is added, beta glucan, bright string coccus/filled between radish root tunning filtrate and people's umbilical cord
Matter stem cell excretion body will sequentially add, and each raw material be added after stir evenly a kind of material can be added down.
9. the body eye cream preparation of excretion containing human umbilical cord mesenchymal stem cells as described in claim 1-6 is any is improving eye line
Application in reason, raising skin hydration degree.
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| US20230117663A1 (en) * | 2021-10-17 | 2023-04-20 | ELEVAI Labs, Inc. | Exosome-based Skincare Product |
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